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https://www.readbyqxmd.com/read/29671154/cell-mimicking-microparticles-influence-the-organization-growth-and-mechanophenotype-of-stem-cell-spheroids
#1
Nicholas R Labriola, Jessica S Sadick, Jeffrey R Morgan, Edith Mathiowitz, Eric M Darling
Substrate stiffness is known to alter cell behavior and drive stem cell differentiation, though most research in this area has been restricted to traditional, two-dimensional culture systems rather than more physiologically relevant, three-dimensional (3D) platforms. In this study, we utilized polymer-based, cell mimicking microparticles (CMMPs) to deliver distinct, stable mechanical cues to human adipose derived stem cells in 3D spheroid culture to examine changes in adipogenic differentiation response and mechanophenotype...
April 18, 2018: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29665934/-effect-of-regulating-ppar%C3%AE-by-mtor-signaling-on-adipogenesis-of-bone-marrow-mesenchymal-stem-cells-from-aplastic-anemia
#2
Fang Wei, Wei-Huan Zhang, Xiu-Lian Zhang, Xiao-Jing Ge, Shu-Qin Zhang
OBJECTIVE: To study the effect and mechanism of mTOR signaling on adipogenesis of bone marrow mesenchymal stem cells(BM-MSCs) from aplastic anemia (AA) patients through regulation of PPARγ. METHODS: BM-MSCs were isolated from 24 newly diagnosed AA patients and 24 healthy controls. The surface antigen expression of BM-MSCs was identified by flow cytometry. The capacity of adipogenic differentiation of BM-MSCs was determined by lipid droplets based on Oil Red O staining and by the expression of FABP4 based on Western blot...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29658914/visualization-and-quantification-of-mesenchymal-cell-adipogenic-differentiation-potential-with-a-lineage-specific-marker
#3
Jennifer Eom, Vaughan Feisst, Louis Ranjard, Kerry Loomes, Tanvi Damani, Victoria Jackson-Patel, Michelle Locke, Hilary Sheppard, Pritika Narayan, P Rod Dunbar
Several dyes are currently available for use in detecting differentiation of mesenchymal cells into adipocytes. Dyes, such as Oil Red O, are cheap, easy to use and widely utilized by laboratories analyzing the adipogenic potential of mesenchymal cells. However, they are not specific to changes in gene transcription. We have developed a gene-specific differentiation assay to analyze when a mesenchymal cell has switched its fate to an adipogenic lineage. Immuno-labelling against fatty acid binding protein-4 (FABP4), a lineage-specific marker of adipogenic differentiation, enabled visualization and quantification of differentiated cells...
March 31, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29605806/actin-depolymerization-enhances-adipogenic-differentiation-in-human-stromal-stem-cells
#4
Li Chen, Huimin Hu, Weimin Qiu, Kaikai Shi, Moustapha Kassem
Human stromal stem cells (hMSCs) differentiate into adipocytes that play a role in skeletal tissue homeostasis and whole body energy metabolism. During adipocyte differentiation, hMSCs exhibit significant changes in cell morphology suggesting changes in cytoskeletal organization. Here, we examined the effect of direct modulation of actin microfilament dynamics on adipocyte differentiation. Stabilizing actin filaments in hMSCs by siRNA-mediated knock down of the two main actin depolymerizing factors (ADFs): Cofilin 1 (CFL1) and Destrin (DSTN) or treating the cells by Phalloidin reduced adipocyte differentiation as evidenced by decreased number of mature adipocytes and decreased adipocyte specific gene expression (ADIPOQ, LPL, PPARG, FABP4)...
March 18, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29604377/pomegranate-polyphenols-and-urolithin-a-inhibit-%C3%AE-glucosidase-dipeptidyl-peptidase-4-lipase-triglyceride-accumulation-and-adipogenesis-related-genes-in-3t3-l1-adipocyte-like-cells
#5
Francisco Les, José Miguel Arbonés-Mainar, Marta Sofía Valero, Víctor López
ETHNOPHARMACOLOGICAL RELEVANCE: pomegranate fruit is considered an antidiabetic medicine in certain systems of traditional medicine. In addition, pomegranate polyphenols are known as powerful antioxidants with beneficial effects such as the reduction of oxidative / inflammatory stress and the increase of protective signalling such as antioxidant enzymes, neurotrophic factors and cytoprotective proteins. AIM OF THE STUDY: this work evaluates the effects of pomegranate juice, its main polyphenols known as ellagic acid and punicalagin, as well as its main metabolite urolithin A, on physiological and pharmacological targets of metabolic diseases such as obesity and diabetes...
March 28, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29596447/citrus-aurantium-l-dry-extracts-promote-c-ebp%C3%AE-expression-and-improve-adipocyte-differentiation-in-3t3-l1-cells
#6
Gregory Alexander Raciti, Francesca Fiory, Michele Campitelli, Antonella Desiderio, Rosa Spinelli, Michele Longo, Cecilia Nigro, Giacomo Pepe, Eduardo Sommella, Pietro Campiglia, Pietro Formisano, Francesco Beguinot, Claudia Miele
Metabolic and/or endocrine dysfunction of the white adipose tissue (WAT) contribute to the development of metabolic disorders, such as Type 2 Diabetes (T2D). Therefore, the identification of products able to improve adipose tissue function represents a valuable strategy for the prevention and/or treatment of T2D. In the current study, we investigated the potential effects of dry extracts obtained from Citrus aurantium L. fruit juice (CAde) on the regulation of 3T3-L1 cells adipocyte differentiation and function in vitro...
2018: PloS One
https://www.readbyqxmd.com/read/29593201/adipose-tissue-critical-contributor-to-the-development-of-prostate-cancer
#7
Uehara Hisanori, Tomoko Kobayashi, Minoru Matsumoto, Shunsuke Watanabe, Akiko Yoneda, Bando Yoshimi
The prostate is surrounded by periprostatic adipose tissue. Although adipose tissue was thought to play limited physiological roles, it has recently been recognized as an active endocrine organ, secreting growth factors and adipokines. Epidemiologically, obesity is associated with prostate cancer progression. A major mechanism to explain the link between obesity and cancer includes the insulin and insulin-like growth factor (IGF)-1 axis, sex steroids, and adipokines. When prostate cancer cells invade periprostatic adipose tissue, adipose tissue contributes to create the tumor microenvironment, mainly via adipokine secretion...
2018: Journal of Medical Investigation: JMI
https://www.readbyqxmd.com/read/29572893/subchronic-cadmium-exposure-upregulates-the-mrna-level-of-genes-associated-to-hepatic-lipid-metabolism-in-adult-female-cd1-mice
#8
Jun Zhang, Yan Wang, Lin Fu, Yu-Jie Feng, Yan-Li Ji, Hua Wang, De-Xiang Xu
Cadmium (Cd) is a persistent environmental and occupational contaminant that accumulates in humans and shows adverse effects on health. Accumulating evidence reveals that environmental Cd exposure is associated with hepatic lipid accumulation and metabolic alterations in adult male mice. However, whether Cd exposure induces hepatic lipid accumulation and metabolic alterations in female mice remains poorly understood. In the present study, we aimed to investigate the effects of Cd exposure on insulin resistance, hepatic lipid accumulation and associated metabolic pathways...
March 24, 2018: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29568399/ctbp1-and-metabolic-syndrome-induce-an-mrna-and-mirna-expression-profile-critical-for-breast-cancer-progression-and-metastasis
#9
Paula L Farré, Georgina D Scalise, Rocío B Duca, Guillermo N Dalton, Cintia Massillo, Juliana Porretti, Karen Graña, Kevin Gardner, Paola De Luca, Adriana De Siervi
Metastatic breast cancer (BrCa) is still one of the main causes of cancer death in women. Metabolic syndrome (MeS), a risk factor for BrCa, is associated to high grade tumors, increased metastasis and recurrence of this disease. C-terminal binding protein 1 (CTBP1) is a co-repressor of tumor suppressor genes that is activated by low NAD+ /NADH ratio. Previously, we demonstrated that CTBP1 hyperactivation by MeS increased tumor growth in MDA-MB-231-derived xenografts regulating several genes and miRNAs. In this work, our aim was to elucidate the role of CTBP1 and MeS in BrCa metastasis...
March 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29559910/liposome-encapsulated-baicalein-suppressed-lipogenesis-and-extracellular-matrix-formation-in-hs68-human-dermal-fibroblasts
#10
Chien-Liang Fang, Yiwei Wang, Kevin H-Y Tsai, Hsin-I Chang
The dermis of human skin contains large numbers of fibroblasts that are responsible for the production of the extracellular matrix (ECM) that supporting skin integrity, elasticity and wound healing. Previously, an in vivo study demonstrated that dermal fibroblasts siting in the lower dermis are capable to convert into skin adipose layer and hence fibroblast lipogenesis may vary the structure and elasticity of dermis. In the present study, Hs68 human dermal fibroblasts were utilized as an in vitro model to study the lipogenesis via using adipogenic differentiation medium (ADM)...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29554135/thermogenic-capacity-of-human-periaortic-adipose-tissue-is-transformed-by-body-weight
#11
Diana Vargas, Carolina López, Edward Acero, Edgar Benitez, Angélica Wintaco, Jaime Camacho, Marisol Carreño, Juan Umaña, Daniela Jimenez, Said Díaz, Fernando Lizcano
The anatomical location of adipose tissue might have direct implications for its functionality and risk of cardiovascular disease. Adipose tissue surrounding blood vessels may be thermogenically more active in specific areas of the body, releasing substances that regulate vascular metabolism. In humans, the phenotypic characteristics of adipose tissue surrounding the aorta and the cardiovascular disease risk that it might entail remain largely unknown. Here, we compared thermogenesis-related molecular features of human periaortic adipose tissue samples with those of subcutaneous adipose tissue, obtained by sternotomy from 42 patients undergoing cardiovascular surgery...
2018: PloS One
https://www.readbyqxmd.com/read/29550588/fabp4-inhibitor-bms309403-decreases-saturated-fatty-acid-induced-endoplasmic-reticulum-stress-associated-inflammation-in-skeletal-muscle-by-reducing-p38-mapk-activation
#12
Alba Bosquet, Josefa Girona, Sandra Guaita-Esteruelas, Mercedes Heras, Paula Saavedra-García, Neus Martínez-Micaelo, Lluís Masana, Ricardo Rodríguez-Calvo
AIMS: Fatty acid binding protein 4 (FABP4) inhibitors have been proposed as potential therapeutic approaches against insulin resistance-related inflammation and type 2 diabetes mellitus. However, the underlying molecular mechanisms by which these molecules drive these effects in skeletal muscle remain unknown. Here, we assessed whether the FABP4 inhibitor BMS309403 prevented lipid-induced endoplasmic reticulum (ER) stress-associated inflammation in skeletal muscle. MATERIALS AND METHODS: The BMS309403 treatment was assessed both in the skeletal muscle of high-fat diet (HFD)-fed mice and in palmitate-stimulated C2C12 myotubes...
March 14, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29549054/knocking-out-or-pharmaceutical-inhibition-of-fatty-acid-binding-protein-4-fabp4-alleviates-osteoarthritis-induced-by-high-fat-diet-in-mice
#13
Chaofan Zhang, Kwong Yuen Chiu, Boris Pok Man Chan, Teng Li, Chunyi Wen, Aimin Xu, Chun Hoi Yan
OBJECTIVES: Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of FABP4 in OA. METHODS: Seventy-two FABP4 knockout mice (KO) in C57BL/6N background and wild-type littermates (WT) (male, 6-week-old) were fed with a high-fat diet (HFD, 60% calorie) or standard diet (STD, 11...
March 13, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29545239/senescence-alters-pparg-peroxisome-proliferator-activated-receptor-gamma-dependent-fatty-acid-handling-in-human-adipose-tissue-microvascular-endothelial-cells-and-favors-inflammation
#14
Anaïs Briot, Pauline Decaunes, Fanny Volat, Chloé Belles, Muriel Coupaye, Séverine Ledoux, Anne Bouloumié
OBJECTIVE: Adipose tissue (AT) dysfunction associated with obesity or aging is a major cause for lipid redistribution and the progression of cardiometabolic disorders. Our goal is to decipher the contribution of human AT microvascular endothelial cells (ECs) in the maintenance of fatty acid (FA) fluxes and the impact of senescence on their function. APPROACH AND RESULTS: We used freshly isolated primary microvascular ECs from human AT. Our data identified the endothelial FA handling machinery including FATPs (FA transport proteins) FATP1, FATP3, FATP4, and CD36 as well as FABP4 (FA binding protein 4)...
March 15, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29538677/critical-role-of-mtor-ppar%C3%AE-and-ppar%C3%AE-signaling-in-regulating-early-pregnancy-decidual-function-embryo-viability-and-feto-placental-growth
#15
Sabrina L Roberti, Romina Higa, Verónica White, Theresa L Powell, Thomas Jansson, Alicia Jawerbaum
STUDY QUESTION: What are the consequences of inhibiting mTOR, the mechanistic target of rapamycin, and the peroxisome proliferator activated receptor gamma (PPARγ) and PPARδ pathways in the early post-implantation period on decidual function, embryo viability and feto-placental growth in the rat? SUMMARY ANSWER: mTOR inhibition from day 7 to day 9 of pregnancy in rats caused decidual PPARγ and PPARδ upregulation on day 9 of pregnancy and resulted in embryo resorption by day 14 of pregnancy...
March 12, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29535811/thyroid-follicular-adenomas-and-carcinomas-molecular-profiling-provides-evidence-for-a-continuous-evolution
#16
Geneviève Dom, Sandra Frank, Sebastien Floor, Pashalina Kehagias, Frederick Libert, Catherine Hoang, Guy Andry, Alex Spinette, Ligia Craciun, Nicolas de Saint Aubin, Christophe Tresallet, Frederique Tissier, Frederique Savagner, Samira Majjaj, Ilse Gutierrez-Roelens, Etienne Marbaix, Jacques E Dumont, Carine Maenhaut
Non-autonomous thyroid nodules are common in the general population with a proportion found to be cancerous. A current challenge in the field is to be able to distinguish benign adenoma (FA) from preoperatively malignant thyroid follicular carcinoma (FTC), which are very similar both histologically and genetically. One controversial issue, which is currently not understood, is whether both tumor types represent different molecular entities or rather a biological continuum. To gain a better insight into FA and FTC tumorigenesis, we defined their molecular profiles by mRNA and miRNA microarray...
February 13, 2018: Oncotarget
https://www.readbyqxmd.com/read/29534587/an-extensive-study-of-the-mechanism-of-prostate-cancer-metastasis
#17
Y Wang, W Guo, H Xu, X Zhu, T Yu, Z Jiang, Q Jiang, X Gang
The study aimed to identify the pivotal genes and pathways involved in prostate cancer metastasis. Using the expression profile dataset GSE7930, downloaded from the Gene Expression Omnibus (GEO) database, differentially expressed genes (DEGs) between primary and highly metastatic prostate cell samples were screened, followed by functional analysis and tumor associated genes (TAG) screening. Protein-protein interaction (PPI) network of DEGs was constructed and module analysis was performed. The expression of DEGs and pathway related genes were evaluated by PCR analysis and the migra- tion ability of prostate tumor cells was observed after FABP4-siRNA blocking...
2018: Neoplasma
https://www.readbyqxmd.com/read/29526626/serum-concentrations-of-fatty-acid-binding-protein-4-in-chinese-children-with-type-1-diabetes-mellitus
#18
Huan Liu, Min Guo, Fu-Li Jiang
OBJECTIVE: The aim of this study was to test the serum concentrations of fatty acid-binding protein 4(FABP4) in children with type 1 diabetes mellitus (T1DM) and to determine the relationship between patients with good and poor glycaemic control who were classified according to their glycated hemoglobin A1c (HbA1c) levels. METHODS: Children with T1DM were selected consecutively from our department from May 2016 to May 2017. For comparison, the same non-diabetic, age, sex, BMI and pubertal stage-matched healthy children were selected consecutively among non-diabetic children...
December 28, 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/29515060/isolation-and-characterization-of-buffalo-bubalus-bubalis-amniotic-mesenchymal-stem-cells-derived-from-amnion-from-the-first-trimester-pregnancy
#19
Yanfei Deng, Guiting Huang, Lingxiu Zou, Tianying Nong, Xiaoling Yang, Jiayu Cui, Yingming Wei, Sufang Yang, Deshun Shi
Amniotic mesenchymal stem cells (AMSCs) from livestock are valuable resources for animal reproduction and veterinary therapeutic. The purpose of this study is to explore a suitable way to isolate and culture the buffalo AMSCs (bAMSCs), and to identify their biological characteristics. Digestion with a combination of trypsin-EDTA and collagenase type I could obtain pure bAMSCs more effectively than trypsin-EDTA or collagenase type I alone. bAMSCs could proliferate steadily in vitro culture and exhibited fibroblastic-like morphology in vortex-shaped colony...
March 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29511320/preadipocytes-from-obese-humans-with-type-2-diabetes-are-epigenetically-reprogrammed-at-genes-controlling-adipose-tissue-function
#20
Emil Andersen, Lars Roed Ingerslev, Odile Fabre, Ida Donkin, Ali Altıntaş, Soetkin Versteyhe, Thue Bisgaard, Viggo B Kristiansen, David Simar, Romain Barrès
BACKGROUND: Deterioration of the adipogenic potential of preadipocytes may contribute to adipose tissue dysfunction in obesity and type 2 diabetes (T2D). Here, we hypothesized that extracellular factors in obesity epigenetically reprogram adipogenesis potential and metabolic function of preadipocytes. METHODS: The transcriptomic profile of visceral adipose tissue preadipocytes collected from Lean, Obese and Obese with T2D was assessed throughout in vitro differentiation using RNA sequencing...
February 20, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
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