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Kazuki Nosho, Koji Yasuhara, Yuto Ikehata, Tomohiro Mii, Taichiro Ishige, Shunsuke Yajima, Makoto Hidaka, Tetsuhiro Ogawa, Haruhiko Masaki
Most bacterial cells in nature exhibit extremely low colony-forming activity, despite showing various signs of viability, impeding the isolation and utilization of many bacterial resources. However, the general causes responsible for this state of low colony formation are largely unknown. Because liquid cultivation typically yields more bacterial cell cultures than traditional solid cultivation, we hypothesized that colony formation requires one or more specific gene functions that are dispensable or less important for growth in liquid media...
June 15, 2018: Microbiology
Huidan Lou, Lei Yin, Biquan Zhang, Zhong-Wen Ouyang, Bao Li, Zhenxing Wang
By utilizing the quinolinic derivative, 8-carboxymethoxy-2-carboxylicquinoline (L), five transition metal coordination complexes, [M(L)(H2 O)3 ]·H2 O] (M = Mn (1), Co (2)), [Ni(L)(H2 O)2 ] (3), and {[M(L)](H2 O)} n (M = Ni (4), Cu (5)), were synthesized by hydrothermal methods employing similar synthetic strategies. The crystal structures, magnetism and high-field EPR were characterized for the obtained compounds. 1-3 are mononuclear compounds. 1 and 2 have pentagonal bipyramidal geometry, while 4 and 5 exhibit one-dimensional zig-zag chain...
June 15, 2018: Inorganic Chemistry
Long Cui, Li Fang, Xiaodan Mao, Hsun-Ming Chang, Peter C K Leung, Yinghui Ye
Context: Although glial cell line-derived neurotrophic factor (GDNF) and microRNAs (miRNAs) have been shown to regulate mammalian oocyte maturation, little is known about their effects on human oocyte maturation and the underlying molecular mechanisms. Objectives: To examine the effects of GDNF on both nuclear and cytoplasmic maturation in cultured immature human oocytes and to investigate the involvement of miRNAs in GDNF-induced oocyte maturation. Design: A total of 200 human immature oocytes were used to evaluate the effects of GDNF on oocyte maturation...
April 20, 2018: Journal of Clinical Endocrinology and Metabolism
Belén Lledo, Joaquín Llácer, Jose A Ortiz, Beatriz Martinez, Ruth Morales, Rafael Bernabeu
The AR (androgen receptor) polymorphism is associated with POR risk. Furthermore, the use of androgens in POR remains controversial. Our data could clarify the effectiveness of androgen pretreatment. AR genotyping could help us to identify patients at risk for POR and POR patients that will be benefited of androgen pretreatment. OBJECTIVE: The aim of this project was to investigate if the AR (androgen receptor) polymorphism could be used to identify patients at risk for POR and that will benefit from androgens pretreatment...
June 4, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Ans Pauwels, Charlotte Broers, Tim Vanuytsel, Nicolas Pardon, Silvia Cocca, Sabine Roman, Frank Zerbib, Jan Tack, Ricard Farré
Introduction: Approximately 30% of healthy volunteers (HVs) show dilated intercellular spaces in the esophageal epithelium suggesting a functionally reduced epithelial integrity. We aimed to evaluate the presence of an altered epithelial integrity in HVs and whether physiological acid could explain such a difference. Methods: Biopsies for Ussing chamber experiments were taken between 3 cm and 5 cm proximal to the gastroesophageal junction. Twenty-four-hour impedance-pH (MII-pH) monitoring was performed in the same 15 HVs...
May 2018: United European Gastroenterology Journal
L Zakarin Safier, A Gumer, M Kline, D Egli, M V Sauer
PURPOSE: Human oocytes are arguably one of the most important cell types in humans, yet they are one of the least investigated cells. Because oocytes are limited in number, the use of high-quality oocytes is almost entirely in reproduction. Furthermore, regulatory hurdles for research on gametes and regulations on funding related to research on gametes present significant obstacles to research and the advancement of reproductive treatments. Here we report the outcomes of the largest compensated oocyte donation program for research in the USA to date, and probably worldwide...
June 5, 2018: Journal of Assisted Reproduction and Genetics
Lianke Wang, Fabian G Cantú Reinhard, Christian Philouze, Serhiy Demeshko, Sam P de Visser, Franc Meyer, Marcello Gennari, Carole Duboc
Disulfide/thiolate interconversion controlled by Cu is proposed to be involved in relevant biological processes. In analogy to Cu, it can be envisaged that Fe also participates in the control of similar biological processes. We describe herein Fe complexes that undergo FeIII-thiolate/FeII-disulfide (inter)conversion mediated by halide (de)coordination, and by the nature of the solvent. The dinuclear FeII-disulfide complex [FeII2(LSSL)]2+ ((LS)2- = 2,2'-(2,2'-bipyridine-6,6'-diyl)bis(1,1-diphenylethanethiolate), (LSSL)2-, the corresponding disulfide ligand) shows solvent-dependent properties...
June 5, 2018: Chemistry: a European Journal
Mehdi Mohsenzadeh, Amin Salehi-Abargouei, Nasim Tabibnejad, Mojgan Karimi-Zarchi, Mohammad Ali Khalili
OBJECTIVE: There are controversies regarding in vitro maturation (IVM) procedure, the time of storing frozen oocytes and maturation stage of vitrified oocytes and its impact on oocytes fertilization capability. The aim of this systematic review and meta-analysis was to evaluate the impact of vitrification on human oocytes during IVM procedure. STUDY DESIGN: A systematic review with meta-analysis was undertaken. Main search terms were those related key words. We searched Medline, Embase, Scopus and ISI web of science to detect English-language studies...
May 21, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Sanghoon Lee, Jun-Xue Jin, Anukul Taweechaipaisankul, Geon-A Kim, Byeong-Chun Lee
Melatonin is a multifunctional molecule with numerous biological activities. The fact that melatonin modulates the functions of porcine granulosa cells via the MT2 receptor suggests the possibility of MT2 receptor-mediation for melatonin to promote cumulus expansion of porcine cumulus-oocyte complexes (COCs). Therefore, we investigated the presence of MT2 in porcine COCs, and the effects of melatonin with or without selective MT2 antagonists (luzindole and 4-P-PDOT) on this process; COCs underwent in vitro maturation culturing with six different conditions (control, melatonin, luzindole, 4-P-PDOT, melatonin + luzindole or melatonin + 4-P-PDOT)...
May 26, 2018: International Journal of Molecular Sciences
Maierhaba Abudoureheman, Xiaobo Pan, Shujuan Han, Yilimiranmu Rouzhahong, Zhihua Yang, Hongping Wu, Shilie Pan
By the application of cation substitution, five new members of cyclophosphates, RbCdP3 O9 , CsPbP3 O9 , CsSrP3 O9 , RbMgP3 O9 , and RbCaP3 O9 , were obtained by a high-temperature melt method and structurally analyzed. The five compounds have identical stoichiometries, and all of them feature a three-dimensional network, which consists of MII O6 (MII = Cd, Mg, Ca, Pb, Sr) octahedra and cyclic P3 O9 units, while alkali-metal atoms are located within the network. However, RbCdP3 O9 and CsPbP3 O9 belong to the asymmetric space groups P6̅ c2 and Pna21 , respectively, and the other three compounds belong to orthorhombic space group Pnma...
May 30, 2018: Inorganic Chemistry
Nassim Ghorbanmehr, Mojdeh Salehnia, Mahboobeh Amooshahi
Objective: The aim of present study is to determine the effects of supplementation of oocyte maturation medium with sodium selenite (SS) on oocyte mitochondrial DNA (mtDNA) copy number and reactive oxygen species (ROS) levels. Materials and Methods: In this experimental study, germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) stage oocytes were recovered from 6-8 week old female mice after superovulation. Some of the GV oocytes were cultured and matured in the presence and absence of SS...
October 2018: Cell Journal
Nikolai Jaschke, Fabian Lunger, Ludwig Wildt, Beata Seeber
PURPOSE: To compare the concentrations of beta endorphin in serum and follicular fluid (FF) of PCOS- and non-PCOS women. Secondarily, to investigate associations between beta endorphin and other parameters. METHODS: Fifty-nine women undergoing in vitro fertilization (IVF) were included in the study. Sixteen were stratified to the PCOS group using the Rotterdam criteria. The remaining 43 women served as controls. Follicular fluid was collected during oocyte retrieval and peripheral blood sampling was performed on the same day...
May 28, 2018: Archives of Gynecology and Obstetrics
Amir Arav, Yehudit Natan, Dorit Kalo, Alisa Komsky-Elbaz, Zvika Roth, Paolo Emanuele Levi-Setti, Milton Leong, Pasquale Patrizio
PURPOSE: This paper reports the use of a novel automatic vitrification device (Sarah, Fertilesafe, Israel) for cryopreservation of oocytes and embryos. METHODS: Mice oocytes (n = 40) and embryos (8 cells, n = 35 and blastocysts, n = 165), bovine embryos (2PN, n = 35), and MII oocytes (n = 84) were vitrified using this automated device. A total of 42 (2 cells) mice embryos, 20 (2PN) bovine embryos, and 150 MII bovine oocytes were used as fresh controls and grown to blastocysts...
May 25, 2018: Journal of Assisted Reproduction and Genetics
De-Jie Chen, Fen-Fei Gao, Xiao-Kuang Ma, Gang-Gang Shi, Yuan-Bing Huang, Quang-Xi Su, Sterling Sudweeks, Ming Gao, Turner Dharshaun, Jason Brek Eaton, Yong-Chang Chang, J Michael Mcintosh, Ronald J Lukas, Paul Whiteaker, Scott C Steffensen, Jie Wu
Neuronal nicotinic acetylcholine receptors containing α6 subunits (α6* -nAChRs) show highly restricted distribution in midbrain neurons associated with pleasure, reward, and mood control, suggesting an important impact of α6* -nAChRs in modulating mesolimbic functions. However, the function and pharmacology of α6* -nAChRs remain poorly understood because of the lack of selective agonists for α6* -nAChRs and the challenging heterologous expression of functional α6* -nAChRs in mammalian cell lines. In particular, the α6 subunit is commonly co-expressed with α4* -nAChRs in the midbrain, which masks α6* -nAChR (without α4) function and pharmacology...
May 24, 2018: Acta Pharmacologica Sinica
D C Steinemann, P C Müller, A-C Schwarz, A Zerz, G R Linke, B P Müller-Stich
Laparoscopic transgastric stapler-assisted mucosectomy (SAM) has been described for minimally invasive circumferential en bloc resection of Barrett's esophagus (BE). Conceivably long-term disease control might be achieved by adding antireflux surgery after resection of BE by SAM. The aim of this study was to assess the feasibility of combined SAM and fundoplication in one laparoscopic procedure in six pigs. Furthermore, the competence of the gastroesophageal junction (GEJ) was assessed at baseline, after SAM, and after subsequent laparoscopic fundoplication...
May 21, 2018: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
Beatriz Rioseras, Pavel V Sliaha, Vladimir Gorshkov, Paula Yagüe, Maria T López-García, Nathaly González-Quiñónez, Sergey Kovalchuk, Adelina Rogowska-Wrzesinska, Ole N Jensen, Angel Manteca
Streptomycetes are multicellular bacteria with complex developmental cycles. They are of biotechnological importance as they produce most bioactive compounds used in biomedicine, e.g. antibiotic, antitumoral and immunosupressor compounds. Streptomyces genomes encode a large number of Ser/Thr/Tyr kinases, making this genus an outstanding model for the study of bacterial protein phosphorylation events. We used mass spectrometry based quantitative proteomics and phosphoproteomics to characterize bacterial differentiation and activation of secondary metabolism of Streptomyces coelicolor...
May 21, 2018: Molecular & Cellular Proteomics: MCP
Bo Pan, Julang Li
Cumulus cells and the remodeling of their extracellular matrix around oocytes are essential for oocyte maturation and ovulation in the ovary. An important extracellular metalloprotease, ADAMTS1, and its partner VERSICAN, mediate essential cumulus-oocyte-complex (COC) structural remodeling. However, how the expression of these proteinases is regulated during oocyte maturation is unclear. Here we report that both ADAMTS1 and VERSICAN significantly increased in porcine cumulus cells during COC in vitro maturation (IVM)...
May 15, 2018: Molecular and Cellular Endocrinology
Shinji Mii, Akiyoshi Hoshino, Atsushi Enomoto, Yoshiki Murakumo, Masako Ito, Akira Yamaguchi, Masahide Takahashi
Osteoporosis is a global public health problem that is increasing along with an aging population. A major determinant of osteoporosis is high bone turnover, which results from osteoclast activation. CD109 is a glycosylphosphatidylinositol-anchored glycoprotein, a deficiency that leads to a psoriasis-like skin inflammation in mice. Although the expression of CD109 has been reported in mouse pre-osteoclast cells, its function in osteoclasts in vivo remains largely unknown. To investigate the physiological role of CD109 in bone metabolism, we analyzed bones from wild-type and CD109-deficient adult mice...
May 16, 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Yu-Feng Song, Xiao-Ying Tan, Ya-Xiong Pan, Li-Han Zhang, Qi-Liang Chen
Although several studies have been conducted to study leptin function, information is very scarce on the molecular mechanism of leptin in fatty acid β-oxidation and oocytes maturation in fish. In this study, we investigated the potential role of fatty acid β-oxidation in leptin-mediated oocytes maturation in Pelteobagrus fulvidraco . Exp. 1 investigated the transcriptomic profiles of ovary and the differential expression of genes involved in β-oxidation and oocytes maturation following rt-hLEP injection; rt-hLEP injection was associated with significant changes in the expression of genes, including twenty-five up-regulated genes ( CPT1 , Acsl , Acadl , Acadm , Hadhb , Echsl , Hsd17b4 , Acca , PPARα , CYP8B1 , ACOX1 , ACBP , MAPK , RINGO , Cdc2 , MEK1 , IGF-1R , APC/C, Cdk2 , GnRHR, STAG3 , SMC1 , FSHβ and C-Myc ) and ten down-regulated gene ( PPARγ , FATCD36 , UBC , PDK1 , Acads , Raf , Fizzy , C3H-4 , Raf and PKC ), involved in fatty acid β-oxidation and oocytes maturation...
May 14, 2018: International Journal of Molecular Sciences
C Zhong, K Liu, K Wang, H Liu, H Su, J Wu, L Duan
Patients with gastroesophageal reflux disease (GERD) can present with typical or atypical symptoms. The aim of this study is to explore the underlying physiological and psychological mechanisms that lead to different symptomatic manifestations of GERD. A total of 238 patients diagnosed with GERD underwent gastroscopy, 24 h multichannel intraluminal impedance-pH (MII-pH) monitoring, and psychological assessment with questionnaires. Patient symptoms were used to classify GERD into phenotypes of typical reflux syndrome (TRS, n = 87), reflux chest pain syndrome (RCS, n = 98), and extraesophageal syndromes (EES, n = 53)...
May 10, 2018: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
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