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Xiaojing Li, Zhifeng Li, Xianping Li, Baoguo Liu, Zhijun Liu
BACKGROUND: Malignant melanoma (MM) is one of the high degree of malignancy and early prone to blood and lymph node metastasis. There is not cured for MM. Tan II A has been reported to reduce cancer cell proliferation. But the mechanism by which Tan II A inhibited melanoma growth are not well characterized. We sought to explore the possible mechanism by which Tan II A regulated cell proliferation through autophagy signaling pathway in A375 cells. METHODS: We tested the effects of Tan II A on melanoma A375, MV3, M14, and other human cell lines including Hacat and HUVEC cells in cell culture model...
May 22, 2017: BMC Cancer
Xing Zhang, Kimberly Kew, Richard Reisdorph, Mark Sartain, Roger Powell, Michael L Armstrong, Kevin Quinn, Charmion Cruickshank-Quinn, Scott Walmsley, Samantha S Bokatzian, Ed Darland, Matthew Rain, Kenneth Imatani, Nichole Reisdorph
A commercial liquid chromatography/drift tube ion mobility-mass spectrometer (LC/IM-MS) was evaluated for its utility in global metabolomics analysis. Performance was assessed using twelve targeted metabolite standards where the limit of detection (LOD), linear dynamic range, resolving power, and collision cross section (Ω), is reported for each standard. Data was collected in three different instrument operation modes: flow injection analysis with IM-MS (FIA/IM-MS), LC/MS and LC/IM-MS. Metabolomics analyses of human plasma and HaCaT cells were used to compare the above three operation modes...
May 22, 2017: Analytical Chemistry
Louise Saïdi, Carla Vilela, Helena Oliveira, Armando J D Silvestre, Carmen S R Freire
The present study reports the development of non-cytotoxic and pH-sensitive nanostructured membranes consisting of a polymer with amino acid pending moieties and bacterial nanocellulose (BC). The nanocomposites were prepared through a simple methodology under green reaction conditions. The obtained materials display good thermal stability (up to 200°C), viscoelastic (storage modulus>700MPa) and mechanical (Young's modulus=3.5-4.9GPa) properties, together with high water uptake capacity. The results of the in vitro MTT assay showed that the nanocomposites are non-cytotoxic to HaCaT cells for 72h...
August 1, 2017: Carbohydrate Polymers
Rebecca Justiniano, Jessica Perer, Anh Hua, Mohammad Fazel, Andrea Krajisnik, Christopher M Cabello, Georg T Wondrak
Nonmelanoma skin cancer (NMSC) is the most common malignancy in the United States representing a considerable public health burden. Pharmacological suppression of skin photo-carcinogenesis has shown promise in preclinical and clinical studies, but more efficacious photochemopreventive agents are needed. Here, we tested feasibility of harnessing pharmacological disruption of intracellular zinc-homeostasis for photochemoprevention in vitro and in vivo. Employing the zinc-ionophore and FDA-approved microbicidal agent zinc pyrithione (ZnPT), used worldwide in over-the-counter (OTC) topical consumer products, we first demonstrated feasibility of achieving ZnPT-based intracellular Zn(2+) -overload in cultured malignant keratinocytes (HaCaT-ras II-4; SCC-25) employing membrane-permeable fluorescent probes...
May 15, 2017: Photochemistry and Photobiology
Hui Shen, Bijun Zeng, Chang Wang, Xueyong Tang, Haizhen Wang, Wen Liu, Zhibo Yang
Psoriasis is a common chronic inflammatory skin disease which is characterized by hyperproliferation and aberrant differentiation of keratinocytes; however the exact pathogenesis is largely unknown. Interleukin-22 (IL-22) has demonstrated its vital role in T cell-mediated immune response by interacting with keratinocytes in the pathogenesis of psoriasis. The microRNAs (miRNAs) are a class of small non-coding RNA molecules that play important roles in cellular processes by regulating gene expression at the post-transcriptional level...
May 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Hiroshi Koga, Norito Ishii, Takashi Hashimoto, Takekuni Nakama
Pemphigus herpetiformis (PH) is a rare variant of pemphigus characterized by erythemas and vesicles, tending to present with annular-shaped lesions. Immunologically, immunoglobulin (Ig)G deposition at the keratinocyte cell surfaces is observed. Linear IgA bullous dermatosis (LABD) is a rare subepidermal blistering disease with linear IgA deposits at the epidermal basement membrane zone (BMZ). The annular-shaped skin lesions in PH mimic clinical manifestation of other autoimmune bullous diseases, including LABD, although PH and LABD have different immunological and histopathological features...
February 2017: Journal of Dermatology
Luis A C Tietbohl, Adriana P Oliveira, Ricardo S Esteves, Ricardo D D G Albuquerque, Diogo Folly, Francisco P Machado, Arthur L Corrêa, Marcelo G Santos, Ana L G Ruiz, Leandro Rocha
Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell)...
May 2, 2017: Anais da Academia Brasileira de Ciências
Wasim Akhtar, Garima Verma, Mohemmed Faraz Khan, Mohammad Shaquiquzzaman, Arpana Rana, Tarique Anwer, Mymoona Akhter, M Mumtaz Alam
A series of dihydropyrimidine and pyridazinone hybrids (5a-u) was synthesized by cyclo-condensation of appropriate 3-aroylpropionic acid and dihydropyrimidine-hydrazine derivatives with the objective to develop agents with improved COX-2 inhibition for the treatment of breast cancer. The synthesized compounds were evaluated for their in-vitro anticancer activities against human breast cell lines (MCF-7, MDA-MB-231) and normal human keratinocytes (HaCaT). Amongst the compounds tested, compound 5l was found to be active against both the cell lines with IC50 value of 3...
February 20, 2017: Mini Reviews in Medicinal Chemistry
Sivakumar Singaravelu, Giriprasath Ramanathan, Uma Tiruchirapalli Sivagnanam
The by-product of the slaughter house was utilized for the development of promising regenerative wound dressing material. Currently, dual-layered nanofibrous spongy scaffold was fabricated for tissue engineering applications. Herein, Keratin (K)-Fibrin (F)-Gelatin (G) 3D sponge loaded with Mupirocin (M) was fabricated with the naturally derived materials from bovine origin using freeze drying method. Moreover, poly(3-hydroxybutyric acid) (P) and Gelatin(G) solution loaded with Curcumin (C) were electrospun to get the dual drug loaded dual-layered nanofibrous spongy 3D scaffold (KFG:M-PG:C)...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Sandeep Karna
BACKGROUND: Prostaglandins (PGs) have short existence in vivo because they are rapidly metabolized by NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) to 15-ketoprostaglandins. Inhibition of 15-PGDH causes elevated level of PGE2 in cellular system. It will be valuable for the therapeutic management of diseases requiring elevated PGE2 levels, like wound healing. OBJECTIVE: Ninety-eight plant samples were screened for the discovery of potent 15-PGDH inhibitor...
January 2017: Pharmacognosy Magazine
Sutthirat Srisuwan, Supayang Piyawan Voravuthikunchai
Methicillin-resistant Staphylococcus aureus (MRSA) has an ability to invade nonprofessional phagocytic cells, resulting in persistent infections and most likely host cell death. Series of our studies have claimed pronounced antibacterial efficacy of Rhodomyrtus tomentosa leaf extract. This study was to further investigate potency of the extract in intracellular killing of human HaCaT keratinocytes. Pretreatment of MRSA with the extract resulted in a remarkable reduction in the bacterial adhesion to HaCaT keratinocytes, compared with untreated control (p < 0...
May 5, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Yunki Lee, Kyong-Hoon Choi, Kyung Min Park, Jong-Min Lee, Bong Joo Park, Ki Dong Park
Various types of commercialized wound dressings (e.g., films, foams, gels, and nanofiber meshes) have been clinically used as a physical barrier against bacterial invasion and as wound-healing materials. Although these dressings can protect the wounded tissue from the external environment, they cannot treat the wounds that are already infected with bacteria. Herein, we report in situ H2O2-releasing hydrogels as an active wound dressing with antibacterial properties for treatment of drug-resistant bacterial infection...
May 10, 2017: ACS Applied Materials & Interfaces
Abdullah A Alangari, Keith Morris, Bashir A Lwaleed, Laurie Lau, Ken Jones, Rose Cooper, Rowena Jenkins
INTRODUCTION: As manuka honey (MH) exhibits immunoregulatory and anti-staphylococcal activities, we aimed to investigate if it could be effective in the treatment of atopic dermatitis (AD). METHODS: Adult volunteers with bilateral AD lesions were asked to apply MH on one site overnight for seven consecutive days and leave the contralateral site untreated as possible. Three Item Severity score was used to evaluate the response. Skin swabs were obtained from both sites before and after treatment to investigate the presence of staphylococci and enterotoxin production...
June 2017: Immunity, Inflammation and Disease
Ichiro Yajima, Mayuko Y Kumasaka, Machiko Iida, Reina Oshino, Hiroko Tanihata, Aeorangajeb Al Hossain, Nobutaka Ohgami, Masashi Kato
Chronic exposure to arsenic is associated with various diseases in humans. Skin hyperpigmentation is the most sensitive objective symptom for patients with arsenicosis. However, there is very limited information about the mechanism of arsenic-mediated skin hyperpigmentation in vivo. In this study, hairless homozygous mice (Hr/Hr-mice) that drank water containing 3 and 30 µM arsenic for 2 months developed skin hyperpigmentation with increased levels of arsenic and number of melanocytes in the skin. Since it is possible for humans to be exposed to 3 µM of arsenic in well drinking water, our results suggest that the Hr/Hr-mice could be a novel model sensitively reflecting arsenic-mediated skin hyperpigmentation...
May 3, 2017: Archives of Toxicology
Tsong-Min Chang, Jen-Horng Tsen, Hsuan Yen, Ting-Ya Yang, Huey-Chun Huang
Periostracum cicadae is widely used for the treatment of skin diseases such as eczema, pruritus, and itching. The current study sought to evaluate the effect of P. cicadae extract on ultraviolet B (UVB) irradiation and identify the mechanisms involved. Photodamage-protective activity of P. cicadae extracts against oxidative challenge was screened using HaCaT keratinocytes. P. cicadae extracts did not affect cell viability but decreased reactive oxygen species (ROS) production. The extract attenuates the expression of interleukin-6 (IL-6), matrix metalloproteinase-2 (MMP-2), and MMP-9 in UVB-treated HaCaT cells...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
Rongkun Dou, Zongying Liu, Xue Yuan, Danzhou Xiangfei, Ruixue Bai, Zhenfei Bi, Piao Yang, Yalan Yang, Yinsong Dong, Wei Su, Diqiang Li, Canquan Mao
Psoriasis is a chronic and persistent inflammatory skin disease seriously affecting the quality of human life. In this study, we reported an ancient formula of Chinese folk medicine, the natural plant antimicrobial solution (PAMs) for its anti-inflammatory effects and proposed the primary mechanisms on inhibiting the inflammatory response in TNF-α/IFN-γ-induced HaCaT cells and imiquimod-induced psoriasis-like skin disease mouse model. Two main functional components of hydroxysafflor Yellow A and allantoin in PAMs were quantified by HPLC to be 94...
2017: PloS One
Letícia Spinelli Jacoby, Valnês da Silva Rodrigues Junior, Maria Martha Campos, Luciane Macedo de Menezes
INTRODUCTION: The safety of orthodontic materials is a matter of high interest. In this study, we aimed to assess the in-vitro cytotoxicity of orthodontic band extracts, with and without silver solder, by comparing the viability outcomes of the HaCat keratinocytes, the fibroblastic cell lineages HGF and MRC-5, and the kidney epithelial Vero cells. METHODS: Sterilized orthodontic bands with and without silver solder joints were added to culture media (6 cm(2)/mL) and incubated for 24 hours at 37°C under continuous agitation...
May 2017: American Journal of Orthodontics and Dentofacial Orthopedics
Jinghong Yu, Zhicai Xiao, Ruizhi Zhao, Chuanjian Lu, Yuemei Zhang
AIMS: The total glucosides of paeony (TGP) are used to treat psoriasis in the clinic. However, its active components and mechanisms are not clear. Paeoniflorin is the main constituent of TGP. Thus, the anti-psoriasis effect of paeoniflorin was studied, and its mechanism was explored. MATERIAL AND METHODS: The effect of paeoniflorin was evaluated using a psoriasis-like model of guinea pigs. The levels of IL-6, IL-17A, IL-22, p38 MAPK, and ERK1/2 in HaCaT cells stimulated by lipopolysaccharide (LPS) were determined using RT-qPCR, enzyme linked immunosorbent assays (ELISAs) and western blot...
April 26, 2017: Life Sciences
Kyung-Ah Cho, Minhwa Park, Yu-Hee Kim, So-Youn Woo
Although mast cells are traditionally thought to function as effector cells in allergic responses, they have increasingly been recognized as important regulators of various immune responses. Mast cells mature locally; thus, tissue-specific influences are important for promoting mast cell accumulation and survival in the skin and the gastrointestinal tract. In this study, we determined the effects of keratinocytes on mast cell accumulation during Th17-mediated skin inflammation. We observed increases in dermal mast cells in imiquimod-induced psoriatic dermatitis in mice accompanied by the expression of epidermal stem cell factor (SCF), a critical mast cell growth factor...
June 10, 2017: Biochemical and Biophysical Research Communications
Emale El Darzi, Samer Bazzi, Sarah Daoud, Karim S Echtay, Georges M Bahr
Keratinocytes are routinely subjected to both internal and external stimulation. This study investigates the effects of interferon gamma, interleukin-4, tumor necrosis factor alpha, and the synthetic immunomodulator muramyl dipeptide on the human keratinocyte cell line, HaCaT. Following HaCaT stimulation with cytokines or muramyl dipeptide for different time periods, changes in the expression of different cell surface receptors, cell proliferation, and cell apoptosis were evaluated by flow cytometry, tritiated thymidine uptake, and annexin-V staining, respectively...
April 1, 2017: International Journal of Immunopathology and Pharmacology
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