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Yu-Dong Ma, Varadharajan Thiyagarajan, May-Jywan Tsai, Sheng-I Lue, Yi-Chen Chia, Song-Kun Shyue, Ching-Feng Weng
Migration of vascular smooth muscle cells (VSMCs) contributes to intimal hyperplasia and other vascular diseases. Caveolin-1 (Cav-1) has been recognized as a proliferative inhibitor of VSMCs and is likely to be an important regulator of VSMC migration. The underlying mechanism of pyrogallol on the VSMC migration is not fully understood. This study attempted to dissect the role of Cav-1 and matrix metalloproteinase (MMP) in VSMC migration and to investigate the effect of pyrogallol on VSMC mobility during carotid artery ligation mice...
October 15, 2016: Environmental Toxicology and Pharmacology
Peter Hammarsten, Tove Dahl Scherdin, Christina Hägglöf, Pernilla Andersson, Pernilla Wikström, Pär Stattin, Lars Egevad, Torvald Granfors, Anders Bergh
In the present study we have investigated whether Caveolin-1 expression in non-malignant and malignant prostate tissue is a potential prognostic marker for outcome in prostate cancer patients managed by watchful waiting. Caveolin-1 was measured in prostate tissues obtained through transurethral resection of the prostate from 395 patients diagnosed with prostate cancer. The majority of the patients (n = 298) were followed by watchful waiting after diagnosis. Tissue microarrays constructed from malignant and non-malignant prostate tissue were stained with an antibody against Caveolin-1...
2016: PloS One
Caroline Moerke, Petra Mueller, Barbara Nebe
The provided data contains the phagocytic interaction of human MG-63 osteoblasts with micro-particles 6 µm in size as well as geometric micro-pillared topography with micro-pillar sizes 5 µm of length, width, height and spacing respectively related to the research article entitled "Attempted caveolae-mediated phagocytosis of surface-fixed micro-pillars by human osteoblasts" in the Biomaterials journal. [1] Micro-particle treatment was used as positive control triggering phagocytosis by the osteoblasts. Caveolin-1 (Cav-1) as major structural component of caveolae [2] plays an important role in the phagocytic process of micro-particles and -pillars...
June 2016: Data in Brief
Moritz Flick, Martin Albrecht, Gezina T M L Oei, Renske Steenstra, Raphaela P Kerindongo, Coert J Zuurbier, Hemal H Patel, Markus W Hollmann, Benedikt Preckel, Nina C Weber
Caveolae, lipid enriched invaginations of the plasma membrane, are epicentres of cellular signal transduction. The structural proteins of caveolae, caveolins, regulate effector pathways in anaesthetic-induced cardioprotection, including the RISK pathway. Helium (He) postconditioning (HePoc) is known to mimic anaesthetic conditioning and to prevent damage from myocardial infarction. We hypothesize that HePoc regulates caveolin-1 and caveolin-3 (Cav-1 and Cav-3) expression in the rat heart and activates the RISK pathway...
October 11, 2016: European Journal of Pharmacology
Kang-Ho Choi, Hyung-Seok Kim, Man-Seok Park, Eun-Bin Lee, Jung-Kil Lee, Joon-Tae Kim, Ja-Hae Kim, Min-Cheol Lee, Hong-Joon Lee, Ki-Hyun Cho
Cerebral edema from the disruption of the blood-brain barrier (BBB) after cerebral ischemia is a major cause of morbidity and mortality as well as a common event in patients with stroke. Caveolins (Cavs) are thought to regulate BBB functions. Here, we report for the first time that Cav-1 overexpression (OE) decreased brain edema from BBB disruption following ischemic insult. Edema volumes and Cav-1 expression levels were measured following photothrombosis and middle cerebral artery occlusion (MCAO). Endothelial cells that were transduced with a Cav-1 lentiviral expression vector were transplanted into rats...
September 29, 2016: Oncotarget
Jing Zhang, Kundong Zhang, Lisheng Zhou, Weidong Wu, Tao Jiang, Jun Cao, Kejian Huang, Zhengjun Qiu, Chen Huang
The aim of the present study was to investigate the expression and functions of Forkhead box protein M1 (FoxM1), Caveolin-1 (Cav-1) and E-cadherin in colorectal cancer (CRC), and to determine the correlations among these proteins in CRC development and progression. The protein expression of FoxM1, Cav-1 and E-cadherin was identified using a human CRC and normal tissue microarray. A standard immunohistochemistry assay was performed employing anti-FoxM1, anti-Cav-1 and anti-E-cadherin antibodies. The clinicopathological significance of FoxM1, Cav-1 and E-cadherin in CRC was determined, and correlations were investigated between FoxM1 and Cav-1, FoxM1 and E-cadherin, Cav-1 and E-cadherin, respectively...
October 2016: Oncology Letters
Aleksandra Piechota-Polanczyk, Marta Zielińska, Damian Piekielny, Jakub Fichna
AIM: This study was undertaken to verify if two-weeks treatment of lipoic acid (LA) influence colon damage and pro-inflammatory cytokine synthesis during DSS-induced acute colitis. Moreover, as LA has anti-oxidative properties, we analyzed its influence on the level of antioxidative enzymes, HO-1 and eNOS, and their regulator- caveolin-1. METHODS: LA was administrated to male C57/BALBc mice at a dose of 25 or 50mg/kg/day (i.p.) for 21days. Acute colitis was induced by administration of 4% DSS (w/v) in drinking water for 5days, followed by 2days of normal drinking water...
September 26, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Rene Baudrand, Nidhi Gupta, Amanda E Garza, Anand Vaidya, Jane A Leopold, Paul N Hopkins, Xavier Jeunemaitre, Claudio Ferri, Jose R Romero, Jonathan Williams, Joseph Loscalzo, Gail K Adler, Gordon H Williams, Luminita H Pojoga
BACKGROUND: Overactivation of the aldosterone and mineralocorticoid receptor (MR) pathway is associated with hyperglycemia and dyslipidemia. Caveolin 1 (cav-1) is involved in glucose/lipid homeostasis and may modulate MR signaling. We investigated the interplay between cav-1 and aldosterone signaling in modulating insulin resistance and dyslipidemia in cav-1-null mice and humans with a prevalent variant in the CAV1 gene. METHODS AND RESULTS: In mouse studies, cav-1 knockout mice exhibited higher levels of homeostatic model assessment of insulin resistance, cholesterol, and resistin and lower ratios of high- to low-density lipoprotein (all P<0...
2016: Journal of the American Heart Association
Flora Gilde, Laure Fourel, Raphael Guillot, Isabelle Pignot-Paintrand, Takaharu Okada, Vincent Fitzpatrick, Thomas Boudou, Corinne Albiges-Rizo, Catherine Picart
: Surface coatings delivering BMP are a promising approach to render biomaterials osteoinductive. In contrast to soluble BMPs which can interact with their receptors at the dorsal side of the cell, BMPs presented as an insoluble cue physically bound to a biomimetic matrix, called here matrix-bound (bBMP-2), are presented to cells by their ventral side. To date, BMP-2 internalization and signaling studies in cell biology have always been performed by adding soluble (sBMP-2) to cells adhered on cell culture plates or glass slides, which will be considered here as a "reference" condition...
September 12, 2016: Acta Biomaterialia
Shalini Gross, Kavi Devraj, Yuxi Feng, Jadranka Macas, Stefan Liebner, Thomas Wieland
Nucleoside diphosphate kinase B (NDPK-B) is an enzyme required for nucleoside triphosphate homeostasis, which has been shown to interact with caveolin-1 (Cav-1). In endothelial cells (ECs), NDPK-B contributes to the regulation of angiogenesis and adherens junction (AJ) integrity. We therefore investigated whether an interaction of NDPK-B with Cav-1 in ECs is required for this regulation and the involvement of VEGF signaling herein. We report that simultaneous depletion of NDPK-B/Cav-1 in HUVECs synergistically impaired sprouting angiogenesis...
September 14, 2016: Journal of Cerebral Blood Flow and Metabolism
Nina Sun, Xiaofang Wei, Jingluan Wang, Zhaozhong Cheng, Weihong Sun
The imbalance of Th17/Treg cells plays an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Caveolin-1 (Cav-1) has been regarded as a potential critical regulatory protein in pathological mechanisms of chronic inflammatory respiratory diseases. Therefore, we investigated whether the loss of Cav-1 is involved in the homeostasis of Th17/Treg cells in COPD. We examined the expressions of plasma Cav-1 and circulating Th17, Treg cells, and the related cytokines in patients with COPD...
September 9, 2016: Inflammation
Yanting Zhang, Xiangzhan Zhu, Tuanjie Huang, Lei Chen, Yanxia Liu, Qinghua Li, Jishi Song, Shanshan Ma, Kun Zhang, Bo Yang, Fangxia Guan
Recently, we reported that β-carotene exhibited anticancer activity against human esophageal squamous cell carcinoma cells in vitro. In the present study, we examined a novel therapeutic strategy by combining β-carotene with 5-fluorouracil (5-FU) in human esophageal cancer in vitro and in vivo, and elucidated the underlying mechanisms. We found that the combination of 5-FU and β-carotene displayed greater growth inhibitory effects than did either compound alone in esophageal squamous cell carcinoma (ESCC) cells...
November 2, 2016: Toxicology Letters
Dan Shang, Tao Peng, Shanmiao Gou, Yiqing Li, Heshui Wu, Chunyou Wang, Zhiyong Yang
High-mobility group box protein 1 (HMGB1), an inflammatory mediator, has been reported to destroy cell-cell junctions, resulting in vascular endothelial hyperpermeability. Here, we report that HMGB1 increases the endothelial transcytosis of albumin. In mouse lung vascular endothelial cells (MLVECs), HMGB1 at a concentration of 500 ng/ml or less did not harm cell-cell junctions but rapidly induced endothelial hyperpermeability to (125)I-albumin. HMGB1 induced an increase in (125)I-albumin and AlexaFluor 488-labeled albumin internalization in endocytosis assays...
2016: Scientific Reports
Yan Y Sanders, Hui Liu, Anne M Scruggs, Steven R Duncan, Steven K Huang, Victor J Thannickal
RATIONALE: Fibrotic disorders are associated with tissue accumulation of fibroblasts. We recently showed that caveolin-1 (Cav-1) gene suppression by the pro-fibrotic cytokine TGF-β1 contributes to fibroblast proliferation and apoptosis-resistance. Cav-1 has been shown to be constitutively suppressed in idiopathic pulmonary fibrosis (IPF), but mechanisms for this suppression are incompletely understood. We hypothesized that epigenetic processes contribute to Cav-1 downregulation in IPF lung fibroblasts, and following fibrogenic stimuli...
August 25, 2016: American Journal of Respiratory Cell and Molecular Biology
Yasuyuki Nakada, Izumi Yamamoto, Shigeru Horita, Akimitsu Kobayashi, Aki Mafune, Haruki Katsumata, Takafumi Yamakawa, Ai Katsuma, Mayuko Kawabe, Yudo Tanno, Ichiro Ohkido, Nobuo Tsuboi, Hiroyasu Yamamoto, Masayoshi Okumi, Hideki Ishida, Takashi Yokoo, Kazunari Tanabe
The low sensitivity of C4d immunoreactivity in peritubular capillaries (PTCs) hinders its use in the diagnosis of chronic active antibody-mediated rejection (CAAMR). C4d-negative CAAMR was defined in the 2013 Banff classification, which included the expression of endothelial-associated transcripts (ENDATs). We previously showed that the ENDAT caveolin-1 (CAV-1) is a distinct feature of CAAMR. In this study, we investigated the prognostic value of CAV-1 immunoreactivity in PTCs in kidney transplant patients...
August 20, 2016: Clinical Transplantation
Yan Bai, Jian Wu, Daxiang Li, Eric E Morgan, Jiang Liu, Xiaochen Zhao, Aaron Walsh, Jagannath Saikumar, Jodi Tinkel, Bina Joe, Rajesh Gupta, Lijun Liu
Binding of ouabain to cardiac Na(+)/K(+)-ATPase initiates cell signaling and causes contractility in cardiomyocytes. It is widely accepted that caveolins, structural proteins of caveolae, have been implicated in signal transduction. It is known that caveolae play a role in Na(+)/K(+)-ATPase functions. Regulation of caveolin-1 in ouabain-mediated cardiac signaling and contractility has never been reported. The aim of this study is to compare ouabain-induced cardiac signaling and contractility in wild-type (WT) and caveolin-1 knockout (cav-1 KO) mice...
October 1, 2016: Physiological Genomics
Duygu Ayaz, Gulden Diniz, Dudu Solakoglu Kahraman, Sevil Sayhan, Melek Uncel, Tugba Karadeniz, Muzaffer Sanci
OBJECTIVES: This retrospective study was designed to evaluate the importance of tissue expressions of caveolin-1 (Cav-1) and AT-rich interactive domain 1 alpha (ARID-1A) which are known as signal regulator and tumor suppressor in differential diagnosis of uterine smooth muscle tumors (SMTs). MATERIALS AND METHODS: Thirty patients recently diagnosed as uterine SMTs at the Tepecik Training and Research Hospital were identified using pathology databases. Immunohistochemical stains for Cav-1 and ARID-1A were performed...
July 2016: Indian Journal of Pathology & Microbiology
Ying Jiang, Maria S Sverdlov, Peter T Toth, Long Shuang Huang, Guangwei Du, Yiyao Liu, Viswanathan Natarajan, Richard D Minshall
Caveolae are the primary route for internalization and transendothelial transport of macromolecules, such as insulin and albumin. Caveolae-mediated endocytosis is activated by Src-dependent caveolin-1 (Cav-1) phosphorylation and subsequent recruitment of dynamin-2 and filamin A (FilA), which facilitate vesicle fission and trafficking, respectively. Here, we tested the role of RalA and phospholipase D (PLD) signaling in the regulation of caveolae-mediated endocytosis and trafficking. The addition of albumin to human lung microvascular endothelial cells induced the activation of RalA within minutes, and siRNA-mediated down-regulation of RalA abolished fluorescent BSA uptake...
September 23, 2016: Journal of Biological Chemistry
Heqin Zhan, Feng Huang, Wenzhuo Ma, Zhenghang Zhao, Haifang Zhang, Chong Zhang
Idiopathic pulmonary fibrosis (IPF) is a progressive disease with poor prognosis and high mortality rate. Panax Notoginseng Saponins (PNS), extracted from Panax Notoginseng as a traditional Asian medicine, displayed a significant anti-fibrosis effect in liver and lung. However, whether Ginsenoside Rg1 (Rg1), an important and active ingredient of PNS, exerts anti-fibrotic activity on IPF still remain unclear. In this study, we investigated the effect of Rg1 on bleomycin-induced pulmonary fibrosis in rats. Bleomycin (5 mg/kg body weight) was intratracheally administrated to male rats...
2016: Biological & Pharmaceutical Bulletin
Valentina Vellecco, Emma Mitidieri, Antonella Gargiulo, Vincenzo Brancaleone, Danilo Matassa, Thomas Klein, Franca Esposito, Giuseppe Cirino, Mariarosaria Bucci
AIM: To test the effect of linagliptin in non-obese diabetic (NOD) mice, a murine model of type 1 diabetes, to unveil a possible direct cardiovascular action of dipeptidyl peptidase 4 (DPP-4) inhibitors beyond glycaemia control. METHODS: NOD mice were grouped according to glycosuria levels as NODI: none; NODII: high; NODIII: severe. Linagliptin treatment was initiated once they reached NODII levels. Vascular reactivity was assessed ex vivo on aorta harvested from mice upon reaching NODIII level...
July 27, 2016: Diabetes, Obesity & Metabolism
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