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tie2-expressing monocytes

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https://www.readbyqxmd.com/read/27842980/establishment-characterization-and-long-term-culture-of-human-endocrine-pancreas-derived-microvascular-endothelial-cells
#1
Valeria Sordi, Anna Ferri, Valentina Ceserani, Emilio Ciusani, Erica Dugnani, Silvia Pellegrini, Rita Nano, Lorenza Pecciarini, Augusto Pessina, Luisa Pascucci, Lorenzo Piemonti, Giulio Alessandri
BACKGROUND: In vitro primary cultures of microvascular endothelial cells from endocrine pancreas are difficult to obtain, but can be a very helpful tool for studies of islet biology, transplantation and regenerative medicine. METHODS: We applied a protocol recently described for the isolation and culture of brain microvascular endothelial cells (EC) on human pancreatic islets. EC obtained were characterized in terms of morphological (light and transmission electron microscopy), phenotypical (by immunofluorescence and flow cytometry) and functional (cord formation assay and protein secretion by multiplex bead-based assay) characteristics...
November 11, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27754101/os-21-06-three-month-exposure-to-endothelin-1-overexpression-increases-blood-pressure-and-causes-small-artery-injury
#2
Pierre Paradis, Suellen C Coelho, Sofiane Ouerd, Júlio C Fraulob-Aquino, Stefan Offermanns, Ernesto L Schiffrin
OBJECTIVE: The mechanisms of blood pressure (BP) regulation by endothelin (ET)-1 produced by endothelial cells are complex and remain unclear. Recently, we developed a transgenic mouse with tamoxifen-inducible endothelium-restricted human ET-1 overexpression (ieET-1) using Cre/loxP technology. ieET-1 mice exhibited a BP increase after three weeks of induction in an ET type A receptor-dependent manner, in absence of evident vascular injury. It is unknown whether long-term exposure to ET-1 overexpression results in persistent BP elevation and vascular injury...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27686285/cthrc1-promotes-angiogenesis-by-recruiting-tie2-expressing-monocytes-to-pancreatic-tumors
#3
Jaemin Lee, Jinhoi Song, Eun-Soo Kwon, Seongyea Jo, Min Kyung Kang, Yeon Jeong Kim, Yeonsil Hwang, Hosung Bae, Tae Heung Kang, Suhwan Chang, Hee Jun Cho, Song Cheol Kim, Seokho Kim, Sang Seok Koh
CTHRC1 (collagen triple-helix repeat-containing 1), a protein secreted during the tissue-repair process, is highly expressed in several malignant tumors, including pancreatic cancer. We recently showed that CTHRC1 has an important role in the progression and metastasis of pancreatic cancer. Although CTHRC1 secretion affects tumor cells, how it promotes tumorigenesis in the context of the microenvironment is largely unknown. Here we identified a novel role of CTHRC1 as a potent endothelial activator that promotes angiogenesis by recruiting bone marrow-derived cells to the tumor microenvironment during tumorigenesis...
September 30, 2016: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/27643026/os-21-06-three-month-exposure-to-endothelin-1-overexpression-increases-blood-pressure-and-causes-small-artery-injury
#4
Pierre Paradis, Suellen C Coelho, Sofiane Ouerd, Júlio C Fraulob-Aquino, Stefan Offermanns, Ernesto L Schiffrin
OBJECTIVE: The mechanisms of blood pressure (BP) regulation by endothelin (ET)-1 produced by endothelial cells are complex and remain unclear. Recently, we developed a transgenic mouse with tamoxifen-inducible endothelium-restricted human ET-1 overexpression (ieET-1) using Cre/loxP technology. ieET-1 mice exhibited a BP increase after three weeks of induction in an ET type A receptor-dependent manner, in absence of evident vascular injury. It is unknown whether long-term exposure to ET-1 overexpression results in persistent BP elevation and vascular injury...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27602755/ibrutinib-modifies-the-function-of-monocyte-macrophage-population-in-chronic-lymphocytic-leukemia
#5
Stefania Fiorcari, Rossana Maffei, Valentina Audrito, Silvia Martinelli, Elisa Ten Hacken, Patrizia Zucchini, Giulia Grisendi, Leonardo Potenza, Mario Luppi, Jan A Burger, Silvia Deaglio, Roberto Marasca
In lymphoid organs, nurse-like cells (NLCs) show properties of tumor-associated macrophages, playing a crucial role in chronic lymphocytic leukemia (CLL) cell survival. Ibrutinib, a potent inhibitor of Bruton's tyrosine kinase (BTK), is able to counteract pro-survival signals in CLL cells. Since the effects on CLL cells have been studied in the last years, less is known about the influence of ibrutinib on NLCs properties. We sought to determine how ibrutinib modifies NLCs functions focusing on the balance between immunosuppressive and inflammatory features...
September 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27471631/chemotherapy-of-colorectal-liver-metastases-induces-a-rapid-rise-in-intermediate-blood-monocytes-which-predicts-treatment-response
#6
Dominic Schauer, Patrick Starlinger, Lejla Alidzanovic, Philipp Zajc, Thomas Maier, Alexandra Feldman, Robin Padickakudy, Elisabeth Buchberger, Vanessa Elleder, Andreas Spittler, Judith Stift, Lorand Pop, Birgit Gruenberger, Thomas Gruenberger, Christine Brostjan
We have previously reported that intermediate monocytes (CD14(++)/CD16(+)) were increased in colorectal cancer (CRC) patients, while the subset of pro-angiogenic TIE2-expressing monocytes (TEMs) was not significantly elevated. This study was designed to evaluate changes in frequency and function of intermediate monocytes and TEMs during chemotherapy and anti-angiogenic cancer treatment and their relation to treatment response. Monocyte populations were determined by flow cytometry in 60 metastasized CRC (mCRC) patients who received neoadjuvant chemotherapy with or without bevacizumab...
June 2016: Oncoimmunology
https://www.readbyqxmd.com/read/27281335/tie2-expressing-monocytes-in-the-spleen-of-patients-with-primary-myelofibrosis
#7
Rita Campanelli, Gabriela Fois, Paolo Catarsi, Valentina Poletto, Laura Villani, Benedetta Gaia Erba, Luigi Maddaluno, Basilio Jemos, Silvia Salmoiraghi, Paola Guglielmelli, Vittorio Abbonante, Christian Andrea Di Buduo, Alessandra Balduini, Alessandra Iurlo, Giovanni Barosi, Vittorio Rosti, Margherita Massa
Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis...
2016: PloS One
https://www.readbyqxmd.com/read/27111031/prognostic-significance-of-tie2-expressing-monocytes-in-hilar-cholangiocarcinoma
#8
Georgi Atanasov, Hans-Michael Hau, Corinna Dietel, Christian Benzing, Felix Krenzien, Andreas Brandl, Julianna P Englisch, Georg Wiltberger, Katrin Schierle, Simon C Robson, Anja Reutzel-Selke, Sven Jonas, Andreas Pascher, Johann Pratschke, Moritz Schmelzle
BACKGROUND AND OBJECTIVES: Angiopoietins (Angs) play a pivotal role in angiogenesis and inflammation, and are associated with prognosis in malignancies. Monocyte express Ang-receptor TIE2 and correlate with prognosis in cancer. We aimed to investigate the prognostic value of Angs and TIE2-expressing monocytes (TEMs) in cholangiocarcinoma. METHODS: We analyzed surgically resected tumor specimens of hilar cholangiocarcinoma (n = 47) for distribution of Angs (Ang 1/Ang 2) and TEMs, as defined by co-expression of CD14 and Ang receptor TIE2...
July 2016: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/26965451/novel-met-tie2-vegfr2-inhibitor-altiratinib-inhibits-tumor-growth-and-invasiveness-in-bevacizumab-resistant-glioblastoma-mouse-models
#9
Yuji Piao, Soon Young Park, Verlene Henry, Bryan D Smith, Ningyi Tiao, Daniel L Flynn, John F de Groot
BACKGROUND: Glioblastoma highly expresses the proto-oncogene MET in the setting of resistance to bevacizumab. MET engagement by hepatocyte growth factor (HGF) results in receptor dimerization and autophosphorylation mediating tumor growth, invasion, and metastasis. Evasive revascularization and the recruitment of TIE2-expressing macrophages (TEMs) are also triggered by anti-VEGF therapy. METHODS: We investigated the activity of altiratinib (a novel balanced inhibitor of MET/TIE2/VEGFR2) against human glioblastoma stem cell lines in vitro and in vivo using xenograft mouse models...
September 2016: Neuro-oncology
https://www.readbyqxmd.com/read/26910374/soluble-tie2-overrides-the-heightened-invasion-induced-by-anti-angiogenesis-therapies-in-gliomas
#10
Nahir Cortes-Santiago, Mohammad B Hossain, Konrad Gabrusiewicz, Xuejun Fan, Joy Gumin, Frank C Marini, Marta M Alonso, Frederick Lang, W K Yung, Juan Fueyo, Candelaria Gomez-Manzano
Glioblastoma recurrence after treatment with the anti-vascular endothelial growth factor (VEGF) agent bevacizumab is characterized by a highly infiltrative and malignant behavior that renders surgical excision and chemotherapy ineffective. Our group has previously reported that Tie2-expressing monocytes (TEMs) are aberrantly present at the tumor/normal brain interface after anti-VEGF therapies and their significant role in the invasive outgrowth of these tumors. Here, we aimed to further understand the mechanisms leading to this pro-invasive tumor microenvironment...
March 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/26748243/distinct-rna-transcriptome-patterns-are-potentially-associated-with-angiogenesis-in-tie2-expressing-monocytes
#11
Xinjing Wang, Zhiyuan Dai, Xiaoli Wu, Kai Wang, Xipeng Wang
Tie2-expressing Monocytes (TEMs) were previously identified as a novel subset of monocytes and were believed to have prominent pro-angiogenesis activities in human tumors. While the molecular mechanism of the angiogenesis promoting capacity of TEMs remains unclear. RNA transcriptome pattern, including non-coding RNAs as microRNA (miRNA) and long non-coding RNA (lncRNA), plays important role in cell differentiation and functions. However, little is known about the transcriptome patterns of TEMs, including those non-coding RNAs...
April 10, 2016: Gene
https://www.readbyqxmd.com/read/26686201/a-phase-i-trial-of-ang1-2-tie2-inhibitor-trebaninib-amg386-and-temsirolimus-in-advanced-solid-tumors-pjc008-nci%C3%A2-9041
#12
Joanne W Chiu, Sebastien J Hotte, Christian K Kollmannsberger, Daniel J Renouf, David W Cescon, David Hedley, Sue Chow, Jeffrey Moscow, Zhuo Chen, Meghan Perry, Ivan Diaz-Padilla, David Tan, Hal Hirte, Elaine McWhirter, Helen Chen, Lillian L Siu, Philippe L Bedard
BACKGROUND: There is crosstalk between the ANG-Tie2 and the PI3K/Akt/mTOR pathways. Combined ANG1/2 and mTOR blockade may have additive anti-cancer activity. The combination of trebananib, an inhibitor of ANG1/2-Tie2 interaction, with temsirolimus was evaluated in patients with advanced solid tumors to determine tolerability, maximum tolerated dose (MTD), and preliminary antitumor activity. METHODS: Patients were enrolled using 3 + 3 design, and were given intravenous trebananib and temsirolimus on Day 1, 8, 15 and 22 of a 28-day cycle...
February 2016: Investigational New Drugs
https://www.readbyqxmd.com/read/26599011/tie2-expressing-monocytes-are-associated-with-identification-and-prognoses-of-hepatitis-b-virus-related-hepatocellular-carcinoma-after-resection
#13
Yi-Feng He, Chao-Qun Wang, Yao Yu, Jing Qian, Kang Song, Qi-Man Sun, Jian Zhou
BACKGROUND: Tie2-expressing monocytes (TEMs) are found in various tumors, involved in forming tumor blood vessels and expressing several important proangiogenic factors. The goals of this study were to evaluate the value of TEMs in diagnosing and predicting the prognosis of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). METHODS: Flow cytometry was performed to identify and count TEMs in peripheral blood monocytes from HCC patients (n = 84) receiving hepatectomy, HBV cirrhotic patients (n = 21), benign tumors patients (n = 15) and healthy volunteers (n = 23)...
2015: PloS One
https://www.readbyqxmd.com/read/25961566/chronic-inhibition-of-pde5-limits-pro-inflammatory-monocyte-macrophage-polarization-in-streptozotocin-induced-diabetic-mice
#14
Mary Anna Venneri, Elisa Giannetta, Giuseppe Panio, Rita De Gaetano, Daniele Gianfrilli, Riccardo Pofi, Silvia Masciarelli, Francesco Fazi, Manuela Pellegrini, Andrea Lenzi, Fabio Naro, Andrea M Isidori
Diabetes mellitus is characterized by changes in endothelial cells that alter monocyte recruitment, increase classic (M1-type) tissue macrophage infiltration and lead to self-sustained inflammation. Our and other groups recently showed that chronic inhibition of phosphodiesterase-5 (PDE5i) affects circulating cytokine levels in patients with diabetes; whether PDE5i also affects circulating monocytes and tissue inflammatory cell infiltration remains to be established. Using murine streptozotocin (STZ)-induced diabetes and in human vitro cell-cell adhesion models we show that chronic hyperglycemia induces changes in myeloid and endothelial cells that alter monocyte recruitment and lead to self-sustained inflammation...
2015: PloS One
https://www.readbyqxmd.com/read/25568866/tie2-expressing-monocytes-a-possible-cellular-diagnostic-and-prognostic-biomarker-for-hepatocellular-carcinoma
#15
Luigi Buonaguro
No abstract text is available yet for this article.
December 2014: Hepatobiliary Surgery and Nutrition
https://www.readbyqxmd.com/read/25202690/tie2-expressing-monocytes-as-a-diagnostic-marker-for-hepatocellular-carcinoma-correlates-with-angiogenesis
#16
Domenico Germano, Bruno Daniele
Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related deaths worldwide. In the past few years, the mechanisms of hepato-carcinogenesis have been elucidated and the involvement of a number of pathways, including angiogenesis, aberrant signal transduction, and dysregulated cell cycle control have been demonstrated. Myeloid lineage cells, such as macrophages and monocytes, have been reported to regulate angiogenesis in mouse models. TIE2, a receptor of angiopoietins, conveys pro-angiogenic signals and identifies a monocyte/macrophage subset with pro-angiogenic activity...
August 2014: Hepatobiliary Surgery and Nutrition
https://www.readbyqxmd.com/read/25176420/tie2-dependent-deletion-of-%C3%AE-6-integrin-subunit-in-mice-reduces-tumor-growth-and-angiogenesis
#17
Claire Bouvard, Zacharie Segaoula, Adèle De Arcangelis, Isabelle Galy-Fauroux, Laetitia Mauge, Anne-Marie Fischer, Elisabeth Georges-Labouesse, Dominique Helley
The α6 integrin subunit (α6) has been implicated in cancer cell migration and in the progression of several malignancies, but its role in tumor angiogenesis is unclear. In mice, anti-α6 blocking antibodies reduce tumor angiogenesis, whereas Tie1-dependent α6 gene deletion enhances neovessel formation in melanoma and lung carcinoma. To clarify the discrepancy in these results we used the cre-lox system to generate a mouse line, α6fl/fl‑Tie2Cre(+), with α6 gene deletion specifically in Tie2-lineage cells: endothelial cells, pericytes, subsets of hematopoietic stem cells, and Tie2-expressing monocytes/macrophages (TEMs), known for their proangiogenic properties...
November 2014: International Journal of Oncology
https://www.readbyqxmd.com/read/24892425/macrophage-colony-stimulating-factor-augments-tie2-expressing-monocyte-differentiation-angiogenic-function-and-recruitment-in-a-mouse-model-of-breast-cancer
#18
Mary A Forget, Jeffrey L Voorhees, Sara L Cole, Duaa Dakhlallah, Ivory L Patterson, Amy C Gross, Leni Moldovan, Xiaokui Mo, Randall Evans, Clay B Marsh, Tim D Eubank
Reports demonstrate the role of M-CSF (CSF1) in tumor progression in mouse models as well as the prognostic value of macrophage numbers in breast cancer patients. Recently, a subset of CD14+ monocytes expressing the Tie2 receptor, once thought to be predominantly expressed on endothelial cells, has been characterized. We hypothesized that increased levels of CSF1 in breast tumors can regulate differentiation of Tie2- monocytes to a Tie2+ phenotype. We treated CD14+ human monocytes with CSF1 and found a significant increase in CD14+/Tie2+ positivity...
2014: PloS One
https://www.readbyqxmd.com/read/24809734/anti-vascular-endothelial-growth-factor-therapy-induced-glioma-invasion-is-associated-with-accumulation-of-tie2-expressing-monocytes
#19
Konrad Gabrusiewicz, Dan Liu, Nahir Cortes-Santiago, Mohammad B Hossain, Charles A Conrad, Kenneth D Aldape, Gregory N Fuller, Frank C Marini, Marta M Alonso, Miguel Angel Idoate, Mark R Gilbert, Juan Fueyo, Candelaria Gomez-Manzano
The addition of anti-angiogenic therapy to the few treatments available to patients with malignant gliomas was based on the fact that these tumors are highly vascularized and on encouraging results from preclinical and clinical studies. However, tumors that initially respond to this therapy invariably recur with the acquisition of a highly aggressive and invasive phenotype. Although several myeloid populations have been associated to this pattern of recurrence, a specific targetable population has not been yet identified...
April 30, 2014: Oncotarget
https://www.readbyqxmd.com/read/24416337/sirtuin1-over-expression-does-not-impact-retinal-vascular-and-neuronal-degeneration-in-a-mouse-model-of-oxygen-induced-retinopathy
#20
Shaday Michan, Aimee M Juan, Christian G Hurst, Zhenghao Cui, Lucy P Evans, Colman J Hatton, Dorothy T Pei, Meihua Ju, David A Sinclair, Lois E H Smith, Jing Chen
Proliferative retinopathy is a leading cause of blindness, including retinopathy of prematurity (ROP) in children and diabetic retinopathy in adults. Retinopathy is characterized by an initial phase of vessel loss, leading to tissue ischemia and hypoxia, followed by sight threatening pathologic neovascularization in the second phase. Previously we found that Sirtuin1 (Sirt1), a metabolically dependent protein deacetylase, regulates vascular regeneration in a mouse model of oxygen-induced proliferative retinopathy (OIR), as neuronal depletion of Sirt1 in retina worsens retinopathy...
2014: PloS One
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