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https://www.readbyqxmd.com/read/28102511/some-nutritional-characteristics-of-enzymatically-resistant-maltodextrin-from-cassava-manihot-esculenta-crantz-starch
#1
Rocío Toraya-Avilés, Maira Segura-Campos, Luis Chel-Guerrero, David Betancur-Ancona
Cassava (Manihot esculenta Crantz) native starch was treated with pyroconversion and enzymatic hydrolysis to produce a pyrodextrin and an enzyme-resistant maltodextrin. Some nutritional characteristics were quantified for both compounds. Pyroconversion was done using a 160:1 (p/v) starch:HCl ratio, 90 °C temperature and 3 h reaction time. The resulting pyrodextrin contained 46.21% indigestible starch and 78.86% dietary fiber. Thermostable α-amylase (0.01%) was used to hydrolyze the pyrodextrin at 95 °C for 5 min...
January 19, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28102398/low-temperature-heat-capacity-standard-entropy-standard-enthalpy-and-magnetic-property-a-new-1d-cu-ii-coordination-polymer-incorporating-tetrazole-1-acetic-acid-and-p-nitrobenzoic-acid
#2
Qi Yang, Jing Ge, Xin Liu, Quan Shi, Hongshan Ke, Qing Wei, Gang Xie, Sanping Chen, Shengli Gao
A new 1D Cu(II) coordination polymer, formulated as {[Cu(TZA)(PNA)]·H2O}n (1) (HTZA = tetrazole-1-acetic acid, HPNA = p-nitrobenzoic acid), was synthesized and structurally characterized. Thermogravimetric analysis demonstrated that the main frame of 1 exhibited good thermostability up to 473 K. The non-isothermal kinetics for the first exothermic process of 1 were studied by Kissinger and Ozawa methods. The magnetic study revealed that 1 possessed antiferromagnetic exchange interactions between Cu(II) ions through the carboxyl-bridge...
January 19, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28101787/a-recombinant-highly-thermostable-%C3%AE-mannanase-retman26-from-thermophilic-bacillus-subtilis-tbs2-expressed-in-pichia-pastoris-and-its-ph-and-temperature-stability
#3
Zhangcai Luo, Jing Miao, Guoying Li, Yao Du, Xiaobin Yu
A gene encoding a highly thermostable β-mannanase from a thermophilic Bacillus subtilis (TBS2) was successfully expressed in Pichia pastoris. The maximum activity of the recombinant thermostable β-mannanase (ReTMan26) was 5435 U/mL, which was obtained by high-density, fed-batch cultivation after 168-h induction with methanol in a 50-L bioreactor. The protein yield reached 3.29 mg/mL, and the protein had a molecular weight of ~42 kDa. After fermentation, ReTMan26 was purified using a 10-kDa cut-off membrane and Sephadex G-75 column...
January 18, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28100651/stabilization-of-bacillus-circulans-xylanase-by-combinatorial-insertional-fusion-to-a-thermophilic-host-protein
#4
Vandan Shah, Brennal Pierre, Tamari Kirtadze, Seung Shin, Jin Ryoun Kim
High thermostability of an enzyme is critical for its industrial application. While many engineering approaches such as mutagenesis have enhanced enzyme thermostability, they often suffer from reduced enzymatic activity. A thermally stabilized enzyme with unchanged amino acids is preferable for subsequent functional evolution necessary to address other important industrial needs. In the research presented here, we applied insertional fusion to a thermophilic maltodextrin-binding protein from Pyrococcus furiosus (PfMBP) in order to improve the thermal stability of Bacillus circulans xylanase (BCX)...
January 17, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28098812/novel-aflatoxin-degrading-enzyme-from-bacillus-shackletonii-l7
#5
Liang Xu, Mohamed Farah Eisa Ahmed, Lancine Sangare, Yueju Zhao, Jonathan Nimal Selvaraj, Fuguo Xing, Yan Wang, Hongping Yang, Yang Liu
Food and feed contamination by aflatoxin (AF)B₁ has adverse economic and health consequences. AFB₁ degradation by microorganisms or microbial enzymes provides a promising preventive measure. To this end, the present study tested 43 bacterial isolates collected from maize, rice, and soil samples for AFB₁-reducing activity. The higher activity was detected in isolate L7, which was identified as Bacillus shackletonii. L7 reduced AFB₁, AFB₂, and AFM₁ levels by 92.1%, 84.1%, and 90.4%, respectively, after 72 h at 37 °C...
January 14, 2017: Toxins
https://www.readbyqxmd.com/read/28097910/purification-and-biochemical-characterization-of-a-novel-thermostable-serine-alkaline-protease-from-aeribacillus-pallidus-c10-a-potential-additive-for-detergents
#6
Vildan Yildirim, Mustafa Ozkan Baltaci, Ilknur Ozgencli, Melda Sisecioglu, Ahmet Adiguzel, Gulsah Adiguzel
An extracellular thermostable alkaline serine protease enzyme from Aeribacillus pallidus C10 (GenBank No: KC333049), was purified 4.85 and 17. 32-fold with a yield of 26.9 and 19.56%, respectively, through DE52 anion exchange and Probond affinity chromatography. The molecular mass of the enzyme was determined through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with approximately 38.35 kDa. The enzyme exhibited optimum activity at pH 9 and at temperature 60 °C. It was determined that the enzyme had remained stable at the range of pH 7...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28097168/purification-and-biochemical-properties-of-a-thermostable-haloalkaline-cellulase-from-bacillus-licheniformis-amf-07-and-its-application-for-hydrolysis-of-different-cellulosic-substrates-to-bioethanol-production
#7
Fatemeh Azadian, Arastoo Badoei-Dalfard, Abdolhamid Namaki-Shoushtari, Mehdi Hassanshahian
A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa. The CMCase enzyme was highly active and stable over broad ranges of temperature (40-80ºC), pH (6...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28093975/molecular-modeling-and-dynamics-simulation-analysis-of-katnal1-for-identification-of-novel-inhibitor-of-sperm-maturation
#8
Kishore Sarma, Shubhadeep Roychoudhury, Sudipta Sankar Bora, Budheswar Dehury, Pratap Parida, Saurav Das, Robin Das, Chandrajit Dohutia, Sangeeta Nath, Bibhas Deb, Mahendra K Modi
Rapid growth of world population is one of the biggest challenges faced by the human race. Hormone based birth control often causes various side effects, as such development of non-hormonal based contraceptives is the need of the hour. A recent study revealed that temporary infertility without changing hormone levels can be attained by inhibiting Katanin p60 ATPase-containing subunit A-like 1 protein (KATNAL1) which is critical in sperm maturation in the testes. This study revealed that, KATNAL1 is a soluble protein retained in the cytoplasm having molecular weight of 55,392 Dalton...
January 16, 2017: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/28089379/characterization-of-a-novel-dyp-type-peroxidase-from-streptomyces-avermitilis
#9
Kanako Sugawara, Yuriko Nishihashi, Tomomi Narioka, Toru Yoshida, Mifumi Morita, Yasushi Sugano
DyP-type peroxidases are a heme peroxidase family with unique properties whose members are widely distributed from prokaryotes to eukaryotes. DyP-type peroxidases are subdivided into class P, I and V based on structure-based sequence alignment. Class V enzymes possess degradation activities for anthraquinone dyes, and include extra sequences compared with class P and I. Class V enzymes are mainly found in fungi, with only two such proteins, AnaPX and DyP2, reported in bacteria. Here, we heterologously expressed, purified and biochemically characterized SaDyP2 protein, predicted to belong to class V...
January 11, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28087769/enzyme-efficiency-but-not-thermostability-drives-cefotaxime-resistance-evolution-in-tem-1-%C3%AE-lactamase
#10
Jennifer Knies, Fei Cai, Daniel M Weinreich
A leading intellectual challenge in evolutionary genetics is to identify the specific phenotypes that drive adaptation. Enzymes offer a particularly promising opportunity to pursue this question, because many enzymes' contributions to organismal fitness depend on a comparatively small number of experimentally accessible properties. Moreover, on first principles the demands of enzyme thermostability stand in opposition to the demands of catalytic activity. This observation, coupled with the fact that enzymes are only marginally thermostable, motivates the widely held hypothesis that mutations conferring functional improvement require compensatory mutations to restore thermostability...
January 12, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28087448/fabrication-and-characterization-of-electrospun-cellulose-nano-hydroxyapatite-nanofibers-for-bone-tissue-engineering
#11
Chenghong Ao, Yan Niu, Ximu Zhang, Xu He, Wei Zhang, Canhui Lu
Nanofibrous scaffolds from cotton cellulose and nano-hydroxyapatite (nano-HA) were electrospun for bone tissue engineering. The solution properties of cellulose/nano-HA spinning dopes and their associated electrospinnability were characterized. Morphological, thermal and mechanical properties of the electrospun cellulose/nano-HA nanocomposite nanofibers (ECHNN) were measured and the biocompatibility of ECHNN with human dental follicle cells (HDFCs) was evaluated. Scanning electron microscope (SEM) images indicated that the average diameter of ECHNN increased with a higher nano-HA loading and the fiber diameter distributions were well within the range of natural ECM (extra cellular matrix) fibers (50-500nm)...
January 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28077575/effector-attenuating-substitutions-that-maintain-antibody-stability-and-reduce-toxicity-in-mice
#12
Megan Lo, Hok Seon Kim, Raymond K Tong, Travis W Bainbridge, Jean-Michel Vernes, Yin Zhang, Yuwen Linda Lin, Shan Chung, Mark S Dennis, Y Joy Yu Zuchero, Ryan J Watts, Jessica A Couch, Y Gloria Meng, Jasvinder K Atwal, Randall J Brezski, Christoph Spiess, James A Ernst
The antibody Fc region regulates antibody cytotoxic activities and serum half-life. In a therapeutic context however, the cytotoxic effector function of an antibody is often not desirable and can create safety liabilities by activating native host immune defenses against cells expressing the receptor-antigens. Several amino acid changes in the Fc region have been reported to silence or reduce the effector function of antibodies. These earlier studies focused primarily on the interaction of human antibodies with human Fc-Gamma; receptors, and it remains largely unknown how such changes to the Fc might translate in the context of a murine antibody...
January 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28075353/biochemical-and-structural-insights-into-a-novel-thermostable-%C3%AE-1-3-galactosidase-from-marinomonas-sp-bsi20414
#13
Haitao Ding, Qian Zeng, Lili Zhou, Yong Yu, Bo Chen
A novel β-1,3-galactosidase, designated as MaBGA (β-galactosidase from Marinomonas sp. BSi20414), was successfully purified to homogeneity from Marinomonas sp. BSi20414 isolated from Arctic sea ice by ammonium sulfate precipitation and anion exchange chromatography, resulting in an 8.12-fold increase in specific activity and 9.9% recovery in total activity. MaBGA displayed its maximum activity at pH 6.0 and 60 °C, and maintained at least 90% of its initial activity over the pH range of 5.0-8.0 after incubating for 1 h...
January 8, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28075069/ebola-vaccination-using-a-dna-vaccine-coated-on-plga-pll-%C3%AE-pga-nanoparticles-administered-using-a-microneedle-patch
#14
Hung-Wei Yang, Ling Ye, Xin Dong Guo, Chinglai Yang, Richard W Compans, Mark R Prausnitz
Ebola DNA vaccine is incorporated into PLGA-PLL/γPGA nanoparticles and administered to skin using a microneedle (MN) patch. The nanoparticle delivery system increases vaccine thermostability and immunogenicity compared to free vaccine. Vaccination by MN patch produces stronger immune responses than intramuscular administration.
January 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28074318/boosted-large-scale-production-and-purification-of-a-thermostable-archaeal-phosphotriesterase-like-lactonase-for-organophosphate-decontamination
#15
Odile Francesca Restaino, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Elena Porzio, Giuseppe Manco, Luigi Fedele, Cinzia Donatiello, Mario De Rosa, Chiara Schiraldi
Thermostable phosphotriesterase-like lactonases (PLLs) from extremophile archaea, like SsoPox from Sulfolobus solfataricus, are attractive biotechnological tools with industrial applications as organophosphate decontaminants, but their manufacturing still remains an unresolved issue because of the high costs and the low production yields. In this paper, for the first time, an efficient biotechnological process for the production and purification of a recombinant, engineered PLL, SsoPox W263F, expressed in E...
January 11, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28074221/exploring-the-thermostable-properties-of-halohydrin-dehalogenase-from-agrobacterium-radiobacter-ad1-by-a-combinatorial-directed-evolution-strategy
#16
Zhiyun Wu, Wenfeng Deng, Yapei Tong, Qian Liao, Dongmin Xin, Huashun Yu, Juan Feng, Lixia Tang
As a crucial factor for biocatalysts, protein thermostability often arises from a combination of factors that are often difficult to rationalize. In this work, the thermostable nature of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) was systematically explored using a combinatorial directed evolution approach. For this, a mutagenesis library of HheC mutants was first constructed using error-prone PCR with low mutagenesis frequency. After screening approximately 2000 colonies, six mutants with eight mutation sites were obtained...
January 10, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28073082/vibrio-parahaemolyticus-o4-k8-forms-a-potential-predominant-clone-in-southern-china-as-detected-by-whole-genome-sequence-analysis
#17
Baisheng Li, Xingfen Yang, Hailing Tan, Bixia Ke, Dongmei He, Changwen Ke, Yonghui Zhang
Vibrio parahaemolyticus has been the most common food-borne pathogen in southern China, especially the O3:K6 pandemic clone and its serovariants. Recently, the serotype O4:K8 became more and more prevalent in southern China, which was different from the O3:K6 pandemic clone. Thus, the aim of the present work was to elucidate the molecular characteristics of the O4:K8. Some O3:K6 pandemic clone and its serovariants isolated in the same period were selected for comparative analysis, which were still dominant clone locally...
January 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28071804/differential-scanning-calorimetry-analysis-of-the-effects-of-heat-and-pressure-on-protein-denaturation-in-soy-flour-mixed-with-various-types-of-plasticizers
#18
Meera Kweon, Louise Slade, Harry Levine
The effects of heat and pressure on protein denaturation in soy flour were explored by an experimental design that used pressure (atmospheric to 600 MPa), temperature (room to 90 °C), time (1 to 60 min), and type of aqueous plasticizer (NaCl, sucrose, betaine, and lactobionic acid (LBA)) as factors. When 50% (w/w) soy flour-water paste was high hydrostatic pressure (HHP)-treated for 20 min at 25 °C, the treatment at 200 MPa showed a small effect on denaturation of only the 7S soy globulin, but the treatment at 600 MPa showed a significant effect on denaturation of both the 7S and 11S soy globulins...
January 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28069243/effect-of-combined-ultrasonic-and-alkali-pretreatment-on-enzymatic-preparation-of-angiotensin-converting-enzyme-ace-inhibitory-peptides-from-native-collagenous-materials
#19
Yuhao Zhang, Liang Ma, Luyun Cai, Yi Liu, Jianrong Li
The combined effect of ultrasonic and alkali pretreatment for the hydrolysis of native collagenous materials and release of ACE inhibitory peptides was investigated. The ultrasonic and alkali pretreatment of pig skin could accelerate the release of the ACE inhibitory peptides from the triple helix of collagen in early stages of hydrolysis. Furthermore, the pretreatment could also accelerate collapse of the triple helix and release more ACE inhibitory peptides during hydrolysis than collagen samples left untreated...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28069187/characteristics-of-pectinase-treated-with-ultrasound-both-during-and-after-the-immobilization-process
#20
Xiaobin Ma, Danli Wang, Michelle Yin, Juliet Lucente, Wenjun Wang, Tian Ding, Xingqian Ye, Donghong Liu
In this study, ultrasound was applied both during and after the immobilization process and characteristics of different immobilized pectinase samples were studied. When introduced during the immobilization process, ultrasound at an intensity of 9WmL(-1) for 20min increased the immobilization yield 92.28% more than the control. When introduced to the already immobilized pectinase, ultrasound at an intensity of 4.5WmL(-1) for 10min increased the pectinase activity by 30.05%. Results of scanning electron microscope demonstrated that ultrasound increased surface area and loosened structures of immobilized enzymes...
May 2017: Ultrasonics Sonochemistry
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