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https://www.readbyqxmd.com/read/28345192/respirometry-increases-cortisol-levels-in-rainbow-trout-oncorhynchus-mykiss-implications-for-measurements-of-metabolic-rate
#1
L Murray, M D Rennie, J C Svendsen, E C Enders
This study aimed to assess the extent to which chasing, handling and confining Oncorhynchus mykiss to a small respirometer chamber during respirometric experiments is stressful and affects metabolic measurements. The study observed increased cortisol levels in animals tested using a chase protocol and subsequent intermittent-flow respirometry, suggesting that this procedural treatment may stress animals.
March 27, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28343776/a-effective-dna-vaccine-against-diverse-genotype-j-infectious-hematopoietic-necrosis-virus-strains-prevalent-in-china
#2
Liming Xu, Jingzhuang Zhao, Miao Liu, Gael Kurath, Guangming Ren, Scott E Lapatra, Jiasheng Yin, Hongbai Liu, Jian Feng, Tongyan Lu
Infectious hematopoietic necrosis virus (IHNV) is the most important pathogen threatening the aquaculture of salmonid fish in China. In this study, a DNA vaccine, designated pIHNch-G, was constructed with the glycoprotein (G) gene of a Chinese IHNV isolate SD-12 (also called Sn1203) of genotype J. The minimal dose of vaccine required, the expression of the Mx-1 gene in the muscle (vaccine delivery site) and anterior kidney, and the titers of the neutralizing antibodies produced were used to evaluate the vaccine efficacy...
March 23, 2017: Vaccine
https://www.readbyqxmd.com/read/28343271/protein-degradation-systems-in-the-skeletal-muscles-of-parr-and-smolt-atlantic-salmon-salmo-salar-l-and-brown-trout-salmo-trutta-l
#3
Nadezda P Kantserova, Liudmila A Lysenko, Alexey E Veselov, Nina N Nemova
Although protein degradation limits the rate of muscle growth in fish, the role of proteolytic systems responsible for degrading myofibrillar proteins in skeletal muscle is not well defined. The study herein aims to evaluate the role of calpains (calcium-activated proteases) and proteasomes (ATP-dependent proteases) in mediating muscle protein turnover at different life stages in wild salmonids. Protease activities were estimated in Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.) parr and smolts from the Indera River (Kola Peninsula, Russia)...
March 26, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28343146/antimicrobial-effect-of-the-biotronic%C3%A2-top3-supplement-and-efficacy-in-protecting-rainbow-trout-oncorhynchus-mykiss-from-infection-by-aeromonas-salmonicida-subsp-salmonicida
#4
Simon Menanteau-Ledouble, Ines Krauss, Rui Alexandre Goncalves, Barbara Weber, Gonçalo Abreu Santos, Mansour El-Matbouli
Demand for more environmentally friendly practices have led to the adoption of several feed supplements by the fish farming industry. In the present study, we investigated a commercially available formula that includes a mixture of three compounds: organic acids, a phytochemical and Biomin® Permeabilizing Complex. This mixture demonstrated antimicrobial properties in vitro and was able to inhibit growth of multiple species of aquatic bacterial pathogens, including Aeromonas salmonicida. Bacterial challenge was performed using A...
March 18, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28342022/identifying-connexin-expression-and-determining-gap-junction-intercellular-communication-in-rainbow-trout-cells
#5
Joshua Hooper, Sarah J Poynter, Stephanie J DeWitte-Orr
Gap junctions are groups of membrane-bound channels that allow the passage of small molecules and ions between cells, permitting cell-cell communication. Because of their importance in cell homeostasis, gap junction presence and function were characterized in three commonly studied rainbow trout cell lines, namely RTgill-W1, RTgutGC, and RTG-2. Firstly, gap junction presence was determined by screening for gap junction protein alpha 7 and alpha 1 (GJA7 and GJA1) presence at the transcript level and GJA7 at the protein level...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28341353/the-nck-and-abi-adaptor-genes-in-catfish-and-their-involvement-in-esc-disease-response
#6
Tao Zhou, Ning Li, Shikai Liu, Yulin Jin, Qiang Fu, Sen Gao, Yang Liu, Zhanjiang Liu
Adaptor proteins non-catalytic region of tyrosine kinase (NCK) and Abelson interactor (ABI) are crucial for disease response. NCK1 was identified to be a candidate gene for enteric septicemia of catfish (ESC) disease resistance, and was speculated to play similar roles during ESC and enteropathogenic Escherichia coli (EPEC) pathogenicity. ABI1 was reported as a positional candidate gene for bacterial cold water disease (BCWD) resistance in rainbow trout. In this study, three NCK genes and six ABI genes were identified in the channel catfish (Ictalurus punctatus) genome and blue catfish (I...
March 22, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28339310/investigations-of-octylglyceryl-dextran-graft-poly-lactic-acid-nanoparticles-for-peptide-delivery-to-the-brain
#7
Asme Boussahel, Daniel M Ibegbu, Rhita Lamtahri, Julie Maucotel, Julien Chuquet, Benjamin Lefranc, Jérôme Leprince, Marta Roldo, Jean-Claude Le Mével, Darek Gorecki, Eugen Barbu
AIM: Develop modified dextran nanoparticles showing potential to assist with drug permeation across the blood-brain barrier for the delivery of neuropeptides. METHODS: Nanoparticles loaded by emulsification with model macromolecular actives were characterized in terms of stability, cytotoxicity and drug-release behavior. Peptide-loaded nanoformulations were tested in an in vivo trout model and in food-deprived mice. RESULTS: Nanoformulations loaded with model peptides showed good stability and appeared nontoxic in low concentration against human brain endothelial cells...
March 24, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28338007/human-immunodeficiency-virus-type-1-myeloid-derived-suppressor-cells-inhibit-cytomegalovirus-inflammation-through-interleukin-27-and-b7-h4
#8
Ankita Garg, Rodney Trout, Stephen A Spector
HIV/CMV co-infected persons despite prolonged viral suppression often experience persistent immune activation, have an increased frequency of myeloid derived suppressor cells (MDSC) and are at increased risk for cardiovascular disease. We examined how HIV MDSC control CD4(+) T cell IFNγ response to a CMVpp65 peptide pool (CMVpp65). We show that HIV/CMV co-infected persons with virologic suppression and recovered CD4(+) T cells compared to HIV(-)/CMV(+) controls exhibit an increase in CD4(+)CX3CR1(+)IFNγ(+) cells in response to CMVpp65; MDSC depletion further augmented CD4(+)CX3CR1(+)IFNγ(+) cells and IFNγ production...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337034/exposure-to-an-acute-hypoxic-stimulus-during-early-life-affects-the-expression-of-glucose-metabolism-related-genes-at-first-feeding-in-trout
#9
Jingwei Liu, Elisabeth Plagnes-Juan, Inge Geurden, Stéphane Panserat, Lucie Marandel
Rainbow trout (Oncorhynchus mykiss) is considered a "glucose-intolerant" species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L(-1) O2) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330969/influence-of-the-coronary-circulation-on-thermal-tolerance-and-cardiac-performance-during-warming-in-rainbow-trout
#10
Andreas Ekström, Michael Axelsson, Albin Gräns, Jeroen Brijs, Erik Sandblom
Thermal tolerance in fish may be related to an oxygen limitation of cardiac function. While the hearts of some fish species receive oxygenated blood via a coronary circulation, the influence of this oxygen supply on thermal tolerance and cardiac performance during warming remain unexplored. Here, we analyzed the effect in vivo of acute warming on coronary blood flow in adult sexually mature rainbow trout (Onchorhynchus mykiss), and the consequences of chronic coronary ligation on cardiac function and thermal tolerance in juvenile trout...
February 22, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28330807/dietary-myrtle-myrtus-communis-l-improved-non-specific-immune-parameters-and-bactericidal-activity-of-skin-mucus-in-rainbow-trout-oncorhynchus-mykiss-fingerlings
#11
Hadis Mansouri Taee, Abdolmajid Hajimoradloo, Seyed Hossein Hoseinifar, Hassan Ahmadvand
The present study examined the effects of dietary Myrtle (Myrtus communis L.) on non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (Oncorhynchus mykiss) fingerlings. Three hundred and sixty fingerlings (6.50 ± 0.55 g (were distributed in twelve cages (65 × 65 × 65 cm) with a metal framework. The study included four treatments repeated in triplicates. The treatments were feeding trouts with experimental diets containing different levels (0, 0.5, 1 and 1.5%) of Myrtle powder...
March 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28323891/characterization-of-baff-and-april-subfamily-receptors-in-rainbow-trout-oncorhynchus-mykiss-potential-role-of-the-baff-april-axis-in-the-pathogenesis-of-proliferative-kidney-disease
#12
Aitor G Granja, Jason W Holland, Jaime Pignatelli, Christopher J Secombes, Carolina Tafalla
Proliferative kidney disease (PKD) is a parasitic infection of salmonid fish characterized by hyper-secretion of immunoglobulins in response to the presence of the myxozoan parasite, Tetracapsuloides bryosalmonae. In this context, we hypothesized that the BAFF/APRIL axis, known to play a major role in B cell differentiation and survival in mammals, could be affected by the parasite and consequently be involved in the apparent shift in normal B cell activity. To regulate B cell activity, BAFF and APRIL bind to transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) and B cell maturation antigen (BCMA), whereas BAFF also binds to BAFF receptor (BAFF-R)...
2017: PloS One
https://www.readbyqxmd.com/read/28323368/biotic-interactions-modify-multiple-stressor-effects-on-juvenile-brown-trout-in-an-experimental-stream-food-web
#13
Andreas Bruder, Romana K Salis, Peter E Jones, Christoph D Matthaei
Agricultural land use results in multiple stressors affecting stream ecosystems. Flow reduction due to water abstraction, elevated levels of nutrients and chemical contaminants are common agricultural stressors worldwide. Concurrently, stream ecosystems are also increasingly affected by climate change. Interactions among multiple co-occurring stressors result in biological responses that cannot be predicted from single-stressor effects (i.e. synergisms and antagonisms). At the ecosystem level, multiple-stressor effects can be further modified by biotic interactions (e...
March 21, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28322206/comparative-study-of-proliferative-kidney-disease-in-grayling-thymallus-thymallus-and-brown-trout-salmo-trutta-fario-an-exposure-experiment
#14
Heike Schmidt-Posthaus, Albert Ros, Regula Hirschi, Ernst Schneider
Proliferative kidney disease (PKD) is an emerging disease threatening wild salmonid populations, with the myxozoan parasite Tetracapsuloides bryosalmonae as the causative agent. Species differences in parasite susceptibility and disease-induced mortality seem to exist. The aim of the present study was to compare incidence, pathology and mortality of PKD in grayling Thymallus thymallus and brown trout Salmo trutta under identical semi-natural conditions. Young-of-the-year grayling and brown trout, free of T...
March 21, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28317779/development-and-validation-of-a-lc-ms-ms-method-for-the-determination-of-isoeugenol-in-finfish
#15
Lin Ye
A simple, rapid, selective and sensitive LC-MS/MS method was developed and validated for the determination of isoeugenol in finfish using an off-line dansylation derivatization step to enhance signal intensity. The sample was extracted with 10mL acetone using a tissue homogenizer, followed by derivatization with dansyl chloride and analysis by liquid chromatography tandem mass spectrometry. The method detection limit ranged from 0.2 to 0.7ngg(-1) in six fish species. The limit of quantification was 2.5ngg(-1) in each species...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317020/single-cell-reconstruction-of-oxytocinergic-neurons-reveals-separate-hypophysiotropic-and-encephalotropic-subtypes-in-larval-zebrafish
#16
Ulrich Herget, Jose Arturo Gutierrez-Triana, Oriana Salazar Thula, Boris Knerr, Soojin Ryu
Oxytocin regulates a diverse set of processes including stress, analgesia, metabolism, and social behavior. How such diverse functions are mediated by a single hormonal system is not well understood. Different functions of oxytocin could be mediated by distinct cell groups, yet it is currently unknown whether different oxytocinergic cell types exist that specifically mediate peripheral neuroendocrine or various central neuromodulatory processes via dedicated pathways. Using the Brainbow technique to map the morphology and projections of individual oxytocinergic cells in the larval zebrafish brain, we report here the existence of two main types of oxytocinergic cells: those that innervate the pituitary and those that innervate diverse brain regions...
January 2017: ENeuro
https://www.readbyqxmd.com/read/28314140/an-investigation-of-the-immunotoxicity-of-oil-sands-processed-water-and-leachates-in-trout-leukocytes
#17
F Gagné, A Bruneau, P Turcotte, C Gagnon, E Lacaze
Increased oil sands (OS) mining activity has raised concerns about impacts on aquatic organisms. This study sought to examine the effects of single representative compounds from OS (benzo(a)pyrene, naphthalene), a mixture of naphthenic acids (NAs), OS-processed water (OSPW) and OS leachate (OSL) extracts on rainbow trout leukocytes. Primary cultures of trout leukocytes were exposed to increasing concentrations of benzo(a)pyrene, naphthalene, NAs, OSPW and OSL for 48h at 18°C. Immunocompetence was followed by measuring changes in lymphocyte and macrophage viability and phagocytosis...
March 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28303530/a-randomized-open-label-multicenter-phase-ii-study-evaluating-the-efficacy-and-safety-of-bth1677-1-3-1-6-beta-glucan-imprime-pgg-in-combination-with-cetuximab-and-chemotherapy-in-patients-with-advanced-non-small-cell-lung-cancer
#18
M Thomas, P Sadjadian, J Kollmeier, J Lowe, P Mattson, J R Trout, M Gargano, M L Patchen, R Walsh, M Beliveau, J F Marier, N Bose, K Gorden, F Schneller
Introduction BTH1677, a 1,3-1,6 beta-glucan immunomodulator, stimulates a coordinated anti-cancer immune response in combination with anti-tumor antibody therapies. This phase II study explored the efficacy, pharmacokinetics (PK), and safety of BTH1677 combined with cetuximab/carboplatin/paclitaxel in untreated stage IIIB/IV non-small cell lung cancer (NSCLC) patients. Methods Patients were randomized 2:1 to the BTH1677 arm (N=60; BTH1677, 4 mg/kg, weekly; cetuximab, initial dose 400 mg/m(2) and subsequent doses 250 mg/m(2), weekly; carboplatin, 6 mg/mL/min AUC (area-under-the-curve) by Calvert formula, once each 3-week cycle [Q3W]); and paclitaxel, 200 mg/m(2), Q3W) or Control arm (N=30; cetuximab/carboplatin/paclitaxel as above)...
March 16, 2017: Investigational New Drugs
https://www.readbyqxmd.com/read/28302579/the-baff-april-axis-plays-an-important-role-in-virus-induced-peritoneal-responses-in-rainbow-trout
#19
Irene Soleto, Beatriz Abós, Rosario Castro, Lucia González, Carolina Tafalla, Aitor G Granja
IgM(+) B cells have been recently demonstrated to be key regulators of peritoneal inflammation in teleost, as a large number of them occupy the peritoneal cavity after 48 h of antigenic stimulation. Despite this, the number of studies addressing the mechanism through which this cell population expands and differentiates in response to stimuli has been scarcely addressed. Because the BAFF/APRIL axis is known to play a major role in B cell survival and differentiation in mammals, we hypothesized that it could be affected in the peritoneal cavity in response to an inflammatory stimulus...
March 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28302378/hypothermic-storage-of-isolated-spermatogonia-and-oogonia-from-rainbow-trout-oncorhynchus-mykiss
#20
Bahram Falahatkar, Samaneh Poursaeid, Ryota Kitada, Goro Yoshizaki
A growing number of fish species are endangered due to human activities. A short- or long-time preservation of gametes could conserve genetic resources of threatened fish species. The aim of this study was to evaluate a hypothermic condition for short-term preservation of spermatogonia and oogonia cells isolated from immature transgenic rainbow trout, Oncorhynchus mykiss, and to determine the maximum time point for further transplantation. Viability rate of germ cells was investigated after isolation and during storage at 4 °C up to 24 h...
March 13, 2017: Cryobiology
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