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cornea transplantation

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https://www.readbyqxmd.com/read/29656016/riding-the-cell-jamming-boundary-geometry-topology-and-phase-of-human-corneal-endothelium
#1
Nigel H Brookes
It is important to assess the viability of eye-banked corneas prior to transplantation due to inherent senescence and known loss of endothelial cells during surgical manipulation. Corneal endothelial cells have a complex basal and paracellular shape making them challenging to accurately measure, particularly in oedematous ex vivo tissue. This study used calibrated centroidal Voronoi Diagrams to segment cells in images of these human corneas, in order to characterize endothelial geometry, topology, and phase...
April 12, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29652516/attitudes-and-perception-towards-eye-donation-in-patients-with-corneal-disease-a-case-controlled-population-based-study
#2
Gupta Noopur, Vashist Praveen, Tandon Radhika, Gupta Sanjeev K, Kalaivani Mani, Kumar Deepak
PURPOSE: To assess awareness, barriers, and misconceptions related to eye donation in people with corneal disease as compared to controls in a population setting. MATERIALS AND METHODS: A population-based study was conducted in 25 randomly selected clusters of Rural Gurgaon, Haryana, India, as part of the CORE (Cornea Opacity Rural Epidemiological) study. In addition to ophthalmic examination, knowledge and perceptions regarding eye donation were assessed through a validated questionnaire...
April 13, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29625470/human-conjunctival-epithelial-sheets-grown-on-poly-lactic-co-glycolic-acid-membranes-and-cocultured-with-human-tenon-s-fibroblasts-for-corneal-repair
#3
Soyoung Hong, Je Hwan Yun, Eun-Soon Kim, Ji Seon Kim, Hungwon Tchah, Changmo Hwang
Purpose: We determine the feasibility of human conjunctival epithelial cells (hCjECs) on poly(lactic-co-glycolic) acid (PLGA) membranes for corneal epithelium regeneration. hCjECs on PLGA or polyester (PET) membranes with or without coculture of human Tenon's fibroblasts (hTFs) were compared in vitro, and to determine whether epithelial sheets grown on PLGA membranes can repair injured rabbit corneal epithelium by transplantation for 2 weeks in vivo. Methods: Primary hCjECs were cultured on PLGA or the original PET membrane-based transwell inserts with or without coculture of hTFs on the floor of the culture plate...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29617399/activation-of-human-macrophages-by-human-corneal-allogen-in-vitro
#4
Paola Kammrath Betancor, Antonia Hildebrand, Daniel Böhringer, Florian Emmerich, Günther Schlunck, Thomas Reinhard, Thabo Lapp
PURPOSE: To study distinct aspects of human monocyte-derived macrophage (MDM) activation by human corneal tissue as a possible initial stage in human corneal allograft rejection. METHODS: Human monocytes were isolated from peripheral blood mononuclear cells (PBMC) and differentiated into MDM. Human corneas with or without endothelium were fragmented using a standardized protocol. MDM were stimulated with human corneal fragments, corneal fragment supernatant, lipopolysaccharide (LPS) or interferon-gamma (IFNγ), and expression profiles for 34 cytokines were determined in MDM-conditioned media using a Luminex bead-based multiplex assay...
2018: PloS One
https://www.readbyqxmd.com/read/29615834/corneal-clarity-measurements-in-patients-with-keratoconus-undergoing-either-penetrating-or-deep-anterior-lamellar-keratoplasty
#5
Khaled Alzahrani, Syarifah-Faiza Dardin, Fiona Carley, Arun Brahma, Debbie Morley, M Chantal Hillarby
Purpose: To compare the corneal clarity measurement between penetrating keratoplasty (PK) and deep anterior lamellar keratoplasty (DALK) in patients with keratoconus, using densitometry software for the Oculus Pentacam. Methods: A retrospective comparative study was carried out at Manchester Royal Eye Hospital. Data were collected 12-18 months after corneal transplantation for keratoconus, including postoperative corneal densitometry, best corrected visual acuity (BCVA), central corneal thickness (CCT), and other relevant clinical details...
2018: Clinical Ophthalmology
https://www.readbyqxmd.com/read/29610174/recalcitrant-atopic-keratoconjunctivitis-in-children-a-case-report-and-literature-review
#6
Jing Li, Xiaohui Luo, Hongmin Ke, Lingyi Liang
Atopic keratoconjunctivitis (AKC) is the most severe type of allergic conjunctivitis and may eventually lead to blindness. Although AKC is reported to be more prevalent in adults, we report a child with AKC whose clinical characteristics were not inconsistent with those typically seen in adult patients with AKC, and who was refractory to traditional topical anti-inflammatory and immunosuppressant therapies. An 11-year-old boy presented with a 3-month history of ocular redness and itching and decreased vision for a week in both eyes...
April 2018: Pediatrics
https://www.readbyqxmd.com/read/29595764/use-of-donor-corneas-from-pseudophakic-eyes-for-descemet-membrane-endothelial-keratoplasty
#7
Thabo Lapp, Sonja Heinzelmann, Daniel Böhringer, Philipp Eberwein, Thomas Reinhard, Philip Maier
PURPOSE: The prevalence of pseudophakia increases with age and affects more than 30% of people older than 80 years. Because grafts from older donors seem to be more suitable for Descemet membrane endothelial keratoplasty (DMEK) surgery rather than grafts from younger donors, eye bank technicians and corneal surgeons have to deal with corneas from pseudophakic donors for DMEK graft preparation. However, graft preparation from corneas of pseudophakic donors can be more complicated. METHODS: We retrospectively analyzed our donor registry for the prevalence of pseudophakia...
March 27, 2018: Cornea
https://www.readbyqxmd.com/read/29595762/transplantation-blues-inadvertent-staining-of-amyloid-deposits-with-trypan-blue
#8
Agathe Marcon, Nicolas Périllat, Thibaud Garcin, Rémy Jullienne, Zhiguo He, Fabien Forest, Anne Sophie Gauthier, Philippe Gain, Gilles Thuret
PURPOSE: To describe inadvertent persistent staining of stromal amyloid deposits by trypan blue (TB) after penetrating keratoplasty (PK) and Descemet membrane endothelial keratoplasty (DMEK) performed in patients with corneal amyloidosis. METHODS: Case series of patients with corneal amyloidosis in whom intraoperative TB was used. RESULTS: One patient, hospitalized for acute rejection 6 weeks after DMEK, presented with an intense blue staining of small, spindle-shaped structures in the anterior half of the cornea...
March 27, 2018: Cornea
https://www.readbyqxmd.com/read/29579837/corneal-donor-profile-and-evolution-of-corneal-donation-in-a-brazilian-state-where-the-number-of-individuals-on-the-waiting-list-reached-zero-but-increased-again
#9
L S M Freitas, P C Rocon, A V de Almeida, R G N Erlacher, F M Paro
BACKGROUND: In most countries, the amount of corneal graft tissue available for transplantation is insufficient to meet demand. In 2010, there were 459 patients on the waiting list for corneal transplantation (WLCT) in Espirito Santo (ES), a Brazilian state with 3,885,000 inhabitants. Several strategies were implemented to reduce the number of individuals on the WLCT, which reached zero in 2011. Studies on the evolution of corneal donation contribute to reducing the WLCT worldwide. The aim of this study was to describe the evolution of corneal donation in ES and the profile of corneal donors from 2010 to 2013...
March 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29579810/trend-analysis-of-organ-and-tissue-donation-for-transplantation
#10
M J Dos Santos, E Leal de Moraes, M Santini Martins, E Carlos de Almeida, L Borges de Barros E Silva, V Urias, M C Silvano Corrêa Pacheco Furtado, Á Brito Nunes, S El Hage
BACKGROUND: The goal of this study was to identify the tendency toward donations of tissue and organs from donors with brain death between 2001 and 2016 as registered by an organ procurement organization in São Paulo City. METHODS: This quantitative, retrospective, exploratory study encompassed all Tissue and Organ Donation Terms signed between 2001 and 2016. A logistic regression model was applied to verify whether there was an upward or downward trend in donation...
March 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29573222/concise-review-altered-versus-unaltered-amniotic-membrane-as-a-substrate-for-limbal-epithelial-cells
#11
REVIEW
Tor Paaske Utheim, Øygunn Aass Utheim, Panagiotis Salvanos, Catherine J Jackson, Stefan Schrader, Gerd Geerling, Amer Sehic
Limbal stem cell deficiency (LSCD) can result from a variety of corneal disorders, including chemical and thermal burns, infections, and autoimmune diseases. The symptoms of LSCD may include irritation, epiphora, blepharospasms, photophobia, pain, and decreased vision. There are a number of treatment options, ranging from nonsurgical treatments for mild LSCD to various forms of surgery that involve different cell types cultured on various substrates. Ex vivo expansion of limbal epithelial cells (LEC) involves the culture of LEC harvested either from the patient, a living relative, or a cadaver on a substrate in the laboratory...
March 23, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29568941/bradykinin-potentially-stimulates-cell-proliferation-in-rabbit-corneal-endothelial-cells-through-the-zo%C3%A2-1-zonab-pathway
#12
Lixian He, Zhou Zhou, Yi Shao, Zhen Yang, Shuangshuang Zhou, Xuexiang Zou, Ying Zhou, Gang Tan
Bradykinin (BK) has been demonstrated to induce proliferation in several types of cell in ex vivo corneas. However, the mechanisms underlying the action of BK on corneal endothelial cells (CECs) remain largely unknown. The present study aimed to investigate the effect of BK on rabbit corneal endothelial cell (RCEC) proliferation, and assess the involvement of the zonula occludens‑1(ZO‑1)/ZO‑1associated nucleic acid binding protein (ZONAB) pathway. Cell proliferation and cell cycle distribution was analyzed following treatment with BK (0...
March 22, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29567793/corneal-transplant-follow-up-study-ii-ctfs-ii-a-prospective-clinical-trial-to-determine-the-influence-of-hla-class-ii-matching-on-corneal-transplant-rejection-baseline-donor-and-recipient-characteristics
#13
W John Armitage, Helen L Winton, Mark N A Jones, Julie M Crewe, Chris A Rogers, Derek M Tole, Andrew D Dick
PURPOSE: To describe a study to determine the influence of HLA class II matching on allograft rejection of high-risk, full-thickness corneal transplants. METHODS: A prospective, longitudinal, clinical trial (ISRCTN25094892) with a primary outcome measure of time to first clinically determined rejection episode. Tissue typing used DNA-based techniques. Corneas were allocated to patients with ≤2 human leucocyte antigen (HLA) class I antigen mismatches by cohort minimisation to achieve 0, 1 or 2 HLA class II (HLA-DR) antigen mismatches...
March 22, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29555247/post-keratoplasty-endophthalmitis-by-multidrug-resistant-pseudomonas-aeruginosa-with-positive-culture-of-the-contralateral-donor-cornea-a-case-report
#14
J C Serna-Ojeda, L Pedro-Aguilar, C Rodriguez-Quintanilla, H Mejía-López, D G Ponce-Angulo, A Navas, V M Bautista-de Lucio, E O Graue-Hernandez
PURPOSE: The aim of this paper is to present the case report of a patient developing endophthalmitis after penetrating keratoplasty caused by a multidrug-resistant Pseudomonas aeruginosa, detected only in the contralateral donor tissue. CASE REPORT: A 77-year-old man underwent an uneventful penetrating keratoplasty with a preoperative culture-negative donor cornea; however, the fellow cornea grew multidrug-resistant Pseudomonas aeruginosa. The patient developed and was treated for endophthalmitis after penetrating keratoplasty, and aqueous and vitreous taps grew P...
March 16, 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29553420/university-students-knowledge-of-corneal-donation-and-willingness-to-donate-corneas-in-the-occupied-palestinian-territory-a-cross-sectional-study
#15
Liana Al-Labadi, Yazan Gammoh, Reham Shehada, Reem Shahin, Walaa Jbarah, Madleen Amro, Hanan Athamny
BACKGROUND: Access to corneal transplantation is limited worldwide because of poor knowledge. Ethical, religious, and cultural barriers contribute to low rates of corneal donation. In the occupied Palestinian territory, limited information is available on factors affecting corneal donation. The aim of this study was to assess the knowledge and willingness towards corneal donation in Palestinian students. METHODS: This cross-sectional study included university students selected through convenience sampling in Nablus in the summer of 2016...
February 21, 2018: Lancet
https://www.readbyqxmd.com/read/29536619/postnatal-periodontal-ligament-as-a-novel-adult-stem-cell-source-for-regenerative-corneal-cell-therapy
#16
Gary Hin-Fai Yam, Ericia Pei-Wen Teo, Melina Setiawan, Matthew J Lovatt, Nur Zahirah Binte M Yusoff, Matthias Fuest, Bee-Tin Goh, Jodhbir S Mehta
Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest...
March 13, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29532443/htlv-1-myelopathy-after-renal-transplant-and-antiviral-prophylaxis-the-need-for-screening
#17
David Moreno-Ajona, José Ramón Yuste, Paloma Martín, Jaime Gállego Pérez-Larraya
The human T-lymphotropic virus type 1 (HTLV-1) is a RNA retrovirus that infects a minimum of 5-10 million people worldwide. Transmission by cell-containing blood products and solid organ transplantation has been reported. Clinical disease occurs in about 5-10% of infected individuals and consists mainly in adult T cell leukemia and HTLV-1-associated myelopathy (HAM). We present a 54-year-old woman who underwent kidney transplant from cadaveric donor in March 2015. Donor also underwent cornea extraction for another recipient (corneal transplant protocol includes HTLV-1/2 serology)...
March 12, 2018: Journal of Neurovirology
https://www.readbyqxmd.com/read/29531856/human-corneal-endothelial-cell-transplantation-using-nanocomposite-gel-sheet-in-bullous-keratopathy
#18
Periasamy Parikumar, Kazutoshi Haraguchi, Rajappa Senthilkumar, Samuel Jk Abraham
Transplantation of in vitro expanded human corneal endothelial precursors (HCEP) cells using a nanocomposite (D25-NC) gel sheet as supporting material in bovine's cornea has been earlier reported. Herein we report the transplantation of HCEP cells derived from a cadaver donor cornea to three patients using the NC gel sheet. In three patients with bullous keratopathy, one after cataract surgery, one after trauma and another in the corneal graft, earlier performed for congenital corneal dystrophy, not amenable to medical management HCEP cells isolated from a human cadaver donor cornea in vitro expanded using a thermoreversible gelation polymer (TGP) for 26 days were divided into three equal portions and 1...
2018: American Journal of Stem Cells
https://www.readbyqxmd.com/read/29529022/proteomic-analysis-of-corneal-endothelial-cell-descemet-membrane-tissues-reveals-influence-of-insulin-dependence-and-disease-severity-in-type-2-diabetes-mellitus
#19
Jessica M Skeie, Benjamin T Aldrich, Andrew S Goldstein, Gregory A Schmidt, Cynthia R Reed, Mark A Greiner
The objective of this study was to characterize the proteome of the corneal endothelial cell layer and its basement membrane (Descemet membrane) in humans with various severities of type II diabetes mellitus compared to controls, and identify differentially expressed proteins across a range of diabetic disease severities that may influence corneal endothelial cell health. Endothelium-Descemet membrane complex tissues were peeled from transplant suitable donor corneas. Protein fractions were isolated from each sample and subjected to multidimensional liquid chromatography and tandem mass spectrometry...
2018: PloS One
https://www.readbyqxmd.com/read/29528002/descemet-membrane-endothelial-keratoplasty-outcomes-in-the-first-year-of-experience
#20
Dilek D Altınörs, Leyla Asena
OBJECTIVES: We aimed to report the clinical outcomes of Descemet membrane endothelial keratoplasty in our first year of experience. MATERIALS AND METHODS: Patients who underwent Descemet membrane endothelial keratoplasty at the Baskent University Faculty of Medicine, Department of Ophthalmology, between 2015 and 2016 were included in the study. Patient demographics, cause of endothelial dysfunction, best-corrected visual acuity, central corneal thickness, graft survival, follow-up duration, and intraoperative and postoperative complications were recorded...
March 2018: Experimental and Clinical Transplantation
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