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https://www.readbyqxmd.com/read/28707269/complete-genomic-sequence-of-common-reed-chlorotic-stripe-virus-a-novel-member-of-the-family-potyviridae
#1
Wen Yuan, Kaitong Du, Zaifeng Fan, Tao Zhou
Common reed (Phragmites australis) plants showing chlorotic stripe symptoms on leaves were found in Gansu Province, China. Deep sequencing of small RNAs from symptomatic leaves identified a putative potyvirus, which was named common reed chlorotic stripe virus (CRCSV). The full genome sequence was determined by reverse transcription PCR, rapid amplification of cDNA ends (RACE) PCR, and sequencing. It consists of 9,426 nucleotides, excluding the poly(A) tail, and contains a large open reading frame encoding a polyprotein of 3,014 amino acids...
July 13, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28705149/f-treegc-a-questionnaire-based-family-tree-creation-software-for-genetic-counseling-and-genome-cohort-studies
#2
RANDOMIZED CONTROLLED TRIAL
Tomoharu Tokutomi, Akimune Fukushima, Kayono Yamamoto, Yasushi Bansho, Tsuyoshi Hachiya, Atsushi Shimizu
BACKGROUND: The Tohoku Medical Megabank project aims to create a next-generation personalized healthcare system by conducting large-scale genome-cohort studies involving three generations of local residents in the areas affected by the Great East Japan Earthquake. We collected medical and genomic information for developing a biobank to be used for this healthcare system. We designed a questionnaire-based pedigree-creation software program named "f-treeGC," which enables even less experienced medical practitioners to accurately and rapidly collect family health history and create pedigree charts...
July 14, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/28660656/early-identification-of-reading-difficulties-a-screening-strategy-that-adjusts-the-sensitivity-to-the-level-of-prediction-accuracy
#3
Mads Poulsen, Anne-Mette Veber Nielsen, Holger Juul, Carsten Elbro
Early screening for reading difficulties before the onset of instruction is desirable because it allows intervention that is targeted at prevention rather than remediation of reading difficulties. However, early screening may be too inaccurate to effectively allocate resources to those who need them. The present study compared the accuracy of early screening before the onset of formal reading instruction with late screening six months into the first year of instruction. The study followed 164 Danish students from the end of Grade 0 to the end of Grade 2...
June 28, 2017: Dyslexia: the Journal of the British Dyslexia Association
https://www.readbyqxmd.com/read/28651533/profiling-of-antibiotic-resistance-of-bacterial-species-recovered-from-routine-clinical-isolates-in-ethiopia
#4
Robert-Jan Ten Hove, Melaku Tesfaye, Witold Frederik Ten Hove, Mesfin Nigussie
BACKGROUND: With the alarming rise in antibiotic resistance in African countries, the need for a surveillance system in the region has become pressing. The rapid expansion of data networks makes it possible to set up healthcare applications that can be both cost-efficient and effective. Large data sets are available for assessment of current antibiotic resistance among Ethiopian patients. Based on the data-presentation, a practical approach is proposed on how diagnostic laboratories can participate remedial action against antibiotic resistance in Ethiopia...
June 26, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28621157/predicting-individual-differences-in-reading-and-spelling-skill-with-artificial-script-based-letter-speech-sound-training
#5
Sebastián Aravena, Jurgen Tijms, Patrick Snellings, Maurits W van der Molen
In this study, we examined the learning of letter-speech sound correspondences within an artificial script and performed an experimental analysis of letter-speech sound learning among dyslexic and normal readers vis-à-vis phonological awareness, rapid automatized naming, reading, and spelling. Participants were provided with 20 min of training aimed at learning eight new basic letter-speech sound correspondences, followed by a short assessment of mastery of the correspondences and word-reading ability in this unfamiliar script...
June 1, 2017: Journal of Learning Disabilities
https://www.readbyqxmd.com/read/28594387/3d-printing-based-integrated-water-quality-sensing-system
#6
Muinul Banna, Kaustav Bera, Ryan Sochol, Liwei Lin, Homayoun Najjaran, Rehan Sadiq, Mina Hoorfar
The online and accurate monitoring of drinking water supply networks is critically in demand to rapidly detect the accidental or deliberate contamination of drinking water. At present, miniaturized water quality monitoring sensors developed in the laboratories are usually tested under ambient pressure and steady-state flow conditions; however, in Water Distribution Systems (WDS), both the pressure and the flowrate fluctuate. In this paper, an interface is designed and fabricated using additive manufacturing or 3D printing technology-material extrusion (Trade Name: fused deposition modeling, FDM) and material jetting-to provide a conduit for miniaturized sensors for continuous online water quality monitoring...
June 8, 2017: Sensors
https://www.readbyqxmd.com/read/28572891/factors-positively-influencing-health-are-associated-with-a-lower-risk-of-development-of-metabolic-syndrome-in-korean-men-the-2007-2009-korean-national-health-and-nutrition-examination-survey
#7
Shinhye Kim, Mi-Ra Cho, Taejong Kim, Hyoung-Ji Lim, Jae Woo Lee, Hee-Taik Kang
BACKGROUND: The prevalence of metabolic syndrome (MetS) has risen rapidly worldwide, including in South Korea. Factors related to lifestyle are closely associated with the development of MetS. The aim of this study was to investigate the association between MetS and a number of factors positively influencing health, namely non-smoking, low-risk drinking, sufficient sleep, regular exercise, and the habit of reading food labels, among Korean men. METHODS: This cross-sectional study included 3,869 men from the 2007-2009 Korean National Health and Nutrition Examination Survey...
May 2017: Korean Journal of Family Medicine
https://www.readbyqxmd.com/read/28501822/structural-mri-correlates-of-amyotrophic-lateral-sclerosis-progression
#8
Joe Senda, Naoki Atsuta, Hirohisa Watanabe, Epifanio Bagarinao, Kazunori Imai, Daichi Yokoi, Yuichi Riku, Michihito Masuda, Ryoichi Nakamura, Hazuki Watanabe, Mizuki Ito, Masahisa Katsuno, Shinji Naganawa, Gen Sobue
PURPOSE: Amyotrophic lateral sclerosis (ALS) presents with varying degrees of brain degeneration that can extend beyond the corticospinal tract (CST). Furthermore, the clinical course and progression of ALS varies widely. Brain degeneration detected using structural MRI could reflect disease progression. SUBJECTS AND METHODS: On study registration, 3-Tesla volumetric MRI and diffusion tensor imaging scans were obtained at baseline in 38 healthy controls and 67 patients with sporadic ALS...
May 13, 2017: Journal of Neurology, Neurosurgery, and Psychiatry
https://www.readbyqxmd.com/read/28432867/examining-the-direct-and-indirect-effects-of-visual-verbal-paired-associate-learning-on-chinese-word-reading
#9
George Georgiou, Cuina Liu, Shiyang Xu
Associative learning, traditionally measured with paired associate learning (PAL) tasks, has been found to predict reading ability in several languages. However, it remains unclear whether it also predicts word reading in Chinese, which is known for its ambiguous print-sound correspondences, and whether its effects are direct or indirect through the effects of other reading-related skills such as phonological awareness and rapid naming. Thus, the purpose of this study was to examine the direct and indirect effects of visual-verbal PAL on word reading in an unselected sample of Chinese children followed from the second to the third kindergarten year...
August 2017: Journal of Experimental Child Psychology
https://www.readbyqxmd.com/read/28428624/attentional-selection-predicts-rapid-automatized-naming-ability-in-chinese-speaking-children-with-adhd
#10
Encong Wang, Meirong Sun, Ye Tao, Xiaoyi Gao, Jialiang Guo, Chenguang Zhao, Hui Li, Qiujin Qian, Zhanliang Wu, Yufeng Wang, Li Sun, Yan Song
Children with attention-deficit/hyperactivity disorder (ADHD) are reported to have a significantly higher risk of showing reading difficulties or disorders. Here, we aimed to identify the relationship between electroencephalographic (EEG) marker of spatial attention and reading ability in Chinese children with ADHD. First, we demonstrated that rapid automatized naming (RAN) is a strong predictor of reading ability in Chinese-speaking children. Then, EEG data of 9-to 15-year-old children with ADHD (n = 38) and typically developing (TD) controls (n = 36) were collected while the children performed a classical visual search task...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28403224/hstlbo-a-hybrid-algorithm-based-on-harmony-search-and-teaching-learning-based-optimization-for-complex-high-dimensional-optimization-problems
#11
Shouheng Tuo, Longquan Yong, Fang'an Deng, Yanhai Li, Yong Lin, Qiuju Lu
Harmony Search (HS) and Teaching-Learning-Based Optimization (TLBO) as new swarm intelligent optimization algorithms have received much attention in recent years. Both of them have shown outstanding performance for solving NP-Hard optimization problems. However, they also suffer dramatic performance degradation for some complex high-dimensional optimization problems. Through a lot of experiments, we find that the HS and TLBO have strong complementarity each other. The HS has strong global exploration power but low convergence speed...
2017: PloS One
https://www.readbyqxmd.com/read/28362193/relations-between-awareness-of-morphosyntactic-structures-in-chinese-compound-words-and-reading-abilities-a-short-report
#12
Dustin Kai-Yan Lau, Yuan Liang, Man Tak Leung
This is a short report of the investigation of the relationship between awareness of morphosyntactic structures in Chinese compound words and reading abilities on 268 fourth graders studying in three mainstream schools in Shenzhen. All children were assessed using reading and cognitive tasks including rapid automatized naming, phonological, orthographic and morphological awareness. A compound production task using pseudo compound words with different morphosyntactic structures in the first and second levels as stimuli was also conducted...
March 31, 2017: Clinical Linguistics & Phonetics
https://www.readbyqxmd.com/read/28320326/lw-fqzip-2-a-parallelized-reference-based-compression-of-fastq-files
#13
Zhi-An Huang, Zhenkun Wen, Qingjin Deng, Ying Chu, Yiwen Sun, Zexuan Zhu
BACKGROUND: The rapid progress of high-throughput DNA sequencing techniques has dramatically reduced the costs of whole genome sequencing, which leads to revolutionary advances in gene industry. The explosively increasing volume of raw data outpaces the decreasing disk cost and the storage of huge sequencing data has become a bottleneck of downstream analyses. Data compression is considered as a solution to reduce the dependency on storage. Efficient sequencing data compression methods are highly demanded...
March 20, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28299895/deep-sequencing-and-variant-analysis-of-an-italian-pathogenic-field-strain-of-equine-infectious-anaemia-virus
#14
K Cappelli, R F Cook, V Stefanetti, F Passamonti, G L Autorino, M T Scicluna, M Coletti, A Verini Supplizi, S Capomaccio
Equine infectious anaemia virus (EIAV) is a lentivirus with an almost worldwide distribution that causes persistent infections in equids. Technical limitations have restricted genetic analysis of EIAV field isolates predominantly to gag sequences resulting in very little published information concerning the extent of inter-strain variation in pol, env and the three ancillary open reading frames (ORFs). Here, we describe the use of long-range PCR in conjunction with next-generation sequencing (NGS) for rapid molecular characterization of all viral ORFs and known transcription factor binding motifs within the long terminal repeat of two EIAV isolates from the 2006 Italian outbreak...
March 15, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28287980/constructing-dna-barcode-sets-based-on-particle-swarm-optimization
#15
Bin Waang, Xuedong Zheng, Shihua Zhou, Changjun Zhou, Xiaopeng Wei, Qiang Zhang, Ziqi Wei
Following the completion of the human genome project, a large amount of high-throughput bio-data was generated. To analyze these data, massively parallel sequencing, namely next-generation sequencing, was rapidly developed. DNA barcodes are used to identify the ownership between sequences and samples when they are attached at the beginning or end of sequencing reads. Constructing DNA barcode sets provides the candidate DNA barcodes for this application. To increase the accuracy of DNA barcode sets, a particle swarm optimization (PSO) algorithm has been modified and used to construct the DNA barcode sets in this paper...
March 7, 2017: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://www.readbyqxmd.com/read/28279619/led-fluorescence-microscopy-novel-method-for-malaria-diagnosis-compared-with-routine-methods
#16
Riddhi Hathiwala, Preeti R Mehta, Gita Nataraj, Siddhi Hathiwala
Rapid and accurate diagnosis of malaria is the need of hour for effective management and controlling drug resistance. The conventional and gold-standard method, Light microscopy (LM), is time-consuming, requires trained staff and well-maintained equipments. The newly developed, rapid diagnostic tests (RDT) are fast and reliable, but give only qualitative results, are expensive and have short shelf life. Light Emission Diode fluorescence microscopy (LED FM) may provide a reliable alternative which can be used for routine diagnosis...
March 6, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28257277/the-effect-of-in-school-saccadic-training-on-reading-fluency-and-comprehension-in-first-and-second-grade-students
#17
David Dodick, Amaal J Starling, Jennifer Wethe, Yi Pang, Leonard V Messner, Craig Smith, Christina L Master, Rashmi B Halker-Singh, Bert B Vargas, Jamie M Bogle, Jay Mandrekar, Alexandra Talaber, Danielle Leong
Efficient eye movements provide a physical foundation for proficient reading skills. We investigated the effect of in-school saccadic training on reading performance. In this cross-over design, study participants (n = 327, 165 males; mean age [SD]: 7 y 6 mo [1y 1 mo]) were randomized into treatment and control groups, who then underwent eighteen 20-minute training sessions over 5 weeks using King-Devick Reading Acceleration Program Software. Pre- and posttreatment reading assessments included fluency, comprehension, and rapid number naming performance...
January 2017: Journal of Child Neurology
https://www.readbyqxmd.com/read/28208281/enzyme-activated-g-quadruplex-synthesis-for-in-situ-label-free-detection-and-bioimaging-of-cell-apoptosis
#18
Zhuoliang Liu, Xingyu Luo, Zhu Li, Yan Huang, Zhou Nie, Hong-Hui Wang, Shouzhuo Yao
Fluorogenic probes targeting G-quadruplex structures have emerged as the promising toolkit for functional research of G-quadruplex and biosensor development. However, their biosensing applications are still largely limited in in-tube detection. Herein, we proposed a fluorescent bioimaging method based on enzyme-generated G-quadruplexes for detecting apoptotic cells at the cell and tissue level, namely, terminal deoxynucleotidyl transferase (TdT)-activated de novo G-quadruplex synthesis (TAGS) assay. The detection target is genomic DNA fragmentation, a biochemical hallmark of apoptosis...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28191331/development-of-dual-taqman-based-one-step-rrt-pcr-assay-panel-for-rapid-and-accurate-diagnostic-test-of-mers-cov-a-novel-human-coronavirus-ahead-of-hajj-pilgrimage
#19
Mohammad Sadegh Hashemzadeh, Rahimeh Rasouli, Bentolhoda Zahraei, Morteza Izadi, Mahdi Tat, Seyed Hassan Saadat, Mohammad Najarasl, Behzad Khansari Nejad, Ruhollah Dorostkar
BACKGROUND: Coronaviruses (CoVs) are large ribonucleic acid (RNA) viruses causing primarily respiratory disease in humans. A novel human coronavirus, subsequently named middle east respiratory syndrome coronavirus (MERS-CoV), was first reported in Saudi Arabia in September of 2012. With increasing numbers of infections and deaths from MERS-CoV, development of a rapid and reliable kit was crucial to prevent further spread of MERS-CoV. OBJECTIVES: In this study, we present two real-time reverse-transcription polymerase chain reaction (rRT-PCR) assays for in-house rapid and sensitive diagnostic testing of MERS-CoV, detecting the regions upstream of the envelope gene (upE) and open reading frame (ORF) 1b, respectively, for initial screening and final confirmation of MERS-CoV infection, as recommended by the world health organization (WHO)...
November 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/28110086/different-mechanisms-in-learning-different-second-languages-evidence-from-english-speakers-learning-chinese-and-spanish
#20
Fan Cao, Bethany L Sussman, Valeria Rios, Xin Yan, Zhao Wang, Gregory J Spray, Ryan M Mack
Word reading has been found to be associated with different neural networks in different languages, with greater involvement of the lexical pathway for opaque languages and greater invovlement of the sub-lexical pathway for transparent langauges. However, we do not know whether this language divergence can be demonstrated in second langauge learners, how learner's metalinguistic ability would modulate the langauge divergence, or whether learning method would interact with the language divergence. In this study, we attempted to answer these questions by comparing brain activations of Chinese and Spanish word reading in native English-speaking adults who learned Chinese and Spanish over a 2 week period under three learning conditions: phonological, handwriting, and passive viewing...
January 19, 2017: NeuroImage
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