Keiichi Tamai, Nobuyuki Tanaka, Takashi Nakano, Eiji Kakazu, Yasuteru Kondo, Jun Inoue, Masaaki Shiina, Koji Fukushima, Tomoaki Hoshino, Kouichi Sano, Yoshiyuki Ueno, Tooru Shimosegawa, Kazuo Sugamura
Exosomes are nanovesicles derived from multivesicular bodies (MVBs) in antigen-presenting cells. The components of the ESCRT (endosomal sorting complex required for transport) pathway are critical for the formation of MVBs, however the relationship between the ESCRT pathway and the secretion of exosomes remains unclear. We here demonstrate that Hrs, an ESCRT-0 protein, is required for fascilitating the secretion of exosomes in dendritic cells (DCs). Ultrastructural analyses showed typical saucer-shaped exosomes in the culture supernatant from both the control and Hrs-depleted DCs...
August 27, 2010: Biochemical and Biophysical Research Communications