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https://www.readbyqxmd.com/read/28091627/development-of-a-single-step-immunoassay-microdevice-based-on-a-graphene-oxide-containing-hydrogel-possessing-fluorescence-quenching-and-size-separation-functions
#1
Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto
An immunoassay, which is an indispensable analytical method both in biological research and in medical fields was successfully integrated into a "single-step" by developing a microdevice composed of a graphene oxide (GO)-containing hydrogel and a poly (dimethylsiloxane) (PDMS) microchannel array with a polyethylene glycol (PEG) coating containing a fluorescently-labelled antibody. Here we used 2-hydroxyethylmethacrylate (HEMA) as a monomer that is easily, and homogeneously, mixed with GO to synthesize the hydrogel...
January 16, 2017: Analyst
https://www.readbyqxmd.com/read/28089273/high-performance-liquid-chromatography-analysis-of-100-nm-liposomal-nanoparticles-using-polymer-coated-silica-monolithic-columns-with-aqueous-mobile-phase
#2
Naoki Itoh, Arato Kimoto, Eiichi Yamamoto, Tatsuya Higashi, Tomofumi Santa, Takashi Funatsu, Masaru Kato
Recently, nanoparticles have garnered considerable attention, and the demand for a rapid and simple method for their analysis has increased accordingly. The bimodal pores (few μm- and few tens nm-sized pores) of monolithic columns were thought to be suitable for the separation of nanoparticles and small molecules; however, the residual silanol groups on the column surface resulted in the strong adsorption of liposomes and hindered their analysis. To overcome this problem, we modified the surface of the silica monolith via a two-step process and developed three silica monolithic columns coated with three different polymers: glycidyl methacrylate (GMA), 2-hydroxyethyl methacrylate (HEMA), and N-vinylpyrrolidone (VP)...
December 29, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28086862/management-of-dentin-hypersensitivity-by-national-dental-practice-based-research-network-practitioners-results-from-a-questionnaire-administered-prior-to-initiation-of-a-clinical-study-on-this-topic
#3
Dorota T Kopycka-Kedzierawski, Cyril Meyerowitz, Mark S Litaker, Sidney Chonowski, Marc W Heft, Valeria V Gordan, Robin L Yardic, Theresa E Madden, Stephanie C Reyes, Gregg H Gilbert
BACKGROUND: Dentin hypersensitivity (DH) is a common problem encountered in clinical practice. The purpose of this study was to identify the management approaches for DH among United States dentists. METHODS: One hundred eighty five National Dental Practice-Based Research Network clinicians completed a questionnaire regarding their preferred methods to diagnose and manage DH in the practice setting, and their beliefs about DH predisposing factors. RESULTS: Almost all dentists (99%) reported using more than one method to diagnose DH...
January 13, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28081493/optical-and-vibrational-properties-of-phosphorylcholine-based-contact-lenses-experimental-and-theoretical-investigations
#4
Katarzyna Filipecka, Rafał Miedziński, Maciej Sitarz, Jacek Filipecki, Małgorzata Makowska-Janusik
The Raman, MIR and UV-vis spectroscopy have been used to characterize Omafilcon A material constructing the one of the Proclear family contact lenses. The Omafilcon A is hydrogel material composed of 2-hydroxyethyl methacrylate (HEMA) and 2-methacryloyloxyethyl phosphorylcholine (PC) polymers crosslinked with ethyleneglycol dimethacrylate (EGDMA). Vibrational and electronic properties of the Omafilcon A material were also investigated by quantum chemical calculations. Experimentally obtained Raman, MIR and optical spectra were compared to the theoretical ones calculated applying RHF and DFT methodology...
January 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28078887/electrospun-regenerated-cellulose-nanofiber-membranes-surface-grafted-with-water-insoluble-poly-hema-or-water-soluble-poly-aas-chains-via-the-atrp-method-for-ultrafiltration-of-water
#5
Zhao Wang, Caitlin Crandall, Vicki L Prautzsch, Rajesh Sahadevan, Todd J Menkhaus, Hao Fong
Electrospun nanofiber membranes (ENMs) have demonstrated promising applications for water purification primarily due to high water flux and low degree of fouling. However, the equivalent/apparent pore sizes of as-electrospun ENMs are in microns/sub-microns; therefore, the ENMs can only be directly utilized for microfiltration applications. To make regenerated cellulose (RC) ENMs for ultrafiltration applications, atom transfer radical polymerization (ATRP) was studied to graft polymer chains onto the surface of RC nanofibers; in specific, the monomers of 2-hydroxyethyl methacrylate (HEMA) and sodium acrylate (AAS) were selected for surface-grafting water-insoluble and water-soluble polymer chains onto RC nanofibers, respectively...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28077032/iodine-responsive-poly-hema-pvp-hydrogel-for-self-regulating-burst-free-extended-release
#6
Jinku Xu, Peili Zhao, Yongchun Zhang
Swollen hydrogels with extended iodine release kinetics is highly desirable for burn and scald treatment. In this paper semi-interpenetrating poly(HEMA-PVP) hydrogels were prepared by radical polymerization followed by thermo-treatment to crosslink its PVP component. Incorporation of PVP component endows the hydrogels responsive to loaded iodine undergoing a reversible shrunken/swollen volume transition. This resulted in a self-regulating iodine release model, in which shrunken hydrogel at high iodine loading decreased drug diffusion thereby reducing burst release,, and then gradually swollen hydrogel as drug release ensures rapid release of dissociated drug from strong affinity sites on hydrogel backbone, achieving a burst-free extended release...
January 12, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28072853/terrestrial-macrofungal-diversity-from-the-tropical-dry-evergreen-biome-of-southern-india-and-its-potential-role-in-aerobiology
#7
Hema Priyamvada, M Akila, Raj Kamal Singh, R Ravikrishna, R S Verma, Ligy Philip, R R Marathe, L K Sahu, K P Sudheer, S S Gunthe
Macrofungi have long been investigated for various scientific purposes including their food and medicinal characteristics. Their role in aerobiology as a fraction of the primary biological aerosol particles (PBAPs), however, has been poorly studied. In this study, we present a source of macrofungi with two different but interdependent objectives: (i) to characterize the macrofungi from a tropical dry evergreen biome in southern India using advanced molecular techniques to enrich the database from this region, and (ii) to assess whether identified species of macrofungi are a potential source of atmospheric PBAPs...
2017: PloS One
https://www.readbyqxmd.com/read/28066611/performance-of-a-lateral-flow-immunochromatography-test-for-the-rapid-diagnosis-of-active-tuberculosis-in-a-large-multicentre-study-in-areas-with-different-clinical-settings-and-tuberculosis-exposure-levels
#8
Selene Manga, Rocio Perales, Maria Reaño, Lia D'Ambrosio, Giovanni Battista Migliori, Massimo Amicosante
BACKGROUND: Tuberculosis (TB) continues to cause an outsized burden of morbidity and mortality worldwide, still missing efficient and largely accessible diagnostic tools determining an appropriate control of the disease. Serological tests have the potentially to impact TB diagnosis, in particular in extreme clinical settings. METHODS: The diagnostic performances of the TB-XT HEMA EXPRESS (HEMA-EXPRESS) immunochromatographic rapid test for active TB diagnosis, based on use of multiple Mycobacterium tuberculosis (MTB) specific antigens, have been evaluated in a large study multicentre TB case-finding study, in populations with different exposure level to TB...
November 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28056532/analyzing-the-area-under-the-curve-of-pharyngeal-ph-probes-in-the-diagnosis-of-reflux-disease
#9
Joel W Jones, Kevin J Sykes, Anna Pavelonis, Hema Pappu, Amy M Nguyen, James D Garnett
OBJECTIVES: To analyze the area under the curve (AUC) from 24-hour pharyngeal pH probes and evaluate this parameter as a predictor of disease severity for laryngopharyngeal reflux. METHODS: Eighty patients met inclusion criteria of a completed 24-hour pharyngeal pH probe and Reflux Symptom Index (RSI) questionnaire. The AUC was calculated below a pH threshold of 5.5 using the trapezoidal rule. The RSI and RYAN scores were correlated with AUC values, and nonparametric tests were used for comparisons...
December 1, 2016: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28035120/heme-derived-from-corynebacterium-glutamicum-a-potential-iron-additive-for-swine-and-an-electron-carrier-additive-for-lactic-acid-bacterial-culture
#10
Su-In Choi, Jihoon Park, Pil Kim
To investigate the potential applications of bacterial heme, ALA synthase (HemA) was expressed in a Corynebacterium glutamicum HA strain that had been adaptively evolved against oxidative stress. The red pigment from the constructed strain was extracted and it exhibited the typical heme absorbance at 408 nm from the spectrum. To investigate the potential of this strain as an iron additive for swine, a prototype feed additive was manufactured in pilot scale by culturing the strain in a 5-ton fermenter followed by spray-drying the biomass with flour as an excipient (biomass: flour = 1:10 w/w)...
December 30, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28034362/araucaria-gum-novel-natural-polymer-for-controlled-drug-delivery-development-in-vitro-and-in-vivo-evaluation-of-araucaria-gum-based-matrix-tablets-for-controlled-release
#11
Divvela Hema Naga Durga, Venkata Ramana Murthy Kolapalli, Duppala Lohithasu
CONTEXT: Assessment of natural polymers in oral controlled drug delivery. OBJECTIVE: The objective of the present investigation was to the assessment of Araucaria gum (AHG) obtained from bark exudates of the Araucaria heterophylla (family: Araucariaceae) for the design of oral controlled release forms, in particular tablet dosage forms by using matrix systems. Diclofenac sodium was used as model drug and compared with the marketed formulation. METHODS: Matrix tablets were prepared by wet granulation technique and 5% w/v of AHG dispersion water was used as granulating agent for preparation of AHG matrix tablets...
December 30, 2016: Current Drug Delivery
https://www.readbyqxmd.com/read/28028962/assessing-the-penetrating-abilities-of-experimental-preparation-with-dental-infiltrant-features-using-optical-microscope-preliminary-study
#12
Małgorzata Skucha-Nowak, Agnieszka Machorowska-Pieniążek, Marta Tanasiewicz
BACKGROUND: The aim of the infiltration technique is to penetrate demineralized enamel with a low viscosity resin. Icon® (DMG) is the first ever and so far the only dental infiltrant. Bacteriostaticity is one of the properties that should be inherent in dental infiltrants, but Icon lacks this feature. OBJECTIVES: The aim of the preliminary study was to properly choose a dye which would allow us to assess the penetrating abilities of our own, experimental preparation with features of a dental infiltrant with bacteriostatic properties and to compare using an optical microscope the depth of infiltration of the designed experimental preparation with the infiltrant available on the market...
September 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28026935/thermoresponsive-semi-ipn-hydrogel-microfibers-from-continuous-fluidic-processing-with-high-elasticity-and-fast-actuation
#13
Yan Liu, Kaihuan Zhang, Jinghong Ma, G Julius Vancso
Hydrogels with rapid and strong response to external stimuli and possessing high elasticity and strength have been considered as platform materials for numerous applications, e.g., in biomaterials engineering. Thermoresponsive hydrogels based on semi-interpenetrating polymer networks (semi-IPN) featuring N-isopropylacrylamide with copolymers of poly(N-isopropylacrylamide-co-hydroxyethyl methacrylate) p(NIPAM-HEMA) chains are prepared and described. The copolymer was characterized by FTIR, NMR, and GPC. The semi-IPN structured hydrogel and its responsive properties were evaluated by dynamic mechanical measurements, SEM, DSC, equilibrium swelling ratio, and dynamic deswelling tests...
December 27, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28024202/microcontact-imprinted-quartz-crystal-microbalance-nanosensor-for-protein-c-recognition
#14
Monireh Bakhshpour, Erdoğan Özgür, Nilay Bereli, Adil Denizli
Detection of protein C (PC) in human serum was performed by quartz crystal microbalance (QCM) based on molecular imprinting technique (MIP). The high-resolution and mass-sensitive QCM based sensor was integrated with high sensitivity and selectivity of the MIP technique. The PC microcontact imprinted (PC-μCIP) nanofilm was prepared on the glass surface. Then, the PC-μCIP/QCM sensor was prepared with 2-hydroxyethyl methacrylate (HEMA), ethylene glycol dimethacrylate (EGDMA) and N-methacryloyl l-histidine methylester (MAH) as the functional monomer with copper(II) ions...
December 15, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28005406/a-report-on-molecular-diagnostic-testing-for-inherited-retinal-dystrophies-by-targeted-genetic-analyses
#15
Hema L Ramkumar, Harini V Gudiseva, Kameron T Kishaba, John J Suk, Rohan Verma, Keerti Tadimeti, John A Thorson, Radha Ayyagari
AIM: To test the utility of targeted sequencing as a method of clinical molecular testing in patients diagnosed with inherited retinal degeneration (IRD). METHODS: After genetic counseling, peripheral blood was drawn from 188 probands and 36 carriers of IRD. Single gene testing was performed on each patient in a Clinical Laboratory Improvement Amendment (CLIA) certified laboratory. DNA was isolated, and all exons in the gene of interest were analyzed along with 20 base pairs of flanking intronic sequence...
December 22, 2016: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28003511/tobacco-consumption-and-toxicant-exposure-of-cigarette-smokers-using-electronic-cigarettes
#16
Kim Pulvers, Ashley S Emami, Nicole L Nollen, Devan R Romero, David R Strong, Neal L Benowitz, Jasjit S Ahluwalia
BACKGROUND: There is considerable debate about the benefits and risks of electronic cigarettes (ECs). To better understand the risk-benefit ratio of ECs, more information is needed about net nicotine consumption and toxicant exposure of cigarette smokers switching to ECs. METHODS: Forty cigarette smokers (>1 year of smoking) interested in switching to ECs but not necessarily quitting smoking were enrolled in a four-week observational study and provided an e-Go C non-variable battery and refillable atomizers and choice of 8 flavors in 12 or 24 mg nicotine dosage...
December 21, 2016: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
https://www.readbyqxmd.com/read/28003196/an-expanded-transposon-mutant-library-reveals-that-vibrio-fischeri-%C3%AE-aminolevulinate-auxotrophs-can-colonize-euprymna-scolopes
#17
Noreen L Lyell, Alecia N Septer, Anne K Dunn, Drew Duckett, Julie L Stoudenmire, Eric V Stabb
: Libraries of defined mutants are valuable research tools but necessarily lack gene knockouts that are lethal under the conditions used in library construction. Here we augmented a Vibrio fischeri mutant library generated on a rich medium (LBS) by selecting transposon-insertion mutants on supplemented LBS and screening for those unable to grow on LBS. We isolated strains with insertions in alr, glr (murI), glmS, several heme-biosynthesis genes, and ftsA, as well as a mutant disrupted fourteen base pairs upstream of ftsQ Mutants with insertions in ftsA or upstream of ftsQ were recovered by addition of Mg(2+) to LBS, but their cell morphology and motility were affected...
December 21, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28000228/differential-regulation-of-ssb-genes-in-the-nitrogen-fixing-cyanobacterium-anabaena-sp-strain-pcc7120
#18
Anurag Kirti, Arvind Kumar, Hema Rajaram
Anabaena sp. PCC7120 possesses three genes coding for single-stranded DNA-binding (SSB) protein, of which ssb1 was a single gene, and ssb2 and ssb3, the first gene of their corresponding operons. Regulation of the truncated ssb genes, ssb1 (alr0088) and ssb2 (alr7559) were unaffected by N-status of growth. They were negatively regulated by the SOS-response regulatory protein LexA, as indicated by the (i) binding of Anabaena LexA to the LexA-box of regulatory regions of ssb1 and ssb2, and (ii) decreased expression of the downstream gfp reporter gene in E...
December 21, 2016: Journal of Phycology
https://www.readbyqxmd.com/read/27994525/changing-the-30-min-rule-in-canada-the-effect-of-room-temperature-on-bacterial-growth-in-red-blood-cells
#19
Sandra Ramirez-Arcos, Yuntong Kou, Éric Ducas, Louis Thibault
BACKGROUND: To maintain product quality and safety, the '30-min rule' requires the discard of red blood cells (RBCs) that are exposed to uncontrolled temperatures for more than 30 min. Recent studies suggest this rule may safely be extended to a 60-min rule. METHODS: A pool-and-split design study (N = 4) was run in parallel at Canadian Blood Services (SAGM RBCs) and Héma-Québec (AS-3 RBCs). RBCs were spiked with ∼1 colony-forming unit/ml of mesophilic and psychrophilic bacteria...
November 2016: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/27990138/purification-of-antilisterial-peptide-subtilosin-a-from-novel-bacillus-tequilensis-fr9-and-demonstrate-their-pathogen-invasion-protection-ability-using-human-carcinoma-cell-line
#20
Rizwana Parveen Rani, Marimuthu Anandharaj, Subramani Hema, Ramasamy Deepika, Abraham David Ravindran
This study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (Gallus gallus domesticus). Fifty seven colonies were isolated and three isolates (FR4, FR9, and FR12) were selected and identified as Lactobacillus gasseri FR4, Bacillus tequilensis FR9, and L. animalis FR12 by 16S rRNA sequencing. Three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. Especially, FR9 exhibited maximum inhibition against Listeria monocytogenes and none of them exhibited hemolytic activity...
2016: Frontiers in Microbiology
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