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Dandan Wang, Guoguang Chen, Lili Ren
Amiodarone hydrochloride (AMD) is used in the treatment of a wide range of cardiac tachyarrhythmias, including both ventricular fibrillation (VF) and hemodynamically unstable ventricular tachycardia (VT). The objectives of this study were to improve the solubility and bioavailability in fasted state and to reduce the food effect of AMD by producing its inclusion complex with sulfobutylether-β-cyclodextrin (SBE-β-CD). The complex was prepared through a saturated water solution combined with the freeze-drying method and then characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and differential scanning calorimetry...
October 18, 2016: AAPS PharmSciTech
Longbin Zheng, Ying Li, Xuesong Li, Jiayuan Kou, Zhaoyu Zhong, Yueqing Jiang, Zhongni Liu, Ye Tian, Liming Yang
BACKGROUND/AIMS: Sonodynamic therapy (SDT) is considered a new approach for the treatment of atherosclerosis. We previously confirmed that hydroxyl acetylated curcumin (HAC) was a sonosensitizer. In this study, we investigated the mechanism of THP-1 macrophage apoptosis and autophagy induced by HAC mediated SDT (HAC-SDT). METHODS: Cell viability was measured using a CCK-8 assay. Laser scanning confocal microscopy was used to measure the levels of intracellular reactive oxygen species (ROS), sub-cellular HAC localization, BAX and cytochrome C translocation, LC3 expression, monodansylcadaverine staining and Dil-labeled oxidized low density lipoprotein (Dil-ox-LDL) uptake...
October 17, 2016: Cellular Physiology and Biochemistry
Zhiyong Xie, Juncui Xu, Fazhi Xie, Shiquan Xiong
A reduced graphene oxide (rGO)/Fe3O4 composites modified screen-printed carbon electrode (SPCE) was used to determinate As(III) in a HAc-NaAc buffer solution. The rGO/Fe3O4 composites were prepared by a simple and one-pot synthesis method, and characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (SEM), FT-IR and Raman spectra. The electrochemical behaviors of the composite electrode were characterized by cycle voltammetry and electrochemical impedance spectroscopy. The experimental parameters, such as supporting electrolyte, solution pH, deposition potential, deposition time were optimized, respectively...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Kate Grossman, Mary Beth Beasley, Sidney S Braman
RATIONALE: Hepatoid adenocarcinoma (HAC) is a rare malignant lung tumor that histologically resembles typical hepatocellular carcinoma (HCC) when it is metastatic to the lung. To date, this clinical entity has not been highlighted in the pulmonary literature. OBJECTIVE: We present a review of all known cases of HAC, including the relevant clinical and histopathological features important for pulmonologists. MEASUREMENTS AND MAIN RESULTS: The purpose of this report is to present a new case of HAC, with typical clinical and histologic features of this malignancy, and to summarize findings of previously reported cases...
October 2016: Respiratory Medicine
Jae Hac Ko, Zeyu Ma, Xiao Jin, Qiyong Xu
Research has been conducted to investigate the effects of daily aeration frequency on leachate quality and waste settlement in simulated hybrid landfill bioreactors. Four laboratory-scale reactors were constructed and operated for about 10 months to simulate different bioreactor operations, including one anaerobic bioreactor and three hybrid bioreactors with different aeration frequencies (1, 2, and 4 times per day). Chemical oxygen demand (COD) and biochemical oxygen demand (BOD5) reduced more than 96% of the initial concentrations in all aerated bioreactors...
September 15, 2016: Journal of the Air & Waste Management Association
Roberta Pascolutti, Xianqiang Sun, Joseph Kao, Roy L Maute, Aaron M Ring, Gregory R Bowman, Andrew C Kruse
The immune checkpoint receptor PD-1 and its ligand, PD-L1, have emerged as key regulators of anti-tumor immunity in humans. Recently, we reported an ultra-high-affinity PD-1 mutant, termed high-affinity consensus (HAC) PD-1, which shows superior therapeutic efficacy in mice compared with antibodies. However, the molecular details underlying the action of this agent remain incompletely understood, and a molecular view of PD-1/PD-L1 interactions in general is only beginning to emerge. Here, we report the structure of HAC PD-1 in complex with PD-L1, showing that it binds PD-L1 using a unique set of polar interactions...
October 4, 2016: Structure
Nathalie Mayer, Silvia Lopa, Giuseppe Talò, Arianna B Lovati, Marielle Pasdeloup, Stefania A Riboldi, Matteo Moretti, Frédéric Mallein-Gerin
Articular cartilage has poor healing ability and cartilage injuries often evolve to osteoarthritis. Cell-based strategies aiming to engineer cartilaginous tissue through the combination of biocompatible scaffolds and articular chondrocytes represent an alternative to standard surgical techniques. In this context, perfusion bioreactors have been introduced to enhance cellular access to oxygen and nutrients, hence overcoming the limitations of static culture and improving matrix deposition. Here, we combined an optimized cocktail of soluble factors, the BIT (BMP-2, Insulin, Thyroxin), and clinical-grade collagen sponges with a bidirectional perfusion bioreactor, namely the oscillating perfusion bioreactor (OPB), to engineer in vitro articular cartilage by human articular chondrocytes (HACs) obtained from osteoarthritic patients...
2016: PloS One
Ltk Do, M Wittayarat, T Terazono, Y Sato, M Taniguchi, F Tanihara, T Takemoto, Y Kazuki, K Kazuki, M Oshimura, T Otoi
The current applications for cat cloning include production of models for the study of human and animal diseases. This study was conducted to investigate the optimal fusion protocol on in vitro development of transgenic cloned cat embryos by comparing duration of electric pulse. Cat fibroblast cells containing a human artificial chromosome (HAC) vector were used as genetically modified nuclear donor cells. Couplets were fused and activated simultaneously with a single DC pulse of 3.0 kV/cm for either 30 or 60 μs...
August 28, 2016: Reproduction in Domestic Animals, Zuchthygiene
Ang Ren, Rui Liu, Zhi-Gang Miao, Xue Zhang, Peng-Fei Cao, Tian-Xi Chen, Chen-Yang Li, Liang Shi, Ai-Liang Jiang, Ming-Wen Zhao
Ganoderma lucidum is one of the most important medicinal fungi, but the lack of basic study on the fungus has hindered the further development of its value. To investigate the roles of the redox system in G. lucidum, acetic acid (HAc) was applied as a reactive oxygen species (ROS) stress inducer, and hydrogen-rich water (HRW) was used to relieve the ROS stress in this study. Our results demonstrate that the treatment of 5% HRW significantly decreased the ROS content, maintained biomass and polar growth morphology of mycelium, and decreased secondary metabolism under HAc-induced oxidative stress...
August 23, 2016: Environmental Microbiology
Kaczorowska-Hac Barbara, Luszczyk Marcin, Antosiewicz Jedrzej, Ziolkowski Wieslaw, Adamkiewicz-Drozynska Elzbieta, Mysliwiec Malgorzata, Milosz Ewa, Kaczor Jan Jacek
The molecular mechanism that regulates iron homeostasis is based on a network of signals, which reflect on the iron requirements of the body. Hereditary hemochromatosis is a heterogenic metabolic syndrome which is due to unchecked transfer of iron into the bloodstream and its toxic effects on parenchymatous organs. It is caused by the mutation of genes that encode proteins that help hepcidin to monitor serum iron. These proteins include the human hemochromatosis protein -HFE, transferrin-receptor 2, hemojuvelin in rare instances, and ferroportin...
August 24, 2016: Annals of Hematology
Jessica S Chen, Ron A Adelman
BACKGROUND AND OBJECTIVE: Timely treatment of age-related macular degeneration (AMD) is integral in improving outcomes. To catch choroidal neovascularization as soon as possible, patients should monitor vision at home. The objective of this study is to explore the Hyperacuity App (HAC) as a screen for progression of disease in AMD. PATIENTS AND METHODS: A cross-sectional, single-center study was performed with 33 subjects. Consent was obtained and patient information was protected in accordance with the protocol approved by the Yale Human Research Protection Program...
August 1, 2016: Ophthalmic Surgery, Lasers & Imaging Retina
Kayvan Mohkam, Florian Fanget, Benjamin Darnis, Brahim Harbaoui, Agnès Rode, Bruno Charpiat, Christian Ducerf, Jean-Yves Mabrut
BACKGROUND: Doppler ultrasound arterial abnormalities (DAA) after orthotopic liver transplantation (OLT) often represent a sign of hepatic artery (HA) complication (HAC). The standard management of DAA involves computed tomographic angiography (CTA) followed by invasive vascular intervention (IVI) or observation. We evaluated the contribution of systemic vasodilators (SVD) to the management of DAA after OLT. METHODS: Between 2005 and 2015, 91 out of 514 OLT recipients developed DAA (defined by HA resistive index [HARI] <0...
August 19, 2016: Transplantation
Yong Wang, Liyuan Chai, Zhihui Yang, Mubarak Hussani, Ruiyang Xiao, Chongjian Tang
Ficus tikoua (F. tikoua) was a promising species for Antimony (Sb) phyto-remediation due to widely growing in mining area. However, little was known on its tolerance mechanisms towards Sb. The determination of the distribution and chemical speciation of Sb in F. tikoua is essential for understanding the mechanisms involved in Sb accumulation, transportation, and detoxification. The present study investigated the subcellular distribution and chemical forms of Sb in F. tikoua. The plant was exposed to different Sb concentrations (0, 30, 90 and 180 μmol/L) for 30 days...
August 19, 2016: International Journal of Phytoremediation
Elisa Michel, Clarisse Monfort, Marion Deffrasnes, Stéphane Guezenec, Emilie Lhomme, Matthieu Barret, Delphine Sicard, Xavier Dousset, Bernard Onno
In order to contribute to the description of sourdough LAB composition, MiSeq sequencing and qPCR methods were performed in association with cultural methods. A panel of 16 French organic bakers and farmer-bakers were selected for this work. The lactic acid bacteria (LAB) diversity of their organic sourdoughs was investigated quantitatively and qualitatively combining (i) Lactobacillus sanfranciscensis-specific qPCR, (ii) global sequencing with MiSeq Illumina technology and (iii) molecular isolates identification...
July 29, 2016: International Journal of Food Microbiology
Ling Tong, Huaxiong Pan, Jun He, Mixia Weng, Liduan Zheng
INTRODUCTION: Hepatoid adenocarcinoma (HAC) is a rare neoplasm with a striking morphologic similarity to hepatocellular carcinoma. The most common sites of HAC are the stomach, lung, and pancreas. CASE REPORT: Here we report a rare case of HAC arising from the heterotopic pancreas (Heinrich type II) in the ileum with lymph node metastasis. A 56-year-old man was admitted to our hospital presenting with bloody stools under no obvious predisposing causes. The colonoscopy and the gastroscopy showed no pathological findings...
August 2016: Medicine (Baltimore)
Su Jung Choi, Sung Ik Lee, Eun Yeon Joo
To investigate the associations between amount of habitual alcohol consumption (HAC) and prevalence of metabolic syndrome (MetS), sleep, and sleep-disordered breathing (SDB). We enrolled 683 untreated SDB male patients (age: 54.4 ± 7.80 y, apnea-hypopnea index (AHI): 29.0 ± 21.53/h). HAC was assessed as the average number of drinks consumed per week during the past 12 months. Anthropometric and biochemical markers were used to diagnose MetS. Clinical data and MetS components were compared according to the reported amounts of HAC (no drinking, light drinking <13, heavy drinking ≥13 drinks/week)...
2016: PloS One
Joseph E Tanenbaum, Jacob A Miller, Vincent J Alentado, Daniel Lubelski, Benjamin P Rosenbaum, Edward C Benzel, Thomas E Mroz
BACKGROUND CONTEXT: The incidence of adverse care quality events among patients undergoing cervical fusion surgery is unknown using the definition of care quality employed by the Centers for Medicare and Medicaid Services (CMS). The effect of insurance status on the incidence of these adverse quality events is also unknown. PURPOSE: This study determined the incidence of hospital acquired conditions (HAC) and patient safety indicators (PSI) in cervical spine fusion patients and analyzed the association between primary payer status and these adverse events...
August 4, 2016: Spine Journal: Official Journal of the North American Spine Society
John Di Capua, Sulaiman Somani, Jun S Kim, Dante M Leven, Nathan J Lee, Parth Kothari, Samuel K Cho
STUDY DESIGN: Retrospective study of prospectively collected data. OBJECTIVE: To identify risk factors in developing hospital acquired conditions (HACs) and association of HACs with other 30-day complications in the adult spinal deformity (ASD) population. SUMMARY OF BACKGROUND DATA: HACs are subject to a non-payment policy by the Center for Medicare and Medicaid Services and provide an incentive for medical institutions to improve patient safety...
August 5, 2016: Spine
Katarzyna Hąc-Wydro, Karolina Szydło
Eucalyptus oil is known from antimicrobial properties and due to natural origin and low toxicity it can be applied as a green pesticide. This plant extract is a mixture of compounds among which Eucalyptol prevails strongly over the number of minor components including terpinen-4-ol. However, antimicrobial effect of total extract is stronger than the effect of its major component. The mechanism of action of essential oils involves their incorporation into membrane of pathogenic cell. To investigate the effect of eucalyptus oil components on bacterial membrane in this work the influence of Eucalyptol on model lipid systems of various compositions was studied and then compared with the effect induced by the mixture of Eucalyptol and terpinen-4-ol...
October 1, 2016: Colloids and Surfaces. B, Biointerfaces
Karolin Weitkunat, Sara Schumann, Daniela Nickel, Katharina Antonia Kappo, Klaus Jürgen Petzke, Anna Patricia Kipp, Michael Blaut, Susanne Klaus
SCOPE: The short-chain fatty acids (SCFA) acetate (Ac) and propionate (Pr) are major fermentation products of dietary fibers and provide additional energy to the host. We investigated short and long term effects of dietary Ac and Pr supplementation on diet-induced obesity and hepatic lipid metabolism. METHODS AND RESULTS: C3H/HeOuJ-mice received high-fat (HF) diets supplemented with 5% SCFA in different Ac:Pr ratios, a high acetate (HF-HAc; 2.5:1 Ac:Pr) or high propionate ratio (HF-HPr; 1:2...
July 28, 2016: Molecular Nutrition & Food Research
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