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https://www.readbyqxmd.com/read/28623391/survey-of-high-affinity-h2-oxidizing-bacteria-in-soil-reveals-their-vast-diversity-yet-underrepresentation-in-genomic-databases
#1
Sarah Piché-Choquette, Mondher Khdhiri, Philippe Constant
While high-affinity H2-oxidizing bacteria (HA-HOB) serve as the main sink of atmospheric H2, the ecology of this specialist functional group is rather unknown due to its recent discovery. The main purpose of our study is to provide the first extensive survey of HA-HOB in farmland, larch, and poplar soils exposed to 0.5 and 10,000 ppmv H2. Using qPCR and qRT-PCR assays along with PCR amplicon high-throughput sequencing of hhyL gene encoding for the large subunit of high-affinity [NiFe]-hydrogenases (HAH), we found that hhyL gene expression ratio explained better variation in measured H2 oxidation rates than HA-HOB species richness...
June 17, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28612519/glucosyltransferase-dependent-and-independent-effects-of-tcdb-on-the-proteome-of-hep-2-cells
#2
Jelena Erdmann, Johannes Junemann, Anke Schröder, Ingo Just, Ralf Gerhard, Andreas Pich
Toxin B (TcdB) of the nosocomial pathogen C. difficile has been reported to exhibit a glucosyltransferase dependent and independent effect on treated HEp-2 cells at toxin concentration above 0.3 nM. In order to investigate and further characterize both effects epithelial cells were treated with wild type TcdB and glucosyltransferase-deficient TcdBNXN and their proteomes were analyzed by LC-MS. Triplex SILAC labeling was used for quantification. Identification of 5212 and quantification of 4712 protein groups was achieved...
June 14, 2017: Proteomics
https://www.readbyqxmd.com/read/28588781/p53-protein-expression-in-patients-with-myelodysplasia-treated-with-allogeneic-bone-marrow-transplantation
#3
Achille Pich, Laura Godio, Laura Davico Bonino
Tumor protein 53 mutations adversely affect the prognosis of myelodysplastic syndromes (MDS); however, few studies have reported on the prognostic significance of the expression of p53 protein in MDS. The current study investigated p53 immunoreactivity (p53-IR) in bone marrow biopsies (BMBs) obtained at diagnosis from 18 patients (6 females and 12 males; mean age, 50.5 years) with MDS that underwent bone marrow transplantation (BMT) to determine the associations between clinical and histopathological data and outcome...
June 2017: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/28556672/faster-use-and-fewer-failures-with-needle-free-nasal-glucagon-versus-injectable-glucagon-in-severe-hypoglycemia-rescue-a-simulation-study
#4
Jean-François Yale, Hélène Dulude, Marc Egeth, Claude A Piché, Martin Lafontaine, Dolorès Carballo, Rebecca Margolies, Emily Dissinger, Adam R Shames, Nicole Kaplowitz, Michelle Xiaotian Zhang, Shuyu Zhang, Cristina B Guzman
BACKGROUND: During severe hypoglycemic episodes, people with diabetes depend on others to help with treatment. We compared needle-free nasal glucagon and commercially available injectable glucagon for ease of use by caregivers of people with diabetes and by others in treating simulated episodes of severe hypoglycemia. METHODS: Sixteen instructed caregivers and 15 noninstructed acquaintances administered nasal and injectable glucagon to manikins, simulating unconscious people with diabetes during severe hypoglycemia episodes...
May 30, 2017: Diabetes Technology & Therapeutics
https://www.readbyqxmd.com/read/28555408/bariatric-surgery-induced-resolution-of-hypertension-and-obstructive-sleep-apnea-impact-of-modulation-of-body-fat-ectopic-fat-autonomic-nervous-activity-inflammatory-and-adipokine-profiles
#5
Audrey Auclair, Laurent Biertho, Simon Marceau, Frédéric-Simon Hould, Simon Biron, Stéfane Lebel, François Julien, Odette Lescelleur, Yves Lacasse, Marie-Eve Piché, Katherine Cianflone, Sebastian Demian Parlee, Kerry Goralski, Julie Martin, Marjorie Bastien, David H St-Pierre, Paul Poirier
BACKGROUND: Obesity-associated systemic hypertension (HTN) and obstructive sleep apnea (OSA) have multiple pathophysiological pathways including ectopic fat deposition, inflammation, altered adipokine profile, and increased sympathetic nervous activity. We characterized these potential mechanisms in severely obese patients with or without HTN and OSA. We also compared changes of these mechanisms at 12 months following biliopancreatic diversion with duodenal switch (BPD-DS) surgery according to HTN and OSA resolution...
May 29, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28551583/evaluation-of-toxic-and-genotoxic-potential-of-a-wet-gas-scrubber-effluent-obtained-from-wooden-based-biomass-furnaces-a-case-study-in-the-red-ceramic-industry-in-southern-brazil
#6
Tiago Bortolotto, Jaqueline da Silva, Alex Célio Sant'Ana, Kamila Osowski Tomazi, Reginaldo Geremias, Elídio Angioletto, Claus Tröger Pich
Red ceramic industry in southern Brazil commonly uses wood biomass as furnace fuel generating great amounts of gas emissions and ash. To avoid their impact on atmospheric environment, wet scrubbing is currently being applied in several plants. However, the water leachate formed could be potentially toxic and not managed as a common water-based effluent, since the resulting wastewater could carry many toxic compounds derived from wood pyrolysis. There is a lack of studies regarding this kind of effluent obtained specifically and strictly from wooden-based biomass furnaces...
May 25, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28542481/effect-of-hyperbaric-oxygen-on-bdnf-release-and-neuroprotection-investigations-with-human-mesenchymal-stem-cells-and-genetically-modified-nih3t3-fibroblasts-as-putative-cell-therapeutics
#7
Jennifer Schulze, Odett Kaiser, Gerrit Paasche, Hans Lamm, Andreas Pich, Andrea Hoffmann, Thomas Lenarz, Athanasia Warnecke
Hyperbaric oxygen therapy (HBOT) is a noninvasive widely applied treatment that increases the oxygen pressure in tissues. In cochlear implant (CI) research, intracochlear application of neurotrophic factors (NTFs) is able to improve survival of spiral ganglion neurons (SGN) after deafness. Cell-based delivery of NTFs such as brain-derived neurotrophic factor (BDNF) may be realized by cell-coating of the surface of the CI electrode. Human mesenchymal stem cells (MSC) secrete a variety of different neurotrophic factors and may be used for the development of a biohybrid electrode in order to release endogenously-derived neuroprotective factors for the protection of residual SGN and for a guided outgrowth of dendrites in the direction of the CI electrode...
2017: PloS One
https://www.readbyqxmd.com/read/28529633/noninvasive-assessment-of-elimination-and-retention-using-ct-fmt-and-kinetic-whole-body-modeling
#8
Wa'el Al Rawashdeh, Simin Zuo, Andrea Melle, Lia Appold, Susanne Koletnik, Yoanna Tsvetkova, Nataliia Beztsinna, Andrij Pich, Twan Lammers, Fabian Kiessling, Felix Gremse
Fluorescence-mediated tomography (FMT) is a quantitative three-dimensional imaging technique for preclinical research applications. The combination with micro-computed tomography (µCT) enables improved reconstruction and analysis. The aim of this study is to assess the potential of µCT-FMT and kinetic modeling to determine elimination and retention of typical model drugs and drug delivery systems. We selected four fluorescent probes with different but well-known biodistribution and elimination routes: Indocyanine green (ICG), hydroxyapatite-binding OsteoSense (OS), biodegradable nanogels (NG) and microbubbles (MB)...
2017: Theranostics
https://www.readbyqxmd.com/read/28524364/biofunctional-microgel-based-fertilizers-for-controlled-foliar-delivery-of-nutrients-to-plants
#9
Richard A Meurer, Steffen Kemper, Sandra Knopp, Thomas Eichert, Felix Jakob, Heiner E Goldbach, Ulrich Schwaneberg, Andrij Pich
Foliar application of micronutrients (e.g. Fe(3+) ) onto plants over an extended time is challenging and often not possible due to insufficient rainfastness. Smart delivery systems which enable micronutrient release over several weeks would offer innovative and sustainable options to improve plant health and food production. Herein, we report a novel foliar fertilizer delivery system based on functional pH-responsive biohybrid microgels that have orthogonal functionality as carriers of micronutrients and employ peptides (termed anchor peptides) as foliar adhesion promoters...
May 19, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28514834/antitumor-activity-of-brazilian-red-propolis-fractions-against-hep-2-cancer-cell-line
#10
Caroline Olivieri da Silva Frozza, Denis Amilton Santos, Luciane Corbellini Rufatto, Luciane Minetto, Fernando Joel Scariot, Sergio Echeverrigaray, Claus Tröger Pich, Sidnei Moura, Francine Ferreira Padilha, Sibele Borsuk, Lucielli Savegnago, Tiago Collares, Fabiana Kömmling Seixas, Odir Dellagostin, Mariana Roesch-Ely, João Antonio Pêgas Henriques
Continuous increases in the rates of tumor diseases have highlighted the need for identification of novel and inexpensive antitumor agents from natural sources. In this study, we investigated the effects of enriched fraction from hydroalcoholic Brazilian red propolis extract against Hep-2 cancer cell line. Initially 201 fractions were arranged in 12 groups according to their chromatographic characteristics (A-L). After an in vitro cell viability screening, J and L were further selected as promising enriched fractions for this study...
July 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28509371/quantitative-assessment-of-sialo-glycoproteins-and-n-glycans-during-cardiomyogenic-differentiation-of-human-induced-pluripotent-stem-cells
#11
Sarah A Konze, Samanta Cajic, Astrid Oberbeck, René Hennig, Andreas Pich, Erdmann Rapp, Falk F R Buettner
Human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC CMs) may be used in regenerative medicine for individualized tissue transplants in the future. For application in patients, the generated CMs have to be highly pure and well characterized. In order to overcome the prevalent scarcity of CM-specific markers, we quantitatively assessed cell-surface-exposed sialo-glycoproteins and N-glycans of hiPSCs, CM progenitors, and CMs. Applying a combination of metabolic labeling and specific sialo-glycoprotein capture, we could highly enrich and quantify membrane proteins during cardiomyogenic differentiation...
May 16, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28479402/tight-neurovascular-coupling-in-the-spinal-cord-during-nociceptive-stimulation-in-intact-and-spinal-rats
#12
Mathieu Piché, Thierry Paquette, Hugues Leblond
Functional magnetic resonance imaging (fMRI) is based on neurovascular coupling, which allows inferring neuronal activity from hemodynamic changes. Spinal fMRI has been used to examine pain processes, although spinal neurovascular coupling has never been investigated. In addition, fluctuations in mean arterial pressure (MAP) occur during nociceptive stimulation and this may affect neurovascular coupling. The objective of this study was to examine neurovascular coupling in the rat spinal cord during nociceptive stimulation while MAP was manipulated by cervical spinal transection, which prevents nociception-related MAP increases...
May 4, 2017: Neuroscience
https://www.readbyqxmd.com/read/28476758/poststroke-epilepsy-in-long-term-survivors-of-primary-intracerebral-hemorrhage
#13
Anna-Maija Lahti, Pertti Saloheimo, Juha Huhtakangas, Henrik Salminen, Seppo Juvela, Michaela K Bode, Matti Hillbom, Sami Tetri
OBJECTIVE: To identify the incidence and predisposing factors for development of poststroke epilepsy (PSE) after primary intracerebral hemorrhage (PICH) during a long-term follow-up. METHODS: We performed a retrospective study of patients who had had their first-ever PICH between January 1993 and January 2008 in Northern Ostrobothnia, Finland, and who survived for at least 3 months. These patients were followed up for PSE. The associations between PSE occurrence and sex, age, Glasgow Coma Scale (GCS) score on admission, hematoma location and volume, early seizures, and other possible risk factors for PSE were assessed using the Cox proportional hazards regression model...
June 6, 2017: Neurology
https://www.readbyqxmd.com/read/28439809/site-selection-a-case-study-in-the-identification-of-optimal-cysteine-engineered-antibody-drug-conjugates
#14
L Nathan Tumey, Fengping Li, Brian Rago, Xiaogang Han, Frank Loganzo, Sylvia Musto, Edmund I Graziani, Sujiet Puthenveetil, Jeffrey Casavant, Kimberly Marquette, Tracey Clark, Jack Bikker, Eric M Bennett, Frank Barletta, Nicole Piche-Nicholas, Amy Tam, Christopher J O'Donnell, Hans Peter Gerber, Lioudmila Tchistiakova
As the antibody drug conjugate (ADC) community continues to shift towards site-specific conjugation technology, there is a growing need to understand how the site of conjugation impacts the biophysical and biological properties of an ADC. In order to address this need, we prepared a carefully selected series of engineered cysteine ADCs and proceeded to systematically evaluate their potency, stability, and PK exposure. The site of conjugation did not have a significant influence on the thermal stability and in vitro cytotoxicity of the ADCs...
April 24, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28389378/sympathetic-regulation-and-anterior-cingulate-cortex-volume-are-altered-in-a-rat-model-of-chronic-back-pain
#15
Sara Touj, Sébastien Houle, Djamel Ramla, Renaud Jeffrey-Gauthier, Harumi Hotta, Gilles Bronchti, Maria-Grazia Martinoli, Mathieu Piché
Chronic pain is associated with autonomic disturbance. However, specific effects of chronic back pain on sympathetic regulation remain unknown. Chronic pain is also associated with structural changes in the anterior cingulate cortex (ACC), which may be linked to sympathetic dysregulation. The aim of this study was to determine whether sympathetic regulation and ACC surface and volume are affected in a rat model of chronic back pain, in which complete Freund Adjuvant (CFA) is injected in back muscles. Sympathetic regulation was assessed with renal blood flow (RBF) changes induced by electrical stimulation of a hind paw, while ACC structure was examined by measuring cortical surface and volume...
June 3, 2017: Neuroscience
https://www.readbyqxmd.com/read/28371270/physicochemical-characterization-of-the-shell-composition-of-pbca-based-polymeric-microbubbles
#16
Lia Appold, Yang Shi, Stephan Rütten, Alexander Kühne, Andrij Pich, Fabian Kiessling, Twan Lammers
Microbubbles (MB) are routinely used as contrast agents for ultrasound (US) imaging. In recent years, MB have also attracted interest as drug delivery systems. Soft-shelled lipidic MB tend to be more advantageous for US imaging, while hard-shelled polymeric MB appear to be more suitable for drug delivery purposes because of their thicker shell and the resulting higher drug loading capacity. The physicochemical composition of the shell of polymeric MB, however, remains largely unknown. This study sets out to evaluate the molecular weight and polydispersity of the building blocks constituting the shell of poly(butyl cyanoacrylate) (PBCA) MB...
March 29, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28367369/molecular-barcoding-confirms-the-presence-of-exotic-asian-seaweeds-pachymeniopsis-gargiuli-and-grateloupia-turuturu-in-the-cantabrian-sea-bay-of-biscay
#17
Marcos Montes, Jose M Rico, Eva García-Vazquez, Yaisel J Borrell Pichs
BACKGROUND: The introduction of exotic species can have serious consequences for marine ecosystems. On the shores of the Cantabrian Sea (North of Spain) there are no routine examinations of seaweeds that combine molecular and morphological methods for early detection of exotic species making it difficult to assess in the early stages their establishment and expansion processes as a result of anthropogenic activities (e.g., shipping and/or aquaculture). METHODS: In this work we used both morphological identification and molecular barcoding (COI-5P and rbcL genes) of red algae collected in Asturias, Bay of Biscay (Gijón and Candás harbours) and from the University of Oviedo's herbarium samples...
2017: PeerJ
https://www.readbyqxmd.com/read/28363961/the-tale-of-a-neglected-energy-source-elevated-hydrogen-exposure-affects-both-microbial-diversity-and-function-in-soil
#18
Mondher Khdhiri, Sarah Piché-Choquette, Julien Tremblay, Susannah G Tringe, Philippe Constant
The enrichment of H2-oxidizing bacteria (HOB) by H2 generated by nitrogen-fixing nodules has been shown to have a fertilization effect on several different crops. The benefit of HOB is attributed to their production of plant growth-promoting factors, yet their interactions with other members of soil microbial communities have received little attention. Here we report that the energy potential of H2, when supplied to soil, alters ecological niche partitioning of bacteria and fungi, with multifaceted consequences for both generalist and specialist microbial functions...
June 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28303507/laparoscopic-conversion-of-one-anastomosis-gastric-bypass-to-a-standard-roux-en-y-gastric-bypass
#19
Imed Ben Amor, Niccolo Petrucciani, Radwan Kassir, Abdullah Al Munifi, Thierry Piche, Tarek Debs, Jean Gugenheim
INTRODUCTION: One anastomosis gastric bypass (OAGB) demonstrated results similar to traditional Roux-en-Y procedures [1-3], in terms of weight loss and resolution of obesity-related comorbidities. The main controversy regarding OAGB is the concern for an association between biliary alkaline gastritis and esophageal or gastric cancer raised by some studies [4]. METHODS: We present the case of a 51-year-old woman with a BMI of 41 kg/m2 who underwent a laparoscopic OAGB in 2014...
March 16, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28288900/the-mechanism-of-back-pain-relief-by-spinal-manipulation-relies-on-decreased-temporal-summation-of-pain
#20
Christopher Randoll, Vincent Gagnon-Normandin, Jessica Tessier, Suzie Bois, Nabi Rustamov, Julie O'Shaughnessy, Martin Descarreaux, Mathieu Piché
The aim of the present study was to determine whether thoracic spinal manipulation (SM) decreases temporal summation of back pain. The study comprised two controlled experiments including 16 and 15 healthy participants, respectively. Each study included six sessions during which painful or non-painful electrical stimulations were delivered in three conditions: (1) control (2) light mechanical stimulus (MS) or (3) SM. Electrical stimulation was applied on the thoracic spine (T4), in the area where SM and MS were performed...
May 4, 2017: Neuroscience
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