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https://www.readbyqxmd.com/read/28637908/rapid-filter-based-detection-and-culture-of-burkholderia-pseudomallei-from-small-volumes-of-urine
#1
Pierre A Michel, Christine Lascols, Jay E Gee, Linda M Weigel, David Sue
Clinical outcomes of melioidosis patients improve when the infecting agent, Burkholderia pseudomallei, is rapidly detected and identified by laboratory testing. Detection of B. pseudomallei DNA or recovery of the pathogen by culture from urine can support a diagnosis of melioidosis and guide patient care. Two new methods designated as Filter-Capture DNA Isolation (FCDI) and Filter Cellular Recovery (FCR) were developed to increase the sensitivity of detection and recovery of viable B. pseudomallei cells from small volumes (0...
June 21, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28636190/uchl1-promotes-invasion-of-breast-cancer-cells-through-activating-akt-signaling-pathway
#2
Yanhong Luo, Jianfeng He, Chunlin Yang, Matthew Orange, Xingcong Ren, Nick Blair, Tao Tan, Jin-Ming Yang, Hua Zhu
As a de-ubiquitin enzyme, ubiquitin C-terminal hydrolase (UCH)-L1 has been shown to be overexpressed in several human cancers. However, the function of UCH-L1 in invasion of breast cancers is still unclear. Here we report that the expression of UCH-L1 is significantly higher in cancer cells with higher invasive ability. While ectopic UCH-L1 expression failed to alter cell proliferation in MCF-7 cells, it caused a significant upregulation of cellular invasion. Furthermore, siRNA mediated knockdown of UCH-L1 led to suppression of invasion in UCH-L1 overexpressing MCF-7 cells...
June 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28631300/a-bacterial-chloroform-reductive-dehalogenase-purification-and-biochemical-characterization
#3
Bat-Erdene Jugder, Susanne Bohl, Helene Lebhar, Robert D Healey, Mike Manefield, Christopher P Marquis, Matthew Lee
We report herein the purification of a chloroform (CF)-reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23-fold increase in relative purity compared to crude lysate. The membrane fraction obtained by ultracentrifugation was solubilized in Triton X-100 in the presence of glycerol, followed by purification by anion exchange chromatography. The molecular mass of the purified TmrA was determined to be 44.5 kDa by SDS-PAGE and MALDI-TOF/TOF...
June 20, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28628613/immunosuppressive-and-angiogenic-cytokine-profile-associated-with-bartonella-bacilliformis-infection-in-post-outbreak-and-endemic-areas-of-carrion-s-disease-in-peru
#4
Maria J Pons, Cláudia Gomes, Ruth Aguilar, Diana Barrios, Miguel Angel Aguilar-Luis, Joaquim Ruiz, Carlota Dobaño, Juana Del Valle-Mendoza, Gemma Moncunill
Analysis of immune responses in Bartonella bacilliformis carriers are needed to understand acquisition of immunity to Carrion's disease and may allow identifying biomarkers associated with bacterial infection and disease phases. Serum samples from 144 healthy subjects from 5 villages in the North of Peru collected in 2014 were analyzed. Four villages had a Carrion's disease outbreak in 2013, and the other is a traditionally endemic area. Thirty cytokines, chemokines and growth factors were determined in sera by fluorescent bead-based quantitative suspension array technology, and analyzed in relation to available data on bacteremia quantified by RT-PCR, and IgM and IgG levels measured by ELISA against B...
June 19, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28627513/a-bioinspired-and-biocompatible-ortho-sulfiliminyl-phenol-synthesis
#5
Feng Xiong, Liang Lu, Tian-Yu Sun, Qian Wu, Dingyuan Yan, Ying Chen, Xinhao Zhang, Wei Wei, Yi Lu, Wei-Yin Sun, Jie Jack Li, Jing Zhao
Synthetic methods inspired by Nature often offer unique advantages including mild conditions and biocompatibility with aqueous media. Inspired by an ergothioneine biosynthesis protein EgtB, a mononuclear non-haem iron enzyme capable of catalysing the C-S bond formation and sulfoxidation, herein, we discovered a mild and metal-free C-H sulfenylation/intramolecular rearrangement cascade reaction employing an internally oxidizing O-N bond as a directing group. Our strategy accommodates a variety of oxyamines with good site selectivity and intrinsic oxidative properties...
June 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28619613/does-supplemental-photodynamic-therapy-optimize-the-disinfection-of-bacteria-and-endotoxins-in-one-visit-and-two-visit-root-canal-therapy-a-randomized-clinical-trial
#6
Diego G D Rabello, Bruna J M Corazza, Luciana L Ferreira, Mauro P Santamaria, Ana P M Gomes, Frederico C Martinho
AIM: to evaluate the effectiveness of supplemental photodynamic therapy (PDT) in optimizing the removal of bacteria and endotoxins from primarily infected root canals after one-visit and two-visit treatments. METHODOLOGY: Twenty-four primarily infected root canals with apical periodontitis were selected and randomly divided into one-visit (n=12) and two-visit treatment groups (n=12). Chemo-mechanical preparation (CMP) was performed by using the single-file reciprocating technique+2...
June 12, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28602400/novel-protein-interactions-with-an-actin-homolog-mreb-of-helicobacter-pylori-determined-by-bacterial-two-hybrid-system
#7
Reyna Cristina Zepeda Gurrola, Yajuan Fu, Isabel Cristina Rodríguez Luna, Claudia Guadalupe Benítez Cardoza, María de Jesús López López, Yolanda López Vidal, Germán Rubén Aguilar Gutíerrez, Mario A Rodríguez Pérez, Xianwu Guo
The bacterium Helicobacter pylori infects more than 50% of the world population and causes several gastroduodenal diseases, including gastric cancer. Nevertheless, we still need to explore some protein interactions that may be involved in pathogenesis. MreB, an actin homolog, showed some special characteristics in previous studies, indicating that it could have different functions. Protein functions could be realized via protein-protein interactions. In the present study, the MreB protein from H. pylori 26695 fused with two tags 10×His and GST in tandem was overexpressed and purified from Escherchia coli...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28592242/an-avirulent-burkholderia-pseudomallei-%C3%A2-purm-strain-with-atypical-type-b-lps-expansion-of-the-toolkit-for-biosafe-studies-of-melioidosis
#8
Michael H Norris, Md Siddiqur Rahman Khan, Herbert P Schweizer, Apichai Tuanyok
BACKGROUND: The work was undertaken to expand the tools available for researching Burkholderia pseudomallei (Bp), the etiological agent of the tropical disease melioidosis. Melioidosis has the potential to pose a severe threat to public health and safety. In the United States, Bp is listed as a Tier-1 select agent by the Centers for Disease Control and Prevention (CDC), thus requiring high levels of regulation and biosafety level 3 (BSL3) facilities for experimental manipulation of live organisms...
June 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28589472/clinical-comparison-of-the-effectiveness-of-7-and-14-day-intracanal-medications-in-root-canal-disinfection-and-inflammatory-cytokines
#9
Frederico C Martinho, Cinthya C Gomes, Gustavo N Nascimento, Ana P M Gomes, Fábio R M Leite
OBJECTIVE: This clinical study compared the effectiveness of 7- and 14-day intracanal medications in the reduction of bacteria/endotoxins from primarily infected root canals and determined their antigenicity against macrophages through the levels of cytokines. METHODS: Seventy-two primarily infected teeth were randomly divided into six groups according to medication and time of application: 7-day groups = G1, Ca(OH)2 + saline solution (SSL); G2, Ca(OH)2 + 2% chlorhexidine (CHX) gel; and G3, 2% CHX gel and 14-day groups = G4, Ca(OH)2 + SSL; G5, Ca(OH)2 + 2% CHX gel; and G6, 2% CHX gel (all groups, n = 12)...
June 6, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28559404/peripheral-t-cell-reactivity-to-heat-shock-protein-70-and-its-cofactor-grpe-from-tropheryma-whipplei-is-reduced-in-patients-with-classical-whipple-s-disease
#10
Lucia Trotta, Kathleen Weigt, Katina Schinnerling, Anika Geelhaar-Karsch, Gerrit Oelkers, Federico Biagi, Gino Roberto Corazza, Kristina Allers, Thomas Schneider, Ulrike Erben, Verena Moos
Background: Classical Whipple's disease (CWD) is characterized by the lack of specific Th1 response towards Tropheryma (T.) whipplei in genetically predisposed individuals. The cofactor GrpE of heat-shock protein 70 (Hsp70) from T. whipplei was previously identified as B-cell antigen. We tested the capacity of Hsp70 and GrpE to elicit specific pro-inflammatory T-cell responses.Methods: Peripheral mononuclear cells from CWD patients and healthy donors were stimulated with T. whipplei lysate or recombinant GrpE or Hsp70 before CD40L, CD69, perforin, granzyme B, CD107a, and interferon (IFN)γ were determined in T cells by flow cytometry...
May 30, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28556187/lipopolysaccharide-a-possible-molecular-mediator-between-periodontitis-and-coronary-artery-disease
#11
John M Liljestrand, Susanna Paju, Kåre Buhlin, Rutger G Persson, Seppo Sarna, Markku S Nieminen, Juha Sinisalo, Päivi Mäntylä, Pirkko J Pussinen
AIM: We aimed to study how lipopolysaccharide in saliva and serum associates with each other, periodontal microbial burden, periodontitis and coronary artery disease. MATERIALS AND METHODS: The used Parogene-cohort comprised N=505 Finnish adults. Coronary diagnosis was acquired by coronary angiography and the main outcomes were: no significant coronary artery disease (n = 123), stable coronary artery disease (n = 184) and acute coronary syndrome (n = 169). Periodontitis was defined according to clinical and radiographic examinations...
May 27, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28542462/a-human-monocytic-nf-%C3%AE%C2%BAb-fluorescent-reporter-cell-line-for-detection-of-microbial-contaminants-in-biological-samples
#12
Claire Battin, Annika Hennig, Patrick Mayrhofer, Renate Kunert, Gerhard J Zlabinger, Peter Steinberger, Wolfgang Paster
Sensing of pathogens by innate immune cells is essential for the initiation of appropriate immune responses. Toll-like receptors (TLRs), which are highly sensitive for various structurally and evolutionary conserved molecules derived from microbes have a prominent role in this process. TLR engagement results in the activation of the transcription factor NF-κB, which induces the expression of cytokines and other inflammatory mediators. The exquisite sensitivity of TLR signalling can be exploited for the detection of bacteria and microbial contaminants in tissue cultures and in protein preparations...
2017: PloS One
https://www.readbyqxmd.com/read/28532454/proteomic-profiling-of-peripheral-blood-neutrophils-identifies-two-inflammatory-phenotypes-in-stable-copd-patients
#13
Adèle Lo Tam Loi, Susan Hoonhorst, Corneli van Aalst, Jeroen Langereis, Vera Kamp, Simone Sluis-Eising, Nick Ten Hacken, Jan-Willem Lammers, Leo Koenderman
BACKGROUND: COPD is a heterogeneous chronic inflammatory disease of the airways and it is well accepted that the GOLD classification does not fully represent the complex clinical manifestations of COPD and this classification therefore is not well suited for phenotyping of individual patients with COPD. Besides the chronic inflammation in the lung compartment, there is also a systemic inflammation present in COPD patients. This systemic inflammation is associated with elevated levels of cytokines in the peripheral blood, but the precise composition is unknown...
May 22, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28530395/detection-of-prosthetic-joint-infection-based-on-magnetically-assisted-surface-enhanced-raman-spectroscopy
#14
Ariana Fargašová, Anna Balzerová, Robert Prucek, Miroslava Htoutou Sedláková, Kateřina Bogdanová, Jiří Gallo, Milan Kolář, Václav Ranc, Radek Zbořil
Accurate and rapid diagnosis of prosthetic joint infection (PJI) is vital for rational and effective therapeutic management of this condition. Several diagnostic strategies have been developed for discriminating between infected and noninfected cases. However, none of them can reliably diagnose the whole spectrum of clinical presentations of PJI. Here, we report a new method for PJI detection based on magnetically assisted surface enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified magnetic nanoparticles (MNP@Strep) whose surface is functionalized with suitable biotinylated antibodies and then coated with silver nanoparticles by self-assembly...
June 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28527491/development-of-live-attenuated-bacterial-vaccines-targeting-escherichia-coli-heat-labile-and-heat-stable-enterotoxins
#15
Chengru Zhu, Prashanth Setty, Edgar C Boedeker
Enterotoxigenic Escherichia coli (ETEC), defined by the production of heat labile (LT) and/or heat stable (ST) toxins, are major causes of diarrhea in animals, children in developing countries and to travelers. No broadly protective ETEC vaccine is available, largely because of the difficulty in inducing immunity to the small ST molecule. To take advantage of the demonstration (Liu et al., 2011; Zhang et al., 2013, 2010) that genetically produced fusions of mutant ST with LT subunits can induce effective immunity against both toxins, we engineered a live attenuated vaccine vector strain of E...
April 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28526791/pseudomonas-chlororaphis-produces-two-distinct-r-tailocins-that-contribute-to-bacterial-competition-in-biofilms-and-on-roots
#16
Robert J Dorosky, Jun Myoung Yu, Leland S Pierson, Elizabeth A Pierson
R-type tailocins are high molecular weight bacteriocins that resemble bacteriophage tails and are encoded within the genomes of many Pseudomonas species. In this study, analysis of the P. chlororaphis 30-84 R-tailocin gene cluster revealed that it encodes the structural components to produce two R-tailocins of different ancestral origin. Two distinct R-tailocin populations differing in length were observed in UV induced lysates of P. chlororaphis 30-84 via transmission electron microscopy. Mutants defective in the production of one or both R-tailocins demonstrated that the killing spectrum of each tailocin is limited to Pseudomonas species...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28522991/brachyspira-hyodysenteriae-and-b-pilosicoli-proteins-recognized-by-sera-of-challenged-pigs
#17
Vanessa Casas, Arantza Rodríguez-Asiain, Roberto Pinto-Llorente, Santiago Vadillo, Montserrat Carrascal, Joaquin Abian
The spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. Immunoproteomics approaches hold the potential for the identification of new, suitable candidates for subunit vaccines against SD and PIS. These strategies take into account the gene products actually expressed and present in the cells, and thus susceptible of being targets of immune recognition...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28494359/impacts-of-solids-retention-time-on-trace-organic-compound-attenuation-and-bacterial-resistance-to-trimethoprim-and-sulfamethoxazole
#18
Majid Neyestani, Eric Dickenson, Jean McLain, Eduardo Robleto, Channah Rock, Daniel Gerrity
Bacteria can grow in the presence of trimethoprim and sulfamethoxazole by expressing antibiotic resistance genes or by acquiring thymine or thymidine from environmental reservoirs to facilitate DNA synthesis. The purpose of this study was to evaluate whether activated sludge serves as a reservoir for thymine or thymidine, potentially impacting the quantification of antibiotic resistant bacteria. This study also assessed the impacts of varying solids retention time (SRT) on trimethoprim and sulfamethoxazole removal during wastewater treatment and single and multi-drug resistance...
April 26, 2017: Chemosphere
https://www.readbyqxmd.com/read/28493685/selective-detection-of-shiga-like-toxin-1-from-complex-samples-using-pigeon-ovalbumin-functionalized-gold-nanoparticles-as-affinity-probes
#19
Chun-Hsien Li, Yi-Ling Bai, Karuppuchamy Selvaprakash, Kwok-Kong Tony Mong, Yu-Chie Chen
Escherichia coli O157:H7 is a foodborne pathogen. This bacterial strain can generate Shiga-like toxins (SLTs), which can cause serious sickness and even death. Thus, it is important to develop effective and sensitive methods that can be used to rapidly identify the presence of SLTs from complex samples. Pigeon egg white (PEW) contains abundant glycoproteins, including pigeon ovalbumin (POA) (∼60%). POA possesses Gal-α(1→4)-Gal-β(1→4)-GlcNAc termini, which can recognize the B subunits in SLT type 1 (SLT-1B)...
May 31, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28485474/graphene-and-carbon-nanotubes-activate-different-cell-surface-receptors-on-macrophages-before-and-after-deactivation-of-endotoxins
#20
Mohamed H Lahiani, Kuppan Gokulan, Katherine Williams, Mariya V Khodakovskaya, Sangeeta Khare
Nanomaterial synthesis and handling in a non-sterile environment can result in the final product becoming contaminated with bacterial endotoxin or lipopolysaccharides (LPB). During toxicological testing, the effects caused by endotoxin-contaminated nanomaterials can be misinterpreted in the end-point analysis (such as cytotoxicity and immune responses) and could result in erroneous conclusions. The objective of this study was twofold: (i) to test different carbon-based nanomaterials (CBNs) [pristine graphene and multi-wall carbon nanotubes (MWCNTs)] for the presence of endotoxin and develop strategies for depyrogenation, and (ii) to compare the immune response exhibited by macrophages after exposure to native CBNs versus depyrogenated CBNs...
May 9, 2017: Journal of Applied Toxicology: JAT
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