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https://www.readbyqxmd.com/read/28231414/mir-135a-3p-as-a-promising-biomarker-and-nucleic-acid-therapeutic-agent-for-ovarian-cancer
#1
Satoshi Fukagawa, Kohei Miyata, Fusanori Yotsumoto, Chihiro Kiyoshima, Sung Ouk Nam, Haruchika Anan, Takahiro Katsuda, Daisuke Miyahara, Masaharu Murata, Hiroshi Yagi, Kyoko Shirota, Shin'ichiro Yasunaga, Kiyoko Kato, Shingo Miyamoto
Ovarian cancer is the most lethal gynecologic malignancy. Recently, several molecularly targeted anticancer agents have been developed for ovarian cancer; however, its prognosis has remained extremely poor. The development of molecularly targeted therapy, as well as companion diagnostics, is required to improve outcomes for patients with ovarian cancer. In this study, to identify microRNAs involved in the progression of ovarian cancer we analyzed serum microRNAs in patients with ovarian cancer using microRNA array and qRT-PCR and examined the anticancer properties of microRNA expression in ovarian cancer cells...
February 23, 2017: Cancer Science
https://www.readbyqxmd.com/read/28220535/interaction-of-approved-drugs-with-synaptic-vesicle-protein-2a
#2
Azeem Danish, Vigneshwaran Namasivayam, Anke C Schiedel, Christa E Müller
Levetiracetam (LEV) and its recently approved derivative brivaracetam are anti-epileptic drugs with a unique mechanism of action. The synaptic vesicle protein 2A (SV2A) was previously identified as their main target. In the current study, we tested a collection of 500 approved drugs for interaction with the human SV2A protein expressed in Chinese hamster ovary cells. Competition binding studies were performed using cell lysates with high SV2A expression and [(3) H]brivaracetam as a radioligand. A hit rate of 3% was obtained, defined as compounds that inhibited radioligand binding by more than 90% at a screening concentration of 20 μM...
February 21, 2017: Archiv der Pharmazie
https://www.readbyqxmd.com/read/28219140/-effects-of-transient-exposure-to-high-glucose-on-biological-behaviors-of-human-dermal-microvascular-endothelial-cells
#3
L Qiao, H Z Yang, X C Li, X Q Huang, B Yuan, Z D Zhou
Objective: To observe the effects of transient exposure to high glucose on biological behaviors of human dermal microvascular endothelial cells cultured in vitro. Methods: The dividing method and treatment of cells for the detection of all indexes in this study were as follows. Human dermal microvascular endothelial cells of the 4th passage were divided into 3 groups according to the random number table, with 12 wells in each group. Cells in control group (C) were cultured with complete culture solution containing 5 mmol/L D-glucose for 7 d...
February 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28218815/glycoengineering-of-esterase-activity-through-metabolic-flux-based-modulation-of-sialic-acid
#4
Mohit Mathew, Elaine Tan, Jason W Labonte, Shivam Shah, Christopher T Saeui, Lingshu Liu, Rahul Bhattacharya, Patawut Bovonratwet, Jeffrey J Gray, Kevin Yarema
This report describes the metabolic glycoengineering (MGE) of intracellular esterase activity in human colon cancer (LS174T) and Chinese hamster ovary (CHO) cells. In silico analysis of the carboxylesterases CES1 and CES2 suggested that these enzymes are modified with sialylated N-glycans, which are proposed to stabilize the active multimeric forms of these enzymes. This premise was supported by treating cells with butanolylated ManNAc to increase sialylation, which in turn increased esterase activity. By contrast, hexosamine analogs not targeted to sialic acid biosynthesis (e...
February 20, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28216629/matrix-attachment-region-combinations-increase-transgene-expression-in-transfected-chinese-hamster-ovary-cells
#5
Chun-Peng Zhao, Xiao Guo, Si-Jia Chen, Chang-Zheng Li, Yun Yang, Jun-He Zhang, Shao-Nan Chen, Yan-Long Jia, Tian-Yun Wang
Matrix attachment regions (MARs) are cis-acting DNA elements that can increase transgene expression levels in a CHO cell expression system. To investigate the effects of MAR combinations on transgene expression and the underlying regulatory mechanisms, we generated constructs in which the enhanced green fluorescent protein (eGFP) gene flanked by different combinations of human β-interferon and β-globin MAR (iMAR and gMAR, respectively), which was driven by the cytomegalovirus (CMV) or simian virus (SV) 40 promoter...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28216407/multidrug-resistance-transporter-1-and-breast-cancer-resistance-protein-protect-against-ovarian-toxicity-and-are-essential-in-ovarian-physiology
#6
Lynae M Brayboy, Nathalie Oulhen, Sokunvichet Long, Niesha Voigt, Christina Raker, Gary M Wessel
Ovarian protection from chemotoxicity is essential for reproductive health. Our objective is to determine the role of ATP-dependent, Multidrug Resistance Transporters (MDRs) in this protection. Previously we identified MDR-dependent cytoprotection from cyclophosphamide in mouse and human oocytes by use of MDR inhibitors. Here we use genetic deletions in MDR1a/b/BCRP of mice to test MDR function in ovarian somatic cells and find that mdr1a/b/bcrp-/- mice had significantly increased sensitivity to cyclophosphamide...
February 12, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28216326/a-scalable-lysyl-hydroxylase-2-expression-system-and-luciferase-based-enzymatic-activity-assay
#7
Hou-Fu Guo, Eun Jeong Cho, Ashwini K Devkota, Yulong Chen, William Russell, George N Phillips, Mitsuo Yamauchi, Kevin N Dalby, Jonathan M Kurie
Hydroxylysine aldehyde-derived collagen cross-links (HLCCs) accumulate in fibrotic tissues and certain types of cancer and are thought to drive the progression of these diseases. HLCC formation is initiated by lysyl hydroxylase 2 (LH2), an Fe(II) and α-ketoglutarate (αKG)-dependent oxygenase that hydroxylates telopeptidyl lysine residues on collagen. Development of LH2 antagonists for the treatment of these diseases will require a reliable source of recombinant LH2 protein and a non-radioactive LH2 enzymatic activity assay that is amenable to high throughput screens of small molecule libraries...
February 16, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28214889/regulatory-role-of-mirna-375-in-expression-of-bmp15-gdf9-receptors-and-its-effect-on-proliferation-and-apoptosis-of-bovine-cumulus-cells
#8
Hongyan Chen, Chang Liu, Hao Jiang, Yan Gao, Mingqiang Xu, Jiawei Wang, Siyuan Liu, Yao Fu, Xulei Sun, Jiajun Xu, Jiabao Zhang, Lisheng Dai
BACKGROUND: Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are members of the transforming growth factor beta (TGF-β) superfamily. Through autocrine and paracrine mechanisms, these two factors can regulate cell differentiation, proliferation, and other functions in the ovary locally. Furthermore, GDF9 and BMP15 play vital roles in follicular growth, atresia, ovulation, fertilization, reproduction, and maintenance. Numerous studies have demonstrated a synergy between BMP15 and GDF9...
January 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28212834/death-associated-protein-1-dap-1-positively-regulates-virus-replication-and-apoptosis-of-hemocytes-in-shrimp-marsupenaeus-japonicus
#9
Wen-Li Xia, Li-Hua Kang, Chang-Bin Liu, Cui-Jie Kang
Death-associated protein 1 (DAP1) is a small proline-rich cytoplasmic protein that functions both in the apoptosis and autophage process of mammalian and in the clinical cancer of human. However, little knowledge is known about the homologue gene of DAP1 and its roles in the physiological process of invertebrates. In this paper, we report a novel function of DAP1 in the antivirus immunity of shrimp. A homologue gene of DAP1 was cloned from Marsupenaeus japonicus and named as Mjdap-1. The full-length of Mjdap-1 was 1761 bp with a 309 bp open reading frame that encoded 102 amino acids...
February 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28202331/genetics-of-gynaecological-cancers
#10
REVIEW
Panayiotis Constantinou, Marc Tischkowitz
Gynaecological cancers accounted for 16.3% of all cancers and 13.9% of all cancer deaths in women globally in 2012. Cancer of the cervix is the most common gynaecological cancer, followed by cancers of the uterus and the ovary. Although cervical cancer is almost exclusively triggered by human papilloma virus infection, approximately 5% of all uterine cancers and 20% of all ovarian cancers are caused by germline mutations in cancer predisposition genes. A number of genetic syndromes are associated with rarer gynaecological tumours...
January 24, 2017: Best Practice & Research. Clinical Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/28196955/identification-of-human-junctional-adhesion-molecule-1-as-a-functional-receptor-for-the-hom-1-calicivirus-on-human-cells
#11
Stanislav V Sosnovtsev, Carlos Sandoval-Jaime, Gabriel I Parra, Christine M Tin, Ronald W Jones, Jo Soden, Donna Barnes, Jim Freeth, Alvin W Smith, Kim Y Green
The Hom-1 vesivirus was reported in 1998 following the inadvertent transmission of the animal calicivirus San Miguel sea lion virus to a human host in a laboratory. We characterized the Hom-1 strain and investigated the mechanism by which human cells could be infected. An expression library of 3,559 human plasma membrane proteins was screened for reactivity with Hom-1 virus-like particles, and a single interacting protein, human junctional adhesion molecule 1 (hJAM1), was identified. Transient expression of hJAM1 conferred susceptibility to Hom-1 infection on nonpermissive Chinese hamster ovary (CHO) cells...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28185934/corticosteroids-deeply-depress-the-in-vitro-steroidogenic-capacity-of-eurasian-perch-ovary-at-the-end-of-the-reproductive-cycle
#12
S N M Mandiki, S Milla, S Nkogo Robles, P Kestemont
Corticosteroids play positive or negative role in the reproductive mechanisms of many fish species but the physiological contexts relating to such biphasic actions are not well defined. In the present study we investigated to what extent corticosteroids (cortisol-Co, 11-deoxycorticosterone-DOC) hormones may interfere with the steroidogenic capacity of Eurasian perch follicles, and we tested whether the negative interference of corticosteroids may be mitigated by potential stimulating endocrine factors, namely insulin-like growth factor-1 (IGF), human chorionic gonadotropin (HCG) or thyroid hormones (Triidothyronine-T3, thyroxine-T4)...
February 6, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28178894/adenocarcinoma-of-the-lung-metastatic-to-the-ovary-with-a-signet-ring-cell-component
#13
Giovanna Giordano, Nelly Cruz Viruel, Enrico Maria Silini, Francisco F Nogales
A nonsmoker 45-year-old woman, presented with a solid right ovarian mass. Microscopic examination revealed heterogeneous histology with tubular formations and extensive signet ring cell component that resembled the usual appearance of metastatic gastric carcinoma to the ovary. Moreover, the histology also showed solid nests of cells with a microvacuolated basophilic cytoplasm similar to those found in adenosquamous cervical carcinoma of glassy cell type. However, analysis of the patient's past history revealed a lung adenocarcinoma, diagnosed 4 years before, which prompted an immunohistochemical differential diagnosis, showing a strong expression for TTF-1 and Napsin A...
February 1, 2017: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28178702/dysregulation-of-the-expression-of-asparagine-linked-glycosylation-13-short-isoform-2-affects-nephrin-function-by-altering-its-n-linked-glycosylation
#14
Teresa Esposito, Giovanni De Stefano, Mafalda Giovanna Reccia, Ilaria Di Lorenzo, Filomena Napolitano, Francesco Scalabrì, Alessia Lombardi, Moin A Saleem, Lyn R Griffiths, Fernando Gianfrancesco
BACKGROUND: N-linked glycosylation, which is a post-translational modification process, plays an important role in protein folding, intracellular trafficking and membrane targeting, as well as in regulating the protein function. Recently, we identified a missense variant (p.T141L) in the short isoform 2 of the X-linked gene asparagine-linked glycosylation 13 (ALG13-is2), which segregated with focal segmental glomerulosclerosis and PCCD in a large Australian pedigree; however, any evidence of its pathogenicity was demonstrated...
February 9, 2017: Nephron
https://www.readbyqxmd.com/read/28178600/whole-flaxseed-diet-alters-estrogen-metabolism-to-promote-2-methoxtestradiol-induced-apoptosis-in-hen-ovarian-cancer
#15
Anushka Dikshit, Karen Hales, Dale Buchanan Hales
The study reported here demonstrates that a flaxseed-supplemented diet causes ovarian tumors in the laying hen to undergo apoptosis, resulting in a reduction of tumor burden, reducing the frequency and severity of ovarian cancer. We have previously shown in normal ovaries that flaxseed and its components down-regulate ERalpha and alter the expression of enzymes that metabolize estrogen. In this study, we analyzed the effects of the two main components of whole flaxseed, ligan and omega 3 fatty acids on estrogen metabolism and the estrogen receptor in ovarian tumors...
January 23, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28169296/small-molecule-biased-formyl-peptide-receptor-agonist-compound-17b-protects-against-myocardial-ischaemia-reperfusion-injury-in-mice
#16
Cheng Xue Qin, Lauren T May, Renming Li, Nga Cao, Sarah Rosli, Minh Deo, Amy E Alexander, Duncan Horlock, Jane E Bourke, Yuan H Yang, Alastair G Stewart, David M Kaye, Xiao-Jun Du, Patrick M Sexton, Arthur Christopoulos, Xiao-Ming Gao, Rebecca H Ritchie
Effective treatment for managing myocardial infarction (MI) remains an urgent, unmet clinical need. Formyl peptide receptors (FPR) regulate inflammation, a major contributing mechanism to cardiac injury following MI. Here we demonstrate that FPR1/FPR2-biased agonism may represent a novel therapeutic strategy for the treatment of MI. The small-molecule FPR1/FPR2 agonist, Compound 17b (Cmpd17b), exhibits a distinct signalling fingerprint to the conventional FPR1/FPR2 agonist, Compound-43 (Cmpd43). In Chinese hamster ovary (CHO) cells stably transfected with human FPR1 or FPR2, Compd17b is biased away from potentially detrimental FPR1/2-mediated calcium mobilization, but retains the pro-survival signalling, ERK1/2 and Akt phosphorylation, relative to Compd43...
February 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/28168653/effects-of-a-hyperandrogenaemic-state-on-the-proliferation-and-decidualization-potential-in-human-endometrial-stromal-cells
#17
Alexander Freis, Tobias Renke, Ulrike Kämmerer, Julia Jauckus, Thomas Strowitzki, Ariane Germeyer
OBJECTIVE: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women, involving hyperandrogenaemia and insulin resistance. Treatment options include dexamethasone, as well as the off-label use of metformin. To evaluate the impact of those drugs on cyclic changes in endometrial development, we tested possible effects of metformin and dexamethasone on endometrial stromal cells decidualisation, proliferation, and gene regulation in a hyperandrogenaemic microenvironment in vitro...
February 6, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28166994/deoxynivalenol-induces-toxic-effects-in-the-ovaries-of-pigs-an-ex%C3%A2-vivo-approach
#18
Juliana Rubira Gerez, Stela Silva Desto, Ana Paula Frederico Rodrigues Loureiro Bracarense
Deoxynivalenol (DON) is a frequently found trichothecene mycotoxin that elicits toxic effects on humans and animals. In pigs, DON induces changes in digestive and immune systems. Effects on the reproductive system are scarce and mainly based in in vitro models. The aim of this study was to evaluate, using an ex vivo model, the effects of DON on the morphology of ovaries of pigs in all stages of follicular development. Six 5-month-old pigs were used for sampling the explants. Thirty-six explants were incubated for 48 hours in culture medium (n = 18) or medium containing 10 μM of DON (n = 18)...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28165157/atf6%C3%AE-based-fine-tuning-of-the-unfolded-protein-response-enhances-therapeutic-antibody-productivity-of-chinese-hamster-ovary-cells
#19
Lisa A Pieper, Michaela Strotbek, Till Wenger, Monilola A Olayioye, Angelika Hausser
The dynamics of protein folding and secretion are key issues in improving the productivity and robustness of Chinese Hamster Ovary (CHO) producer cells. High recombinant protein secretion in CHO producer clones triggers the activation of the unfolded protein response (UPR), an intracellular response to the accumulation of unfolded and misfolded proteins in the endoplasmic reticulum (ER). We previously reported that the human microRNA (miRNA) miR-1287 enhances productivity in IgG expressing CHO cells (CHO-IgG)...
February 6, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28155604/a-vector-based-on-the-chicken-hypersensitive-site-4-insulator-element-replicates-episomally-in-mammalian-cell
#20
Xi Zhang, Xiao-Yin Wang, Yan-Long Jia, Xiao Guo, Yan-Fang Wang, Tian-Yun Wang
Gene therapy in mammalian cells requires vectors exhibiting long-term stability and high expression. Episomal gene expression vectors offer a safe and attractive alternative to those that integrate into the host cell genome. In the present study, we developed a new episomal vector based on the insulator, chicken hypersensitive site 4 (cHS4). The cHS4 element was artificially synthesized, cloned into the pEGFP-C1 vector, and used to transfect Chinese hamster ovary (CHO) and human Chang liver cells. The stably transfected cell colonies were further cultured in either the presence or absence of G418 selection...
February 2, 2017: Current Gene Therapy
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