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https://www.readbyqxmd.com/read/28202229/requirement-of-the-rna-binding-protein-smpb-during-intracellular-growth-of-listeria-monocytogenes
#1
Mobarak Abu Mraheil, Renate Frantz, Lisa Teubner, Heiko Wendt, Uwe Linne, Jessica Wingerath, Thomas Wirth, Trinad Chakraborty
Bacterial trans-translation is the main quality control mechanism employed to relieve stalled ribosomes. Trans-translation is mediated by the small protein B (SmpB) and transfer-mRNA (tmRNA) ribonucleoprotein complex, which interacts with translational complexes stalled at the 3' end of non-stop mRNAs to release the stalled ribosomes thereby targeting the nascent polypeptides and truncated mRNAs for degradation. The trans-translation system exists with a few exceptions in all bacteria. In the present study, we assessed the contribution of SmpB to the growth and virulence of Listeria monocytogenes, a human intracellular food-borne pathogen that colonizes host tissues to cause severe invasive infections...
February 1, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28138068/ribosome-dynamics-during-decoding
#2
REVIEW
Marina V Rodnina, Niels Fischer, Cristina Maracci, Holger Stark
Elongation factors Tu (EF-Tu) and SelB are translational GTPases that deliver aminoacyl-tRNAs (aa-tRNAs) to the ribosome. In each canonical round of translation elongation, aa-tRNAs, assisted by EF-Tu, decode mRNA codons and insert the respective amino acid into the growing peptide chain. Stop codons usually lead to translation termination; however, in special cases UGA codons are recoded to selenocysteine (Sec) with the help of SelB. Recruitment of EF-Tu and SelB together with their respective aa-tRNAs to the ribosome is a multistep process...
March 19, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28132884/novel-mutation-in-mitochondrial-elongation-factor-ef-tu-associated-to-dysplastic-leukoencephalopathy-and-defective-mitochondrial-dna-translation
#3
Michela Di Nottia, Arianna Montanari, Daniela Verrigni, Romina Oliva, Alessandra Torraco, Erika Fernandez-Vizarra, Daria Diodato, Teresa Rizza, Marzia Bianchi, Michela Catteruccia, Massimo Zeviani, Carlo Dionisi-Vici, Silvia Francisci, Enrico Bertini, Rosalba Carrozzo
The mitochondrial Elongation Factor Tu (EF-Tu), encoded by the TUFM gene, is a highly conserved GTPase, which is part of the mitochondrial protein translation machinery. In its activated form it delivers the aminoacyl-tRNAs to the A site of the mitochondrial ribosome. We report here on a baby girl with severe infantile macrocystic leukodystrophy with micropolygyria and a combined defect of complexes I and IV in muscle biopsy, caused by a novel mutation identified in TUFM. Using human mutant cells and the yeast model, we demonstrate the pathological role of the novel variant...
January 26, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28130490/duplication-of-drosophila-melanogaster-mitochondrial-ef-tu-pre-adaptation-to-t-arm-truncation-and-exclusion-of-bulky-aminoacyl-residues
#4
Aya Sato, Takuma Suematsu, Koh-Ki Aihara, Kiyoshi Kita, Tsutomu Suzuki, Kimitsuna Watanabe, Takashi Ohtsuki, Yoh-Ichi Watanabe
Translation elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA to ribosomes in protein synthesis. EF-Tu generally recognizes aminoacyl moieties and acceptor- and T-stems of aminoacyl-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively recognize mt tRNAs lacking T-arms and D-arms in the mitochondria of the chromadorean nematode Caenorhabditis elegans C...
January 27, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28104890/the-receptor-kinase-fer-is-a-ralf-regulated-scaffold-controlling-plant-immune-signaling
#5
Martin Stegmann, Jacqueline Monaghan, Elwira Smakowska-Luzan, Hanna Rovenich, Anita Lehner, Nicholas Holton, Youssef Belkhadir, Cyril Zipfel
In plants, perception of invading pathogens involves cell-surface immune receptor kinases. Here, we report that the Arabidopsis SITE-1 PROTEASE (S1P) cleaves endogenous RAPID ALKALINIZATION FACTOR (RALF) propeptides to inhibit plant immunity. This inhibition is mediated by the malectin-like receptor kinase FERONIA (FER), which otherwise facilitates the ligand-induced complex formation of the immune receptor kinases EF-TU RECEPTOR (EFR) and FLAGELLIN-SENSING 2 (FLS2) with their co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) to initiate immune signaling...
January 20, 2017: Science
https://www.readbyqxmd.com/read/28089773/mitochondrial-diseases-yeast-as-a-model-for-the-study-of-suppressors
#6
Silvia Francisci, Arianna Montanari
Mitochondrial (mt) tRNA gene mutations are an important cause of human morbidity and are associated with different syndromes. We have previously shown that the mitochondrial protein synthesis elongation factor EF-Tu and isolated sequences from the carboxy-terminal domain of yeast and human mt leucyl-tRNA synthetases (LeuRS), have a wide range of suppression capability among different yeast mt tRNA mutants having defective respiratory phenotype. Here we show that the rescuing capability can be restricted to a specific sequence of six amino acids from the carboxy-terminal domain of mt LeuRS...
January 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28043507/t-cell-cytokine-responses-in-peripheral-blood-mononuclear-cells-from-patients-with-multidrug-resistant-tuberculosis-following-stimulation-with-proteins-purified-from-mycobacterium-tuberculosis-mdr-clinical-isolates
#7
Alireza Hadizadeh Tasbiti, Shamsi Yari, Mostafa Ghanei, Seyed Davar Siadat, Amir Amanzadeh, Payam Tabarsi, Keyvan Saeedfar, Ahmadreza Bahrmand
OBJECTIVE: Tuberculosis (TB) is a devastating disease that remains a major health threat worldwide. The appearance of Mycobacterium tuberculosis strains resistance to current antibiotics is a growing problem, both in the third world and in developed countries. Completion of genomic sequencing of M. tuberculosis provides a strong foundation for subsequent identification of proteins to aid the understanding of protein function and the discovery of new drug targets or a TB vaccine. This study employed a proteomics approach to identify proteins from antibiotic resistant M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/27943049/functional-properties-of-lactobacillus-mucosae-strains-isolated-from-brazilian-goat-milk
#8
Georgia Maciel Dias de Moraes, Louricélia Rodrigues de Abreu, Antônio Silvio do Egito, Hévila Oliveira Salles, Liana Maria Ferreira da Silva, Luís Augusto Nero, Svetoslav Dimitrov Todorov, Karina Maria Olbrich Dos Santos
The search for probiotic candidates among lactic acid bacteria (LAB) isolated from food may uncover new strains with promising health and technological properties. Lactobacillus mucosae strains attracted recent research attention due to their ability to adhere to intestinal mucus and to inhibit pathogens in the gastrointestinal tract, both related to a probiotic potential. Properties of interest and safety aspects of three Lb. mucosae strains (CNPC006, CNPC007, and CNPC009) isolated from goat milk were investigated employing in vitro tests...
December 10, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27865402/some-proteins-of-m-%C3%A2-tuberculosis-that-localise-to-the-nucleus-of-thp-1-derived-macrophages
#9
Atul Kumar Agrawal, Rajeev Ranjan, Sharat Chandra, Tofan Kumar Rout, Amit Misra, Thota Jagadeshwar Reddy
Host-pathogen dialectics in tuberculosis (TB) via DNA-protein interactions are emerging. We investigated whether proteins produced by Mycobacterium tuberculosis (Mtb) could translocate to the host nucleus. Using lysates of nuclei purified from Mtb-infected THP-1-derived macrophages, we identified at least 15 proteins of Mtb-origin by electrophoretic and chromatographic separation and mass spectrometry. Western blotting confirmed time-dependent accumulation of Mtb EF-Tu, GroEL, GroES and MtrA in the host nucleus...
December 2016: Tuberculosis
https://www.readbyqxmd.com/read/27837019/two-proofreading-steps-amplify-the-accuracy-of-genetic-code-translation
#10
Ka-Weng Ieong, Ülkü Uzun, Maria Selmer, Måns Ehrenberg
Aminoacyl-tRNAs (aa-tRNAs) are selected by the messenger RNA programmed ribosome in ternary complex with elongation factor Tu (EF-Tu) and GTP and then, again, in a proofreading step after GTP hydrolysis on EF-Tu. We use tRNA mutants with different affinities for EF-Tu to demonstrate that proofreading of aa-tRNAs occurs in two consecutive steps. First, aa-tRNAs in ternary complex with EF-Tu·GDP are selected in a step where the accuracy increases linearly with increasing aa-tRNA affinity to EF-Tu. Then, following dissociation of EF-Tu·GDP from the ribosome, the accuracy is further increased in a second and apparently EF-Tu-independent step...
November 29, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27796304/how-ef-tu-can-contribute-to-efficient-proofreading-of-aa-trna-by-the-ribosome
#11
Jeffrey K Noel, Paul C Whitford
It has long been recognized that the thermodynamics of mRNA-tRNA base pairing is insufficient to explain the high fidelity and efficiency of aminoacyl-tRNA (aa-tRNA) selection by the ribosome. To rationalize this apparent inconsistency, Hopfield proposed that the ribosome may improve accuracy by utilizing a multi-step kinetic proofreading mechanism. While biochemical, structural and single-molecule studies have provided a detailed characterization of aa-tRNA selection, there is a limited understanding of how the physical-chemical properties of the ribosome enable proofreading...
October 31, 2016: Nature Communications
https://www.readbyqxmd.com/read/27764940/secretomic-analysis-of-host-pathogen-interactions-reveals-that-elongation-factor-tu-is-a-potential-adherence-factor-of-helicobacter-pylori-during-pathogenesis
#12
Kuo-Hsun Chiu, Ling-Hui Wang, Tsung-Ting Tsai, Huan-Yao Lei, Pao-Chi Liao
The secreted proteins of bacteria are usually accompanied by virulence factors, which can cause inflammation and damage host cells. Identifying the secretomes arising from the interactions of bacteria and host cells could, therefore, increase understanding of the mechanisms during initial pathogenesis. The present study used a host-pathogen coculture system of Helicobacter pylori and monocytes (THP-1 cells) to investigate the secreted proteins associated with initial H. pylori pathogenesis. The secreted proteins from the conditioned media from H...
October 20, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27708257/crystal-structures-of-the-human-elongation-factor-eefsec-suggest-a-non-canonical-mechanism-for-selenocysteine-incorporation
#13
Malgorzata Dobosz-Bartoszek, Mark H Pinkerton, Zbyszek Otwinowski, Srinivas Chakravarthy, Dieter Söll, Paul R Copeland, Miljan Simonović
Selenocysteine is the only proteinogenic amino acid encoded by a recoded in-frame UGA codon that does not operate as the canonical opal stop codon. A specialized translation elongation factor, eEFSec in eukaryotes and SelB in prokaryotes, promotes selenocysteine incorporation into selenoproteins by a still poorly understood mechanism. Our structural and biochemical results reveal that four domains of human eEFSec fold into a chalice-like structure that has similar binding affinities for GDP, GTP and other guanine nucleotides...
October 6, 2016: Nature Communications
https://www.readbyqxmd.com/read/27651493/clathrin-dependent-endocytosis-is-required-for-immunity-mediated-by-pattern-recognition-receptor-kinases
#14
Malick Mbengue, Gildas Bourdais, Fabio Gervasi, Martina Beck, Ji Zhou, Thomas Spallek, Sebastian Bartels, Thomas Boller, Takashi Ueda, Hannah Kuhn, Silke Robatzek
Sensing of potential pathogenic bacteria is of critical importance for immunity. In plants, this involves plasma membrane-resident pattern recognition receptors, one of which is the FLAGELLIN SENSING 2 (FLS2) receptor kinase. Ligand-activated FLS2 receptors are internalized into endosomes. However, the extent to which these spatiotemporal dynamics are generally present among pattern recognition receptors (PRRs) and their regulation remain elusive. Using live-cell imaging, we show that at least three other receptor kinases associated with plant immunity, PEP RECEPTOR 1/2 (PEPR1/2) and EF-TU RECEPTOR (EFR), internalize in a ligand-specific manner...
September 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27640531/actinomyces-spp-gene-expression-in-root-caries-lesions
#15
Naile Dame-Teixeira, Clarissa Cavalcanti Fatturi Parolo, Marisa Maltz, Aradhna Tugnait, Deirdre Devine, Thuy Do
BACKGROUND: The studies of the distribution of Actinomyces spp. on carious and non-carious root surfaces have not been able to confirm the association of these bacteria with root caries, although they were extensively implicated as a prime suspect in root caries. OBJECTIVE: The aim of this study was to observe the gene expression of Actinomyces spp. in the microbiota of root surfaces with and without caries. DESIGN: The oral biofilms from exposed sound root surface (SRS; n=10) and active root caries (RC; n=30) samples were collected...
2016: Journal of Oral Microbiology
https://www.readbyqxmd.com/read/27634774/identification-and-characterisation-of-elongation-factor-tu-a-novel-protein-involved-in-paracoccidioides-brasiliensis-host-interaction
#16
Caroline Maria Marcos, Haroldo Cesar de Oliveira, Julhiany de Fátima da Silva, Patricia Akemi Assato, Daniella Sayuri Yamazaki, Rosângela Aparecida Moraes da Silva, Cláudia Tavares Santos, Norival Alves Santos-Filho, Deivys Leandro Portuondo, Maria José Soares Mendes-Giannini, Ana Marisa Fusco-Almeida
Paracoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in Latin America, the paracoccidioidomycosis. The aim of this study was to characterise the involvement of elongation factor Tu (EF-Tu) in Paracoccidioides brasiliensis-host interaction. Adhesive properties were examined using recombinant PbEF-Tu proteins and the respective polyclonal anti-rPbEF-Tu antibody. Immunogold analysis demonstrated the surface location of EF-Tu in P...
November 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27624934/the-c-terminal-helix-of-pseudomonas-aeruginosa-elongation-factor-ts-tunes-ef-tu-dynamics-to-modulate-nucleotide-exchange
#17
Evelina Ines De Laurentiis, Evan Mercier, Hans-Joachim Wieden
Little is known about the conservation of critical kinetic parameters and the mechanistic strategies of elongation factor (EF) Ts-catalyzed nucleotide exchange in EF-Tu in bacteria and particularly in clinically relevant pathogens. EF-Tu from the clinically relevant pathogen Pseudomonas aeruginosa shares over 84% sequence identity with the corresponding elongation factor from Escherichia coli Interestingly, the functionally closely linked EF-Ts only shares 55% sequence identity. To identify any differences in the nucleotide binding properties, as well as in the EF-Ts-mediated nucleotide exchange reaction, we performed a comparative rapid kinetics and mutagenesis analysis of the nucleotide exchange mechanism for both the E...
October 28, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27573234/morphogenetic-and-molecular-analyses-of-cyst-wall-components-in-the-ciliated-protozoan-colpoda-cucullus-nag-1
#18
Ryoji Funadani, Yoichiro Sogame, Katsuhiko Kojima, Toshikazu Takeshita, Keisuke Yamamoto, Tomoya Tsujizono, Futoshi Suizu, Seiji Miyata, Ken-Ichi Yagyu, Tomohiko Suzuki, Mikihiko Arikawa, Tatsuomi Matsuoka
The cyst wall of the resting cyst of the ciliated protozoan Colpoda cucullus (Nag-1 strain) is composed of several layers of endocyst, a single layer of ectocyst associated with a mucous layer and lepidosomes composed of a fibrous or crystal-like structure. SDS-PAGE showed that the ectocyst associated with lepidosomes and mucous materials contained proteins corresponding to 27 kDa, 31 kDa, 45 kDa and smear bands ranging from 50 to 60 kDa. LC-MS/MS of these proteins revealed that the 45-kDa protein (p45) was elongation factor Tu (EF-Tu)...
August 28, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27553416/micrococcin-p1-a-bactericidal-thiopeptide-active-against-mycobacterium-tuberculosis
#19
Giulia Degiacomi, Yoann Personne, Guillaume Mondésert, Xueliang Ge, Chandra Sekhar Mandava, Ruben C Hartkoorn, Francesca Boldrin, Pavitra Goel, Kristin Peisker, Andrej Benjak, Maria Belén Barrio, Marcello Ventura, Amanda C Brown, Véronique Leblanc, Armin Bauer, Suparna Sanyal, Stewart T Cole, Sophie Lagrange, Tanya Parish, Riccardo Manganelli
The lack of proper treatment for serious infectious diseases due to the emergence of multidrug resistance reinforces the need for the discovery of novel antibiotics. This is particularly true for tuberculosis (TB) for which 3.7% of new cases and 20% of previously treated cases are estimated to be caused by multi-drug resistant strains. In addition, in the case of TB, which claimed 1.5 million lives in 2014, the treatment of the least complicated, drug sensitive cases is lengthy and disagreeable. Therefore, new drugs with novel targets are urgently needed to control resistant Mycobacterium tuberculosis strains...
September 2016: Tuberculosis
https://www.readbyqxmd.com/read/27537186/elongation-factor-tu-and-heat-shock-protein-70-are-membrane-associated-proteins-from-mycoplasma-ovipneumoniae-capable-of-inducing-strong-immune-response-in-mice
#20
Fei Jiang, Jinyan He, Nalu Navarro-Alvarez, Jian Xu, Xia Li, Peng Li, Wenxue Wu
Chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry. Mycoplasma ovipneumoniae (M. ovipneumoniae) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold and immunoblotting that elongation factor Tu (EF-Tu) and heat shock protein 70 (HSP 70) are membrane-associated proteins on M. ovipneumonaiea. We have evaluated the humoral and cellular immune responses in vivo by immunizing BALB/c mice with both purified recombinant proteins rEF-Tu and rHSP70...
2016: PloS One
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