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https://www.readbyqxmd.com/read/28935979/intergrative-metabolomic-and-transcriptomic-analyses-unveil-nutrient-remobilization-events-in-leaf-senescence-of-tobacco
#1
Wei Li, Hailiang Zhang, Xiaoxu Li, Fengxia Zhang, Cheng Liu, Yongmei Du, Xiaoming Gao, Zenglin Zhang, Xiaobing Zhang, Zhihui Hou, Hui Zhou, Xiaofei Sheng, Guodong Wang, Yongfeng Guo
Leaf senescence in plants is a coordinated process that involves remobilization of nutrients from senescing leaves to sink tissues. The molecular events associated with nutrient remobilization are however not well understood. In this study the tobacco system with a source-sink relationship between different leaf positions was used in analyzing the spatiotemporal changes of 76 metabolites from leaves at 3 different stalk positions and 8 developmental stages. The metabolomic data was then compared with RNA-seq data from the same samples to analyze the activities of the metabolic pathways that are important for nutrient remobilization...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935625/topographic-diagnosis-of-craniopharyngiomas-the-accuracy-of-mri-findings-observed-on-conventional-t1-and-t2-images
#2
R Prieto, J M Pascual, L Barrios
BACKGROUND AND PURPOSE: The topography of craniopharyngiomas has proved fundamental in predicting the involvement of vital brain structures and the possibility of achieving a safe radical resection. Beyond the imprecise term "suprasellar," indiscriminately used for craniopharyngiomas, an accurate definition of craniopharyngioma topography should be assessed by preoperative MR imaging. The objective of this study was to investigate the MRI findings that help define craniopharyngioma topography...
September 21, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/28935481/stalking-a-lethal-superbug-by-whole-genome-sequencing-and-phylogenetics-influence-on-unraveling-a-major-hospital-outbreak-of-carbapenem-resistant-klebsiella-pneumoniae
#3
Thorsten Kaiser, Knut Finstermeier, Madlen Häntzsch, Sarah Faucheux, Martin Kaase, Tim Eckmanns, Sven Bercker, Udo X Kaisers, Norman Lippmann, Arne C Rodloff, Joachim Thiery, Christoph Lübbert
BACKGROUND: From July 2010-April 2013, Leipzig University Hospital experienced the largest outbreak of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae (KPC-2-Kp) strain observed in Germany to date. After termination of the outbreak, we aimed to reconstruct transmission pathways by phylogenetics based on whole-genome sequencing (WGS). METHODS: One hundred seventeen KPC-2-Kp isolates from 89 outbreak patients, 5 environmental KPC-2-Kp isolates, and 24 K pneumoniae strains not linked to the outbreak underwent WGS...
September 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28935332/surveillance-is-important-after-surgical-excision-of-giant-fibrovascular-polyps-of-the-esophagus
#4
Andrew J Cockbain, Ruth England, Simon P L Dexter, Abeezar I Sarela
Giant fibrovascular polyps of the esophagus are rare benign tumors arising from the cervical esophagus or hypopharynx. Radiologic and endoscopic investigation may struggle to identify a stalk or to delineate the tumor as luminal or submucosal in origin. Excision is advised, although the diagnosis, and therefore the optimal operative approach, may not be apparent until the time of operation. Individual case reports describe the technical success of surgical excision but rarely include follow-up. Our case series highlights the range of approaches for surgical excision and also the importance of long-term follow-up because of the risk of recurrence and potential for airway obstruction...
October 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28934004/development-of-functional-composts-using-spent-coffee-grounds-poultry-manure-and-biochar-through-microbial-bioaugmentation
#5
S Aalfin Emmanuel, Jangyeon Yoo, Eok-Jo Kim, Jae-Soo Chang, Young-In Park, Sung-Cheol Koh
Spent coffee grounds (SCG), poultry manure, and agricultural waste-derived biochar were used to manufacture functional composts through microbial bioaugmentation. The highest yield of tomato stalk-based biochar (40.7%) was obtained at 450°C with a surface area of 2.35 m(2) g(-1). Four pilot-scale composting reactors were established to perform composting for 45 days. The ratios of NH4(+)-N/NO3(-)-N, which served as an indicator of compost maturity, indicate rapid, and successful composting via microbial bioaugmentation and biochar amendment...
September 21, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28933100/-decipherment-of-ancient-literature-about-danshen
#6
Ai-Ping Deng, Lan-Ping Guo, Zhi-Lai Zhan, Lu-Qi Huang
Danshen has a long history in China as a commen traditional Chinese medicine, but many other members of the Salvia family is also used as Danshen in particular regions. The name of Danshen were analyzed according to the color, efficacity and etc. To investigate the real origin of Danshen, the stalk, leaf, flower and radix in chronicle and pictures of ancient literature were deciphered. It showed that in the past dynasties, Salvia miltiorrhiza was the most likely plant used as Danshen. The rigin and quality evaluation of Danshen were also summerized, provided new insight and proof for the development and utilization of Danshen...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28931087/microbial-analysis-of-zetaproteobacteria-and-co-colonizers-of-iron-mats-in-the-troll-wall-vent-field-arctic-mid-ocean-ridge
#7
Jan Vander Roost, Ingunn Hindenes Thorseth, Håkon Dahle
Over the last decade it has become increasingly clear that Zetaproteobacteria are widespread in hydrothermal systems and that they contribute to the biogeochemical cycling of iron in these environments. However, how chemical factors control the distribution of Zetaproteobacteria and their co-occurring taxa remains elusive. Here we analysed iron mats from the Troll Wall Vent Field (TWVF) located at the Arctic Mid-Ocean Ridge (AMOR) in the Norwegian-Greenland Sea. The samples were taken at increasing distances from high-temperature venting chimneys towards areas with ultraslow low-temperature venting, encompassing a large variety in geochemical settings...
2017: PloS One
https://www.readbyqxmd.com/read/28928215/influenza-virus-hemagglutinin-stalk-specific-antibodies-in-human-serum-are-a-surrogate-marker-for-in-vivo-protection-in-a-serum-transfer-mouse-challenge-model
#8
Henning Jacobsen, Madhusudan Rajendran, Angela Choi, Haakon Sjursen, Karl A Brokstad, Rebecca J Cox, Peter Palese, Florian Krammer, Raffael Nachbagauer
The immunogenicity of current influenza virus vaccines is assessed by measuring an increase of influenza virus-specific antibodies in a hemagglutination inhibition assay. This method exclusively measures antibodies against the hemagglutinin head domain. While this domain is immunodominant, it has been shown that hemagglutination inhibition titers do not always accurately predict protection from disease. In addition, several novel influenza virus vaccines that are currently under development do not target the hemagglutinin head domain, but rather more conserved sites, including the hemagglutinin stalk...
September 19, 2017: MBio
https://www.readbyqxmd.com/read/28927639/comparison-of-bio-hydrogen-production-yield-capacity-between-asynchronous-and-simultaneous-saccharification-and-fermentation-processes-from-agricultural-residue-by-mixed-anaerobic-cultures
#9
Yameng Li, Zhiping Zhang, Shengnan Zhu, Huan Zhang, Yang Zhang, Tian Zhang, Quanguo Zhang
Taken common agricultural residues as substrate, dark fermentation bio-hydrogen yield capacity from asynchronous saccharification and fermentation (ASF) and simultaneous saccharification and fermentation (SSF) was investigated. The highest hydrogen yield of 472.75mL was achieved with corncob using ASF. Hydrogen yield from corn straw, rice straw, corncob and sorghum stalk by SSF were 20.54%,10.31%,13.99% and 5.92% higher than ASF, respectively. The experimental data fitted well to the modified Gompertz model...
September 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28926818/effects-of-genetically-modified-cotton-stalks-on-antibiotic-resistance-genes-inti1-and-inti2-during-pig-manure-composting
#10
Manli Duan, Jie Gu, Xiaojuan Wang, Yang Li, Sheqi Zhang, Yanan Yin, Ranran Zhang
Genetically modified (GM) cotton production generates a large yield of stalks and their disposal is difficult. In order to study the feasibility of using GM cotton stalks for composting and the changes that occur in antibiotic resistance genes (ARGs) during composting, we supplemented pig manure with GM or non-GM cotton stalks during composting and we compared their effects on the absolute abundances (AA) of intI1, intI2, and ARGs under the two treatments. The compost was mature after processing based on the germination index and C/N ratio...
September 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28924128/igg4-related-hypophysitis-with-subtle-hypopituitarism-in-an-elderly-diabetic-patient-is-treatment-or-observation-preferable
#11
Motoki Kawasaki, Motoyoshi Tsujino, Fuminori Sato, Maya Sakurada, Kenji Nishida, Takayasu Kise, Yuko Hijioka, Mitsugu Ishizawa, Kazuaki Enatsu, Yoshihiro Ogawa
A 70-year-old man with diabetes mellitus presented with an enlarged pituitary stalk in 2014. IgG4-related parotitis and submandibular sialoadenitis were diagnosed in 2012. He denied any symptoms related to a pituitary mass. His visual field was intact, and his hypopituitarism was subtle. The serum IgG4 level was elevated. A lip biopsy revealed strong fibrosis and hyper-infiltration of IgG4-positive plasma cells. Based on these findings, IgG4-related hypophysitis was diagnosed. The patient was carefully followed without specific intervention...
September 15, 2017: Internal Medicine
https://www.readbyqxmd.com/read/28922549/asymmetric-iron-catalyzed-c-h-alkylation-enabled-by-remote-ligand-meta-substitution
#12
Joachim Loup, Daniel Zell, Joao Carlos Agostinho de Oliveira, Helena Keil, Dietmar Stalke, Lutz Ackermann
Highly enantioselective iron-catalyzed C-H alkylations by inner-sphere C-H activation were accomplished with ample scope. High levels of enantiocontrol proved viable through a novel ligand design that exploits a remote meta-substitution on N-heterocyclic carbenes within a facile LLHT C-H cleavage.
September 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28919900/preliminary-studies-to-characterize-the-temporal-variation-of-micronutrient-composition-of-the-above-ground-organs-of-maize-and-correlated-uptake-rates
#13
Karla Vilaça Martins, Durval Dourado-Neto, Klaus Reichardt, Quirijn de Jong van Lier, José Laércio Favarin, Felipe Fadel Sartori, Guilherme Felisberto, Simone da Costa Mello
The improvement of agronomic practices and the use of high technology in field crops contributes for significant increases in maize productivity, and may have altered the dynamics of nutrient uptake and partition by the plant. Official recommendations for fertilizer applications to the maize crop in Brazil and in many countries are based on critical soil nutrient contents and are relatively outdated. Since the factors that interact in an agricultural production system are dynamic, mathematical modeling of the growth process turns out to be an appropriate tool for these studies...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28917091/recovery-from-diabetes-insipidus-and-preservation-of-thyroid-function-after-craniopharyngioma-removal-and-pituitary-stalk-sectioning
#14
Yoshikazu Ogawa, Kuniyasu Niizuma, Teiji Tominaga
OBJECTIVES: Craniopharyngioma is a slow-growing tumor, but long-term tumor control with maintenance of quality of life is sometimes very difficult to achieve, and hypothalamic disturbance should be strictly avoided in the treatment. However, management of the pituitary gland and/or pituitary stalk varies among surgeons and institutions. PATIENTS AND METHODS: This retrospective review identified 44 patients, 24 males and 20 females with craniopharyngiomas who were initially treated by surgery through the extended transsphenoidal approach with pituitary stalk sectioning at a single institute...
September 9, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28916922/evaluation-of-zmcct-haplotypes-for-genetic-improvement-of-maize-hybrids
#15
Yipu Li, Lixiu Tong, Lele Deng, Qiyu Liu, Yuexian Xing, Chao Wang, Baoshen Liu, Xiaohong Yang, Mingliang Xu
The elite ZmCCT haplotypes which have no transposable element in the promoter could enhance maize resistance to Gibberella stalk rot and improve yield-related traits, while having no or mild impact on flowering time. Therefore, they are expected to have great value in future maize breeding programs. A CCT domain-containing gene, ZmCCT, is involved in both photoperiod response and stalk rot resistance in maize. At least 15 haplotypes are present at the ZmCCT locus in maize germplasm, whereas only three of them are found in Chinese commercial maize hybrids...
September 15, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28910326/sex-differences-in-the-herding-styles-of-working-sheepdogs-and-their-handlers
#16
Erin Kydd, Paul McGreevy
Working sheepdog trials test the attributes of dogs as well as the dogmanship and stockmanship skills of handlers. They generally include standard elements such as outrun, lift, fetch, drive, shed, pen and single to test all facets of the work that dogs perform on a farm. While both male and female handlers participate, these trials are traditionally dominated by male handlers. Both male and female dogs compete on equal terms within the same events. Drawing data from files (n = 60) downloaded from YouTube, the current study explores whether behaviours of dogs and their handlers during sheepdog trials differ between handler gender and dog sex at different levels of competition...
2017: PloS One
https://www.readbyqxmd.com/read/28910101/anisotropic-fluctuations-in-the-ribosome-determine-trna-kinetics
#17
Huan Yang, Jeffrey K Noel, Paul Charles Whitford
The ribosome is a large ribonucleoprotein complex that is responsible for the production of proteins in all organisms. Accommodation is the process by which an incoming aminoacyl-transfer RNA (aa-tRNA) molecule binds the ribosomal A site, and its kinetics has been implicated in the accuracy of tRNA selection. In addition to rearrangements in the aa-tRNA molecule, the L11 stalk can undergo large-scale anisotropic motions during translation. To explore the potential impact of this protruding region on the rate of aa-tRNA accommodation, we used molecular dynamics simulations with a simplified model to evaluate the free energy as a function of aa-tRNA position...
September 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28904748/the-complexity-of-mating-decisions-in-stalk-eyed-flies
#18
Nadine C Chapman, Penthai Siriwat, James Howie, Aaron Towlson, Lawrence Bellamy, Kevin Fowler, Andrew Pomiankowski
All too often, studies of sexual selection focus exclusively on the responses in one sex, on single traits, typically those that are exaggerated and strongly sexually dimorphic. They ignore a range of less obvious traits and behavior, in both sexes, involved in the interactions leading to mate choice. To remedy this imbalance, we analyze a textbook example of sexual selection in the stalk-eyed fly (Diasemopsis meigenii). We studied several traits in a novel, insightful, and efficient experimental design, examining 2,400 male-female pairs in a "round-robin" array, where each female was tested against multiple males and vice versa...
September 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28904193/mutations-in-the-fusion-protein-of-measles-virus-that-confer-resistance-to-the-membrane-fusion-inhibitors-carbobenzoxy-d-phe-l-phe-gly-and-as-48
#19
Michael N Ha, Sébastien Delpeut, Ryan S Noyce, Gary Sisson, Karen M Black, Liang-Tzung Lin, Darius Bilimoria, Richard K Plemper, Gilbert G Privé, Christopher D Richardson
The inhibitors Z-d-Phe-l-Phe Gly (fusion inhibitor peptide, FIP) and AS-48 have similar efficacy in blocking membrane fusion and syncytia formation mediated by measles virus. Other homologues such as Z-d-Phe are less effective, but may act through the same mechanism. In an attempt to map the site of action of these inhibitors, we generated mutant viruses which were resistant to the inhibitory effects of Z-d-Phe-l-Phe Gly. These 10 mutations were localized to the heptad repeat region (HRB) of the fusion protein and no changes were observed in the viral hemagglutinin, which is the receptor attachment protein...
September 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28904019/cathepsin-b-mediated-cd18-shedding-regulates-leukocyte-recruitment-from-angiogenic-vessels
#20
Shintaro Nakao, Souska Zandi, Dawei Sun, Ali Hafezi-Moghadam
Cathepsin B (CtsB) contributes to atherosclerosis and cancer progression by processing the extracellular matrix and promoting angiogenesis. Although CtsB was reported to promote and reduce angiogenesis, there is no mechanistic explanation that reconciles this apparent discrepancy. CtsB cleaves CD18 from the surface of immune cells, but its contribution to angiogenesis has not been studied. We developed an in vivo technique for visualization of immune cell transmigration from corneal vessels toward implanted cytokines...
September 13, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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