Dan Zhao, Zeng-Kang Huang, Yu Liang, Zhi-Jun Li, Xue-Wei Zhang, Kun-Hang Li, Hao Wu, Xu-Dong Zhang, Chen-Sheng Li, Dong An, Xue Sun, Ming-Xin An, Jun-Xiu Shi, Yi-Jun Bao, Li Tian, Di-Fei Wang, An-Hua Wu, Yu-Hua Chen, Wei-Dong Zhao
BACKGROUND: Microvascular complications are the major outcome of type 2 diabetes progression, and the underlying mechanism remains to be determined. METHODS: High-throughput RNA sequencing was performed using human monocyte samples from controls and diabetes. The transgenic mice expressing human CTSD (cathepsin D) in the monocytes was constructed using CD68 promoter. In vivo 2-photon imaging, behavioral tests, immunofluorescence, transmission electron microscopy, Western blot analysis, vascular leakage assay, and single-cell RNA sequencing were performed to clarify the phenotype and elucidate the molecular mechanism...
February 29, 2024: Circulation Research