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https://www.readbyqxmd.com/read/28346347/biosynthesis-of-%C3%AE-glucosidase-inhibitors-by-a-newly-isolated-bacterium-paenibacillus-sp-tku042-and-its-effect-on-reducing-plasma-glucose-in-a-mouse-model
#1
Van Bon Nguyen, Anh Dzung Nguyen, Yao-Haur Kuo, San-Lang Wang
Paenibacillus sp. TKU042, a bacterium isolated from Taiwanese soil, produced α-glucosidase inhibitors (aGIs) in the culture supernatant when commercial nutrient broth (NB) was used as the medium for fermentation. The supernatant of fermented NB (FNB) showed stronger inhibitory activities than acarbose, a commercial anti-diabetic drug. The IC50 and maximum α-glucosidase inhibitory activities (aGIA) of FNB and acarbose against α-glucosidase were 81 μg/mL, 92% and 1395 μg/mL, 63%, respectively. FNB was found to be strongly thermostable, retaining 95% of its relative activity, even after heating at 100 °C for 30 min...
March 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28343923/first-characterization-of-an-archaeal-amino-acid-racemase-with-broad-substrate-specificity-from-the-hyperthermophile-pyrococcus-horikoshii-ot-3
#2
Ryushi Kawakami, Haruhiko Sakuraba, Taketo Ohmori, Toshihisa Ohshima
A novel amino acid racemase with broad substrate specificity (BAR) was recently isolated from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3. Characterization of this enzyme has been difficult, however, because the recombinant enzyme is produced mainly as an inclusion body in Escherichia coli. In this study, expression of the recombinant protein into the soluble fraction was markedly improved by co-expression with chaperone molecules. The purified enzyme retained its full activity after incubation at 80°C for at least 2 h in buffer (pH 7-10), making this enzyme the most thermostable amino acid racemase so far known...
March 24, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28340725/biomonitored-uhplc-esi-qtof-ms-2-and-hplc-uv-thermostability-study-of-the-aerial-parts-of-sphagneticola-trilobata-l-pruski-asteraceae
#3
Keline Lang, Jessica Corrêa, Fellippe Wolff, Gislaine Franciele da Silva, Angela Malheiros, Valdir Cechinel Filho, Ruth Meri Lucinda Silva, Nara Lins Meira Quintão, Louis P Sandjo, Tiago J Bonomini, Tania Mari Bellé Bresolin
As the temperature of extraction and processing could impact the biological effects of herbal extracts, which have wide chemical diversity, the aim of this work were to investigate the thermostability of herbal derivatives of the aerial parts of Sphagneticola trilobata, a reputed medicinal plant; to biomonitor its oral anti-hyperalgesic effect; and to elucidate the degradation pathways of the major components by UHPLC-ESI-QTOF-MS. The lipophilic markers (kaurenoic acid-KA) and hydrophilic markers [chlorogenic acids; measured as total phenolic compounds (PC), expressed in caffeic acid] were also monitored through a validated HPLC-UV methodology, suitable for quality control and stability studies...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28339473/characterization-of-lignocellulolytic-activities-from-fungi-isolated-from-the-deep-sea-sponge-stelletta-normani
#4
Ramón Alberto Batista-García, Thomas Sutton, Stephen A Jackson, Omar Eduardo Tovar-Herrera, Edgar Balcázar-López, María Del Rayo Sánchez-Carbente, Ayixon Sánchez-Reyes, Alan D W Dobson, Jorge Luis Folch-Mallol
Extreme habitats have usually been regarded as a source of microorganisms that possess robust proteins that help enable them to survive in such harsh conditions. The deep sea can be considered an extreme habitat due to low temperatures (<5°C) and high pressure, however marine sponges survive in these habitats. While bacteria derived from deep-sea marine sponges have been studied, much less information is available on fungal biodiversity associated with these sponges. Following screening of fourteen fungi isolated from the deep-sea sponge Stelletta normani sampled at a depth of 751 metres, three halotolerant strains (TS2, TS11 and TS12) were identified which displayed high CMCase and xylanase activities...
2017: PloS One
https://www.readbyqxmd.com/read/28338903/disulfide-bridges-as-essential-elements-for-the-thermostability-of-lytic-polysaccharide-monooxygenase-lpmo10c-from-streptomyces-coelicolor
#5
Magali Tanghe, Barbara Danneels, Matthias Last, Koen Beerens, Ingeborg Stals, Tom Desmet
Lytic polysaccharide monooxygenases (LPMOs) are crucial components of cellulase mixtures but their stability has not yet been studied in detail, let alone been engineered for industrial applications. In this work, we have evaluated the importance of disulfide bridges for the thermodynamic stability of Streptomyces coelicolor LPMO10C. Interestingly, this enzyme was found to retain 34% of its activity after 2-h incubation at 80°C while its apparent melting temperature (Tm) is only 51°C. When its three disulfide bridges were broken, however, irreversible unfolding occurred and no residual activity could be detected after a similar heat treatment...
March 9, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28337436/the-lid-domain-in-lipases-structural-and-functional-determinant-of-enzymatic-properties
#6
REVIEW
Faez Iqbal Khan, Dongming Lan, Rabia Durrani, Weiqian Huan, Zexin Zhao, Yonghua Wang
Lipases are important industrial enzymes. Most of the lipases operate at lipid-water interfaces enabled by a mobile lid domain located over the active site. Lid protects the active site and hence responsible for catalytic activity. In pure aqueous media, the lid is predominantly closed, whereas in the presence of a hydrophobic layer, it is partially opened. Hence, the lid controls the enzyme activity. In the present review, we have classified lipases into different groups based on the structure of lid domains...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28335316/polymer-nanocomposite-film-with-metal-rich-surface-prepared-by-in-situ-single-step-formation-of-palladium-nanoparticles-an-interesting-way-to-combine-specific-functional-properties
#7
David Thompson, David Kranbuehl, Eliane Espuche
This paper presents a continuous single-step route that permits preparation of a thermostable polymer/metal nanocomposite film and to combine different functional properties in a unique material. More precisely, palladium nanoparticles are in situ generated in a polyimide matrix thanks to a designed curing cycle which is applied to a polyamic acid/metal precursor solution cast on a glass plate. A metal-rich surface layer which is strongly bonded to the bulk film is formed in addition to homogeneously dispersed metal nanoparticles...
October 18, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335205/photosensitizer-embedded-polyacrylonitrile-nanofibers-as-antimicrobial-non-woven-textile
#8
Sarah L Stanley, Frank Scholle, Jiadeng Zhu, Yao Lu, Xiangwu Zhang, Xingci Situ, Reza A Ghiladi
Toward the objective of developing platform technologies for anti-infective materials based upon photodynamic inactivation, we employed electrospinning to prepare a non-woven textile comprised of polyacrylonitrile nanofibers embedded with a porphyrin-based cationic photosensitizer; termed PAN-Por((+)). Photosensitizer loading was determined to be 34.8 nmol/mg material; with thermostability to 300 °C. Antibacterial efficacy was evaluated against four bacteria belonging to the ESKAPE family of pathogens (Staphylococcus aureus; vancomycin-resistant Enterococcus faecium; Acinetobacter baumannii; and Klebsiella pneumonia), as well as Escherichia coli...
April 20, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335026/melting-temperature-highlights-functionally-important-rna-structure-and-sequence-elements-in-yeast-mrna-coding-regions
#9
Fei Qi, Dmitrij Frishman
Secondary structure elements in the coding regions of mRNAs play an important role in gene expression and regulation, but distinguishing functional from non-functional structures remains challenging. Here we investigate the dependence of sequence-structure relationships in the coding regions on temperature based on the recent PARTE data by Wan et al. Our main finding is that the regions with high and low thermostability (high Tm and low Tm regions) are under evolutionary pressure to preserve RNA secondary structure and primary sequence, respectively...
March 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28332816/fluorinated-and-thermo-crosslinked-polyhedral-oligomeric-silsesquioxanes-new-organic-inorganic-hybrid-materials-for-high-performance-dielectric-application
#10
Jiajia Wang, Jing Sun, Junfeng Zhou, Kaikai Jin, Qiang Fang
A fluorinated and thermo-crosslinked polyhedral oligomeric silsesquioxane (POSS) has been successfully synthesized by thermal polymerization of a fluorinated POSS monomer having an inorganic silsesquioxane core and organic side chains bearing thermo-crosslinkable trifluorovinyl ether groups. This new inorganic-organic hybrid polymer shows high thermostability with a 5 wt% loss temperature of 436 C, as well as good transparency (a sheet with an average thickness of 1.5 mm shows high transmittance of 92% varying from 400 to 1,100 nm)...
March 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28332030/molecular-cloning-expression-and-characterization-of-pectin-methylesterase-ctpme-from-clostridium-thermocellum
#11
Vikky Rajulapati, Arun Goyal
Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase (CtPME) from Clostridium thermocellum belonging to family 8 carbohydrate esterase (CE8) was cloned, expressed and purified. The amino acid sequence of CtPME exhibited similarity with pectin methylesterase from Erwinia chrysanthemi with 38% identity. The gene encoding CtPME was cloned into pET28a(+) vector and expressed using Escherichia coli BL21(DE3) cells...
March 22, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28330241/purification-characterization-gene-cloning-and-expression-of-gh-10-xylanase-penicillium-citrinum-isolate-hzn13
#12
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An extracellular thermostable xylanase (Xyl-IIb) produced by Penicillium citrinum isolate HZN13 was purified to homogeneity using DEAE-Sepharose, Sephadex G-100 and Bio-Gel P-60 chromatography with specific activity of 6272.7 U/mg and 19.6-fold purification. The purification revealed the occurrence of multiple forms of xylanases (Xyl-I, Xyl-IIa, Xyl-IIb and Xyl-III). The molecular mass of highly purified Xyl-IIb was ~31 kDa with SDS-PAGE. The enzyme was cellulase-free, thermostable (55-75 °C) and acidophilic (3...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330173/production-of-cellulase-from-aspergillus-terreus-ms105-on-crude-and-commercially-purified-substrates
#13
Muhammad Sohail, Aqeel Ahmad, Shakeel Ahmed Khan
Aspergillus terreus MS105 was originally isolated from soil and screened for cellulase production in the presence of various carbon sources including carboxymethyl cellulose (CMC), avicel, sigmacell, filter-paper and salicin. CMC induced the production of endoglucanase (EG) and filter-paperase while the levels of β-glucosidase (BGL) were increased when salicin was present in the medium. Nature of production medium influenced the duration of lag- and log-phase of the growth, rate of fungal dry-mass and enzyme production...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330171/purification-and-characterization-of-endo-%C3%AE-1-4-d-glucanase-from-trichoderma-harzianum-strain-hzn11-and-its-application-in-production-of-bioethanol-from-sweet-sorghum-bagasse
#14
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An acidophilic-solvent-thermostable endo β-1,4-D-glucanase produced from a potential Trichoderma harzianum strain HZN11 was purified to homogeneity by DEAE-Sepharose and Sephadex G-100 chromatography with 33.12 fold purification with specific activity of 66.25 U/mg and molecular mass of ~55 kDa. The optimum temperature and pH were 60 °C and 5.5 retaining 76 and 85 % of activity after 3 h, respectively. It showed stability between pH 4.5-6.0 and temperature between 50-70 °C indicating thermostability...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330157/biosynthesis-and-characterization-of-a-thermostable-alkali-tolerant-chitinase-from-bacillus-pumilus-jubch08-displaying-antagonism-against-phytopathogenic-fusarium-oxysporum
#15
Sourav Bhattacharya, Arijit Das, Saikat Samadder, Subbaramiah Sundara Rajan
The present investigation highlights the process parameters influencing the submerged fermentation of chitinase by Bacillus pumilus JUBCH08, purification and characterization of the enzyme and determination of antagonistic activity of the bacterium against Fusarium oxysporum. Medium supplemented with 0.5 % chitin and peptone, at initial pH 8.0, when incubated at 35 °C for 72 h favored highest chitinase production. The enzyme was purified 25.1-fold to homogeneity. The chitinase was found to be thermostable and alkali-tolerant with maximum activity at pH 8...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330151/insight-into-thermophiles-and-their-wide-spectrum-applications
#16
REVIEW
Ridhi Mehta, Paavan Singhal, Hardeep Singh, Dhanashree Damle, Anil K Sharma
The deconstruction of biomass is a pivotal process for the manufacture of target products using microbial cells and their enzymes. But the enzymes that possess a significant role in the breakdown of biomass remain relatively unexplored. Thermophilic microorganisms are of special interest as a source of novel thermostable enzymes. Many thermophilic microorganisms possess properties suitable for biotechnological and commercial use. There is, indeed, a considerable demand for a new generation of stable enzymes that are able to withstand severe conditions in industrial processes by replacing or supplementing traditional chemical processes...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330136/enhanced-production-of-thermostable-amidase-from-geobacillus-subterraneus-rl-2a-mtcc-11502-via-optimization-of-physicochemical-parameters-using-taguchi-doe-methodology
#17
Praveen Kumar Mehta, Shashi Kant Bhatia, Ravi Kant Bhatia, Tek Chand Bhalla
The specific effect of chemical and physical factors on amidase production from Geobacillus subterraneus RL-2a was investigated using design of experiments (DOE) methodology. The one-factor-at-a-time (OFAT) method was used to study the effects of carbon and nitrogen sources on amidase production. Subsequently, optimal levels of physical parameters and key media components, namely temperature, pH, sucrose, K2HPO4, NaCl, yeast, CaCl2·2H2O and MgSO4·7H2O, were determined using the Taguchi orthogonal array (OA) experimental design (DOE) methodology...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330134/phytases-from-enterobacter-and-serratia-species-with-desirable-characteristics-for-food-and-feed-applications
#18
Harpreet Kaur Kalsi, Rajveer Singh, Harcharan Singh Dhaliwal, Vinod Kumar
Phytases are enzymes of great industrial importance with wide range of applications in animal and human nutrition. These catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Addition of phytase to plant-based foods can improve its nutritional value and increase mineral bioavailability by decreasing nutritional effect of phytate. In the present investigation, 43 phytase positive bacteria on PSM plates were isolated from different sources and characterized for phytase activity...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28322945/bifunctional-role-of-leucine-300-of-firefly-luciferase-in-structural-rigidity
#19
Farzad Yousefi, Farangis Ataei, Mojtaba Mortazavi, Saman Hosseinkhani
Firefly luciferase is susceptible to thermal inactivation, thereby its intracellular half-life decreased. Previous reports indicated that L(300)R mutation (LRR mutant) in E(354)R/Arg(356) double mutant (ERR mutant) from Lampyris turkestanicus luciferase has increased its thermal stability and rigidity through induction of some ionic bonds with Asp 270 and 271. Disruption of the deduced ionic bonds in an ultra-rigid mutant of firefly luciferase did not reverse the flexibility of the protein. In this study, we investigated the effects of this residue to find the truth behind an extraordinary increase in thermal stability and rigidity of luciferase after replacement of leucine 300 by arginine based on previous reports...
March 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28320283/glutathione-dependent-formaldehyde-dehydrogenase-homolog-from-bacillus-subtilis-strain-r5-is-a-propanol-preferring-alcohol-dehydrogenase
#20
Raza Ashraf, Naeem Rashid, Saadia Basheer, Iram Aziz, Muhammad Akhtar
Genome search of Bacillus subtilis revealed the presence of an open reading frame annotated as glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase. The open reading frame consists of 1137 nucleotides corresponding to a polypeptide of 378 amino acids. To examine whether the encoded protein is glutathione-dependent formaldehyde dehydrogenase or alcohol dehydrogenase, we cloned and characterized the gene product. Enzyme activity assays revealed that the enzyme exhibits a metal ion-dependent alcohol dehydrogenase activity but no glutathione-dependent formaldehyde dehydrogenase or aldehyde dismutase activity...
January 2017: Biochemistry. Biokhimii︠a︡
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