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https://www.readbyqxmd.com/read/29455014/cilostazol-improves-wound-healing-in-patients-undergoing-open-bypass-for-ischemic-tissue-loss-a-propensity-score-matching-analysis
#1
Shinsuke Mii, Atsushi Guntani, Eisuke Kawakubo, Kiyoshi Tanaka, Ryoichi Kyuragi
BACKGROUND: Few reports have described the effects of medication on the wound healing of ischemic ulcers after revascularization. This study was conducted to investigate the effects of cilostazol on wound healing in patients who underwent infrainguinal bypass for ischemic tissue loss. METHODS: Two hundred and sixty-three limbs undergoing de novo infrainguinal bypass for tissue loss from January 2004 to December 2015 were divided into two groups based on whether or not cilostazol was administered after surgery...
February 15, 2018: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/29447173/dynamic-changes-of-setd2-a-histone-h3k36-methyltransferase-in-porcine-oocytes-ivf-and-scnt-embryos
#2
Yun Fei Diao, Tao Lin, Xiaoxia Li, Reza K Oqani, Jae Eun Lee, So Yeon Kim, Dong Il Jin
SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine fetal fibroblasts, oocytes, and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA), and somatic cell nuclear transfer (SCNT)...
2018: PloS One
https://www.readbyqxmd.com/read/29434340/glucocorticoid-receptor-expression-in-resident-and-hematopoietic-cells-in-igg4-related-disease
#3
Taku Iguchi, Koji Takaori, Akiko Mii, Yuki Sato, Yasunori Suzuki, Hajime Yoshifuji, Hiroshi Seno, Osamu Ogawa, Koichi Omori, Kazuhisa Bessho, Satoru Kondo, Tomokazu Yoshizaki, Hitoshi Nakashima, Takao Saito, Tsuneyo Mimori, Hironori Haga, Mitsuhiro Kawano, Motoko Yanagita
Immunoglobulin G4-related disease is a rare immune-mediated disease characterized by the infiltration of IgG4-positive plasma cells and unique storiform fibrosis of multiple organs. The majority of IgG4-related disease patients respond to glucocorticoids, yet the precise mechanism of their action remains unclear. Pathological sections of the submaxillary gland, kidney, and retroperitoneum from 20 patients in total diagnosed with IgG4-related disease were analyzed for glucocorticoid receptor expression and the cell types expressing glucocorticoid receptor...
February 12, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29434078/fas-associated-protein-factor-1-is-involved-in-meiotic-resumption-in-mouse-oocytes
#4
Hui Peng, Jianchao Huo, Yuyun Gao, Jing Chen, Xiang Yu, Tianfang Xiao
Fas-associated protein factor 1 (FAF1) is a Fas-associated protein that functions in multiple cellular processes. Previous research showed that mutations in Faf1 led to the lethality of cleavage stage embryos in a mouse model. The aim of the present study was to analyze the expression pattern, localization, and function of FAF1 in meiotic resumption of mouse oocytes. FAF1 was exclusively expressed in oocytes at various follicular stages within the ovary and was predominantly localized in the cytoplasm of growing oocytes...
February 9, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29430805/the-localization-and-function-of-p38%C3%AE-mitogen-activated-protein-kinase-in-rat-oocytes
#5
S Hu, Q Yu, Y Wang, D Ke, F Zhou, G Cheng, W Xia, C Zhu
P38α mitogen-activated protein kinase (MAPK), which is a member of the canonical MAPK family, is activated in response to various extracellular stresses and plays a role in multiple cellular processes. In this study, we investigated the expression, subcellular localization and functional roles of p38α MAPK during the meiotic maturation of rat oocytes. We found that p38α MAPK phosphorylation (p-p38α MAPK, indicative of p38α MAPK activation) was low at the germinal vesicle (GV) stage, increased 3 hr after germinal vesicle breakdown (GVBD) and maintained its maximum at metaphase I (MI) or metaphase II (MII)...
February 12, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29425439/near-uv-water-splitting-by-cu-ni-and-co-complexes-in-the-gas-phase
#6
Andy Dang, Christopher J Shaffer, Daniel Bim, John Lawler, Michael Lesslie, Victor Ryzhov, Frantisek Turecek
(2,2'-bipyridine)M=O+ ions (M = Cu, Ni, Co) were generated by collision-induced dissociation and near-UV photodissociation of readily available [(2,2'-bipyridine)MII(NO3)]+ ions in the gas phase and their structure was confirmed by ion-molecule reactions combined with isotope labeling. Upon storage in a quadrupole ion trap, the (2,2'-bipyridine)M=O+ ions spontaneously added water, and the formed [(2,2'-bipyridine)M=O + H2O]+ complexes eliminated OH upon further near-UV photodissociation. This reaction sequence can be accomplished at a single laser wavelength in the range of 260-340 nm to achieve stoichiometric homolytic cleavage of gaseous water...
February 9, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29412646/expanding-the-structural-motif-landscape-of-heterometallic-%C3%AE-diketonates-congruently-melting-ionic-solids
#7
Matthew C Barry, Craig M Lieberman, Zheng Wei, Rodolphe Clérac, Alexander S Filatov, Evgeny V Dikarev
The first example of ionic β-diketonates in which both the cation and anion are octahedral coordinatively saturated metal diketonate moieties are reported. Heterometallic tin-transition-metal heteroleptic diketonates were obtained through solid-state redox reactions and are formulated as {[SnIV(thd)3]+[MII(hfac)3]-} (MII = Mn (1), Fe (2), Co (3); thd = 2,2,6,6-tetramethyl-3,5-heptanedionate, hfac = hexafluoroacetylacetonate). X-ray single-crystal structural investigations along with DART mass spectrometry, multinuclear NMR, and magnetic susceptibility measurements have been used to confirm an assignment of metal oxidation states in compounds 1-3...
February 7, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29408640/importance-of-frequency-and-morphological-characteristics-of-nodular-diabetic-glomerulosclerosis-in-diabetic-nephropathy
#8
Fumihiko Yasuda, Akiko Mii, Megumi Morita, Michiko Aoki, Masako Tagawa, Sae Aratani, Tomohiro Kaneko, Yukinao Sakai, Akira Shimizu
The Renal Pathology Society proposed a pathological classification for diabetic nephropathy (DN) (RPS 2010). We retrospectively examined the renal structural-functional relationships using the RPS 2010 classification in 49 DN cases. We also evaluated the importance of the percentage of glomeruli with nodular diabetic glomerulosclerosis and their morphological characteristics (cellular, cellular and extracellular matrix [ECM] or ECM types) in the pathology of DN. The classes of DN (RPS 2010) were significantly correlated with the duration of diabetes mellitus (DM), degree of proteinuria, a decreased estimated glomerular filtration rate (eGFR), and the stages of Japanese clinical DM and chronic kidney disease (CKD)...
February 1, 2018: Human Pathology
https://www.readbyqxmd.com/read/29406725/cation-assisted-reversible-folding-and-anion-binding-by-a-naphthalenediimide-based-ditopic-ion-pair-receptor
#9
Krishnendu Maity, Dillip K Panda, Robert J Gallup, Chandan K Choudhury, Sourav Saha
A novel heteroditopic ion-pair receptor was synthesized by tethering two π-acidic naphthalenediimide units with a tridentate bisimine linker. Upon chelating a ZnII or CdII ion with the linker, it adopts a U-conformation allowing the parallel NDI arms to sandwich charge-diffuse anions, and two such folded receptor molecules interlock with each other forming an [R3·MII·R3·X-] ion-pair complex. Extraction of the guest cation with a tetraazacrown ether returns the receptor to its original unfolded form.
February 6, 2018: Organic Letters
https://www.readbyqxmd.com/read/29405928/structural-identification-of-the-nicotinic-receptor-ion-channel
#10
Jean-Pierre Changeux
The structural identification of the cationic channel of the acetylcholine nicotinic receptor involved its biochemical tagging by channel blockers, followed by site-directed mutagenesis analyses reported in two papers in 1988. The ion channel was demonstrated to be lined by the transmembrane segment MII, in which the binding sites of channel blockers are "framed" by anionic rings.
February 2018: Trends in Neurosciences
https://www.readbyqxmd.com/read/29399800/double-blind-placebo-controlled-study-with-alginate-suspension-for-laryngopharyngeal-reflux-disease
#11
Wen-Hsuan Tseng, Ping-Huei Tseng, Jia-Feng Wu, Ya-Chin Hsu, Ting-Yi Lee, Yen-Hsuan Ni, Hsiu-Po Wang, Tzu-Yu Hsiao, Wei-Chung Hsu
OBJECTIVE: Treatment for laryngopharyngeal reflux disease (LPRD) is challenging because of delays in recognition and poor responsiveness to proton-pump inhibitor therapy. The aim of this study was to determine the efficacy and safety of liquid alginate suspension for treating LPRD. METHODS: A double-blind, placebo-controlled, prospective study comparing 8 weeks of treatment with Alginos Oral Suspension (TTY Biopharm Co. Ltd., Taipei, Taiwan) (sodium alginate 1,000 mg three times daily) with a placebo was conducted on patients who fulfilled the criteria of at least one symptom consistent with LPRD, a total reflux symptom index (RSI) score of > 10, and a total reflux finding score (RFS) of > 5...
February 5, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29397252/freeze-dried-spermatozoa-an-alternative-biobanking-option-for-endangered-species
#12
Debora Agata Anzalone, Luca Palazzese, Domenico Iuso, Giuseppe Martino, Pasqualino Loi
In addition to the iconic wild species, such as the pandas and Siberian tigers, an ever-increasing number of domestic species are also threatened with extinction. Biobanking of spermatozoa could preserve genetic heritages of extinct species, and maintain biodiversity of existing species. Because lyophilized spermatozoa retain fertilizing capacity, the aim was to assess whether freeze-dried spermatozoa are an alternative option to save endangered sheep breeds. To achieve this objective, semen was collected from an Italian endangered sheep breed (Pagliarola), and a biobank of cryopreserved and freeze-dried spermatozoa was established, and evaluated using IVF (for frozen spermatozoa) and ICSI procedures (for frozen and freeze-dried spermatozoa)...
January 31, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29396444/differential-long-non-coding-rna-expression-profiles-in-human-oocytes-and-cumulus-cells
#13
Julien Bouckenheimer, Patricia Fauque, Charles-Henri Lecellier, Céline Bruno, Thérèse Commes, Jean-Marc Lemaître, John De Vos, Said Assou
Progress in assisted reproductive technologies strongly relies on understanding the regulation of the dialogue between oocyte and cumulus cells (CCs). Little is known about the role of long non-coding RNAs (lncRNAs) in the human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that were abundant in metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, and LINC01118) and CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, and VIM-AS1)...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29393167/evaluation-of-esophageal-mucosal-integrity-in-patients-with-gastroesophageal-reflux-disease
#14
Tomoaki Matsumura, Makoto Arai, Hideaki Ishigami, Mai Fujie, Kentaro Ishikawa, Naoki Akizue, Takashi Taida, Yuki Ohta, Shinsaku Hamanaka, Kenichiro Okimoto, Keiko Saito, Daisuke Maruoka, Tomoo Nakagawa, Naoya Kato
BACKGROUND: Impaired esophageal mucosal integrity plays a role in causing symptoms of gastroesophageal reflux disease (GERD). Recently, the assessment of esophageal baseline impedance (BI) using the multichannel intraluminal impedance-pH (MII-pH) test was suggested as a surrogate technique for the study of esophageal mucosal integrity and was reported to be useful in distinguishing GERD from non-GERD. However, measuring BI requires a 24-h testing period, is complicated, and causes considerable patient discomfort...
February 1, 2018: Digestion
https://www.readbyqxmd.com/read/29376631/one-step-preparation-of-highly-hydrophobic-and-oleophilic-melamine-sponges-via-metal-ion-induced-wettability-transition
#15
Yichun Ding, Wenhui Xu, Ying Yu, Haoqing Hou, Zhengtao Zhu
Hydrophobic and oleophilic absorbent materials have received wide attentions in recent years for potential applications in pollutant removal from accidental spills of oil or organic chemicals. In this work, we report a Metal-Ion Induced Hydrophobic Melamine Sponge (MII-HMS) prepared by a one-step solution immersion process. The commercial melamine sponge (intrinsically superhydrophilic with a water contact angle of ~ 0ο) is immersed in an aqueous solution of transition metal ions (e.g., FeCl3, Fe(NO3)3, Zn(NO3)2, Ni(NO3)2, and Co(NO3)2) for a short period, followed by drying...
January 29, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29371829/ultrasound-guided-laparotomic-oocyte-retrieval-during-surgery-for-fertility-preservation-in-a-case-of-tumor-recurrence-after-a-unilateral-salpingo-oophorectomy
#16
Ayumu Ito, Yukiko Katagiri, Yusuke Fukuda, Tsuyoki Kugimiya, Koichi Nagao, Mineto Morita
Case: A 28 year old unmarried woman underwent a unilateral salpingo-oophorectomy and was suspected of having a malignant tumor in the remaining ovary. After consultation with the patient and her family, it was decided to cryopreserve the unfertilized oocytes. In order to reduce the risk of puncturing or rupturing the tumor when performing the oocyte retrieval from the ovary that was affected by the malignant tumor, it was chosen to use direct laparotomic oocyte retrieval during surgery, instead of conventional transvaginal retrieval...
January 2018: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29371050/laparoscopic-transabdominal-preperitoneal-approach-for-giant-inguinal-hernias
#17
Ryosuke Fujinaka, Takeshi Urade, Eiji Fukuoka, Koichi Murata, Yasuhiko Mii, Hidehiro Sawa, Mariko Man-I, Shigeteru Oka, Yoshiteru Iwatani, Daisuke Kuroda
BACKGROUND: Many surgical techniques have been developed to treat inguinal hernia. In recent years, the laparoscopic transabdominal preperitoneal (TAPP) approach has been widely performed to repair inguinal hernia. Giant inguinal hernia (GIH) is an extremely rare disease that is a challenge for general surgeons. GIH appears when patients neglect the treatment for many years and it is defined as an inguinal hernia that extends below the midpoint of inner thigh in standing position. According to previous publications, the Lichtenstein tension-free hernioplasty is recommended to repair GIH...
January 20, 2018: Asian Journal of Surgery
https://www.readbyqxmd.com/read/29363690/new-complex-phosphates-cs3miibi-p2o7-2-mii-ca-sr-and-pb-synthesis-characterization-crystal-and-electronic-structure
#18
Igor V Zatovsky, Nataliia Yu Strutynska, Yuriy A Hizhnyi, Vyacheslav N Baumer, Ivan V Ogorodnyk, Nikolay S Slobodyanik, Ievgen V Odynets, N I Klyui
Herein, the peculiarities of complex phosphate formation in self-fluxes of Cs-MII-Bi-P-O (MII = Ca, Sr, Ba and Pb) systems with Cs/P = 0.7-1.3 at fixed ratios of Bi/P = 0.2 and Bi/MII = 1.0 were studied and discussed. Three novel isostructural diphosphates with the general composition Cs3MIIBi(P2O7)2 (MII = Ca, Sr and Pb) and the original framework topology were synthesized and characterized via single-crystal and powder X-ray diffraction, SEM, DTA, and FTIR- and UV-VIS-spectroscopy. In addition, electronic structure (DFT) and Voronoi-Dirichlet polyhedra (VDP) characteristics calculations and crystallochemical analysis were performed...
January 24, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29360833/the-role-of-cgmp-as-a-mediator-of-lipolysis-in-bovine-oocytes-and-its-effects-on-embryo-development-and-cryopreservation
#19
Kátia R L Schwarz, Fernanda C de Castro, Letícia Schefer, Ramon C Botigelli, Daniela M Paschoal, Hugo Fernandes, Cláudia L V Leal
This study aimed to determine the influence of cyclic guanosine 3'5'-monophosphate (cGMP) and cGMP-dependent kinase (PKG) during in vitro maturation (IVM) on lipolysis-related parameters in bovine cumulus-oocyte complexes (COCs), and on embryo development and cryosurvival. COCs were matured with cGMP/PKG modulators and assessed for metaphase II rates (MII), cGMP levels, lipid content in oocytes (OO), transcript abundance for genes involved in lipolysis (ATGL) and lipid droplets (PLIN2) in cumulus cells (CC) and OO, and presence of phosphorylated (active) hormone sensitive lipase (HSLser563) in OO...
2018: PloS One
https://www.readbyqxmd.com/read/29359202/characterization-of-tetraploid-somatic-cell-nuclear-transfer-derived-human-embryonic-stem-cells
#20
Dong-Hyuk Shin, Jeoung-Eun Lee, Jin Hee Eum, Young Gie Chung, Hoon Taek Lee, Dong Ryul Lee
Polyploidy is occurred by the process of endomitosis or cell fusion and usually represent terminally differentiated stage. Their effects on the developmental process were mainly investigated in the amphibian and fishes, and only observed in some rodents as mammalian model. Recently, we have established tetraploidy somatic cell nuclear transfer-derived human embryonic stem cells (SCNT-hESCs) and examined whether it could be available as a research model for the polyploidy cells existed in the human tissues. Two tetraploid hESC lines were artificially acquired by reintroduction of remained 1st polar body during the establishment of SCNT-hESC using MII oocytes obtained from female donors and dermal fibroblasts (DFB) from a 35-year-old adult male...
December 2017: Balsaeng'gwa Saengsig
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