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https://www.readbyqxmd.com/read/29930054/genome-sequences-of-shewanella-baltica-and-shewanella-morhuae-strains-isolated-from-the-gastrointestinal-tract-of-freshwater-fish
#1
Daniel Castillo, Lone Gram, Frank E Dailey
We present here the genome sequences of Shewanella baltica strain CW2 and Shewanella morhuae strain CW7, isolated from the gastrointestinal tract of Salvelinus namaycush (lean lake trout) and Coregonus clupeaformis (whitefish), respectively. These genome sequences provide insights into the niche adaptation of these specific species in freshwater systems.
June 21, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29927879/no-requirement-for-targeted-theophylline-levels-for-diuretic-effect-of-aminophylline-in-critically-ill-children
#2
Katie Park, Lindsay C Trout, Cong Xu, Ming Wang, Robert F Tamburro, E Scott Halstead
OBJECTIVES: To determine the relationship between theophylline trough levels and urine output in critically ill children administered aminophylline as adjunctive diuretic therapy. DESIGN: Retrospective cohort study. SETTING: The PICU of a tertiary care children's hospital. PATIENTS: A mixed population of medical/surgical including postoperative cardiothoracic surgery patients less than 18 years old. INTERVENTIONS: Electronic medical records of all PICU patients admitted from July 2010 to June 2015 were reviewed, and patients who received aminophylline as diuretic therapy were identified...
June 20, 2018: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/29927021/a-geostatistical-state-space-model-of-animal-densities-for-stream-networks
#3
Daniel J Hocking, James T Thorson, Kyle O'Neil, Benjamin H Letcher
Population dynamics are often correlated in space and time due to correlations in environmental drivers as well as synchrony induced by individual dispersal. Many statistical analyses of populations ignore potential autocorrelations and assume that survey methods (distance and time between samples) eliminate these correlations, allowing samples to be treated independently. If these assumptions are incorrect, results and therefore inference may be biased and uncertainty under-estimated. We developed a novel statistical method to account for spatio-temporal correlations within dendritic stream networks, while accounting for imperfect detection in the surveys...
June 21, 2018: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/29926392/borax-supplementation-alleviates-hematotoxicity-and-dna-damage-in-rainbow-trout-oncorhynchus-mykiss-exposed-to-copper
#4
Gonca Alak, Veysel Parlak, Mehmet Enes Aslan, Arzu Ucar, Muhammed Atamanalp, Hasan Turkez
Heavy metals have harmful effects on health of both ecosystems and organisms to their accumulation ability. Copper (Cu) is an essential element for organism survival, but EPA considers Cu as a priority pollutant. On the other hand, boron has well-defined biological effects in living organisms including cytoprotection and genoprotection, although borax (BX) metabolism is poorly described in fish. Moreover, the effects of boron supplementation against Cu-induced hematotoxicity and DNA damage in aquatic organisms are still undetermined...
June 21, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29925575/implementation-of-duchenne-muscular-dystrophy-care-considerations
#5
Jennifer G Andrews, Kristin Conway, Christina Westfield, Christina Trout, F John Meaney, Katherine Mathews, Emma Ciafaloni, Christopher Cunniff, Deborah J Fox, Dennis Matthews, Shree Pandya
BACKGROUND: Duchenne muscular dystrophy (DMD) is an X-linked disorder characterized by progressive muscle weakness and multisystem involvement. Recent advances in management of individuals with DMD have prolonged survival. Lack of standardized care spurred an international collaboration to develop consensus-based care considerations for diagnosis and management. In this study, we evaluate adherence to considerations at selected sites. METHODS: We collaborated with the Muscular Dystrophy Surveillance, Tracking, and Research Network...
June 20, 2018: Pediatrics
https://www.readbyqxmd.com/read/29924322/hatchery-contributions-to-emerging-naturally-produced-lake-huron-lake-trout
#6
Kim Scribner, Iyob Tsehaye, Travis Brenden, Wendylee Stott, Jeannette Kanefsky, James Bence
Recent assessments indicate the emergence of naturally produced lake trout (Salvelinus namaycush) recruitment throughout Lake Huron in the North American Laurentian Great Lakes (>50% of fish <7 yrs). Because naturally produced fish derived from different stocked hatchery strains are unmarked, managers cannot distinguish strains contributing to natural recruitment. We used 15 microsatellite loci to identify strains of naturally produced lake trout (N=1567) collected in assessment fisheries during early (2002-2004) and late (2009-2012) sampling periods...
June 19, 2018: Journal of Heredity
https://www.readbyqxmd.com/read/29921219/genetic-and-transcriptomic-analyses-provide-new-insights-on-the-early-antiviral-response-to-vhsv-in-resistant-and-susceptible-rainbow-trout
#7
Eloi R Verrier, Carine Genet, Denis Laloë, Florence Jaffrezic, Andrea Rau, Diane Esquerre, Nicolas Dechamp, Céline Ciobotaru, Caroline Hervet, Francine Krieg, Luc Jouneau, Christophe Klopp, Edwige Quillet, Pierre Boudinot
BACKGROUND: The viral hemorrhagic septicemia virus (VHSV) is a major threat for salmonid farming and for wild fish populations worldwide. Previous studies have highlighted the importance of innate factors regulated by a major quantitative trait locus (QTL) for the natural resistance to waterborne VHSV infection in rainbow trout. The aim of this study was to analyze the early transcriptomic response to VHSV inoculation in cell lines derived from previously described resistant and susceptible homozygous isogenic lines of rainbow trout to obtain insights into the molecular mechanisms responsible for the resistance to the viral infection...
June 19, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29920385/modulation-of-rainbow-trout-oncorhynchus-mykiss-cutaneous-mucosal-immune-responses-following-anesthesia-a-comparative-study-on-different-anesthetic-agents
#8
Siyavash Soltanian, Seyed Hossein Hoseinifar, Amin Gholamhosseini
The present study investigated the possible effects of different anesthetic agents including MS222 (50 ppm), 2-Phenoxyethanol (2-PE) (0.2 mL L-1 ) and clove oil (25 ppm), on cutaneous mucosal immune parameters in rainbow trout (Oncorhynchus mykiss). The induction and recovery times for each anesthetic agent were assessed. Also, the immune parameters were measured in skin mucus, 1 and 24 h post anesthesia. No significant difference was observed among treatments at 1 h post-anesthesia except for bactericidal and alkaline phosphatase (ALP) activities which was significantly enhanced in fish exposed to 2-PE compared to other anesthetics...
June 16, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29913574/inferring-watershed-hydraulics-and-cold-water-habitat-persistence-using-multi-year-air-and-stream-temperature-signals
#9
Martin A Briggs, Zachary C Johnson, Craig D Snyder, Nathaniel P Hitt, Barret L Kurylyk, Laura Lautz, Dylan J Irvine, Stephen T Hurley, John W Lane
Streams strongly influenced by groundwater discharge may serve as "climate refugia" for sensitive species in regions of increasingly marginal thermal conditions. The main goal of this study is to develop paired air and stream water annual temperature signal analysis techniques to elucidate the relative groundwater contribution to stream water and the effective groundwater flowpath depth. Groundwater discharge to streams attenuates surface water temperature signals, and this attenuation can be diagnostic of groundwater gaining systems...
September 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29913320/assessing-the-role-of-the-acid-sensing-ion-channel-asic4b-in-sodium-uptake-by-larval-zebrafish
#10
Alex M Zimmer, Agnieszka K Dymowska, Yusuke Kumai, Greg G Goss, Steve F Perry, Raymond W M Kwong
Na+ uptake in larval zebrafish (Danio rerio) is coordinated by three mechanisms: Na+ /H+ -exchanger 3b (NHE3b) expressed in H+ -ATPase-rich (HR) cells, an unidentified Na+ channel coupled to electrogenic H+ -ATPase expressed in HR cells, and Na+ -Cl- -cotransporter (NCC) expressed in NCC cells. Recently, acid-sensing ion channels (ASICs) were proposed to be the putative Na+ channel involved in H+ -ATPase-mediated Na+ uptake in adult zebrafish and rainbow trout. In the present study, we hypothesized that ASICs also play this role in Na+ uptake in larval zebrafish...
June 15, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29913268/the-in-vitro-toxicity-analysis-of-titanium-dioxide-tio-2-nanoparticles-on-kinematics-and-biochemical-quality-of-rainbow-trout-sperm-cells
#11
Mustafa Erkan Özgür, Sevgi Balcıoğlu, Ahmet Ulu, İmren Özcan, Fatih Okumuş, Süleyman Köytepe, Burhan Ateş
In recent years, titanium dioxide (TiO2 ) nanoparticles (NPs) as metal oxide nanoparticles are widely used in industry, agriculture, personal care products, cosmetics, sun protection and toothpaste, electronics, foodstuffs and food packaging. This use of nano-TiO2 has been associated with environmental toxicity concerns. Therefore, the aim of this study was to evaluate the in vitro effect of different doses of TiO2 NPs (∼30-40 nm) (0.01, 0.1, 0.5, 1, 10 and 50 mg/L) at 4oC for 3 h on the sperm cell kinematics as velocities of Rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) sperm cells...
June 12, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29909333/evaluation-of-architectural-and-histopathological-biomarkers-in-the-intestine-of-brown-trout-salmo-trutta-linnaeus-1758-challenged-with-environmental-pollution
#12
Josip Barišić, Vlatka Filipović Marijić, Tatjana Mijošek, Rozelindra Čož-Rakovac, Zrinka Dragun, Nesrete Krasnići, Dušica Ivanković, Dáša Kružlicová, Marijana Erk
In the present study novel histopathological approach, using fish intestine as a sensitive bioindicator organ of pollution impact in the freshwater ecosystem, was proposed. Histopathological alterations were compared between native brown trout (Salmo trutta Linnaeus, 1758) from the reference (Krka River spring) and pollution impacted location (influence of technological/municipal wastewaters and agricultural runoff near the Town of Knin) of the karst Krka River in Croatia. In brown trout from both locations, severe parasitic infestation with acanthocephalan species Dentitruncus trutae was found, enabling evaluation of acanthocephalan infestation histopathology, which indicated parasite tissue reaction in a form of inflammatory, necrotic and hyperplastic response that extended throughout lamina epithelialis mucosae, lamina propria, and lamina muscularis mucosae...
June 14, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29906393/bisphenol-a-in-eggs-impairs-the-long-term-stress-performance-of-rainbow-trout-in-two-generations
#13
Jith Thomas, Oana Birceanu, Bastien Sadoul, Mathilakath M Vijayan
Salmonids are ecologically, economically and culturally important fish species in North America, but whether contaminants in the environment may play a role in their population decline is unclear. We tested the hypothesis that BPA deposition in eggs, mimicking a maternal transfer scenario, compromises the stress axis functioning and target tissues stress response in two generations of a model salmonid species, the rainbow trout (Oncorhynchus mykiss). Eggs were enriched with 0, 4 or 40 ng BPA, fertilized, and reared in clean water for two generations...
June 15, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29904385/dysregulation-of-b-cell-activity-during-proliferative-kidney-disease-in-rainbow-trout
#14
Beatriz Abos, Itziar Estensoro, Pedro Perdiguero, Marc Faber, Yehfang Hu, Patricia Díaz Rosales, Aitor G Granja, Christopher J Secombes, Jason W Holland, Carolina Tafalla
Proliferative kidney disease (PKD) is a widespread disease caused by the endoparasite Tetracapsuloides bryosalmonae (Myxozoa: Malacosporea). Clinical disease, provoked by the proliferation of extrasporogonic parasite stages, is characterized by a chronic kidney pathology with underlying transcriptional changes indicative of altered B cell responses and dysregulated T-helper cell-like activities. Despite the relevance of PKD to European and North American salmonid aquaculture, no studies, to date, have focused on further characterizing the B cell response during the course of this disease...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29903823/draft-genome-sequence-of-fish-pathogen-aeromonas-bestiarum-ga97-22
#15
Salih Kumru, Hasan C Tekedar, Matt J Griffin, Geoffrey C Waldbieser, Mark R Liles, Tad Sonstegard, Steven G Schroeder, Mark L Lawrence, Attila Karsi
Aeromonas bestiarum is a Gram-negative mesophilic motile bacterium causing acute hemorrhagic septicemia or chronic skin ulcers in fish. Here, we report the draft genome sequence of A. bestiarum strain GA97-22, which was isolated from rainbow trout in 1997. This genome sequence will improve our understanding of the complex taxonomy of motile aeromonads.
June 14, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29897970/consumption-of-carotenoids-not-increased-by-bacterial-infection-in-brown-trout-embryos-salmo-trutta
#16
Lucas Marques da Cunha, Laetitia G E Wilkins, Laure Menin, Daniel Ortiz, Véronique Vocat-Mottier, Claus Wedekind
Carotenoids are organic pigment molecules that play important roles in signalling, control of oxidative stress, and immunity. Fish allocate carotenoids to their eggs, which gives them the typical yellow to red colouration and supports their resistance against microbial infections. However, it is still unclear whether carotenoids act mainly as a shield against infection or are used up during the embryos' immune defence. We investigated this question with experimental families produced from wild-caught brown trout (Salmo trutta)...
2018: PloS One
https://www.readbyqxmd.com/read/29891141/assessing-concentration-of-antibiotics-in-tissue-during-oral-treatments-against-piscirickettsiosis
#17
D Price, J Sánchez, J McClure, S McConkey, R Ibarra, S St-Hilaire
The use of antimicrobials in aquaculture is increasingly being scrutinized. In Chile, piscirickettsiosis accounts for approximately 90% of the total volume of antibiotics used in marine aquaculture. Treatment failures are frequently reported, but there is limited information on why this occurs. Fish producers have started assessing the level of antibiotics in fish tissues during and immediately after in-feed treatments to determine if they are adequately medicating their fish. In this study, we evaluated the probability of finding antibiotic concentrations in muscle tissue above the minimum inhibitory concentration for 90% of the P...
August 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29890942/correction-to-de-novo-transcriptome-assembly-annotation-and-comparison-of-four-ecological-and-evolutionary-model-salmonid-fish-species
#18
Madeleine Carruthers, Andrey A Yurchenko, Julian J Augley, Colin E Adams, Pawel Herzyk, Kathryn R Elmer
Following the publication of this article [1], the authors noticed found that they incorrectly reported the BUSCO completeness for the PhyloFish brown trout and European whitefish transcriptomes. This was due to an error in their TransDecoder pipeline and restricted to those two datasets and their interpretation. They apologise for this misreported result and thank the authors of the PhyloFish database for bringing it to their attention.
June 11, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29887855/temperature-dependent-gene-expression-in-yersinia-ruckeri-tracking-specific-genes-by-bioluminescence-during-in-vivo-colonization
#19
Jessica Mendez, Desirée Cascales, Ana I Garcia-Torrico, Jose A Guijarro
Yersinia ruckeri is a bacterium causing fish infection processes at temperatures below the optimum for growth. A derivative Tn5 transposon was used to construct a library of Y. ruckeri mutants with transcriptional fusions between the interrupted genes and the promoterless luxCDABE and lacZY operons. In vitro analysis of β-galactosidase activity allowed the identification of 168 clones having higher expression at 18°C than at 28°C. Among the interrupted genes a SAM-dependent methyltransferase, a diguanylated cyclase, three genes involved in legionaminic acid synthesis and three transcriptional regulators were defined...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29886079/endogenous-regulation-of-11-deoxycorticosterone-doc-and-corticosteroid-receptors-crs-during-rainbow-trout-early-development-and-the-effects-of-corticosteroids-on-hatching
#20
Pia Kiilerich, Benjamin Geffroy, Claudiane Valotaire, Patrick Prunet
Clear evidence for a physiological role of the mineralocorticoid-like hormone 11-deoxycorticosterone (DOC) and the mineralocorticoid receptor (MR) in fish is still lacking. Efforts to demonstrate an osmoregulatory role for this hormone has so far not been conclusive, while a few scattered studies have indicated a role for DOC in development and reproduction. In this study, we investigate the onset of de novo DOC synthesis in parallel with endogenous corticosteroid receptor mRNA production from fertilization to the swim-up stage in rainbow trout...
June 7, 2018: General and Comparative Endocrinology
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