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https://www.readbyqxmd.com/read/28453892/the-psycho-social-impact-of-contralateral-risk-reducing-mastectomy-crrm-on-women-a-rapid-review
#1
REVIEW
Karen Collins, Melanie Gee, Anna Clack, Lynda Wyld
OBJECTIVES: For women who have been diagnosed with unilateral breast cancer there is an increasing trend for them to request removal of the contralateral healthy breast, so called contralateral risk reducing mastectomy (CRRM). The current literature is only just beginning to identify patient-reported reasons for undergoing CRRM and associated patient reported outcomes. It is also unclear whether women at moderate/high risk of developing a subsequent primary contralateral breast cancer report similar outcomes to those considered to be at low/average risk...
April 28, 2017: Psycho-oncology
https://www.readbyqxmd.com/read/28453638/etg-ets-in-serum-by-uhplc-ms-ms-in-suspected-sexual-assault-cases
#2
Solfrid Hegstad, Arne Helland, Cecilie Hagemann, Olav Spigset
A method including semi-automated extraction of ethyl glucuronide (EtG) and ethyl sulfate (EtS) from serum followed by ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS-MS) has been developed and validated. Sample preparation prior to UHPLC-MS-MS analysis consisted of protein precipitation and filtration through a phospholipid removal plate. Chromatography was achieved using an HSS T3 column and gradient elution with formic acid in water in combination with methanol. The mass spectrometer was monitored in the negative mode with multiple reaction monitoring...
April 27, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28453559/evaluation-of-the-reproducibility-of-amplicon-sequencing-with-illumina-miseq-platform
#3
Chongqing Wen, Liyou Wu, Yujia Qin, Joy D Van Nostrand, Daliang Ning, Bo Sun, Kai Xue, Feifei Liu, Ye Deng, Yuting Liang, Jizhong Zhou
Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq. The V4 region of 16S rRNA gene from each sample was sequenced in triplicate with each replicate having a unique barcode. The average OTU overlap, without considering sequence abundance, at a rarefaction level of 10,323 sequences was 33...
2017: PloS One
https://www.readbyqxmd.com/read/28449270/strong-association-between-cardiorespiratory-fitness-and-serum-lipoprotein-subclass-pattern-in-prepubertal-healthy-children
#4
Geir Kåre Resaland, Tarja Rajalahti, Eivind Aadland, Olav Martin Kvalheim
AIM: To reveal the lipoprotein subclass pattern associated to cardiorespiratory fitness (CRF) in healthy children. METHODS: Serum concentrations of lipoprotein subclasses and concentrations and average particle size of their main classes were quantified in 94 ethnic Norwegian children using high performance liquid chromatography (HPLC). Twenty-four lipoprotein features were used as input to multivariate regression analysis with CRF measured either as peak oxygen consumption (VO2peak) using a continuous treadmill protocol or indirectly by the 10 minute Andersen intermittent running field test...
April 27, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28449031/rnablueprint-flexible-multiple-target-nucleic-acid-sequence-design
#5
Stefan Hammer, Birgit Tschiatschek, Christoph Flamm, Ivo L Hofacker, Sven Findeiß
Motivation: Realizing the value of synthetic biology in biotechnology and medicine requires the design of molecules with specialized functions. Due to its close structure to function relationship, and the availability of good structure prediction methods and energy models, RNA is perfectly suited to be synthetically engineered with predefined properties. However, currently available RNA design tools cannot be easily adapted to accommodate new design specifications. Furthermore, complicated sampling and optimization methods are often developed to suit a specific RNA design goal, adding to their inflexibility...
April 25, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28448954/copper-complexes-containing-thiosemicarbazones-derived-from-6-nitropiperonal-antimicrobial-and-biophysical-properties
#6
Floyd A Beckford, Kelsey R Webb
A series of four thiosemicarbazones from 6-nitropiperonal along with the corresponding copper complexes were synthesized. The biophysical characteristics of the complexes were investigated by the binding to DNA and human serum albumin. The binding to DNA is moderate; the binding constants run from (0.49-7.50)×10(4)M(-1). In relation to HSA, the complexes interact strongly with binding constants on the order of 10(5)M(-1). The complexes also display antioxidant behavior as determined by the ability to scavenge diphenylpicrylhydrazyl (dpph) and nitric oxide radicals...
April 20, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28448660/combining-local-and-global-limitations-of-visual-search
#7
Endel Põder
There are different opinions about the roles of local interactions and central processing capacity in visual search. This study attempts to clarify the problem using a new version of relevant set cueing. A central precue indicates two symmetrical segments (that may contain a target object) within a circular array of objects presented briefly around the fixation point. The number of objects in the relevant segments, and density of objects in the array were varied independently. Three types of search experiments were run: (a) search for a simple visual feature (color, size, and orientation); (b) conjunctions of simple features; and (c) spatial configuration of simple features (rotated Ts)...
April 1, 2017: Journal of Vision
https://www.readbyqxmd.com/read/28448626/genetic-background-dependent-effects-of-murine-micro-rnas-on-circadian-clock-function
#8
Silke Kiessling, Ahmet Ucar, Kamal Chowdhury, Henrik Oster, Gregor Eichele
MicroRNAs (miRs) are important regulators of a wide range of biological processes. Antagomir studies suggest an implication of miR-132 in the functionality of the mammalian circadian clock. miR-212 and miR-132 are tandemly processed from the same transcript and share the same seed region. We found the clock modulator miR-132 and miR-212 to be expressed rhythmically in the central circadian clock. Consequently, mRNAs implicated in circadian functions may likely be targeted by both miRs. To further characterize the circadian role we generated mice with stable deletion of the miR-132/212 locus and compared the circadian behavior of mutant and wild-type control animals on two genetic backgrounds frequently used in chronobiological research, C57BL/6N and 129/Sv...
2017: PloS One
https://www.readbyqxmd.com/read/28448575/speeding-up-profiling-program-s-runtime-characteristics-for-workload-consolidation
#9
Lin Wang, Depei Qian, Zhongzhi Luan, Guang Wei, Rui Wang, Hailong Yang
Workload consolidation is a common method to increase resource utilization of the clusters or data centers while still trying to ensure the performance of the workloads. In order to get the maximum benefit from workload consolidation, the task scheduler has to understand the runtime characteristics of the individual program and schedule the programs with less resource conflict onto the same server. We propose a set of metrics to comprehensively depict the runtime characteristics of programs. The metrics set consists of two types of metrics: resource usage and resource sensitivity...
2017: PloS One
https://www.readbyqxmd.com/read/28448556/a-highly-specific-and-sensitive-massive-parallel-sequencer-based-test-for-somatic-mutations-in-non-small-cell-lung-cancer
#10
Yoshiaki Inoue, Jun Shiihara, Hitoshi Miyazawa, Hiromitsu Ohta, Megumi Higo, Yoshiaki Nagai, Kunihiko Kobayashi, Yasuo Saijo, Masanori Tsuchida, Mitsuo Nakayama, Koichi Hagiwara
Molecular targeting therapy for non-small cell lung cancer (NSCLC) has clarified the importance of mutation testing when selecting treatment regimens. As a result, multiple-gene mutation tests are urgently needed. We developed a next-generation sequencer (NGS)-based, multi-gene test named the MINtS for investigating driver mutations in both cytological specimens and snap-frozen tissue samples. The MINtS was used to investigate the EGFR, KRAS, BRAF genes from DNA, and the ERBB2, and the ALK, ROS1, and RET fusion genes from RNA...
2017: PloS One
https://www.readbyqxmd.com/read/28448506/the-dynamics-of-behavior-in-modified-dictator-games
#11
Jeannette Brosig-Koch, Thomas Riechmann, Joachim Weimann
We investigate the dynamics of individual pro-social behavior over time. The dynamics are tested by running the same experiment with the same subjects at several points in time. To exclude learning and reputation building, we employ non-strategic decision tasks and a sequential prisoners-dilemma as a control treatment. In the first wave, pro-social concerns explain a high share of individual decisions. Pro-social decisions decrease over time, however. In the final wave, most decisions can be accounted for by assuming pure selfishness...
2017: PloS One
https://www.readbyqxmd.com/read/28448485/assessment-of-engineered-cells-using-cellnet-and-rna-seq
#12
Arthur H Radley, Remy M Schwab, Yuqi Tan, Jeesoo Kim, Emily K W Lo, Patrick Cahan
CellNet is a computational platform designed to assess cell populations engineered by either directed differentiation of pluripotent stem cells (PSCs) or direct conversion, and to suggest specific hypotheses to improve cell fate engineering protocols. CellNet takes as input gene expression data and compares them with large data sets of normal expression profiles compiled from public sources, in regard to the extent to which cell- and tissue-specific gene regulatory networks are established. CellNet was originally designed to work with human or mouse microarray expression data for 21 cell or tissue (C/T) types...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28448445/horizontal-directional-drilling-length-detection-technology-while-drilling-based-on-bi-electro-magnetic-sensing
#13
Yudan Wang, Guojun Wen, Han Chen
The drilling length is an important parameter in the process of horizontal directional drilling (HDD) exploration and recovery, but there has been a lack of accurate, automatically obtained statistics regarding this parameter. Herein, a technique for real-time HDD length detection and a management system based on the electromagnetic detection method with a microprocessor and two magnetoresistive sensors employing the software LabVIEW are proposed. The basic principle is to detect the change in the magnetic-field strength near a current coil while the drill stem and drill-stem joint successively pass through the current coil forward or backward...
April 27, 2017: Sensors
https://www.readbyqxmd.com/read/28448434/static-memory-deduplication-for-performance-optimization-in-cloud-computing
#14
Gangyong Jia, Guangjie Han, Hao Wang, Xuan Yang
In a cloud computing environment, the number of virtual machines (VMs) on a single physical server and the number of applications running on each VM are continuously growing. This has led to an enormous increase in the demand of memory capacity and subsequent increase in the energy consumption in the cloud. Lack of enough memory has become a major bottleneck for scalability and performance of virtualization interfaces in cloud computing. To address this problem, memory deduplication techniques which reduce memory demand through page sharing are being adopted...
April 27, 2017: Sensors
https://www.readbyqxmd.com/read/28448426/orexin-receptor-antagonism-in-painful-diabetic-neuropathy-a-phase-2-trial-with-filorexant
#15
W Joseph Herring, Joy Y Ge, Saheeda Jackson, Christopher Assaid, Kathryn M Connor, David Michelson
OBJECTIVES: To evaluate whether orexin receptor antagonism with filorexant provides pain relief in patients with painful diabetic neuropathy (PDN). METHODS: In this double-blind, placebo-controlled, enriched-enrollment, randomized-withdrawal proof-of-concept study, patients with PDN (aged 18-75 y) entered a 2-week, single-blind active run-in period with filorexant 10 mg nightly, before randomization 1:1 to placebo or filorexant in a 2-week, double-blind treatment period...
April 26, 2017: Clinical Journal of Pain
https://www.readbyqxmd.com/read/28448086/effects-of-running-wheel-activity-and-dietary-hmb-and-%C3%AE-alanine-co-supplementation-on-muscle-quality-in-aged-male-rats
#16
D W Russ, C Acksel, K W McCorkle, N K Edens, S M Garvey
OBJECTIVE: Loss of skeletal muscle function is linked to increased risk for loss of health and independence in older adults. Dietary interventions that can enhance aging muscle function, alone or in combination with exercise, may offer an effective way to reduce these risks. The goal of this study was to evaluate the muscular effects of beta-hydroxy-beta-methylbutyrate (HMB) and beta-alanine (β-Ala) co-supplementation in aged Sprague-Dawley rats with voluntary access to running wheels (RW)...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28448032/a-tailored-hplc-purification-protocol-that-yields-high-purity-amyloid-beta-42-and-amyloid-beta-40-peptides-capable-of-oligomer-formation
#17
Christopher J A Warner, Subrata Dutta, Alejandro R Foley, Jevgenij A Raskatov
Amyloidogenic peptides such as the Alzheimer's disease-implicated Amyloid beta (Aβ), can present a significant challenge when trying to obtain high purity material. Here we present a tailored HPLC purification protocol to produce high-purity amyloid beta 42 (Aβ42) and amyloid beta 40 (Aβ40) peptides. We have found that the combination of commercially available hydrophobic poly(styrene/divinylbenzene) stationary phase, polymer laboratory reverse phase - styrenedivinylbenzene (PLRP-S) under high pH conditions, enables the attainment of high purity (>95%) Aβ42 in a single chromatographic run...
March 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448031/transcriptomic-analysis-of-c-elegans-rna-sequencing-data-through-the-tuxedo-suite-on-the-galaxy-project
#18
Francis R G Amrit, Arjumand Ghazi
Next generation sequencing (NGS) technologies have revolutionized the nature of biological investigation. Of these, RNA Sequencing (RNA-Seq) has emerged as a powerful tool for gene-expression analysis and transcriptome mapping. However, handling RNA-Seq datasets requires sophisticated computational expertise and poses inherent challenges for biology researchers. This bottleneck has been mitigated by the open access Galaxy project that allows users without bioinformatics skills to analyze RNA-Seq data, and the Database for Annotation, Visualization, and Integrated Discovery (DAVID), a Gene Ontology (GO) term analysis suite that helps derive biological meaning from large data sets...
April 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447625/randomised-double-blind-placebo-controlled-assessment-of-the-efficacy-and-safety-of-dietary-supplements-in-prehypertension
#19
F Pelliccia, V Pasceri, G Marazzi, A Arrivi, L Cacciotti, G Pannarale, G Speciale, C Greco, C Gaudio
We aimed to evaluate efficacy and tolerability of a protocol including lifestyle modifications and a novel combination of dietary supplements in prehypertension. A prospective, double-blind, randomised, placebo-controlled trial was conducted in 176 subjects (103 men, aged 52±10 years), with blood pressure (BP) of 130-139 mm Hg systolic and/or 85-89 mm Hg diastolic entered. After a single-blind run-in period, participants were randomised to twice daily placebo (n=88) or a commercially available combination pill (n=88)...
April 27, 2017: Journal of Human Hypertension
https://www.readbyqxmd.com/read/28447391/transcriptional-response-of-escherichia-coli-to-ammonia-and-glucose-fluctuations
#20
Joana Danica Simen, Michael Löffler, Günter Jäger, Karin Schäferhoff, Andreas Freund, Jakob Matthes, Jan Müller, Ralf Takors
In large-scale production processes, metabolic control is typically achieved by limited supply of essential nutrients such as glucose or ammonia. With increasing bioreactor dimensions, microbial producers such as Escherichia coli are exposed to changing substrate availabilities due to limited mixing. In turn, cells sense and respond to these dynamic conditions leading to frequent activation of their regulatory programmes. Previously, we characterized short- and long-term strategies of cells to adapt to glucose fluctuations...
April 26, 2017: Microbial Biotechnology
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