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https://www.readbyqxmd.com/read/29045935/emodinol-ameliorates-urate-nephropathy-by-regulating-renal-organic-ion-transporters-and-inhibiting-immune-inflammatory-responses-in-rats
#1
Hui Wu, Mengze Zhou, Guo Lu, Zhonglin Yang, Hui Ji, Qinghua Hu
Emodinol, 1β, 3β, 23-trihydroxyolean-12-en-28-acid, as the main active ingredient firstly extracted from the rhizomes of Elaeagus pungens by our Research Group, was identified with apparent uricosuric and nephroprotective effects in hyperuricemia mice in our previous study. This study aimed to investigate the renal protective effect of emodinol in urate nephropathy rats. Rats were orally administrated by combined adenine and ethambutol to induce urate nephropathy. Emodinol at various doses were administered intragastrically to urate nephropathy rats daily...
October 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29045922/the-impact-of-expired-commercial-drugs-on-non-target-marine-species-a-case-study-with-the-use-of-a-battery-of-biomarkers-in-hemocytes-of-mussels
#2
Nektarios Politakis, Alexia Belavgeni, Ioanna Efthimiou, Nikolina Charalampous, Chara Kourkouta, Stefanos Dailianis
The present study investigated the effects of two expired commercial medicines, like Buscopan Plus and Mesulid, commonly classified as household medical wastes, on hemocytes of mussel Mytilus galloprovincialis. Mussel hemocytes' lysosomal membrane stability (in terms of neutral red retention assay), superoxide anions (O2(·)(-)) and nitric oxides (NO, in terms of nitrites) production, lipid peroxidation (in terms of malondialdehyde/MDA content) and the formation of nuclear abnormalities (using the micronucleus/MN assay) were assessed in hemocytes of mussels treated for 7 days with appropriate amounts of each drug (the concentrations of active substances were considered in each case, due to the absence of data related with the excipients) as well as in hemocytes of post-treated/recovered mussels (7 days post-treatment/recovery period)...
October 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29045918/cervical-cancer-cells-hela-response-to-photodynamic-therapy-using-a-zinc-phthalocyanine-photosensitizer
#3
Natasha Hodgkinson, Cherie Ann Kruger, Mpho Mokwena, Heidi Abrahamse
Cervical cancer is the most common gynecological malignancy worldwide, and the leading cause of cancer related deaths among females. Conventional treatment for early cervical cancer is radical hysterectomy. In locally advanced cancer the treatment of choice is concurrent chemo radiation. Although such treatment methods show promise, they do have adverse side effects. To minimize these effects, as well as prevent cancer re-occurrence, new treatment methods are being investigated. Photodynamic therapy (PDT) involves the selective uptake of a photosensitizer (PS) by cancer cells, illumination with light of an appropriate wavelength that triggers a photochemical reaction leading to the generation of reactive oxygen and subsequent tumor regression...
October 4, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29045903/hpari-protein-secreted-by-a-helminth-parasite-suppresses-interleukin-33
#4
Megan Osbourn, Dinesh C Soares, Francesco Vacca, E Suzanne Cohen, Ian C Scott, William F Gregory, Danielle J Smyth, Matilda Toivakka, Andrea M Kemter, Thierry le Bihan, Martin Wear, Dennis Hoving, Kara J Filbey, James P Hewitson, Holly Henderson, Andrea Gonzàlez-Cìscar, Claire Errington, Sonja Vermeren, Anne L Astier, William A Wallace, Jürgen Schwarze, Alasdair C Ivens, Rick M Maizels, Henry J McSorley
Infection by helminth parasites is associated with amelioration of allergic reactivity, but mechanistic insights into this association are lacking. Products secreted by the mouse parasite Heligmosomoides polygyrus suppress type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. Here, we identified H. polygyrus Alarmin Release Inhibitor (HpARI), an IL-33-suppressive 26-kDa protein, containing three predicted complement control protein (CCP) modules. In vivo, recombinant HpARI abrogated IL-33, group 2 innate lymphoid cell (ILC2) and eosinophilic responses to Alternaria allergen administration, and diminished eosinophilic responses to Nippostrongylus brasiliensis, increasing parasite burden...
October 17, 2017: Immunity
https://www.readbyqxmd.com/read/29045828/prolonged-ikk%C3%AE-inhibition-improves-ongoing-ctl-antitumor-responses-by-incapacitating-regulatory-t-cells
#5
Christoph Heuser, Janine Gotot, Eveline Christina Piotrowski, Marie-Sophie Philipp, Christina Johanna Felicia Courrèges, Martin Sylvester Otte, Linlin Guo, Jonathan Leo Schmid-Burgk, Veit Hornung, Annkristin Heine, Percy Alexander Knolle, Natalio Garbi, Edgar Serfling, César Evaristo, Friedrich Thaiss, Christian Kurts
Regulatory T cells (Tregs) prevent autoimmunity but limit antitumor immunity. The canonical NF-κB signaling pathway both activates immunity and promotes thymic Treg development. Here, we report that mature Tregs continue to require NF-κB signaling through IκB-kinase β (IKKβ) after thymic egress. Mice lacking IKKβ in mature Tregs developed scurfy-like immunopathology due to death of peripheral FoxP3(+) Tregs. Also, pharmacological IKKβ inhibition reduced Treg numbers in the circulation by ∼50% and downregulated FoxP3 and CD25 expression and STAT5 phosphorylation...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045752/genome-size-and-sensitivity-to-dna-damage-by-x-rays-plant-comets-tell-the-story
#6
John Einset, Andrew R Collins
Among several factors affecting radiation sensitivity, genome size has received limited attention during the last 50 years since research at Brookhaven National Laboratory (USA) and other locations demonstrated substantial differences in radiation sensitivities, e.g. between tree species with large (e.g. conifers such as pines) versus small (e.g. dicots such as oaks) genome sizes. Taking advantage of the wide range of genome sizes among species, we investigated radiation sensitivity which we define in this study as DNA damage (break frequency) measured with the alkaline comet assay in isolated nuclei exposed to X-rays...
October 17, 2017: Mutagenesis
https://www.readbyqxmd.com/read/29045658/exendin-4-a-glucagon-like-peptide-1-receptor-agonist-attenuates-breast-cancer-growth-by-inhibiting-nf-%C3%AE%C2%BAb-activation
#7
Chikayo Iwaya, Takashi Nomiyama, Shiho Komatsu, Takako Kawanami, Yoko Tsutsumi, Yuriko Hamaguchi, Tsuyoshi Horikawa, Yasuteru Yoshinaga, Shinichi Yamashita, Tomoko Tanaka, Yuichi Terawaki, Makito Tanabe, Kazuki Nabeshima, Akinori Iwasaki, Toshihiko Yanase
Incretin therapies have received significant attention because of their tissue-protective effects, which extend beyond those associated with glycemic control. Cancer is a primary cause of death in patients with diabetes mellitus. We previously reported anti-prostate cancer effects of the glucagon-like peptide-1 (GLP-1) receptor (GLP-1R) agonist exendin-4 (Ex-4). Breast cancer is one of the most common cancers in female patients with type 2 diabetes mellitus and obesity. Thus, we examined whether GLP-1 action could attenuate breast cancer...
October 13, 2017: Endocrinology
https://www.readbyqxmd.com/read/29045639/a-new-perspective-on-polyploid-fragaria-strawberry-genome-composition-based-on-large-scale-multi-locus-phylogenetic-analysis
#8
Yilong Yang, Thomas M Davis
The subgenomic compositions of the octoploid (2n=8x=56) strawberry (Fragaria) species, including the economically important cultivated species Fragaria x ananassa, have been a topic of long-standing interest. Phylogenomic approaches utilizing next generation sequencing (NGS) technologies offer a new window into species relationships and the subgenomic compositions of polyploids. We have conducted a large scale phylogenetic analyses of Fragaria (strawberry) species using the Fluidigm Access Array system and 454 sequencing platform...
October 16, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29045617/purification-of-four-flavonoid-glycosides-from-lotus-nelumbo-nucifera-gaertn-plumule-by-macroporous-resin-combined-with-hsccc
#9
Lei Wu, Wei Xiong, Ju-Wu Hu, Zhen Gu, Jian-Guo Xu, Chuan-Ling Si, Young-Soo Bae, Gang Xu
High-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column was successfully applied to the isolation and purification of four flavonoid glycosides from the medicinal herb Lotus plumule (LP). A polar two-phase solvent system composed of ethyl acetate-n-butanol-water (1:2:3, v/v/v) was selected by high-performance liquid chromatography (HPLC) and run on a preparative scale where the lower aqueous phase was used as the mobile phase with a head-to-tail elution mode. Quercetin-3-O-β-D-glucopyranoside (15 mg), isorhamnetin-3-O-β-D-glucopyranoside (13 mg), apigenin 6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside (18 mg) and apigenin 6,8-di-C-β-D-glucopyranoside (48 mg) were obtained in a one-step HSCCC separation from 240 mg of the sample...
October 17, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29045501/scaffold-dependent-histone-deacetylase-hdac-inhibitor-induced-re-equilibration-of-the-subcellular-localization-and-post-translational-modification-state-of-class-i-hdacs
#10
Thomas W Hanigan, Taha Y Taha, Shaimaa M Aboukhatwa, Jonna Frasor, Pavel A Petukhov
The mechanism of action of histone deacetylase inhibitors (HDACi) is mainly attributed to the inhibition of the deacetylase catalytic activity for their histone substrates. In this study, we analyzed the abundance of class I HDACs in the cytosolic, nuclear soluble and chromatin bound cellular fractions in breast cancer cells after HDACi treatment. We found that potent N-hydroxy propenamide-based HDACi induced a concentration dependent decrease in the HDAC1 associated with chromatin and a lasting concomitant increase in cytoplasmic HDAC1 while maintaining total protein expression...
2017: PloS One
https://www.readbyqxmd.com/read/29045488/alseodaphnopsis-a-new-genus-of-lauraceae-based-on-molecular-and-morphological-evidence
#11
Yue-Qing Mo, Lang Li, Jian-Wu Li, Jens G Rohwer, Hsi-Wen Li, Jie Li
An investigation of a questionable species of the genus Alseodaphne led to the discovery of a new genus Alseodaphnopsis H. W. Li & J. Li, gen. nov., separated from Alseodaphne Nees, and a new species Alseodaphnopsis ximengensis H. W. Li & J. Li, sp. nov., endemic to Yunnan province, China. This new species is characterized by having big, axillary, paniculate inflorescences, as well as large, subglobose fruits. Based on DNA sequence data from two gene regions (nuclear ribosomal ITS and LEAFY intron II), we investigate its phylogenetic position within the Persea group...
2017: PloS One
https://www.readbyqxmd.com/read/29045468/cordycepin-promotes-apoptosis-in-renal-carcinoma-cells-by-activating-the-mkk7-jnk-signaling-pathway-through-inhibition-of-c-flipl-expression
#12
In-Hu Hwang, Seung Yoon Oh, Hyun-Jin Jang, Eunbi Jo, Jong Cheon Joo, Kyung-Bok Lee, Hwa-Seung Yoo, Mi Young Lee, Soo Jung Park, Ik-Soon Jang
Cellular FLICE inhibitory protein (c-FLIP) is a key anti-apoptotic regulator that associates with the signaling complex downstream of NF-κB, negatively interfering with apoptotic signaling. The role of c-FLIP downregulation by negative regulation of NF-κB signaling during apoptosis is poorly understood. Here, we demonstrate that NF-κB-mediated c-FLIPL negatively regulates the JNK signaling pathway, and that cordycepin treatment of human renal cancer cells leads to apoptosis induction through c-FLIPL inhibition...
2017: PloS One
https://www.readbyqxmd.com/read/29045385/usp7-small-molecule-inhibitors-interfere-with-ubiquitin-binding
#13
Lorna Kategaya, Paola Di Lello, Lionel Rougé, Richard Pastor, Kevin R Clark, Jason Drummond, Tracy Kleinheinz, Eva Lin, John-Paul Upton, Sumit Prakash, Johanna Heideker, Mark McCleland, Maria Stella Ritorto, Dario R Alessi, Matthias Trost, Travis W Bainbridge, Michael C M Kwok, Taylur P Ma, Zachary Stiffler, Bradley Brasher, Yinyan Tang, Priyadarshini Jaishankar, Brian R Hearn, Adam R Renslo, Michelle R Arkin, Frederick Cohen, Kebing Yu, Frank Peale, Florian Gnad, Matthew T Chang, Christiaan Klijn, Elizabeth Blackwood, Scott E Martin, William F Forrest, James A Ernst, Chudi Ndubaku, Xiaojing Wang, Maureen H Beresini, Vickie Tsui, Carsten Schwerdtfeger, Robert A Blake, Jeremy Murray, Till Maurer, Ingrid E Wertz
The ubiquitin system regulates essential cellular processes in eukaryotes. Ubiquitin is ligated to substrate proteins as monomers or chains and the topology of ubiquitin modifications regulates substrate interactions with specific proteins. Thus ubiquitination directs a variety of substrate fates including proteasomal degradation. Deubiquitinase enzymes cleave ubiquitin from substrates and are implicated in disease; for example, ubiquitin-specific protease-7 (USP7) regulates stability of the p53 tumour suppressor and other proteins critical for tumour cell survival...
October 18, 2017: Nature
https://www.readbyqxmd.com/read/29045336/bile-acid-receptors-and-the-kidney
#14
Michal Herman-Edelstein, Talia Weinstein, Moshe Levi
PURPOSE OF REVIEW: Bile acids act as activating signals of endogenous renal receptors: the nuclear receptor farnesoid X receptor (FXR) and the membrane-bound G protein-coupled bile acid receptor 1 (GPBAR1, also known as TGR5). In recent years, bile acids have emerged as important for renal pathophysiology by activating FXR and TGR5 and transcription factors relevant for lipid, cholesterol and carbohydrate metabolism, as well as genes involved in inflammation and renal fibrosis. RECENT FINDINGS: Activation of bile acid receptors has a promising therapeutic potential in prevention of diabetic nephropathy and obesity-induced renal damage, as well as in nephrosclerosis...
October 16, 2017: Current Opinion in Nephrology and Hypertension
https://www.readbyqxmd.com/read/29045235/the-aqueous-tuber-extract-of-pueraria-tuberosa-willd-d-c-caused-cytotoxic-effect-on-ht-29-cell-lines-with-down-regulation-of-nuclear-factor-kappa-b-nf-%C3%AE%C2%BAb
#15
Adeolu Alex Adedapo, Olusegun A Fagbohun, Christianah Dawurung, Ademola Adetokunbo Oyagbemi, Temidayo Olutayo Omobowale, Momoh Audu Yakubu
Background Pueraria tuberosa (Willd) D.C. (Fabaceae) tubers are already used in traditional medicine by Ayurvedic physicians for the management of fertility disorders, general weakness, and also as anti-ageing therapies. Other known pharmacological properties include: anti-hyperglycemics, hepatoprotective, anti-hyperlipidemic, diuretic, nutritive, and anti-fertility agents in male rats. Methods The anti-proliferative effect of the aqueous tuberous root extract of Pueraria tuberosa on vascular smooth muscle cells (VSMCs) and Human Colorectal Adenocarcinoma Cell lines (HT-29) was investigated using the Cell Titer 96 MTT Proliferation Assay where the viable cells were seeded at a density of 5 × 104 (100 µL/well)...
October 18, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/29045075/immunocytochemistry-for-sox-11-and-tfe3-as-diagnostic-markers-for-solid-pseudopapillary-neoplasms-of-the-pancreas-in-fna-biopsies
#16
Wen-Chi Foo, Grant Harrison, Xuefeng Zhang
BACKGROUND: Solid pseudopapillary neoplasms (SPNs) of the pancreas are rare malignant tumors that can be sampled via endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA). Although diagnosing SPNs can be straightforward in cases with a classic morphology and a typical immunoprofile, challenges can occur with morphologic variants or limited specimens. Recently, 2 immunohistochemical stains, SRY-related high-mobility group box 11 (SOX-11) and transcription factor E3 (TFE3), have been demonstrated to be highly sensitive and specific for SPNs in pancreatic resection specimens...
October 16, 2017: Cancer
https://www.readbyqxmd.com/read/29045036/endoplasmic-reticulum-stress-regulates-oxygen-glucose-deprivation-induced-parthanatos-in-human-sh-sy5y-cells-via-improvement-of-intracellular-ros
#17
Hai-Feng Wang, Zong-Qi Wang, Ye Ding, Mei-Hua Piao, Chun-Sheng Feng, Guang-Fan Chi, Yi-Nan Luo, Peng-Fei Ge
AIMS: Endoplasmic reticulum (ER) stress has been demonstrated to regulate neuronal death caused by ischemic insults via activation of apoptosis, but it still remains unclear whether ER stress participates in regulation of parthanatos, a new type of programmed cell death characterized by PARP-1 overactivation and intracellular accumulation of PAR polymer. METHODS: we used oxygen-glucose deprivation (OGD) and human SH-SY5Y cells to simulate neuronal damage caused by ischemia...
October 16, 2017: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/29044951/a-chemoenzymatic-histology-method-for-o-glcnac-detection
#18
Aime Lopez Aguilar, Xiaomeng Hou, Liuqing Wen, Peng George Wang, Peng Wu
Modification of nuclear and cytoplasmic proteins by the addition or removal of O-GlcNAc dynamically impacts multiple biological processes. Here we present the development of a chemoenzymatic histology method for the detection of O-GlcNAc in tissue samples. We applied this method to screen murine organs, uncovering specific O-GlcNAc distribution patterns in different tissue structures. We then utilized our histology method for O-GlcNAc detection in human brain samples from healthy donors and donors with Alzheimer's disease and found higher levels of O-GlcNAc in samples from healthy donors...
October 17, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29044939/synthesis-and-characterization-of-high-quantum-yield-and-oscillator-strength-6-chloro-2-4-cynophenyl-4-phenyl-quinoline-cl-cn-dpq-organic-phosphor-for-solid-state-lighting
#19
Minakshi Ghate, H K Dahule, N Thejo Kalyani, S J Dhoble
A novel blue luminescent 6-chloro-2-(4-cynophenyl) substituted diphenyl quinoline (Cl-CN DPQ) organic phosphor has been synthesized by the acid-catalyzed Friedlander reaction and then characterized to confirm structural, optical and thermal properties. Structural properties of Cl-CN-DPQ were analyzed by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR) spectroscopy, X-ray diffraction technique (XRD) and scanning electron microscopy (SEM) and energy dispersive analysis of X-ray (EDAX) spectroscopy...
October 18, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/29044928/a-rare-variant-rs933717-at-fbxo31-map1lc3b-in-chinese-is-associated-with-systemic-lupus-erythematosus
#20
Yuan-Yuan Qi, Xu-Jie Zhou, Swapan K Nath, Celi Sun, Yan-Na Wang, Ping Hou, Rong Mu, Chun Li, Jian-Ping Guo, Zhan-Guo Li, Geng Wang, Hu-Ji Xu, Yan-Jie Hao, Zhuo-Li Zhang, Wei-Hua Yue, Huo-Ru Zhang, Ming-Hui Zhao, Hong Zhang
OBJECTIVE: Recent evidence from genetic, cell biology and model animal studies have suggested a pivotal role of autophagy in mediating systemic lupus erythematosus (SLE). However, the genetic basis has not yet been thoroughly examined. The aim of the present study was therefore to identify additional susceptibility variants in autophagy-related genes, and their functional significance. METHODS: First, we performed a gene family-based genetic association analysis in patients with SLE using ImmunoChip, and selected the top-associated polymorphisms for replication in additional cohorts...
October 17, 2017: Arthritis & Rheumatology
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