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Igm Monoclonal

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https://www.readbyqxmd.com/read/29219127/dengue-fever-in-a-municipality-of-west-bengal-india-2015-an-outbreak-investigation
#1
Falguni Debnath, Manickam Ponnaiah, Pralay Acharya
BACKGROUND: In November 2015, death due to fever and increased number of fever cases were reported from Baranagar Municipality of North 24 Parganas district of West Bengal. OBJECTIVES: The episode was investigated with the objective to (1) confirm the existence of an outbreak, (2) describe it in terms of time, place, and person, (3) determine the cause of outbreak, and (4) recommend control measures. METHODS: Monthly incidence of dengue from 2012 to 2014 was calculated and compared with 2015 to confirm the outbreak...
October 2017: Indian Journal of Public Health
https://www.readbyqxmd.com/read/29218495/systemic-epstein-barr-virus-positive-t-cell-lymphoproliferative-disorders-of-childhood-with-fulminant-leukocytosis-and-tumor-lysis-a-case-report-with-autopsy-findings
#2
Sachie Wada, Takayuki Suzuki, Koichi Kitazume, Akira Fujita, Seiichiro Shimizu
Systemic Epstein-Barr virus (EBV)-positive T-cell lymphoproliferative disorders (T-LPD) of childhood is an extremely rare disease characterized by an aggressive clinical course and very poor prognosis. We report an adolescent male with systemic EBV-positive T-LPD of childhood after primary EBV infection, resulting in a fatal clinical course within 9 days, along with autopsy findings. A 19-year-old male without an immunocompromised status presented with an acute onset of high fever, and was hospitalized for persistent fever, vomiting and diarrhea on the 5th day from onset...
December 7, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/29174537/flow-cytometry-based-method-for-rapid-and-high-throughput-screening-of-hybridoma-cells-secreting-monoclonal-antibody
#3
Sachi Akagi, Chika Nakajima, Yoichiro Tanaka, Yasuyuki Kurihara
Monoclonal antibodies (mAbs) are a valuable biomaterial for basic life sciences and industrial purposes. The production of the mAb is time and effort intensive. In this report, we established a time- and labor-saving method for the mAb production. Because membrane-type immunoglobulin on a hybridoma cell surface and its secreted form, called as antibody, share the same binding property to the antigen, the fluorescence-labeled antigen bound to membrane-type immunoglobulin can be used as a screening marker. In the method, a hybridoma labeled by a fluorescent antigen was selected and sorted singly into 96-well plate using flow cytometer...
November 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29169431/monoclonal-igm-gammopathy-and-waldenstr%C3%A3-m-s-macroglobulinemia
#4
Alexander Grunenberg, Christian Buske
BACKGROUND: 3.2-3.5% of persons over age 50 have a monoclonal gammopathy. Monoclonal gammopathies have many causes, including cancer. 10-20% of monoclonal gammopathies are of isotype IgM. A systematic approach to the differential diagnosis of IgM gammopathies is essential because of the different therapeutic implications of the various underlying conditions. METHODS: This review is based on pertinent publications retrieved by a selective search in PubMed and current guidelines from Germany and abroad...
November 3, 2017: Deutsches Ärzteblatt International
https://www.readbyqxmd.com/read/29159464/anti-mag-associated-cerebellar-ataxia-and-response-to-rituximab
#5
Panagiotis Zis, Dasappaiah Ganesh Rao, Nigel Hoggard, Ptolemaios Georgios Sarrigiannis, Marios Hadjivassiliou
BACKGROUND: Myelin-associated glycoprotein (MAG) is a glycoprotein specific to Schwann cells. Schwann cells produce myelin for nerve cells in the peripheral nervous system. MAG also plays a role in the central nervous system (CNS) by maintaining myelin integrity and inhibiting axonal regeneration from cerebellar neurons. There is a well-established link between distal demyelinating neuropathy and anti-MAG antibodies in patients with monoclonal gammopathy of unknown significance. We describe a series of five patients with anti-MAG antibodies with evidence of cerebellar rather than just sensory ataxia and our experience of treatment with rituximab...
November 20, 2017: Journal of Neurology
https://www.readbyqxmd.com/read/29153027/annals-express-large-abnormal-peak-on-capillary-zone-electrophoresis-due-to-contrast-agent
#6
Rachel D Wheeler, Liqun Zhang, Joanna Sheldon
BACKGROUND: Some iodinated radio-contrast media absorb UV light and can therefore be detected by capillary zone electrophoresis (CZE). If seen, these peaks are typically small with "quantifications" of below 5g/L. Here we describe detection of a large peak on CZE that was due to the radio-contrast agent, Omnipaque(T)(M). METHODS: Serum from a patient was analysed by CZE, and the IgG, IgA, IgM and total protein concentrations were measured. The serum sample was further analysed by gel electrophoresis and immunofixation...
January 1, 2017: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29121996/production-and-characterization-of-monoclonal-antibodies-against-encephalitozoon-intestinalis-and-encephalitozoon-sp-spores-and-their-developmental-stages
#7
Fernando Izquierdo, Hercules Moura, Fernando Jorge Bornay-Llinares, Rama Sriram, Carolina Hurtado, Ángela Magnet, Soledad Fenoy, Govinda Visvesvara, Carmen Del Aguila
BACKGROUND: Microsporidia are intracellular obligate parasites traditionally associated with immunosuppressed patients; their detection in immunocompetent patients has increased, highlighting their possible importance as emerging pathogens. Detection of spores in stools, urine, body fluids and tissues is difficult and immunological techniques such as immunofluorescence have proved to be a useful and reliable tool in the diagnosis of human microsporidiosis. For this reason, we have produced and characterized monoclonal antibodies (MAbs) specific for Encephalitozoon intestinalis (the second most frequent microsporidian infecting humans), and other Encephalitozoon species, that can be used in different diagnostic techniques...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29113857/a-rare-neurological-complication-of-waldenstrom-s-macroglobulinemia
#8
Gabriel Torrealba-Acosta, Rajan Gadhia, Thabele Leslie-Mazwi
Bilateral and simultaneous facial nerve palsy (FNP) is a rare clinical condition occurring in 0.3-2.0% of facial palsy cases and is typically a manifestation of an underlying systemic disease. We here describe a case of a 67-year-old Hispanic man with a known history of Waldenstrom's Macroglobulinemia (WM) who presented to the clinic with a sub-acute onset of bilateral facial weakness. No alternate etiology for the facial weakness was identified after a thorough diagnostic approach. WM is a rare hematological condition due to low-grade B cell lymphoma, where lymphoplasmacytoid cells infiltrate different tissues and secrete monoclonal IgM...
November 4, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/29105117/severe-mononeuritis-multiplex-after-rituximab-in-igm-%C3%AE%C2%BA-monoclonal-gammopathy
#9
Alexis A Lizarraga, Karlo J Lizarraga, James E Hoffman, Dana P Ascherman, Robert T Shebert, Ashok Verma
No abstract text is available yet for this article.
November 3, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/29101385/shotgun-glycomics-identifies-tumor-associated-glycan-ligands-bound-by-an-ovarian-carcinoma-specific-monoclonal-antibody
#10
B Liau, B Tan, G Teo, P Zhang, A Choo, P M Rudd
Cancers display distinctive carbohydrate molecules (glycans) on their surface proteins and lipids. mAb A4, an in-house generated monoclonal IgM antibody, is capable of distinguishing malignant ovarian carcinoma cells from benign ovarian epithelia by binding specifically to cancer cell-associated glycans. However, the structural details of the glycan targets of mAb A4 have been elusive. Here we developed a novel approach of isolating and fractionating glycan molecules released from glycoproteins in cancer cell lysates using HILIC-UPLC, and used them as probes on a microarray for affinity-based identification of the binding targets, allowing full-size, difficult to synthesize, cancer-associated glycans to be directly studied...
November 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29094629/infectious-diseases-and-immunological-markers-associated-with-patients-with-non-hodgkin-lymphoma-treated-with-rituximab
#11
Kleber Jordão de Souza, Rodrigo Sala Ferro, Luiz Euribel Prestes-Carneiro, Paula Andreia Martins Carrilho, Dewton de Moraes Vasconcelos
BACKGROUND: The use of rituximab (RTX) is increasing, even in developing countries. It has become the first-line therapy or adjuvant to chemotherapy (CHOP; cyclophosphamide, hydroxydaunorubicin, oncovin and prednisone) for various diseases, including B cell lymphoma and autoimmune diseases. AIM: We describe the infectious diseases and immunological markers associated with RTX treatment of patients with non-Hodgkin lymphoma (NHL). METHODS: Serum immunoglobulins were determined before and after intravenous immunoglobulin (IVIg) administration...
November 2, 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/29069762/cell-lines-generated-from-a-chronic-lymphocytic-leukemia-mouse-model-exhibit-constitutive-btk-and-akt-signaling
#12
Simar Pal Singh, Saravanan Y Pillai, Marjolein J W de Bruijn, Ralph Stadhouders, Odilia B J Corneth, Henk Jan van den Ham, Alice Muggen, Wilfred van IJcken, Erik Slinger, Annemieke Kuil, Marcel Spaargaren, Arnon P Kater, Anton W Langerak, Rudi W Hendriks
Chronic lymphocytic leukemia (CLL) is characterized by the accumulation of mature CD5(+) B cells in blood. Spontaneous apoptosis of CLL cells in vitro has hampered in-depth investigation of CLL pathogenesis. Here we describe the generation of three monoclonal mouse cell lines, EMC2, EMC4 and EMC6, from the IgH.TEμ CLL mouse model based on sporadic expression of SV40 large T antigen. The cell lines exhibit a stable CD5(+)CD43(+)IgM(+)CD19(+) CLL phenotype in culture and can be adoptively transferred into Rag1(-/-) mice...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29046148/exploring-the-unique-selectivity-of-hydrophobic-cation-exchanger-nuvia-cprime-for-the-removal-of-a-major-process-impurity-a-case-study-with-igm
#13
Xuemei M He, Carsten VoB, Jidong Li
Mixed-mode chromatography is becoming an important tool for downstream process purification, as it provides the selectivity and robustness unmatched by conventional single-mode chromatographic methods. The joint action of multiple functionalities present on the ligands of mixed-mode chromatography matrices effectively enhances the separation of target molecules from impurities. Using Nuvia cPrime as an example, we elucidate the separation principles of hydrophobic cation exchange mixed-mode chromatography and its difference from traditional strong cation exchangers...
October 17, 2017: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/29034435/advances-in-the-treatment-of-paraproteinemic-neuropathy
#14
REVIEW
Eduardo Nobile-Orazio, Mariangela Bianco, Andrea Nozza
Purpose of review Several advances have been made on the pathogenesis and therapy of neuropathies associated with paraproteinemia (monoclonal gammopathy). It is important for the neurologist to understand the pathogenetic relevance of this association especially when the hematological disease does not require per se any therapy. Recent findings Treatment of the neuropathy in patients with malignant paraproteinemia is mainly addressed by the hematologist while the neurologist is mainly involved in the initial diagnosis and in deciding whether the neuropathy is caused by the disease or by the chemotherapy used for the disease...
October 16, 2017: Current Treatment Options in Neurology
https://www.readbyqxmd.com/read/29027517/-unexpected-symptoms-of-monoclonal-gammopathy
#15
P M Smit, A C Abrahams, R G E Schutgens, N C Notermans, M C Minnema
Monoclonal gammopathy of undetermined significance (MGUS) is a common haematological disorder characterized by the presence of a monoclonal protein (M-protein). MGUS is considered an asymptomatic 'innocent' pre-malignant precursor condition of - mostly - multiple myeloma, without indication for treatment. We present three cases illustrating that MGUS can lead to serious problems. The first patient, a 51-year-old female, presented with polyneuropathy due to anti-MAG antibodies related to IgM MGUS. The second patient, a 37-year-old female, presented with proteinuria due to immunotactoid glomerulopathy caused by renal monoclonal IgG deposition associated with MGUS...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/29025291/-waldenstr%C3%A3-m-s-macroglobulinemia-and-its-individualized-therapy-options
#16
Zsuzsanna Szemlaky, Gábor Mikala
Waldenström's macroglobulinaemia is a form of lymphoplasmocytic lymphoma that preferentially localizes to the bone marrow and causes a special syndrome characterized by monoclonal IgM hypersecretion. Recent results point to the fact that this disease has at least three different pathobiological forms with different clinical presentation. While mutations of MYD88 occur in 95-97% of the cases, there are CXCR4 mutations in 30-40%, ARID1A mutations in 17% and CD79B mutations in approximately 10% of afflicted individuals...
October 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29021514/characteristics-and-prognosis-of-patients-with-immunoglobulin-m-monoclonal-gammopathy
#17
Hiroaki Tanaka, Yukie Sakuma, Hideki Ikeda, Ryo Shimizu, Yasumasa Sugita, Rie Iwai
Many patients with immunoglobulin M (IgM) monoclonal gammopathy remain asymptomatic and, consequently, untreated; however, few studies have evaluated the clinical course and prognosis of these patients. Using the screening procedures at our hospital, 74 patients with IgM monoclonal gammopathy were selected. We excluded 11 patients in whom the treatment for lymphoid neoplasms had been initiated at the time of IgM monoclonal protein detection. The remaining 63 patients were considered to be the patient population with IgM MGUS and asymptomatic WM, and were analyzed...
2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28988189/recurrent-chylothorax-a-clinical-mystery
#18
Eva Otoupalova, Shaiva Ginoya Meka, Sanjay Dogra, Bhavin Dalal
Chylothorax is an unusual cause of pleural effusion, typically caused by trauma or malignancy. Waldenstrom's macroglobulinaemia (WM) is a clinicopathological entity demonstrating lymphoplasmacytic lymphoma in the bone marrow with an IgM monoclonal gammopathy in the blood. Recurrent chylous effusions are often resistant to conservative treatment and may require surgical intervention. We present a unique case of a 50-year-old woman with recurrent chylothorax secondary to WM that completely resolved with ibrutinib therapy...
October 6, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28983055/pattern-of-somatic-mutations-in-patients-with-waldenstr%C3%A3-m-macroglobulinemia-or-igm-monoclonal-gammopathy-of-undetermined-significance
#19
Marzia Varettoni, Silvia Zibellini, Irene Defrancesco, Virginia Valeria Ferretti, Ettore Rizzo, Luca Malcovati, Anna Gallì, Matteo Giovanni Della Porta, Emanuela Boveri, Luca Arcaini, Chiara Candido, Marco Paulli, Mario Cazzola
We analyzed MYD88 and CXCR4 mutation status of 260 patients with Waldenström macroglobulinemia or IgM monoclonal gammopathy of undetermined significance using allele-specific real time quantitative PCR and Sanger sequencing respectively. A subgroup of 119 patients was further studied with next-generation sequencing of 11 target genes (MYD88, CXCR4, ARID1-A, KMT2D, NOTCH2, TP53, PRDM1, CD79B, TRAF3, MYBBP1A, TNFAIP3). MYD88 (L265P) was found at diagnosis in 91% of patients with Waldenström macroglobulinemia and in 60% of patients with IgM monoclonal gammopathy of undetermined significance using allele-specific PCR...
October 5, 2017: Haematologica
https://www.readbyqxmd.com/read/28979173/prevalence-of-rhd-variants-among-blood-donors-at-gulu-regional-blood-bank-gulu-northern-uganda
#20
Polycarp Ojok, Caesar Oyet, Fred Webbo, Bashir Mwambi, Ivan M Taremwa
AIM/OBJECTIVE: The aim of this study was to determine the prevalence of RhD variant phenotypes among voluntary non-remunerated blood donors (VNRBDs) at Gulu Regional Blood Bank (GRBB), Northern Uganda. MATERIALS AND METHODS: We conducted a cross-sectional study, in which the first 4.0 mL of ethylenediaminetetraacetic acid (EDTA) blood samples were collected from VNRBDs and typed for their ABO and RhD blood group status using IgM and IgG monoclonal typing antisera, respectively...
2017: Journal of Blood Medicine
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