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Dengue AND Quercetin

María R Flores-Ocelotl, Nora H Rosas-Murrieta, Diego A Moreno, Verónica Vallejo-Ruiz, Julio Reyes-Leyva, Fabiola Domínguez, Gerardo Santos-López
BACKGROUND: Urtica dioica, Taraxacum officinale, Calea integrifolia and Caesalpinia pulcherrima are widely used all over the world for treatment of different illnesses. In Mexico, these plants are traditionally used to alleviate or counteract rheumatism and inflammatory muscle diseases. In the present study we evaluated the activity of aqueous and methanolic extracts of these four plants, on the replication of dengue virus serotype 2 (DENV2). METHODS: Extraction process was carried out in a Soxtherm® system at 60, 85 and 120 °C; a chemical fractionation in silica gel chromatography was performed and compounds present in the active fractions were identified by HPLC-DAD-ESI/MSn...
March 16, 2018: BMC Complementary and Alternative Medicine
Rodolfo S Barboza, Ligia M M Valente, Thiago Wolff, Iranaia Assunção-Miranda, Rômulo L S Neris, Iris P Guimarães-Andrade, Mário Gomes
The defatted fractions of the Faramea hyacinthina and F. truncata (Rubiaceae) leaf MeOH extracts showed in vitro non-cytotoxic and anti-dengue virus serotype 2 (DENV2) activity in human hepatocarcinoma cell lineage (HepG2). Submitting these fractions to the developed RP-SPE method allowed isolating the antiviral flavanone (2S)-isosakuranetin-7-O-β-d-apiofuranosyl-(1→6)-β-d-glucopyranoside (1) from both species and yielded less active sub-fractions. The new diastereoisomeric epimer pair (2S) + (2R) of 5,3',5'-trihydroxyflavanone-7-O-β-d-apiofuranosyl-(1→6)-β-d-glucopyranoside (2a/2b) from F...
February 2018: Chemistry & Biodiversity
Ankita Pan, Wuan Geok Saw, Malathy Sony Subramanian Manimekalai, Ardina Grüber, Shin Joon, Tsutomu Matsui, Thomas M Weiss, Gerhard Grüber
Dengue virus (DENV), which has four serotypes (DENV-1 to DENV-4), is the causative agent of the viral infection dengue. DENV nonstructural protein 3 (NS3) comprises a serine protease domain and an RNA helicase domain which has nucleotide triphosphatase activities that are essential for RNA replication and viral assembly. Here, solution X-ray scattering was used to provide insight into the overall structure and flexibility of the entire NS3 and its recombinant helicase and protease domains for Dengue virus serotypes 2 and 4 in solution...
May 1, 2017: Acta Crystallographica. Section D, Structural Biology
Shanmugam Anusuya, M Michael Gromiha
Dengue is an important public health problem in tropical and subtropical regions of the world. Neither vaccine nor an antiviral medication is available to treat dengue. This insists the need of drug discovery for dengue. In order to find a potent lead molecule, RNA-dependent RNA polymerase which is essential for dengue viral replication is chosen as a drug target. As Quercetin showed antiviral activity against several viruses, quercetin derivatives developed by combinatorial library synthesis and mined from PubChem databases were screened for a potent anti-dengue viral agent...
October 2017: Journal of Biomolecular Structure & Dynamics
Nurul Azira Ismail, Siti Azma Jusoh
Dengue infections are currently estimated to be 390 million cases annually. Yet, there is no vaccine or specific therapy available. Envelope glycoprotein E (E protein) of DENV mediates viral attachment and entry into the host cells. Several flavonoids have been shown to inhibit HIV-1 and hepatitis C virus entry during the virus-host membrane fusion. In this work, molecular docking method was employed to predict the binding of nine flavonoids (baicalin, baicalein, EGCG, fisetin, glabranine, hyperoside, ladanein, quercetin and flavone) to the soluble ectodomain of DENV type 2 (DENV2) E protein...
March 11, 2016: Interdisciplinary Sciences, Computational Life Sciences
K H Chiow, M C Phoon, Thomas Putti, Benny K H Tan, Vincent T Chow
OBJECTIVE: To evaluate the in vitro activities of the ethyl acetate (EA) fraction of Houttuynia cordata (H. cordata) Thunb. (Saururaceae) and three of its constituent flavonoids (quercetin, quercitrin and rutin) against murine coronavirus and dengue virus (DENV). METHODS: The antiviral activities of various concentrations of the EA fraction of H. cordata and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus (MHV) and DENV type 2 (DENV-2)...
January 2016: Asian Pacific Journal of Tropical Medicine
Lorena Ramos Freitas de Sousa, Hongmei Wu, Liliane Nebo, João Batista Fernandes, Maria Fátima das Graças Fernandes da Silva, Werner Kiefer, Manuel Kanitz, Jochen Bodem, Wibke E Diederich, Tanja Schirmeister, Paulo Cezar Vieira
NS2B-NS3 is a serine protease of the Dengue virus considered a key target in the search for new antiviral drugs. In this study flavonoids were found to be inhibitors of NS2B-NS3 proteases of the Dengue virus serotypes 2 and 3 with IC50 values ranging from 15 to 44 μM. Agathisflavone (1) and myricetin (4) turned out to be noncompetitive inhibitors of dengue virus serotype 2 NS2B-NS3 protease with Ki values of 11 and 4.7 μM, respectively. Docking studies propose a binding mode of the flavonoids in a specific allosteric binding site of the enzyme...
February 1, 2015: Bioorganic & Medicinal Chemistry
Padmanaban Senthilvel, Pandian Lavanya, Kalavathi Murugan Kumar, Rayapadi Swetha, Parimelzaghan Anitha, Susmita Bag, Sundaramoorthy Sarveswari, Vijayaparthasarathi Vijayakumar, Sudha Ramaiah, Anand Anbarasu
UNLABELLED: Dengue virus belongs to the virus family Flaviviridae. Dengue hemorrhagic disease caused by dengue virus is a public health problem worldwide. The viral non structural 2B and 3 (NS2B-NS3) protease complex is crucial for virus replication and hence, it is considered to be a good anti-viral target. Leaf extracts from Carica papaya is generally prescribed for patients with dengue fever, but there are no scientific evidences for its anti-dengue activity; hence we intended to investigate the anti-viral activity of compounds present in the leaves of Carica papaya against dengue 2 virus (DENV-2)...
2013: Bioinformation
Emmanuel Viana Pontual, Thiago Henrique Napoleão, Caio Rodrigo Dias de Assis, Ranilson de Souza Bezerra, Haroudo Satiro Xavier, Daniela Maria do Amaral Ferraz Navarro, Luana Cassandra Breitenbach Barroso Coelho, Patrícia Maria Guedes Paiva
Aedes aegypti control is crucial to reducing dengue fever. Aedes aegypti larvae have developed resistance to organophosporous insecticides and the use of natural larvicides may help manage larval resistance by increasing elements in insecticide rotation programs. Here, we report on larvicidal activity of Moringa oleifera flower extract against A. aegypti L(1), L(2), L(3), and L(4) as well as the effect of flower extract on gut trypsin and whole-larval acetylcholinesterase from L(4.) In addition, the heated flower extract was investigated for larvicidal activity against L(4) and effect on larval gut trypsin...
March 2012: Archives of Insect Biochemistry and Physiology
Keivan Zandi, Boon-Teong Teoh, Sing-Sin Sam, Pooi-Fong Wong, Mohd Rais Mustafa, Sazaly Abubakar
BACKGROUND: Dengue is a major mosquito-borne disease currently with no effective antiviral or vaccine available. Effort to find antivirals for it has focused on bioflavonoids, a plant-derived polyphenolic compounds with many potential health benefits. In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. Anti-dengue activity of these compounds was determined at different stages of DENV-2 infection and replication cycle...
2011: Virology Journal
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