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Benzo(a)pyrene

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https://www.readbyqxmd.com/read/28629854/inhibition-of-organic-anion-transporter-oat-activity-by-cigarette-smoke-condensate
#1
Katia Sayyed, Marc Le Vee, Ziad Abdel-Razzak, Olivier Fardel
Cigarette smoke condensate (CSC) has previously been shown to impair activity and expression of hepatic drug transporters. In the present study, we provided evidence that CSC also hinders activity of organic anion transporters (OATs), notably expressed at the kidney level. CSC thus cis-inhibited OAT substrate uptake in OAT1- and OAT3-transfected HEK293 cells, in a concentration-dependent manner (IC50=72.1μg/mL for OAT1 inhibition and IC50=27.3μg/mL for OAT3 inhibition). By contrast, OAT4 as well as the renal organic cation transporter (OCT) 2 were less sensitive to the inhibitory effect of CSC (IC50=351...
June 16, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28626137/novel-palm-fatty-acid-functionalized-magnetite-nanoparticles-for-magnetic-solid-phase-extraction-of-trace-polycyclic-aromatic-hydrocarbons-from-environmental-samples
#2
Siti Khalijah Mahmad Rozi, Hamid Rashidi Nodeh, Muhammad Afzal Kamboh, Ninie Suhana Abdul Manan, Sharifah Mohamad
A novel adsorbent, palm fatty acid coated magnetic Fe3O4 nanoparticles (MNP-FA) was successfully synthesized with immobilization of the palm fatty acid onto the surface of MNPs. The successful synthesis of MNP-FA was further confirmed by X-Ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and Energy dispersive X-Ray spectroscopy (EDX) analyses and water contact angle (WCA) measurement. This newly synthesized MNP-FA was applied as magnetic solid phase extraction (MSPE) adsorbent for the enrichment of polycyclic aromatic hydrocarbons (PAHs), namely fluoranthene (FLT), pyrene (Pyr), chrysene (Cry) and benzo(a)pyrene (BaP) from environmental samples prior to High Performance Liquid Chromatography- Diode Array Detector (HPLC-DAD) analysis...
June 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28616736/geographic-variations-in-female-breast-cancer-incidence-in-relation-to-ambient-air-emissions-of-polycyclic-aromatic-hydrocarbons
#3
Courtney Large, Yudan Wei
A significant geographic variation of breast cancer incidence exists, with incidence rates being much higher in industrialized regions. The objective of the current study was to assess the role of environmental factors such as exposure to ambient air pollution, specifically carcinogenic polycyclic aromatic hydrocarbons (PAHs) that may be playing in the geographic variations in breast cancer incidence. Female breast cancer incidence and ambient air emissions of PAHs were examined in the northeastern and southeastern regions of the USA by analyzing data from the Surveillance, Epidemiology, and End Results (SEER) Program and the State Cancer Profiles of the National Cancer Institute and from the Environmental Protection Agency...
June 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28613844/assessment-of-dna-binding-and-oxidative-dna-damage-by-acrylonitrile-in-two-rat-target-tissues-of-carcinogenicity-implications-for-mechanism-of-action
#4
Gary Williams, Tetyana Kobets, Jian Dong Duan, Michael J Iatropoulos
Exposure to acrylonitrile induces formation of tumors at multiple sites in rats, with females being more sensitive. The present study assessed possible mechanisms of acrylonitrile tumorigenicity, covalent DNA binding, DNA breakage and oxidative DNA damage, in two target tissues, the brain and Zymbal's glands, of sensitive female Fischer (F344) and Sprague-Dawley (SD) rats. One group received acrylonitrile in drinking water at 100 ppm for 28 days. Two other groups were administered either acrylonitrile in drinking water at 100 ppm or drinking water alone for 27 days, followed by a single oral gavage dose of 11 mg/kg bw 14C-acrylonitrile on day 28...
June 14, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28607424/benzo-a-pyrene-triggers-desensitization-of-%C3%AE-2-adrenergic-pathway
#5
Abdullah Mayati, Normand Podechard, Manuelle Rineau, Lydie Sparfel, Dominique Lagadic-Gossmann, Olivier Fardel, Eric Le Ferrec
Exposure to environmental polycyclic aromatic hydrocarbons (PAHs), such as benzo(a)pyrene (B(a)P), has been linked to several health-threatening risks. PAHs were also shown to hinder adrenergic receptor (ADR) responses. As we previously demonstrated that B(a)P can directly interact with the β2ADR, we investigated here whether B(a)P could decrease β2ADR responsiveness by triggering receptor desensitization phenomena. We firstly showed that exposure to B(a)P reduced β2ADR-mediated epinephrine-induced induction of NR4A gene mRNAs and of intracellular cAMP...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28607059/human-genome-wide-repair-map-of-dna-damage-caused-by-the-cigarette-smoke-carcinogen-benzo-a-pyrene
#6
Wentao Li, Jinchuan Hu, Ogun Adebali, Sheera Adar, Yanyan Yang, Yi-Ying Chiou, Aziz Sancar
Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, is the major cause of lung cancer. BaP forms covalent DNA adducts after metabolic activation and induces mutations. We have developed a method for capturing oligonucleotides carrying bulky base adducts, including UV-induced cyclobutane pyrimidine dimers (CPDs) and BaP diol epoxide-deoxyguanosine (BPDE-dG), which are removed from the genome by nucleotide excision repair. The isolated oligonucleotides are ligated to adaptors, and after damage-specific immunoprecipitation, the adaptor-ligated oligonucleotides are converted to dsDNA with an appropriate translesion DNA synthesis (TLS) polymerase, followed by PCR amplification and next-generation sequencing (NGS) to generate genome-wide repair maps...
June 12, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28603847/tight-molecular-recognition-of-benzo-a-pyrene-by-a-high-affinity-antibody
#7
Andreas Eichinger, Irmgard Neumaier, Michael Pschenitza, Reinhard Nießner, Dietmar Knopp, Arne Skerra
Benzo[a]pyrene, produced during the combustion of organic material, is an abundant noxious pollutant because of its carcinogenic metabolic degradation products. The high affinity (KD ≈ 3 nM) monoclonal antibody 22F12 allows facile bioanalytical quantification of benzo[a]pyrene even in complex matrices. Here, we report its functional and X-ray crystallographic analysis in complex with 3-hydroxybenzo[a]pyrene after cloning of the V-genes and production as a recombinant Fab fragment. The polycyclic aromatic hydrocarbon is bound in a deep pocket beween the light and heavy chains surrounded mainly by aromatic and aliphatic amino acid side chains...
June 11, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28601433/characterization-of-xenobiotic-metabolizing-enzymes-of-a-reconstructed-human-epidermal-model-from-adult-hair-follicles
#8
Daniel Bacqueville, Carine Jacques, Laure Duprat, Emilien L Jamin, Beatrice Guiraud, Elisabeth Perdu, Sandrine Bessou-Touya, Daniel Zalko, Hélène Duplan
In this study, a comprehensive characterization of xenobiotic metabolizing enzymes (XMEs) based on gene expression and enzyme functionality was made in a reconstructed skin epidermal model derived from the outer root sheath (ORS) of hair follicles (ORS-RHE). The ORS-RHE model XME gene profile was consistent with native human skin. Cytochromes P450 (CYPs) consistently reported to be detected in native human skin were also present at the gene level in the ORS-RHE model. The highest Phase I XME gene expression levels were observed for alcohol/aldehyde dehydrogenases and (carboxyl) esterases...
June 7, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28599193/organic-and-inorganic-speciation-of-particulate-matter-formed-during-different-combustion-phases-in-an-improved-cookstove
#9
Anna Leavey, Sameer Patel, Raul Martinez, Dhruv Mitroo, Claire Fortenberry, Michael Walker, Brent Williams, Pratim Biswas
Residential solid fuel combustion in cookstoves has established health impacts including bladder and lung cancers, cataracts, low birth weight, and pneumonia. The chemical composition of particulate matter (PM) from 4 commonly-used solid fuels (coal, dung, ambient/dry applewood, and oakwood pellets), emitted from a gasifier cookstove, as well as propane, were examined. Temporal changes between the different cookstove burn-phases were also explored. Normalized concentrations of non-refractory PM1, total organics, chloride, ammonium, nitrate, sulfate, and 41 particle-phase polycyclic aromatic hydrocarbons (PAHs) were measured using a High-Resolution Time-of-Flight Aerosol Mass Spectrometer (HR-ToF-AMS) and a Thermal desorption Aerosol Gas chromatograph (TAG), respectively...
June 6, 2017: Environmental Research
https://www.readbyqxmd.com/read/28598261/neurotoxicity-of-alkylated-polycyclic-aromatic-compounds-in-human-neuroblastoma-cells
#10
Sailendra Nath Sarma, Jules M Blais, Hing Man Chan
Polycyclic aromatic compounds (PAC) are ubiquitous environmental pollutants originating from incomplete combustion processes. While the toxicity of parent PAC such as benzo[a]pyrene (BaP) is well characterized, effects of other alkyl-PAC dibenzothiophene (DBT) and retene (Ret) are not well established. The aim of this study was to examine the underlying relative neurotoxic mechanisms attributed to BaP (parent PAH), DBT and Ret (alkyl-PACs) using human neuroblastoma SK-N-SH cells. The lethal concentrations (LC10 and LC20) were found at approximately 10 µM and 40 µM, respectively after 24-h exposure of SK-N-SH cells...
June 9, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28594109/a-cell-free-testing-platform-to-screen-chemicals-of-potential-neurotoxic-concern-across-twenty-vertebrate-species
#11
Adeline Arini, Krittika Mittal, Peter Dornbos, Jessica Head, Jennifer Rutkiewicz, Niladri Basu
There is global demand for new in vitro testing tools for ecological risk assessment. The objective of this study was to apply a set of cell-free neurochemical assays to screen many chemicals across many species in a relatively high-throughput manner. The platform assessed seven receptors and enzymes that mediate neurotransmission of GABA, dopamine, glutamate, and acetylcholine. Each assay was optimized to work across 20 vertebrate species (5 fish, 5 birds, 7 mammalian wildlife, 3 biomedical species including humans)...
June 8, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28593498/bpde-induced-genotoxicity-relationship-between-dna-adducts-mutagenicity-in-the-in-vitro-pig-a-assay-and-the-transcriptional-response-to-dna-damage-in-tk6-cells
#12
Ann Liza Piberger, Christopher T Krüger, Bettina M Strauch, Beatrice Schneider, Andrea Hartwig
Benzo[a]pyrene is a known human carcinogen. As underlying mechanism, the induction of stable DNA adducts and mutations have been repeatedly demonstrated. Also, the activation of cellular stress response on the transcriptional level has been described. Nevertheless, the interrelationship between these different events is less well understood, especially at low, for human exposure relevant concentrations. Within the present study, we applied the reactive metabolite benzo[a]pyrene diolepoxide (BPDE) in the nanomolar, non-cytotoxic concentration range in human TK6 cells and quantified the induction and repair of stable DNA adducts at the N (2)-position of guanine by HPLC with fluorescence detection...
June 7, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28590158/relationship-between-total-polar-components-and-polycyclic-aromatic-hydrocarbons-in-fried-edible-oil
#13
Ke-Jing An, Yu-Lan Liu, Hai-Lan Liu
Deep-fried dough sticks (a Chinese traditional breakfast) were fried individually in peanut, sunflower, rapeseed, rice bran, soybean and palm oil without any time lag for 32 h (64 batches fried, each for 30 min) and fried oil samples were obtained every 2 h. The frying-induced changes in the levels of total polar compounds (TPC) and polycyclic aromatic hydrocarbons (PAHs) were investigated by edible oil polar compounds (EOPC) fast separation chromatographic system and gas chromatography-mass spectrometry (GC-MS), respectively...
June 20, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28589335/a-non-destructive-bfcod-assay-for-in-vivo-measurement-of-cytochrome-p450-3a-cyp3a-enzyme-activity-in-fish-embryos-and-larvae
#14
Elias M Oziolor, Alexis N Carey, Cole W Matson
There is increasing interest in quantifying the exposure and effects of anthropogenic contaminants in fish. Determination of exposures in wild fish is routinely performed, but methods to investigate potential effects are less established. One of the most relevant approaches would be the use of in vivo assays, but existing assays are often limited to in vitro determination of enzyme activity. Many pharmaceuticals and some persistent pollutants activate, and are metabolized by cytochrome P4503A (CYP3A), which make it a relevant and desirable target for biomarker research...
June 6, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28587153/nanoporous-gold-films-prepared-by-a-combination-of-sputtering-and-dealloying-for-trace-detection-of-benzo-a-pyrene-based-on-surface-plasmon-resonance-spectroscopy
#15
Li Wang, Xiu-Mei Wan, Ran Gao, Dan-Feng Lu, Zhi-Mei Qi
A wavelength-interrogated surface plasmon resonance (SPR) sensor based on a nanoporous gold (NPG) film has been fabricated for the sensitive detection of trace quantities of benzo[a]pyrene (BaP) in water. The large-area uniform NPG film was prepared by a two-step process that includes sputtering deposition of a 60-nm-thick AuAg alloy film on a glass substrate and chemical dealloying of the alloy film in nitric acid. For SPR sensor applications, the NPG film plays the dual roles of analyte enrichment and supporting surface plasmon waves, which leads to sensitivity enhancement...
June 1, 2017: Sensors
https://www.readbyqxmd.com/read/28585014/distribution-sources-and-ecological-risk-assessment-of-polycyclic-aromatic-hydrocarbons-in-surface-sediments-from-the-haihe-river-a-typical-polluted-urban-river-in-northern-china
#16
Xiao Qian, Baocui Liang, Xuan Liu, Xinhui Liu, Juan Wang, Fei Liu, Baoshan Cui
The distribution, sources, and ecological risk of polycyclic aromatic hydrocarbons (PAHs) were investigated in surface sediments from the Haihe River. Total PAH concentrations varied from 171.4 to 9511.2 ng g(-1) with an average of 2125.4 ng g(-1), suggesting serious pollution of the Haihe River in comparison with other reported rivers worldwide. PAH contaminants differed significantly among 17 sampling locations with high values occurring in industrial areas and densely populated areas. The composition of PAHs was characterized by high abundance of 4-ring and 5-ring PAHs, and benzo[a]anthracene, chrysene, and benzo[a]pyrene were the predominant components...
June 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28583304/transgenerational-inheritance-of-neurobehavioral-and-physiological-deficits-from-developmental-exposure-to-benzo-a-pyrene-in-zebrafish
#17
Andrea L Knecht, Lisa Truong, Skylar W Marvel, David M Reif, Abraham Garcia, Catherine Lu, Michael T Simonich, Justin G Teeguarden, Robert L Tanguay
Benzo[a]pyrene (B[a]P) is a well-known genotoxic polycylic aromatic compound whose toxicity is dependent on signaling via the aryl hydrocarbon receptor (AHR). It is unclear to what extent detrimental effects of B[a]P exposures might impact future generations and whether transgenerational effects might be AHR-dependent. This study examined the effects of developmental B[a]P exposure on 3 generations of zebrafish. Zebrafish embryos were exposed from 6 to 120h post fertilization (hpf) to 5 and 10μM B[a]P and raised in chemical-free water until adulthood (F0)...
June 3, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28583029/p-gp-expression-levels-in-the-erythrocytes-of-brown-trout-a-new-tool-for-aquatic-sentinel-biomarker-development
#18
Emeline Valton, Ivan Wawrzyniak, Christian Amblard, Bruno Combourieu, Marie-Laure Bayle, François Desmolles, Fabrice Kwiatkowski, Frédérique Penault-Llorca, Mahchid Bamdad
CONTEXT: P-glycoprotein (P-gp) is a ubiquitous membrane detoxification pump involved in cellular defence against xenobiotics. Blood is a hub for the trade and transport of physiological molecules and xenobiotics. Our recent studies have highlighted the expression of a 140-kDa P-gp in brown trout erythrocytes in primary cell culture and its dose-dependent response to Benzo[a]pyrene pollutant. OBJECTIVE: The purpose of this study was focused on using P-gp expression in brown trout erythrocytes as a biomarker for detecting the degree of river pollution...
June 19, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/28582767/effects-of-soil-water-saturation-on-sampling-equilibrium-and-kinetics-of-selected-polycyclic-aromatic-hydrocarbons
#19
Pil-Gon Kim, Ji-Yeon Roh, Yongseok Hong, Jung-Hwan Kwon
Passive sampling can be applied for measuring the freely dissolved concentration of hydrophobic organic chemicals (HOCs) in soil pore water. When using passive samplers under field conditions, however, there are factors that might affect passive sampling equilibrium and kinetics, such as soil water saturation. To determine the effects of soil water saturation on passive sampling, the equilibrium and kinetics of passive sampling were evaluated by observing changes in the distribution coefficient between sampler and soil (Ksampler/soil) and the uptake rate constant (ku) at various soil water saturations...
May 31, 2017: Chemosphere
https://www.readbyqxmd.com/read/28581717/determination-of-pahs-in-solution-with-a-single-reference-standard-by-a-combination-of-1-h-quantitative-nmr-spectroscopy-and-chromatography
#20
Yuko Kitamaki, Naoki Saito, Taichi Yamazaki, Satoko Otsuka, Satoe Nakamura, Yuzo Nishizaki, Naoki Sugimoto, Masahiko Numata, Toshihide Ihara
We applied a combination of (1)H quantitative NMR spectroscopy ((1)H-qNMR) and chromatography (GC or LC) to establish reliable analytical methods (qNMR/GC and qNMR/LC) for organic compounds. In this method, a reference standard is used as an internal standard for both (1)H-qNMR and chromatography to estimate relative molar sensitivity (RMS) for analytes. The RMSs are calculated from the molar ratios between analytes and the reference standard obtained by (1)H-qNMR; and the response ratio between them obtained by chromatography...
June 5, 2017: Analytical Chemistry
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