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https://www.readbyqxmd.com/read/29041002/astragaloside-iv-induced-mir-134-expression-reduces-emt-and-increases-chemotherapeutic-sensitivity-by-suppressing-creb1-signaling-in-colorectal-cancer-cell-line-sw-480
#1
Qi Ye, Li Su, Dagui Chen, Wenyi Zheng, Ye Liu
BACKGROUND: Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths worldwide. Although chemotherapy is the primary means in colorectal cancer treatment, it is burdenerd by adverse drug effects. Drug-resistance is one of the most important challenges for chemotherapy and epithelial-mesenchymal transition (EMT) plays critical role in the development of drug resistance. AIMS: The aim of this study was to investigate the mechanisms underlying the effect of astragaloside IV (AS-IV) on miR-134 expression, EMT and chemotherapeutic sensitivity in CRC...
October 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29040978/hydrogen-rich-saline-attenuates-brain-injury-induced-by-cardiopulmonary-bypass-and-inhibits-microvascular-endothelial-cell-apoptosis-via-the-pi3k-akt-gsk3%C3%AE-signaling-pathway-in-rats
#2
Keyan Chen, Nan Wang, Yugang Diao, Wanwei Dong, YingJie Sun, Lidan Liu, Xiuying Wu
BACKGROUND/AIMS: Cardiopulmonary bypass (CPB) is prone to inducing brain injury during open heart surgery. A hydrogen-rich solution (HRS) can prevent oxidation and apoptosis, and inhibit inflammation. This study investigated effects of HRS on brain injury induced by CPB and regulatory mechanisms of the PI3K/Akt/GSK3β signaling pathway. METHODS: A rat CPB model and an in vitro cell hypoxia model were established. After HRS treatment, Rat behavior was measured using neurological deficit score; Evans blue (EB) was used to assess permeability of the blood-brain barrier (BBB); HE staining was used to observe pathological changes; Inflammatory factors and brain injury markers were detected by ELISA; the PI3K/Akt/GSK3β pathway-related proteins and apoptosis were assessed by western blot, immunohistochemistry and qRT -PCR analyses of brain tissue and neurons...
October 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29040977/gene-microarray-integrated-with-high-throughput-proteomics-for-the-discovery-of-transthyretin-in-rhabdomyolysis-induced-acute-kidney-injury
#3
Ou Li, Xiaodong Geng, Qian Ma, Weiwei Wang, Ran Liu, Zhong Yin, Siyang Wang, Guangyan Cai, Xiangmei Chen, Quan Hong
BACKGROUND/AIMS: Rhabdomyolysis, one of the leading causes of acute kidney injury (AKI), develops after trauma, drug toxicity, infections, burns, and physical exertion. The aim of this study was to investigate differences in gene and protein expression to elucidate the pathogenesis of rhabdomyolysis (RM)-induced AKI. METHODS: In this study, we used glycerol induced renal injury as a model of RM-induced AKI. Affymetrix U133 plus 2.0 microarrays were used to perform gene microarray analysis...
October 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29040972/regulation-of-coronary-arterial-large-conductance-ca2-activated-k-channel-protein-expression-and-function-by-n-3-polyunsaturated-fatty-acids-in-diabetic-rats
#4
Xu Tang, Ling-Ling Qian, Ru-Xing Wang, Yong Yao, Shi-Peng Dang, Ying Wu, Wen Wang, Yuan Ji, Man-Qing Sun, Da-Yun Xia, Xiao-Yu Liu, Dai-Min Zhang, Qiang Chai, Tong Lu
AIM: The objective of this study was to examine the effects of n-3 polyunsaturated fatty acids (n-3 PUFAs) on coronary arterial large conductance Ca2+-activated K+ (BK) channel function in coronary smooth muscle cells (SMCs) of streptozotocin-induced diabetic rats. METHODS: The effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on coronary BK channel open probabilities were determined using the patch clamp technique. The mRNA and protein expressions of BK channel subunits were measured using qRT-PCR and Western blots...
October 18, 2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/29040964/polymorphisms-in-adrenergic-receptor-genes-in-qinchuan-cattle-show-associations-with-selected-carcass-traits
#5
Chu-Gang Mei, Lin-Sheng Gui, Hong-Cheng Wang, Wan-Qiang Tian, Yao-Kun Li, Lin-Sen Zan
The beta-adrenergic receptors coded by the ADRB1, ADRB2 and ADRB3 genes play important roles in mediating metabolic effects, especially lipolysis, insulin resistance and energy balance. This study investigated the expression levels of these three genes in different tissues of Qinchuan cattle by real-time polymerase chain reaction (RT-PCR). Expressed levels of RNA from the ADRB2 gene were generally much higher than for ADRB1 and ADRB3. ADRB1 and ADRB2 expression levels were highest in subcutaneous fat and lower in muscle, whereas ADRB3 expression was higher in muscle tissue...
October 12, 2017: Meat Science
https://www.readbyqxmd.com/read/29040585/emergence-of-mcr-1-and-carbapenemase-genes-in-hospital-sewage-water-in-beijing-china
#6
Longyang Jin, Ruobing Wang, Xiaojuan Wang, Qi Wang, Yawei Zhang, Yuyao Yin, Hui Wang
Objectives: This study identified and characterized mcr-1-positive Enterobacteriaceae (MCRPE) and carbapenemase-producing Enterobacteriaceae (CPE) in hospital sewage water. Methods: Influent and effluent sewage samples were collected from five tertiary hospitals in Beijing in December 2016. Samples were screened for MCRPE and CPE using antibiotic selection media. Results were confirmed by PCR amplification of β-lactamase and colistin resistance (mcr-1 and mcr-2) genes and by sequencing...
October 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29040583/taste-receptor-polymorphisms-and-male-infertility
#7
M Gentiluomo, L Crifasi, A Luddi, D Locci, R Barale, P Piomboni, D Campa
STUDY QUESTION: Are polymorphisms of taste receptor genes associated with male infertility? SUMMARY ANSWER: This study has showed the associations between three single nucleotide polymorphisms (SNPs) in taste receptors genes (TASR) and male infertility. WHAT IS KNOWN ALREADY: Recent studies showed the expression of taste receptors in the testis and in spermatozoa, suggesting their possible role in infertility. The vast genetic variability in taste genes results in a large degree of diversity in various human phenotypes...
October 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040578/systemic-inflammation-induced-by-micrornas-endometriosis-derived-alterations-in-circulating-microrna-125b-5p-and-let7b-5p-regulate-macrophage-cytokine-production
#8
Sepide E Nematian, Ramanaiah Mamillapalli, Trisha S Kadakia, Masoumeh Majidi Zolbin, Sarah Moustafa, Hugh S Taylor
Context: Endometriosis is characterized by aberrant inflammation. We previously reported increased levels of miRNA 125b-5p and decreased levels of miRNA let 7b-5p in serum of patients with endometriosis. Objective: Determine the regulatory function of microRNAs 125b-5p and Let 7b-5p on production of pro-inflammatory cytokines in endometriosis. Design: Case-control study. Setting: University hospital. Patients: Women with (20) and without (26) endometriosis; human U937 macrophage cell line...
October 12, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29040564/n-cadherin-identifies-human-endometrial-epithelial-progenitor-cells-by-in-vitro-stem-cell-assays
#9
Hong P T Nguyen, L Xiao, James A Deane, Ker-Sin Tan, Fiona L Cousins, Hirotaka Masuda, Carl N Sprung, Anna Rosamilia, Caroline E Gargett
STUDY QUESTION: Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity using in vitro assays? SUMMARY ANSWER: N-cadherin isolates clonogenic, self-renewing human endometrial epithelial progenitor cells with high proliferative potential that differentiate into cytokeratin+ gland-like structures in vitro and identifies their location in some cells of gland profiles predominantly in basalis endometrium adjacent to the myometrium...
September 27, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040526/phenology-of-the-potato-psyllid-bactericera-cockerelli-hemiptera-triozidae-and-candidatus-liberibacter-solanacearum-in-commercial-potato-fields-in-idaho
#10
Erik J Wenninger, Amy Carroll, Jennifer Dahan, Alexander V Karasev, Michael Thornton, Jeff Miller, Philip Nolte, Nora Olsen, William Price
Zebra chip disease (ZC) is an emerging disease of potato in which tubers are produced with striped necrotic patterns that make them unmarketable. ZC is associated with the bacterium "Candidatus Liberibacter solanacearum" (Lso), which is transmitted by the potato psyllid, Bactericera cockerelli (Šulc; Hemiptera: Triozidae). First found in Idaho during 2011, ZC now contributes to increased production costs each season via additional insecticide sprays. To clarify the extent and severity of the threat of ZC in Idaho, we sampled potato psyllids in commercial potato fields across the state over four growing seasons (2012-2015)...
October 12, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/29040520/clinical-implications-of-mitochondrial-dna-quantification-on-pregnancy-outcomes-a-blinded-prospective-non-selection-study
#11
Elpida Fragouli, Caroline McCaffrey, Krithika Ravichandran, Katharina Spath, James A Grifo, Santiago Munné, Dagan Wells
STUDY QUESTION: Can quantification of mitochondrial DNA (mtDNA) in trophectoderm (TE) biopsy samples provide information concerning the viability of a blastocyst, potentially enhancing embryo selection and improving IVF treatment outcomes? SUMMARY ANSWER: This study demonstrated that euploid blastocysts of good morphology, but with high mtDNA levels had a greatly reduced implantation potential. WHAT IS KNOWN ALREADY: Better methods of embryo selection leading to IVF outcome improvement are necessary, as the transfer of chromosomally normal embryos of high morphological grade cannot guarantee the establishment of an ongoing pregnancy...
September 23, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040474/co-occurrence-of-genes-for-aerobic-and-anaerobic-biodegradation-of-dichloroethane-in-organochlorine-contaminated-groundwater
#12
Jacob E Munro, Önder Kimyon, Deborah J Rich, Joanna Koenig, Sihui Tang, Adrian Low, Matthew Lee, Mike Manefield, Nicholas V Coleman
1,2-dichloroethane (DCA) is a problematic groundwater pollutant. Factors influencing the distribution and activities of DCA-degrading bacteria are not well-understood, which has hampered their application for bioremediation. Here, we used quantitative PCR (qPCR) to investigate the distribution of putative DCA-dehalogenating bacteria at a DCA-impacted site in Sydney (Australia). The dehalogenase genes dhlA, tceA and bvcA were detected in all groundwater samples (n = 15), while vcrA was found in 11/15 samples...
October 12, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29040467/validation-of-an-in-vitro-system-for-studies-of-pathogenicity-mechanisms-in-xanthomonas-campestris
#13
Mariana Rocha Maximiano, Osmundo B Oliveira-Neto, Octávio L Franco, Angela Mehta
Several minimal media capable of inducing pathogenicity genes have been used to study plant-pathogen interactions. An in planta assay to study a closer interaction between the bacteria and the host was also developed and has been employed by our group. In order to determine whether growth medium could be improved to better approximate in planta conditions beyond that offered by the defined minimal medium XVM1, we compared the expression of twenty Xanthomonas campestris pv. campestris (Xcc) genes by qRT-PCR under in vivo (bacteria recovered from the plant) and in vitro (rich medium NYG, minimal medium XVM1 and XVM1 + leaf extract) growth systems...
October 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29040443/a-dysbiosis-index-to-assess-microbial-changes-in-fecal-samples-of-dogs-with-chronic-inflammatory-enteropathy
#14
M K AlShawaqfeh, B Wajid, Y Minamoto, M Markel, J A Lidbury, J M Steiner, E Serpedin, J S Suchodolski
Recent studies have identified various bacterial groups that are altered in dogs with chronic inflammatory enteropathies (CE) compared to healthy dogs. The study aim was to use quantitative PCR (qPCR) assays to confirm these findings in a larger number of dogs, and to build a mathematical algorithm to report these microbiota changes as a dysbiosis index (DI). Fecal DNA from 95 healthy dogs and 106 dogs with histologically confirmed CE was analyzed. Samples were grouped into a training set and a validation set...
October 11, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29040223/effect-of-losartan-on-the-acute-response-of-human-elderly-skeletal-muscle-to-exercise
#15
Mette Flindt Heisterberg, Jesper L Andersen, Peter Schjerling, Jacob Bülow, Jeppe Bo Lauersen, Heidi L Roeber, Michael Kjaer, Abigail L Mackey
PURPOSE: To investigate the effect of blocking the angiotensin II type I receptor (AT1R) upon the response to acute heavy resistance exercise in elderly human skeletal muscle. The hypothesis was that AT1R blocking would result in a superior myogenic response accompanied by downregulation of TGF-β and upregulation of IGF-1 signalling. METHODS: 28 healthy elderly men (+64 years) were randomized into two groups, consuming either AT1R blocker (Losartan, 100mg/day) or Placebo for 18 days prior to exercise...
October 16, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29040159/new-approach-for-differentiation-of-bone-marrow-mesenchymal-stem-cells-toward-chondrocyte-cells-with-overexpression-of-microrna-140
#16
Mahboudi Hossein, Soleimani Masoud, Hanaee-Ahvaz Hana, Ghanbarian Hossein, Bandehpour Mojgan, Enderami Seyed Ehsan, Kazemi Bahram
Mesenchymal stem cells are widely stimulated by transforming growth factor beta-3 (TGFβ3) for chondrocyte differentiation. The objective of our study was to establish a new method for differentiation of human mesenchymal stem cells toward chondrocyte by overexpression of MicroRNA-140 (miR-140), and also this method was compared with method of induction with TGFβ3 in high-cell density culture systems. Mesenchymal stem cells were harvested from bone marrow of human. We prepared vectors and then was used for recombinant Lenti virus production in HEK-293 cell...
October 16, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29040083/consumption-of-edible-ice-contaminated-with-acinetobacter-pseudomonas-and-stenotrophomonas-is-a-risk-factor-for-fecal-colonization-with-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-vietnam
#17
Tatsuya Nakayama, Nguyen Cong Ha, Phong Quoc Le, Ryuji Kawahara, Yuko Kumeda, Yoshinori Sumimura, Yoshimasa Yamamoto
Although Vietnamese residents frequently harbor extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E), it is unclear which foods/beverages are risk factors for acquiring these bacteria. The aim of this study was to evaluate the frequency with which edible ice served in restaurants is contaminated with antibiotic-resistant bacteria, and thereby clarify whether this product poses a risk for ESBL-E carriage in humans. Ice from restaurants in Vietnam and Japan was screened for bacteria capable of growing on agar containing cefotaxime (BG-CTX)...
2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29040080/detection-of-giardia-intestinalis-in-water-samples-collected-from-natural-water-reservoirs-and-wells-in-northern-and-north-eastern-poland-using-lamp-real-time-pcr-and-nested-pcr
#18
Anna Lass, Beata Szostakowska, Krzysztof Korzeniewski, Panagiotis Karanis
Giardia intestinalis is a protozoan parasite, transmitted to humans and animals by the faecal-oral route, mainly through contaminated water and food. Knowledge about the distribution of this parasite in surface water in Poland is fragmentary and incomplete. Accordingly, 36 environmental water samples taken from surface water reservoirs and wells were collected in Pomerania and Warmia-Masuria provinces, Poland. The 50 L samples were filtered and subsequently analysed with three molecular detection methods: loop-mediated isothermal amplification (LAMP), real-time polymerase chain reaction (real-time PCR) and nested PCR...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29040078/genetic-diversity-of-escherichia-coli-isolates-from-surface-water-and-groundwater-in-a-rural-environment
#19
Maria Laura Gambero, Monica Blarasin, Susana Bettera, Jesica Giuliano Albo
The genetic characteristics among Escherichia coli strains can be grouped by origin of isolation. Then, it is possible to use the genotypes as a tool to determine the source of water contamination. The aim of this study was to define water aptitude for human consumption in a rural basin and to assess the diversity of E. coli water populations. Thus, it was possible to identify the main sources of fecal contamination and to explore linkages with the hydrogeological environment and land uses. The bacteriological analysis showed that more than 50% of samples were unfit for human consumption...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29039992/polymerase-chain-reaction-and-goldmann-witmer-coefficient-to-examine-the-role-of-epstein-barr-virus-in-uveitis
#20
Derrick Smit, David Meyer, Jean Maritz, Jolanda D F de Groot-Mijnes
PURPOSE: To use polymerase chain reaction (PCR) and Goldmann-Witmer Coefficient (GWC) calculation to search for evidence that Epstein-Barr virus (EBV) causes uveitis. METHODS: A prospective cross-sectional study where participants with positive multiplex EBV PCR results were further investigated by: 1) real-time PCR for EBV viral loads (VL) and 2) EBV GWC. RESULTS: Eleven of 106 consecutive uveitis patients (10.4%) had positive multiplex PCR for EBV on aqueous humor sampling and 7/11 (63...
October 17, 2017: Ocular Immunology and Inflammation
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