Garrett Deletti, Sajan D Green, Caleb Weber, Kristen N Patterson, Swapnil S Joshi, Tushar M Khopade, Mathew Coban, James Veek-Wilson, Thomas R Caulfield, Rajesh Viswanathan, Amy L Lane
The 2,5-diketopiperazines are a prominent class of bioactive molecules. The nocardioazines are actinomycete natural products that feature a pyrroloindoline diketopiperazine scaffold composed of two D-tryptophan residues functionalized by N- and C-methylation, prenylation, and diannulation. Here we identify and characterize the nocardioazine B biosynthetic pathway from marine Nocardiopsis sp. CMB-M0232 by using heterologous biotransformations, in vitro biochemical assays, and macromolecular modeling. Assembly of the cyclo-L-Trp-L-Trp diketopiperazine precursor is catalyzed by a cyclodipeptide synthase...
May 3, 2023: Nature Communications