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https://www.readbyqxmd.com/read/29675756/nodular-macroregenerative-tissue-as-a-pattern-of-regeneration-in-cholangiopathic-disorders
#1
Preston Roberts, Andrew T Trout, Jonathan R Dillman
BACKGROUND: Published case series have described central hepatic macroregenerative nodules or masses as a common feature of Alagille syndrome. Our experience suggests this regenerative pattern can be seen more generally in cholangiopathic disorders. OBJECTIVE: To define the frequency of central regenerative tissue in Alagille syndrome and other cholangiopathic disorders and to describe the typical appearance of such regenerative tissue. MATERIALS AND METHODS: We conducted a retrospective study of CT and MR imaging performed in children and young adults with cholangiopathic disorders between January 2000 and June 2016...
April 19, 2018: Pediatric Radiology
https://www.readbyqxmd.com/read/29671884/impact-of-pseudomonas-h6-surfactant-on-all-external-life-cycle-stages-of-the-fish-parasitic-ciliate-ichthyophthirius-multifiliis
#2
A Al-Jubury, C Lu, P W Kania, L von Gersdorff Jørgensen, Y Liu, I de Bruijn, J Raaijmakers, K Buchmann
A bacterial biosurfactant isolated from Pseudomonas (strain H6) has previously been shown to have a lethal effect on the oomycete Saprolegnia diclina infecting fish eggs. The present work demonstrates that the same biosurfactant has a strong in vitro antiparasitic effect on the fish pathogenic ciliate Ichthyophthirius multifiliis. Three life cycle stages (the infective theront stage, the tomont and the tomocyst containing tomites) were all susceptible to the surfactant. Theronts were the most sensitive showing 100% mortality in as low concentrations as 10 and 13 μg/ml within 30 min...
April 19, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29671811/shape-shifted-red-blood-cells-a-novel-red-blood-cell-stage
#3
Verónica Chico, Sara Puente-Marin, Iván Nombela, Sergio Ciordia, María Carmen Mena, Begoña Carracedo, Alberto Villena, Luis Mercado, Julio Coll, María Del Mar Ortega-Villaizan
Primitive nucleated erythroid cells in the bloodstream have long been suggested to be more similar to nucleated red cells of fish, amphibians, and birds than the red cells of fetal and adult mammals. Rainbow trout Ficoll-purified red blood cells (RBCs) cultured in vitro undergo morphological changes, especially when exposed to stress, and enter a new cell stage that we have coined shape-shifted RBCs (shRBCs). We have characterized these shRBCs using transmission electron microscopy (TEM) micrographs, Wright⁻Giemsa staining, cell marker immunostaining, and transcriptomic and proteomic evaluation...
April 19, 2018: Cells
https://www.readbyqxmd.com/read/29666522/development-of-a-novel-colorimetric-sensor-based-on-alginate-beads-for-monitoring-rainbow-trout-spoilage
#4
Marjan Majdinasab, Seyed Mohammad Hashem Hosseini, Marziyeh Sepidname, Manizheh Negahdarifar, Peiwu Li
Alginate is a non-toxic, renewable, and linear copolymer obtained from the brown algae Laminaria digitata that can be easily shaped into beads. Its good gel forming properties have made it useful for entrapping food and pharmaceutical ingredients. In this study, alginate beads were used in a novel application as a colorimetric sensor in food intelligent packaging. Colorimetric sensor was developed through entrapping red cabbage extract as a pH indicator in alginate beads. The pH indicator beads were used in rainbow trout packaging for monitoring fillets spoilage...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29665436/antimicrobial-resistance-and-molecular-characterization-of-pantoea-agglomerans-isolated-from-rainbow-trout-oncorhynchus-mykiss-fry
#5
Izzet Burcin Saticioglu, Muhammed Duman, Soner Altun
Aquaculture has become an important candidate as an animal protein source through its growth over the last decade. Based upon a report from the Food and Agriculture Organization of the United Nations, it is the fastest growing sector of the food industry, yet the pathogenicity of many biological agents involved in aquaculture is still unknown. In this study, we isolated Pantoe agglomerans from diseased rainbow trout on several occasions and also attempted to determine their phenotypic and genotypic characteristics, including antimicrobial resistance, of four bacterial isolates...
April 14, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29664968/water-system-is-a-controlling-variable-modulating-bacterial-diversity-of-gastrointestinal-tract-and-performance-in-rainbow-trout
#6
Omolola C Betiku, Carl J Yeoman, T Gibson Gaylord, Benjamin Americus, Sarah Olivo, Glenn C Duff, Wendy M Sealey
A two-phase feeding study evaluating performance of rainbow trout and comparing luminal and mucosal gastrointestinal tract (GIT) bacterial community compositions when fed two alternative protein diets in two rearing systems was conducted. Alternative protein diets (animal protein and plant protein diets) balanced with crystalline amino acids: lysine, methionine and threonine or unbalanced, were fed to rainbow trout in two separate water systems (recirculating (RR) and flow-through (FF)) for a period of 16 weeks...
2018: PloS One
https://www.readbyqxmd.com/read/29660722/determinants-of-mercury-contamination-in-viperine-snakes-natrix-maura-in-western-europe
#7
Jérémy Lemaire, Paco Bustamante, Anthony Olivier, Olivier Lourdais, Bruno Michaud, Alexandre Boissinot, Pedro Galán, François Brischoux
The effects of Hg contamination are presumably widespread across the components of aquatic ecosystems, but investigations have been mainly focused on freshwater fish, because this biota represents a major source of Hg for human populations. Yet, the possible bioaccumulation of Hg on other freshwater meso- and apex-predators (e.g., amphibians, reptiles) has been largely overlooked, especially in Western Europe. In this study, the determinants of Hg concentrations were assessed for the viperine snake (Natrix maura) across 6 populations (>130 individuals sampled in 2016 and 2017) in France and Spain...
April 13, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29660445/development-of-a-new-tool-for-the-long-term-in-vitro-ecotoxicity-testing-of-nanomaterials-using-a-rainbow-trout-cell-line-rtl-w1
#8
L Galbis-Martínez, M L Fernández-Cruz, L Alte, A Valdehita, I Rucandio, J M Navas
The current wide use of manufactured nanomaterials (MNs) is leading to the release of nanoparticles (NPs) to water bodies. Aquatic organisms, including fish, are exposed to low concentrations of NPs for long periods of time being necessary to develop laboratory toxicity tests reflecting realistic conditions. Additionally, today there is a demand of in vitro assays respecting the 3Rs principle. Thus, the main aim of this work was to stablish an in vitro tool for the assessment of long-term NPs ecotoxicity. Considering the key role of liver in detoxification, a rainbow trout liver cell line, RTL-W1, was used...
April 13, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29657746/a-framework-for-estimating-the-determinants-of-spatial-and-temporal-variation-in-vital-rates-and-inferring-the-occurrence-of-unobserved-extreme-events
#9
Simone Vincenzi, Dušan Jesenšek, Alain J Crivelli
We develop a general framework that combines long-term tag-recapture data and powerful statistical and modelling techniques to investigate how population, environmental and climate factors determine variation in vital rates and population dynamics in an animal species, using as a case study the population of brown trout living in Upper Volaja (Western Slovenia). This population has been monitored since 2004. Upper Volaja is a sink, receiving individuals from a source population living above a waterfall. We estimate the numerical contribution of the source population on the sink population and test the effects of temperature, population density and extreme events on variation in vital rates among 2647 individually tagged brown trout...
March 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29652184/dioxin-like-compounds-in-lake-fish-species-evaluation-by-dr-calux-bioassay
#10
S Sciuto, M Prearo, R Desiato, C Bulfon, E A V Burioli, G Esposito, C Guglielmetti, L Dell'atti, G Ru, D Volpatti, P L Acutis, F Martucci
Fish consumption is the principal source of intake of organochlorinated compounds in humans. Compared with other types of foods of animal origin, fish contain the highest levels of polychlorinated biphenyls (PCBs), polychlorinated dibenzo- p-dioxins, and polychlorinated dibenzofurans, all of which are classified as highly toxic organochlorine compounds. Currently, lakes and fish farms in northern Italy are not regularly monitored for PCBs and dioxins in areas contaminated by industrial sources, partially because of the high costs of traditional analytical methods that limit the number of samples to be analyzed...
April 13, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29649756/transcriptional-effects-of-phospholipid-fatty-acid-profile-on-rainbow-trout-liver-cells-exposed-to-methylmercury
#11
Aline Ferain, Chloé Bonnineau, Ineke Neefs, Krishna Das, Yvan Larondelle, Jean-François Rees, Cathy Debier, Benjamin Lemaire
Lipids, and their constitutive fatty acids, are key nutrients for fish health as they provide energy, maintain cell structure, are precursors of signalling molecules and act as nuclear receptor ligands. These specific roles may be of crucial importance in a context of exposure to pollutants. We recently showed that the fatty acid profile of rainbow trout liver cell phospholipids modulates sensitivity to an acute methylmercury challenge. In order to investigate mechanisms of effects, we herein tested whether specific polyunsaturated fatty acids (PUFAs) may protect cells from methylmercury through decreasing intracellular mercury accumulation and/or enhancing cellular defences (e...
March 22, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29643236/introduction-of-viral-haemorrhagic-septicaemia-virus-vhsv-to-freshwater-cultured-rainbow-trout-is-followed-by-bursts-of-adaptive-evolution
#12
Anna A Schönherz, Roald Forsberg, Bernt Guldbrandtsen, Albert J Buitenhuis, Katja Einer-Jensen
Viral haemorrhagic septicaemia virus (VHSV), a rhabdovirus infecting teleost fish, has repeatedly crossed the boundary from marine fish species to freshwater cultured rainbow trout. These naturally replicated cross-species transmission events permit the study of general and repeatable evolutionary events occurring in connection with viral emergence into a novel host species.The purpose of this study was to investigate adaptive molecular evolution of the VHSV glycoprotein, one of the key virus proteins involved in viral emergence, following emergence from marine species into freshwater cultured rainbow trout...
April 11, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29642539/in-silico-functional-networks-identified-in-fish-nucleated-red-blood-cells-by-means-of-transcriptomic-and-proteomic-profiling
#13
Sara Puente-Marin, Iván Nombela, Sergio Ciordia, María Carmen Mena, Verónica Chico, Julio Coll, María Del Mar Ortega-Villaizan
Nucleated red blood cells (RBCs) of fish have, in the last decade, been implicated in several immune-related functions, such as antiviral response, phagocytosis or cytokine-mediated signaling. RNA-sequencing (RNA-seq) and label-free shotgun proteomic analyses were carried out for in silico functional pathway profiling of rainbow trout RBCs. For RNA-seq, a de novo assembly was conducted, in order to create a transcriptome database for RBCs. For proteome profiling, we developed a proteomic method that combined: (a) fractionation into cytosolic and membrane fractions, (b) hemoglobin removal of the cytosolic fraction, (c) protein digestion, and (d) a novel step with pH reversed-phase peptide fractionation and final Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometric (LC ESI-MS/MS) analysis of each fraction...
April 9, 2018: Genes
https://www.readbyqxmd.com/read/29623085/non-native-minnows-threaten-quillwort-populations-in-high-mountain-shallow-lakes
#14
Esperança Gacia, Teresa Buchaca, Nayeli Bernal-Mendoza, Ibor Sabás, Enric Ballesteros, Marc Ventura
Submersed aquatic plants are a key component of shallow, clear water lakes contributing to primary production and water quality. High mountain lakes are naturally fishless although invasive trout and most recently minnows have been introduced causing a major impact on fauna richness. The Pyrenean high mountain range has preserved soft-water oligotrophic boreal isoetids in their southern limit of distribution but the recent fish introduction is a potential factor of stress that needs to be addressed. We here work under the hypothesis that due to contrasting ecological features, trout will not be heavily affecting quillwort populations while minnows will have a stronger effect on zooplankton and zoobenthos that will promote algal growth and reduce light availability for the underwater meadows...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29622666/behavioral-and-physiological-adaptations-to-high-flow-velocities-in-southwestern-native-chubs-gila-spp
#15
Clinton J Moran, Shannon P Gerry, Matthew W O'Neill, Caroline L Rzucidlo, Alice C Gibb
Morphological streamlining is often associated with physiological advantages for steady swimming in fishes. Though most commonly studied in pelagic fishes streamlining also occurs in fishes that occupy high flow environments. Before the installation of dams and water diversions, bonytail (Cyprinidae, Gila elegans ), a fish endemic to the Colorado River (USA), regularly experienced massive, seasonal flooding events. Individuals of G. elegans display morphological characteristics that may facilitate swimming in high-flow conditions, including a narrow caudal peduncle and a high aspect-ratio caudal fin...
April 5, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29621340/chinook-salmon-oncorhynchus-tshawytscha-genome-and-transcriptome
#16
Kris A Christensen, Jong S Leong, Dionne Sakhrani, Carlo A Biagi, David R Minkley, Ruth E Withler, Eric B Rondeau, Ben F Koop, Robert H Devlin
When unifying genomic resources among studies and comparing data between species, there is often no better resource than a genome sequence. Having a reference genome for the Chinook salmon (Oncorhynchus tshawytscha) will enable the extensive genomic resources available for Pacific salmon, Atlantic salmon, and rainbow trout to be leveraged when asking questions related to the Chinook salmon. The Chinook salmon's wide distribution, long cultural impact, evolutionary history, substantial hatchery production, and recent wild-population decline make it an important research species...
2018: PloS One
https://www.readbyqxmd.com/read/29621135/applying-fourier-transform-mid-infrared-spectroscopy-to-detect-the-adulteration-of-salmo-salar-with-oncorhynchus-mykiss
#17
Nuno Sousa, Maria João Moreira, Cristina Saraiva, José M M M de Almeida
The aim of this study was to evaluate the potential of Fourier transform infrared (FTIR) spectroscopy coupled with chemometric methods to detect fish adulteration. Muscles of Atlantic salmon ( Salmo salar ) (SS) and Salmon trout ( Onconrhynchus mykiss ) (OM) muscles were mixed in different percentages and transformed into mini-burgers. These were stored at 3 °C, then examined at 0, 72, 160, and 240 h for deteriorative microorganisms. Mini-burgers was submitted to Soxhlet extraction, following which lipid extracts were analyzed by FTIR...
April 5, 2018: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29614838/molecular-and-antigenic-characterization-of-piscine-orthoreovirus-prv-from-rainbow-trout-oncorhynchus-mykiss
#18
Kannimuthu Dhamotharan, Niccolò Vendramin, Turhan Markussen, Øystein Wessel, Argelia Cuenca, Ingvild B Nyman, Anne Berit Olsen, Torstein Tengs, Maria Krudtaa Dahle, Espen Rimstad
Piscine orthoreovirus (PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar ). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout ( Oncorhynchus mykiss ) with HSMI-like disease. PRV is considered to be an emerging pathogen in farmed salmonids. In this study, molecular and antigenic characterization of PRV-3 was performed. Erythrocytes are the main target cells for PRV, and blood samples that were collected from experimentally challenged fish were used as source of virus...
April 2, 2018: Viruses
https://www.readbyqxmd.com/read/29614402/fish-can-smell-trace-metals-at-environmentally-relevant-concentrations-in-freshwater
#19
Ebrahim Lari, Sarah J Bogart, Greg G Pyle
The objective of the present study was to investigate the ability of the olfactory system of rainbow trout (Oncorhynchus mykiss) to detect three trace metals, cadmium (Cd), copper (Cu), and nickel (Ni), using electro-olfactography (EOG). The olfactory response to all three metals was measured at either 10-6  M or at a concentration established by Alberta Environment and Parks (AEP) as the criterion for the protection of aquatic life. Results of the present study demonstrated that the olfactory system of rainbow trout can detect all three metals (i...
March 27, 2018: Chemosphere
https://www.readbyqxmd.com/read/29611504/albendazole-treatment-improves-work-capacity-in-women-smallholder-farmers-infected-with-hookworm-a-double-blind-randomized-control-trial
#20
Margaret Salmon, Christian Salmon, Maurice Masoda, Jean-Maurice Salumu, Carmine Bozzi, Phil Nieburg, Lisa M Harrison, Debbie Humphries, Naomi Abaca Uvon, Sarah K Wendel, Clint Trout, Michael Cappello
An estimated 4.7 billion people live in regions exposed to soil-transmitted helminths, intestinal parasites that have significant impacts on the health of women smallholder farmers. The goal of this trial was to determine whether treatment with albendazole impacts the work capacity of these farmers. This is a prospective double-blind, randomized effectiveness trial. Participants ( N = 250) were randomly selected from safe motherhood groups in the Democratic Republic of Congo. Prevalence/intensity of hookworm infection, hemoglobin, and demographics was obtained...
April 2, 2018: American Journal of Tropical Medicine and Hygiene
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