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https://www.readbyqxmd.com/read/29458209/dermal-anti-oxidant-anti-inflammatory-and-anti-aging-effects-of-compritol-ato-based-resveratrol-colloidal-carriers-prepared-using-mixed-surfactants
#1
Haidy Abbas, Rabab Kamel, Nesrine El-Sayed
In this study, Compritol ATO-based Resveratrol colloidal carriers (CCCs) were prepared. In most formulae, the use of a binary-mixture of surfactants improved the physicochemical properties. CCC6 (containing P407/P188 as bi-surfactants) attained the highest drug loading, release efficiency during 24 hr and occlusive effect for 48 hr; in addition to, a uniform particle size distribution within the desired range. In-vivo studies were done based on the analysis of anti-oxidant markers [catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD)], anti-inflammatory markers [interleukin 6 (IL-6), interleukin 8 (IL-8) and rat Nuclear factor-kappa B (NF-κB)] and anti-wrinkling markers [matrix metalloproteinase (MMP-1) and Granulocyte-macrophage colony-stimulating factor (GM-CSF)], after UVB-irradiation...
February 16, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29458191/a-novel-neuropilin-1-binding-sequence-in-the-human-t-cell-lymphotropic-virus-type-1-envelope-glycoprotein
#2
Hideki Kusunoki, Toshiyuki Tanaka, Toshiyuki Kohno, Kazuhiko Matsuhashi, Kazuo Hosoda, Kaori Wakamatsu, Isao Hamaguchi
Entry of human T-cell lymphotropic virus type 1 (HTLV-1) into host cells is mainly mediated by interactions between the viral envelope glycoprotein surface unit (SU) and three host receptors: glucose transporter type 1, heparin/heparan sulfate proteoglycan, and neuropilin-1 (Nrp1). Here, we analyzed the interaction between HTLV-1 SU and Nrp1 using nuclear magnetic resonance and isothermal titration calorimetry. We found that two SU peptides, residues 85-94 and residues 304-312, bound directly to the Nrp1 b1 domain with affinities of 7...
February 16, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29458173/deregulation-of-nrf2-are-signaling-pathway-causes-susceptibility-of-dystrophin-deficient-myotubes-to-menadione-induced-oxidative-stress
#3
Su Jin Choi, Hye Sun Kim
Duchenne muscular dystrophy (DMD) is an X chromosome-linked disorder caused by a mutation in the dystrophin gene. Many previous studies reported that the skeletal muscles of DMD patients were more susceptible to oxidative stress than those of healthy people. However, not much has been known about the responsible mechanism of the differential susceptibility. In this study, we established dystrophin knock-down (DysKD) cell lines by transfection of dystrophin shRNA lentiviral particles into C2 cells and found that DysKD myotubes are more vulnerable to menadione-induced oxidative stress than control myotubes...
February 16, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29458164/structure-dependent-induction-of-apoptosis-by-hepatotoxic-pyrrolizidine-alkaloids-in-the-human-hepatoma-cell-line-heparg-single-versus-repeated-exposure
#4
Julia Waizenegger, Albert Braeuning, Markus Templin, Alfonso Lampen, Stefanie Hessel-Pras
Pyrrolizidine alkaloids (PA) are secondary plant compounds. PA intoxication in humans causes severe acute and chronic hepatotoxicity. However, the molecular mechanisms of PA hepatotoxicity in humans are not well understood yet. Therefore, we investigated cell death parameters in human HepaRG cells following either single (24 h) or repeated dose treatment (14 d) with structurally different PA of the retronecine (echimidine, senecionine), heliotridine (heliotrine), and otonecine type (senkirkine). After 24 h of exposure only retronecine-type PA were cytotoxic in HepaRG cells and induced apoptosis indicated by a loss of membrane asymmetry, disruption of the mitochondrial membrane potential, and increased pro-caspase and PARP cleavage...
February 16, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29458089/structural-and-biophysical-insight-into-dual-site-binding-of-the-protoberberine-alkaloid-palmatine-to-parallel-g-quadruplex-dna-using-nmr-fluorescence-and-circular-dichroism-spectroscopy
#5
Kumar Padmapriya, Ritu Barthwal
Plant derived small molecules, which interact with and stabilize G-quadruplex DNA, act as inhibitors of telomere elongation and oncogene expression in humans. The inhibition of telomerase enzyme has immense potential since it is over expressed in most cancer cells. Interaction of palmatine, an antitumor alkaloid, to parallel G-quadruplex DNA, [d(TTGGGGT)]4 and [d(TTAGGGT)]4 , has been investigated using Nuclear Magnetic Resonance (NMR), fluorescence and Circular Dichroism (CD) spectroscopy. Titrations were monitored by1 H and31 P NMR spectra and solution structure of palmatine-[d(TTGGGGT)]4 complex was obtained by restrained Molecular Dynamics (rMD) simulations using distance restraints from 2D NOESY spectra...
February 16, 2018: Biochimie
https://www.readbyqxmd.com/read/29458070/the-epigenetic-alterations-of-endogenous-retroelements-in-aging
#6
REVIEW
Maurizio Cardelli
Endogenous retroelements, transposons that mobilize through RNA intermediates, include some of the most abundant repetitive sequences of the human genome, such as Alu and LINE-1 sequences, and human endogenous retroviruses. Recent discoveries demonstrate that these mobile genetic elements not only act as intragenomic parasites, but also exert regulatory roles in living cells. The risk of genomic instability represented by endogenous retroelements is normally counteracted by a series of epigenetic control mechanisms which include, among the most important, CpG DNA methylation...
February 16, 2018: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/29458054/comparative-investigation-of-binding-interactions-with-three-steroidal-derivatives-of-d-gggt-4-g-quadruplex-aptamer
#7
Shan Huang, Yu Liang, Jianguo Cui, Jiangning Xie, Yi Liu, Baoqing Hu, Qi Xiao
Steroidal derivatives have attracted tremendous attentions in biological and biomedical areas, due to their variety biological activities. The investigation of structural influences helps in understanding their biological activities. The interactions of steroidal derivatives with DNA may play important roles in biological activities, however only a few investigations were reported on this issue. Herein, the structural influences of three steroidal derivatives were investigated based on their binding interactions with d(GGGT)4 G-quadruplex aptamer by spectroscopic approaches, nuclear magnetic resonance (NMR), electrochemical methods, and molecular modeling techniques...
February 16, 2018: Steroids
https://www.readbyqxmd.com/read/29458048/4-phenylbutyric-acid-protects-against-lipopolysaccharide-induced-bone-loss-by-modulating-autophagy-in-osteoclasts
#8
Hyun-Jung Park, Ho-Jung Son, Ok-Joo Sul, Jae-Hee Suh, Hye-Seon Choi
4-Phenylbutyric acid (4-PBA) has been used clinically to treat urea cycle disorders and is known to be an inhibitor of endoplasmic reticulum (ER) stress. We hypothesized that 4-PBA attenuates inflammatory bone loss by inducing autophagy, a process that is frequently accompanied by ER stress. Micro computerized tomography analysis showed that 4-PBA attenuated LPS-induced bone loss in mice. The increased area of TRAP-positive osteoclasts (OCs) and serum level of collagen type I fragments in lipopolysaccharide (LPS)-treated mice were also decreased when 4-PBA was administered, suggesting a protective role of 4-PBA in OCs...
February 16, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29457990/learning-from-fukushima-nuclear-power-in-east-asia
#9
Frank Boulton
No abstract text is available yet for this article.
February 19, 2018: Medicine, Conflict, and Survival
https://www.readbyqxmd.com/read/29457983/antimycobacterial-effect-of-ifng-interferon-gamma-induced-autophagy-is-dependent-on-the-hmox1-heme-oxygenase-1-mediated-increase-in-the-intracellular-calcium-levels-and-modulation-of-ppp3-calcineurin-tfeb-transcription-factor-eb-axis
#10
Nisha Singh, Pallavi Kansal, Zeeshan Ahmad, Naveen Baid, Hariom Kushwaha, Neeraj Khatri, Ashwani Kumar
IFNG (interferon gamma)-induced autophagy plays an important role in the elimination of intracellular pathogens, such as Mycobacterium tuberculosis (Mtb). However, the signaling cascade that leads to the increase in autophagy flux in response to IFNG is poorly defined. Here, we demonstrate that HMOX1 (heme oxygenase 1)-generated carbon monoxide (CO) is required for the induction of autophagy and killing of Mtb residing in macrophages in response to immunomodulation by IFNG. Interestingly, IFNG exposure of macrophages induces an increase in intracellular calcium levels that is dependent on HMOX1 generated CO...
February 19, 2018: Autophagy
https://www.readbyqxmd.com/read/29457853/mesenchymal-epithelial-transition-of-pancreatic-cancer-cells-at-perineural-invasion-sites-is-induced-by-schwann-cells
#11
Yoko Fujii-Nishimura, Ken Yamazaki, Yohei Masugi, Junya Douguchi, Yutaka Kurebayashi, Naoto Kubota, Hidenori Ojima, Minoru Kitago, Masahiro Shinoda, Akinori Hashiguchi, Michiie Sakamoto
Epithelial-mesenchymal transition (EMT) promotes invasion and metastasis of pancreatic ductal adenocarcinoma (PDAC). However, the importance of its reverse process, mesenchymal-epithelial transition (MET), for PDAC remains unclear. We aimed to characterize the histological finding "focal differentiation" in PDAC at perineural invasion sites in the context of MET and to investigate the role of Schwann cells in inducing tumor MET. Tumor differentiation and immunohistochemical expressions of E-cadherin, SMAD3, and vimentin at perineural invasion sites were examined in 168 PDAC tissues...
February 19, 2018: Pathology International
https://www.readbyqxmd.com/read/29457851/suppression-of-microrna-144-3p-attenuates-oxygen-glucose-deprivation-reoxygenation-induced-neuronal-injury-by-promoting-brg1-nrf2-are-signaling
#12
Yanru Li, Yongli Zhao, Mingkun Cheng, Yingjie Qiao, Yongtao Wang, Wancheng Xiong, Wei Yue
Accumulating evidence has reported that microRNA-144-3p (miR-144-3p) is highly related to oxidative stress and apoptosis. However, little is known regarding its role in cerebral ischemia/reperfusion-induced neuronal injury. Herein, our results showed that miR-144-3p expression was significantly downregulated in neurons following oxygen-glucose deprivation and reoxygenation (OGD/R) treatment. Overexpression of miR-144-3p markedly reduced cell viability, promoted cell apoptosis, and increased oxidative stress in neurons with OGD/R treatment, whereas downregulation of miR-144-3p protected neurons against OGD/R-induced injury...
February 19, 2018: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/29457836/deptor-suppresses-lipogenesis-and-ameliorates-hepatic-steatosis-and-acute-on-chronic-liver-injury-in-alcoholic-liver-disease
#13
Hanqing Chen, Feng Shen, Alex Sherban, Allison Nocon, Yu Li, Hua Wang, Ming-Jiang Xu, Xianliang Rui, Jinyan Han, Bingbing Jiang, Donghwan Lee, Na Li, Farnaz Keyhani-Nejad, Jian-Gao Fan, Feng Liu, Amrita Kamat, Nicolas Musi, Leonard Guarente, Pal Pacher, Bin Gao, Mengwei Zang
Alcoholic liver disease (ALD) is characterized by lipid accumulation and liver injury. However, how chronic alcohol consumption causes hepatic lipid accumulation remains elusive. The present study demonstrates that activation of the mechanistic target of rapamycin complex 1 (mTORC1) plays a causal role in alcoholic steatosis, inflammation and liver injury. Chronic-plus-binge ethanol feeding led to hyperactivation of mTORC1, as evidenced by increased phosphorylation of mTOR and its downstream kinase S6K1 in hepatocytes...
February 19, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29457713/probing-structural-changes-among-analogous-inhibitor-bound-forms-of-hiv-1-protease-and-a-drug-resistant-mutant-in-solution-by-nuclear-magnetic-resonance
#14
Shahid N Khan, John D Persons, Janet L Paulsen, Michel Guerrero, Celia A Schiffer, Nese Kurt-Yilmaz, Rieko Ishima
In the era of state-of-the-art inhibitor design and high-resolution structural studies, detection of significant but small protein structural differences in the inhibitor-bound forms is critical to further developing the inhibitor. Here, we probed differences in HIV-1 protease (PR) conformation among darunavir and four analogous inhibitor-bound forms and compared them with a drug-resistant mutant using nuclear magnetic resonance chemical shifts. Changes in amide chemical shifts of wild-type (WT) PR among these inhibitor-bound forms, ΔCSP, were subtle but detectable and extended >10 Å from the inhibitor-binding site, asymmetrically between the two subunits of PR...
February 19, 2018: Biochemistry
https://www.readbyqxmd.com/read/29457685/tailored-microstructured-hyperpolarizing-matrices-for-optimal-magnetic-resonance-imaging
#15
Matthieu Cavaillès, Aurélien Bornet, Xavier Jaurand, Basile Vuichoud, David Baudouin, Mathieu Baudin, Laurent Veyre, Geoffrey Baudenhausen, Jean-Nicolas Dumez, Sami Jannin, Christophe Copéret, Chloé Thieuleux
Tailoring the physical features and the porous network architecture of silica-based hyperpolarizing solids containing TEMPO radicals, known as HYPSO (HYbrid Polarizing SOlids), enable unprecedented performance of dissolution Dynamic Nuclear Polarization (d-DNP). High polarization values up to P(1H) = 99 % were reached for samples impregnated with a mixture of H2O:D2O and loaded in a 6.7 T polarizer at temperatures around 1.2 K. These HYPSO materials combine the best performance of homogeneous DNP formulations with the advantages of solid polarizing matrices which provide hyperpolarized solutions free of any - potentially toxic - additives (radicals and glass-forming agents)...
February 19, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29457364/transmission-electron-microscopy-analysis-of-the-origin-and-incidence-of-sperm-intranuclear-cytoplasmic-retention-in-fertile-and-teratozoospermia-men
#16
W-J Zhu
The human sperm nucleus contains cytoplasm. However, the origin and incidence of human sperm intranuclear cytoplasmic retention (INCR) remain unknown. The objectives of this study were to observe the morphological origin of INCR within the seminiferous epithelium and investigate the incidence of INCR in fertile and teratozoospermia men using transmission electron microscopy (TEM). By TEM, INCR initially appeared in elongating round spermatid nuclei and varied in size, number, shape, content, location and distribution within sperm nuclei...
February 18, 2018: Andrology
https://www.readbyqxmd.com/read/29457254/primordial-odontogenic-tumour-report-of-two-cases
#17
Asma Almazyad, Chia-Cheng Li, Roberto Onner Cruz Tapia, Javier Portilla Robertson, David Collette, Sook-Bin Woo
Primordial odontogenic tumour (POT) is a rare mixed odontogenic neoplasm that is composed of primitive ecto-mesenchyme resembling dental papilla, surfaced by odontogenic epithelium resembling inner enamel epithelium, without hard tissue formation. Most reported cases have presented in the posterior mandible as a well-demarcated radiolucency associated with an unerupted tooth in the first two decades of life. AIM: To describe the clinico-pathological features of two more cases of POT. METHODS AND RESULTS: Each presented as an asymptomatic well-delineated radiolucency in the mandible in a 15-year-old female and an 18-year-old male respectively...
February 19, 2018: Histopathology
https://www.readbyqxmd.com/read/29457159/gadolinium-based-endohedral-metallofullerene-gd-2-c-79-n-as-a-relaxation-boosting-agent-for-dissolution-dnp-at-high-fields
#18
Xiaoling Wang, Johannes E McKay, Bimala Lama, Johan van Tol, Tinghui Li, Kyle Kirkpatrick, Zhehong Gan, Stephen Hill, Joanna R Long, Harry C Dorn
We show increased dynamic nuclear polarization by adding a low dosage of a S = 15/2 Gd based endohedral metallofullerene (EMF) to DNP samples. By adding 60 μM Gd2 @C79 N, the nuclear polarization of1 H and13 C spins from 40 mM 4-oxo-TEMPO increases by approximately 40% and 50%, respectively, at 5 T and 1.2 K. Electron-electron double resonance (ELDOR) measurements show that the high spin EMF shortens the electron relaxation times and increases electron spectral diffusion leading to the increased DNP enhancement...
February 19, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29457048/farnesoid-x-receptor-expression-in-microscopic-colitis-a-potential-role-in-disease-etiopathogenesis
#19
Joana Torres, Carolina Palmela, Pedro Gomes de Sena, Maria Pia Costa Santos, Catarina Gouveia, Maria Helena Oliveira, Ana Raquel Henriques, Cecília Rodrigues, Marília Cravo, Paula Borralho
Introduction: Microscopic colitis (MC) is a chronic inflammatory bowel disease with unclear etiology. Bile acid (BA) malabsorption has been described in MC patients. Farnesoid X receptor (FXR) is the main BA receptor; FXR-mediated mechanisms prevent the noxious effects of BA accumulation, preserving the integrity of the intestinal epithelial barrier and preventing intestinal inflammation. Aim: Our aim was to describe the expression of FXR in patients with MC. Methods: Archival formalin-fixed paraffin-embedded samples from the terminal ileum, right and left colon were obtained from patients with MC and matched controls...
January 2018: GE Portuguese Journal of Gastroenterology
https://www.readbyqxmd.com/read/29456966/metabolic-profile-of-oral-squamous-carcinoma-cell-lines-relies-on-a-higher-demand-of-lipid-metabolism-in-metastatic-cells
#20
Ana Carolina B Sant'Anna-Silva, Gilson C Santos, Samir P Costa Campos, André Marco Oliveira Gomes, Juan Alberto Pérez-Valencia, Franklin David Rumjanek
Tumor cells are subjected to a broad range of selective pressures. As a result of the imposed stress, subpopulations of surviving cells exhibit individual biochemical phenotypes that reflect metabolic reprograming. The present work aimed at investigating metabolic parameters of cells displaying increasing degrees of metastatic potential. The metabolites present in cell extracts fraction of tongue fibroblasts and of cell lines derived from human tongue squamous cell carcinoma lineages displaying increasing metastatic potential (SCC9 ZsG, LN1 and LN2) were analyzed by1 H NMR (nuclear magnetic resonance) spectroscopy...
2018: Frontiers in Oncology
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