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https://www.readbyqxmd.com/read/28545260/thermostabilization-of-the-uronate-dehydrogenase-from-agrobacterium-tumefaciens-by-semi-rational-design
#1
Teresa Roth, Barbara Beer, André Pick, Volker Sieber
Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natural resources such as seaweed or pectin as raw material. These contain large amounts of uronic acids as major constituents such as glucuronic acid and galacturonic acid which can be converted into the corresponding aldaric acids via an enzyme-based oxidation using uronate dehydrogenase (Udh)...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28544657/a-strategy-to-enhance-the-efficiency-of-quantum-dot-sensitized-solar-cells-by-decreasing-the-electron-recombinations-with-polyoxometalate-tio2-as-the-electronic-interface-layer
#2
Li Chen, Wei-Lin Chen, Jian-Ping Li, Jia-Bo Wang, En-Bo Wang
The serious electron recombination occurring in TiO2/quantum dot sensitizer/electrolyte interface is the key reason for hindering the further efficiency improvement of QDSCs. Polyoxometalate(POM) can be as electron transfer media to decreasing electron recombination of photoelectric device, which is due to the excellent oxidation reduction properties and thermostability. So in this paper, POM/TiO2 electronic interface layer prepared by a simple layer-by-layer self-assembly method is added between FTO and mesoporous TiO2 in the photoanode of the QDSCs, and their effects on the photovoltaic performance are systematically investigated...
May 23, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28544154/floral-nectar-of-the-obligate-outcrossing-canavalia-gladiata-jacq-dc-fabaceae-contains-only-one-predominant-protein-a-class-iii-acidic-chitinase
#3
Xue-Long Ma, Richard I Milne, Hong-Xia Zhou, Jiang-Yu Fang, Hong-Guang Zha
Floral nectar can affect the fitness of insect-pollinated plants, through both attraction and manipulation of pollinators. Self-incompatible insect-pollinated plants receive more insect visits than their self-compatible relatives, and the nectar of such species might face more risk to be infested by the pathogens carried by pollinators than self-compatible plants. Proteins in nectar (nectarins) play an important role in the protection of nectar, but little is known regarding nectarins in self-incompatible species...
May 20, 2017: Plant Biology
https://www.readbyqxmd.com/read/28543753/%C3%AE-rep-a3-a-versatile-artificial-scaffold-for-metalloenzyme-design
#4
Thibault Di Meo, Wadih Ghattas, Christian Herrero, Christophe Velours, Philippe Minard, Jean-Pierre Mahy, Rémy Ricoux, Agathe Urvoas
αRep is a new family of artificial proteins based on a thermostable alpha-helical repeated motif. One of its members, αRep A3, forms a stable homo-dimer with a wide cleft that is able to receive metal complexes and thus appears as suitable for generating new artificial biocatalysts. Based on the crystal structure of αRep A3, two positions (F119 and Y26) were chosen and changed independently into cysteine residues. A phenanthroline ligand was covalently attached to the unique cysteine of each protein variant and the corresponding biohybrids were purified and characterized...
May 22, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28541146/assessment-of-the-immunogenicity-of-rabies-vaccine-preserved-by-vaporization-and-delivered-to-the-duodenal-mucosa-of-gray-foxes-urocyon-cinereoargenteus
#5
Todd G Smith, Xianfu Wu, James A Ellison, Ashutosh Wadhwa, Richard Franka, Gregory L Langham, Brianna L Skinner, Cathleen A Hanlon, Victor L Bronshtein
OBJECTIVE To assess the immunogenicity of thermostable live-attenuated rabies virus (RABV) preserved by vaporization (PBV) and delivered to the duodenal mucosa of a wildlife species targeted for an oral vaccination program. ANIMALS 8 gray foxes (Urocyon cinereoargenteus). PROCEDURES Endoscopy was used to place RABV PBV (n = 3 foxes), alginate-encapsulated RABV PBV (3 foxes), or nonpreserved RABV (2 foxes) vaccine into the duodenum of foxes. Blood samples were collected weekly to monitor the immune response...
June 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28540682/the-limitations-of-existing-approaches-in-improving-microrna-target-prediction-accuracy
#6
Rasiah Loganantharaj, Thomas A Randall
MicroRNAs (miRNAs) are small (18-24 nt) endogenous RNAs found across diverse phyla involved in posttranscriptional regulation, primarily downregulation of mRNAs. Experimentally determining miRNA-mRNA interactions can be expensive and time-consuming, making the accurate computational prediction of miRNA targets a high priority. Since miRNA-mRNA base pairing in mammals is not perfectly complementary and only a fraction of the identified motifs are real binding sites, accurately predicting miRNA targets remains challenging...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28533982/lid-opening-and-conformational-stability-of-t1-lipase-is-mediated-by-increasing-chain-length-polar-solvents
#7
Jonathan Maiangwa, Mohd Shukuri Mohamad Ali, Abu Bakar Salleh, Raja Noor Zaliha Raja Abd Rahman, Yahaya M Normi, Fairolniza Mohd Shariff, Thean Chor Leow
The dynamics and conformational landscape of proteins in organic solvents are events of potential interest in nonaqueous process catalysis. Conformational changes, folding transitions, and stability often correspond to structural rearrangements that alter contacts between solvent molecules and amino acid residues. However, in nonaqueous enzymology, organic solvents limit stability and further application of proteins. In the present study, molecular dynamics (MD) of a thermostable Geobacillus zalihae T1 lipase was performed in different chain length polar organic solvents (methanol, ethanol, propanol, butanol, and pentanol) and water mixture systems to a concentration of 50%...
2017: PeerJ
https://www.readbyqxmd.com/read/28533178/mucin-and-carbohydrate-stimulated-adhesion-and-subproteome-changes-of-the-probiotic-bacterium-lactobacillus-acidophilus-ncfm
#8
Hasan Ufuk Celebioglu, Sita Vaag Olesen, Kennie Prehn, Sampo J Lahtinen, Susanne Brix, Maher Abou Hachem, Birte Svensson
Adhesion to intestinal mucosa is a crucial property for probiotic bacteria. Adhesion is thought to increase host-bacterial interactions, thus potentially enabling health benefits to the host. Molecular events connected with adhesion and surface proteome changes were investigated for the probiotic Lactobacillus acidophilus NCFM cultured with established or emerging prebiotic carbohydrates as carbon source and in the presence of mucin, the glycoprotein of the epithelial mucus layer. Variation in adhesion to HT29-cells and mucin was associated with carbon source and mucin-induced subproteome abundancy differences...
May 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28530596/phytochemicals-content-and-antioxidant-properties-of-sea-buckthorn-hippophae-rhamnoides-l-as-affected-by-heat-treatment-quantitative-spectroscopic-and-kinetic-approaches
#9
Florentina-Mihaela Ursache, Ioana Otilia Ghinea, Mihaela Turturică, Iuliana Aprodu, Gabriela Râpeanu, Nicoleta Stănciuc
The effect of thermal processing (50-100°C) on the degradation of the phytochemicals in sea buckthorn extract was investigated using chromatographic, fluorescence and FT-IR spectroscopy techniques and degradation kinetics. Heating the sea buckthorn extract resulted in structural changes that led to red- or blue-shifts in maximum emission, depending on temperature and excitation wavelengths. The attenuated total reflectance analysis of the sea buckthorn extract revealed a satisfactory thermostability of compounds at high temperatures...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28528790/characterization-of-a-novel-thermostable-gh45-endoglucanase-from-chaetomium-thermophilum-and-its-biodegradation-of-pectin
#10
Qinzheng Zhou, Peng Ji, Jianye Zhang, Xue Li, Chao Han
A novel thermostable endoglucanase (CTendo45) encoding gene was cloned from Chaetomium thermophilum and heterologously expressed in Pichia pastoris. Sequence alignment indicated that the CTendo45 enzyme belonged to glycoside hydrolase family 45. The recombinant enzyme was purified by Ni(2+) affinity chromatography, and its apparent molecular mass was estimated to be 32 kDa by SDS-PAGE. The purified enzyme displayed maximum activity at 70°C and pH 4. CTendo45 was stable at 60°C for 1 h, and residual activities of 78...
May 19, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28525726/characterization-of-an-ionic-liquid-tolerant-%C3%AE-xylosidase-from-a-marine-derived-fungal-endophyte
#11
Anindita Sengupta, Angela Zabala, Si Yu Tan, Arthur Broadstock, T S Suryanarayanan, Venkat Gopalan
Ionic liquids (ILs) are used in lignocellulosic biomass (LCB) pretreatment due to their ability to disrupt the extensive hydrogen-bonding network in cellulose and hemicellulose, and thereby decrease LCB recalcitrance to subsequent enzymatic degradation. However, this approach necessitates development of a cellulases and hemicellulases that can tolerate ~20% (w/v) IL, an amount that either co-precipitates with the sugar polymers after the initial pretreatment or is typically used in single-pot biomass deconstructions...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28520091/selective-analysis-of-aristolochic-acid-i-in-herbal-medicines-by-dummy-molecularly-imprinted-solid-phase-extraction-and-hplc
#12
Yanhui Ge, Pengqi Guo, Xinya Xu, Guoning Chen, Xuemei Zhang, Hua Shu, Bilin Zhang, Zhimin Luo, Chun Chang, Qiang Fu
In this study, surface molecularly imprinted polymers were prepared as the selective sorbents for separation of aristolochic acid I in herbal medicine extracts by a facile approach. A less toxic dummy-template, ofloxacin, was used to create specific molecule recognition sites for aristolochic acid I in the synthesized polymers. The polymers were characterized by Fourier-transfer infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, elemental analysis and nitrogen adsorption-desorption test...
May 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28515785/recombinant-expression-of-thermostable-processive-mteg5-endoglucanase-and-its-synergism-with-mtlpmo-from-myceliophthora-thermophila-during-the-hydrolysis-of-lignocellulosic-substrates
#13
Anthi Karnaouri, Madhu Nair Muraleedharan, Maria Dimarogona, Evangelos Topakas, Ulrika Rova, Mats Sandgren, Paul Christakopoulos
BACKGROUND: Filamentous fungi are among the most powerful cellulolytic organisms in terrestrial ecosystems. To perform the degradation of lignocellulosic substrates, these microorganisms employ both hydrolytic and oxidative mechanisms that involve the secretion and synergism of a wide variety of enzymes. Interactions between these enzymes occur on the level of saccharification, i.e., the release of neutral and oxidized products, but sometimes also reflected in the substrate liquefaction...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28515315/the-c-terminal-peptide-of-aquifex-aeolicus-riboflavin-synthase-directs-encapsulation-of-native-and-foreign-guests-by-a-cage-forming-lumazine-synthase
#14
Yusuke Azuma, Reinhard Zschoche, Donald Hilvert
Encapsulation of specific enzymes in self-assembling protein cages is a hallmark of bacterial compartments that function as counterparts to eukaryotic organelles. The cage-forming enzyme lumazine synthase (LS) from Bacillus subtilis (BsLS), for example, encapsulates riboflavin synthase (BsRS), enabling channeling of lumazine from the site of its generation to the site of its conversion to vitamin B2. Elucidating the molecular mechanisms underlying the assembly of these supramolecular complexes could help inform new approaches for metabolic engineering, nanotechnology, and drug delivery...
May 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28512177/evaluation-of-novel-process-indicators-for-rapid-monitoring-of-hydrogen-peroxide-decontamination-processes
#15
Neil P McLeod, Melanie Clifford, J Mark Sutton
Geobacillus stearothermophilus spores on stainless steel discs are routinely used as Biological Indicators (BIs) for the validation of hydrogen peroxide bio-decontamination processes. Given ongoing concerns about the reliability and response time of BIs, we explored the potential for an enzyme-based approach decontamination process evaluation. Thermostable adenylate kinase (tAK) enzyme was coated onto a solid support and exposed to hydrogen peroxide vapour, in parallel with standard commercial 6-log BIs, during a series of vapour-phase hydrogen peroxide (VPHP) cycles in a flexible film isolator...
May 15, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28508385/improving-the-temperature-characteristics-and-catalytic-efficiency-of-a-mesophilic-xylanase-from-aspergillus-oryzae-aoxyn11a-by-iterative-mutagenesis-based-on-in-silico-design
#16
Xue-Qing Li, Qin Wu, Die Hu, Rui Wang, Yan Liu, Min-Chen Wu, Jian-Fang Li
To improve the temperature characteristics and catalytic efficiency of a glycoside hydrolase family (GHF) 11 xylanase from Aspergillus oryzae (AoXyn11A), its variants were predicted based on in silico design. Firstly, Gly(21) with the maximum B-factor value, which was confirmed by molecular dynamics (MD) simulation on the three-dimensional structure of AoXyn11A, was subjected to site-saturation mutagenesis. Thus, one variant with the highest thermostability, AoXyn11A(G21I), was selected from the mutagenesis library, E...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28504167/antibacterial-and-antioxidative-activity-of-o-amine-functionalized-chitosan
#17
Tamer M Tamer, Mohamed A Hassan, Ahmed M Omer, Katarína Valachová, Mohamed S Mohy Eldin, Maurice N Collins, Ladislav Šoltés
Cinnamaldehyde was immobilized to O-amine functionalized chitosan via a coupling reaction. Fourier transform infrared spectroscopy confirmed N-cinnamyl substitution. Wetting analyses demonstrate more hydrophobicity in the N-cinnamyl substituted O-amine functionalized chitosan compared to chitosan or unsubstituted O-amine functionalized chitosan. Thermal gravimetric analysis and differential scanning calorimetry demonstrates that the prepared N-cinnamyl substituted O-amine functionalized chitosan exhibits higher thermostability than unmodified chitosan at temperatures in which polysaccharides are commonly stored and utilised...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28503195/strategies-for-improved-isopropanol-butanol-production-by-a-clostridium-strain-from-glucose-and-hemicellulose-through-consolidated-bioprocessing
#18
Fengxue Xin, Tianpeng Chen, Yujiang Jiang, Weiliang Dong, Wenming Zhang, Min Zhang, Hao Wu, Jiangfeng Ma, Min Jiang
BACKGROUND: High cost of traditional substrates and formation of by-products (such as acetone and ethanol) in acetone-butanol-ethanol (ABE) fermentation hindered the large-scale production of biobutanol. Here, we comprehensively characterized a newly isolated solventogenic and xylanolytic Clostridium species, which could produce butanol at a high ratio with elimination of ethanol and conversion of acetone to more value-added product, isopropanol. Ultimately, direct butanol production from hemicellulose was achieved with efficient expression of indigenous xylanase by the novel strain via consolidated bioprocessing...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28501639/comparative-evaluation-of-thermal-decomposition-behavior-and-thermal-stability-of-powdered-ammonium-nitrate-under-different-atmosphere-conditions
#19
Man Yang, Xianfeng Chen, Yujie Wang, Bihe Yuan, Yi Niu, Ying Zhang, Ruoyu Liao, Zumin Zhang
In order to analyze the thermal decomposition characteristics of ammonium nitrate (AN), its thermal behavior and stability under different conditions are studied, including different atmospheres, heating rates and gas flow rates. The evolved decomposition gases of AN in air and nitrogen are analyzed with a quadrupole mass spectrometer. Thermal stability of AN at different heating rates and gas flow rates are studied by differential scanning calorimetry, thermogravimetric analysis, paired comparison method and safety parameter evaluation...
April 28, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28500928/molecular-cloning-characterization-and-in-silico-analysis-of-a-thermostable-%C3%AE-glucosidase-enzyme-from-putranjiva-roxburghii-with-a-significant-activity-for-cellobiose
#20
Bibekananda Kar, Preeti Verma, Girijesh Kumar Patel, Ashwani Kumar Sharma
The native Putranjiva roxburghii family 1 glycoside hydrolase enzyme showed β-D-fucosidase activity in addition to β-D-glucosidase and β-D-galactosidase activities reported in our previous study. A single step concanvalin A affinity chromatography for native PRGH1 improved the yield and reduced the purification time. The PRGH1 gene was cloned and overexpressed in E. coli. The full length gene contained an ORF of 1617 bp encoding a polypeptide of 538 amino acids. The amino acid sequence of PRGH1 showed maximum similarities to β-glucosidases and myrosinases...
May 10, 2017: Phytochemistry
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