keyword
MENU ▼
Read by QxMD icon Read
search

pich

keyword
https://www.readbyqxmd.com/read/28199320/the-use-of-urinary-proteomics-in-the-assessment-of-suitability-of-mouse-models-for-ageing
#1
Esther Nkuipou-Kenfack, Joost P Schanstra, Seerat Bajwa, Martin Pejchinovski, Claire Vinel, Cédric Dray, Philippe Valet, Jean-Loup Bascands, Antonia Vlahou, Thomas Koeck, Melanie Borries, Hauke Busch, Wibke Bechtel-Walz, Tobias B Huber, Karl L Rudolph, Andreas Pich, Harald Mischak, Petra Zürbig
Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8-96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS)...
2017: PloS One
https://www.readbyqxmd.com/read/28188520/proteome-characterization-of-a-chromatin-locus-using-the-proteomics-of-isolated-chromatin-segments-approach
#2
Sophie L Kan, Nehmé Saksouk, Jérome Déjardin
The biological functions of given genomic regions are ruled by the local chromatin composition. The Proteomics of Isolated Chromatin segments approach (PICh) is a powerful and unbiased method to analyze the composition of chosen chromatin segments, provided they are abundant (repeated) or that the organism studied has a small genome. PICh can be used to identify novel and unexpected regulatory factors, or when combined with quantitative mass spectrometric approaches, to characterize the function of a defined factor at the chosen locus, by quantifying composition changes at the locus upon removal/addition of that factor...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28180981/does-pre-operative-magnetic-resonance-imaging-of-the-lumbar-multifidus-muscle-predict-clinical-outcomes-following-lumbar-spinal-decompression-for-symptomatic-spinal-stenosis
#3
Mario G T Zotti, F Vilas Boas, T Clifton, M Piche, W W Yoon, B J C Freeman
PURPOSE: To investigate whether pre-operative magnetic resonance imaging (MRI) of the lumbar multifidus muscle (LMM) would predict clinical outcomes following lumbar spinal decompression for symptomatic spinal stenosis. METHODS: A prospective cohort of patients with symptomatic neurogenic claudication, documented spinal stenosis on pre-operative MRI underwent spinal decompression. All subjects completed standardised outcome measures (Core Outcome Measures Index (COMI), Oswestry Disability Index (ODI v2...
February 8, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28161310/statistical-power-and-prediction-accuracy-in-multisite-resting-state-fmri-connectivity
#4
Christian Dansereau, Yassine Benhajali, Celine Risterucci, Emilio Merlo Pich, Pierre Orban, Douglas Arnold, Pierre Bellec
Connectivity studies using resting-state functional magnetic resonance imaging are increasingly pooling data acquired at multiple sites. While this may allow investigators to speed up recruitment or increase sample size, multisite studies also potentially introduce systematic biases in connectivity measures across sites. In this work, we measure the inter-site effect in connectivity and its impact on our ability to detect individual and group differences. Our study was based on real, as opposed to simulated, multisite fMRI datasets collected in N=345 young, healthy subjects across 8 scanning sites with 3T scanners and heterogeneous scanning protocols, drawn from the 1000 functional connectome project...
February 2, 2017: NeuroImage
https://www.readbyqxmd.com/read/28139119/proteomic-analysis-of-human-pluripotent-stem-cell-cardiomyogenesis-revealed-altered-expression-of-metabolic-enzymes-and-pdlim5-isoforms
#5
Sarah Anna Konze, Sebastian Werneburg, Astrid Oberbeck, Ruth Olmer, Henning Kempf, Monica Jara-Avaca, Andreas Pich, Robert Zweigerdt, Falk Fritz Reinhold Buettner
Human pluripotent stem cells (hPSCs), both embryonic (hESCs) and induced (hiPSCs), can be differentiated into derivatives of the three germ layers and are promising tools in regenerative medicine. Cardiovascular diseases are the top-ranking cause of premature death worldwide and cell replacement therapies based on in vitro differentiated cardiomyocytes might provide a promising perspective to cure patients in the future. The molecular processes during hPSC cardiomyogenesis are far from being fully understood and we thus have focused here on characterizing the proteome along hESC in vitro differentiation into cardiomyocytes (CMs)...
January 31, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28130344/putting-phds-to-work-phf11-clears-the-way-for-exo1-in-double-strand-break-repair
#6
REVIEW
Karl E Zahn, Roger A Greenberg
In this issue of Genes & Development, Gong and colleagues (pp. 46-58) bring to light a functional role for plant homeodomain finger 11 (PHF11) in 5' end resection at DNA double-strand breaks (DSBs). Using the proteomics of isolated chromatin segments (PICh) technique to purify deprotected telomeres, PHF11 was enriched as cells mounted a DNA damage response (DDR) against exposed chromosome ends. The study reveals interactions between PHF11 and multiple DNA repair proteins and suggests that PHF11 mediates 5' end resection by negotiating RPA-coated DNA repair intermediates...
January 1, 2017: Genes & Development
https://www.readbyqxmd.com/read/28128895/functional-isoeugenol-modified-nanogel-coatings-for-the-design-of-biointerfaces
#7
Michael Kather, Merle Skischus, Pierre Kandt, Andrij Pich, Georg Conrads, Sabine Neuss
A new route for the synthesis of functional aqueous nanogels decorated with a controlled amount of surface-drafted isoeugenol molecules has been developed. Obtained nanogels exhibit two key functions: a) antibacterial activity against different oral pathogens and b) cell-adhesive and -growth-promoting properties. Functional nanogels can be potentially used as building blocks in the design of bioactive coatings on various implants preventing infections and accelerating tissue regeneration.
January 27, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28067034/gadolinium-loaded-poly-n-vinylcaprolactam-nanogels-synthesis-characterization-and-application-for-enhanced-tumor-mr-imaging
#8
Wenjie Sun, Sabrina Thies, Jiulong Zhang, Chen Peng, Guangyu Tang, Mingwu Shen, Andrij Pich, Xiangyang Shi
We report the synthesis of poly(N-vinylcaprolactam) nanogels (PVCL NGs) loaded with gadolinium (Gd) for tumor MR imaging applications. The PVCL NGs were synthesized via precipitation polymerization using the monomer N-vinylcaprolactam (VCL), the comonomer acrylic acid (AAc), and the degradable cross-linker 3,9-divinyl-2,4,8,10-tetraoxaspiro-[5,5]-undecane (VOU) in aqueous solution, followed by covalently binding with 2,2',2″-(10-(4-((2-aminoethyl)amino)-1-carboxy-4-oxobutyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl) triacetic acid (NH2-DOTA-GA)/Gd complexes...
February 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28061761/body-image-satisfaction-and-weight-concerns-among-a-mediterranean-adult-population
#9
Maria Del Mar Bibiloni, Josep Ll Coll, Jordi Pich, Antoni Pons, Josep A Tur
BACKGROUND: People tend to underestimate their current weight and overestimate their height minimizing health risk factors. The aim of this study was to assess body weight satisfaction, acceptance of body image, weight concern and dieting habits among a Mediterranean adult population. METHODS: Cross-sectional survey was carried out (2009-2010) in the Balearic Islands, Spain. A random sample (n = 1081) of young (18-35 y.o.) and middle-aged adults (36-55 y.o.) was interviewed and anthropometrically measured...
January 6, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28051995/in-vitro-modelling-of-familial-amyloidotic-polyneuropathy-allows-quantitative-detection-of-transthyretin-amyloid-fibril-like-structures-in-hepatic-derivatives-of-patient-specific-induced-pluripotent-stem-cells
#10
Jeannine Hoepfner, Mandy Kleinsorge, Oliver Papp, Susanne Alfken, Robin Heiringhoff, Andreas Pich, Vanessa Sauer, Andree Zibert, Gudrun Göhring, Hartmut Schmidt, Malte Sgodda, Tobias Cantz
The transthyretin protein is thermodynamically destabilised by mutations in the transthyretin gene, promoting the formation of amyloid fibrils in various tissues. Consequently, impaired autonomic organ function is observed in patients suffering from transthyretin-related Familial Amyloidotic Polyneuropathy (FAP). The influence of individual genetic backgrounds on fibril formation as a potential cause of genotype-phenotype variations needs to be investigated in order to ensure efficient patient-specific therapies...
January 4, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/28000467/ten-year-trends-2000-2010-in-bias-of-self-reported-weight-height-and-body-mass-index-in-a-mediterranean-adult-population
#11
Maria Del Mar Bibiloni, Josep Ll Coll, Rogelio Salas, Jordi Pich, Antoni Pons, Josep A Tur
:  Aim: To assess trends in the biases of self-reported versus measured weight, height, and body mass index (BMI) in adults over the period 2000-2010 in a Mediterranean adult population. METHODS: The sample population consisted of young (18-35) and middle-aged (36-55) adults living in the Balearic Islands, Spain. The data represent 1,089 people during 1999-2000 and 1,081 people during 2000-2010. Weighted-based frequency estimates were used. RESULTS: While no differences between self-reported and measured weight, height and BMI were found in the respondents, it was seen that a decreasing percentage of the population knows their own weight and/or height...
November 29, 2016: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/27998779/systemic-blood-pressure-alters-cortical-blood-flow-and-neurovascular-coupling-during-nociceptive-processing-in-the-primary-somatosensory-cortex-of-the-rat
#12
Sae Uchida, Suzie Bois, Jean-Paul Guillemot, Hugues Leblond, Mathieu Piché
Inference on nociceptive and pain-related processes from functional magnetic resonance imaging is made with the assumption that the coupling of neuronal activity and cerebral hemodynamic changes is stable. However, since nociceptive stimulation is associated with increases in systemic arterial pressure, it is essential to determine whether this coupling remains the same during different levels of nociception and pain. The main objective of the present study was to compare the amplitude of local field potentials (LFP) and cerebral blood flow (CBF) changes in the primary somatosensory cortex during nociceptive electrical stimulation of the contralateral or ipsilateral forepaw in isoflurane-anesthetized rats, while manipulating mean arterial pressure (MAP)...
December 18, 2016: Neuroscience
https://www.readbyqxmd.com/read/27994808/effects-of-benzo-e-pyrene-on-reactive-oxygen-nitrogen-species-and-inflammatory-cytokines-induction-in-human-rpe-cells-and-attenuation-by-mitochondrial-involved-mechanism
#13
M Fernanda Estrago-Franco, M Tarek Moustafa, Mohammad Riazi-Esfahani, Ashish U Sapkal, Rhina Piche-Lopez, A Jayaprakash Patil, Ashish Sharma, Payam Falatoonzadeh, Marilyn Chwa, Georgia Luczy-Bachman, Baruch D Kuppermann, M Cristina Kenney
PURPOSE: To identify inhibitors that could effectively lower reactive oxygen/nitrogen species (ROS/RNS), complement and inflammatory cytokine levels induced by Benzo(e)pyrene [B(e)p], an element of cigarette smoke, in human retinal pigment epithelial cells (ARPE-19) in vitro. METHODS: ARPE-19 cells were treated for 24 hours with 200 μM, 100 μM, and 50 μM B(e)p or DMSO (dimethyl sulfoxide)-equivalent concentrations. Some cultures were pre-treated with ROS/RNS inhibitors (NG nitro-L-arginine, inhibits nitric oxide synthase; Apocynin, inhibits NADPH oxidase; Rotenone, inhibits mitochondrial complex I; Antimycin A, inhibits mitochondria complex III) and ROS/RNS levels were measured with a fluorescent H2 DCFDA assay...
October 2016: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/27972554/development-of-a-global-model-for-the-economic-evaluation-of-personalized-medicine-for-the-treatment-of-prostate-cancer
#14
Piché-Richard, K Mathurin, M Savoie, J Lachaine
No abstract text is available yet for this article.
November 2016: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27901389/population-pharmacokinetics-of-a-single-dose-of-meloxicam-after-oral-and-intramuscular-administration-to-captive-lesser-flamingos-phoeniconaias-minor
#15
Martín A Zordan, Mark G Papich, Ashley A Pich, Katy M Unger, Carlos R Sánchez
OBJECTIVE To determine the pharmacokinetics of a single dose of meloxicam after IM and oral administration to healthy lesser flamingos (Phoeniconaias minor) by use of a population approach. ANIMALS 16 healthy captive lesser flamingos between 1 and 4 years of age. PROCEDURES A single dose of meloxicam (0.5 mg/kg) was administered IM to each bird, and blood samples were collected from birds at 3 (n = 13 birds), 2 (2), or 1 (1) selected point between 0 and 13 hours after administration, with samples collected from birds at each point...
December 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27899582/the-zebrafish-model-organism-database-new-support-for-human-disease-models-mutation-details-gene-expression-phenotypes-and-searching
#16
Douglas G Howe, Yvonne M Bradford, Anne Eagle, David Fashena, Ken Frazer, Patrick Kalita, Prita Mani, Ryan Martin, Sierra Taylor Moxon, Holly Paddock, Christian Pich, Sridhar Ramachandran, Leyla Ruzicka, Kevin Schaper, Xiang Shao, Amy Singer, Sabrina Toro, Ceri Van Slyke, Monte Westerfield
The Zebrafish Model Organism Database (ZFIN; http://zfin.org) is the central resource for zebrafish (Danio rerio) genetic, genomic, phenotypic and developmental data. ZFIN curators provide expert manual curation and integration of comprehensive data involving zebrafish genes, mutants, transgenic constructs and lines, phenotypes, genotypes, gene expressions, morpholinos, TALENs, CRISPRs, antibodies, anatomical structures, models of human disease and publications. We integrate curated, directly submitted, and collaboratively generated data, making these available to zebrafish research community...
January 4, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27895398/dysregulation-of-innate-immunity-in-ulcerative-colitis-patients-who-fail-anti-tumor-necrosis-factor-therapy
#17
Angela C Baird, Dominic Mallon, Graham Radford-Smith, Julien Boyer, Thierry Piche, Susan L Prescott, Ian C Lawrance, Meri K Tulic
AIM: To study the innate immune function in ulcerative colitis (UC) patients who fail to respond to anti-tumor necrosis factor (TNF) therapy. METHODS: Effects of anti-TNF therapy, inflammation and medications on innate immune function were assessed by measuring peripheral blood mononuclear cell (PBMC) cytokine expression from 18 inflammatory bowel disease patients pre- and 3 mo post-anti-TNF therapy. Toll-like receptor (TLR) expression and cytokine production post TLR stimulation was assessed in UC "responders" (n = 12) and "non-responders" (n = 12) and compared to healthy controls (n = 12)...
November 7, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27860399/toxin-a-of-the-nosocomial-pathogen-clostridium-difficile-induces-primary-effects-in-the-proteome-of-hep-2-cells
#18
Johannes Junemann, Gurbet Birgin, Jelena Erdmann, Anke Schröder, Ingo Just, Ralf Gerhard, Andreas Pich
PURPOSE: This study was carried out to investigate the impact of high concentrations of Clostridium difficile toxin A (TcdA) on the proteome of human cells. It should also be examined whether a catalytically deficient mutant (TcdANXN ) has an effect on target cells. EXPERIMENTAL DESIGN: Proteome changes were investigated after treatment of HEp-2 cells with 20 nM TcdA for 8 h using a triplex SILAC labeling method and shotgun proteomics. Proteins from differently labeled and treated cells were combined for analysis using an HPLC coupled to an Orbitrap mass spectrometer...
November 10, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27850876/1240-improving-recognition-and-management-of-delirium-in-the-medical-intensive-care-unit
#19
Lisa Daniels, John Park, James Skiba, Shannon Piche, Sarah Nelson, Jennifer Elmer, Catherine Bowron
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27849816/173-baseline-thromboelastogram-as-a-predictor-of-left-ventricular-assist-device-thrombosis
#20
Shannon Piche, Scott Nei, Erin Frazee, Sarah Schettle, Barry Boilson, Ross Dierkhising, John Stulak
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
keyword
keyword
79382
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"