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Wen-Yi Zhang, Cui-Juan Niu, Bo-Jian Chen, Kenneth B Storey
Turtles are well known for their stress tolerance, including an ability to deal with temperature extremes or rapid thermal change. To know more about the comprehensive molecular basis of thermal stress responses in turtles, we assessed differentially expressed genes (DEGs) in the brain, liver and kidney of juvenile soft-shelled turtles, Pelodiscus sinensis, after acute cold stress (28 °C-8 °C acute transfer and held for 12 h) and following recovery (back to 28 °C and held for 24 h) by digital gene expression profiling...
February 20, 2018: Cryobiology
Meijun Yuan, Mingxiao Ning, Panpan Wei, Wenjing Hao, Yunting Jing, Wei Gu, Wen Wang, Qingguo Meng
Serpin families classified serine protease inhibitors regulate various physiological processes. However, there is not study on the role of serpin in immune responses against Spiroplasma eriocheiris as a novel causative pathogen in the Chinese mitten crab, Eriocheir sinensis. In our study, quantitative real-time PCR (qRT-PCR) revealed that the mRNA transcripts of Esserpin-2 were ubiquitous in every tissue, relative higher expression in hepatopancreas, gill and hemocytes, while the intestine, muscle, heart and nerve showed relative lower expression...
February 20, 2018: Fish & Shellfish Immunology
A Castellano-Hinojosa, P Maza-Márquez, Y Melero-Rubio, J González-López, B Rodelas
Ammonia-oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA) and N2 O-reducing denitrifiers were measured by quantitative real-time PCR (qPCR) in activated sludge samples from four full-scale wastewater treatment plants (WWTPs) in South Spain, and their abundances were linked to the generation of N2 O in the samples using multivariate analysis (Non-metric multidimensional scaling, MDS, and BIO-ENV). The average abundances of AOA remained in similar orders of magnitude in all WWTPs (106 copies amoA/L activated sludge mixed liquor), while significant differences were detected for AOB (105 -109 copies amoA/L) and N2 O-reducers (107 -1010 copies nosZ/L)...
February 19, 2018: Chemosphere
Anu Aggarwal, Manu Jamwal, Ganesh Kumar V, Prashant Sharma, Man Updesh Singh Sachdeva, Deepak Bansal, Pankaj Malhotra, Reena Das
Reference genes are indispensable for normalizing mRNA levels across samples in real-time quantitative PCR. Their expression levels vary under different experimental conditions and due to several inherent characteristics. Appropriate reference gene selection is thus critical for gene-expression studies. This study aimed at selecting optimal reference genes for gene-expression analysis of reticulocytes and validating them in hereditary spherocytosis (HS) and β-thalassemia intermedia (βTI) patients. Seven reference genes (PGK1, MPP1, HPRT1, ACTB, GAPDH, RN18S1, and SDHA) were selected from published literature...
February 20, 2018: Journal of Molecular Diagnostics: JMD
Susanne Kricke, Lana Mhaldien, Rozendo Fernandes, Charizel Villanueva, Alistair Shaw, Paul Veys, Stuart Adams
Certain blood components and anticoagulants interfere with the PCR process and subsequent analysis. Here we demonstrate that reliable test results can be obtained for chimerism analysis despite omitting a DNA extraction step and performing PCR and fragment analysis directly on bone marrow, whole-blood, and individual cell fractions. For chimerism analysis, this is possible with the use of a robust commercially available PCR mix containing a DNA polymerase capable of DNA amplification directly from the sample without the need for pre-treatment...
February 20, 2018: Journal of Molecular Diagnostics: JMD
Daniel P Feldmann, Yilong Cheng, Rima Kandil, Yuran Xie, Mariam Mohammadi, Hartmann Harz, Akhil Sharma, David J Peeler, Anna Moszczynska, Heinrich Leonhardt, Suzie H Pun, Olivia M Merkel
The block copolymer VIPER (virus-inspired polymer for endosomal release) has been reported to be a promising novel delivery system of DNA plasmids both in vitro and in vivo. VIPER is comprised of a polycation segment for condensation of nucleic acids as well as a pH-sensitive segment that exposes the membrane lytic peptide melittin in acidic environments to facilitate endosomal escape. The objective of this study was to investigate VIPER/siRNA polyplex characteristics, and compare their in vitro and in vivo performance with commercially available transfection reagents and a control version of VIPER lacking melittin...
February 20, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Qingling Yuan, Yang Liu, Yuxia Fan, Zheng Liu, Xiaoming Wang, Meng Jia, Zushi Geng, Jing Zhang, Xiubo Lu
BACKGROUND: Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy. Besides, increasing evidence has demonstrated that long non-coding RNA (lncRNA) HOTTIP played a crucial role in cancer pathogenesis. MiR-637-mediated Akt1 was involved in cell growth, invasion and migration in various malignancies. This study was aimed to investigate the potential biological effect and regulatory mechanism of HOTTIP on cell proliferation, invasion and migration in PTC. METHODS: Expression of HOTTIP, miR-637 and Akt1 were determined by quantitative RT-PCR (qRT-PCR) and western blotting in PTC tissues, normal tissues, PTC cells (TPC-1 and HTH83) or non-tumor thyroid cells (Nthy-ori 3-1)...
February 20, 2018: International Journal of Biochemistry & Cell Biology
Nadia Heydari, Novin Nikbakhsh, Farzin Sadeghi, Nazila Farnoush, Soraya Khafri, Milad Bastami, Hadi Parsian
AIMS: The purpose of the present study was to evaluate microRNA-140-3p expression level in breast cancer patients in comparison to healthy controls. PATIENTS & METHODS: Serum microRNA-140-3p level was quantified by realtime quantitative reverse transcription PCR in 40 women with breast cancer and 40 healthy subjects. RESULTS: Serum microRNA-140-3p level in patients compared to healthy subjects was significantly up-regulated (P = 0...
February 20, 2018: Gene
Man Liu, Guangbin Shi, Anyu Zhou, Cassady E Rupert, Kareen L K Coulombe, Samuel C Dudley
RATIONALE: Heart failure is characterized by electrical remodeling that contributes to arrhythmic risk. The unfolded protein response (UPR) is active in heart failure and can decrease protein levels by increasing mRNA decay, accelerating protein degradation, and inhibiting protein translation. OBJECTIVE: Therefore, we investigated whether the UPR downregulated cardiac ion channels that may contribute to arrhythmogenic electrical remodeling. METHODS: Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were used to study cardiac ion channels...
February 20, 2018: Journal of Molecular and Cellular Cardiology
Yixin Ge, Jae Hoon Lee, Baishi Hu, Youfu Frank Zhao
Extensive use of antibiotic streptomycin to control fire blight disease of apples and pears, caused by the enterobacterial plant pathogen Erwinia amylovora, leads to the development of streptomycin-resistant strains in the United States and elsewhere. Kasugamycin (Ksg, kasuminR) has been permitted to be used as an alternative or replacement to control this serious bacterial disease. In this study, we investigated the role of two major peptide ABC-transporter systems in E. amylovora, the dipeptide permease (Dpp) and oligopeptide permease (Opp), in conferring sensitivity to Ksg and blasticidin S (BcS)...
February 23, 2018: Molecular Plant-microbe Interactions: MPMI
Han Cheng, Xiang Chen, Jialin Fang, Zewei An, Yanshi Hu, Huasun Huang
The rubber tree (Hevea brasiliensis Muell. Arg) is a tropical, perennial, woody plant that is susceptible to cold stress. In China, cold stress has been found to severely damage rubber plants in plantations in past decades. Although several Hevea clones that are resistant to cold have been developed, their cold hardiness mechanism has yet to be elucidated. For the study reported herein, we subjected the cold-resistant clone CATAS93-114 and the cold-sensitive clone Reken501 to chilling stress, and characterized their transcriptomes at 0, 2, 8 and 24 h after the start of chilling...
February 21, 2018: Tree Physiology
Iris L A Bodewes, Shereen Al-Ali, Cornelia G van Helden-Meeuwsen, Naomi I Maria, Jessica Tarn, Dennis W Lendrem, Marco W J Schreurs, Eline C Steenwijk, Paul L A van Daele, Tim Both, Simon J Bowman, Bridget Griffiths, Wan-Fai Ng, Marjan A Versnel
Objective: To assess the relationships between systemic IFN type I (IFN-I) and II (IFN-II) activity and disease manifestations in primary SS (pSS). Methods: RT-PCR of multiple IFN-induced genes followed by principal component analysis of whole blood RNA of 50 pSS patients was used to identify indicator genes of systemic IFN-I and IFN-II activities. Systemic IFN activation levels were analysed in two independent European cohorts (n = 86 and 55, respectively) and their relationships with clinical features were analysed...
February 20, 2018: Rheumatology
Rosemary Gutierrez-Coarite, Nicole M Yoneishi, Ishakh Pulakkatu-Thodi, Javier Mollinedo, Bernarda Calla, Mark G Wright, Scott M Geib
Macadamia felted coccid, Eriococcus ironsidei (Williams) (Hemiptera: Eriococcidae) was first found infesting macadamia trees in the island of Hawaii in 2005. Macadamia felted coccid infests all above-ground parts of trees to feed and reproduce. Their feeding activity distorts and stunts new growth which causes yellow spotting on older leaves, and when population densities become high, branch dieback occurs. Different predatory beetles have been observed in macadamia nut trees infested by E. ironsidei, the most abundant were Halmus chalybeus, Curinus coeruleus, Scymnodes lividigaster, Rhyzobius forestieri, and Sticholotis ruficeps...
February 21, 2018: Journal of Economic Entomology
Ales Tichy, Sylwia Kabacik, Grainne O'Brien, Jaroslav Pejchal, Zuzana Sinkorova, Adela Kmochova, Igor Sirak, Andrea Malkova, Caterina Gomila Beltran, Juan Ramon Gonzalez, Jakub Grepl, Matthaeus Majewski, Elizabeth Ainsbury, Lenka Zarybnicka, Jana Vachelova, Alzbeta Zavrelova, Marie Davidkova, Marketa Markova Stastna, Michael Abend, Eileen Pernot, Elisabeth Cardis, Christophe Badie
The increasing risk of acute large-scale radiological/nuclear exposures of population underlines the necessity of developing new, rapid and high throughput biodosimetric tools for estimation of received dose and initial triage. We aimed to compare the induction and persistence of different radiation exposure biomarkers in human peripheral blood in vivo. Blood samples of patients with indicated radiotherapy (RT) undergoing partial body irradiation (PBI) were obtained soon before the first treatment and then after 24 h, 48 h, and 5 weeks; i...
2018: PloS One
Christel Gill Haanshuus, Sara Chandy, Anand Manoharan, Rosario Vivek, Dilip Mathai, Deepika Xena, Ashita Singh, Nina Langeland, Bjørn Blomberg, George Vasanthan, Usha Sitaram, Jonathan Appasamy, Joel Nesaraj, Anil Henry, Suvarna Patil, Gerardo Alvarez-Uria, Lois Armstrong, Kristine Mørch
[This corrects the article DOI: 10.1371/journal.pone.0158816.].
2018: PloS One
Yu-Hua Huang, Chiung-Mei Chen, Yun-Shien Lee, Kuo-Hsuan Chang, Huei-Wen Chen, Yi-Chun Chen
BACKGROUND: Coronary artery disease is associated with a common mitochondrial DNA alteration, a 4977 bp deletion (mtDNA4977). The role of mtDNA4977 in ischemic stroke is unknown. METHODS: Real-time quantitative PCR was performed to quantify total mtDNA and mtDNA4977 in leukocytes in 283 ischemic stroke cases and 135 controls. Ratios of mtDNA4977 to total-mtDNA and total-mtDNA to nuclear-DNA were calculated. Nested PCR and Sanger sequencing were used to confirm undetectable levels of mtDNA4977...
2018: PloS One
Denise Rabold, Werner Espelage, Muna Abu Sin, Tim Eckmanns, Alexander Schneeberg, Heinrich Neubauer, Nadine Möbius, Katja Hille, Lothar H Wieler, Christian Seyboldt, Antina Lübke-Becker
BACKGROUND: Clostridium difficile infections (CDI) in humans range from asymptomatic carriage to life-threatening intestinal disease. Findings on C. difficile in various animal species and an overlap in ribotypes (RTs) suggest potential zoonotic transmission. However, the impact of animals for human CDI remains unclear. METHODS: In a large-scale survey we collected 1,447 fecal samples to determine the occurrence of C. difficile in small companion animals (dogs and cats) and their owners and to assess potential epidemiological links within the community...
2018: PloS One
Shaoxi Niu, Xin Ma, Yu Zhang, Yen-Nien Liu, Xufeng Chen, Huijie Gong, Yuanxin Yao, Kan Liu, Xu Zhang
Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cell carcinoma, which shows high aggressiveness and lacks biomarkers. RhoB acts as a tumor suppressor that inhibits the progression of ccRCC. In the present study, we examined the effects of oncogenic microRNAs, miR-19a and miR-19b, on RhoB expression in ccRCC cells. The results showed that both miR-19a and miR-19b could directly target the 3'untranslated region (3'UTR) of RhoB, resulting in the reduced expression of RhoB. With RT-PCR analysis, we detected the increased expression of miR-19a and miR-19b in ccRCC tissues compared to adjacent non-tumor renal tissues...
2018: PloS One
Hua Yang, Yan Cai, Zhihang Zhuo, Wei Yang, Chunping Yang, Jin Zhang, Yang Yang, Baoxin Wang, Fengrong Guan
The white-striped longhorn beetle Batocera horsfieldi (Coleoptera: Cerambycidae) is a polyphagous wood-boring pest that causes substantial damage to the lumber industry. Moreover olfactory proteins are crucial components to function in related processes, but the B. horsfieldi genome is not readily available for olfactory proteins analysis. In the present study, developmental transcriptomes of larvae from the first instar to the prepupal stage, pupae, and adults (females and males) from emergence to mating were built by RNA sequencing to establish a genetic background that may help understand olfactory genes...
2018: PloS One
Rohani Ahmad, Ismail Suzilah, Wan Mohamad Ali Wan Najdah, Omar Topek, Ibrahim Mustafakamal, Han Lim Lee
A large scale study was conducted to elucidate the true relationship among entomological, epidemiological and environmental factors that contributed to dengue outbreak in Malaysia. Two large areas (Selayang and Bandar Baru Bangi) were selected in this study based on five consecutive years of high dengue cases. Entomological data were collected using ovitraps where the number of larvae was used to reflect Aedes mosquito population size; followed by RT-PCR screening to detect and serotype dengue virus in mosquitoes...
2018: PloS One
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