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https://www.readbyqxmd.com/read/28445758/bilayer-interactions-among-unsaturated-phospholipids-sterols-and-ceramide
#1
J Peter Slotte, Tomokazu Yasuda, Oskar Engberg, Md Abdullah Al Sazzad, Victor Hautala, Thomas K M Nyholm, Michio Murata
Using differential scanning calorimetry and lifetime analysis of trans-parinaric acid fluorescence, we have examined how cholesterol and cholesteryl phosphocholine (CholPC) affect gel-phase properties of palmitoyl ceramide (PCer) in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleyol-sn-glycero-3-phosphocholine (DOPC) bilayers. By (2)H NMR, we also measured fluid-phase interactions among these lipids using deuterated analogs of POPC, PCer, and cholesterol. The PCer-rich gel phase in POPC bilayers (9:1 molar ratio of POPC to PCer) was partially and similarly dissolved (and thermostability decreased) by both cholesterol and CholPC (sterol was present equimolar to PCer, or in fourfold excess)...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28444972/production-and-application-of-a-thermostable-lipase-from-serratia-marcescens-in-detergent-formulation-and-biodiesel-production
#2
Edgar Edurman García-Silvera, Fernando Martínez-Morales, Brandt Bertrand, Daniel Morales-Guzman, Nashbly Sarela Rosas-Galván, Renato León-Rodríguez, María Del Refugio Trejo-Hernández
In this study, extracellular lipase was produced by Serratia marcescens wild type and three mutant strains. The maximum lipase activity (80 U/mL) was obtained with the SMRG4 mutant strain using soybean oil. Using a 2(2) factorial design the lipase production increased 1.55-fold (124 U/mL) with 4% and 0.05 % soybean oil and Triton X-100, respectively. The optimum conditions for maximum lipase activity were 50°C and pH 8. However, the enzyme was active in a broad range of pH (6-10) and temperatures (5-55°C)...
April 26, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28444598/synergistic-effect-of-thermostable-%C3%AE-glucosidase-tn0602-and-cellulase-on-cellulose-hydrolysis
#3
Zhaoye Zhang, Man Wang, Renjun Gao, Xiaoxiao Yu, Guang Chen
Thermophilic enzymes have many potential benefits in industrial production with increased flexibility related to process configurations. A thermostable β-glucosidase from Thermotoga naphthophila RUK-10 was found to possess catalytic activity for cellobiose hydrolysis with a high potential for application in biomass conversion. The aggregation of cellobiose often has an inhibitory effect on cellobiohydrolases and endoglucanases during cellulose hydrolysis. The presence of β-glucosidases has a significant effect on reducing inhibition from hydrolytic products by hydrolysing the intermedia cellobiose...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444450/sgnh-hydrolase-type-esterase-domain-containing-cbes-acxe2-a-novel-and-thermostable-acetyl-xylan-esterase-from-caldicellulosiruptor-bescii
#4
Surabhi Soni, Sneha S Sathe, Annamma A Odaneth, Arvind M Lali, Sanjeev K Chandrayan
Caldicellulosiruptor bescii, the most thermophilic cellulolytic bacterium, is rich in hydrolytic and accessory enzymes that can degrade untreated biomass, but the precise role of many these enzymes is unknown. One of such enzymes is a predicted GDSL lipase or esterase encoded by the locus Athe_0553. In this study, this probable esterase named as Cbes-AcXE2 was overexpressed in Escherichia coli. The Ni-NTA affinity purified enzyme exhibited an optimum pH of 7.5 at an optimum temperature of 70 °C. Cbes-AcXE2 hydrolyzed p-nitrophenyl (pNP) acetate, pNP-butyrate, and phenyl acetate with approximately equal efficiency...
April 25, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28442601/crystal-structure-of-thermostable-alkylsulfatase-sdsap-from-pseudomonas-sp-s9
#5
Lifang Sun, Pu Chen, Yintao Su, Zhixiong Cai, Lingwei Ruan, Xun Xu, Yunkun Wu
A novel alkylsulfatase from bacterium Pseudomonas sp. S9 (SdsAP) was identified as a thermostable alkylsufatases (type III) which could hydrolyze the primary alkyl sulfate such as sodium dodecyl sulfate (SDS). Thus, it has a potential application of SDS bio-degradation. The crystal structure of SdsAP has been solved to a resolution of 1.76 Å and reveals that SdsAP contains the characteristic metallo-β-lactamase like fold domain, dimerization domain and C-terminal sterol carrier protein type 2 like (SCP-2-like) fold domain...
April 25, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28439812/amylase-assisted-extraction-and-antioxidant-activity-of-polysaccharides-from-gracilaria-lemaneiformis
#6
Shengjun Wu, Mingsheng Lu, Shujun Wang
Gracilaria lemaneiformis (G. lemaneiformis) is rich in polysaccharides that have many functional activities. In this study, the thermostable α-amylase-assisted extraction and antioxidant activity of water-soluble polysaccharides from G. lemaneiformis were investigated. The extraction conditions were optimized as follows: time, 40 min; temperature, 95 °C; pH, 5 and enzyme amount, 6000 U/g, under which the yield of G. lemaneiformis polysaccharides (GPs) reached 49.15% dry base. The GPs were characterized by monosaccharide composition, FTIR spectrum, UV spectrum and antioxidant activities...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28439124/characterisation-of-a-cell-free-synthesised-g-protein-coupled-receptor
#7
Patrick J Shilling, Fabian Bumbak, Daniel J Scott, Ross A D Bathgate, Paul R Gooley
G-protein coupled receptors are the largest family of integral membrane proteins found within the human genome. They function as receptors and modulators to a wide range of ligands and responses which are crucial for human health. GPCR study, specifically the investigation of structure and interaction to cognate ligands, is of high priority. Limitations for structural study can be traced in part, to obtaining suitable quantities of recombinant protein. We sought to address the limitations of traditional recombinant technologies by utilising an Escherichia coli based cell-free protein synthesis (CFPS) approach for production of a thermostable neurotensin receptor 1 (en2NTS1)...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28438408/spray-dried-human-and-chimpanzee-adenoviral-vectored-vaccines-are-thermally-stable-and-immunogenic-in-vivo
#8
Sam Afkhami, Daniel A LeClair, Siamak Haddadi, Rocky Lai, Steven P Toniolo, Hildegund C Ertl, Emily D Cranston, Michael R Thompson, Zhou Xing
Cold chain-free vaccine technologies are needed to ensure effective vaccine delivery and coverage, particularly in resource-poor countries. However, the immunogenicity and thermostability of spray dried live viral vector-based vaccines such as recombinant adenoviral-vectored vaccines remain to be investigated. To address this issue, we have spray dried human adenoviral (AdHu5)- and chimpanzee adenoviral (AdCh68)-vectored tuberculosis vaccines in a mannitol and dextran matrix. Spray dried powders containing these two vaccines display the morphologic and chemical properties desired for long-term thermostability and vaccination...
April 21, 2017: Vaccine
https://www.readbyqxmd.com/read/28437019/extending-unique-1d-borate-chains-to-3d-framework-by-introducing-metallic-nodes
#9
Guo-Yu Yang, Qi Wei, Shi-Jia Sun, Jie Zhang
Two novel alkali/alkaline-earth borates, Ba6[B6O9 (OH)6]2(H3BO3) (1) and Li7MAlB12O24 (M = Ba, Sr, Ca) (2a-c), with 1- and 3-D structures have been hydrothermally made and characterized. 1 features a rarely 1-D anionic chain built by hexaborate clusters of B6O11(OH)6 made of six BO4/ BO2(OH)2 tetrahedra. The anionic chains are embedded in the channels of Ba6-based wheel cluster open-framework. On the basis of the structural analyses of 1, by incorporating the rational Al atoms as the linkers and tuning the reaction conditions, the anhydrous borates 2a-c have been success-fully obtained, showing novel 3-D aluminoborates (ABOs) constructed from B6O14-based cluster chains and AlO6 octa-hedra...
April 24, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28434688/stability-of-heat-stable-live-attenuated-rotavirus-vaccine-rotasiil%C3%A2
#10
Sameer P Naik, Jagdish K Zade, Rajendra N Sabale, Sambhaji S Pisal, Ravi Menon, Subhash G Bankar, Sunil Gairola, Rajeev M Dhere
Vaccines currently available across the globe are stored and transported in a continuous cold-chain at 2-8°C or below -20°C. A temperature excursion outside this range affects the potency of the vaccines. Such vaccines need to be discarded leading wastage. The Rotavirus disease burden is predominantly reported in developing and low-income countries and therefore, has entered or poised to enter their national immunization programs. These countries already have several limitations for effective storage, maintenance and distribution of vaccines in a cold-chain and this introduction is expected to further stress this fragile ecosystem...
April 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28433193/regulation-of-the-catalytic-behavior-of-pullulanases-chelated-onto-nickel-ii-modified-magnetic-nanoparticles
#11
Jianfeng Wang, Zhongmei Liu, Zhemin Zhou
Chelating of pullulanases onto nickel (II)-modified magnetic nanoparticles results in one-step purification and immobilization of pullulanase, and facilitates the commercial application of pullulanase in industrial scale. To improve the catalytic behavior, especially the operational stability, of the nanocatalyst in consecutive batch reactions, we prepared various iminodiacetic acid-modified magnetic nanoparticles differed in surface polarity and spacer length, on which the His6-tagged pullulanases were chelated via nickel ions, and then studied the correlation between the MNPs surface property and the corresponding catalyst behavior...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28433186/characterization-and-thermal-inactivation-kinetics-of-highly-thermostable-ramie-leaf-%C3%AE-amylase
#12
Liqin He, Sung-Hoon Park, Nguyen Dang Hai Dang, Hoa Xo Duong, Thi Phung Cac Duong, Phuong Lan Tran, Jong-Tae Park, Li Ni, Kwan-Hwa Park
We characterized ramie leaf β-amylase, and determined its thermostability and kinetic parameters. The enzyme was purified 53-fold using ammonium sulfate fractionation (40-60% saturation), anion exchange chromatography on DEAE-cellulose and gel permeation chromatography on Superdex-200. The purified enzyme was identified as β-amylase with molecular mass of 42kD. The enzyme displayed Km and kcat values for soluble potato starch of 1.1mg/mL and 7.8s(-1), respectively. The enzyme had a temperature optimum of 65°C, and its activity at 70°C was 92% of that at the optimal temperature after a 15-min incubation...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28431813/evaluation-of-immune-responses-of-stabilised-sat2-antigens-of-foot-and-mouth-disease-in-cattle
#13
Katherine A Scott, N M Rathogwa, A V Capozzo, Francois F Maree
Foot-and-mouth disease (FMD) vaccines with improved stability and less reliant on a cold-chain are needed to improve the longevity of immune responses elicited in animals. This is especially so for serotypes O and SAT2 which are unstable in mildly acidic pH conditions or at elevated temperatures leading to dissociation of the capsid (146S particle) and loss of immunogenicity. Previously, stabilised SAT2 viruses were generated by reverse genetic approaches and assessed in vitro and in vivo with a guinea pig trial...
April 18, 2017: Vaccine
https://www.readbyqxmd.com/read/28431258/cage-and-linear-structured-polysiloxane-epoxy-hybrids-for-coatings-surface-property-and-film-permeability
#14
Yanli Ma, Ling He, Mengjun Jia, Lingru Zhao, Yanyan Zuo, Pingan Hu
Three polysiloxane/epoxy hybrids obtained by evolving cage- or linear-structured polysiloxane into poly glycidyl methacrylate (PGMA) matrix are compared used as coatings. One is the cage-structured hybrid of P(GMA/MA-POSS) copolymer obtained by GMA and methacrylisobutyl polyhedral oligomeric silsesquioxane (MA-POSS) via free radical polymerization, the other two are PGMA/NH2-POSS and PGMA/NH2-PDMS hybrids by cage-structured aminopropyllsobutyl POSS (NH2-POSS) or linear-structured diamino terminated poly(dimethylsiloxane) (NH2-PDMS) to cure PGMA...
April 12, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28428127/potassium-and-sodium-ions-enhance-the-activity-and-thermostability-of-1-4-%C3%AE-glucan-branching-enzyme-from-geobacillus-thermoglucosidasius-in-the-presence-of-glycerol
#15
Xiaofeng Ban, Abhishek S Dhoble, Caiming Li, Yuzhu Zhang, Zhengbiao Gu, Li Cheng, Yan Hong, Zhaofeng Li
Metal ions are essential for the performance of metal-dependent proteins and are known to be important for thermophilic proteins containing a large number of charged residues. The performance of thermophilic proteins may be influenced by metal ions through electrostatic interactions between the metal ions and charged residues. In this study, we investigated the effects of metal ions and glycerol on the activity and stability of the thermophilic 1,4-α-glucan branching enzyme (abbreviated GBE; EC 2.4.1.18) from G...
April 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28427840/aav-id-a-rapid-and-robust-assay-for-batch-to-batch-consistency-evaluation-of-aav-preparations
#16
Simon Pacouret, Mohammed Bouzelha, Rajani Shelke, Eva Andres-Mateos, Ru Xiao, Anna Maurer, Mathieu Mevel, Heikki Turunen, Trisha Barungi, Magalie Penaud-Budloo, Frédéric Broucque, Véronique Blouin, Philippe Moullier, Eduard Ayuso, Luk H Vandenberghe
Adeno-associated virus (AAV) vectors are promising clinical candidates for therapeutic gene transfer, and a number of AAV-based drugs may emerge on the market over the coming years. To insure the consistency in efficacy and safety of any drug vial that reaches the patient, regulatory agencies require extensive characterization of the final product. Identity is a key characteristic of a therapeutic product, as it ensures its proper labeling and batch-to-batch consistency. Currently, there is no facile, fast, and robust characterization assay enabling to probe the identity of AAV products at the protein level...
April 17, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28426083/energetic-isomers-of-1-2-4-5-tetrazine-bis-1-2-4-triazoles-with-low-toxicity
#17
A Shlomovich, T Pechersky, A Cohen, Q L Yan, M Kosa, N Petrutik, N Tal, A Aizikovich, M Gozin
A series of nitrogen-rich "green" Energetic Materials (EMs), some with improved sensitivity, thermostability, and very low toxicity, were synthesized on the basis of 3,5-diamino-1,2,4-triazole (DAT) and 1,2,4,5-tetrazine building blocks. Since DAT contains several nucleophilic reactive sites, obtaining selective reactivity and specific isomeric products with 1,2,4,5-tetrazine precursors is a challenging task. We developed reaction conditions under which specific isomers could be prepared. On evaluating these compounds for their energetic properties, we found that N(5),N(5)'-(1,2,4,5-tetrazine-3,6-diyl)-bis(1H-1,2,4-triazole-3,5-diamine) 1 has very high thermostability (onset of decomposition temperature at 357 °C) and it is insensitive to impact (Im > 98 J), friction (>360 N) and electrostatic discharge (2512 mJ)...
April 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28423051/biochemical-and-molecular-characterization-of-the-isocitrate-dehydrogenase-with-dual-coenzyme-specificity-from-the-obligate-methylotroph-methylobacillus-flagellatus
#18
Anastasia Y Romkina, Michael Y Kiriukhin
The isocitrate dehydrogenase (MfIDH) with unique double coenzyme specificity from Methylobacillus flagellatus was purified and characterized, and its gene was cloned and overexpressed in E. coli as a fused protein. This enzyme is homodimeric,-with a subunit molecular mass of 45 kDa and a specific activity of 182 U mg -1 with NAD+ and 63 U mg -1 with NADP+. The MfIDH activity was dependent on divalent cations and Mn2+ enhanced the activity the most effectively. MfIDH exhibited a cofactor-dependent pH-activity profile...
2017: PloS One
https://www.readbyqxmd.com/read/28422151/determinants-of-thermostability-in-serine-hydroxymethyltransferase-identified-by-principal-component-analysis
#19
Fei Leng, Lu-Yun Wu, Chang Lu, Xian-Ming Pan
Protein thermostability has received growing attention in recent years. Little is known about the determinants of thermal resistance in individual protein families. However, it is known that the mechanism is family-dependent and not identical for all proteins. We present a multivariate statistical analysis to find the determinants of thermostability in one protein family, the serine hydroxymethyltransferase family. Based on principal component analysis, we identified three amino acid fragments as the potential determinants of thermostability...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28416654/reconstructed-ancestral-enzymes-suggest-long-term-cooling-of-earth-s-photic-zone-since-the-archean
#20
Amanda K Garcia, J William Schopf, Shin-Ichi Yokobori, Satoshi Akanuma, Akihiko Yamagishi
Paleotemperatures inferred from the isotopic compositions (δ(18)O and δ(30)Si) of marine cherts suggest that Earth's oceans cooled from 70 ± 15 °C in the Archean to the present ∼15 °C. This interpretation, however, has been subject to question due to uncertainties regarding oceanic isotopic compositions, diagenetic or metamorphic resetting of the isotopic record, and depositional environments. Analyses of the thermostability of reconstructed ancestral enzymes provide an independent method by which to assess the temperature history inferred from the isotopic evidence...
April 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
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