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https://www.readbyqxmd.com/read/29778747/novel-peptide-inhibits-inflammation-by-suppressing-of-protease-activated-receptor-2
#1
Bora Kim, Hyun-Soo Kim
We synthesized and investigated the effects of a novel peptide B, RRFSLLRY as a novel PAR-2 antagonist. Peptide B decreased calcium levels in HaCat cells stimulated with trypsin and PAR-2 activator (SLIGKV) and cytokeratin 14 and PCNA was decreased by peptide B. Peptides B also reduced the expression of inflammatory cytokines such as TNF-α and interleukin-6. Significant increase of oxazolone-induced transepidermal water loss (TEWL) was decreased by the addition of peptide B in hairless mice. These findings suggest that the anti-inflammatory effect of peptide B is due to inhibition of PAR-2 signaling...
May 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29767264/elemental-diet-inhibits-pro%C3%A2-inflammatory-cytokine-production-in-keratinocytes-through-the-suppression-of-nf%C3%A2-%C3%AE%C2%BAb-activation
#2
Koji Harada, Tarannum Ferdous, Yoichi Mizukami, Katsuaki Mishima
An elemental diet (ED) has been reported to reduce oral mucositis and dermatitis induced by chemotherapy. However, its molecular mechanism of action as an anti‑inflammatory agent is still unknown. The aim of the present study was to clarify whether ED confers its anti‑inflammatory action via reduction of pro‑inflammatory cytokine production in keratinocytes in vivo and in vitro. We evaluated the efficacy of ED in the treatment of 5‑fluorouracil (5‑FU)‑induced dermatitis of nude mice, and examined the expression of pro‑inflammatory cytokines such as tumor necrosis factor‑α (TNF‑α), interleukin (IL)‑1β and IL‑6 using immunohistochemistry...
May 16, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29767259/silencing-of-mir%C3%A2-155-suppresses-inflammatory-responses-in-psoriasis-through-inflammasome-nlrp3-regulation
#3
Quan Luo, Jingxin Zeng, Wei Li, Ling Lin, Xin Zhou, Xin Tian, Weiyu Liu, Lidan Zhang, Xibao Zhang
Psoriasis is a dermatosis with the major clinical symptoms of scale, erythema and itching, and it has a long disease course. In addition, it is easily recurrent and refractory, greatly affecting the physical and mental health of patients. In the present study, it was hypothesized that the function of miR‑155 increases psoriasis‑induced inflammation and that its expression may be dependent on inflammasome activation. miR‑155 expression was examined by gene chip array and quantitative polymerase chain reaction analysis...
May 14, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29763495/early-response-of-fibroblasts-and-epithelial-cells-to-pink-shaded-anodized-dental-implant-abutments-an-in-vitro-study
#4
Federico Mussano, Tullio Genova, Marco Laurenti, Elisa Zicola, Luca Munaron, Paola Rivolo, Pietro Mandracci, Stefano Carossa
PURPOSE: This research aimed to assess whether pink-shaded anodized surfaces could enhance the adhesion of soft tissue cells compared with untreated machined titanium surfaces. MATERIALS AND METHODS: Two types of Ti-Al-V titanium samples were prepared: machined titanium (Ti) and anodized titanium (AnoTi). The microstructure was studied by means of a scanning electron microscope. X-ray photoelectron spectroscopy (XPS) was carried out as well. The wetting properties were investigated by the sessile drop technique with water and diiodomethane...
May 2018: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29757659/bone-marrow-derived-mesenchymal-stem-cells-promoted-cutaneous-wound-healing-by-regulating-keratinocyte-migration-via-the-%C3%AE-2-adrenergic-receptor-signaling
#5
Jiahui Huo, Sujing Sun, Zhijun Geng, Wei Sheng, Runkai Chen, Kui Ma, Xiaoyan Sun, Xiaobing Fu
Mesenchymal stem cells (MSCs) play an important role in the cutaneous wound healing; however, the functional mechanisms involved in the healing process are poorly understood. A series of studies indicate that keratinocytes which migrate into the wound bed rely on an epithelial-mesenchymal transition (EMT)-like process to initiate re-epithelialization. We therefore examined whether bone marrow-derived MSCs (BMSCs) could affect biological behavior and induce EMT-like characteristics in the human epidermal keratinocytes (HEKs) and in the immortalized human keratinocyte cell line HaCaT cells; and we investigated the signaling pathways of BMSC-mediated phenotypic changes...
May 14, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29755556/defensive-properties-of-ginsenoside-re-against-uv-b-induced-oxidative-stress-through-up-regulating-glutathione-and-superoxide-dismutase-in-hacat-keratinocytes
#6
Daehyun Shin, Hee Won Moon, Yuri Oh, Kyunghoon Kim, Dae-Duk Kim, Chang-Jin Lim
Ginseng is now used worldwide as a traditional Oriental medicine. Ginsenosides, also known as ginseng saponins, are responsible for most pharmacological efficacies of ginseng. This work aimed to assess the novel skin anti-photoaging potential of ginsenoside Re (Re), a protopanaxatriol-type ginsenoside, by analyzing reactive oxygen species (ROS), pro-matrix metalloproteinase-2 (proMMP-2) and -9 (proMMP-9), total glutathione (GSH), superoxide dismutase (SOD), and cellular viability in UV-B-irradiated HaCaT keratinocytes...
2018: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/29750792/epiisopilosine-alkaloid-has-activity-against-schistosoma-mansoni-in-mice-without-acute-toxicity
#7
Maria A Guimarães, Rosimeire N de Oliveira, Rebeca L de Almeida, Ana C Mafud, Ana L V Sarkis, Rayane Ganassin, Marcos P da Silva, Daniel B Roquini, Leiz M Veras, Tânia C H Sawada, Cristina D Ropke, Luis A Muehlmann, Graziella A Joanitti, Selma A S Kuckelhaus, Silmara M Allegretti, Yvonne P Mascarenhas, Josué de Moraes, José R S A Leite
Schistosomiasis is a disease caused by parasites of the genus Schistosoma, currently affecting more than 200 million people. Among the various species of this parasite that infect humans, S. mansoni is the most common. Pharmacological treatment is limited to the use of a single drug, praziquantel (PZQ), despite reports of parasite resistance and low efficacy. It is therefore necessary to investigate new potential schistosomicidal compounds. In this study, we tested the efficacy of epiisopilosine (EPIIS) in a murine model of schistosomiasis...
2018: PloS One
https://www.readbyqxmd.com/read/29750291/collagen-peptides-from-soft%C3%A2-shelled-turtle-induce-calpain%C3%A2-1-expression-and-regulate-inflammatory-cytokine-expression-in-hacat-human-skin-keratinocytes
#8
Tetsushi Yamamoto, Saori Nakanishi, Kuniko Mitamura, Atsushi Taga
Collagen peptides (CPs), derived by hydrolyzing collagen with chemicals or enzymes, are often used as functional materials, due to their various bioactivities and high bioavailability. A previous study by our group reported that collagen from soft‑shelled turtle, Pelodiscus sinensis, induces keratinocytes to undergo epithelial‑mesenchymal transition and facilitates wound healing. Therefore, CPs derived from soft‑shelled turtle collagen may have useful effects on the skin. In the present study, the functional effects of CPs on human skin were examined by analyzing CP‑treated human keratinocytes with a shotgun liquid chromatography/mass spectrometry‑based global proteomic approach...
May 8, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29748001/nfkb1-mediates-th1-th17-activation-in-the-pathogenesis-of-psoriasis
#9
Fusheng Zhou, Zhengwei Zhu, Jinping Gao, Chao Yang, Leilei Wen, Lu Liu, Xianbo Zuo, Xiaodong Zheng, Yinjuan Shi, Caihong Zhu, Bo Liang, Xianyong Yin, Wenjun Wang, Hui Cheng, Songke Shen, Xianfa Tang, Huayang Tang, Liangdan Sun, Anping Zhang, Sen Yang, Xuejun Zhang, Yujun Sheng
This study was aimed to investigate whether NFKB1 participates in the pathogenesis of psoriasis by mediating Th1/Th17 cells. In this study, expression of NFKB1 was assessed in skin tissues from psoriasis patients and the healthy controls through Western blot and Immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the serum levels of IFN-γ, IL-17 (IL-17A) and IL-17RA. The imiquimod-induced psoriasis mouse model was employed to examine the role of NFKB1 in psoriasis via the assessment of psoriasis area and severity index (PASI), including erythema, thickness and scales...
May 2, 2018: Cellular Immunology
https://www.readbyqxmd.com/read/29737589/ll-37-fragments-have-antimicrobial-activity-against-staphylococcus-epidermidis-biofilms-and-wound-healing-potential-in-hacat-cell-line
#10
Paola Saporito, Michelle Vang Mouritzen, Anders Løbner-Olesen, Håvard Jenssen
Staphylococcus epidermidis is a common nosocomial pathogen able to form biofilms in indwelling devices, resulting in chronic infections, which are refractory to antibiotics treatment. Staphylococcal biofilms are also associated with the delayed reepithelization and healing of chronic wounds. The human cathelicidin peptide LL-37 has been proven active against S. epidermidis biofilms in vitro and to promote wound healing. As previous studies have demonstrated that fragments of LL-37 could possess an equal antibacterial activity as the parent peptide, we tested whether shorter (12-mer) synthetic fragments of LL-37 maintained the antibiofilm and/or immune modulating activity, aiming at the identification of essential regions within the LL-37 parent sequence...
May 8, 2018: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/29736291/optimization-of-cytokine-milieu-to-reproduce-atopic-dermatitis-related-gene-expression-in-hacat-keratinocyte-cell-line
#11
Hee Joo Kim, Jinok Baek, Jong Rok Lee, Joo Young Roh, YunJae Jung
Although atopic dermatitis (AD) is characterized by cytokine production predominantly mediated by T helper (Th) 2 cells, AD pathogenesis also involves innate immune and Th1 cells. To optimize the cytokine milieu required for accurate reproduction of AD-related gene expression profile in vitro , we evaluated the expression pattern of CCL22 , CCL17 , IL5 , IL13 , IL33 , IL25 , TSLP , FLG , and LOR in human lesional AD skin and cytokine-stimulated HaCaT cells. An increase in Th2 mediators ( IL5 , IL13 , CCL22 , CCL17, IL25 , IL33 , and TSLP ) and a decrease in genes related to cornified cell envelope (filaggrin and loricrin) were observed in human AD lesions...
April 2018: Immune Network
https://www.readbyqxmd.com/read/29726712/the-medicinal-mushroom-auricularia-auricula-judae-bull-extract-has-antioxidant-activity-and-promotes-procollagen-biosynthesis-in-hacat-cells
#12
Young-Ji Choi, In-Sun Park, Mi-Hee Kim, Bora Kwon, Young-Moo Choo, Seung-Il Jeong, Kang-Yeol Yu, Chung Hyeon Choe, Jiyoung Kim
In this study, Auricularia auricula-judae (Bull.) extract (AAE) had potent antioxidant activity in vitro and promoted the biosynthesis of procollagen, a precursor of collagen in HaCaT cells. In addition, the expression of HAS-3 (hyaluronic acid synthase), which is a moisturizing factor, was increased in HaCaT cells in response to AAE. Therefore, this work suggests that AAE has the potential to exhibit antioxidant activity and promote procollagen biosynthesis in HaCaT cells.
May 4, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29719471/synergistic-effect-of-maclurin-on-ginsenoside-compound-k-induced-inhibition-of-the-transcriptional-expression-of-matrix-metalloproteinase-1-in-hacat-human-keratinocyte-cells
#13
https://www.readbyqxmd.com/read/29719290/identification-of-a-novel-keratin-9-missense-mutation-in-a-chinese-family-with-epidermolytic-palmoplantar-keratoderma
#14
Heng Xiao, Yi Guo, Junhui Yi, Hong Xia, Hongbo Xu, Lamei Yuan, Pengzhi Hu, Zhijian Yang, Zhenghao He, Hongwei Lu, Hao Deng
BACKGROUND/AIMS: Epidermolytic palmoplantar keratoderma (EPPK) is an autosomal dominant genodermatosis. It is characterized by diffuse yellow keratoses on the palmoplantar epidermis, with an erythematous border. The keratin 9 gene (KRT9) and less frequently the keratin 1 gene (KRT1) are responsible for EPPK. This study aims to identify and analyse genetic defects responsible for EPPK in a Han Chinese pedigree. METHODS: A four-generation Han Chinese pedigree containing five individuals affected with EPPK was recruited...
April 26, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29714641/in-vitro-characterization-of-cutaneous-immunotoxicity-of-immortalized-human-keratinocytes-hacat-exposed-to-reactive-and-disperse-textile-dyes
#15
Daniela Morais Leme, Andrea Sehr, Tamara Grummt, Jenifer Pendiuk Gonçalves, Thiago Jacomasso, Sheila Maria Brochado Winnischofer, Francine Bittencourt Potrich, Carolina Camargo de Oliveira, Edvaldo da Silva Trindade, Danielle Palma de Oliveira
Several synthetic dyes are used by textile industry for supplying the market of colored clothes. However, these chemicals have been associated with a variety of adverse human health effects, including textile dermatitis. Thus, there is a growing concern to identify textile dyes potentially as skin immunotoxicants. The aim of this in vitro study was to characterize the immunotoxic potential of reactive (Reactive Green 19 [RG19], Reactive Blue 2 [RB2], Reactive Black 5 [RB5]) and disperse (Disperse Red 1 [DR1]) textile dyes using a dermal cell line...
May 1, 2018: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/29709911/sphingoid-base-upregulated-caspase-14-expression-involves-mapk
#16
Yukitoshi Nagahara, Kei Kawakami, Abudubari Sikandan, Daiki Yagi, Ryo Nishikawa, Takahisa Shinomiya
Sphingolipids are putative intracellular signal mediators in cell differentiation, growth inhibition, and apoptosis. Especially, sphingoid base-backbones of sphingolipids (sphingosine, sphinganine, and phytosphingosine) and their metabolites N-acyl-sphingoid bases (ceramides) are highly bioactive. In skin, one of the caspases, caspase-14, is expressed predominantly in cornifying epithelia, and caspase-14 plays an important role in keratinocyte differentiation. As ceramides were surrounding lipids in the keratinocytes and ceramides stimulate keratinocyte differentiation, we therefore examined the upregulation of caspase-14 by various sphingoid bases and ceramide...
2018: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29704506/resveratrol-alleviates-lps-induced-injury-in-human-keratinocyte-cell-line-hacat-by-up-regulation-of-mir-17
#17
Xiuchun Wang, Yali Zhang
BACKGROUND: Resveratrol (RSV), an edible polyphenolic phytoalexin, plays an important role in ameliorating inflammation, including skin inflammation after burn injury. However, the specific molecular mechanism underlying its anti-inflammation effect is still unclear. Herein, the effect and the mechanism underlying the protection of HaCaT cells by RSV against inflammation were examined. METHODS: Lipopolysaccharide (LPS)-induced inflammation and the cytoprotection of RSV were evaluated by detecting viability, apoptosis, expressions of apoptosis-associated proteins and the productions of pro-inflammatory factors by CCK-8 assay, flow cytometer, Western blot, and qRT-PCR...
April 25, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29702749/melatonin-and-its-derivatives-counteract-the-ultraviolet-b-radiation-induced-damage-in-human-and-porcine-skin-ex-vivo
#18
Cezary Skobowiat, Anna A Brożyna, Zorica Janjetovic, Jeayeng Saowanee, Allen S W Oak, Tae-Kang Kim, Uraiwan Panich, Russel J Reiter, Andrzej T Slominski
Melatonin and its derivatives [N1 -acetyl-N2 -formyl-5-methoxykynurenine (AFMK) and N-acetyl serotonin (NAS)] have broad spectrum protective effects against photocarcinogenesis, including both direct and indirect antioxidative actions, regulation of apoptosis and DNA damage repair; these data were primarily derived from in vitro models. This study evaluates possible beneficial effects of melatonin and its active derivatives against UVB-induced harm to human and porcine skin ex vivo and to cultured HaCaT cells...
April 27, 2018: Journal of Pineal Research
https://www.readbyqxmd.com/read/29694929/3-acetyl-8-methoxy-2-h-chromen-2-one-derived-schiff-bases-as-potent-antiproliferative-agents-insight-into-the-influence-of-4-n-substituents-on-the-in-vitro-biological-activity
#19
G Kalaiarasi, S Rex Jeya Rajkumar, G Aswini, S Dharani, Frank R Fronczek, R Prabhakaran
A series of 3-acetyl-8-methoxycoumarin appended thiosemicarbazones (1-4) was prepared from the reaction of 3-acetyl-8-methoxycoumarin with 4(N)-substituted thiosemicarbazides in a view of ascertaining their biological properties with the change of N-terminal substitution in the thiosemicarbazide moiety. Comprehensive characterization was brought about by various spectral and analytical methods. The molecular structures of all the compounds were determined by single crystal X-ray diffraction analysis. Binding studies with Calf thymus DNA (CT-DNA) and proteins such as Bovine Serum Albumin (BSA) and Human Serum Albumin (HSA) indicated an intercalative mode of binding with DNA and static quenching mechanism with proteins...
April 14, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29693177/the-therapeutic-effect-of-artesunate-on-rosacea-through-the-inhibition-of-the-jak-stat-signaling-pathway
#20
Ting Li, Qingwen Zeng, Xingming Chen, Guojiang Wang, Haiqing Zhang, Aihua Yu, Hairui Wang, Yang Hu
Acne rosacea is a type of chronic dermatosis with the characteristics of erubescence, angiotelectasis and pustule formation. However, current treatment methods are limited due to the side effects. Artesunate demonstrated a promising therapeutic efficacy with a high safety margin. HaCaT cells were treated with antibacterial peptide LL‑37 to simulate rosacea caused by Demodex folliculorum (D. folliculorum) infection. Cell Counting kit 8 and flow cytometry assays were performed to measure cellular proliferation, apoptosis, the stage of the cell cycle and reactive oxygen species generation in order to determine the level of cell damage...
April 16, 2018: Molecular Medicine Reports
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