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https://www.readbyqxmd.com/read/28543962/effects-of-meiotic-inhibitors-and-gonadotrophins-on-porcine-oocytes-in-vitro-maturation-fertilization-and-development
#1
M A Gil, A Nohalez, C A Martinez, J R Ake-Villanueva, F Centurion-Castro, C Maside, C Cuello, J Roca, I Parrilla, E A Martinez
This study evaluated the effect of three reversible meiotic inhibitors (MINs) and their interaction with gonadotrophins (Gns) on the meiotic maturation and developmental competence of porcine oocytes. In experiment 1, the oocytes were matured for 22 hr in the presence or absence of dbcAMP (1 mM), cycloheximide (7 μM) or cilostamide (20 μM) with or without Gns, and for an additional 22 hr in the absence of MINs and Gns. At 22 hr of maturation, regardless of the presence of Gns, a higher proportion (p < ...
May 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28537683/epigenetic-changes-of-histone-deacetylation-in-murine-oocytes-matured-in-vitro-versus-in-vivo
#2
J Huang, C-H Ding, Z-Y Li, X-B Zhang, Z-S You, C-Q Zhou, Y-W Xu
OBJECTIVE: In this study, we studied changes in histone acetylation during mouse oocytes meiosis. The aim was to investigate HDAC1 expression patterns in the mouse oocytes and effects of in-vitro maturation on epigenetic modifications during meiosis. MATERIALS AND METHODS: Immature and mature oocytes were collected from female Kunming white mice of 4-6 weeks in age. Dynamic changes of histone H3K9, H4K12 acetylation were explored. HDAC1 spatial and temporal expression patterns during meiosis and their expression changes in in-vitro maturation were determined...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28535800/comparison-of-oesophageal-function-tests-between-chinese-non-erosive-reflux-disease-and-reflux-hypersensitivity-patients
#3
Feng Gao, Yan Gao, Xue Chen, Jie Qian, Jie Zhang
BACKGROUND: By means of 24 h multi-channel intraluminal impedance and pH recording (MII/pH), patients with heartburn and normal upper gastrointestinal endoscopy findings can be classified into those with non-erosive reflux disease (NERD) and those with reflux hypersensitivity (RH). Therefore, in this study, we investigated the difference in oesophageal function tests in Chinese patients with NERD and RH. METHODS: NERD patients were selected from the digestive department, Beijing Anzhen Hospital and Beijing Chao-Yang Hospital, Capital Medical University, after upper gastrointestinal endoscope, high-resolution manometry and impedance (HRiM), and MII/pH examinations between 2014 and 2016...
May 23, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28533957/pld2-regulates-microtubule-stability-and-spindle-migration-in-mouse-oocytes-during-meiotic-division
#4
Xiaoyu Liu, Xiaoyun Liu, Dandan Chen, Xiuying Jiang, Wei Ma
Phospholipase D2 (PLD2) is involved in cytoskeletal reorganization, cell migration, cell cycle progression, transcriptional control and vesicle trafficking. There is no evidence about PLD2 function in oocytes during meiosis. Herein, we analyzed PLD2 expression and its relationship with spindle formation and positioning in mouse oocyte meiosis. High protein level of PLD2 was revealed in oocytes by Western blot, which remained consistently stable from prophase I with intact germinal vesicle (GV) up to metaphase II (MII) stage...
2017: PeerJ
https://www.readbyqxmd.com/read/28533401/evolutionary-steps-involving-counterion-displacement-in-a-tunicate-opsin
#5
Keiichi Kojima, Takahiro Yamashita, Yasushi Imamoto, Takehiro G Kusakabe, Motoyuki Tsuda, Yoshinori Shichida
Ci-opsin1 is a visible light-sensitive opsin present in the larval ocellus of an ascidian, Ciona intestinalis This invertebrate opsin belongs to the vertebrate visual and nonvisual opsin groups in the opsin phylogenetic tree. Ci-opsin1 contains candidate counterions (glutamic acid residues) at positions 113 and 181; the former is a newly acquired position in the vertebrate visual opsin lineage, whereas the latter is an ancestral position widely conserved among invertebrate opsins. Here, we show that Glu113 and Glu181 in Ci-opsin1 act synergistically as counterions, which imparts molecular properties to Ci-opsin1 intermediate between those of vertebrate- and invertebrate-type opsins...
May 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28523360/comparison-of-the-multichannel-intraluminal-impedance-ph-and-conventional-ph-for-measuring-esophageal-acid-exposure-a-propensity-score-matched-analysis
#6
Masato Hoshino, Nobuo Omura, Fumiaki Yano, Kazuto Tsuboi, Se Ryung Yamamoto, Shunsuke Akimoto, Takahiro Masuda, Hideyuki Kashiwagi, Katsuhiko Yanaga
BACKGROUND: The modalities for evaluating acid reflux in medical care for gastroesophageal reflux disease (GERD) include conventional pH (C-pH), wireless pH (Bravo(®)) and multichannel intraluminal impedance pH (MII-pH), which have been reported to vary with respect to the duration of acid reflux. In this study, we examined the difference between the acid reflux in C-pH and MII-pH among patients with GERD. METHODS: Prior to initial laparoscopic fundoplication carried out on 297 cases from December 1994 to April 2016, an upper gastrointestinal endoscopy and C-pH or MII-pH were conducted...
May 18, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28522534/21st-century-rise-in-anthropogenic-nitrogen-deposition-on-a-remote-coral-reef
#7
Haojia Ren, Yi-Chi Chen, Xingchen T Wang, George T F Wong, Anne L Cohen, Thomas M DeCarlo, Mira A Weigand, Horng-Sheng Mii, Daniel M Sigman
With the rapid rise in pollution-associated nitrogen inputs to the western Pacific, it has been suggested that even the open ocean has been affected. In a coral core from Dongsha Atoll, a remote coral reef ecosystem, we observe a decline in the (15)N/(14)N of coral skeleton-bound organic matter, which signals increased deposition of anthropogenic atmospheric N on the open ocean and its incorporation into plankton and, in turn, the atoll corals. The first clear change occurred just before 2000 CE, decades later than predicted by other work...
May 19, 2017: Science
https://www.readbyqxmd.com/read/28518085/analysis-of-chromosome-segregation-histone-acetylation-and-spindle-morphology-in-horse-oocytes
#8
Federica Franciosi, Irene Tessaro, Rozenn Dalbies-Tran, Cecile Douet, Fabrice Reigner, Stefan Deleuze, Pascal Papillier, Ileana Miclea, Valentina Lodde, Alberto M Luciano, Ghylene Goudet
The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice...
May 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516917/maternal-age-dependent-apc-c-mediated-decrease-in-securin-causes-premature-sister-chromatid-separation-in-meiosis-ii
#9
Ibtissem Nabti, Rosanna Grimes, Hema Sarna, Petros Marangos, John Carroll
Sister chromatid attachment during meiosis II (MII) is maintained by securin-mediated inhibition of separase. In maternal ageing, oocytes show increased inter-sister kinetochore distance and premature sister chromatid separation (PSCS), suggesting aberrant separase activity. Here, we find that MII oocytes from aged mice have less securin than oocytes from young mice and that this reduction is mediated by increased destruction by the anaphase promoting complex/cyclosome (APC/C) during meiosis I (MI) exit. Inhibition of the spindle assembly checkpoint (SAC) kinase, Mps1, during MI exit in young oocytes replicates this phenotype...
May 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/28502309/effects-of-vitrification-of-cumulus-enclosed-porcine-oocytes-at-the-germinal-vesicle-stage-on-cumulus-expansion-nuclear-progression-and-cytoplasmic-maturation
#10
Ruth Appeltant, Tamás Somfai, Elisa C S Santos, Thanh Quang Dang-Nguyen, Takashi Nagai, Kazuhiro Kikuchi
Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus-oocyte complexes (COCs) and control COCs (no cryoprotectant treatment and no cooling), as well as a toxicity control (no cooling). Vitrification was performed with 17.5% (v/v) ethylene glycol and 17...
May 15, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28495303/the-characteristics-of-salivary-pepsin-in-patients-with-severe-motor-and-intellectual-disabilities
#11
Naoki Hashizume, Suguru Fukahori, Kimio Asagiri, Shinji Ishii, Nobuyuki Saikusa, Naruki Higashidate, Motomu Yoshida, Daisuke Masui, Saki Sakamoto, Yoshiaki Tanaka, Minoru Yagi, Yushiro Yamashita
PURPOSE: The aim of the present study was to determine the utility of measuring the salivary pepsin level (SPL) as an objective assessment of gastroesophageal reflux disease (GERD) in severe motor and intellectual disabilities (SMID) patients. SUBJECTS AND METHODS: This prospective study included 26 SMID patients who underwent simultaneous 24-h multichannel intraluminal impedance pH measurement (pH/MII) and SPL evaluation. The enrolled patients were divided into GERD (+) or GERD (-) groups according to the pH/MII findings...
May 8, 2017: Brain & Development
https://www.readbyqxmd.com/read/28466179/is-there-any-correlation-between-oocyte-polarization-microscopy-findings-with-embryo-time-lapse-monitoring-in-icsi-program
#12
Azita Faramarzi, Mohammad Ali Khalili, Azam Agha-Rahimi, Marjan Omidi
PURPOSE: The aim was to investigate the relationship between the presence of the meiotic spindle (MS) and zona pellucida (ZP) birefringence of MII oocytes with morphokinetics variables of derived embryos in ICSI setting. METHODS: Using a polarization imaging system, the ZP birefringence and presence of MS were evaluated pre ICSI. Also, morphokinetics variables including time of second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf), time of two to eight discrete cells (t2-t8) ECC1 (t2-tPB2), cc2a (t3-t2), S2 (t4-t3) and S3 (t8-t5) as well as irregular cleavage events of 368 embryos were analyzed with time lapse monitoring (TLM)...
May 2, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28460666/effect-of-oocyte-maturation-time-sperm-selection-method-and-oxygen-tension-on-in%C3%A2-vitro-embryo-development-in-alpacas
#13
Jaime Ruiz, R Paulo Santayana, M José Mendoza, J Leandra Landeo, Elizabeth Huamán, Flamel Ticllacuri, L Fidel Mujica, Mauricio Silva, Marcelo H Ratto
We evaluated the effect of in vitro maturation time, sperm selection and oxygen tension on alpaca embryo development. In Experiment I, Cumulus Oocyte- Complexes (COCs) were obtained from abattoir ovaries and in vitro matured in TCM-199 for 24 (n = 217), 28 (215), or 32 h (223) at 38.5 °C, high humidity and 5% CO2 in air. Oocytes from 24 (n = 392), 28 (n = 456) or 32 (n = 368) h groups were in vitro fertilized with epididymal sperm and cultured in SOFaa at 38.5 °C, high humidity and 5% CO2, 5% O2 and 90% N2 for 7 days...
June 2017: Theriogenology
https://www.readbyqxmd.com/read/28458301/ultrastructural-characteristics-of-human-oocytes-vitrified-before-and-after-in-vitro-maturation
#14
Yolanda Segovia, Noemí Victory, Irene Peinado, Laura M García-Valverde, Magdalena García, Jon Aizpurua, Ana Monzó, María José Gómez-Torres
The development of an effective program that combines in vitro maturation (IVM) and cryopreservation for immature oocytes would represent a novel advance for in vitro fertilization (IVF), especially as a means to preserve the fertility of women in unique situations. The aim of this study was to analyze the ultrastructural characteristics of human oocytes, obtained after controlled ovarian stimulation, to determine whether IVM is best performed before or after vitrification. To this end, we analyzed the following features in a total of 22 MII oocytes: size, zona pellucida and perivitelline space, mitochondria number, M-SER (mitochondria-smooth endoplasmic reticulum) aggregates and M-V (mitochondria-vesicle) complexes, the number of cortical granules and microvilli, and the presence of vacuolization using transmission electron microscopy (TEM)...
April 30, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28457181/new-amh-assay-allows-rapid-point-of-care-measurements-of-ovarian-reserve
#15
Ewa Pastuszek, Aron Lukaszuk, Michal Kunicki, Joanna Mockun, Grzegorz Kloss, Iwona Malinowska, Adam Czyzyk, Blazej Meczekalski, Krzysztof Lukaszuk
In this study, we compare two commercial automated immunoassays used to evaluate serum anti-Müllerian hormone (AMH) levels as a prognostic value for ovarian response and pregnancy outcome in assisted reproductive technology cycles. Serum AMH was measured for 193 women. We performed a simultaneous measurement in serum AMH with the two alternative kits VIDAS® and Elecsys® AMH assay. For all women undergoing in vitro fertilization cycle, we collected data on their antral follicle count (AFC) and numbers of retrieved cumulus oocyte complexes (OC) and metaphase II oocytes and pregnancy outcome...
May 1, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28456957/gene-expression-analysis-in-mammalian-oocytes-and-embryos-by-quantitative-real-time-rt-pcr
#16
Kyeoung-Hwa Kim, Su-Yeon Lee, Kyung-Ah Lee
This chapter describes methods for preparing oocytes and embryos to analyze their gene expression at specific developmental stages. We illustrate how to collect germinal vesicles (GVs) and mature metaphase II (MII) stage oocytes, as well as how to collect embryos at specific developmental stages from the pronucleus (PN) to the blastocyst stage from female mice. We also describe how to prepare mRNAs from these precious cells to analyze the expression of the target genes. The materials and methods in this chapter are used mainly for mouse oocytes and embryos, but with subtle modifications, they may be applicable for most mammalian species...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28455945/the-influence-of-delipidation-on-triglyceride-and-lipin1-of-porcine-embryos-derived-from-parthenogenetic-activation
#17
Y Zeng, C Wang, Y Niu, D Chi, M Xu, L Si, X Qu, J Li
Proteins in the LIPIN family play key roles in lipid synthesis mainly on triacylglycerol (TAG) biosynthesis, and they also act as transcriptional coactivators to regulate the expression of genes involved in lipid metabolism with other nuclear factors. Hence, this study was designed to investigate LIPIN1 in pig oocytes and embryos by the delipidation. After delipidation, the content of lipids (LDs) and TAG in MII oocyte was significantly reduced; however, a similar increasing tendency of TAG was shown during embryos development...
April 29, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28455654/metabolically-inactive-insulin-analogue-does-not-prevent-autoimmune-diabetes-in-nod-mice
#18
Juha Grönholm, Philippe P Pagni, Minh N Pham, Claire B Gibson, Paul F Macomber, José Luis Vela, Matthias von Herrath, Michael J Lenardo
AIMS/HYPOTHESIS: Insulin is widely considered to be a driver antigen in type 1 diabetes in humans and in mouse models of the disease. Therefore, insulin or insulin analogues are candidates for tolerogenic drugs to prevent disease onset in individuals with risk of diabetes. Previous experiments have shown that autoimmune diabetes can be prevented in NOD mice by repeated doses of insulin administered via an oral, nasal or parenteral route, but clinical trials in humans have not succeeded...
April 28, 2017: Diabetologia
https://www.readbyqxmd.com/read/28447513/clinical-utility-of-different-anti-m%C3%A3-llerian-hormone-amh-assays-for-the-purpose-of-pregnancy-prediction
#19
Joanna Liss, Michal Kunicki, Adam Czyzyk, Ewa Pastuszek, Judyta Zabielska, Blazej Meczekalski, Krzysztof Lukaszuk
PURPOSE: Comparison of outcomes of IVF cycles where the AMH levels was measured with five different AMH kits: Immunotech (IOT), Beckman Coulter II Gen. RUO, Beckman Coulter II Gen. IVD (BC II IVD), Ansh Labs ultrasensitive (Ansh) and the automated Elecsys Roche assay. METHODS: Retrospective analysis of clinical data for 3693 cycles. RESULTS: In women < 35 years with low (<0.6 ng/ml) and high (>1.4 ng/ml) AMH concentrations, and in those > 39 years with medium (≥0...
April 27, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28445721/nacho-mediates-nicotinic-acetylcholine-receptor-function-throughout-the-brain
#20
Jose A Matta, Shenyan Gu, Weston B Davini, Brian Lord, Edward R Siuda, Anthony W Harrington, David S Bredt
Neuronal nicotinic acetylcholine receptors (nAChRs) participate in diverse aspects of brain function and mediate behavioral and addictive properties of nicotine. Neuronal nAChRs derive from combinations of α and β subunits, whose assembly is tightly regulated. NACHO was recently identified as a chaperone for α7-type nAChRs. Here, we find NACHO mediates assembly of all major classes of presynaptic and postsynaptic nAChR tested. NACHO acts at early intracellular stages of nAChR subunit assembly and then synergizes with RIC-3 for receptor surface expression...
April 25, 2017: Cell Reports
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