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https://www.readbyqxmd.com/read/29453855/nerve-reinnervation-and-itch-behaviour-in-a-rat-burn-wound-model
#1
Tiam M Saffari, Barthold N Schüttenhelm, Johan W van Neck, Jan C Holstege
In this study, we investigated whether post-burn itch in rats, after a full thickness burn, is correlated to the nervous reinnervation of the burn wound area. For this purpose, we determined scratching duration (expressed as sec/hr) at 24 hours, 2, 4, 8 and 12 weeks post-burn and combined this with immunohistochemistry for protein gene product 9.5 (PGP9.5) to identify all nerve fibers, calcitonin gene related peptide (CGRP) to identify peptidergic fibers, tyrosine hydroxylase (TH) for sympathetic fibers and growth-associated protein 43 (GAP-43) for regrowing fibers...
February 17, 2018: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29451771/structural-transformation-of-wireframe-dna-origami-via-dna-polymerase-assisted-gap-filling
#2
Nayan P Agarwal, Michael Matthies, Bastian Joffroy, Thorsten L Schmidt
The programmability of DNA enables constructing nanostructures with almost any arbitrary shape, which can be decorated with many functional materials. Moreover, dynamic structures can be realized such as molecular motors and walkers. In this work, we have explored the possibility to synthesize the complementary sequences to single-stranded gap regions in the DNA origami scaffold cost effectively by a DNA polymerase rather than by a DNA synthesizer. For this purpose, four different wireframe DNA origami structures were designed to have single-stranded gap regions...
February 16, 2018: ACS Nano
https://www.readbyqxmd.com/read/29451687/gpcrs-in-context-sexual-dimorphism-in-the-cardiovascular-system
#3
REVIEW
Margaret A Mouat, James L J Coleman, Nicola J Smith
Cardiovascular disease remains the largest cause of mortality worldwide and there is a clear gender gap in disease occurrence: men are predisposed to earlier onset of cardiovascular disease, including atherosclerosis and hypertension, than are women. Oestrogen may be a driving factor for female-specific cardioprotection, though androgens and sex chromosomes are also likely to contribute to sexual dimorphism in the cardiovascular system. Many G protein-coupled receptor (GPCR)-mediated processes are involved in cardiovascular homeostasis and some exhibit clear sex-divergence...
February 16, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29450777/recovery-and-characterization-of-proteins-from-pangas-pangasius-pangasius-processing-waste-obtained-through-ph-shift-processing
#4
Vijay Kumar Reddy Surasani, Tanaji Kudre, Rajashekhar V Ballari
Study was conducted to recover proteins from pangas (Pangasius pangasius) processing waste (fillet frames) using pH shift method and to characterize the recovered isolates. pH 2.0 from acidic range and pH 13.0 from alkaline range were found to have maximum protein recovery (p < 0.05). During the recovery process, acidic pH (pH 2.0) was found to have minimal effect on proteins resulting in more stable isolates and strong protein gels. Alkaline pH (pH 13.0) caused protein denaturation resulting in less stable proteins and poor gel network...
February 15, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29449883/production-of-2r-3r-2-3-butanediol-using-engineered-pichia-pastoris-strain-construction-characterization-and-fermentation
#5
Zhiliang Yang, Zisheng Zhang
Background: 2,3-butanediol (2,3-BD) is a bulk platform chemical with various potential applications such as aviation fuel. 2,3-BD has three optical isomers: (2R, 3R)-, (2S, 3S)- and meso-2,3-BD. Optically pure 2,3-BD is a crucial precursor for the chiral synthesis and it can also be used as anti-freeze agent due to its low freezing point. 2,3-BD has been produced in both native and non-native hosts. Several pathogenic bacteria were reported to produce 2,3-BD in mixture of its optical isomers including Klebsiella pneumoniae and Klebsiella oxytoca ...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29449773/potential-breast-anticancer-drug-targets-revealed-by-differential-gene-regulatory-network-analysis-and-molecular-docking-neoadjuvant-docetaxel-drug-as-a-case-study
#6
Adel Aloraini, Karim M ElSawy
Understanding gene-gene interaction and its causal relationship to protein-protein interaction is a viable route for understanding drug action at the genetic level, which is largely hindered by inability to robustly map gene regulatory networks. Here, we use biological prior knowledge of family-to-family gene interactions available in the KEGG database to reveal individual gene-to-gene interaction networks that underlie the gene expression profiles of 2 cell line data sets, sensitive and resistive to neoadjuvant docetaxel breast anticancer drug...
2018: Cancer Informatics
https://www.readbyqxmd.com/read/29449694/interaction-between-stat3-and-gli1-tgli1-oncogenic-transcription-factors-promotes-the-aggressiveness-of-triple-negative-breast-cancers-and-her2-enriched-breast-cancer
#7
Sherona R Sirkisoon, Richard L Carpenter, Tadas Rimkus, Ashley Anderson, Alexandria Harrison, Allison M Lange, Guangxu Jin, Kounosuke Watabe, Hui-Wen Lo
Signal transducer and activator of transcription 3 (STAT3), glioma oncogene homolog 1 (GLI1), and truncated GLI1 (tGLI1) are oncogenic transcription factors playing important roles in breast cancer. tGLI1 is a gain-of-function GLI1 isoform. Whether STAT3 physically and/or functionally interacts with GLI1/tGLI1 has not been explored. To address this knowledge gap, we analyzed 47 node-positive breast cancer specimens using immunohistochemical staining and found that phosphorylated-STAT3 (Y705), GLI1, and tGLI1 are co-overexpressed in the majority of triple-negative breast carcinomas (64%) and HER2-enriched (68%) breast carcinomas, and in lymph node metastases (65%)...
February 16, 2018: Oncogene
https://www.readbyqxmd.com/read/29448386/theoretical-analysis-of-the-distribution-of-isolated-particles-in-totally-asymmetric-exclusion-processes-application-to-mrna-translation-rate-estimation
#8
Khanh Dao Duc, Zain H Saleem, Yun S Song
The Totally Asymmetric Exclusion Process (TASEP) is a classical stochastic model for describing the transport of interacting particles, such as ribosomes moving along the messenger ribonucleic acid (mRNA) during translation. Although this model has been widely studied in the past, the extent of collision between particles and the average distance between a particle to its nearest neighbor have not been quantified explicitly. We provide here a theoretical analysis of such quantities via the distribution of isolated particles...
January 2018: Physical Review. E
https://www.readbyqxmd.com/read/29437274/hybrid-indicators-for-fast-and-sensitive-voltage-imaging
#9
Yongxian Xu, Luxin Peng, Sicong Wang, Anqi Wang, Ruirui Ma, Ying Zhou, Jiahe Yang, De-En Sun, Wei Lin, Xing Chen, Peng Zou
Membrane voltage is an important biophysical signal that underlies intercellular electrical communications. In this study, we present a novel fluorescent voltage indicator that enables the investigation of electrical signaling at high spatial resolution. Our method is built upon the site-specific modification of microbial rhodopsin proteins with organic fluorophores, resulting in a hybrid indicator scaffold that represents one of the most sensitive and fastest orange-colored voltage indicators developed to date...
February 13, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29434716/%C3%AE-thalassaemia-combined-with-hereditary-spherocytosis-in-the-same-patient
#10
Xiaohong Li, Lin Liao, Xuelian Deng, Jian Huang, Zengfu Deng, Hongying Wei, Wuning Mo, Faquan Lin
A family of four from the Guangxi Zhuang Autonomous Region of China, including a child with α-thalassaemia and hereditary spherocytosis (HS), underwent laboratory identification, and genetic analysis. After harvesting peripheral blood samples from the child patient and his family members, GAP-polymerase chain reaction (PCR) and reverse dot-blot tests were used to identify thalassaemia genotypes. After amplifying exons and the adjacent introns of solute carrier family 4 member 1 (Diego blood group) (SLC4A1), ankyrin 1, spectrin α erythrocytic 1, spectrin β erythrocytic and erythrocyte membrane protein band 4...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29434248/modulation-of-small-gtpase-activity-by-nme-proteins
#11
REVIEW
Vedrana Filić, Maja Marinović, Marko Šoštar, Igor Weber
NME proteins are reported to influence signal transduction activity of small GTPases from the Ras superfamily by diverse mechanisms in addition to their generic NDP kinase activity, which replenishes the cytoplasmic pool of GTP. Comprehensive evidence shows that NME proteins modulate the activity of Ras GTPases, in particular members of the Rho family, via binding to their major activators GEFs. Direct interaction between several NMEs and Ras GTPases were also indicated in vitro and in vivo. These modes of regulation are mainly independent of the NME's kinase activity...
February 12, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29434075/a-missense-mutation-in-gja8-encoding-connexin-50-in-a-chinese-pedigree-with-autosomal-dominant-congenital-cataract
#12
Lusi Zhang, Youling Liang, Yedi Zhou, Huilan Zeng, Songbai Jia, Jingming Shi
Congenital cataract is leading cause of visual impairment and blindness in children worldwide. Approximately one-third of congenital cataract cases are familial, whose genetic etiology can be distinguished by targeted exome sequencing. Here, a three-generation congenital cataract pedigree was recruited, and physical and ophthalmologic examinations were taken. Targeted exome sequencing of 139 cataract-related genes was performed on the proband III:1. Sanger sequencing was used to validate the presence of variation identified via exome sequencing in family members and 200 controls...
2018: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29428590/quantitative-and-ultrasensitive-detection-of-multiplex-cardiac-biomarkers-in-lateral-flow-assay-with-core-shell-sers-nanotags
#13
Di Zhang, Li Huang, Bing Liu, Haibin Ni, Liangdong Sun, Enben Su, Hongyuan Chen, Zhongze Gu, Xiangwei Zhao
Rapid and sensitive quantification of multiplex proteins in a wide concentration range is challenging in high throughput analysis. Herein, we proposed a lateral flow assay (LFA) based on core-shell surface enhanced Raman scattering (SERS) nanotags for multiplex and quantitative detection of cardiac biomarkers for the early diagnosis of acute myocardial infarction (AMI). In practice, Raman dyes (RDs) were embedded into the interior-gap of silver core and gold shell nanoparticles (NPs) to form SERS nanotags as labels instead of gold colloids and three test lines were employed in the strip for the detection of three cardiac biomarkers, Myo, cTnI, and CK-MB, respectively...
February 1, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29428206/proprotein-convertase-subtilisin-kexin-type-9-pcsk9-and-triglyceride-rich-lipoprotein-metabolism-facts-and-gaps
#14
REVIEW
Andrea Baragetti, Daniela Grejtakova, Manuela Casula, Elena Olmastroni, Gloria Saccani Jotti, Giuseppe Danilo Norata, Alberico L Catapano, Stefano Bellosta
After more than a decade of intense investigation, Pro-protein Convertase Subtilisin-Kexin type 9 (PCSK9) remains a hot topic of research both at experimental and clinical level. Interestingly PCSK9 is expressed in different tissues suggesting the existence of additional function(s) beyond the modulation of the Low-Density Lipoprotein (LDL) receptor in the liver. Emerging data suggest that PCSK9 might play a role in the modulation of triglyceride-rich lipoprotein (TGRL) metabolism, mainly Very Low-Density Lipoproteins (VLDL) and their remnants...
February 8, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29427253/lack-of-connexin-40-decreases-the-calcium-sensitivity-of-renin-secreting-juxtaglomerular-cells
#15
Dominik Steppan, Lisa Geis, Lin Pan, Kenneth Gross, Charlotte Wagner, Armin Kurtz
The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion...
February 9, 2018: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/29427105/spinocerebellar-ataxia-type-17-sca17
#16
Yasuko Toyoshima, Hitoshi Takahashi
In 1999, a polyglutamine expansion was identified in the transcription factor TATA-binding protein (TBP) in a patient with ataxia with negative family history. Subsequently, CAG/CAA repeat expansions in the TBP gene were identified in families with spinocerebellar ataxia (SCA), establishing this repeat expansion as the underlying mutation in SCA type 17 (SCA17). There are several characteristic differences between SCA17 and other polyglutamine diseases. First, SCA17 shows a complex and variable clinical phenotype, in some cases overlapping that of Huntington's disease...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29425753/effects-of-rhbmp-2-7-heterodimer-and-rada16-hydrogel-scaffold-on-bone-formation-during-rabbit-mandibular-distraction
#17
Ling-Fei Ren, Geng-Sheng Shi, Yong-Qing Tong, Song-Yao Jiang, Feng Zhang
PURPOSE: The effects of a recombinant human bone morphogenetic protein-2/7 (rhBMP-2/7) heterodimer and a RADA16 (Ac-RADARADARADARADA-CONH2) hydrogel scaffold on bone formation during distraction osteogenesis were evaluated. MATERIALS AND METHODS: Forty New Zealand white rabbits, which underwent mandibular lengthening, were randomly divided into 5 groups. One group served as the control group. The others received 2 μg of rhBMP-2 homodimer, 2 μg of rhBMP-2/7 heterodimer, 100 μL of RADA16, or 100 μL of RADA16 plus 2 μg of rhBMP-2/7 heterodimer in the mandibular distraction gap at the beginning of distraction...
January 31, 2018: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29424431/inhibitory-and-modulatory-inputs-to-the-vocal-central-pattern-generator-of-a-teleost-fish
#18
Elisabeth Rosner, Kevin N Rohmann, Andrew H Bass, Boris P Chagnaud
Vocalization is a behavioral feature that is shared among multiple vertebrate lineages, including fish. The temporal patterning of vocal communication signals is set, in part, by central pattern generators (CPGs). Toadfishes are well-established models for CPG coding of vocalization at the hindbrain level. The vocal CPG comprises three topographically separate nuclei: pre-pacemaker, pacemaker, motor. While the connectivity between these nuclei is well understood, their neurochemical profile remains largely unexplored...
February 9, 2018: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29423350/primary-psychiatric-disorder-masking-the-diagnosis-of-neuropsychiatric-lupus-in-a-patient-with-altered-mental-status-a-case-report
#19
Osman Perez, Kairavee Dave, Aimee Almanzar, Tajul Prodhan, Livasky Concepion
Neuropsychiatric systemic lupus erythematosus (NPSLE) has a wide variety of neurologic and psychiatric features. NPSLE symptoms and the psychotic features of primary psychiatric disorders often overlap with each other. These psychotic features often mask and delay the diagnosis of NPSLE. We present the case of a 59-year-old female previously diagnosed with bipolar disorder and generalized anxiety disorder presenting with altered mental status (AMS), subsequently diagnosed with neuropsychiatric lupus. Initially, medication overdose was suspected as an empty bottle of trazodone was found beside her...
October 23, 2017: Curēus
https://www.readbyqxmd.com/read/29423233/organellar-genome-assembly-methods-and-comparative-analysis-of-horticultural-plants
#20
Xuelin Wang, Feng Cheng, Dekai Rohlsen, Changwei Bi, Chunyan Wang, Yiqing Xu, Suyun Wei, Qiaolin Ye, Tongming Yin, Ning Ye
Although organellar genomes (including chloroplast and mitochondrial genomes) are smaller than nuclear genomes in size and gene number, organellar genomes are very important for the investigation of plant evolution and molecular ecology mechanisms. Few studies have focused on the organellar genomes of horticultural plants. Approximately 1193 chloroplast genomes and 199 mitochondrial genomes of land plants are available in the National Center for Biotechnology Information (NCBI), of which only 39 are from horticultural plants...
2018: Horticulture Research
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