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https://www.readbyqxmd.com/read/28719824/supplementation-of-l-carnitine-during-in%C3%A2-vitro-maturation-improves-embryo-development-from-less-competent-bovine-oocytes
#1
Drahomira Knitlova, Pavlina Hulinska, Michal Jeseta, Katerina Hanzalova, Bartosz Kempisty, Marie Machatkova
The present study was designed to define the impact of l-carnitine, supplemented during maturation, on bovine oocytes with different meiotic competence in terms of their IVF outcomes. Meiotically more competent (MMC) and less competent (MLC) oocytes were obtained separately from differently sized follicles at selected phases of folliculogenesis. The oocytes were matured with or without l-carnitine, fertilized and cultured to the blastocyst stage. The oocytes were examined for nuclear maturation, mitochondrial cluster formation, lipid consumption, fertilization and embryo development...
July 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28717067/barthel-index-and-outcome-of-open-bypass-for-critical-limb-ischemia
#2
Shinsuke Mii, Atsushi Guntani, Eisuke Kawakubo, Hirofumi Shimazoe
BACKGROUND: Daily activity should be an important factor to consider when deciding on a treatment strategy for critical limb ischemia (CLI), and we hypothesized that there was a close relationship between activities of daily living (ADL) and prognosis. The aim of this study was to investigate the association between ADL and outcomes of open bypass for CLI.Methods and Results:A total of 226 patients undergoing infrainguinal bypass for CLI between January 2005 and December 2015 were reviewed...
July 15, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28706605/embryonic-stem-cell-conditioned-medium-supports-in-vitro-maturation-of-mouse-oocytes
#3
Saber Miraki, Aram Mokarizadeh, Omid Banafshi, Vahideh Assadollahi, Mahdad Abdi, Daem Roshani, Fardin Fathi
BACKGROUND: This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). METHODS: Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized in vitro...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28702007/identification-bioactivity-and-productivity-of-actinomycins-from-the-marine-derived-streptomyces-heliomycini
#4
Dongyang Wang, Cong Wang, Pengyan Gui, Haishan Liu, Sameh M H Khalaf, Elsayed A Elsayed, Mohammed A M Wadaan, Wael N Hozzein, Weiming Zhu
In the process of profiling the secondary metabolites of actinobacteria isolated from the Saudi coastal habitats for production of antibiotics and anti-cancer drugs, the cultures of strain WH1 that was identified as Streptomyces heliomycini exhibited strong antibacterial activity against Staphylococcus aureus. By means of MS and NMR techniques, the active compounds were characterized as actinomycins X0β, X2, and D, respectively. The research on the productivity of this strain for actinomycins revealed that the highest production of actinomycins X0β, X2, and D was reached in the medium MII within 5% salinity and pH 8...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28695963/differential-regulation-of-cumulus-cell-transcription-during-oocyte-maturation-in-vivo-and-in-vitro
#5
Giovanni Coticchio, Libby Ophir, Yuval Yung, Micha Baum, Mariabeatrice Dal Canto, Mario Mignini-Renzini, Fausta Brambillasca, Rubens Fadini, Ariel Hourvitz
Differences in cumulus cell gene expression after oocyte maturation in vitro (IVM) or in vivo have been described in previous studies. However, the possible impact of follicle stage on gene expression deregulation during human oocyte IVM remains unknown. Expression of selected genes of interest was compared in cumulus cell of three classes of human cumulus cell-oocyte complexes (COCs): a) COCs derived from human chorionic gonadotropin (hCG)-triggered IVM cycles, collected at the germinal vesicle (GV) stage from mid-sized follicles (4-12 mm) and matured in vitro (IVM-GV); b) COCs derived from hCG-triggered IVM cycles, collected from mid-sized follicles (4-12 mm) and matured in vivo (IVM-MII); c) COCs derived from controlled ovarian stimulation in vitro fertilization (IVF) cycles, collected from large/preovulatory follicles and matured in vivo (IVF-MII)...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28676721/nonlinear-climatic-sensitivity-to-greenhouse-gases-over-past-4-glacial-interglacial-cycles
#6
Li Lo, Sheng-Pu Chang, Kuo-Yen Wei, Shih-Yu Lee, Tsong-Hua Ou, Yi-Chi Chen, Chih-Kai Chuang, Horng-Sheng Mii, George S Burr, Min-Te Chen, Ying-Hung Tung, Meng-Chieh Tsai, David A Hodell, Chuan-Chou Shen
The paleoclimatic sensitivity to atmospheric greenhouse gases (GHGs) has recently been suggested to be nonlinear, however a GHG threshold value associated with deglaciation remains uncertain. Here, we combine a new sea surface temperature record spanning the last 360,000 years from the southern Western Pacific Warm Pool with records from five previous studies in the equatorial Pacific to document the nonlinear relationship between climatic sensitivity and GHG levels over the past four glacial/interglacial cycles...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28670426/comparative-stepwise-pattern-of-reactive-oxygen-species-production-during-in-vitro-development-of-fertilized-and-nuclear-transferred-goat-embryos
#7
Mehdi Hajian, Sayed Morteza Hosseini, Somayyeh Ostadhosseini, Mohammad Hossein Nasr-Esfahani
BACKGROUND: A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somatic cell nuclear transfer (SCNT). Furthermore, the pattern of ROS production of SCNT embryos were compared with zona free embryos derived from in vitro fertilization (IVF)...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28670425/vitrification-of-human-germinal-vesicle-oocytes-before-or-after-in-vitro-maturation
#8
Evangelia Kasapi, Byron Asimakopoulos, Katerina Chatzimeletiou, Stamatios Petousis, Yannis Panagiotidis, Nikos Prapas, Nikos Nikolettos
BACKGROUND: The use of immature oocytes derived from stimulated cycles could be of great importance, particularly for urgent fertility preservation cases. The current study aimed to determine whether in vitro maturation (IVM) was more successful before or after vitrification of these oocytes. MATERIALS AND METHODS: This prospective study was performed in a private in vitro fertilization (IVF) center. We collected 318 germinal vesicle (GV) oocytes from 104 stimulated oocyte donation cycles...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28669375/morphogenesis-related-gene-expression-profile-in-porcine-oocytes-before-and-after-in-vitro-maturation
#9
Joanna Budna, Adrian Chachuła, Dominika Kaźmierczak, Marta Rybska, Sylwia Ciesiółka, Artur Bryja, Wiesława Kranc, Sylwia Borys, Agnieszka Żok, Dorota Bukowska, Paweł Antosik, Małgorzata Bruska, Klaus P Brüssow, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
Mammalian oocyte maturation is achieved when oocytes reach metaphase II (MII) stage, and accumulate mRNA and proteins in the cytoplasm following fertilization. It has been shown that oocytes investigated before and after in vitro maturation (IVM) differ significantly in transcriptomic and proteomic profiles. Additionally, folliculogenesis and oogenesis is accompanied by morphogenetic changes, which significantly influence further zygote formation and embryo growth. This study aimed to determine new transcriptomic markers of porcine oocyte morphogenesis that are associated with cell maturation competence...
July 3, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28666811/%C3%AE-6%C3%AE-2-subunit-containing-nicotinic-acetylcholine-receptors-exert-opposing-actions-on-rapid-dopamine-signaling-in-the-nucleus-accumbens-of-rats-with-high-versus-low-response-to-novelty
#10
Cody A Siciliano, J Michael McIntosh, Sara R Jones, Mark J Ferris
Determining neurobiological factors that contribute to individual variance in drug addiction vulnerability allows for identification of at-risk populations, use of preventative measures and personalized medicine in the treatment of substance use disorders. Rodents that exhibit high locomotor activity when exploring an inescapable novel environment (high-responder; HR) are more susceptible to the reinforcing effects of many abused compounds, including nicotine, as compared to animals that exhibit low locomotor activity (low-responder; LR)...
June 27, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28662503/the-effects-of-switching-to-vonoprazan-a-novel-potassium-competitive-acid-blocker-on-gastric-acidity-and-reflux-patterns-in-patients-with-erosive-esophagitis-refractory-to-proton-pump-inhibitors
#11
Hiroshi Yamashita, Atsushi Kanamori, Chise Kano, Hiroki Hashimura, Kei Matsumoto, Masahiro Tsujimae, Tetsuya Yoshizaki, Kenji Momose, Daisuke Obata, Takaaki Eguchi, Mikio Fujita, Akihiko Okada
BACKGROUND/AIM: The effects of vonoprazan and proton pump inhibitors (PPIs) in patients with reflux esophagitis (RE) have not yet been compared using multichannel intraluminal impedance-pH (MII-pH). METHODS: A total of 8 patients with persistent gastric mucosal injury, despite completing an 8-week standard PPI therapy, were enrolled in the study. While they were on standard PPI therapy, the baseline values of reflux parameters, holding time ratio (HTR) of gastric pH >4, and esophageal pH <4 were obtained by using 24 h MII-pH monitoring...
June 30, 2017: Digestion
https://www.readbyqxmd.com/read/28659670/is-the-routine-use-of-impedance-analysis-for-the-diagnosis-of-gastro-esophageal-reflux-disease-more-expensive-than-conventional-ph-monitoring-cost-analysis-of-two-procedures
#12
Nurkan Törer, Özgür Aytaç
Conventional 24-h pH monitoring is the gold standard for the diagnosis of non-erosive, non-acidic gastro-esophageal reflux disease. Multichannel Intraluminal Impedance (MII) analysis markedly improves diagnostic accuracy of non-erosive, non-acidic gastro-esophageal reflux disease. However, MII catheters are more expensive than standard catheters. This study aimed to determine the rates of acid or non-acid reflux and by making a retrospective projection, to predict the costs of two algorithms. We retrospectively reviewed the medical data of 102 patients who presented to our hospital with suspected non-erosive GERD and underwent 24 h impedance/pH-monitoring...
June 2017: Indian Journal of Surgery
https://www.readbyqxmd.com/read/28652254/oviductal-microvesicles-and-their-effect-on-in-vitro-maturation-of-canine-oocytes
#13
Anna Lange-Consiglio, Claudia Perrini, Giulia Albini, Silvia Modina, Valentina Lodde, Eleonora Orsini, Paola Esposti, Fausto Cremonesi
The effect of conditioned medium (CM) or microvesicles (MVs), secreted by multicellular spheroids of oviductal cells, and the involvement of some microRNAs (miRNAs) were investigated in canine oocyte maturation. To generate CM, spheroids were cultured for 3 days. MVs were obtained by ultracentrifugation of CM at 100,000 g and measured for size and concentration by NanoSight instrument. Cumulus-oocyte complexes (COCs) were matured at 38.5°C with 5% CO2 and 5% of O2 in synthetic oviductal fluid (SOF) in biphasic systems: for 24 h, with 5...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28651359/altered-mirna-and-pirna-profiles-in-cumulus-cells-from-patients-with-diminished-ovarian-reserve
#14
Dawei Chen, Zhiguo Zhang, Beili Chen, Dongmei Ji, Yan Hao, Ping Zhou, Zhaolian Wei, Yunxia Cao
Diminished ovarian reserve (DOR) is defined as decreased number or quality of follicles and oocytes in a woman at childbearing age. It is estimated that up to 10% of women in the general population may suffer from DOR. The present study aims to comprehensively characterize miRNA and piRNA expression profiles in cumulus cells of DOR patients. Cumulus cells were collected from 20 women of similar chronological age who received assisted reproductive technology treatment, 10 with diminished ovarian reserve (DOR), 10 with normal ovarian reserve (NOR)...
June 23, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28647785/fertility-preservation-for-trans-men-frozen-thawed-in-vitro-matured-oocytes-collected-at-the-time-of-ovarian-tissue-processing-exhibit-normal-meiotic%C3%A2-spindles
#15
S Lierman, K Tilleman, K Braeckmans, K Peynshaert, S Weyers, G T'Sjoen, P De Sutter
PURPOSES: At the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary...
June 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28647355/random-start-ovarian-stimulation-in-women-desiring-elective-cryopreservation-of-oocytes
#16
Nigel Pereira, Anna Voskuilen-Gonzalez, Kolbe Hancock, Jovana P Lekovich, Glenn L Schattman, Zev Rosenwaks
The current study investigates the utility of random-start ovarian stimulation in women desiring elective oocyte cryopreservation. Women in the study cohort underwent random-start ovarian stimulation, and were subdivided based on the phase of the menstrual cycle that ovarian stimulation began, i.e. early follicular, late follicular or luteal phase. Women undergoing conventional cycle day (CD) 2/3 ovarian stimulation start were controls. A total of 1302 women were included - 859 (66.0%) conventional CD 2/3, 342 (26...
June 12, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28637085/successful-delivery-derived-from-cryopreserved-rare-human-spermatozoa-with-novel-cryopiece
#17
J Sun, W Chen, L Zhou, J Hu, Z Li, Z Zhang, Y Wu
Herein, we report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with cryopiece cryopreserved rare human spermatozoa from severe male factor infertility patients. We established a novel cryopiece system on the basis of previous studies. In this study, 126 spermatozoa from four patients with non-obstructiveazoospermia (NOA) or severe oligozoospermia were stored in cryopiece and then thawed on the day of the oocyte retrieval, 88 (83%) spermatozoa were recovered with a 47.5% (38 of 80) motile rate...
June 21, 2017: Andrology
https://www.readbyqxmd.com/read/28631074/no-difference-in-mitochondrial-distribution-is-observed-in-human-oocytes-after-cryopreservation
#18
Martin Stimpfel, Eda Vrtacnik-Bokal, Irma Virant-Klun
PURPOSE: The primary aim of this study was to determine if any difference in mitochondrial distribution can be observed between fresh and cryopreserved (slow-frozen/thawed and vitrified/warmed) oocytes when oocytes are stained with Mitotracker Red CMXRos and observed under a conventional fluorescent microscope. Additionally, the influence of cryopreservation procedure on the viable rates of oocytes at different maturation stages was evaluated. METHODS: The germinal vesicle (GV) and MII oocytes were cryopreserved with slow-freezing and vitrification...
August 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28621399/-mii-ncs-2-nia-2-oh2-2-preparation-crystal-structure-and-thermal-properties-mii-mn-fe-nia-nicotinamide
#19
Marta Počkaj, Nives Kitanovski, Boris Čeh, Romana Cerc-Korošec
Two novel isostructural coordination compounds of manganese(II) (1) and iron(II) (2) with common formulae [MII(NCS)2(nia)2(OH2)2] have been prepared from water solution of appropriate metal salt, nicotinamide and KSCN. Their crystal structures were determined by means of X-ray diffraction on single crystals. The mononuclear title compounds crystallize in a triclinic P-1 space group with six monodentate octahedrally arranged ligands around the metal centre. The coordination molecules are self-assembled with an extended network of hydrogen bonds into a three-dimensional structure...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28620352/efficacy-of-follicle-stimulating-hormone-fsh-alone-fsh%C3%A2-%C3%A2-luteinizing-hormone-human-menopausal-gonadotropin-or-fsh%C3%A2-%C3%A2-human-chorionic-gonadotropin-on-assisted-reproductive-technology-outcomes-in-the-personalized-medicine-era-a-meta-analysis
#20
Daniele Santi, Livio Casarini, Carlo Alviggi, Manuela Simoni
SETTING: Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) act on the same receptor, activating different signal transduction pathways. The role of LH or hCG addition to follicle-stimulating hormone (FSH) as well as menopausal gonadotropins (human menopausal gonadotropin; hMG) in controlled ovarian stimulation (COS) is debated. OBJECTIVE: To compare FSH + LH, or FSH + hCG or hMG vs. FSH alone on COS outcomes. DESIGN: A meta-analysis according to PRISMA statement and Cochrane Collaboration was performed, including prospective, controlled clinical trials published until July 2016, enrolling women treated with FSH alone or combined with other gonadotropins...
2017: Frontiers in Endocrinology
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