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https://www.readbyqxmd.com/read/28438562/first-successful-vitrification-of-salmonid-ovarian-tissue
#1
Jelena Lujić, Zoran Marinović, Simona Sušnik Bajec, Ida Djurdjevič, Eszter Kása, Béla Urbányi, Ákos Horváth
Due to a lack of cryopreservation protocols for fish eggs and embryos, alternative techniques which will enable storage of female genetic resources are crucial for future development of reproduction management in conservation biology and aquaculture. Experiments were conducted to develop an optimal vitrification protocol for cryopreservation of brown trout Salmo trutta juvenile ovarian tissue. Needle immersed vitrification (NIV) method was used where ovaries were pinned on an acupuncture needle, passaged through equilibration and vitrification solutions containing different combinations and concentrations of methanol (MeOH), propylene glycol (PG) and dimethyl sulfoxide (Me2SO) and subsequently plunged into liquid nitrogen...
April 21, 2017: Cryobiology
https://www.readbyqxmd.com/read/28438492/metabolism-of-14-c-octamethylcyclotetrasiloxane-14-c-d4-or-14-c-decamethylcyclopentasiloxane-14-c-d5-orally-gavaged-in-rainbow-trout-oncorhynchus-mykiss
#2
Jeanne Y Domoradzki, Jacob M Sushynski, Lisa M Thackery, Timothy A Springer, Timothy L Ross, Kent B Woodburn, Jeremy A Durham, Debra A McNett
Critical factors (uptake, distribution, metabolism and elimination) for understanding the bioaccumulation/biomagnification potential of Octamethylcyclotetrasiloxane (D4) and Decamethylcyclopentasiloxane (D5) siloxanes in fish were investigated to address whether these chemicals meet the "B" criteria of the Persistent, Bioaccumulative, and Toxic (PBT) classification. A metabolism study was conducted in rainbow trout whereby a 15mg [(14)C]D4 /kg bw or [(14)C]D5/kg bw as a single bolus oral dose was administered via gavage...
April 21, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28432942/insights-into-the-diversity-of-nod-like-receptors-identification-and-expression-analysis-of-nlrc3-nlrc5-and-nlrx1-in-rainbow-trout
#3
Claudio A Álvarez, Felipe Ramírez-Cepeda, Paula Santana, Elisa Torres, Jimena Cortés, Fanny Guzmán, Paulina Schmitt, Luis Mercado
Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) are efficient soluble intracellular sensors that activate defense mechanisms against pathogens. In teleost fish, the involvement of NLRs in the immune response is not well understood. However, recent work has evidenced the expression of different NLRs in response to some pathogen associated molecular patterns (PAMPs). In the present work, the cDNA sequence encoding three new NOD-like receptors were identified in Oncorhynchus mykiss, namely OmNLRC3, OmNLRC5 and OmNLRX1...
April 19, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28432152/exposure-to-seawater-increases-intestinal-motility-in-euryhaline-rainbow-trout-oncorhynchus-mykiss
#4
Jeroen Brijs, Grant W Hennig, Albin Gräns, Esmée Dekens, Michael Axelsson, Catharina Olsson
Upon exposure to seawater, euryhaline teleosts need to imbibe and desalinate seawater to allow for intestinal ion and water absorption, as this is essential for maintaining osmotic homeostasis. Despite the potential benefits of increased mixing and transport of imbibed water for increasing the efficiency of absorptive processes, the effect of water salinity on intestinal motility in teleosts remains unexplored. By qualitatively and quantitatively describing in vivo intestinal motility of euryhaline rainbow trout (Oncorhynchus mykiss), this study demonstrates that in freshwater, the most common motility pattern consisted of clusters of rhythmic, posteriorly propagating contractions that lasted ∼1-2 minutes followed by a period of quiescence lasting ∼4-5 minutes...
April 21, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28431210/exotic-gill-lice-species-copepoda-lernaeopodidae-salmincola-spp-infect-rainbow-trout-oncorhynchus-mykiss-and-brook-trout-salvelinus-fontinalis-in-the-southeastern-united-states
#5
Carlos F Ruiz, Jacob M Rash, Doug A Besler, Jackson R Roberts, Micah B Warren, Cova R Arias, Stephen A Bullard
Salmincola californiensis infected 25 of 31 (prevalence 0.8; intensity 2-35 [mean 6.6 ± standard deviation 7.7; n = 25]) rainbow trout, Oncorhynchus mykiss, from a private trout farm connected to the Watauga River, North Carolina. Salmincola edwardsii infected all of 9 (1.0; 2-43 [9.3 ± 13.0; 9]) brook trout, Salvelinus fontinalis, from Big Norton Prong, a tributary of the Little Tennessee River, North Carolina. Both lernaeopodids are well-known salmonid pathogens but neither is native to, nor has been previously taxonomically confirmed from, the southeastern United States...
April 21, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28430029/legal-mapping-analysis-of-state-telehealth-reimbursement-policies
#6
Kate E Trout, Sankeerth Rampa, Fernando A Wilson, Jim P Stimpson
BACKGROUND: There exists rapid growth and inconsistency in the telehealth policy environment, which makes it difficult to quantitatively evaluate the impact of telehealth reimbursement and other policies without the availability of a legal mapping database. INTRODUCTION: We describe the creation of a legal mapping database of state-level policies related to telehealth reimbursement of healthcare services. Trends and characteristics of these policies are presented...
April 21, 2017: Telemedicine Journal and E-health: the Official Journal of the American Telemedicine Association
https://www.readbyqxmd.com/read/28429822/what-goes-around-comes-around-an-investigation-of-resistance-to-proliferative-kidney-disease-in-rainbow-trout-oncorhynchus-mykiss-walbaum-following-experimental-re-exposure
#7
C Bailey, H Segner, T Wahli
Rainbow trout Oncorhynchus mykiss surviving proliferative kidney disease (PKD) are reported not to develop the disease upon re-exposure. However, the mechanisms involved in the immune response to re-exposure are unknown. We examined disease susceptibility and the immune response of naive 1+ rainbow trout when first exposed to Tetracapsuloides bryosalmonae in comparison with that of 1+ rainbow trout re-exposed to T. bryosalmonae. PKD pathogenesis, parasite burden and transcriptional signatures of the host immune response were assessed at 10, 25 and 40 d...
April 21, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28429604/intra-arterial-verapamil-post-thrombectomy-is-feasible-safe-and-neuroprotective-in-stroke
#8
Justin F Fraser, Michael Maniskas, Amanda Trout, Doug Lukins, Lindsey Parker, W Lane Stafford, Abdulnasser Alhajeri, Jill Roberts, Gregory J Bix
Large vessel ischemic stroke represents the most disabling subtype. While t-PA and endovascular thrombectomy can recanalize the occluded vessel, good clinical outcomes are not uniformly achieved. We propose that supplementing endovascular thrombectomy with superselective intra-arterial (IA) verapamil immediately following recanalization could be safe and effective. Verapamil, a calcium channel blocker, has been shown to be an effective IA adjunct in a pre-clinical mouse focal ischemia model. To demonstrate translational efficacy, mechanism, feasibility, and safety, we conducted a group of translational experiments...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28425498/a-new-phenomenon-sub-tg-solid-state-plasticity-induced-bonding-in-polymers
#9
Nikhil Padhye, David M Parks, Bernhardt L Trout, Alexander H Slocum
Polymer self-adhesion due to the interdiffusion of macromolecules has been an active area of research for several decades. Here, we report a new phenomenon of sub-Tg, solid-state, plasticity-induced bonding; where amorphous polymeric films were bonded together in a period of time on the order of a second in the solid-state at ambient temperatures, up to 60 K below their glass transition temperature (Tg), by subjecting them to active plastic deformation. Despite the glassy regime, the bulk plastic deformation triggered the requisite molecular mobility of the polymer chains, causing interpenetration across the interfaces held in contact...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425455/the-effect-of-hydraulic-flowback-and-produced-water-on-gill-morphology-oxidative-stress-and-antioxidant-response-in-rainbow-trout-oncorhynchus-mykiss
#10
Tamzin A Blewett, Alyssa M Weinrauch, Perrine L M Delompré, Greg G Goss
Hydraulic fracturing fluid are complex mixtures containing high concentrations of salts (up to 330,000 ppm), organic, and metal contaminants. However, little data exist on the potential mechanisms of toxicity of these flowback and produced wastewaters (FPW) on aquatic biota. Juvenile rainbow trout were exposed to either control, FPW (2.5 or 7.5%), FPW that had been treated with activated charcoal (AC), or a custom salt-matched control (SW; replicating only the salt content of FPW) for 48 hours. Gill histology revealed decreases in interlamellar cell mass (ILCM) and mean lamellar length in all treatments (FPW, AC and SW) compared to control, indicative of hyperosmotic stress...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421251/defining-the-ultrasound-longitudinal-natural-history-of-newly-diagnosed-pediatric-small-bowel-crohn-disease-treated-with-infliximab-and-infliximab-azathioprine-combination-therapy
#11
Jonathan R Dillman, Soudabeh Fazeli Dehkordy, Ethan A Smith, Michael A DiPietro, Ramon Sanchez, Vera DeMatos-Maillard, Jeremy Adler, Bin Zhang, Andrew T Trout
BACKGROUND: Little is known about changes in the imaging appearances of the bowel and mesentery over time in either pediatric or adult patients with newly diagnosed small bowel Crohn disease treated with anti-tumor necrosis factor-alpha (anti-TNF-α) therapy. OBJECTIVE: To define how bowel ultrasound findings change over time and correlate with laboratory inflammatory markers in children who have been newly diagnosed with pediatric small bowel Crohn disease and treated with infliximab...
April 18, 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/28421080/b-cell-functions-can-be-modulated-by-antimicrobial-peptides-in-rainbow-trout-oncorhynchus-mykiss-novel-insights-into-the-innate-nature-of-b-cells-in-fish
#12
Xu-Jie Zhang, Peng Wang, Nu Zhang, Dan-Dan Chen, Pin Nie, Jia-Le Li, Yong-An Zhang
B cells in fish were recently proven to have potent innate immune activities like macrophages. This inspired us to further explore the innate nature of B cells in fish. Moreover, antimicrobial peptides (AMPs) are representative molecules of innate immunity, and they can modulate the functions of macrophages. These make fish an appropriate model to study the interactions between B cells and AMPs. Interestingly, the results in this study revealed that the IgM(+) and IgT(+) B cells of rainbow trout could express multiple AMP genes, including four cathelicidin genes and one β-defensin gene...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28419978/dissolved-oxygen-stream-temperature-and-fish-habitat-response-to-environmental-water-purchases
#13
Sarah E Null, Nathaniel R Mouzon, Logan R Elmore
Environmental water purchases are increasingly used for ecological protection. In Nevada's Walker Basin (western USA), environmental water purchases augment streamflow in the Walker River and increase lake elevation of terminal Walker Lake. However, water quality impairments like elevated stream temperatures and low dissolved oxygen concentrations also limit ecosystems and species, including federally-threatened Lahontan cutthroat trout. In this paper, we prioritize water volumes and locations that most enhance water quality for riverine habitat from potential environmental water rights purchases...
April 15, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28419542/effect-of-nanosilver-on-metabolism-in-rainbow-trout-oncorhynchus-mykiss-an-investigation-using-different-respirometric-approaches
#14
Laura Murray, Michael D Rennie, Jon C Svendsen, Eva C Enders
Nanosilver (nAg) has been incorporated into many consumer products for its antimicrobial properties, including clothing and washing machines. Consequently, the potential for its release into aquatic environments is of significant concern. Documented toxic effects on fish include altered gene expression, gill damage, impaired gas exchange, as well as mortality at high nAg concentrations. This study reports the effects of nAg on the metabolism of Rainbow Trout (Oncorhynchus mykiss). Fish were exposed to environmentally-relevant concentrations (0...
April 17, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28419112/lens-metabolomic-profiling-as-a-tool-to-understand-cataractogenesis-in-atlantic-salmon-and-rainbow-trout-reared-at-optimum-and-high-temperature
#15
Sofie Charlotte Remø, Ernst Morten Hevrøy, Olav Breck, Pål Asgeir Olsvik, Rune Waagbø
Periods of high or fluctuating seawater temperatures result in several physiological challenges for farmed salmonids, including an increased prevalence and severity of cataracts. The aim of the present study was to compare cataractogenesis in Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss) reared at two temperatures, and investigate whether temperature influences lens metabolism and cataract development. Atlantic salmon (101±2 g) and rainbow trout (125±3 g) were reared in seawater at either 13°C (optimum for growth) or 19°C during the 35 days experiment (n = 4 tanks for each treatment)...
2017: PloS One
https://www.readbyqxmd.com/read/28415051/cross-interference-of-two-model-peroxisome-proliferators-in-peroxisomal-and-estrogenic-pathways-in-brown-trout-hepatocytes
#16
Tânia Vieira Madureira, Ivone Pinheiro, Fernanda Malhão, Célia Lopes, Ralph Urbatzka, L Filipe C Castro, Eduardo Rocha
Peroxisome proliferators cause species-specific effects, which seem to be primarily transduced by peroxisome proliferator-activated receptor alpha (PPARα). Interestingly, PPARα has a close interrelationship with estrogenic signaling, and this latter has already been promptly activated in brown trout primary hepatocytes. Thus, and further exploring this model, we assess here the reactivity of two PPARα agonists in direct peroxisomal routes and, in parallel the cross-interferences in estrogen receptor (ER) mediated paths...
April 6, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28414176/genome-specific-ppar%C3%AE-b-duplicates-in-salmonids-and-insights-into-estrogenic-regulation-in-brown-trout
#17
Tânia Vieira Madureira, Ivone Pinheiro, Rafaelle de Paula Freire, Eduardo Rocha, Luis Filipe Castro, Ralph Urbatzka
Peroxisome proliferator-activated receptors (PPAR) are key regulators of many processes in vertebrates, such as carbohydrate and lipid metabolism. PPARα, a member of the PPAR nuclear receptor gene subfamily (NR1C1), is involved in fatty acid metabolism, namely in peroxisomal β-oxidation. Two gene paralogues, pparαA and pparαB, were described in several teleost species with their origin dating back to the teleost-specific genome duplication (3R). Given the additional salmonid-specific genome duplication (4R), four genes could be theoretically anticipated for this gene subfamily...
April 14, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28410970/insulin-and-insulin-like-growth-factor-1-modulate-the-lipolytic-action-of-growth-hormone-by-altering-signal-pathway-linkages
#18
Heather E Bergan-Roller, Alicia T Ickstadt, Jeffrey D Kittilson, Mark A Sheridan
Growth hormone (GH) has many actions in vertebrates, including the regulation of two disparate metabolic processes: growth promotion (anabolic) and th e mobilization of stored lipids (catabolic). Our previous studies showed that GH stimulated IGF-1 production in hepatocytes from fed rainbow trout, but in cells from fasted fish GH stimulated lipolysis. In this study, we used rainbow trout (Oncorhynchus mykiss) to elucidate regulation of the mechanisms that enable cells to alter their lipolytic responsiveness to GH...
April 11, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28408684/draft-genome-sequence-of-the-fish-pathogen-flavobacterium-columnare-strain-csf-298-10
#19
Jason P Evenhuis, Scott E LaPatra, Joerg Graf
We announce here the draft genome assembly of Flavobacterium columnare CSF-298-10, a strain isolated from an outbreak of columnaris disease at a commercial trout farm in Hagerman Valley, Idaho, USA. The complete genome consists of 13 contigs totaling 3,284,579 bp, with an average G+C content of 31.5% and 2,933 predicted coding genes.
April 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28408167/purification-characterization-and-expression-of-transferrin-from-rainbow-trout-seminal-plasma
#20
Joanna Nynca, Mariola A Dietrich, Mikołaj Adamek, Dieter Steinhagen, Barbara Bilińska, Anna Hejmej, Andrzej Ciereszko
Transferrin (TF) is recognized as a multifunctional protein and has been implicated in antioxidative, antimicrobial protection, growth, differentiation and cytoprotection effects. An efficient, original three-step isolation procedure for TF consisting in hydrophobic interaction chromatography, gel filtration and preparative electrophoresis was developed. Rainbow trout TF was found to be N-glycosylated (not O-glycosylated) and phosphorylated at all serine, threonine, and tyrosine residues. The protein consists of several proteoforms with an average molecular weight of 76...
April 11, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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