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https://www.readbyqxmd.com/read/29243795/the-effect-on-clinical-results-of-adding-recombinant-lh-in-late-phase-of-ovarian-stimulation-of-patients-with-repeated-implantation-failure-a-pilot-study
#1
A Rahman, D Francomano, F Sagnella, F Lisi, C Manna
OBJECTIVE: To evaluate the efficacy of recombinant LH (r-LH) addition in the late phase of ovarian stimulation in patients with repeated implantation failure (RIF). PATIENTS AND METHODS: 66 infertile couples undergoing ICSI treatment due to male factor were allocated to group A (33) and to group B (33). Group A (29 subjects) received recombinant FSH (r-FSH) supplemented by r-LH in the late follicular phase starting the same day of GnRH-antagonist (GnRH-ant) administration, and group B (32 subjects) received r-FSH alone...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29234093/crispr-cas9-microinjection-in-oocytes-disables-pancreas-development-in-sheep
#2
Marcela Vilarino, Sheikh Tamir Rashid, Fabian Patrik Suchy, Bret Roberts McNabb, Talitha van der Meulen, Eli J Fine, Syed Ahsan, Nurlybek Mursaliyev, Vittorio Sebastiano, Santiago Sain Diab, Mark O Huising, Hiromitsu Nakauchi, Pablo J Ross
One of the ultimate goals of regenerative medicine is the generation of patient-specific organs from pluripotent stem cells (PSCs). Sheep are potential hosts for growing human organs through the technique of blastocyst complementation. We report here the creation of pancreatogenesis-disabled sheep by oocyte microinjection of CRISPR/Cas9 targeting PDX1, a critical gene for pancreas development. We compared the efficiency of target mutations after microinjecting the CRISPR/Cas9 system in metaphase II (MII) oocytes and zygote stage embryos...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29229450/gastric-emptying-time-esophageal-ph-impedance-parameters-quality-of-life-and-gastrointestinal-comorbidity-in-obese-children-and-adolescents
#3
Paolo Quitadamo, Letizia Zenzeri, Enza Mozzillo, Irene Cuccurullo, Alba Rocco, Adriana Franzese, Gerardo Nardone, Annamaria Staiano
OBJECTIVES: To provide further evidence regarding the relationship between obesity and gastroesophageal reflux disease (GERD) in children, through the use of 13C-octanoic acid breath test for gastric emptying time (GET) assessment and esophageal multichannel intraluminal impedance pH-testing (MII-pH). STUDY DESIGN: Obese children aged 4-17 years completed a questionnaire investigating reflux symptoms, the presence of functional gastrointestinal disorders, and quality of life...
December 8, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/29229435/developmental-and-cytogenetic-assessments-of-preimplantation-embryos-derived-from-in-vivo-or-in-vitro-matured-human-oocytes
#4
Farzaneh Fesahat, Seyed Mehdi Kalantar, Mohammad Hasan Sheikhha, Hojjatollah Saeedi, Fatemeh Montazeri, Razieh Dehghani Firouzabadi, Mohammad Ali Khalili
Aneuploidy is of great relevance to embryo selection, as it represents one of the important causes of implantation failure. Furthermore, immature oocytes, retrieved during gonadotrophin-stimulated IVF cycles, are generally discarded in clinics; whereas, there was no detectable comprehensive evidence on higher rates of aneuploidy based on maturity status on the day of oocyte retrieval. As well, the correlation between embryo morphology on aneuploidy remains unclear. The aim was to evaluate the developmental and genetic integrity of human preimplantation embryos from rescue in-vitro matured MII stage oocytes as well as in vivo matured oocytes...
December 8, 2017: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29228123/role-of-yes-associated-protein-1-angiomotin-and-mitogen-activated-kinase-kinase-1-2-in-development-of-the-bovine-blastocyst
#5
Verónica M Negrón-Pérez, Peter J Hansen
The morula-stage embryo is transformed into a blastocyst composed of epiblast, hypoblast and trophectoderm (TE) through mechanisms that, at least in the mouse, involve the Hippo signaling and mitogen-activated kinase (MAPK) pathways. Using the cow as an additional model, we tested the hypotheses that TE and hypoblast differentiation were regulated by the Hippo pathway regulators, YAP1 and AMOT, and MAPK kinase 1/2 (MAPK1/2). The presence of YAP1 and CDX2 in the nucleus and cytoplasm of MII oocytes and embryos was evaluated by immunofluorescence labeling...
December 8, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29215008/roles-of-two-types-of-heparan-sulfate-clusters-in-wnt-distribution-and-signaling-in-xenopus
#6
Yusuke Mii, Takayoshi Yamamoto, Ritsuko Takada, Shuji Mizumoto, Makoto Matsuyama, Shuhei Yamada, Shinji Takada, Masanori Taira
Wnt proteins direct embryonic patterning, but the regulatory basis of their distribution and signal reception remain unclear. Here, we show that endogenous Wnt8 protein is distributed in a graded manner in Xenopus embryo and accumulated on the cell surface in a punctate manner in association with "N-sulfo-rich heparan sulfate (HS)," not with "N-acetyl-rich HS". These two types of HS are differentially clustered by attaching to different glypicans as core proteins. N-sulfo-rich HS is frequently internalized and associated with the signaling vesicle, known as the Frizzled/Wnt/LRP6 signalosome, in the presence of Wnt8...
December 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/29212962/effects-of-insulin-like-growth-factor-1-on-the-in-vitro-maturation-of-canine-oocytes
#7
Akane Sato, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Asuka Hara, Khurchabiling Atchalalt, Naoko Sugane, Rika Fukumori, Yoshikazu Nagao
The maturation rate of canine oocytes during in vitro maturation (IVM) needs to be improved. The present study was designed to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the IVM of canine oocytes. Ovaries were obtained by ovariohysterectomy and were sliced to release cumulus-oocyte complexes (COCs). In Experiment 1, the effects of different concentrations of IGF-1 on the nuclear maturation of oocytes was investigated. The COCs were cultured in a modified medium (mTCM199) with IGF-1 (0, 0...
December 7, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29205619/bapta-am-dramatically-improves-maturation-and-development-of-bovine-oocytes-from-grade-3-cumulus-oocyte-complexes
#8
Hongmei Hu, Xianhong Mo, Xue Li, Xiangwei Fu, Yunpeng Hou
Intracellular free calcium ([Ca2+ ]i ) is essential for oocyte maturation and early embryonic development. Here, we investigated the role of [Ca2+ ]i in oocytes from cumulus-oocyte complexes (COCs) with respect to maturation and early embryonic development, using the calcium-buffering agent BAPTA-AM (1,2-bis[2-aminophenoxy]ethane-N,N,N',N'-tetraacetic acid tetrakis [acetoxymethyl ester]). COCs were graded based on compactness of the cumulus mass and appearance of the cytoplasm, with Grade 1 indicating higher quality and developmental potential than Grade 3...
December 4, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29205220/influence-of-modified-muscle-morphology-and-activity-pattern-on-the-results-of-musculoskeletal-system-modelling-in-cerebral-palsy-patient
#9
Justyna Ogrodnik, Szczepan Piszczatowski
PURPOSE: The aim of the present study was to evaluate the influence of modified morphological parameters of the muscle model and excitation pattern on the results of musculoskeletal system numerical simulation in a cerebral palsy patient. METHODS: The modelling of the musculoskeletal system was performed in the AnyBody Modelling System. The standard model (MoCap) was subjected to modifications consisting of changes in morphological parameters and excitation patterns of selected muscles...
2017: Acta of Bioengineering and Biomechanics
https://www.readbyqxmd.com/read/29204869/estrogen-treatment-in-infertile-women-with-premature-ovarian-insufficiency-in-transitional-phase-a-retrospective-analysis
#10
Sara Pinelli, Paolo Giovanni Artini, Stefano Basile, Maria Elena Rosa Obino, Claudia Sergiampietri, Diana Giannarelli, Giovanna Simi, Vito Cela
PURPOSE: During the transitional phase of premature ovarian insufficiency (POI), sporadic resumption of ovulation is possible because of fluctuation of hormonal levels but the chance of spontaneous pregnancy is low, and the main perspective of childbearing in these women is egg donation or adoption. The purpose of the study was to verify whether treatment with estrogens in POI patients in transitional phase could reduce FSH levels and to evaluate if this pre-treatment could improve reproductive outcomes of in vitro fertilization (IVF)...
December 5, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29201664/vitrification-of-mouse-mii-oocyte-decreases-the-mitochondrial-dna-copy-number-tfam-gene-expression-and-mitochondrial-enzyme-activity
#11
Mahboobeh Amoushahi, Mojdeh Salehnia, Seyed Javad Mowla
Background: The objective of this study was determination of the changes in the reactive oxygen species (ROS) level, mitochondrial DNA (mtDNA) copy number and enzyme activity and transcription factor A (TFAM) gene expression in oocytes after vitrification. Methods: The oocytes at metaphase II (MII) stage (n=320) were collected from super-ovulated adult female mice (n=40). These oocytes were divided into vitrified and non-vitrified groups (n=160 in each group). After vitrification of oocytes, ROS level, mtDNA copy number; TFAM gene expression and mitochondrial enzymes activity (cytochrome C oxidase and succinate dehydrogenase) were assessed and compared with non-vitrified group...
October 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/29199161/indications-of-24-h-esophageal-ph-monitoring-capsule-ph-monitoring-combined-ph-monitoring-with-multichannel-impedance-esophageal-manometry-radiology-and-scintigraphy-in-gastroesophageal-reflux-disease
#12
Rukiye Vardar, Muharrem Keskin
Ambulatory esophageal pH monitoring is an essential method in patients exhibiting signs of non-erosive reflux disease (NERD) to make an objective diagnosis. Intra-esophageal pH monitoring is important in patients who are non-responsive to medications and in those with extraesophageal symptoms, particularly in NERD, before surgical interventions. With the help of the wireless capsule pH monitoring, measurements can be made under more physiological conditions as well as longer recordings can be performed because the investigation can be better tolerated by patients...
December 2017: Turkish Journal of Gastroenterology: the Official Journal of Turkish Society of Gastroenterology
https://www.readbyqxmd.com/read/29184264/effects-of-subacute-administration-of-co-trimoxazole-and-folic-acid-on-ovarian-tissue-in-adult-female-rats
#13
Arezoo Saberi, Ehsan Salarkia, Zohreh Safi, Gholamreza Sepehri
Background: Previous studies have reported the antifertility activities of sulfonamides. This study was designed to evaluate the effects of co-trimoxazole and its co-administration with folic acid on ovarian tissue in female rats. Methods: A total of 54 rats were randomly divided into 9 groups (n=6). Group I served as the control and group II (vehicle) received saline. Other groups, III to IX, received co-trimoxazole (30, 60, and 120 mg/kg; i.p.), folic acid (1 mg/kg; i...
November 2017: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29179787/sperm-derived-factors-enhance-the-in-vitro-developmental-potential-of-haploid-parthenotes
#14
Ramya Nair, Shahin Aboobacker, Srinivas Mutalik, Guruprasad Kalthur, Satish Kumar Adiga
Parthenotes are characterized by poor in vitro developmental potential either due to the ploidy status or the absence of paternal factors. In the present study, we demonstrate the beneficial role of sperm-derived factors (SDF) on the in vitro development of mouse parthenotes. Mature (MII) oocytes collected from superovulated Swiss albino mice were activated using strontium chloride (SrCl2) in the presence or absence of various concentrations of SDF in M16 medium. The presence of SDF in activation medium did not have any significant influence on the activation rate...
November 28, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29177246/the-effect-of-24-hours-delay-in-oocyte-maturation-triggering-in-ivf-icsi-cycles-with-antagonist-protocol-and-not-elevated-progesterone-a-randomized-control-trial
#15
Robab Davar, Elham Naghshineh, Nosrat Neghab
Background: The best time of final oocyte maturation triggering in assisted reproduction technology protocols is unknown. This time always estimated by combined follicular size and blood progesterone level. Objective: The aim of this study was evaluation of the effect of delaying oocyte maturation triggering by 24 hr on the number of mature oocytes (MII) and other in vitro fertilization cycle characteristics in antagonist protocols with not-elevated progesterone (p ≤1 ng/ml)...
July 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29172789/morphometric-analysis-of-human-oocytes-using-time-lapse-does-it-predict-embryo-developmental-outcomes
#16
Azita Faramarzi, Mohammad Ali Khalili, Marjan Omidi
The aim of this prospective study was to evaluate the relationship between morphometric parameters of metaphase II (MII) oocytes and the morphokinetic behaviour of subsequent embryos derived by intra-cytoplasmic sperm injection (ICSI). The association between oocyte morphometry: (whole oocyte), ooplasm, width of zona pellucida (ZP) and perivitelline space (PVS) and first polar body (PB) with embryo morphokinetic variables, including time of second PB extrusion (tPB2), pronuclei appearance (tPN), pronuclei fading (tPNf), formation of two to eight cells (t2 to t8) and irregular cleavage events [uneven at two cells stage, cell fusion (Fu) and trichomonas mitoses (TM)] were assessed...
November 24, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29166876/gastroesophageal-flap-valve-reflected-egj-morphology-and-correlated-to-acid-reflux
#17
Chenxi Xie, Yuwen Li, Ning Zhang, Lishou Xiong, Minhu Chen, Yinglian Xiao
OBJECTIVE: The anatomy of esophagogastric junction (EGJ) serves as the anti-reflux barrier. The gastroesophageal flap valve (GEFV) is a component of EGJ. The aim of the current study was to assess its correlation with the esophageal acid exposure and the impact on anti-reflux barrier function by using the metrics of EGJ contraction. METHODS: Eighty three patients with typical GERD symptoms were included in the study. Upper endoscopy, high-resolution manometry (HRM) and 24 h multichannel intraluminal impedance-pH (MII-pH) monitoring were performed in all patients...
November 22, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/29161810/chemical-cosubstitution-oriented-design-of-rare-earth-borates-as-potential-ultraviolet-nonlinear-optical-materials
#18
Miriding Mutailipu, Zhiqing Xie, Xin Su, Min Zhang, Ying Wang, Zhihua Yang, Muhammad Ramzan Saeed Ashraf Janjua, Shilie Pan
Chemical cosubstitution strategy was implemented to design the potential ultraviolet (UV) and deep-UV nonlinear optical (NLO) materials. Taking the classic β-BaB2O4 as a maternal structure, by simultaneously replacing the Ba2+ and [B3O6]3- units for monovalant (K+), divalent (alkaline earth metal), trivalent (rare-earth metal, Bi3+) ions, and the [B5O10]5- clusters through two different practical routes, altogether twelve new mixed-metal noncentrosymmetric borates K7MIIRE2(B5O10)3 (MII = Ca, Sr, Ba, K/RE0...
November 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29145719/maintained-mpf-level-after-oocyte-vitrification-improves-embryonic-development-after-ivf-but-not-after-somatic-cell-nuclear-transfer
#19
Ji I Baek, Dong-Won Seol, Ah-Reum Lee, Woo Sik Lee, Sook-Young Yoon, Dong Ryul Lee
Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. Here, we evaluated MPF activity in vitrified mouse eggs following treatment with caffeine, a known stimulator of MPF activity, and/or the proteasome inhibitor MG132. Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG)...
November 16, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29145066/relationship-between-follicular-dynamics-and-oocyte-maturation-during-in%C3%A2-vitro-culture-as-a-non-invasive-sign-of-caprine-oocyte-meiotic-competence
#20
J Cadenas, C Maside, A C A Ferreira, L A Vieira, J Leiva-Revilla, V M Paes, B G Alves, F Z Brandão, A P R Rodrigues, M B Wheeler, J R Figueiredo
The search for non-invasive signs of oocyte meiotic competence is very important for the development of in vitro follicle culture (IVFC) systems. The aims of the present study were: (1) to investigate the effect of in vitro maturation (IVM) of in vivo grown goat COCs, in group or individually, on oocyte chromatin configuration (Experiment 1), and (2) the influence of IVFC period (12 vs. 18 days) on the ability of the oocyte to resume meiosis immediately after IVFC (before in vitro maturation; IVM), or after IVM (Experiment 2)...
November 4, 2017: Theriogenology
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