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https://www.readbyqxmd.com/read/28432154/identification-of-the-septate-junction-protein-gliotactin-in-the-mosquito-aedes-aegypti-evidence-for-a-role-in-increased-paracellular-permeability-in-larvae
#1
Sima Jonusaite, Scott P Kelly, Andrew Donini
Septate junctions (SJs) regulate paracellular permeability across invertebrate epithelia. However, little is known about the function of SJ proteins in aquatic invertebrates. In this study, a role for the transmembrane SJ protein gliotactin (Gli) in the osmoregulatory strategies of larval mosquito (Aedes aegypti) was examined. Differences in gli transcript abundance were observed between the midgut, Malpighian tubules (MT), hindgut and anal papillae (AP) of A. aegypti, which are epithelia that participate in larval mosquito osmoregulation...
April 21, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28431328/effects-of-quantum-dots-on-the-ros-amount-of-liver-cancer-stem-cells
#2
Kunmeng Li, Chunhui Xia, Baiqi Wang, Hetao Chen, Tong Wang, Qian He, Hailong Cao, Yu Wang
Liver cancer (LC) is a serious disease that threatens human lives. LC has a high recurrence rate and poor prognosis. LC stem cells (LCSCs) play critical roles in these processes. However, the mechanism remains unclear. Reactive oxygen species (ROS) can be used to determine cell apoptosis and proliferation. However, studies of the effects of exogenous nanomaterials on LCSC ROS changes are rarely reported. In this work, quantum dots (QDs) were prepared using a hydrothermal method, and QDs were further modified with polyethylene glycol (PEG) and bovine serum albumin (BSA) using a chemical approach...
April 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28429764/fabrication-characterization-and-biological-evaluation-of-anti-her2-indocyanine-green-doxorubicin-encapsulated-peg-b-plga-copolymeric-nanoparticles-for-targeted-photochemotherapy-of-breast-cancer-cells
#3
Yu-Hsiang Lee, Da-Sheng Chang
In this study, we aimed to develop anti-human epidermal growth factor receptor 2 (HER2) indocyanine green (ICG)-doxorubicin (DOX)-encapsulated polyethylene glycol-poly(lactic-co-glycolic acid) diblock copolymeric nanoparticles (HIDPPNPs) to explore the co-administration of phototherapy and chemotherapy for HER2-overexpressing breast cancer, a highly aggressive and medicine-resistant breast carcinoma. The HIDPPNPs were fabricated using a solvent evaporation technique followed by carbodiimide-mediated antibody conjugation on the nanoparticle surface...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28428542/poly-protein-g-expressing-bacteria-enhance-the-sensitivity-of-immunoassays
#4
Wen-Rui Hao, Michael Chen, Yi-Jou Chen, Yu-Cheng Su, Chiu-Min Cheng, Hsiang-Yin Hsueh, An-Pei Kao, Yuan-Chin Hsieh, Johny Chang, Ming-Yang Tseng, Kuo-Hsiang Chuang
The sensitivities of solid-phase immunoassays are limited by the quantity of detection antibodies bound to their antigens on the solid phase. Here, we developed a poly-protein G-expressing bacterium as an antibody-trapping microparticle to enhance the signals of immunoassays by increasing the accumulation of detection antibodies on the given antigen. Eight tandemly repeated fragment crystallisable (Fc) binding domains of protein G were stably expressed on the surface of Escherichia coli BL21 cells (termed BL21/8G)...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28426936/cholesterol-assisted-bacterial-cell-surface-engineering-for-photodynamic-inactivation-of-gram-positive-and-gram-negative-bacteria
#5
Hao-Ran Jia, Ya-Xuan Zhu, Zhan Chen, Fu-Gen Wu
Antibacterial photodynamic therapy (PDT) which enables effective killing of regular and multidrug-resistant (MDR) bacteria is a promising treatment modality for bacterial infection. However, because most photosensitizer (PS) molecules fail to strongly interact with the surface of Gram-negative bacteria, this technique is only suitable for treating Gram-positive bacterial infection, which largely hampers its practical applications. Herein, we revealed for the first time that cholesterol could significantly facilitate the hydrophobic binding of PSs to the bacterial surface, achieving the hydrophobic interaction-based bacterial cell surface engineering that could effectively photo-inactivate both Gram-negative and Gram-positive bacteria...
April 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28426224/quantitative-analysis-of-polymer-brush-formation-kinetics-using-quartz-crystal-microbalance-viscoelasticity-of-polymer-brush
#6
Hirokazu Tanoue, Norifumi L Yamada, Kohzo Ito, Hideaki Yokoyama
Polymer brush formation kinetics was measured by quartz crystal microbalance (QCM). In the QCM measurement anomalous complex frequency shift as a function of graft density was observed: dissipation rate shift ΔΓ first increased, showed a peak and then decreased despite the graft density of the polymer brush was increasing monotonically. We calculated the shear modulus of the brush layer from the measured complex frequency shift and revealed that the peak of ΔΓ described the crossover from a viscous brush layer to elastic brush layer...
April 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28425752/use-of-standard-laboratory-methods-to-obviate-routine-dithiothreitol-treatment-of-blood-samples-with-daratumumab-interference
#7
Nicholas J Lintel, Debra K Brown, Diane T Schafer, Farai M Tsimba-Chitsva, Scott A Koepsell, Sara M Shunkwiler
Daratumumab is an antibody currently used in the treatment of patients with refractory multiple myeloma. Blood samples from patients being treated with daratumumab may show panreactivity during pre-transfusion testing. To facilitate the provision of blood components for such patients, it is recommended that a baseline phenotype or genotype be established prior to starting treatment with daratumumab. If patient red blood cells (RBCs) require phenotyping after the start of daratumumab treatment, dithiothreitol (DTT) treatment of the patient's RBCs should be performed...
January 2017: Immunohematology
https://www.readbyqxmd.com/read/28425323/enhanced-solubility-of-piperine-using-hydrophilic-carrier-based-potent-solid-dispersion-systems
#8
Thenmozhi Kathavarayan, Young Je Yoo
CONTEXT: Piperine alkaloid, an important constituent of black pepper exhibits numerous therapeutic properties whereas its usage as a drug is limited due to its poor solubility in aqueous medium, which leads to poor bioavailability. OBJECTIVE: Herein a new method has been developed to improve the solubility of this drug based on the development of solid dispersions with improved dissolution rate using hydrophilic carriers such as sorbitol (Sor), polyethylene glycol (PEG) and polyvinyl pyrrolidone K30 (PVP) by solvent method...
April 20, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28425308/the-effect-of-molecular-weights-of-microencapsulating-polymers-on-viability-of-mouse-cloned-pancreatic-%C3%AE-cells-biomaterials-osmotic-forces-and-potential-applications-in-diabetes-treatment
#9
Armin Mooranian, Ryu Tackechi, Emma Jamieson, Grant Morahan, Hani Al-Salami
INTRODUCTION: Ideal cell-containing microcapsules should be capable of maintaining cell viability and exhibit significant structural stability to support cellular functionality. To date, such microcapsules remain unavailable, thus, this study used our well-established microencapsulating methods to examine a total of 32 different microencapsulating formulations and correlate polymers' molecular weights (Mwt) and UDCA addition, with cell viability and microcapsules' stability, postmicroencapsulation...
April 20, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28425284/ph-triggered-size-increasing-gene-carrier-for-efficient-tumor-accumulation-and-excellent-anti-tumor-effect
#10
Zhaopei Guo, Jie Chen, Lin Lin, Xiuwen Guan, Pingjie Sun, Meiwan Chen, Huayu Tian, Xuesi Chen
High efficiency and well serum resistant capacity were very important for gene carrier in vivo usage. In this article, transfection efficiency and cell toxicity of polyethyleneimine (PEI) (branched, Mw=25K) was remarkably improved, when simply mixed polyanion (polyethylene glycol-polyglutamic acid (PEG-PLG) or polyglutamic acid (PLG)) with PEI. Different composites order of PEI, polyanion and gene (for example, PEI first complex with DNA, then complex with polyanion, or PEI first complex with polyanion, then complex with DNA) was studied and the result showed that only firstly forming polyanion/PEI complexes there would be some additional excellent properties, for example, pH decreasing induced particle size increasing, enhanced serum resistant ability and better tumor accumulation...
April 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28424334/glycosaminoglycan-based-hydrogels-capture-inflammatory-chemokines-and-rescue-defective-wound-healing-in-mice
#11
Nadine Lohmann, Lucas Schirmer, Passant Atallah, Elke Wandel, Ruben A Ferrer, Carsten Werner, Jan C Simon, Sandra Franz, Uwe Freudenberg
Excessive production of inflammatory chemokines can cause chronic inflammation and thus impair cutaneous wound healing. Capturing chemokine signals using wound dressing materials may offer powerful new treatment modalities for chronic wounds. Here, a modular hydrogel based on end-functionalized star-shaped polyethylene glycol (starPEG) and derivatives of the glycosaminoglycan (GAG) heparin was customized for maximal chemokine sequestration. The material is shown to effectively scavenge the inflammatory chemokines MCP-1 (monocyte chemoattractant protein-1), IL-8 (interleukin-8), and MIP-1α (macrophage inflammatory protein-1α) and MIP-1β (macrophage inflammatory protein-1β) in wound fluids from patients suffering from chronic venous leg ulcers and to reduce the migratory activity of human monocytes and polymorphonuclear neutrophils...
April 19, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28423917/management-of-opioid-induced-constipation-in-hospice-patients
#12
Leah Sera, Mary Lynn McPherson
BACKGROUND: Constipation is a common symptom in patients with advanced disease taking opioids. Opioid-induced constipation (OIC) is commonly treated with laxatives and stool softeners. Recently, newer agents have come to market which broaden options for patients in whom first-line therapies are not effective. OBJECTIVE: To determine what pharmacologic regimens are currently used in hospice programs to prevent and treat OIC, whether those regimens have changed with the introduction of newer agents and evidence discouraging the use of docusate, and whether hospice programs are standardizing the management of OIC...
January 1, 2017: American Journal of Hospice & Palliative Care
https://www.readbyqxmd.com/read/28423213/systemic-tumor-targeted-sodium-iodide-symporter-nis-gene-therapy-of-hepatocellular-carcinoma-mediated-by-b6-peptide-polyplexes
#13
Sarah Urnauer, Kathrin Klutz, Geoffrey K Grünwald, Stephan Morys, Nathalie Schwenk, Christian Zach, Franz-Josef Gildehaus, Wolfgang Roedl, Manfred Ogris, Ernst Wagner, Christine Spitzweg
BACKGROUND: Non-viral polymer-based gene transfer represents an adaptable system for tumor-targeted gene therapy, as various design strategies of shuttle systems together with the mechanistic concept of active tumor targeting lead to improved gene delivery vectors resulting in higher tumor specificity, efficacy and safety. METHODS: Using the sodium iodide symporter (NIS) as theranostic gene, non-viral gene delivery vehicles based on linear polyethylenimine (LPEI), polyethylene glycol (PEG) and coupled with the synthetic peptide B6 (LPEI-PEG-B6), which specifically binds to tumor cells, were investigated in a hepatocellular carcinoma (HCC) xenograft model for tumor-selectivity and transduction efficiency...
April 19, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28421968/chronic-toxicology-studies-of-basal-insulin-peglispro-in-rats-and-dogs-a-novel-pegylated-insulin-lispro-analog-with-a-prolonged-duration-of-action
#14
Richard A Byrd, Jamie L Blackbourne, Mary Pat Knadler, Albert E Schultze, John L Vahle
Basal insulin peglispro (BIL) consists of insulin lispro with a 20-kDa polyethylene glycol (PEG) moiety covalently attached to lysine B28. Because chronic parenteral administration of PEGylated proteins to animals has sometimes resulted in PEG vacuolation of tissue macrophages, renal tubular cells, and choroid plexus ependymal cells, we investigated whether chronic subcutaneous (sc) injection of BIL in rats (52 weeks) and dogs (39 weeks) was associated with systemic toxicities or other changes, including vacuolation of tissue macrophages, renal tubular cells, and ependymal cells...
April 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/28420299/surface-chemistry-of-gold-nanoparticles-determines-the-biocorona-composition-impacting-cellular-uptake-toxicity-and-gene-expression-profiles-in-human-endothelial-cells
#15
Parwathy Chandran, Jim E Riviere, Nancy A Monteiro-Riviere
This study investigated the role of nanoparticle size and surface chemistry on biocorona composition and its effect on uptake, toxicity and cellular responses in human umbilical vein endothelial cells (HUVEC), employing 40 and 80 nm gold nanoparticles (AuNP) with branched polyethyleneimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coatings. Proteomic analysis identified 59 hard corona proteins among the various AuNP, revealing largely surface chemistry-dependent signature adsorbomes exhibiting human serum albumin (HSA) abundance...
April 19, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28419961/evidence-towards-the-involvement-of-nitric-oxide-in-drought-tolerance-of-sugarcane
#16
Neidiquele M Silveira, John T Hancock, Lucas Frungillo, Eleni Siasou, Fernanda C C Marcos, Ione Salgado, Eduardo C Machado, Rafael V Ribeiro
Exogenous supply of nitric oxide (NO) increases drought tolerance in sugarcane plants. However, little is known about the role of NO produced by plants under water deficit. The aim of this study was to test the hypothesis that drought-tolerance in sugarcane is associated with NO production and metabolism, with the more drought-tolerant genotype presenting higher NO accumulation in plant tissues. The sugarcane genotypes IACSP95-5000 (drought-tolerant) and IACSP97-7065 (drought-sensitive) were submitted to water deficit by adding polyethylene glycol (PEG-8000) in nutrient solution to reduce the osmotic potential to -0...
April 8, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28419654/degradation-and-metabolism-of-synthetic-plastics-and-associated-products-by-pseudomonas-spp-capabilities-and-challenges
#17
REVIEW
Rebecca A Wilkes, Ludmilla Aristilde
Synthetic plastics, which are widely present in materials of everyday use, are ubiquitous and slow-degrading polymers in environmental wastes. Of special interest are the capabilities of microorganisms to accelerate their degradation. Members of the metabolically-diverse genus Pseudomonas are of particular interest due their capabilities to degrade and metabolize synthetic plastics. Pseudomonas species isolated from environmental matrices have been identified to degrade polyethylene, polypropylene, polyvinyl chloride, polystyrene, polyurethane, polyethylene terephthalate, polyethylene succinate, polyethylene glycol, and polyvinyl alcohol at varying degrees of efficiency...
April 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28418637/development-and-application-of-an-msall-based-approach-for-the-quantitative-analysis-of-linear-polyethylene-glycols-in-rat-plasma-by-liquid-chromatography-triple-quadrupole-time-of-flight-mass-spectrometry
#18
Xiaotong Zhou, Xiangjun Meng, Longmei Cheng, Chong Su, Yantong Sun, Lingxia Sun, Zhaohui Tang, John Paul Fawcett, Yan Yang, Jingkai Gu
Polyethylene glycols (PEGs) are synthetic polymers composed of repeating ethylene oxide subunits. They display excellent biocompatibility and are widely used as pharmaceutical excipients. To fully understand the biological fate of PEGs requires accurate and sensitive analytical methods for their quantitation. Application of conventional liquid chromatography-tandem mass spectrometry (LC-MS/MS) is difficult because PEGs have polydisperse molecular weights (MWs) and tend to produce multicharged ions in-source resulting in innumerable precursor ions...
April 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28417466/s-nitrosoglutathione-spraying-improves-stomatal-conductance-rubisco-activity-and-antioxidant-defense-in-both-leaves-and-roots-of-sugarcane-plants-under-water-deficit
#19
Neidiquele M Silveira, Fernanda C C Marcos, Lucas Frungillo, Bárbara B Moura, Amedea B Seabra, Ione Salgado, Eduardo C Machado, John T Hancock, Rafael V Ribeiro
Water deficit is a major environmental constraint on crop productivity and performance and nitric oxide (NO) is an important signaling molecule associated with many biochemical and physiological processes in plants under stressful conditions. This study aims to test the hypothesis that leaf spraying of S-nitrosoglutathione (GSNO), an NO donor, improves the antioxidant defense in both roots and leaves of sugarcane plants under water deficit, with positive consequences for photosynthesis. In addition, the role of key photosynthetic enzymes ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) in maintaining CO2 assimilation of GSNO-sprayed plants under water deficit were evaluated...
April 17, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28417193/genome-wide-analysis-of-vq-motif-containing-proteins-in-moso-bamboo-phyllostachys-edulis
#20
Yujiao Wang, Huanlong Liu, Dongyue Zhu, Yameng Gao, Hanwei Yan, Yan Xiang
29 Moso bamboo VQ proteins were genome-wide identified for the first time, and bioinformatics analysis was performed to investigate phylogenetic relationships and evolutionary divergence. The qRT-PCR data show that PeVQ genes response to different stress treatments. Accumulating evidence suggests that VQ motif-containing proteins in rice (Oryza sativa), Arabidopsis (Arabidopsis thaliana), and maize (Zea mays) play fundamental roles in response to various biotic and abiotic stresses. However, little is known about the functions of VQ family proteins in Moso bamboo (Phyllostachys edulis)...
April 17, 2017: Planta
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