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cornea transplantation

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https://www.readbyqxmd.com/read/27914026/limbal-stem-cell-and-oral-mucosal-epithelial-transplantation-from-ex-vivo-cultivation-in-lscd-induced-rabbits-histology-and-immunologic-study-of-the-transplant-epithelial-sheet
#1
Napaporn Tananuvat, Kanokkan Bumroongkit, Chainarong Tocharusa, Umnat Mevatee, Aphisek Kongkaew, Somsanguan Ausayakhun
PURPOSE: To evaluate the results of cultivated limbal epithelial and oral mucosal epithelial transplantation (CLET and COMET) in limbal stem cell deficiency (LSCD)-induced rabbit model. MATERIALS AND METHODS: Six New Zealand white rabbits were divided into two groups of three rabbits each. Limbal tissue was harvested from the first group, and oral mucosal biopsy was obtained from the second group. The tissues were cultured using an explant technique with amniotic membrane as a substrate and co-culture with the 3T3 fibroblast and air-lifting method...
December 2, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27907157/human-bone-derived-collagen-for-the-development-of-an-artificial-corneal-endothelial-graft-in-vivo-results-in-a-rabbit-model
#2
Natalia Vázquez, Manuel Chacón, Carlos A Rodríguez-Barrientos, Jesús Merayo-Lloves, Miguel Naveiras, Begoña Baamonde, Jose F Alfonso, Iriana Zambrano-Andazol, Ana C Riestra, Álvaro Meana
Corneal keratoplasty (penetrating or lamellar) using cadaveric human tissue, is nowadays the main treatment for corneal endotelial dysfunctions. However, there is a worldwide shortage of donor corneas available for transplantation and about 53% of the world's population have no access to corneal transplantation. Generating a complete cornea by tissue engineering is still a tough goal, but an endothelial lamellar graft might be an easier task. In this study, we developed a tissue engineered corneal endothelium by culturing human corneal endothelial cells on a human purified type I collagen membrane...
2016: PloS One
https://www.readbyqxmd.com/read/27900926/-corneal-cross-linking-as-a-treatment-for-progressive-keratoconus
#3
R P L Wisse, N Soeters, D A Godefrooij, N G de Koning-Tahzib
Keratoconus is a corneal disease with onset typically occurring during puberty or early adulthood. The cornea progressively thins and acquires a cone-like shape which negatively affects visual acuity. In the early stages, visual acuity can be corrected with glasses or contact lenses. In more advanced cases, a corneal transplant is ultimately indicated to restore visual acuity. Corneal cross-linking (CXL) is a treatment given at a relatively early stage that protects patients against deterioration of visual acuity and further corneal deformation in progressive cases of keratoconus...
2016: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/27888184/amnion-assisted-conjunctival-epithelial-redirection-in-limbal-stem-cell-grafting
#4
Harminder S Dua, Ammar Miri, Mohamed S Elalfy, Anna Lencova, Dalia G Said
AIMS: To develop a technique using amniotic membrane (AM) to prevent admixture of conjunctival epithelial cells and limbal explant-derived corneal epithelial cells in patients undergoing limbal stem cell transplantation. To compare this technique with the current method of 'sequential sector conjunctival epitheliectomy' (SSCE). METHODS: 26 patients with total limbal stem cell deficiency who underwent ocular surface reconstruction with limbal stem cells transplantation were retrospectively studied...
November 25, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27873063/-a%C3%A2-new-technique-to-facilitate-donor-preparation-for-dmek-surgery
#5
P W Rieck, T Engels
BACKGROUND: Descemet membrane endothelial keratoplasty (DMEK) is becoming more and more the method of choice to treat corneal endothelial diseases in specialized centers. The reasons that prevent this technique from becoming widespread are the delicate donor tissue preparation. By inverting the curvature of the cornea from convex to concave after mounting onto an artificial anterior chamber, we developed a combined manual delamination and hydrodissection technique, which allows a rapid and endothelium-preserving method of separating donor Descemet membranes from the underlying stroma...
November 21, 2016: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/27861306/enhancing-dmek-success-by-identifying-optimal-levels-of-trypan-blue-dye-application-to-donor-corneal-tissue
#6
Parag A Majmudar, Lauren Johnson
PURPOSE: The purpose of this study was to determine the correct concentration and duration of application of trypan blue dye to Descemet membrane endothelial keratoplasty (DMEK) donor corneal tissue before transplant to enhance visibility while minimizing damage to the tissue. METHODS: Corneas were precut for DMEK using an 8-mm punch, and each was stained with either 0.06% or 0.15% trypan blue for a duration of 1, 3, or 5 minutes. After staining, each tissue was placed in balanced salt solution and observed to determine how quickly the stain faded from the tissue using a grading system...
November 16, 2016: Cornea
https://www.readbyqxmd.com/read/27853009/outcome-of-transplanted-donor-corneas-with-more-than-6-h-of-death-to-preservation-time
#7
Ashik Mohamed, Sunita Chaurasia, Prashant Garg
PURPOSE: In tropical countries, physicians are skeptic in using corneas with death-to-preservation time (DTPT) >6 h, concerns being endothelial cell viability and microbial contamination on prolonged DTPT. The objective of the study was to investigate these concerns by analyzing the outcomes of corneal transplants performed using donor corneas with DTPT >6 h. MATERIALS AND METHODS: The study was a retrospective case series of 65 transplants performed in 2013 with donor corneas that had DTPT >6 h (range, 6...
September 2016: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/27849309/activation-of-rac1-and-rhoa-preserve-corneal-endothelial-barrier-function
#8
María C Ortega, Diana Santander-García, Beatriz Marcos-Ramiro, Susana Barroso, Susan Cox, Ignacio Jiménez-Alfaro, Jaime Millán
Purpose: The corneal endothelium is responsible for the correct hydration of the corneal stroma. Corneal endothelial cells have a low proliferative capacity, so preserving their barrier function under suboptimal conditions that cause osmotic imbalance, such as those arising from corneal pathologies, age, cryopreservation, and transplantation, is essential for maintaining corneal transparency. We have investigated the signaling induced by hyperosmotic shock that reversibly disrupts corneal endothelial barriers in human endothelial cells and in murine corneas...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27843236/mycobacterium-chelonae-in-a-tectonic-corneal-graft
#9
Haziq Raees Chowdhury, Oliver Comyn, Gill Jones, Mayank A Nanavaty
Atypical mycobacterial infections of the cornea can present with nonspecific inflammatory changes and graft rejection, with no obvious focus to culture and a subsequent delay to diagnosis. These pathogens are well documented in the literature following laser-assisted in situ keratomileusis but have rarely been described following corneal transplant surgery. We report a single case of Mycobacterium chelonae keratitis 1 year after tectonic keratoplasty.
September 2016: Oman Journal of Ophthalmology
https://www.readbyqxmd.com/read/27832940/concomitant-simple-limbal-epithelial-transplantation-after-surgical-excision-of-ocular-surface-squamous-neoplasia
#10
Swathi Kaliki, Faraz Ali Mohammad, Prerana Tahiliani, Virender S Sangwan
PURPOSE: To compare the surgical outcomes of ocular surface squamous neoplasia (OSSN) following wide excisional biopsy with and without primary simple limbal epithelial transplantation (p-SLET). DESIGN: Non-randomized clinical study with historical controls METHODS: Setting: Single-institutional study PATIENTS: 8 patients who underwent wide excisional biopsy of OSSN without p-SLET (historical controls) and 7 patients with p-SLET (cases) INTERVENTION: Wide excisional biopsy, p-SLET MAIN OUTCOME MEASURES: Limbal stem cell deficiency (LSCD) RESULTS: The tumor features between cases versus historical controls including mean number of limbal clock hours affected by OSSN (6 vs 4; p=0...
November 7, 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27831562/mitochondrial-transfer-of-mesenchymal-stem-cells-effectively-protects-corneal-epithelial-cells-from-mitochondrial-damage
#11
Dan Jiang, Fei Gao, Yuelin Zhang, David Sai Hung Wong, Qing Li, Hung-Fat Tse, Goufeng Xu, Zhendong Yu, Qizhou Lian
Recent studies have demonstrated that mesenchymal stem cells (MSCs) can donate mitochondria to airway epithelial cells and rescue mitochondrial damage in lung injury. We sought to determine whether MSCs could donate mitochondria and protect against oxidative stress-induced mitochondrial dysfunction in the cornea. Co-culturing of MSCs and corneal epithelial cells (CECs) indicated that the efficiency of mitochondrial transfer from MSCs to CECs was enhanced by Rotenone (Rot)-induced oxidative stress. The efficient mitochondrial transfer was associated with increased formation of tunneling nanotubes (TNTs) between MSCs and CECs, tubular connections that allowed direct intercellular communication...
November 10, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27829382/corneal-keloid-four-case-reports-of-clinicopathological-features-and-surgical-outcome
#12
Hyo Kyung Lee, Hyuk Jin Choi, Mee Kum Kim, Won Ryang Wee, Joo Youn Oh
BACKGROUND: Surgical outcome of corneal keloid is largely variable depending on reports, although surgical management is inevitable in visually significant cases. We here report clinical features, histopathological findings, and surgical outcome of four cases of corneal keloid. CASE PRESENTATION: Four Korean male patients without a history of corneal trauma or disease were clinically and histologically evaluated for a slowly-growing, white opacity in the cornea...
November 9, 2016: BMC Ophthalmology
https://www.readbyqxmd.com/read/27824384/refractive-lenticule-transplantation-for-correction-of-iatrogenic-hyperopia-and-high-astigmatism-after-lasik
#13
Apostolos Lazaridis, Dan Z Reinstein, Timothy J Archer, Stephan Schulze, Walter Sekundo
PURPOSE: To design a technique for intrastromal transplantation of stromal lenticules with specific refractive power for correction of post-LASIK induced hyperopia and astigmatism. METHODS: A 28-year-old patient was referred for consultation after complicated LASIK for moderate myopia and astigmatism. The refractive error of the right eye was severely overcorrected due to data entry error. Post-LASIK refraction showed high astigmatism (right eye: +6.50 -9.00 @ 84°) and corrected distance visual acuity (CDVA) of 20/32...
November 1, 2016: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/27819779/-quality-of-corneal-tissue-procured-by-trained-non-ophthalmologist-medical-personnel
#14
Karla Verdiguel-Sotelo, Arturo Carrasco-Quiroz, Paul Alejandro López-Macías, Roberto Ortiz-Lerma
BACKGROUND: The cornea is the first tissue awaiting transplantation at a national level. It is imperative to train health personnel in procurement technique with excision of corneoscleral button in situ. The objective was to compare the quality of corneal tissue procured by social service medical interns and Ophthalmology residents in a third level hospital. METHODS: We performed a quasi-experimental pilot study with two groups: group 1, which included trained social service medical interns, and group 2, which included trained residents of Ophthalmology...
November 2016: Revista Médica del Instituto Mexicano del Seguro Social
https://www.readbyqxmd.com/read/27811563/systemic-fungal-infections-in-donors-for-corneal-transplantation
#15
Sheila Pabon, Thomas D Lindquist, Thomas D Miller, Bennie H Jeng
PURPOSE: To determine the prevalence of postkeratoplasty fungal infection when corneal tissue from donors with a recent medical history of oral thrush or respiratory, urine, wound, sputum, bronchial, tracheal, or throat culture positive for fungus is identified before recovery and after decontamination of the corneal tissue with 5% povidone-iodine flush to the donors' eyes during recovery. METHODS: This is a prospective analysis of corneas from 42 donors with a documented medical history of fungus or positive fungal culture, which were recovered between January 2010 and November 2010...
November 2, 2016: Cornea
https://www.readbyqxmd.com/read/27806381/integrin-alpha-9-blockade-suppresses-lymphatic-valve-formation-and-promotes-transplant-survival
#16
Gyeong Jin Kang, Tan Truong, Eric Huang, Valerie Su, Shaokui Ge, Lu Chen
Purpose: The lymphatic pathway mediates transplant rejection. We recently reported that lymphatic vessels develop luminal valves in the cornea during lymphangiogenesis, and these valves express integrin alpha 9 (Itga-9) and play a critical role in directing lymph flow. In this study, we used an allogeneic corneal transplantation model to investigate whether Itga-9 blockade could suppress valvulogenesis after transplantation, and how this effect would influence the outcomes of the transplants...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27792791/derivation-of-corneal-keratocyte-like-cells-from-human-induced-pluripotent-stem-cells
#17
Richard W Naylor, Charles N J McGhee, Chad A Cowan, Alan J Davidson, Teresa M Holm, Trevor Sherwin
Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based therapies may offer an alternative approach given that the eye is amenable to such treatments and corneal diseases like keratoconus have been associated specifically with the death of corneal keratocytes. The ability to generate corneal keratocytes in vitro may enable a cell-based therapy to treat patients with keratoconus...
2016: PloS One
https://www.readbyqxmd.com/read/27785556/-influence-of-the-donor-age-on-graft-survival-is-the-demographic-change-also-important-for-corneal-tissue-donation
#18
REVIEW
N Hofmann, M Derks, M Börgel, K Engelmann
The success of corneal transplantation highly depends on the quality of the used graft. Various factors play a crucial role such as a perfect stroma without optically relevant, centrally located cloudiness or changes (e. g. scars) or an adherent Descemet membrane. One of the most important parameters is the quality of the endothelial cell layer with a sufficiently large endothelial cell count. An open question is so far whether the donor age affects corneal quality and therefore has an impact on the success of transplantation...
October 26, 2016: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/27777123/descemet-membrane-adhesion-strength-is-greater-in-diabetics-with-advanced-disease-compared-to-healthy-donor-corneas
#19
Chaid Schwarz, Benjamin T Aldrich, Kimberlee A Burckart, Gregory A Schmidt, M Bridget Zimmerman, Cynthia R Reed, Mark A Greiner, Edward A Sander
Descemet membrane endothelial keratoplasty (DMEK) is an increasingly popular surgical procedure for treating ocular diseases that require a corneal transplant. Previous studies have found that tissue tearing during surgical preparation is more likely elevated in eyes from donors with a history of diabetes mellitus. To quantify these potential differences, we established an experimental technique for quantifying the force required to separate the endothelium-Descemet membrane complex (EDM) from stroma in human donor corneal tissue, and we assessed differences in adhesion strength between diabetic and non-diabetic donor corneas...
October 21, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27768117/long-term-results-of-allogenic-penetrating-limbo-keratoplasty-20-years-of-experience
#20
S J Lang, D Böhringer, G Geerling, T Reinhard
AimThe objective of the study was to evaluate the long-term results of allogenic penetrating limbo-keratoplasy. This method allows simultaneous transplantation of a corneal graft and limbal stem cells of the donor by means of eccentric trephination of the donor button.MethodThe data of 192 consecutive cases of allogenic penetrating limbo-keratoplasty from 1995 to 2015 were reviewed. These had been performed exclusively in eyes with complete failure of the limbal stem cells, in combination with deep corneal scarring...
October 21, 2016: Eye
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