keyword
MENU ▼
Read by QxMD icon Read
search

hacat

keyword
https://www.readbyqxmd.com/read/28745083/elemental-diet-accelerates-the-recovery-from-oral-mucositis-and-dermatitis-induced-by-5-fluorouracil-through-the-induction-of-fibroblast-growth-factor-2
#1
Koji Harada, Tarannum Ferdous, Hiroaki Kobayashi, Yoshiya Ueyama
Mucositis and dermatitis induced by anticancer agents are common complications of anticancer therapies. In this study, we evaluated the efficacy of Elental (Ajinomoto Pharmaceutical Ltd, Tokyo, Japan), an elemental diet with glutamine in the treatment of 5-fluorouracil (5-FU)-induced oral mucositis and dermatitis in vivo and tried to clarify the underlying mechanisms of its action. Oral mucositis and dermatitis was induced through a combination of 5-FU treatment and mild abrasion of the cheek pouch in hamsters and the dorsal skin in nude mice respectively...
July 1, 2017: Integrative Cancer Therapies
https://www.readbyqxmd.com/read/28736282/imiquimod-induced-psoriasis-like-inflammation-in-differentiated-human-keratinocytes-its-evaluation-using-curcumin
#2
Sandeep R Varma, Thiyagarajan O Sivaprakasam, Abheepsa Mishra, Sunil Prabhu, Rafiq M, Rangesh P
Psoriasis is considered to be a systemic disease of immune dysfunction. It is still unclear what triggers the inflammatory cascade associated with psoriasis but recent evidences suggest the vital role of IL-23/IL-17A cytokine axis in etiology of psoriasis. Several studies have been conducted in psoriatic-like animal models but ethical issues and complexity surrounding it halts the screening of new anti-psoriatic drug candidates. Hence, in this study, we developed a new in-vitro model for psoriasis using imiquimod (IMQ) induced differentiated HaCaT cells which could be used for screening of new anti-psoriatic drug candidates...
July 20, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28734664/ph-responsive-biodegradable-nanogels-for-sustained-release-of-bleomycin
#3
Prashant Sahu, Sushil K Kashaw, Varun Kushwah, Samaresh Sau, Sanyog Jain, Arun K Iyer
Site specific drug delivery with desired therapeutic effect still remains challenging task due to suboptimal release, tissue toxicity, low selectivity and meager therapeutic efficacy in skin cancers. The aim of the current study was to fabricate pH responsive, self-assembled, chemically cross-linked biodegradable chitosan nanogel loaded with bleomycin to target the dermal area of the skin. The nanogel synthesized by ion gelation technique and was characterized for drug loading, swelling and thermal stability followed by in vitro analysis...
June 27, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28733577/skin-renewal-activity-of-non-thermal-plasma-through-the-activation-of-%C3%AE-catenin-in-keratinocytes
#4
J H Choi, Y S Song, K Song, H J Lee, J W Hong, G C Kim
For recent years, devices that generate non-thermal plasma (NTP) have been introduced into the field of dermatology. Since NTP has demonstrated strong anti-pathogenic activity with safety of use, NTP was first applied to sterilize the skin surface to aid in the healing of various kinds of skin diseases. However, the effect of NTP on skin regeneration has not yet been fully explored. In this study, the effect of NTP on the growth of keratinocytes was tested using the HaCaT human keratinocyte cell line and HRM2 hairless mice...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28732404/influence-of-the-stabilizers-on-the-toxicity-of-metallic-nanomaterials-in-aquatic-organisms-and-human-cell-lines
#5
Tatiana Andreani, Verónica Nogueira, Vera V Pinto, Maria José Ferreira, Maria Graça Rasteiro, Amélia M Silva, Ruth Pereira, Carlos M Pereira
In this study, following a systematic approach, we used aquatic species (bacteria Vibrio fischeri and microalgae Raphidocelis subcapitata) and different human cell lines (Caco-2, HepG2, SV-80 and HaCaT) representing different tissues and exposure pathways, to investigate how two organic stabilizers (PVA and DMSO) used for NMs dispersion influence their physicochemical properties, the persistence of metals in suspension and the toxicity/ecotoxicity of two metallic NMs (nano-Ag and nano-Cu). Although the stabilizers are expected to contribute to improve the dispersion and stability of NMs, the results obtained clearly showed that no similar changes in toxicity and morphological properties of the nano-Ag can be expected after its stabilization with PVA...
July 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28731282/altered-signaling-associated-with-chronic-arsenic-exposure-in-human-skin-keratinocytes
#6
Sartaj Ahmad Mir, Santosh Renuse, Gajanan Sathe, Aafaque Ahmad Khan, Arun H Patil, Vishalakshi Nanjappa, Firdous Ahmad Bhat, T S Keshava Prasad, Ashok K Giri, Aditi Chatterjee, Harsha Gowda
Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here we carried out SILAC-based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4,171 unique phosphosites derived from 2,000 proteins. We observed differential phosphorylation of 406 phosphosites (2-fold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organization were found to be significantly enriched (p<0...
July 21, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28730954/evaluation-of-in-vitro-anti-psoriatic-activity-of-a-novel-polyherbal-formulation-by-multiparametric-analysis
#7
Nadav Shraibom, Alka Madaan, Vidushi Joshi, Ritu Verma, Anika Chaudhary, Gautam Mishra, Anshumali Awasthi, Anu Taneja Singh, Manu Jaggi
The beneficial role of traditional herbal medicines in the treatment of inflammatory skin disorders has been widely reported. Psoriasis is characterized by uncontrolled hyper-proliferation of keratinocytes, disturbed apoptosis, over-secretion of inflammatory cytokines and angiogenic factors. We have developed a novel aqueous polyherbal formulation (SIRB-001) consisting of 3 herbs; Rheum palmatum L., Rehmannia glutinosa Libosch and Lonicera Japonica in the ratio 1:1:3. SIRB-001 has demonstrated efficacious effects in psoriasis patients...
July 20, 2017: Anti-inflammatory & Anti-allergy Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28728908/the-proteasome-maturation-protein-pomp-increases-proteasome-assembly-and-activity-in-psoriatic-lesional-skin
#8
Barbara A Zieba, Laurent Henry, Matthieu Lacroix, Mohamed Jemaà, Thierry Lavabre-Bertrand, Laurent Meunier, Olivier Coux, Pierre-Emmanuel Stoebner
BACKGROUND: The ubiquitin proteasome pathway is involved in the pathogenesis of psoriasis and proteasome subunits are increased in lesional psoriatic skin. Recent works have highlighted that proteasome levels can be regulated through modulation of proteasome assembly notably by the proteasome maturation protein POMP. OBJECTIVES: To investigate whether proteasome assembly and POMP expression are modified in psoriatic skin. METHODS: Proteasome assembly as well as expression of proteasome regulators were assessed in non-lesional and lesional psoriatic skin using native gel electrophoresis and western blots respectively...
April 30, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28726210/sodium-dodecyl-sulfate-cytotoxicity-towards-hacat-keratinocytes-comparative-analysis-of-methods-for-evaluation-of-cell-viability
#9
A L Rusanov, N G Luzgina, A V Lisitsa
Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of viability under the effect of sodium dodecyl sulfate, proportional to the length of exposure. The values measured by different methods (MTT test and neutral red absorption test) varied, the differences were determined by the duration of exposure to sodium dodecyl sulfate...
June 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28721664/reciprocal-influence-of-hmscs-hacat-cultivated-on-electrospun-scaffolds
#10
Sirsendu Bhowmick, Sandra Rother, Heike Zimmermann, Poh S Lee, Stephanie Moeller, Matthias Schnabelrauch, Veena Koul, Dieter Scharnweber
Here, we investigated the synergistic effect of electrospun nanofibrous scaffolds made of gelatin /sulfated hyaluronan (sHA) or native hyaluronan (HA)/chondroitin sulfate (CS) and, keratinocytes (HaCaT)-human mesenchymal stem cells (hMSCs) contact co-culture on epithelial differentiation of hMSCs. The hMSCs were co-cultured in contact with HaCaT cells for 5 days on electrospun scaffold. Results show that electrospun scaffolds containing sulfated glycosaminoglycans (GAGs) stimulate epithelial differentiation in terms of various protein expression markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of several dermal proteins (keratin 14, ΔNp63α)...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28717410/fish-scale-collagen-peptides-protect-against-cocl2-tnf-%C3%AE-induced-cytotoxicity-and-inflammation-via-inhibition-of-ros-mapk-and-nf-%C3%AE%C2%BAb-pathways-in-hacat-cells
#11
Fazli Subhan, Hae Yeong Kang, Yeseon Lim, Muhammad Ikram, Sun-Yong Baek, Songwan Jin, Young Hun Jeong, Jong Young Kwak, Sik Yoon
Skin diseases associated with inflammation or oxidative stress represent the most common problem in dermatology. The present study demonstrates that fish scale collagen peptides (FSCP) protect against CoCl2-induced cytotoxicity and TNF-α-induced inflammatory responses in human HaCaT keratinocyte cells. Our study is the first to report that FSCP increase cell viability and ameliorate oxidative injury in HaCaT cells through mechanisms mediated by the downregulation of key proinflammatory cytokines, namely, TNF-α, IL-1β, IL-8, and iNOS...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28716547/cellular-senescence-regulated-by-swi-snf-complex-subunits-through-p53-p21-and-p16-prb-pathway
#12
Ling He, Ying Chen, Jianguo Feng, Weichao Sun, Shun Li, Mengting Ou, Liling Tang
SWI/SNF complex is an evolutionarily well-conserved chromatin-remodeling complex, which is implicated in the nucleosomes removing or sliding, impacting on the DNA repair, replication and genes expression regulation. The SWI/SNF complex consists up to 12 protein subunits. The catalytic subunits are BRG1 or BRM, which are exclusive ATPase subunits. BRG1 has been reported to play an important role in cellular senescence. However, The function of non-catalytic subunits involved in cellular senescence is rarely investigated...
July 14, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28713942/ginsenoside-rc-protects-against-uvb%C3%A2-induced-photooxidative-damage-in-epidermal-keratinocytes
#13
Yuri Oh, Hye-Won Lim, Kwang Hark Park, Yu-Hua Huang, Ji-Young Yoon, Kyunghoon Kim, Chang-Jin Lim
Ginsenoside Rc (Rc) is a major ginsenoside isolated from Panax ginseng, and has exhibited pharmacological effects on skin cells. The present study aimed to investigate the putative skin‑protective properties of Rc, including its anti‑photoaging and barrier function‑protective effects, in human HaCaT keratinocytes exposed to UVB radiation. The protective properties of Rc were evaluated through the assessment of keratinocyte viability, reactive oxygen species (ROS) production, total glutathione (GSH) and superoxide dismutase (SOD) activity, caspase‑14, matrix metalloproteinase (MMP)‑2 and ‑9 activity, and MMP‑2, MMP‑9 and filament aggregating protein (filaggrin) expression following UVB irradiation...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713016/a-conducive-bioceramic-polymer-composite-biomaterial-for-diabetic-wound-healing
#14
Fang Lv, Jie Wang, Peng Xu, Yiming Han, Hongshi Ma, He Xu, Shijie Chen, Jiang Chang, Qinfei Ke, Mingyao Liu, Zhengfang Yi, Chengtie Wu
Diabetic wound is a common complication of diabetes. Biomaterials offer great promise in inducing tissue regeneration for chronic wound healing. Herein, we reported a conducive Poly (caprolactone) (PCL)/gelatin nanofibrous composite scaffold containing silicate-based bioceramic particles (Nagelschmidtite, NAGEL, Ca7P2Si2O16) for diabetic wound healing. NAGEL bioceramic particles were well distributed in the inner of PCL/gelatin nanofibers via co-electrospinning process and the Si ions maintained a sustained release from the composite scaffolds during the degradation process...
July 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28711618/fabrication-of-bi-layer-scaffold-of-keratin-nanofiber-and-gelatin-methacrylate-hydrogel-implications-for-skin-graft
#15
Jong Wook Kim, Min Jin Kim, Chang Seok Ki, Hyun Jeong Kim, Young Hwan Park
Bi-layer scaffold composed of human hair keratin/chitosan nanofiber mat and gelatin methacrylate (GelMA) hydrogel was fabricated by using electrospinning and photopolymerization techniques. To prepare the nanofiber layer, the blend solution of human hair keratin and chitosan (mixture ratio: 5/5) was electrospun using formic acid as a solvent in the presence of poly(ethylene glycol), followed by cross-linking with glutaraldehyde. The tensile strength of the human hair keratin/chitosan nanofiber mat was much higher than that of pure human hair keratin nanofiber mat...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28711022/pre-clinical-efficacy-assessment-of-malva-sylvestris-on-chronic-skin-inflammation
#16
Arthur S Prudente, Graziela Sponchiado, Daniel A G B Mendes, Bruna S Soley, Daniela A Cabrini, Michel F Otuki
In the search for improved quality of life, the treatment of skin diseases like psoriasis (hyperproliferative disease) is valid, since it causes huge social discomfort to the patient. In this context, earlier studies showed that Malva sylvestris L. has anti-inflammatory activity demonstrated by acute animal models of skin inflammation, becoming a promising target for further studies. The present investigation aimed to verify the effect of hydroalcoholic extract of M. sylvestris (HEMS) on the chronic inflammatory and hyperproliferative response caused by multiple applications of 12-O-tetradecanoylphorbol-13-acetate (TPA) on mouse ears...
July 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28704964/myrcia-splendens-sw-dc-syn-m-fallax-rich-dc-myrtaceae-essential-oil-from-amazonian-ecuador-a-chemical-characterization-and-bioactivity-profile
#17
Laura Scalvenzi, Alessandro Grandini, Antonella Spagnoletti, Massimo Tacchini, David Neill, José Luis Ballesteros, Gianni Sacchetti, Alessandra Guerrini
In this study, we performed the chemical characterization of Myrcia splendens (Sw.) DC. (Myrtaceae) essential oil from Amazonian Ecuador and the assessment of its bioactivity in terms of cytotoxic, antibacterial, and antioxidant activity as starting point for possible applicative uses. M. splendens essential oil, obtained by hydro-distillation, was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID): the major components were found to be trans-nerolidol (67...
July 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28700975/astilbin-decreases-proliferation-and-improves-differentiation-in-hacat-keratinocytes
#18
Chunhong Zhang, Qingqing Xu, Xi Tan, Liya Meng, Guo Wei, Ying Liu, Chunmin Zhang
Psoriasis is a common chronic dermatosis characterized by keratinocyte hyperproliferation accompanied by inflammatory reactions. Pathological changes upset the balance between keratinocyte proliferation, differentiation, and death in psoriatic lesions, suggesting that molecules with topical anti-inflammatory, anti-proliferation and anti-angiogenesis abilities may be useful for its treatment. The flavonoid astilbin is the major active component extracted from the rhizome of Smilax glabra, which has been widely used in China to treat inflammatory and autoimmune diseases...
July 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28694748/protective-effects-of-scutellaria-baicalensis-georgi-against-hydrogen-peroxide-induced-dna-damage-and-apoptosis-in-hacat-human-skin-keratinocytes
#19
Jung Jeh Yoon, Jin-Woo Jeong, Eun Ok Choi, Min Ju Kim, Hyun Hwang-Bo, Hong Jae Kim, Su Hyun Hong, Cheol Park, Dong Hee Lee, Yung Hyun Choi
Oxidative stress due to excessive accumulation of reactive oxygen species (ROS) is one of the risk factors for the development of several chronic diseases. In this study, we investigated the protective effects of Scutellaria baicalensis rhizome ethanol extract (SBRE) against oxidative stress-induced cellular damage and elucidated the underlying mechanisms in the HaCaT human skin keratinocyte cell line. Our results revealed that treatment with SBRE prior to hydrogen peroxide (H2O2) exposure significantly increased viability of HaCaT cells...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28691693/influence-of-physico-mechanical-properties-of-elastomeric-material-for-different-cell-growth
#20
Jyoti Wala, Debashis Maji, Soumen Das
The tunable mechanical and physical properties of Polydimethylsiloxane (PDMS) are commonly utilized for studying cellular dynamics. However, the inherent hydrophobic nature of PDMS limits its application as cell culture films. Various surface modification techniques render PDMS films hydrophilic which alters the surface chemistry, elasticity, roughness and cell attachment of anchorage dependent cell types to the films. The surface properties of thin film lead to alteration in bio-mechano-physical properties of cells and therefore could be used as mechanical signature for viability testing of different normal and cancerous cells...
July 10, 2017: Biomedical Materials
keyword
keyword
79429
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"