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https://www.readbyqxmd.com/read/28822084/giant-biatrial-myxoma-with-two-different-gross-findings
#1
Taiyo Kuroda, Yukifusa Yokoyama, Satoshi Yuhara, Hideyuki Okawa, Hiroki Hasegawa, Jun Yokote, Shuji Tamaki, Shinji Mii
We experienced a giant biatrial myxoma concomitant with hepatocellular carcinoma. Most of myxomas originate from the left atrium, and biatrial myxomas are extremely rare. Excision of the giant cardiac tumor was performed to avoid risks of life-threatening complications. The resected mass was grossly composed of two parts with the border of interatrial septum and with the shape of peanut shell. Although microscopic examinations revealed enlarged vessels, hemorrhages and hemosiderosis in the left part and high cellularity with chronic inflammation in the right part, spindle-shaped cells in a loose myxoid stroma were observed in both parts of the tumor, consistent with the diagnosis of myxoma...
August 18, 2017: General Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28822010/vitrification-of-human-immature-oocytes-before-and-after-in-vitro-maturation-a-review
#2
REVIEW
Mohammad Ali Khalili, Abbas Shahedi, Sareh Ashourzadeh, Stefania Annarita Nottola, Guido Macchiarelli, Maria Grazia Palmerini
The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing...
August 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28819014/cyclin-a2-modulates-kinetochore-microtubule-attachment-in-meiosis-ii
#3
Qing-Hua Zhang, Wai Shan Yuen, Deepak Adhikari, Jennifer A Flegg, Greg FitzHarris, Marco Conti, Piotr Sicinski, Ibtissem Nabti, Petros Marangos, John Carroll
Cyclin A2 is a crucial mitotic Cdk regulatory partner that coordinates entry into mitosis and is then destroyed in prometaphase within minutes of nuclear envelope breakdown. The role of cyclin A2 in female meiosis and its dynamics during the transition from meiosis I (MI) to meiosis II (MII) remain unclear. We found that cyclin A2 decreases in prometaphase I but recovers after the first meiotic division and persists, uniquely for metaphase, in MII-arrested oocytes. Conditional deletion of cyclin A2 from mouse oocytes has no discernible effect on MI but leads to disrupted MII spindles and increased merotelic attachments...
August 17, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28815026/the-diagnostic-value-of-24-hour-ambulatory-intraesophageal-ph-impedance-in-patients-with-laryngopharyngeal-reflux-symptoms-comparable-with-typical-symptoms
#4
Yusuf S Sakin, Rukiye Vardar, Baha Sezgin, Zeynep Erdogan Cetin, Yasemin Alev, Esra Yildirim, Tayfun Kirazli, Serhat Bor
BACKGROUND: The diagnosis of laryngopharyngeal reflux is currently based on a combination of the patient history of multichannel intraluminal impedance and ambulatory pH (MII-pH); however, none of these findings alone is specific for the diagnosis of laryngopharyngeal reflux. We aimed to compare the baseline characteristics and esophageal baseline impedance values between patients with and without laryngopharyngeal reflux symptoms. METHODS: We retrospectively analyzed data from two groups of patients with laryngopharyngeal reflux according to their reflux finding score (RFS) as scored by ENTs...
August 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28801962/the-charge-transfer-approach-to-heavier-main-group-element-radicals-in-transition-metal-complexes
#5
Gengwen Tan, Jing Li, Li Zhang, Chao Chen, Yue Zhao, Xinping Wang, You Song, Yi-Quan Zhang, Matthias Driess
The phosphorus radical coordinated CoII and FeII complexes 1Co and 1Fe were facilely obtained through the charge transfer approach from the MI precursors LMI(tol) (M = Co, Fe; L = CH(MeC=NDipp)2, Dipp = 2,6-iPr2C6H3) to the diazofluorenylidene-substituted phosphaalkene 1. Structural, magnetic and computational studies on 1Co and 1Fe indicate a weak antiferromagnetic interaction between the high-spin MII ion and the phosphorus radical, resulting in a triplet and quartet ground state, respectively. Complexes 1Co and 1Fe represent the first examples of phosphorus radical coordinated transition metal complexes synthesized via charge transfer, providing a new approach to access radicals of heavier main-group elements...
August 11, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28800556/supplementation-of-lycopene-in-maturation-media-improves-bovine-embryo-quality-in%C3%A2-vitro
#6
M M R Chowdhury, Byung-Hyun Choi, Imran Khan, Kyeong-Lim Lee, Ayman Mesalam, Seok-Hwan Song, Lianguang Xu, Myeong-Don Joo, Fahmida Afrin, Il-Keun Kong
This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P < 0.05) higher, the intracellular ROS concentrations reduced significantly (P < 0.05) in oocytes/blastocysts, TUNEL assay demonstrates reduced apoptosis and increased total cell number per blastocyst (P < 0...
August 3, 2017: Theriogenology
https://www.readbyqxmd.com/read/28794108/plk1-regulates-spindle-association-of-phosphorylated-eukaryotic-translation-initiation-factor-4e-binding-protein-and-spindle-function-in-mouse-oocytes
#7
Ashley L Severance, Keith E Latham
Oocyte meiotic spindles are associated with spindle-enriched mRNAs, phosphorylated ribosome protein S6, and phosphorylated variants of the key translational regulator EIF4EBP1, consistent with translational control of localized mRNAs by EIF4EBP1 in facilitating spindle formation and stability. Using specific kinase inhibitors, we determined which kinases regulate phosphorylation status of EIF4EBP1 associated with meiotic spindles in mouse oocytes, and effects of kinase inhibition on chromosome congression and spindle formation...
August 9, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28792085/creation-of-optically-pure-crystals-from-a-meso-type-gold-i-metalloligand-with-d-and-l-amino-acids-a-coordination-trick
#8
Takuma Itai, Tatsuhiro Kojima, Naoto Kuwamura, Takumi Konno
A unique example of a coordination system that creates optically pure crystals from a meso compound with D- and L-amino acids is reported. The 1:1 reaction of a newly prepared meso digold(I) complex, [Au₂(dcpe)(D-Hpen)(L-Hpen)] ([H₂1]), with Co(OAc)₂ under aerobic conditions yielded a cationic AuI₂CoIII trinuclear complex, [Au₂Co(dcpe)(D-pen)(L-pen)]+ [2]+, in which [1]²- acts as a hexadentate-N2,O2,S2 metalloligand to a CoIII center. Similar reactions with M(OAc)₂ (M = Ni and Zn) produced analogous but neutral AuI₂MII complexes, [Au₂M(dcpe)(D-pen)(L-pen)] ([3M])...
August 9, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28781257/-possible-involvement-of-neuronal-nicotinic-acetylcholine-receptors-in-compensatory-brain-mechanisms-at-early-stages-of-parkinson-s-disease
#9
E V Kryukova, I V Shelukhina, A A Kolacheva, A Kh Alieva, M I Shadrina, P A Slominsky, I E Kasheverov, Y N Utkin, M V Ugrumov, V I Tsetlin
A role of nicotinic acetylcholine receptors (nAChR) in the development of Parkinson's disease (PD) has been investigated using two mouse models corresponding to the presymptomatic stage and the early symptomatic stage of PD. Quantitative determination of nAChR in the striatum and substantia nigra (SN) was performed using the radioactive derivatives of epibatidine, -conotoxin MII, and -bungarotoxin as ligands. The number of ligand-binding sites changed differently depending on their location in the brain, the stage of the disease and the receptor subtype...
May 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/28774739/are-there-any-differences-between-antagonist-administration-on-days-6-and-%C3%A2-6-of-coh-on-assisted-reproductive-technique-outcomes
#10
Zeynep Ozturk Inal, Nafiye Yilmaz, Hasan Ali Inal, Necati Hancerliogullari, Bugra Coskun
BACKGROUND: The aim of this study was to investigate the cost-effectiveness of antagonist administration on stimulation on days <6 and ≥6 of COH on assisted reproductive technique (ART) outcomes. METHODS: In this retrospective cohort study, 412 patients who were admitted to the ART Department were evaluated. In group 1 (203 patients), antagonist administration was provided on days <6 of COH. For group 2 (209 patients), antagonist administration was provided on days ≥6 of COH...
July 31, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28761578/esophageal-motility-and-rikkunshito-treatment-for-proton-pump-inhibitor-refractory-nonerosive-reflux-disease-a-prospective-uncontrolled-open-label-pilot-study-trial
#11
Takeo Odaka, Shigeru Yamato, Osamu Yokosuka
BACKGROUND: Only a few reports focused on esophageal motility in patients with proton pump inhibitor (PPI)-refractory nonerosive reflux disease (NERD) and there has been no established strategy for treatment. OBJECTIVE: To clarify the characteristics of esophageal motility in patients with PPI-refractory NERD, we evaluated esophageal function using combined multichannel intraluminal impedance and esophageal manometry (MII-EM). In addition, we evaluated the efficacy of rikkunshito (RKT), which is a gastrointestinal prokinetic agent...
2017: Current Therapeutic Research, Clinical and Experimental
https://www.readbyqxmd.com/read/28750938/impact-of-cryopreservation-method-on-dromedary-camel-ovary-structure-viability-and-development-of-antral-follicular-oocytes
#12
M M Madboly, E S Abdel-Aal, Eitedal H Elsayed
The objectives of this study were, a) to compare two different vitrification techniques, the solid surface vitrification (SSV) and direct vitrification (DV) method, b) to evaluate the effect of four cryoprotectant agents and their toxicity on the morphological appearance and ultrastructural of camel ovarian cortex, c) to examine the development of oocytes recovered from the vitrified ovarian cortex. Fragments of ovarian cortex were exposed to equilibration solution consisting of TCM- 199 with 10% fetal camel serum (FCS); 0...
July 19, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28750491/severe-delayed-gastric-emptying-induces-non-acid-reflux-up-to-proximal-esophagus-in-neurologically-impaired-patients
#13
Shinji Ishii, Suguru Fukahori, Kimio Asagiri, Yoshiaki Tanaka, Nobuyuki Saikusa, Naoki Hashizume, Motomu Yoshida, Daisuke Masui, Naoko Komatsuzaki, Naruki Higashidate, Saki Sakamoto, Tomohiro Kurahachi, Shiori Tsuruhisa, Hirotomo Nakahara, Minoru Yagi
Background/Aims: The aim of this study is to investigate the degree of delayed gastric emptying (DGE) and evaluate how the severity of DGE affects gastroesophageal reflux disease (GERD) in neurologically impaired (NI) patients utilizing 24-hour multichannel intraluminal impedance pH measurements (pH/MII) and (13)C-acetate breath test ((13)C-ABT) analyses. Methods: (13)C-ABT and pH/MII were conducted in 26 NI patients who were referred to our institution due to suspected GERD...
July 28, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28740669/impact-of-thoracic-surgery-on-esophageal-motor-function-evaluation-by-high-resolution-manometry
#14
Anja Wäsche, Arne Kandulski, Peter Malfertheiner, Sandra Riedel, Patrick Zardo, Thomas Hachenberg, Jens Schreiber
BACKGROUND: Alteration of esophageal function is a potential risk factor for postoperative complications in thoracic surgery. This prospective study investigates esophageal motility and function during and after thoracic procedures via high resolution manometry (HRM) and impedance technology with spatiotemporal representation of pressure data. METHODS: Twelve consecutive patients eligible for elective thoracic surgery underwent preoperative and postoperative (48 hours and 7 days) esophageal HRM...
June 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28733801/effect-of-follicular-flushing-on-reproductive-outcomes-in-patients-with-poor-ovarian-response-undergoing-assisted-reproductive-technology
#15
Anna L M Souza, Marcos Sampaio, Graciele B Noronha, Ludiana G R Coster, Roberta S G de Oliveira, Selmo Geber
PURPOSE: The purpose of this study is to investigate the impact of follicular flushing on the number of oocytes retrieved, oocyte maturity, fertilization rate, embryo development, and pregnancy rate of poor ovarian responders (POR). METHODS: Retrospective study of 524 cycles of 384 patients with POR submitted to assisted reproductive technology (ART) and who had follicular flushing during oocyte retrieval was used in the study. We included patients with <5 oocytes at oocyte retrieval (POR group) and matching the Bologna criteria...
July 21, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28733345/genes-of-cellular-components-of-morphogenesis-in-porcine-oocytes-before-and-after-ivm
#16
Joanna Budna, Artur Bryja, Piotr Celichowski, Rotem Kahan, Wiesława Kranc, Sylwia Ciesiółka, Marta Rybska, Sylwia Borys, Michal Jeseta, Dorota Bukowska, Paweł Antosik, Klaus Peter Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
Proper oocyte maturation in mammals produces an oocyte capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding an oocyte, play a significant role in oocyte maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cumulus cells (CCs) form a dispersed structure, permanent biochemical and molecular modifications occur in the maturing oocytes, indicating the gene expression between immature and mature oocytes differs significantly...
July 21, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28731842/role-of-pepsin-and-oropharyngeal-ph-monitoring-to-assess-the-postoperative-outcome-of-patients-with-laryngopharyngeal-reflux-results-of-a-pilot-trial
#17
Michael Weitzendorfer, Richard Pfandner, Stavros A Antoniou, Werner Langsteger, Kai Witzel, Klaus Emmanuel, Oliver O Koch
BACKGROUND: The aim of this study was to evaluate the value of salivary pepsin and oropharyngeal pH-monitoring to assess the surgical outcome of patients with laryngopharyngeal reflux (LPR). MATERIALS AND METHODS: Twenty consecutive patients with LPR despite proton pump inhibitor treatment received laparoscopic antireflux surgery. Twenty-four hour esophageal pH-monitoring (multichannel intraluminal impedance monitoring [MII]-pH) and esophageal manometry (high-resolution manometry) data were documented preoperatively and at 3-month follow-up...
July 21, 2017: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/28730668/recurrent-triploid-digynic-conceptions-and-mature-ovarian-teratomas-are-they-different-manifestations-of-the-same-genetic-defect
#18
Yassemine Khawajkie, William Buckett, Ngoc Minh Phuong Nguyen, Nawel Mechtouf, Asangla Ao, Jocelyne Arseneau, Rima Slim
Miscarriages affect 15% of clinically recognized pregnancies. Recurrent miscarriage (RM) is defined by the occurrence of at least two consecutive pregnancy losses and affects 1% to 5% of couples trying to conceive. In an attempt to categorize patients with RM and identify the mechanisms leading to their miscarriages, we first used flow cytometry to assess the ploidy of 93 products of conception (POCs) from 53 patients with RM (≥ 3 miscarriages). We identified a single patient with four triploid POCs. We then used fluorescent in situ hybridization to confirm the triploidies and fluorescent microsatellite genotyping with distal and pericentromeric markers to determine their parental origin and the mechanisms leading to their formation...
July 21, 2017: Genes, Chromosomes & Cancer
https://www.readbyqxmd.com/read/28719824/supplementation-of-l-carnitine-during-in%C3%A2-vitro-maturation-improves-embryo-development-from-less-competent-bovine-oocytes
#19
Drahomira Knitlova, Pavlina Hulinska, Michal Jeseta, Katerina Hanzalova, Bartosz Kempisty, Marie Machatkova
The present study was designed to define the impact of l-carnitine, supplemented during maturation, on bovine oocytes with different meiotic competence in terms of their IVF outcomes. Meiotically more competent (MMC) and less competent (MLC) oocytes were obtained separately from differently sized follicles at selected phases of folliculogenesis. The oocytes were matured with or without l-carnitine, fertilized and cultured to the blastocyst stage. The oocytes were examined for nuclear maturation, mitochondrial cluster formation, lipid consumption, fertilization and embryo development...
July 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28717067/barthel-index-and-outcome-of-open-bypass-for-critical-limb-ischemia
#20
Shinsuke Mii, Atsushi Guntani, Eisuke Kawakubo, Hirofumi Shimazoe
BACKGROUND: Daily activity should be an important factor to consider when deciding on a treatment strategy for critical limb ischemia (CLI), and we hypothesized that there was a close relationship between activities of daily living (ADL) and prognosis. The aim of this study was to investigate the association between ADL and outcomes of open bypass for CLI.Methods and Results:A total of 226 patients undergoing infrainguinal bypass for CLI between January 2005 and December 2015 were reviewed...
July 15, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
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