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https://www.readbyqxmd.com/read/27923644/s-and-n-alkylating-agents-diminish-the-fluorescence-of-fluorescent-dye-stained-dna
#1
Robert Giesche, Harald John, Kai Kehe, Annette Schmidt, Tanja Popp, Frank Balzuweit, Horst Thiermann, Thomas Gudermann, Dirk Steinritz
Sulfur mustard (SM), a chemical warfare agent, causes DNA alkylation, which is believed to be the main cause of its toxicity. SM DNA adducts are commonly used to verify exposure to this vesicant. However, the required analytical state-of-the-art mass-spectrometry methods are complex, use delicate instruments, are not mobile, and require laboratory infrastructure that is most likely not available in conflict zones. Attempts have thus been made to develop rapid detection methods that can be used in the field...
December 3, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27923599/the-role-of-the-atm-chk-p53-pathway-in-mediating-dna-damage-in-hand-foot-syndrome-induced-by-pld
#2
Jie Yang, Long Qiao, Zhen Zeng, Junnai Wang, Tao Zhu, Juncheng Wei, Mingfu Wu, Shuangmei Ye, Xiaoyuan Huang, Ding Ma, Ronghua Liu, Qinglei Gao
Pegylated liposomal doxorubicin (PLD) has been approved to treat patients with various types of cancers because it rarely caused side effects, such as cardiotoxicity, in comparison to doxorubicin, but it frequently results in hand-foot syndrome (HFS). This may affect the quality of life and require a reduction in the PLD dose. The pathophysiology of HFS was not well understood. This study was aimed at exploring the mechanism of HFS induced by PLD. We compared the effects of different doses of PLD on the proliferation inhibition and apoptosis in vitro in HaCaT cells and analyzed the skin changes and skin cell DNA damage in vivo using a zebrafish model...
December 3, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/27919950/development-of-a-novel-experimental-in-vitro-model-of-isothiocyanate-induced-apoptosis-in-human-malignant-melanoma-cells
#3
Theodora Mantso, Aristeidis P Sfakianos, Aithne Atkinson, Ioannis Anestopoulos, Melina Mitsiogianni, Sotirios Botaitis, Sebachedin Perente, Constantinos Simopoulos, Stavros Vasileiadis, Rodrigo Franco, Aglaia Pappa, Mihalis I Panayiotidis
BACKGROUND: Isothiocyanates are constituents of cruciferous vegetables which have been associated with reduced cancer risk partially through their ability to induce apoptosis in malignant cells including melanoma. MATERIALS AND METHODS: We have utilized human malignant melanoma (A375), epidermoid carcinoma (A431) and immortalized keratinocyte (HaCaT) cells exposed to various isothiocyanates, under different experimental conditions. RESULTS: An experimental in vitro model utilizing low isothiocyanate concentrations (0...
December 2016: Anticancer Research
https://www.readbyqxmd.com/read/27917896/astragaloside-iv-ameliorates-allergic-inflammation-by-inhibiting-key-initiating-factors-in-the-initial-stage-of-sensitization
#4
Kai-Fan Bao, Xi Yu, Xiao Wei, Li-Li Gui, Hai-Liang Liu, Xiao-Yu Wang, Yu Tao, Guo-Rong Jiang, Min Hong
To illuminate the anti-allergy mechanism of astragaloside IV (AS-IV), we assessed its effects in a murine model of allergic contact dermatitis (ACD). AS-IV administered in the sensitization phase, rather than in the elicitation phase, dramatically alleviated the symptoms of allergic inflammation. We hypothesized that AS-IV exerts its anti-allergy effects by regulating the production of key pro-allergic cytokines based on the fact that interleukin (IL)-33 and thymic stromal lymphopoietin (TSLP) levels increase significantly in the initial stage of the sensitization phase...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27915018/design-and-surface-immobilization-of-short-anti-biofilm-peptides
#5
Biswajit Mishra, Tamara Lushnikova, Radha M Golla, Xiuqing Wang, Guangshun Wang
: Short antimicrobial peptides are essential to keep us healthy and their lasting potency can inspire the design of new types of antibiotics. This study reports the design of a family of eight-residue tryptophan-rich peptides (TetraF2W) obtained by converting the four phenylalanines in temporin-SHf to tryptophans. The temporin-SHf template was identified from the antimicrobial peptide database (http://aps.unmc.edu/AP). Remarkably, the double arginine variant (TetraF2W-RR) was more effective in killing methicillin-resistant Staphylococcus aureus (MRSA) USA300, but less cytotoxic to human skin HaCat and kidney HEK293 cells, than the lysine-containing dibasic combinations (KR, RK and KK)...
November 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27910159/staphylococcus-aureus-derived-membrane-vesicles-exacerbate-skin-inflammation-in-atopic-dermatitis
#6
S H Jun, J H Lee, S I Kim, C W Choi, T I Park, H R Jung, J W Cho, S H Kim, J C Lee
BACKGROUND: Skin colonization or infection with Staphylococcus aureus is known to trigger aggravation of atopic dermatitis (AD). However, the exact mechanisms by which S. aureus can worsen AD are unknown. OBJECTIVE: We investigated whether and how S. aureus-derived membrane vesicles (MVs) contribute to worsening of AD. METHODS: Immunohistochemical and immunoelectron microscopic analyses were performed to detect staphylococcal protein A (SPA) in the epidermis of AD lesions...
November 5, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27901554/a-possible-wound-dressing-material-from-marine-food-waste
#7
Senthil Rethinam, Prabakaran Nivedita, Thiagarajan Hemalatha, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry
PURPOSE: Bluefin Trevally (Caranx melampygus) fish is mainly used for fillet production, the bones of which are discarded as a major solid waste in the fish food processing industry. In the present study, novel collagen films were prepared using the bones of Bluefin Trevally (BT). The study investigates the potential of using this collagen film as a wound dressing material. METHODS: The prepared collagen films (CFs) were characterized for their physicochemical properties using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), atomic force microscopy (AFM), tensile strength, elongation at break, etc...
November 29, 2016: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/27895497/microrna-9-suppresses-the-growth-migration-and-invasion-of-malignant-melanoma-cells-via-targeting-nrp1
#8
Dan Xu, Xiaofeng Chen, Quanyong He, Chengqun Luo
MicroRNAs (miRs) are a class of small noncoding RNAs that negatively regulate the gene expression by directly binding to the 3' untranslated region of their target mRNA, thus resulting in mRNA degradation or translational repression. miR-9 has recently been demonstrated to play a role in the development and progression of malignant melanoma (MM), but the regulatory mechanism of miR-9 in the malignant phenotypes of MM still remains largely unknown. In this study, a total of 73 pairs of MM tissues and adjacent normal tissues were collected...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27893713/phosphorylation-of-nfat3-by-cdk3-induces-cell-transformation-and-promotes-tumor-growth-in-skin-cancer
#9
T Xiao, J J Zhu, S Huang, C Peng, S He, J Du, R Hong, X Chen, A M Bode, W Jiang, Z Dong, D Zheng
The nuclear factor of activated T cells (NFAT) family proteins are transcription factors that regulate the expression of pro-inflammatory cytokines and other genes during the immune response. Although the NFAT proteins have been extensively investigated in the immune system, their role in cancer progression remains controversial. Here, we report that NFAT3 is highly expressed in various skin cancer cell lines and tumor tissues. Knockdown of endogenous NFAT3 expression by short hairpin RNA (shRNA) significantly inhibited tumor cell proliferation, colony formation and anchorage-independent cell growth...
November 28, 2016: Oncogene
https://www.readbyqxmd.com/read/27891094/anti-acne-activity-of-italian-medicinal-plants-used-for-skin-infection
#10
Kate Nelson, James T Lyles, Tracy Li, Alessandro Saitta, Eugenia Addie-Noye, Paula Tyler, Cassandra L Quave
Propionibacterium acnes is implicated in the pathogenesis of acne vulgaris, which impacts >85% of teenagers. Novel therapies are in high demand and an ethnopharmacological approach to discovering new plant sources of anti-acne therapeutics could contribute to filling this void in effective therapies. The aims of our study were two-fold: (1) To determine if species identified in ethnopharmacological field studies as having traditional uses for skin and soft tissue infection (SSTI) exhibit significantly more activity against P...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27882780/solid-lipid-nanoparticles-and-nanostructured-lipid-carrier-based-nanotherapeutics-in-treatment-of-psoriasis-a-comparative-study
#11
Rhythm Arora, Sameer S Katiyar, Varun Kushwah, Sanyog Jain
BACKGROUND: The present work focuses on the development of ultra-small solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) encapsulating cyclosporine and calcipotriol, further incorporated into gel, increasing their penetration through the skin. RESEARCH DESIGN AND METHODS: Developed SLN and NLC were characterized regarding particle size, zeta potential, %entrapment efficiency and dispersed into carbopol 934P-NF gel. Gel was further characterized for rheological behavior and spreadability...
November 24, 2016: Expert Opinion on Drug Delivery
https://www.readbyqxmd.com/read/27880883/anti-proliferative-and-anti-inflammatory-effects-of-3%C3%AE-6%C3%AE-16%C3%AE-trihydroxylup-20-29-ene-on-cutaneous-inflammation
#12
Cintia Delai da Silva Horinouchi, Daniel Augusto Gasparin Bueno Mendes, Stefanie Nolte, Priscilla Salles de Brito, Bruna da Silva Soley, Giovani Marino Favero, Valdir Alves Facundo, Adair Roberto Soares Santos, Daniela de Almeida Cabrini, Michel Fleith Otuki
ETHNOPHARMACOLOGICAL RELEVANCE: 3β,6β,16β-Trihydroxylup-20(29)-ene (TTHL) is a triterpene isolated from the flowers of Combretum leprosum, a plant used in folk medicine in the north of Brazil for the treatment of skin disorders. AIM OF THE STUDY: In the present study, TTHL was evaluated as a potential topical anti-inflammatory and anti-proliferative agent through in vivo and in vitro models. MATERIAL AND METHODS: Anti-inflammmatory and anti-proliferative effects of TTHL were assessed using Swiss mice in acute and chronic models of skin inflammation induced by 12-O-tetradecanoylphorbol-acetate (TPA) application...
November 20, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27879026/ionizing-radiation-promotes-ccl27-secretion-from-keratinocytes-through-the-cross-talk-between-tnf-%C3%AE-and-ros
#13
Qian Zhang, Linlin Zhu, Gang Wang, Ye Zhao, Na Xiong, Hegang Bao, Wensen Jin
The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α)...
November 23, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27877060/anti-inflammatory-and-immunomodulatory-effects-of-aquaphilus-dolomiae-extract-on-in-vitro-models
#14
Marie-Françoise Aries, Hélène Hernandez-Pigeon, Clémence Vaissière, Hélène Delga, Antony Caruana, Marguerite Lévêque, Muriel Bourrain, Katia Ravard Helffer, Bertrand Chol, Thien Nguyen, Sandrine Bessou-Touya, Nathalie Castex-Rizzi
BACKGROUND: Atopic dermatitis (AD) is a common skin disease characterized by recurrent pruritic inflammatory skin lesions resulting from structural and immune defects of the skin barrier. Previous studies have shown the clinical efficacy of Avène thermal spring water in AD, and a new microorganism, Aquaphilus dolomiae was suspected to contribute to these unique properties. The present study evaluated the anti-inflammatory, antipruritic, and immunomodulatory properties of ES0, an original biological extract of A...
2016: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/27866615/anti-neoplastic-selenium-nanoparticles-from-idiomarina-sp-pr58-8
#15
Pallavee Srivastava, Meenal Kowshik
Selenium nanoparticles (SeNPs) with novel biological activities, cancer cell selectivity, and low toxicity towards normal cells have gained attention for chemo-therapeutic and chemo-preventive applications. These nanoparticles may be synthesized using micro-organisms, which is the green alternative of nanofabrication. Here we report the intracellular synthesis of SeNPs by the moderate halophilic bacterium, Idiomarina sp. PR58-8 using sodium selenite as the precursor. Characterization of SeNPs by XRD exhibited the characteristic Bragg's peak of hexagonal selenium with a crystallite domain size of 34nm...
December 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27866016/successful-co-encapsulation-of-benzoyl-peroxide-and-chloramphenicol-in-liposomes-by-a-novel-manufacturing-method-dual-asymmetric-centrifugation
#16
Sveinung G Ingebrigtsen, Nataša Škalko-Basnet, Cristiane de Albuquerque Cavalcanti Jacobsen, Ann Mari Holsæter
Encapsulation of more than one active pharmaceutical ingredient into nanocarriers such as liposomes is an attractive approach to achieve a synergic drug effect and less complicated dosing schedules in multi-drug treatment regimes. Liposomal drug delivery in acne treatment may improve drug efficiency by targeted delivery to pilosebaceous units, reduce adverse effects and improve patient compliance. We therefore aimed to co-encapsulate benzoyl peroxide (BPO) and chloramphenicol (CAM) into liposomes using the novel liposome processing method - dual asymmetric centrifugation (DAC)...
November 16, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27864007/six-generations-of-epidermolytic-palmoplantar-keratoderma-associated-with-a-krt9-r163w-mutation
#17
Peng Wang, Xiao-Jing Kang, Xiao-Hui Tang, Jian-Yong Liu, Wen-Zheng Li, Wei-Jia Wang, Sheng-Nan Liang, Yan-Yan Feng, Yuan Ding, Wen-Jing Chen
Epidermolytic palmoplantar keratoderma (EPPK) is a rare autosomal dominant skin disorder characterized by diffuse hyperkeratosis on the palms and soles. Whole-exome sequencing (WES) has become a powerful tool for the detection of rare causal variants of Mendelian disorders. However, no causal gene for EPPK in the Uygur population has been identified until now, and no treatment exists than can address the underlying pathology.WES analysis was undertaken on two individuals from a large Uygur EPPK pedigree whose disease locus mapped to 17q21...
November 2016: Cancer Genetics
https://www.readbyqxmd.com/read/27860076/incoming-human-papillomavirus-16-genome-is-lost-in-pml-protein-deficient-hacat-keratinocytes
#18
Malgorzata Bienkowska-Haba, Wioleta Luszczek, Timothy R Keiffer, Lucile G M Guion, Stephen DiGiuseppe, Rona S Scott, Martin Sapp
Human papillomaviruses (HPVs) target PML nuclear bodies (NBs) during infectious entry and PML protein is important for efficient transcription of incoming viral genome. However, the transcriptional down regulation was shown to be promoter-independent in that heterologous promoters delivered by papillomavirus particles were also affected. To further investigate the role of PML protein in HPV entry, we used shRNA to knockdown PML protein in HaCaT keratinocytes. Confirming previous findings, PML knockdown in HaCaT cells reduced HPV16 transcript levels significantly following infectious entry without impairing binding and trafficking...
November 17, 2016: Cellular Microbiology
https://www.readbyqxmd.com/read/27856305/cyr61-ccn1-is-involved-in-the-pathogenesis-of-psoriasis-vulgaris-via-promoting-il-8-production-by-keratinocytes-in-a-jnk-nf-%C3%AE%C2%BAb-pathway
#19
Pinru Wu, Gang Ma, Xianjin Zhu, Ting Gu, Jie Zhang, Yue Sun, Hui Xu, Rongfen Huo, Beiqing Wang, Baihua Shen, Xiangdong Chen, Ningli Li
PURPOSE: Interleukin-8 (IL-8) is an important factor in the pathogenesis of psoriasis vulgaris, which is characterized by proliferation of keratinocytes, neutrophil infiltration and angiogenesis. Cysteine-rich 61 (Cyr61/CCN1), a secreted extracellular matrix protein, is a novel proinflammatory factor. Whether Cyr61 is involved in the development of psoriasis vulgaris via IL-8 production remains unknown. In this study we explore the role of Cyr61 in IL-8 expression regulation in vivo and in vitro...
November 14, 2016: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/27855495/pharmacological-importance-characterization-and-applications-of-gold-and-silver-nanoparticles-synthesized-by-panax-ginseng-fresh-leaves
#20
Priyanka Singh, Hina Singh, Sungeun Ahn, Verónica Castro-Aceituno, Zuly Jiménez, Shakina Yesmin Simu, Yeon Ju Kim, Deok Chun Yang
Previously, we showed the rapid and eco-friendly synthesis of gold and silver nanoparticles within 3 and 45 min by fresh leaves extract of herbal medicinal plant Panax ginseng. In addition, we characterized the nanoparticles in terms of shape, size, morphology and stability by FE-TEM, EDX, elemental mapping, SEAD, XRD and particles size analysis. In addition of this, we showed their antimicrobial, anti-coagulant, and biofilm inhibition activity of nanoparticles. Continuing our previous study, here we highlight the further characterization and biomedical applications of P...
November 18, 2016: Artificial Cells, Nanomedicine, and Biotechnology
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