keyword
MENU ▼
Read by QxMD icon Read
search

cornea transplantation

keyword
https://www.readbyqxmd.com/read/29208552/controlling-human-corneal-stromal-stem-cell-contraction-to-mediate-rapid-cell-and-matrix-organization-of-real-architecture-for-3-dimensional-tissue-equivalents
#1
Dev Mukhey, James Phillips, Julie T Daniels, Alvena K Kureshi
The architecture of the human corneal stroma consists of a highly organized extracellular matrix (ECM) interspersed with keratocytes. Their progenitor cells; corneal stromal stem cells (CSSC) are located at the periphery, in the limbal stroma. A highly organized corneal ECM is critical for effective transmission of light but this structure may be compromised during injury or disease, resulting in loss of vision. Re-creating normal organization in engineered tissue equivalents for transplantation often involves lengthy culture times that are inappropriate for clinical use or utilisation of synthetic substrates that bring complications such as corneal melting...
December 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29198664/relatives-perception-after-tissue-donation-experience-results-of-a-postdonation-phone-survey
#2
R Roque, C Rodríguez-Villar, A Ruíz, D Paredes, R Gelpi, S Rodríguez, F J García, R Adalia
INTRODUCTION: Postmortem tissue donation (TD) requires the establishment of strategies for family approach to clearly explain the characteristics of multi-tissue donation. In a tertiary university hospital with a long tradition of tissue generation, we designed a survey to be applied to tissue donor families to evaluate global hospital care, care from Transplant Coordinators (TC), quality and content of information given about TD, experience, and motivations after TD process. METHODOLOGY: A prospective phone survey of 10 multiple-choice items was conducted to all TD relatives that agreed to donate one or more tissues...
December 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29193851/survival-of-corneal-nerve-sheath-structures-in-organ-cultured-donor-corneas
#3
Virinder K Dhillon, Mohamed S Elalfy, Marco Messina, Mouhamed Al-Aqaba, Harminder S Dua
PURPOSE: To study the morphology of human corneal nerves in eye bank organ-cultured corneas and in corneal grafts post-transplantation. METHODS: Thirty-seven organ-cultured corneas were divided into: Group-A, anterior 300-400 μm of 20 corneas used for Descemets stripping endothelial keratoplasty, and Group-B, 17 full-thickness corneas unsuitable for transplantation. Corneas whole mounts were stained for nerves using acetylcholinesterase technique and examined by NanoZoomer digital pathology microscope...
November 29, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/29170974/comparative-proteomic-analysis-of-amnion-membrane-transplantation-and-cross-linking-treatments-in-an-experimental-alkali-injury-model
#4
Sevgi Subasi, Ozgul Altintas, Murat Kasap, Nil Guzel, Gurler Akpinar, Suleyman Karaman
PURPOSE: In this study, by using a two-dimensional (2D) electrophoresis-based experimental approach, we aimed at understanding the nature of alkali injuries and the underlying mechanisms. A secondary aim was to compare the effects of cross-linking (CXL) and amnion membrane transplantation (AMT) on corneal protein compositions at the end of the early repair phase after injured with alkali. METHOD: The right corneas of 24 rabbits were injured with a 1 N solution of NaOH...
November 23, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29165417/-pterygium-etiology-pathogenesis-treatment
#5
S A Malozhen, S V Trufanov, D A Krakhmaleva
Pterygium is a degenerative condition characterized by fibrovascular outgrowth of conjunctiva over the cornea. Many theories exist that try to explain its pathogenesis. The current belief is that this disease is multifactorial with ultraviolet radiation being the most important trigger. Attention is also paid to such factors as tear film changes, cytokines and growth factors disbalance, immunologic disturbances, genetic mutations, and viral infections. Modern classifications consider the rate of fibrovascular growth, its progressive potential, and histological features...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/29149984/corneal-xenotransplantation-from-pig-to-rhesus-monkey-no-signs-of-transmission-of-endogenous-porcine-retroviruses
#6
A Li, Y Zhang, Y Liu, Z Pan
BACKGROUND: Xenotransplantations of pig corneas have become an attractive alternative to human corneas. Such xenotransplantations carry the danger, however, of transmission of porcine endogenous retroviruses (PERVs). Here, we investigated whether porcine corneas harbor viral DNA and RNA and whether transplantation to a nonhuman primate would lead to host PERV infection. METHODS: Monkey vein endothelial cells (MVECs) were inoculated with porcine aortic endothelial cell (PAEC) supernatants, and DNA and total RNA of MVECs were tested for PERV by polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR) assays...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29149876/impact-of-donor-graft-quality-on-deep-anterior-lamellar-keratoplasty-dalk
#7
Friederike Schaub, Philip Enders, Werner Adler, Björn O Bachmann, Claus Cursiefen, Ludwig M Heindl
BACKGROUND: To evaluate main features of donor tissue that may influence clinical outcome or complication rate after deep anterior lamellar keratoplasty (DALK). METHODS: Donor tissue parameters of 84 consecutive corneal donor grafts used for big-bubble DALK surgery between June 2011 and December 2014 in 84 eyes of 84 patients with disorders of anterior corneal stroma were correlated to clinical outcome parameters of recipient eyes 12 months after surgery and 3 months after total suture removal...
November 17, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29149239/label-free-in-vivo-imaging-of-corneal-lymphatic-vessels-using-microscopic-optical-coherence-tomography
#8
Jens Horstmann, Hinnerk Schulz-Hildebrandt, Felix Bock, Sebastian Siebelmann, Eva Lankenau, Gereon Hüttmann, Philipp Steven, Claus Cursiefen
Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). Methods: Corneal neovascularization was induced by intrastromal placement of 11...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29146208/glaucoma-associated-corneal-endothelial-cell-damage-a-review
#9
REVIEW
Ben J Janson, Wallace L Alward, Young H Kwon, Daniel I Bettis, John H Fingert, Lorraine M Provencher, Kenneth M Goins, Michael D Wagoner, Mark A Greiner
The corneal endothelium is critical in maintaining a healthy and clear cornea. Corneal endothelial cells have a significant reserve function, but preservation of these cells is paramount as they have limited regenerative capacity. Glaucoma is a prevalent disease, and damage to the corneal endothelium may be caused by the disease process itself as well as by its treatment. The mechanisms involved in glaucoma-associated damage to the corneal endothelium need further investigation. Understanding how glaucoma and glaucoma surgery impact the endothelium is important for protecting corneal clarity and visual acuity in all glaucoma patients, including those undergoing corneal transplant...
November 13, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/29121878/comparison-of-femtosecond-laser-assisted-corneal-intrastromal-xenotransplantation-and-the-allotransplantation-in-rhesus-monkeys
#10
He Jin, Liangping Liu, Hui Ding, Miao He, Chi Zhang, Xingwu Zhong
BACKGROUND: In our previous study, we showed that both allogeneic and autogeneic small-incision femtosecond laser-assisted corneal intrastromal transplantation are safe and effective surgeries. However, the results of small-incision femtosecond laser-assisted intrastromal xenotransplantation have not yet been explored. Additionally, we suggest that glycerol-dehydrated corneal lamellae might provide a possible alternative for this xenogenic implantation approach. METHODS: Corneal inlay lamellae were produced from rabbits and humans using femtosecond laser-assisted surgeries and were dehydrated in glycerol for 1 week at 4 °C...
November 9, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29118495/emergency-visits-after-corneal-transplantation-in-yemen
#11
Mahfouth Abdalla Bamashmus, Saleh A Al-Akily, Hisham A AlAkhalee, Khaldoon O Al-Nuseriy, Marwan H Farhan
PURPOSE: Awareness of symptoms and signs of possible complications after corneal transplantation is very important. Early presentation can enhance long-term survival of the cornea. This study evaluates the reasons for emergency presentation and management of postcorneal transplantation complications. PATIENTS AND METHODS: This retrospective study included a total of 134 postkeratoplasty patients at the cornea unit in Yemen Magrabi Eye Hospital in Sana'a between 2008 and 2010...
September 2017: Oman Journal of Ophthalmology
https://www.readbyqxmd.com/read/29116027/poly-ethylene-glycol-modified-silk-fibroin-membrane-as-a-carrier-for-limbal-epithelial-stem-cell-transplantation-in-a-rabbit-lscd-model
#12
Yijian Li, Yuli Yang, Lei Yang, Yuxiao Zeng, Xiaowei Gao, Haiwei Xu
BACKGROUND: Limbal epithelial stem cells (LESCs) play important roles in corneal epithelial homeostasis and regeneration, and damage to the limbus will lead to limbal stem cell deficiency (LSCD), with conjunctivalization and even visual impairment. Cultured LESCs have been used for ocular surface reconstruction, and silk fibroin (SF) membranes have shown potential as a substrate for LESC cultivation. Both culture methods and the carriers of LESCs affect outcomes following LESC transplantation...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29109898/limbal-stem-cell-deficiency-and-treatment-with-stem-cell-transplantation
#13
REVIEW
Özlem Barut Selver, Ayşe Yağcı, Sait Eğrilmez, Mehmet Gürdal, Melis Palamar, Türker Çavuşoğlu, Utku Ateş, Ali Veral, Çağrı Güven, Jose Mario Wolosin
The cornea is the outermost tissue of the eye and it must be transparent for the maintenance of good visual function. The superficial epithelium of the cornea, which is renewed continuously by corneal stem cells, plays a critical role in the permanence of this transparency. These stem cells are localized at the cornea-conjunctival transition zone, referred to as the limbus. When this zone is affected/destroyed, limbal stem cell deficiency ensues. Loss of limbal stem cell function allows colonization of the corneal surface by conjunctival epithelium...
October 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29107055/native-and-synthetic-scaffolds-for-limbal-epithelial-stem-cell-transplantation
#14
REVIEW
Kim N Nguyen, Samantha Bobba, Alexander Richardson, Mijeong Park, Stephanie L Watson, Denis Wakefield, Nick Di Girolamo
Limbal stem cell deficiency (LSCD) is a complex blinding disease of the cornea, which cannot be treated with conventional corneal transplants. Instead, a stem cell (SC) graft is required to replenish the limbal epithelial stem cell (LESC) reservoir, which is ultimately responsible for regenerating the corneal epithelium. Current therapies utilize limbal tissue biopsies that harbor LESCs as well as tissue culture expanded cells. Typically, this tissue is placed on a scaffold that supports the formation of corneal epithelial cell sheets, which are then transferred to diseased eyes...
November 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29105366/limbal-stem-cells-identity-developmental-origin-and-therapeutic-potential
#15
REVIEW
Gabriel Gonzalez, Yuzuru Sasamoto, Bruce R Ksander, Markus H Frank, Natasha Y Frank
The cornea is our window to the world and our vision is critically dependent on corneal clarity and integrity. Its epithelium represents one of the most rapidly regenerating mammalian tissues, undergoing full-turnover over the course of approximately 1-2 weeks. This robust and efficient regenerative capacity is dependent on the function of stem cells residing in the limbus, a structure marking the border between the cornea and the conjunctiva. Limbal stem cells (LSC) represent a quiescent cell population with proliferative capacity residing in the basal epithelial layer of the limbus within a cellular niche...
November 3, 2017: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29104070/the-role-of-amniotic-membrane-extract-eye-drop-ameed-in-in-vivo-cultivation-of-limbal-stem-cells
#16
Alireza Baradaran-Rafii, Niloufar Shayan Asl, Marzieh Ebrahimi, Sayena Jabbehdari, Shahram Bamdad, Danial Roshandel, Medi Eslani, Maryam Momeni
BACKGROUND: Limbal stem cell transplantation (LSCT) is the definitive treatment for total limbal stem cell deficiency (LSCD). This study evaluates the anatomical and visual outcomes of a surgical technique supplemented by amniotic membrane extract eye drop (AMEED) for in vivo cultivation of limbal stem cells (LSCs). METHODS: One small limbal block (2 × 1 mm) harvested from the contralateral healthy eye was transferred to the diseased eye, which had been already covered by cryopreserved amniotic membrane (N = 20)...
November 2, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29103962/corneal-stroma-enhancement-with-decellularized-stromal-laminas-with-or-without-stem-cell-recellularization-for-advanced-keratoconus
#17
Jorge L Alió Del Barrio, Mona El Zarif, Albert Azaar, Nehman Makdissy, Charbel Khalil, Walid Harb, Ibrahim El Achkar, Ziad Abdul Jawad, María P de Miguel, Jorge L Alió
PURPOSE: Phase-1 study to preliminarily evaluate the safety and efficacy of decellularized human corneal stromal lamina transplantation with or without autologous adipose derived adult stem cells (ADASC) recellularization within the corneal stroma of patients with advanced keratoconus. DESIGN: Phase one clinical trial METHODS: Femtosecond-assisted 120μm-thickness and 9mm-diameter laminas were obtained from the anterior stroma of human donor corneas and decellularized with a sodium dodecyl sulfate solution...
November 2, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29097211/infectious-crystalline-keratopathy
#18
REVIEW
Ashley J Porter, Graham A Lee, Albert S Jun
Infectious crystalline keratopathy was first reported by Gorovoy and colleagues in 1983 when they identified bacteria colonizing a cornea after a penetrating keratoplasty. Subsequent cases have elaborated on the organisms responsible and the management outcomes. Patients present with a white or gray branching opacity originating from an epithelial defect, commonly after a penetrating keratoplasty. Local immunosuppression contributes to the quiescent nature and the limited inflammatory response associated with infectious crystalline keratopathy...
October 31, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/29090046/preparation-and-thickness-profile-of-endothelial-keratoplasty-lenticules-from-donated-whole-eyes-with-previous-photorefractive-keratectomy
#19
Mozhgan Rezaei Kanavi, Pejman Fahim, Mohsen Rahmanian, Tahereh Chamani, Bahar Kheiri, Sahar Balagholi, Mohammad Ali Javadi
PURPOSE: To describe the preparation and thickness profiles of endothelial keratoplasty lenticules harvested from eyes with previous photorefractive keratectomy (PRK). METHODS: Donor whole eyes that underwent PRK were subjected to microkeratome-assisted dissection for Descemet stripping automated endothelial keratoplasty. Specular microscopy and Visante optical coherence tomography were performed on precut corneas. Endothelial cell indices and thickness profiles of endothelial keratoplasty lenticules were statistically analyzed...
October 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/29079991/establishment-of-a-porcine-corneal-endothelial-organ-culture-model-for-research-purposes
#20
Berenike C Kunzmann, Olaf J C Hellwinkel, Christian Klameth, Daniel Wenzel, Karl U Bartz-Schmidt, Martin S Spitzer, Maximilian Schultheiss
Human corneas usually are not available for research, as they are used for transplantation only. At the same time, scientific studies on cultured human endothelial cells can produce misleading results due to inevitable dedifferentiation. Therefore, an organ-culture model of porcine corneas-displaying endothelial cell death rates comparable to those of cultured human corneas-would be very desirable. Fresh pig eyes were prepared under sterile conditions to obtain corneoscleral buttons, corneal buttons and so called "split corneal buttons" (new preparation method) and cultivated for 15 days...
October 27, 2017: Cell and Tissue Banking
keyword
keyword
79366
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"