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https://www.readbyqxmd.com/read/28231124/food-safety-impacts-from-post-harvest-processing-procedures-of-molluscan-shellfish
#1
REVIEW
George L Baker
Post-harvest Processing (PHP) methods are viable food processing methods employed to reduce human pathogens in molluscan shellfish that would normally be consumed raw, such as raw oysters on the half-shell. Efficacy of human pathogen reduction associated with PHP varies with respect to time, temperature, salinity, pressure, and process exposure. Regulatory requirements and PHP molluscan shellfish quality implications are major considerations for PHP usage. Food safety impacts associated with PHP of molluscan shellfish vary in their efficacy and may have synergistic outcomes when combined...
April 18, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28230734/deep%C3%A2-transcriptome%C3%A2-sequencing%C3%A2-of%C3%A2-two%C3%A2-green%C3%A2-algae-chara%C3%A2-vulgaris%C3%A2-and%C3%A2-chlamydomonas%C3%A2-reinhardtii-%C3%A2-provides%C3%A2-no%C3%A2-evidence%C3%A2-of%C3%A2-organellar%C3%A2-rna%C3%A2-editing
#2
A Bruce Cahoon, John A Nauss, Conner D Stanley, Ali Qureshi
Nearly all land plants post-transcriptionally modify specific nucleotides within RNAs, a process known as RNA editing. This adaptation allows the correction of deleterious mutations within the asexually reproducing and presumably non-recombinant chloroplast and mitochondrial genomes. There are no reports of RNA editing in any of the green algae so this phenomenon is presumed to have originated in embryophytes either after the invasion of land or in the now extinct algal ancestor of all land plants. This was challenged when a recent in silico screen for RNA edit sites based on genomic sequence homology predicted edit sites in the green alga Chara vulgaris, a multicellular alga found within the Streptophyta clade and one of the closest extant algal relatives of land plants...
February 20, 2017: Genes
https://www.readbyqxmd.com/read/28230724/alternative-splicing-in-plant-genes-a-means-of-regulating-the-environmental-fitness-of-plants
#3
REVIEW
Xudong Shang, Ying Cao, Ligeng Ma
Gene expression can be regulated through transcriptional and post-transcriptional mechanisms. Transcription in eukaryotes produces pre-mRNA molecules, which are processed and spliced post-transcriptionally to create translatable mRNAs. More than one mRNA may be produced from a single pre-mRNA by alternative splicing (AS); thus, AS serves to diversify an organism's transcriptome and proteome. Previous studies of gene expression in plants have focused on the role of transcriptional regulation in response to environmental changes...
February 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28230438/the-influence-of-placements-on-adult-nursing-graduates-choice-of-first-post
#4
Mark Wareing, Renate Taylor, Aileen Wilson, Adrienne Sharples
BACKGROUND: This article presents findings from a study that sought to explore the extent to which clinical placements have an impact on nursing students' decisions regarding their first staff nurse post. Within the UK, nursing is facing a recruitment crisis with particular difficulty recruiting to areas such as primary care and care of older people. Transitioning into a new role is challenging in any occupation, but it is a particular problem in nursing where the realities of professional practice often differ from students' perception of the staff nurse role as shaped by their clinical placements...
February 23, 2017: British Journal of Nursing: BJN
https://www.readbyqxmd.com/read/28230180/assessment-of-chicken-carcass-microbiome-responses-during-processing-in-the-presence-of-commercial-antimicrobials-using-a-next-generation-sequencing-approach
#5
Sun Ae Kim, Si Hong Park, Sang In Lee, Casey M Owens, Steven C Ricke
The purpose of this study was to 1) identify microbial compositional changes on chicken carcasses during processing, 2) determine the antimicrobial efficacy of peracetic acid (PAA) and Amplon (blend of sulfuric acid and sodium sulfate) at a poultry processing pilot plant scale, and 3) compare microbial communities between chicken carcass rinsates and recovered bacteria from media. Birds were collected from each processing step and rinsates were applied to estimate aerobic plate count (APC) and Campylobacter as well as Salmonella prevalence...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229953/realizing-field-dependent-conduction-in-zno-nanowires-without-annealing
#6
C P Burke-Govey, U Castanet, H Warring, A Nau, B J Ruck, J Majimel, N O V Plank
We report on the low-temperature fabrication of field-effect transistors by bridging pre-patterned electrodes using ZnO nanowires grown in situ, which operate without requiring post-growth processing or annealing. The devices show good performance using as-grown nanowires, with on-off ratios of 10(5) and threshold voltages of 2 V. Electron microscopy shows the field-dependent nanowires hierarchically nucleate from larger ZnO nanorods, and both are oriented along a common c-axis. A high nanowire surface-to-volume ratio allows depleting electron traps on the nanowire surface to compensate intrinsic electron donors present throughout the nanowire bulk...
February 23, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28229923/epigenetic-regulation-of-reln-and-gad1-in-the-frontal-cortex-fc-of-autism-spectrum-disorder-asd-subjects
#7
Adrian Zhubi, Ying Chen, Alessandro Guidotti, Dennis R Grayson
Both Reelin (RELN) and glutamate decarboxylase 67 (GAD1) have been implicated in the pathophysiology of Autism Spectrum Disorders (ASD). We have previously shown that both mRNAs are reduced in the cerebella (CB) of ASD subjects through a mechanism that involves increases in the amounts of MECP2 binding to the corresponding promoters. In the current study, we examined the expression of RELN, GAD1, GAD2, and several other mRNAs implicated in this disorder in the frontal cortices (FC) of ASD and CON subjects. We also focused on the role that epigenetic processes play in the regulation of these genes in ASD brain...
February 13, 2017: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/28229915/regulated-methionine-oxidation-by-monooxygenases
#8
REVIEW
Bruno Manta, Vadim N Gladyshev
Protein function can be regulated via post-translational modifications by numerous enzymatic and non-enzymatic mechanisms, including oxidation of cysteine and methionine residues. Redox-dependent regulatory mechanisms have been identified for nearly every cellular process, but the major paradigm has been that cellular components are oxidized (damaged) by reactive oxygen species (ROS) in a relatively unspecific way, and then reduced (repaired) by designated reductases. While this scheme may work with cysteine, it cannot be ascribed to other residues, such as methionine, whose reaction with ROS is too slow to be biologically relevant...
February 13, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28229910/optimized-energy-of-spectral-ct-for-infarct-imaging-experimental-validation-with-human-validation
#9
Veit Sandfort, Srikanth Palanisamy, Rolf Symons, Amir Pourmorteza, Mark A Ahlman, Kelly Rice, Tom Thomas, Cynthia Davies-Venn, Bernhard Krauss, Alan Kwan, Ankur Pandey, Stefan L Zimmerman, David A Bluemke
OBJECTIVES: Late contrast enhancement visualizes myocardial infarction, but the contrast to noise ratio (CNR) is low using conventional CT. The aim of this study was to determine if spectral CT can improve imaging of myocardial infarction. MATERIALS AND METHODS: A canine model of myocardial infarction was produced in 8 animals (90-min occlusion, reperfusion). Later, imaging was performed after contrast injection using CT at 90 kVp/150 kVpSn. The following reconstructions were evaluated: Single energy 90 kVp, mixed, iodine map, multiple monoenergetic conventional and monoenergetic noise optimized reconstructions...
February 11, 2017: Journal of Cardiovascular Computed Tomography
https://www.readbyqxmd.com/read/28229907/expression-and-functional-evaluation-of-biopharmaceuticals-made-in-plant-chloroplasts
#10
REVIEW
Bei Zhang, Balamurugan Shanmugaraj, Henry Daniell
After approval of the first plant-made biopharmaceutical by FDA for human use, many protein drugs are now in clinical development. Within the last decade, significant advances have been made in expression of heterologous complex/large proteins in chloroplasts of edible plants using codon optimized human or viral genes. Furthermore, advances in quantification enable determination of in-planta drug dosage. Oral delivery of plastid-made biopharmaceuticals (PMB) is affordable because it eliminates prohibitively expensive fermentation, purification processes addressing major challenges of short shelf-life after cold storage...
February 21, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28229383/mirna-based-heavy-metal-homeostasis-and-plant-growth
#11
Ali Noman, Muhammad Aqeel
Plants have been naturally gifted with mechanisms to adjust under very high or low nutrient concentrations. Heavy metal toxicity is considered as a major growth and yield-limiting factor for plants. This stress includes essential as well as non-essential metals. MicroRNAs (miRNAs) are known for mediating post-transcriptional regulation by cleaving transcripts or translational inhibition. It is commonly agreed that an extensive understanding of plant miRNAs will significantly help in the induction of tolerance against environmental stresses...
February 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28229269/in-arabidopsis-thaliana-distinct-alleles-encoding-mitochondrial-rna-processing-factor-4-support-the-generation-of-additional-5-termini-of-ccmb-transcripts
#12
Katrin Stoll, Christian Jonietz, Sarah Schleicher, Catherine Colas des Francs-Small, Ian Small, Stefan Binder
In plant mitochondria, the 5' ends of many transcripts are generated post-transcriptionally. We show that the pentatricopeptide repeat (PPR) protein RNA PROCESSING FACTOR 4 (RPF4) supports the generation of extra 5' ends of ccmB transcripts in Landsberg erecta (Ler) and a number of other Arabidopsis thaliana ecotypes. RPF4 was identified in Ler applying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which generates the Ler-type extra ccmB 5' termini only after the introduction of the RPF4 allele from Ler...
February 22, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28229068/the-characteristics-of-emotional-response-of-post-traumatic-stress-disorder-and-post-traumatic-growth-among-chinese-adults-exposed-to-an-explosion-incident
#13
Chuguang Wei, Jin Han, Yuqing Zhang, Walter Hannak, Zhengkui Liu
PURPOSE: Post-traumatic stress disorder (PTSD) and post-traumatic growth (PTG) are two different outcomes that may occur after experiencing traumatic events. Meanwhile, the traumatic exposure level and emotion response played an important role in the process. The present study first evaluated the relationship between PTSD, PTG, and traumatic exposure level and then compared the characteristics of emotional response through response time of the affective priming paradigm. METHODS: For the purpose of evaluating the relationship between PTSD, PTG, and trauma exposure level, a sample of 2,395 participants completed measures of posttraumatic stress disorder Checklist-Civilian Version (PCL-C), Post-traumatic Growth Inventory (PTGI) and a trauma exposure-related survey, and Pearson's correlation analysis for the scales were conducted...
2017: Frontiers in Public Health
https://www.readbyqxmd.com/read/28228127/a-review-of-enhanced-paramedic-roles-during-and-after-hospital-handover-of-stroke-myocardial-infarction-and-trauma-patients
#14
Darren Flynn, Richard Francis, Shannon Robalino, Joanne Lally, Helen Snooks, Helen Rodgers, Graham McClelland, Gary A Ford, Christopher Price
BACKGROUND: Ambulance paramedics play a critical role expediting patient access to emergency treatments. Standardised handover communication frameworks have led to improvements in accuracy and speed of information transfer but their impact upon time-critical scenarios is unclear. Patient outcomes might be improved by paramedics staying for a limited time after handover to assist with shared patient care. We aimed to categorize and synthesise data from studies describing development/extension of the ambulance-based paramedic role during and after handover for time-critical conditions (trauma, stroke and myocardial infarction)...
February 23, 2017: BMC Emergency Medicine
https://www.readbyqxmd.com/read/28228043/impact-of-exercise-on-clinical-symptom-report-and-neurocognition-following-concussion-in-children-and-adolescents
#15
Vicky Manikas, Franz Babl, Stephen J C Hearps, Julian Dooley, Vicki Anderson
Recovery from concussion in childhood is poorly understood, despite its importance in decisions regarding return to normal activity. Resolution of post-concussive symptoms (PCS) is widely employed as a marker of recovery in clinical practice, however it is unclear if subtle impairments persist only to re-emerge in the context of increased physical or cognitive demands. This study aimed to examine the effect of strenuous exercise on clinical symptom report and neurocognition in children and adolescents after PCS resolution following concussion We recruited concussed children and adolescents (n=30) on presentation to an Emergency Department (ED)...
February 22, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28228002/custom-shaped-organic-photovoltaic-modules-freedom-of-design-by-printing
#16
M Välimäki, E Jansson, P Korhonen, A Peltoniemi, S Rousu
Freedom of design that was introduced as organic photovoltaic (OPV) modules were fabricated by printing. As proof-of-concept, we show OPV leaf fabrication in A5 size using gravure and rotary screen printing processes for the main active layers of the OPV structure. These printing methods allow direct printing of any kind of arbitrary, two-dimensional shapes including patterning of the electric contacts thus post-patterning stages are not needed. Fabrication of custom-shaped OPV modules requires detailed information about the technical boundaries set by the manufacturing process and materials which in turn influence the layout design and R2R upscaling...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28227911/an-implementation-for-the-simulation-of-cells-on-micro-post-arrays
#17
Duy T Truong, Christian Bahls, Barbara Nebe, Ursula van Rienen, Duy T Truong, Christian Bahls, Barbara Nebe, Ursula van Rienen, Duy T Truong, Christian Bahls, Ursula van Rienen, Barbara Nebe
The mechanical interaction between cells and their underlying substrates is important in understanding the processes that take place at an interface between biological tissue and the surface of implants. There have been numerous studies that examine these interactions both by experimental and numerical modeling. The bio-chemo-mechanical model for cell contractility by Deshpande et al. [1] has numerous applications and advantages. This work shows a way to implement this model in COMSOL MULTIPHYSICS® so it can be easily modified or extended...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227782/predicting-short-term-icu-outcomes-using-a-sequential-contrast-motif-based-classification-framework
#18
Shameek Ghosh, Hung Nguyen, Jinyan Li, Shameek Ghosh, Hung Nguyen, Jinyan Li, Shameek Ghosh, Hung Nguyen, Jinyan Li
Critical ICU events like acute hypotension and septic shock are dangerous complications, leading to multiple organ failures and eventual death. Previously, pattern mining algorithms have been employed for extracting interesting rules in various clinical domains. However, the extracted rules are directly investigated by clinicians for diagnosing a disease. Towards this purpose, there is a need to develop advanced prediction models which integrate dynamic patterns to learn a patient's physiological condition...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227747/split-belt-adaptation-and-gait-symmetry-in-transtibial-amputees-walking-with-a-hybrid-emg-controlled-ankle-foot-prosthesis
#19
Oliver A Kannape, Hugh M Herr, Oliver A Kannape, Hugh M Herr, Oliver A Kannape, Hugh M Herr
Our ability to automatically adapt our walking pattern to the demands of our environment is central to maintaining a steady gait. Accordingly, a large effort is being made to extend and integrate this adaptability to lower-limb prostheses. To date, the main focus of this research has been on short term adaptation, such as in response to a terrain transition or a sudden change in the environment. However, long term adaptation and underlying sensorimotor learning processes are critical to optimizing walking patterns and predictively changing our gait when faced with continued perturbations...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227647/a-feasibility-study-of-depth-image-based-intent-recognition-for-lower-limb-prostheses
#20
Huseyin Atakan Varol, Yerzhan Massalin, Huseyin Atakan Varol, Yerzhan Massalin, Huseyin Atakan Varol, Yerzhan Massalin
This paper presents our preliminary work on a depth camera based intent recognition system intended for future use in robotic prosthetic legs. The approach infers the activity mode of the subject for standing, walking, running, stair ascent and stair descent modes only using data from the depth camera. Depth difference images are also used to increase the performance of the approach by discriminating between static and dynamic instances. After confidence map based filtering, simple features such as mean, maximum, minimum and standard deviation are extracted from rectangular regions of the frames...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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