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https://www.readbyqxmd.com/read/27910552/versatile-wetting-measurement-of-microplate-wells
#1
Enoch Ming Wei Ng, Brandon Huey-Ping Cheong, Yang Yu, Oi Wah Liew, Tuck Wah Ng
A method to measure the contact angle, which is indicative of wetting, using small liquid volumes dispensed directly on microplate wells is described and demonstrated. Experiments with enhanced green protein samples of volumes 4.4-6 μl showed no measured variance in the contact angle. Experiments with phosphate buffer solution with varied concentrations of a non-ionic detergent (Tween 20) dissolved, however, revealed smaller contact angles with increased detergent concentration. It is experimentally shown that drops can be located up to 7° from the lowest position of the well without affecting the accuracy of contact angle measurements...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27909523/comparison-of-the-influence-of-right-atrial-septal-pacing-and-appendage-pacing-on-an-atrial-function-and-atrial-fibrillation-in-the-clinical-situation
#2
Mariko Tanaka Md, Kanae Su Md, Maki Oi Md, Yasuyo Motohashi Md, Kousuke Takahashi Md, Euihong Ko Md, Koji Hanazawa Md, Mamoru Toyofuku Md, Masahiko Kitada Md, Yousuke Yuzuki Md, Takashi Tamura Md
INTRODUCTION: Recently right atrial septal (RAS) pacing is often selected, but the benefit brought by RAS pacing has not been clear. The aim of this study was to evaluate the differences between RAS pacing and right appendage (RAA) pacing. METHODS: 223 consecutive new patients with a right atrial (RA) electrode during the period from January 2004 to December 2012 were studied retrospectively. They were observed regularly at our pacemaker (PM) clinic, and we measured paced P - sensed QRS interval (pPQi) and left atrial dimension (LAD) during the observation period, furthermore the onset of atrial fibrillation (AF) was assessed...
June 2016: Journal of Atrial Fibrillation
https://www.readbyqxmd.com/read/27894323/health-related-quality-of-life-and-a-cost-utility-simulation-of-adults-in-the-uk-with-osteogenesis-imperfecta-x-linked-hypophosphatemia-and-fibrous-dysplasia
#3
Lydia Forestier-Zhang, Laura Watts, Alison Turner, Harriet Teare, Jane Kaye, Joe Barrett, Cyrus Cooper, Richard Eastell, Paul Wordsworth, Muhammad K Javaid, Rafael Pinedo-Villanueva
BACKGROUND: Health-related quality of life of adults with osteogenesis imperfecta (OI), fibrous dysplasia (FD) and X-linked hypophosphatemia (XLH) remains poorly described. The aim of this study was to describe the HRQoL of adults with osteogenesis imperfecta, fibrous dysplasia and X-linked hypophophataemia and perform a cost-utility simulation to calculate the maximum cost that a health care system would be willing to pay for a hypothetical treatment of a rare bone disease. RESULTS: Participants completed the EQ-5D-5 L questionnaire between September 2014 and March 2016...
November 28, 2016: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/27885708/oldest-medical-description-of-osteogenesis-imperfecta-17th-century-france
#4
Philippe Charlier, Antonio Perciaccante, Raffaella Bianucci
Osteogenesis imperfecta (OI), also known as Lobstein's syndrome or Vrolik's syndrome, comprises a heterogeneous group of rare genetic connective tissue disorders. It is characterized by increased bone fragility, low bone mass, and susceptibility to bone fractures of variable severity. Originally named "osteomalacia congenita," the condition was first medically described in a family by Ekman in 1778. Here, we report a 17th century medical account from France, which predates Eckman's doctoral dissertation by about a century...
November 7, 2016: Clinical Anatomy
https://www.readbyqxmd.com/read/27884655/zika-virus-infection-in-dexamethasone-immunosuppressed-mice-demonstrating-disseminated-infection-with-multi-organ-involvement-including-orchitis-effectively-treated-by-recombinant-type-i-interferons
#5
Jasper Fuk-Woo Chan, Anna Jinxia Zhang, Chris Chung-Sing Chan, Cyril Chik-Yan Yip, Winger Wing-Nga Mak, Houshun Zhu, Vincent Kwok-Man Poon, Kah-Meng Tee, Zheng Zhu, Jian-Piao Cai, Jessica Oi-Ling Tsang, Kenn Ka-Heng Chik, Feifei Yin, Kwok-Hung Chan, Kin-Hang Kok, Dong-Yan Jin, Rex Kwok-Him Au-Yeung, Kwok-Yung Yuen
BACKGROUND: Disseminated or fatal Zika virus (ZIKV) infections were reported in immunosuppressed patients. Existing interferon-signaling/receptor-deficient mouse models may not be suitable for evaluating treatment effects of recombinant interferons. METHODS: We developed a novel mouse model for ZIKV infection by immunosuppressing BALB/c mice with dexamethasone. RESULTS: Dexamethasone-immunosuppressed male mice (6-8weeks) developed disseminated infection as evidenced by the detection of ZIKV-NS1 protein expression and high viral loads in multiple organs...
November 12, 2016: EBioMedicine
https://www.readbyqxmd.com/read/27882025/no-inhalation-in-combination-with-high-frequency-ventilation-treatment-in-the-treatment-of-neonatal-severe-respiratory-failure
#6
Xiaohui Guo, Yanfeng Sun, Jing Miao, Min Cui, Jiangbo Wang, Shuzhen Han
OBJECTIVE: To discuss over NO inhalation (iNO) in combination with high frequency ventilation treatment in relieving clinical symptoms and respiratory state of patients with neonatal severe respiratory failure. METHODS: Ninety newborns with severe respiratory failure who received treatment in our hospital were selected for this study. They were divided into research group and control group according to visiting time. Patients in the control group were given conventional treatment in combination with high-frequency oscillatory ventilation, while patients in the research group were given iNO for treatment additionally besides the treatment the same as the control group...
September 2016: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/27876255/patient-and-perioperative-variables-affecting-30-day-readmission-for-surgical-complications-after-hip-and-knee-arthroplasties-a-matched-cohort-study
#7
Benjamin F Ricciardi, Kathryn K Oi, Steven B Daines, Yuo-Yu Lee, Amethia D Joseph, Geoffrey H Westrich
BACKGROUND: Changes in reimbursement for total hip and knee arthroplasties (THA and TKA) have placed increased financial burden of early readmission on hospitals and surgeons. Our purpose was to characterize factors of 30-day readmission for surgical complications after THA and TKA at a single, high-volume orthopedic specialty hospital. METHODS: Patients with a diagnosis of osteoarthritis and who were readmitted within 30 days of their unilateral primary THA or TKA procedure between 2010 and 2014...
October 21, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/27874934/effect-of-nitric-oxide-inhalation-for-the-treatment-of-neonatal-pulmonary-hypertension
#8
H-W Wu, Z-G Li, G Liu, G-Z Lu, H-Y Liang
OBJECTIVE: To investigate the effect of nitric oxide (NO) inhalation for the treatment of neonatal pulmonary hypertension. PATIENTS AND METHODS: Eighty-six patients with neonatal pulmonary hypertension who were treated for the first time Xuzhou Children's Hospital from January 2013 to January 2016 were selected and randomly divided into the observation group and control group, with 43 cases each. Patients in the control group were treated with high-frequency oscillatory ventilation, while those in the observation group were treated with high-frequency oscillatory ventilation combined with inhalational NO therapy...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27869851/calibration-of-oblique-incidence-reflectivity-difference-for-label-free-detection-of-a-molecular-layer
#9
Chenggang Zhu, Ru Chen, Yuzhangyang Zhu, Xu Wang, Xiangdong Zhu, Lan Mi, Fengyun Zheng, Yiyan Fei
Oblique-incidence reflectivity difference (OI-RD) is a form of polarization-modulation ellipsometry that measures properties of thin films on a solid surface through the change in polarization state of light upon reflection from the surface. The measurement accuracy depends on the precision of the phase modulation amplitude and azimuthal alignments of key polarizing optical elements and, thus, requires careful calibration. In the present work, we describe robust methods of such calibrations that enable precise determination of the modulation amplitude and static retardation of a phase modulator and azimuths of key polarizing optics in an OI-RD system...
November 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/27867562/analysis-of-risk-factors-for-and-the-prognosis-of-postoperative-acute-respiratory-distress-syndrome-in-patients-with-stanford-type-a-aortic-dissection
#10
Mei-Fang Chen, Liang-Wan Chen, Hua Cao, Yong Lin
BACKGROUND: To explore the risk factors for and the prognosis of postoperative acute respiratory distress syndrome (ARDS) in patients with Stanford type A aortic dissection (AD). METHODS: This retrospective nested case-control study included 527 Stanford type A AD patients who were divided into ARDS groups and non-ARDS groups. The clinical features of the groups were examined. RESULTS: The fifty-nine patients in the ARDS group exhibited extended durations of cardiopulmonary bypass (CPB) (P=0...
October 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27867224/approximating-the-little-grothendieck-problem-over-the-orthogonal-and-unitary-groups
#11
Afonso S Bandeira, Christopher Kennedy, Amit Singer
The little Grothendieck problem consists of maximizing Σ ij Cijxixj for a positive semidef-inite matrix C, over binary variables xi ∈ {±1}. In this paper we focus on a natural generalization of this problem, the little Grothendieck problem over the orthogonal group. Given C ∈ ℝ (dn) × (dn) a positive semidefinite matrix, the objective is to maximize [Formula: see text] restricting Oi to take values in the group of orthogonal matrices [Formula: see text], where Cij denotes the (ij)-th d × d block of C...
November 2016: Mathematical Programming
https://www.readbyqxmd.com/read/27864101/targeted-exome-sequencing-identifies-novel-compound-heterozygous-mutations-in-p3h1-in-a-fetus-with-osteogenesis-imperfecta-type-viii
#12
Yanru Huang, Libin Mei, Weigang Lv, Haoxian Li, Rui Zhang, Qian Pan, Hu Tan, Jing Guo, Xiaomei Luo, Chen Chen, Desheng Liang, Lingqian Wu
Osteogenesis imperfecta (OI) is a highly clinically and genetically heterogeneous group of disorders. It is difficult to identify severe OI in the perinatal period. Here, a Chinese woman with a suspected history of fetal OI was referred to our institution at 19weeks of gestation, due to ultrasound inspection during antenatal screening, which revealed bulbous metaphyses, short humeri, and short thick bent femora in the fetus. Using targeted exome sequencing of 248 genes known to be involved in skeletal system diseases, we identified novel compound heterozygous mutation in the P3H1 gene in the fetus with OI type VIII: c...
November 15, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27854143/assessment-of-acute-head-injury-in-an-emergency-department-population-using-sport-concussion-assessment-tool-3rd-edition
#13
Abdullah Bin Zahid, Molly E Hubbard, Vikalpa M Dammavalam, David Y Balser, Gritz Pierre, Amie Kim, Radek Kolecki, Talha Mehmood, Stephen P Wall, Spiros G Frangos, Paul P Huang, David E Tupper, William Barr, Uzma Samadani
Sport Concussion Assessment Tool version 3 (SCAT-3) is one of the most widely researched concussion assessment tools in athletes. Here normative data for SCAT3 in nonathletes are presented. The SCAT3 was administered to 98 nonathlete healthy controls, as well as 118 participants with head-injury and 46 participants with other body trauma (OI) presenting to the ED. Reference values were derived and classifier functions were built to assess the accuracy of SCAT3. The control population had a mean of 2.30 (SD = 3...
November 17, 2016: Applied Neuropsychology. Adult
https://www.readbyqxmd.com/read/27832015/intramedullary-nailing-with-supplemental-plate-and-screw-fixation-of-long-bones-of-patients-with-osteogenesis-imperfecta-operative-technique-and-preliminary-results
#14
Jeanne M Franzone, Richard W Kruse
Although intramedullary fixation is the standard form of surgical fixation of the long bones of children with osteogenesis imperfecta (OI), it remains fraught with complications. Implant breakage, implant cut out, long bone fracture, nonunion, and rod bending have all been described as complications of intramedullary long bone fixation in children with OI. Supplemental fixation techniques represent an attempt to decrease these risks of surgical implant failure of intramedullary devices. A supplemental plate and screw construct at a fracture or an osteotomy site in addition to an intramedullary device in the long bone segment is one such form of supplemental fixation...
November 9, 2016: Journal of Pediatric Orthopedics. Part B
https://www.readbyqxmd.com/read/27830459/comparison-of-radial-brachial-and-femoral-accesses-using-hemostatic-devices-for-percutaneous-coronary-intervention
#15
Masaya Otsuka, Nobuo Shiode, Yasuhisa Nakao, Yuki Ikegami, Yusuke Kobayashi, Arinori Takeuchi, Ayako Harima, Tadanao Higaki, Kuniomi Oi, Kazuoki Dai, Tomoharu Kawase, Yasuharu Nakama, Kazuyoshi Suenari, Kenji Nishioka, Koyu Sakai, Yuji Shimatani, Yoshiko Masaoka, Ichiro Inoue
Some studies have suggested that radial access (RA) for percutaneous coronary intervention (PCI) reduces vascular complications and bleeding compared to femoral access (FA). The purpose of this study was to investigate the routine use of hemostatic devices and bleeding complications among RA, brachial access (BA), and FA. Between January 2015 and December 2015, 298 patients treated for PCI with RA were compared with 158 patients using BA and 206 patients using FA. The radial sheath was routinely removed with ADAPTY, the brachial sheath with BLEED SAFE, and the femoral sheath with Perclose ProGlide...
November 9, 2016: Cardiovascular Intervention and Therapeutics
https://www.readbyqxmd.com/read/27822779/appropriate-timing-of-surgery-for-neonates-with-congenital-diaphragmatic-hernia-early-or-delayed-repair
#16
Hiroomi Okuyama, Noriaki Usui, Masahiro Hayakawa, Tomoaki Taguchi
PURPOSE: This study was aimed to evaluate the influence of timing of surgery on patient outcomes, and to clarify appropriate timing of surgery in neonates with congenital diaphragmatic hernia (CDH). METHODS: A total of 477 neonates with isolated CDH were included. Patients were classified into two groups by timing of surgery: early repair (ER) (≤48 h) and delayed repair (DR) (>48 h). The primary outcome was 90-day survival, with treatment duration (ventilation, oxygen, and hospitalization) being a secondary outcome...
November 7, 2016: Pediatric Surgery International
https://www.readbyqxmd.com/read/27817768/-clinical-effect-of-high-frequency-oscillatory-ventilation-combined-with-pulmonary-surfactant-in-treatment-of-neonatal-severe-meconium-aspiration-syndrome-complicated-by-pulmonary-hemorrhage
#17
Jing Huang, Xin-Zhu Lin, Zhi Zheng
OBJECTIVE: To study the clinical effect and safety of high-frequency oscillatory ventilation (HFOV) combined with pulmonary surfactant (PS) in the treatment of neonatal severe meconium aspiration syndrome (MAS) complicated by neonatal pulmonary hemorrhage (NPH). METHODS: A total of 48 children with severe MAS complicated by NPH were enrolled, and a retrospective analysis was performed for the clinical effects of HFOV+PS (trial group, 25 children) and HFOV alone (control group, 23 children)...
November 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/27817767/-clinical-effects-of-different-ways-of-mechanical-ventilation-combined-with-pulmonary-surfactant-in-treatment-of-acute-lung-injury-acute-respiratory-distress-syndrome-in-neonates-a-comparative-analysis
#18
Ming Chang, Hong-Yan Lu, Hong Xiang, Hou-Ping Lan
OBJECTIVE: To compare the therapeutic effects of high-frequency oscillatory ventilation+pulmonary surfactant (HFOV+PS), conventional mechanical ventilation+pulmonary surfactant (CMV+PS), and conventional mechanical ventilation (CMV) alone for acute lung injury/acute respiratory distress syndrome (ALI/ARDS) in neonates. METHODS: A total of 136 neonates with ALI/ARDS were enrolled, among whom 73 had ALI and 63 had ARDS. They were divided into HFOV+PS group (n=45), CMV+PS group (n=53), and CMV group (n=38)...
November 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/27816290/clinical-and-arthrometric-outcomes-of-an-anatomic-outside-in-single-bundle-anterior-cruciate-ligament-reconstruction-using-a-retrodrill
#19
David Figueroa, Rafael Calvo, Francisco Figueroa, Daniel Paccot, Guillermo Izquierdo, Nelson Morales
BACKGROUND: The main option to perform an anatomic anterior cruciate ligament (ACL) reconstruction is the anteromedial portal (AMP) technique. It has several reported complications (iatrogenic chondral injury, posterior-wall blowout, short sockets, increased risk of injury to common peroneal nerve). In an attempt to avoid these complications the outside-in (OI) technique was revived with the addition of a retrodrill. The aim of this study is to evaluate the clinical and arthrometric outcomes of a series of anatomical OI single bundle ACL reconstruction using a retrodrill...
November 2, 2016: Knee
https://www.readbyqxmd.com/read/27813341/pharmacological-and-biological-therapeutic-strategies-for-osteogenesis-imperfecta
#20
Ronit Marom, Yi-Chien Lee, Ingo Grafe, Brendan Lee
Osteogenesis imperfecta (OI) is a connective tissue disorder characterized by bone fragility, low bone mass, and bone deformities. The majority of cases are caused by autosomal dominant pathogenic variants in the COL1A1 and COL1A2 genes that encode type I collagen, the major component of the bone matrix. The remaining cases are caused by autosomal recessively or dominantly inherited mutations in genes that are involved in the post-translational modification of type I collagen, act as type I collagen chaperones, or are members of the signaling pathways that regulate bone homeostasis...
November 3, 2016: American Journal of Medical Genetics. Part C, Seminars in Medical Genetics
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