Read by QxMD icon Read


Huiyu Liu, Yan Gao, Bo Zhai, Hao Jiang, Yu Ding, Lianjiang Zhang, Changhong Li, Qiong Deng, Xianfeng Yu, Jiabao Zhang
AIMS: This study aims to clarify the effects of polyadenylation status on M-phase promoting factors (MPFs) during in vitro porcine oocyte maturation. METHODS: In this study, porcine follicular oocytes from large follicles (> 5 millimeter (mm)) and small follicles (< 3 mm) were examined at different follicular developmental stages. The polyadenylation of maternal mRNAs was inhibited by the addition of 3'-deoxyadenosine (3'-da) during the germinal vesicle (GV)(0 h), GV breakdown (GVBD)(18 h), metaphase I (MI)(28 h), and metaphase II (MII) (44 h) stages...
October 17, 2016: Cellular Physiology and Biochemistry
Tatsuya Shiraki, Osamu Iida, Mitsuyoshi Takahara, Yoshimitsu Soga, Shinsuke Mii, Jin Okazaki, Sosei Kuma, Terutoshi Yamaoka, Daisuke Kamoi, Yoshiaki Shintani, Toshinobu Ishikawa, Ikuro Kitano, Masaaki Uematsu
AIM: The treatment strategy for hemodialysis (HD) patients with critical limb ischemia (CLI) has been clinically debatable. Here we compared clinical outcomes after bypass surgery (BSX) and after endovascular therapy (EVT) using propensity score matching. METHODS: A retrospective multicenter database of 246 (68 BSX and 178 EVT) consecutive HD patients with CLI (79% with tissue loss) who underwent infrainguinal revascularization from 2007 to 2009 was used to compare clinical outcomes, including overall survival (OS), major amputation (MA), major adverse limb event (MALE: repeat EVT, surgical reconstruction, or MA), and MALE-free survival after BSX vs...
October 13, 2016: Journal of Atherosclerosis and Thrombosis
Yukou Sugano, Manami Yazawa, Sachio Takino, Sueo Niimura, Hideaki Yamashiro
The objective of this study was to classify spindle and first polar body (PB1) chromosome images in ovulated mouse oocytes over time to predict the developmental competence of metaphase II (MII) oocytes. Oocytes were collected at 12, 15, 20, and 25 h after human chorionic gonadotropin (hCG) injection, and stained for spindle tubulin, chromosomes, and PB1 chromosomes. MII spindle morphology was classified as tapered type or barrel type and PB1 chromosomes were categorized as aggregated, separated, dot, or collapsed...
October 13, 2016: Zygote: the Biology of Gametes and Early Embryos
Ivan Pavić, Irena Babić, Jasna Čepin Bogović, Iva Hojsak
OBJECTIVE: The aim of this study was to investigate the diagnostic usefulness of combined multichannel intraluminal impedance-pH (MII-pH) monitoring in children with suspected laryngopharyngeal reflux (LPR). DESIGN, SETTING AND PARTICIPANTS: A prospective study including children in whom, due to LPR suggestive symptoms, MII-pH monitoring was performed at tertiary medical center from February 2012 to July 2015. INTERVENTIONS: All included children underwent same diagnostic protocol which included examination by single pulmonologist, ENT specialist and underwent 24-h MII-pH monitoring...
October 11, 2016: Clinical Otolaryngology
Yue Zhang, Wei Li, Yongshun Ma, Dian Wang, Xiaoxue Zhao, Changjun Zeng, Ming Zhang, Xianyin Zeng, Qinggang Meng, Guangbin Zhou
The study was aimed to investigate the effect of melatonin on the development potential of mouse MII oocytes after cryopreservation. Mouse MII oocytes were subjected first to vitrification/warming and 2 h of in vitro culture (phase 1), then to parthenogenetic activation (PA) followed by in vitro culture of parthenogenetic embryos (phase 2). Different concentrations of melatonin (0, 10(-9), 10(-6) mol/L) were added to the medium during either phase 1, phase 2 or both phases. The fresh oocytes were used as control...
October 7, 2016: Cryobiology
Mayuree Kanlayavattanakul, Nattaya Lourith, Puxvadee Chaikul
ETHNOPHARMACOLOGICAL RELEVANCE: While rice is one of the most important global staple food sources its extracts have found many uses as the bases of herbal remedies. Rice extracts contain high levels of phenolic compounds which are known to be bioactive, some of which show cutaneous benefits and activity towards skin disorders. This study highlights an assessment of the cellular activity and clinical efficacy of rice panicle extract, providing necessary information relevant to the development of new cosmetic products...
October 6, 2016: Journal of Ethnopharmacology
Liyuan Fu, Mingxiang Zhang, Kristen Mastrantoni, Mark Perfetto, Shuo Wei, Jianbo Yao
LIM homeobox 8 (Lhx8) is a germ cell-specific transcription factor essential for the development of oocytes during early oogenesis. In mice, Lhx8 deficiency causes postnatal oocyte loss and affects the expression of many oocyte-specific genes. The aims of this study were to characterize the bovine Lhx8 gene, determine its mRNA expression during oocyte development and early embryogenesis, and evaluate its interactions with other oocyte-specific transcription factors. The bovine Lhx8 gene encodes a protein of 377 amino acids...
2016: PloS One
Yuki Sato, Akiko Mii, Yoko Hamazaki, Harumi Fujita, Hirosuke Nakata, Kyoko Masuda, Shingo Nishiyama, Shinsuke Shibuya, Hironori Haga, Osamu Ogawa, Akira Shimizu, Shuh Narumiya, Tsuneyasu Kaisho, Makoto Arita, Masashi Yanagisawa, Masayuki Miyasaka, Kumar Sharma, Nagahiro Minato, Hiroshi Kawamoto, Motoko Yanagita
Acute kidney injury (AKI) is a common clinical condition defined as a rapid decline in kidney function. AKI is a global health burden, estimated to cause 2 million deaths annually worldwide. Unlike AKI in the young, which is reversible, AKI in the elderly often leads to end-stage renal disease, and the mechanism that prevents kidney repair in the elderly is unclear. Here we demonstrate that aged but not young mice developed multiple tertiary lymphoid tissues (TLTs) in the kidney after AKI. TLT size was associated with impaired renal function and increased expression of proinflammatory cytokines and homeostatic chemokines, indicating a possible contribution of TLTs to sustained inflammation after injury...
July 21, 2016: JCI Insight
Y Lee, H Lee, B Park, F Elahi, J Lee, S T Lee, C K Park, S H Hyun, E Lee
The purpose of this study was to examine the effects of alpha-linolenic acid (ALA) treatment during in vitro maturation (IVM) on nuclear maturation, intraoocyte glutathione (GSH) content, meiotic progression, and developmental competence after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. Medium-199 containing 10% (vol/vol) porcine follicular fluid (PFF; PPF control) or 0.4% (wt/vol) fatty acid-free BSA (BSA control) was used for IVM. The proportion of oocytes reaching the metaphase II (MII) stage was not influenced by ALA treatment at various concentrations (50, 100, and 200 μ)...
August 2016: Journal of Animal Science
M M J Singendonk, M A Benninga, M P van Wijk
Recently, multichannel intraluminal impedance (MII) monitoring was added to the repertoire of tests to evaluate the (patho)physiology of gastroesophageal reflux (GER) in children. Its advantage above the sole monitoring of the esophageal pH lies in the ability of the detection of both acid and nonacid GER and to discern between liquid and gas GER. Currently, combined 24 h pH-MII monitoring is recommended for evaluation of gastro-esophageal reflux disease (GERD) and its relation to symptoms in infants and children, despite the lack of reference values in these age groups...
October 2016: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
Davide Barloscio, Elisa Cerri, Luciano Domenici, Renato Longhi, Clelia Dallanoce, Milena Moretti, Antonietta Vilella, Michele Zoli, Cecilia Gotti, Nicola Origlia
Although α6-contaning (α6*) nicotinic acetylcholine receptors (nAChRs) are densely expressed in the visual system, their role is not well known. We have characterized a family of toxins that are antagonists for α6β2* receptors and used one of these [RDP-MII(E11R)] to localize α6* nAChRs and investigate their impact on retinal function in adult Long-Evans rats. The α6*nAChRs in retinal tissue were localized using either a fluorescently tagged [RDP-MII(E11R)] or anti-α6-specific antibodies and found to be predominantly at the level of the ganglion cell layer...
September 28, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Simon Nennstiel, Mack Andrea, Mohamed Abdelhafez, Bernhard Haller, Roland M Schmid, Monther Bajbouj, Valentin Becker
BACKGROUND AND STUDY AIM: Optimal therapy concepts in patients with laryngo-pharyngeal reflux (LPR) are still under discussion. Aim of this study was to evaluate long term symptom relief according to results in combined pH/multichannel impedance (MII) monitoring to predict therapy response and symptom relief during long term follow-up. PATIENTS AND METHODS: In patients with predominant LPR symptoms, pH/MII monitoring and subsequent proton pump inhibitor (PPI) therapy were evaluated retrospectively after a minimum follow-up period of 36months...
September 2016: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
Srinivasan Natarajan, Jagannatha Gopalakrishnan, Subramani Tamilarasan, M L P Reddy
We describe the synthesis, crystal structures and optical absorption spectra/colours of 3d-transition metal substituted α-LiZnBO3 derivatives: α-LiZn1-xMIIxBO3 [MII = CoII (0 < x < 0.50), NiII (0 < x ≤ 0.05) and CuII (0 < x ≤ 0.10)] and α-Li1+xZn1-2xMIIIxBO3 [MIII = MnIII (0 < x ≤ 0.10) and FeIII (0 < x ≤ 0.25)]. The crystal structure of the host α-LiZnBO3, which is both disordered and distorted with respect to Li and Zn occupancies and coordination geometries, is largely retained in the derivatives, giving rise to unique colours [blue for CoII, magenta for NiII and violet for CuII], that could be of significance for the development of new, inexpensive and environmentally-benevolent pigment materials, especially for the blue colour...
September 23, 2016: Chemistry, An Asian Journal
B Merlo, E Iacono, D Bucci, M Spinaci, G Galeati, G Mari
In vitro embryo production in the horse is still not as efficient as in other species. Oxidative stress negatively affects oocyte and embryo culture. To attenuate/minimize the oxidative stress, antioxidants such as low-molecular thiol compounds can be added to culture media. Beta-mercaptoethanol (BME) has been shown to improve maturation and embryo development in different species. The aim of this study was to investigate whether the addition to maturation medium of BME at common (0.1 mM) and high (0.7 mM) concentration could improve oocyte maturation also in the horse...
September 21, 2016: Reproduction in Domestic Animals, Zuchthygiene
E M Sadeesh, P Sikka, A K Balhara, S Balhara
The study examined the effects of different environmental stress on developmental competence and the relative abundance (RA) of various gene transcripts in oocytes and embryos of buffalo. Oocytes collected during cold period (CP) and hot period (HP) were matured, fertilized and cultured in vitro to blastocyst hatching stage. The mRNA expression patterns of genes implicated in developmental competence (OCT-4, IGF-2R and GDF-9), heat shock (HSP-70.1), oxidative stress (MnSOD), metabolism (GLUT-1), pro-apoptosis (BAX) and anti-apoptosis (BCL-2) were evaluated in immature and matured oocytes as well as in pre-implantation stage embryos...
September 20, 2016: Cytotechnology
Yoshie A Fujioka, Asuka Onuma, Wataru Fujii, Koji Sugiura, Kunihiko Naito
Cyclin B (CCNB) accumulation is essential for regulating maturation/M-phase promoting factor activity during vertebrate oocyte maturation. Anaphase-promoting-complex/cyclosome (APC/C) degrades CCNB, allowing the cell cycle to progress; this complex is inhibited by Early mitotic inhibitors 1 and 2 (EMI1 and EMI2). The involvement of both EMI proteins in meiotic maturation has been reported in Xenopus and mouse oocytes, although a recent study described a marked difference in their respective function during meiotic resumption...
September 20, 2016: Molecular Reproduction and Development
Nicola de Bortoli, Leonardo Frazzoni, Edoardo V Savarino, Marzio Frazzoni, Irene Martinucci, Aleksandra Jania, Salvatore Tolone, Michele Scagliarini, Massimo Bellini, Elisa Marabotto, Manuele Furnari, Giorgia Bodini, Salvatore Russo, Lorenzo Bertani, Veronica Natali, Lorenzo Fuccio, Vincenzo Savarino, Corrado Blandizzi, Santino Marchi
OBJECTIVES: We aimed to evaluate the prevalence of irritable bowel syndrome (IBS) in patients with typical reflux symptoms as distinguished into gastroesophageal reflux disease (GERD), hypersensitive esophagus (HE), and functional heartburn (FH) by means of endoscopy and multichannel intraluminal impedance (MII)-pH monitoring. The secondary aim was to detect pathophysiological and clinical differences between different sub-groups of patients with heartburn. METHODS: Patients underwent a structured interview based on questionnaires for GERD, IBS, anxiety, and depression...
September 20, 2016: American Journal of Gastroenterology
Y Niu, J Dai, C Wu, Y Chen, S Zhang, D Zhang
Apoptosis is one of the main drivers of the decline in developmental potential of porcine oocytes after vitrification. However, which apoptotic pathways are engaged after vitrification remains poorly understood. To distinguish among the possible apoptotic pathways induced by vitrification of MII stage porcine oocytes, this study detected activity and expression levels of several key proteins and genes in both the death receptor and mitochondrial pathways using in situ fluorescence staining and real-time PCR (RT-PCR) following the addition of specific inhibitors of either the death receptor or the mitochondrial apoptotic pathway (Z-IETD-FMK or Z-LEHD-FMK, respectively) into the incubation solution...
September 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
Chunlei Song, Jing Yao, Chunwei Cao, Xiaojuan Liang, Jiaojiao Huang, Ziqiang Han, Yang Zhang, Guosong Qin, Cong Tao, Chengbo Li, Haoran Yang, Jianguo Zhao, Kui Li, Yanfang Wang
To elucidate the key miRNAs and the signalling pathways that are involved in porcine oocyte maturation, we performed a deep sequencing analysis of the miRNAs of pig germinal vesicle (GV) oocytes and metaphase II (MII) oocytes. Seven differentially expressed (DE) miRNAs were identified and the expression levels of miR-21 and miR-27b-3p were further confirmed by QPCR analysis. The target genes of 7 DE miRNAs were predicted and subjected to pathway analysis. Interestingly, fatty acid metabolism and fatty acid biosynthesis were the top two significantly enriched molecular functions during oocyte maturation...
October 14, 2016: Biochemical and Biophysical Research Communications
Serhat Bor, Askin Erdogan, Berna Bayrakci, Esra Yildirim, Rukiye Vardar
Obesity increases the risk of gastroesophageal reflux disease (GERD). The majority of the reflux attacks occur postprandially. The influence of the speed of food intake on gastroesophageal reflux events is unclear in obese patients. To determine the influence of the speed of food intake on intraesophageal reflux events in obese patients with and without GERD. A total of 26 obese female patients were recruited. The patients underwent esophageal manometry to evaluate the upper limit of the lower esophageal sphincter and subsequently placement of a Multichannel intraluminal impedance-pH (MII-pH) catheter...
September 15, 2016: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"