keyword
MENU ▼
Read by QxMD icon Read
search

DRG neurons

keyword
https://www.readbyqxmd.com/read/28326931/overexpression-of-suppressor-of-cytokine-signaling-3-in-dorsal-root-ganglion-attenuates-cancer-induced-pain-in-rats
#1
Jinrong Wei, Meng Li, Dieyu Wang, Hongyan Zhu, Xiangpeng Kong, Shusheng Wang, You-Lang Zhou, Zhong Ju, Guang-Yin Xu, Guo-Qin Jiang
Background Cancer-induced pain (CIP) is one of the most severe types of chronic pain with which clinical treatment remains challenging and the involved mechanisms are largely unknown. Suppressor of cytokine signaling 3 (SOCS3) is an important intracellular protein and provides a classical negative feedback loop, thus involving in a wide variety of processes including inflammation and nociception. However, the role of SOCS3 pathway in CIP is poorly understood. The present study was designed to investigate the role of SOCS3 in dorsal root ganglion (DRG) in the development of CIP...
January 2017: Molecular Pain
https://www.readbyqxmd.com/read/28326927/glial-activation-in-the-collagenase-model-of-nociception-associated-with-osteoarthritis
#2
Sara Adães, Lígia Almeida, Catarina S Potes, Ana Rita Ferreira, José M Castro-Lopes, Joana Ferreira-Gomes, Fani L Neto
Background Experimental osteoarthritis entails neuropathic-like changes in dorsal root ganglia (DRG) neurons. Since glial activation has emerged as a key player in nociception, being reported in numerous models of neuropathic pain, we aimed at evaluating if glial cell activation may also occur in the DRG and spinal cord of rats with osteoarthritis induced by intra-articular injection of collagenase. Methods Osteoarthritis was induced by two injections, separated by three days, of 500 U of type II collagenase into the knee joint of rats...
January 2017: Molecular Pain
https://www.readbyqxmd.com/read/28326469/hsv-1-interaction-to-3-o-sulfated-heparan-sulfate-in-mouse-derived-drg-explant-and-profiles-of-inflammatory-markers-during-virus-infection
#3
Harsh Sharthiya, Chanmoly Seng, T H Van Kuppevelt, Vaibhav Tiwari, Michele Fornaro
The molecular mechanism of herpes simplex virus (HSV) entry and the associated inflammatory response in the nervous system remain poorly understood. Using mouse-derived ex vivo dorsal root ganglia (DRG) explant model and single cell neurons (SCNs), in this study, we provided a visual evidence for the expression of heparan sulfate (HS) and 3-O-sulfated heparan sulfate (3-OS HS) followed by their interactions with HSV-1 glycoprotein B (gB) and glycoprotein D (gD) during cell entry. Upon heparanase treatment of DRG-derived SCN, a significant inhibition of HSV-1 entry was observed suggesting the involvement of HS role during viral entry...
March 21, 2017: Journal of Neurovirology
https://www.readbyqxmd.com/read/28322749/cannabinoid-receptor-1-contributes-to-sprouted-innervation-in-endometrial-ectopic-growth-through-mitogen-activated-protein-kinase-activation
#4
Hongxiu Han, Xizi Liang, Juan Wang, Qianqian Zhao, Mei Yang, Weifang Rong, Guohua Zhang
The endocannabinoid system regulates neurite outgrowth and neurogenesis during development of the central nervous system. Cannabinoid receptor 1 (CB1R) is expressed in neurons, including the somata and fibers, that innervate the endometrial ectopic cyst in rats. Here, we investigated the contribution of CB1R and its downstream signaling to the innervation of endometrial ectopic growth. We found that intrathecal injection of a CB1R agonist enhanced both the density of protein gene product (PGP) 9.5-immunoreactive sprouted nerve fibers and the protein level of PGP 9...
March 17, 2017: Brain Research
https://www.readbyqxmd.com/read/28315477/magnetic-but-not-non-magnetic-reduced-graphene-oxide-in-spinal-cord-increases-nociceptive-neuronal-responsiveness
#5
Luis Paulo Manzo, Helder Ceragioli, Ivan José Bonet, Catarine Massucato Nishijima, Willians Fernando Vieira, Elaine Conceição Oliveira, João-Batista Destro-Filho, César Renato Sartori, Claudia Herrera Tambeli, Carlos Amilcar Parada
The interference between external magnetic fields and neurophysiology is not new, however, the role of the neuronal magnetic field remains unclear. This study aimed at investigating a possible role of the neuronal magnetic field in nociception. Highly and poorly magnetic reduced graphene oxide (rGO) were injected intrathecally in rats. Nociceptive responsiveness was greater in rats that received highly magnetic-rGO in von Frey electronic or intraplantar capsaicin tests. Furthermore, in vitro experiments demonstrated that the number of KCl-responsive DRG-neurons was greater when treated with highly magnetic-rGO when compared with non-magnetic-rGO...
March 14, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28286117/carvedilol-prevents-functional-deficits-in-peripheral-nerve-mitochondria-of-rats-with-oxaliplatin-evoked-painful-peripheral-neuropathy
#6
Aparna Areti, Prashanth Komirishetty, Ashutosh Kumar
Oxaliplatin use as chemotherapeutic agent is frequently limited by cumulative neurotoxicity which may compromise quality of life. Reports relate this neurotoxic effect to oxidative stress and mitochondrial dysfunction in peripheral nerves and dorsal root ganglion (DRG). Carvedilol is an antihypertensive drug, has also been appreciated for its antioxidant and mitoprotective properties. Carvedilol co-treatment did not reduce the anti-tumor effects of oxaliplatin in human colon cancer cells (HT-29), but exhibited free radical scavenging activity against oxaliplatin-induced oxidative stress in neuronal cells (Neuro-2a)...
March 9, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28281561/ep2-receptor-antagonism-reduces-peripheral-and-central-hyperalgesia-in-a-preclinical-mouse-model-of-endometriosis
#7
Erin Greaves, Andrew W Horne, Helen Jerina, Marta Mikolajczak, Lisa Hilferty, Rory Mitchell, Sue M Fleetwood-Walker, Philippa T K Saunders
Endometriosis is an incurable gynecological disorder characterized by debilitating pain and the establishment of innervated endometriosis lesions outside the uterus. In a preclinical mouse model of endometriosis we demonstrated overexpression of the PGE2-signaling pathway (including COX-2, EP2, EP4) in endometriosis lesions, dorsal root ganglia (DRG), spinal cord, thalamus and forebrain. TRPV1, a PGE2-regulated channel in nociceptive neurons was also increased in the DRG. These findings support the concept that an amplification process occurs along the pain neuroaxis in endometriosis...
March 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28276837/5-hydroxymethylcytosine-5hmc-and-ten-eleven-translocation-1-3-tet1-3-proteins-in-the-dorsal-root-ganglia-of-mouse-expression-and-dynamic-regulation-in-neuropathic-pain
#8
Alexander G Chamessian, Yawar J Qadri, Matthew Cummins, Michele Hendrickson, Temugin Berta, Thomas Buchheit, Thomas Van de Ven
Epigenetic mechanisms are increasingly implicated in chronic pain pathology. In this study, we demonstrate that the novel epigenetic mark 5-hydroxymethylcytosine (5hmC) is present in dorsal root ganglia (DRG) neurons and glia, and its levels increase following nerve injury. Furthermore, we show that the 5hmC-generating Ten-eleven translocation 1-3 (TET1-3) proteins are expressed in a cell-type specific manner in the DRG, with Tet3 displaying differential upregulation after injury, suggesting a potential role in neuropathic pain...
March 1, 2017: Somatosensory & Motor Research
https://www.readbyqxmd.com/read/28270793/expression-of-semaphorins-neuropilins-vegf-and-tenascins-in-rat-and-human-primary-sensory-neurons-after-a-dorsal-root-injury
#9
Tomas Lindholm, Mårten Risling, Thomas Carlstedt, Henrik Hammarberg, Wilhelm Wallquist, Staffan Cullheim, Mattias K Sköld
Dorsal root injury is a situation not expected to be followed by a strong regenerative growth, or growth of the injured axon into the central nervous system of the spinal cord, if the central axon of the dorsal root is injured but of strong regeneration if subjected to injury to the peripherally projecting axons. The clinical consequence of axonal injury is loss of sensation and may also lead to neuropathic pain. In this study, we have used in situ hybridization to examine the distribution of mRNAs for the neural guidance molecules semaphorin 3A (SEMA3A), semaphorin 3F (SEMA3F), and semaphorin 4F (SEMA4F), their receptors neuropilin 1 (NP1) and neuropilin 2 (NP2) but also for the neuropilin ligand vascular endothelial growth factor (VEGF) and Tenascin J1, an extracellular matrix molecule involved in axonal guidance, in rat dorsal root ganglia (DRG) after a unilateral dorsal rhizotomy (DRT) or sciatic nerve transcetion (SNT)...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28270689/dna-methyltransferase-dnmt3a-contributes-to-neuropathic-pain-by-repressing-kcna2-in-primary-afferent-neurons
#10
Jian-Yuan Zhao, Lingli Liang, Xiyao Gu, Zhisong Li, Shaogen Wu, Linlin Sun, Fidelis E Atianjoh, Jian Feng, Kai Mo, Shushan Jia, Brianna Marie Lutz, Alex Bekker, Eric J Nestler, Yuan-Xiang Tao
Nerve injury induces changes in gene transcription in dorsal root ganglion (DRG) neurons, which may contribute to nerve injury-induced neuropathic pain. DNA methylation represses gene expression. Here, we report that peripheral nerve injury increases expression of the DNA methyltransferase DNMT3a in the injured DRG neurons via the activation of the transcription factor octamer transcription factor 1. Blocking this increase prevents nerve injury-induced methylation of the voltage-dependent potassium (Kv) channel subunit Kcna2 promoter region and rescues Kcna2 expression in the injured DRG and attenuates neuropathic pain...
March 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28267064/nerve-injury-induced-epigenetic-silencing-of-opioid-receptors-controlled-by-dnmt3a-in-primary-afferent-neurons
#11
Linlin Sun, Jian-Yuan Zhao, Xiyao Gu, Lingli Liang, Shaogen Wu, Kai Mo, Jian Feng, Weixiang Guo, Jun Zhang, Alex Bekker, Xinyu Zhao, Eric J Nestler, Yuan-Xiang Tao
Opioids are the gold standard for pharmacological treatment of neuropathic pain, but their analgesic effects are unsatisfactory in part due to nerve injury-induced downregulation of opioid receptors in dorsal root ganglia (DRG) neurons. How nerve injury drives such downregulation remains elusive. DNA methyltransferase-(DNMT-) triggered DNA methylation represses gene expression. We show here that blocking the nerve injury-induced increase in DRG DNMT3a (a de novo DNMT) rescued the expression of Oprm1 and Oprk1 mRNAs and their respective encoding mu opioid receptor (MOR) and kappa opioid receptor (KOR) proteins in the injured DRG...
March 4, 2017: Pain
https://www.readbyqxmd.com/read/28263785/nociceptive-neuronal-fc-gamma-receptor-i-is-involved-in-igg-immune-complex-induced-pain-in-the-rat
#12
Haowu Jiang, Xinhua Shen, Zhiyong Chen, Fan Liu, Tao Wang, Yikuan Xie, Chao Ma
Antigen-specific immune diseases such as rheumatoid arthritis are often accompanied by pain and hyperalgesia. Our previous studies have demonstrated that Fc-gamma-receptor type I (FcγRI) is expressed in a subpopulation of rat dorsal root ganglion (DRG) neurons and can be directly activated by IgG immune complex (IgG-IC). In this study we investigated whether neuronal FcγRI contributes to antigen-specific pain in the naïve and rheumatoid arthritis model rats. In vitro calcium imaging and whole-cell patch clamp recordings in dissociated DRG neurons revealed that only the small-, but not medium- or large-sized DRG neurons responded to IgG-IC...
March 2, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28260107/cytoprotective-effects-of-myristicin-against-hypoxia%C3%A2-induced-apoptosis-and-endoplasmic-reticulum-stress-in-rat-dorsal-root-ganglion-neurons
#13
Quanlai Zhao, Chen Liu, Xiang Shen, Liang Xiao, Hong Wang, Ping Liu, Lingting Wang, Hongguang Xu
The aim of the present study was to investigate the role of myristicin (Myr; 1‑allyl‑5‑methoxy‑3,4‑methylenedioxybenzene), an active aromatic compound isolated from nutmeg, carrot, basil, cinnamon and parsley, in hypoxia‑induced apoptosis in rat dorsal root ganglion (DRG) neurons. It was observed that Myr significantly enhanced cell viability in hypoxia‑induced DRG neurons in a dose‑dependent manner; the optimal concentration of Myr was 50 µM. Furthermore, Myr reduced the percentage of deoxynucleotidyl transferase‑mediated dUTP nick end‑labeling‑positive neuronal cells and influenced the expression of the pro‑apoptotic gene B‑cell lymphoma 2 (Bcl‑2) associated X protein, the apoptosis protease cleaved caspase‑3 and the anti‑apoptotic gene Bcl‑2, in the hypoxia‑induced group...
February 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28258808/alendronate-inhibits-hyperalgesia-and-suppresses-neuropeptide-markers-of-pain-in-a-mouse-model-of-osteoporosis
#14
Yohei Naito, Hiroki Wakabayashi, Sho Kato, Taro Nakagawa, Takahiro Iino, Akihiro Sudo
BACKGROUND: Chronic back pain is one of the most important complications of postmenopausal osteoporosis. The aim of this study was to evaluate skeletal pain associated with osteoporosis and to examine the inhibitory effect of bisphosphonates (BPs) on pain in ovariectomized (OVX) mice. The mechanism of osteoporotic pain in OVX mice was evaluated through an examination of pain-related behavior, as well as immunohistochemical findings. In addition, the effects of alendronate (ALN), a potent osteoclast inhibitor, on these parameters were assessed...
February 28, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/28254884/a-functional-coupling-between-crmp1-and-nav1-7-for-retrograde-propagation-of-semaphorin3a-signaling
#15
Masayuki Yamane, Naoya Yamashita, Tomonobu Hida, Yoshinori Kamiya, Fumio Nakamura, Pappachan Kolattukudy, Yoshio Goshima
Semaphorin3A (Sema3A) is a secreted type of axon guidance molecules that regulates axon wiring through neuropilin-1 (NRP1) and PlexinAs (PlexAs) receptor complex. Sema3A regulates the dendritic branching through a tetrodotoxin (TTX)-sensitive retrograde axonal transport of PlexAs and Tropomyosin-related kinase A (TrkA) complex. We here demonstrate that Nav1.7, a TTX-sensitive Na(+) channel, by coupling with collapsin response mediator protein 1 (CRMP1), mediates the Sema3A-induced retrograde transport. In mouse dorsal root ganglion (DRG) neurons, Sema3A increased co-localization of PlexA4 and TrkA in the growth cones and axons...
March 2, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28254863/bone-pain-induced-by-multiple-myeloma-is-reduced-by-targeting-v-atpase-and-asic3
#16
Masahiro Hiasa, Tatsuo Okui, Yohance M Allette, Matthew S Ripsch, Ge-Hong Sun-Wada, Hiroki Wakabayashi, G David Roodman, Fletcher A White, Toshiyuki Yoneda
Multiple myeloma patients experience severe bone pain (MMBP) that is undertreated and poorly understood. In this study, we studied MMBP in an intratibial mouse xenograft model that employs JJN3 human multiple myeloma cells. In this model, mice develop MMBP associated in bone with increased sprouting of calcitonin gene-related peptide-positive (CGRP(+)) sensory nerves and in dorsal root ganglia (DRG) with upregulation of phosphorylated ERK1/2 (pERK1/2) and pCREB, two molecular indicators of neuron excitation...
March 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28253350/sox10-contributes-to-the-balance-of-fate-choice-in-dorsal-root-ganglion-progenitors
#17
Mariana Delfino-Machín, Romain Madelaine, Giorgia Busolin, Masataka Nikaido, Sarah Colanesi, Karen Camargo-Sosa, Edward W P Law, Stefano Toppo, Patrick Blader, Natascia Tiso, Robert N Kelsh
The development of functional peripheral ganglia requires a balance of specification of both neuronal and glial components. In the developing dorsal root ganglia (DRGs), these components form from partially-restricted bipotent neuroglial precursors derived from the neural crest. Work in mouse and chick has identified several factors, including Delta/Notch signaling, required for specification of a balance of these components. We have previously shown in zebrafish that the Sry-related HMG domain transcription factor, Sox10, plays an unexpected, but crucial, role in sensory neuron fate specification in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28250634/sudden-death-and-cervical-spine-a-new-contribution-to-pathogenesis-for-sudden-death-in-critical-care-unit-from-subarachnoid-hemorrhage-first-report-an-experimental-study
#18
Hizir Kazdal, Ayhan Kanat, Mehmet Dumlu Aydin, Ugur Yazar, Ali Riza Guvercin, Muhammet Calik, Betul Gundogdu
CONTEXT: Sudden death from subarachnoid hemorrhage (SAH) is not uncommon. AIMS: The goal of this study is to elucidate the effect of the cervical spinal roots and the related dorsal root ganglions (DRGs) on cardiorespiratory arrest following SAH. SETTINGS AND DESIGN: This was an experimental study conducted on rabbits. MATERIALS AND METHODS: This study was conducted on 22 rabbits which were randomly divided into three groups: control (n = 5), physiologic serum saline (SS; n = 6), SAH groups (n = 11)...
January 2017: Journal of Craniovertebral Junction and Spine
https://www.readbyqxmd.com/read/28250148/mtor-regulates-peripheral-nerve-response-to-tensile-strain
#19
James M Love, Brian G Bober, Elisabeth Orozco, Amanda T White, Shannon N Bremner, Richard M Lovering, Simon Schenk, Sameer B Shah
While excessive tensile strain can be detrimental to nerve function, strain can be a positive regulator of neuronal outgrowth. We used an in vivo rat model of sciatic nerve strain to investigate signaling mechanisms underlying peripheral nerve response to deformation. Nerves were deformed by 11%, and did not demonstrate deficits in compound action potential latency or amplitude during or after 6 hours of strain. As revealed by Western blotting, application of strain resulted in significant upregulation of mammalian target of rapamycin (mTOR) and S6 signaling in nerves, increased myelin basic protein (MBP) and β-actin levels, and increased phosphorylation of neurofilament subunit H (NF-H) compared to unstrained (sham) contralateral nerves (p < 0...
March 1, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28250049/diabetic-polyneuropathy-sensory-neurons-nuclear-structure-and-spliceosome-alterations-a-role-for-cwc22
#20
Masaki Kobayashi, Ambika Chandrasekhar, Chu Cheng, Jose A Martinez, Hilarie Ng, Cristiane de la Hoz, Douglas W Zochodne
Unique deficits in the function of adult sensory neurons as part of their early neurodegeneration might account for progressive polyneuropathy during chronic diabetes mellitus. Here, we provide structural and functional evidence for aberrant pre-mRNA splicing in a chronic type 1 model of experimental diabetic polyneuropathy (DPN). Cajal bodies (CBs), unique nuclear substructures involved in RNA splicing, increased in number in diabetic sensory neurons, but their expected colocalization with survival motor neuron (SMN) proteins was reduced - a mislocalization described in motor neurons of spinal muscular atrophy...
March 1, 2017: Disease Models & Mechanisms
keyword
keyword
7991
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"