Jingxu Guo, Alun R Coker, Steve P Wood, Jonathan B Cooper, Ronan M Keegan, Nasir Ahmad, Majida Atta Muhammad, Naeem Rashid, Muhummad Akhtar
Pullulan-hydrolysing enzymes, more commonly known as debranching enzymes for starch and other polysaccharides, are of great interest and have been widely used in the starch-saccharification industry. Type III pullulan hydrolase from Thermococcus kodakarensis (TK-PUL) possesses both pullulanase and α-amylase activities. Until now, only two enzymes in this class, which are capable of hydrolysing both α-1,4- and α-1,6-glycosidic bonds in pullulan to produce a mixture of maltose, panose and maltotriose, have been described...
April 1, 2018: Acta Crystallographica. Section D, Structural Biology