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https://www.readbyqxmd.com/read/28931048/mir-181a-decelerates-proliferation-in-cutaneous-squamous-cell-carcinoma-by-targeting-the-proto-oncogene-kras
#1
Johannes Neu, Piotr Jan Dziunycz, Andreas Dzung, Karine Lefort, Martin Falke, Rémy Denzler, Sandra Nicole Freiberger, Guergana Iotzova-Weiss, Aleksandar Kuzmanov, Mitchell Paul Levesque, Gian-Paolo Dotto, Günther Franz L Hofbauer
Cutaneous squamous cell carcinoma (SCC) is the second most common human skin cancer with a rapidly increasing incidence among the Caucasian population. Among the many regulators, responsible for cancer progression and growth, microRNAs (miRNA) are generally accepted as key players by now. In our current study we found that microRNA-181a (miR-181a) shows low abundance in SCC compared to normal epidermal skin. In vitro, miRNA downregulation in normal primary keratinocytes induced increased proliferation, while in vivo miR-181a downregulation in HaCaT normal keratinocytes showed tumor-like growth increase up to 50%...
2017: PloS One
https://www.readbyqxmd.com/read/28926995/3-bromo-4-5-dihydroxybenzaldehyde-enhances-the-level-of-reduced-glutathione-via-the-nrf2-mediated-pathway-in-human-keratinocytes
#2
Ki Cheon Kim, Yu Jae Hyun, Susara Ruwan Kumara Madduma Hewage, Mei Jing Piao, Kyoung Ah Kang, Hee Kyoung Kang, Young Sang Koh, Mee Jung Ahn, Jin Won Hyun
A natural bromophenol found in seaweeds, 3-bromo-4,5-dihydroxybenzaldehyde (BDB), has been shown to possess antioxidant effects. This study aimed to investigate the mechanism by which BDB protects skin cells subjected to oxidative stress. The effect of BDB on the protein and mRNA levels of glutathione-related enzymes and the cell survival of human keratinocytes (HaCaT cells) was investigated. BDB treatment increased the protein and mRNA levels of glutathione synthesizing enzymes and enhanced the production of reduced glutathione in HaCaT cells...
September 18, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28924372/il-36%C3%AE-inhibits-differentiation-and-induces-inflammation-of-keratinocyte-via-wnt-signaling-pathway-in-psoriasis
#3
Wenming Wang, Xiaoling Yu, Chao Wu, Hongzhong Jin
Psoriasis is a common inflammatory skin disease characterized by abnormal keratinocyte inflammation and differentiation that has a major impact on patients' quality of life. IL-36γ, a member of IL-36 cytokine family, is highly expressed in psoriasis and plays an important role in inflammation response and differentiation. However, the function of IL-36γ in differentiation and inflammation of keratinocyte in psoriasis has not been clearly identified. Thus, this study aimed to investigate the role of IL-36γ on differentiation and inflammation in HaCaT cells...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28923602/cell-wall-mannoprotein-of-candida-albicans-induces-cell-cycle-alternation-and-inhibits-apoptosis-of-hacat-cells-via-nf-%C3%AE%C2%BAb-signal-pathway
#4
Yang Han, Hang-Hang Jiang, Yu-Jing Zhang, Xing-Jia Hao, Yu-Zhe Sun, Rui-Qun Qi, Hong-Duo Chen, Xing-Hua Gao
Candida albicans (C. albicans) is a commensal organism in human and a well-known dimorphic opportunistic pathogenic fungus. Though plenty of researches on the pathogenesis of C. albicans have been performed, the mechanism is not fully understood. The cell wall components of C. albicans have been documented to play important roles in its pathogenic processes. To further study the infectious mechanism of C. albicans, we investigated the potential functional role of its cell wall mannoprotein in cell cycle and apoptosis of HaCaT cells...
September 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28918452/pro-oxidant-status-and-nrf2-levels-in-psoriasis-vulgaris-skin-tissues-and-dimethyl-fumarate-treated-hacat-cells
#5
Yoon Jin Lee, Jin Ho Bae, Sang-Gue Kang, Sung Woo Cho, Dong-Il Chun, Seung Min Nam, Chul Han Kim, Hae Seon Nam, Seon Hwa Lee, Sang Han Lee, Moon Kyun Cho
Reactive oxygen species (ROS) contribute to pathogenesis of many inflammatory skin diseases, including psoriasis. The aim of this study is to compare antioxidant protein expression in psoriasis vulgaris (PV) skin tissues with that in normal skin tissues in vivo and to evaluate the effects of dimethyl fumarate (DMF), used for the treatment of psoriasis, on ROS generation and apoptosis in a human keratinocyte cell line HaCaT. Compared with normal skin tissues, PV skin tissues showed increased protein oxidation as well as down-regulation of Nrf2 and its regulatory proteins such as HO-1 and AKR1C3...
September 16, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28918125/iodinated-chlorin-p6-copper-complex-induces-anti-proliferative-effect-in-oral-cancer-cells-through-elevation-of-intracellular-reactive-oxygen-species
#6
Paromita Sarbadhikary, Alok Dube
We investigated the anticancer chemotoxicity of previously reported iodinated chlorin p6 copper complex (ICp6-Cu), a novel chlorophyll derivative in which copper is attached to the side chain carboxylate groups via coordination. Human oral carcinoma cells NT8e, 4451 and the non-cancerous keratinocyte HaCaT cells were treated with ICp6-Cu for 48 h in dark and cell viability, proliferation and morphological alterations were examined. ICp6-Cu showed pronounced cytotoxicity in cancer cells with IC50 ∼40 μM, whereas, the viability of HaCaT cells was not affected...
September 13, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28916288/anti-apoptotic-and-moderate-anti-inflammatory-effects-of-berberine-in-sulfur-mustard-exposed-keratinocytes
#7
Simon Lang, Tanja Popp, Christian Silvester Kriegs, Annette Schmidt, Frank Balszuweit, Georg Menacher, Kai Kehe, Horst Thiermann, Thomas Gudermann, Dirk Steinritz
Skin affections after sulfur mustard (SM) exposure include erythema, blister formation and severe inflammation. An antidote or specific therapy does not exist. Anti-inflammatory compounds as well as substances counteracting SM-induced cell death are under investigation. In this study, we investigated the benzylisoquinoline alkaloide berberine (BER), a metabolite in plants like berberis vulgaris, which is used as herbal pharmaceutical in Asian countries, against SM toxicity using a well-established in vitro approach...
September 12, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28916287/effects-of-anti-inflammatory-compounds-on-sulfur-mustard-injured-cells-recommendations-and-caveats-suggested-by-in-vitro-cell-culture-models
#8
Georg Menacher, Dirk Steinritz, Annette Schmidt, Tanja Popp, Franz Worek, Thomas Gudermann, Horst Thiermann, Frank Balszuweit
Sulfur mustard (SM) is a vesicant agent who had its first military use 100 years ago, in Ypres. Since then it has been used in several conflicts like the Iran-Iraq war in the 1980s. The use of SM in Syria 2015 indicated the still existing threat. Despite decades of research no causal antidote against SM intoxication is available, so far. A SM intoxication is accompanied by necrosis, apoptosis and inflammation. To counteract the SM-induced inflammation, glucocorticoids and non-steroidal anti-inflammatory compounds (NSAIDs) are recommended...
September 12, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28904369/antimutagenic-and-antioxidant-activity-of-the-essential-oils-of-citrus-sinensis-and-citrus-latifolia
#9
J D Toscano-Garibay, M Arriaga-Alba, J Sánchez-Navarrete, M Mendoza-García, J J Flores-Estrada, M A Moreno-Eutimio, J J Espinosa-Aguirre, M González-Ávila, N J Ruiz-Pérez
The essential oils of Citrus sinensis and Citrus latifolia showed antimycotic activity against Candida spp. isolated from the oral cavity; they are neither mutagenic on the Ames test nor cytotoxic. Their main components are R-(+)-limonene, β-thujene, α-myrcene and γ-terpinene. The aim of this work was to evaluate their antimutagenic and antioxidant capacities. Antimutagenic properties were evaluated against MNNG and ENNG on S. typhimurium TA100; against 2AA on strain TA98 and in front of 4NQO and NOR on strain TA102...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28901485/a-novel-non%C3%A2-contact-communication-between-human-keratinocytes-and-t-cells-exosomes-derived-from-keratinocytes-support-superantigen%C3%A2-induced-proliferation-of-resting-t-cells
#10
Xiao-Wei Cai, Rong Zhu, Lei Ran, Yi-Qian Li, Ke Huang, Jing Peng, Wei He, Chun-Li Zhou, Ru-Peng Wang
It is widely accepted that keratinocytes act as non‑professional antigen‑presenting cells and support superantigen‑induced proliferation of resting T cells; however, it remains unknown whether keratinocytes function in situ with T cells via a non‑contact mechanism. The current study used a transwell co‑culture system and demonstrated, for the first time to the best of the authors' knowledge, that HaCaT cells (the human keratinocyte cell line) did induce T cell proliferation via indirect contact. The data further indicated that exosomes, small membrane vesicles that transfer antigens to recipient cells, are also involved in the superantigen‑associated immunity of keratinocytes...
September 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28901448/exposure-of-keratinocytes-to-non%C3%A2-thermal-dielectric-barrier-discharge-plasma-increases-the-level-of-8%C3%A2-oxoguanine-via-inhibition-of-its-repair-enzyme
#11
Ki Cheon Kim, Madduma Hewage Susara Ruwan Kumara, Kyoung Ah Kang, Mei Jing Piao, Min Chang Oh, Yea Seong Ryu, Jin Oh Jo, Young Sun Mok, Jennifer H Shin, Yeunsoo Park, Seong Bong Kim, Suk Jae Yoo, Jin Won Hyun
Oxidative stress enhances cellular DNA oxidation and may cause mutations in DNA bases, including 8‑oxoguanine (8‑oxoG). Our recent study reported that exposure of cells to non‑thermal dielectric barrier discharge (DBD) plasma generates reactive oxygen species and damages DNA. The present study investigated the effect of non‑thermal DBD plasma exposure on the formation of 8‑oxoG in HaCaT human keratinocytes. Cells exposed to DBD plasma exhibited increased level of 8‑oxoG. In addition, mRNA and protein expression levels of 8‑oxoguanine glycosylase 1 (OGG1), an 8‑oxoG repair enzyme, were reduced in plasma‑exposed cells...
September 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28900461/effects-of-wannachawee-recipe-with-antipsoriatic-activity-on-suppressing-inflammatory-cytokine-production-in-hacat-human-keratinocytes
#12
Mingkwan Na Takuathung, Ariyaphong Wongnoppavich, Pornsiri Pitchakarn, Ampai Panthong, Parirat Khonsung, Natthakarn Chiranthanut, Noppamas Soonthornchareonnon, Seewaboon Sireeratawong
Psoriasis is a chronic inflammatory and immune-mediated skin disease. The pathogenesis involves T cells activation via the IL-23/Th17 axis. Conventional treatments of psoriasis have adverse events influencing patients' adherence. Wannachawee Recipe (WCR) has been effectively used as Thai folk remedy for psoriasis patients; however, preclinical evidence defining how WCR works is still lacking. This study defined mechanisms for its antiproliferation and anti-inflammatory effects in HaCaT cells. The cytotoxicity and antiproliferation results from SRB and CCK-8 assays showed that WCR inhibited the growth and viability of HaCaT cells in a concentration-dependent manner...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28900100/oscillations-of-ultra-weak-photon-emission-from-cancer-and-non-cancer-cells-stressed-by-culture-medium-change-and-tnf-%C3%AE
#13
Pierre Madl, Thomas Verwanger, Mark Geppert, Felix Scholkmann
Cells spontaneously emit photons in the UV to visible/near-infrared range (ultra-weak photon emission, UPE). Perturbations of the cells' state cause changes in UPE (evoked UPE). The aim of the present study was to analyze the evoked UPE dynamics of cells caused by two types of cell perturbations (stressors): (i) a cell culture medium change, and (ii) application of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α). Four types of human cell lines were used (squamous cell carcinoma cells, A431; adenocarcinomic alveolar basal epithelial cells, A549; p53-deficient keratinocytes, HaCaT, and cervical cancer cells, HeLa)...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28899779/eupatilin-with-ppar%C3%AE-agonistic-effects-inhibits-tnf%C3%AE-induced-mmp-signaling-in-hacat-cells
#14
Yujung Jung, Jin-Chul Kim, Yongsoo Choi, Sullim Lee, Ki Sung Kang, Yong Kee Kim, Su-Nam Kim
Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is a flavonoid compound exhibiting several beneficial biological activities, including neuroprotection, anti-cancer, antinociception, chondroprotection, anti-oxidation, and anti-inflammation. Our previous study demonstrated that eupatilin specifically activates peroxisome proliferator-activated receptor alpha (PPARα) through direct binding. The PPAR subfamily includes ligand-dependent transcription factors that consist of three isotypes: PPARα, PPARβ/δ, and PPARγ...
September 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28894278/agerarin-identified-from-ageratum-houstonianum-stimulates-circadian-clock-mediated-aquaporin-3-gene-expression-in-hacat-keratinocytes
#15
Soon Young Shin, Da Hyun Lee, Ha-Na Gil, Beom Soo Kim, Jeong-Sook Choe, Jung-Bong Kim, Young Han Lee, Yoongho Lim
The juice of Ageratum houstonianum is used in folk medicine as an external wound healing aid for skin injuries. However, the active component of A. houstonianum and its mode of action in skin wound healing has not been investigated. This study was conducted to investigate the effect of A. houstonianum ethanolnolic extract (AHE) on the expression of aquaporin-3 (AQP3), an integral membrane protein for water and glycerol transport in keratinocytes, and to identify the structure of the A. houstonianum bioactive compound...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28886471/dna-damage-by-2-3-7-8-tetrachlorodibenzo-p-dioxin-induced-p53-mediated-apoptosis-through-activation-of-cytochrome-p450-aryl-hydrocarbon-receptor
#16
Durgesh Nandini Das, Prashanta Kumar Panda, Niharika Sinha, Subhadip Mukhopadhyay, Prajna Parimita Naik, Sujit K Bhutia
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD; polycyclic aromatic hydrocarbon) is a persistent and ubiquitous environmental contaminant that causes a wide variety of deleterious effects. In this study, the DNA damage and apoptotic activity induced by TCDD was examined using in silico and in vitro approaches. In silico study showed that conformational changes and energies involved in the binding of TCDD to cytochrome P450 1B1 (CYP1B1) were crucial for its target proteins. Moreover, activated TCDD had high affinity to bind with aryl hydrocarbon receptor (AhR), with a binding energy of -564...
August 18, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28873377/sea-buckthorn-hippopha%C3%A3-rhamnoides-l-oil-improves-atopic-dermatitis-like-skin-lesions-via-inhibition-of-nf-%C3%AE%C2%BAb-and-stat1-activation
#17
Dian-Dong Hou, Zheng-Hong Di, Rui-Qun Qi, He-Xiao Wang, Song Zheng, Yu-Xiao Hong, Hao Guo, Hong-Duo Chen, Xing-Hua Gao
BACKGROUND AND OBJECTIVES: The objective of this study was to evaluate the topical effects of sea buckthorn (SBT) oil on atopic dermatitis (AD)-like lesions in a mouse model generated by repeated topical administration of DNCB in BALB/c mice. METHODS: DNCB was applied repeatedly on the dorsal skin of mice to induce AD-like lesions. Following AD induction, SBT oil was applied daily on the dorsal skin for 4 weeks. The severity of skin lesions was examined macroscopically and histologically...
2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28872682/%C3%AE-2-himachalen-6-ol-protects-against-skin-cancer-development-in%C3%A2-vitro-and-in%C3%A2-vivo
#18
Hamid E Daaboul, Costantine F Daher, Robin I Taleb, Joelle Boulos, Kikki Bodman-Smith, Petra Boukamp, Wassim N Shebaby, Carol Dagher, Mirvat El-Sibai, Mohamad A Mroueh
BACKGROUND: Previous studies in our laboratory showed that Daucus carota oil extract (DCOE) possesses remarkable in-vitro anticancer activity and antitumour promoting effect against DMBA/TPA skin carcinogenesis in mice. Chemical analysis of DCOE led to the isolation of the β-2-himachalen-6-ol (HC), major sesquiterpene with a potent anticancer activity against various colon, breast, brain and skin cancer cells. This study investigated the anticancer activity of HC against invasive epidermal squamous cell carcinoma cells and evaluated its effect in a DMBA/TPA skin carcinogenesis Balb/c murine model...
September 5, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28870900/probing-the-molecular-mechanisms-governing-the-oncolytic-activity-of-paeonia-suffruticosa-on-triple-negative-breast-cancer-cells-in-vitro
#19
Dongyeon Kim, Daniel Radin, Donna Leonardi
BACKGROUND/AIM: Extracts of Paeonia suffruticosa are traditionally used in Chinese medicine to increase blood flow. Recently, this extract has been shown to possess anti-tumor and anti-inflammatory properties, though this mechanism remains unknown. In the current work, we prepared extracts of P. suffruticosa and analyzed their effects on MDA-MB-231 triple-negative breast cancer cells. MATERIALS AND METHODS: Varying concentrations of an aqueous extract of P. suffruticosa was administered to MDA-MB-231...
September 2017: Anticancer Research
https://www.readbyqxmd.com/read/28867735/effects-of-ascorbyl-2-phosphate-magnesium-on-human-keratinocyte-toxicity-and-pathological-changes-by-sorafenib
#20
Kazuhiro Yamamoto, Hiroaki Shichiri, Takahiro Ishida, Kenta Kaku, Tatsuya Nishioka, Manabu Kume, Hiroo Makimoto, Tsutomu Nakagawa, Takeshi Hirano, Toshinori Bito, Chikako Nishigori, Ikuko Yano, Midori Hirai
Hand-foot skin reaction is recognized as one of the most common adverse events related to multiple tyrosine kinase inhibitors, but an effective prevention method has not been identified. The chief aim of this study was to find a mechanism-based preventive method for the skin toxicity induced by sorafenib using vitamin C derivatives. The effects of ascorbyl-2-phosphate magnesium (P-VC-Mg) on the molecular and pathological changes induced by sorafenib were investigated in human keratinocyte HaCaT cells. The cell growth inhibition and apoptotic effects of sorafenib were attenuated by P-VC-Mg...
2017: Biological & Pharmaceutical Bulletin
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