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Anna Baranik, Rafal Sitko, Anna Gagor, Ignasi Queralt, Eva Margui, Beata Zawisza
Graphene oxide decorated with cerium (IV) oxide (GO/CeO2) was synthesized and applied in adsorption of several metal ions such as As(III), As(V), Se(IV), Cu(II) and Pb(II) from aqueous samples. The important feature of GO/CeO2 nanocomposite is also its selectivity towards selenite in the presence of selenate. The structure of GO/CeO2 has been proven by microscopic and spectroscopic techniques. The maximum adsorption capacities of GO/CeO2 calculated by Langmuir model towards arsenic, selenium, copper and lead ions are between 6 and 30 mg g-1...
February 18, 2018: Analytical Chemistry
M Angeles Argudín, A Deplano, S Vandendriessche, M Dodémont, C Nonhoff, O Denis, S Roisin
Studies based on genome-wide single nucleotide polymorphisms (SNPs) supported the existence of two subpopulations in clonal complex (CC) 398 Staphylococcus aureus: an ancestral human-adapted clade (HC) and an animal-associated clade (AC). In this study, we have investigated the occurrence of genetic markers that allow discrimination of these subpopulations among CC398 isolates collected during 2014 to 2016 from human patients in Belgium. A collection of isolates was investigated by means of spa-typing and 16S-mecA-nuc PCR...
February 15, 2018: European Journal of Clinical Microbiology & Infectious Diseases
D Heinrich, S Bohnacker, M A Nasseri, N Feucht, C P Lohmann, M Maier
BACKGROUND: High-resolution spectral domain optical coherence tomography (SD-OCT) is a gold standard technique for diagnosis, management and monitoring of patients with vitreoretinal diseases. Preoperative diagnostics for evaluation of intraocular and retinal status in patients with vitreous haemorrhage are limited, thus final therapeutic decisions can only be made during explorative vitrectomy. We evaluated the use of intraoperative SD-OCT (iSD-OCT) as a real-time additional diagnostic tool during explorative vitrectomy in patients with vitreous haemorrhage...
February 15, 2018: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
Stanimir Kambarev, Frédéric Pecorari, Stéphane Corvec
Background: Streptococcus gallolyticus ssp. gallolyticus (Sgg) is a commensal bacterium and an opportunistic pathogen. In humans it has been clinically associated with the incidence of colorectal cancer (CRC) and epidemiologically recognized as an emerging cause of infective endocarditis (IE). The standard therapy of Sgg includes the administration of a penicillin in combination with an aminoglycoside. Even though penicillin-resistant isolates have still not been reported, epidemiological studies have shown that this microbe is a reservoir of multiple acquired genes, conferring resistance to tetracyclines, aminoglycosides, macrolides and glycopeptides...
February 9, 2018: Journal of Antimicrobial Chemotherapy
Isabel Hottmann, Valentina M T Mayer, Markus B Tomek, Valentin Friedrich, Matthew B Calvert, Alexander Titz, Christina Schäffer, Christoph Mayer
Tannerella forsythia is an anaerobic, Gram-negative oral pathogen that thrives in multispecies gingival biofilms associated with periodontitis. The bacterium is auxotrophic for the commonly essential bacterial cell wall sugar N-acetylmuramic acid (MurNAc) and, thus, strictly depends on an exogenous supply of MurNAc for growth and maintenance of cell morphology. A MurNAc transporter (Tf_MurT; Tanf_08375) and an ortholog of the Escherichia coli etherase MurQ (Tf_MurQ; Tanf_08385) converting MurNAc-6-phosphate to GlcNAc-6-phosphate were recently described for T...
2018: Frontiers in Microbiology
Carlos Neira, Melissa Vales, Guillermo Mendoza, Eunha Hoh, Lisa A Levin
Polychlorinated biphenyl (PCB) concentrations were determined in surface sediments from three recreational marinas in San Diego Bay, California. Total PCB concentrations ranged from 23 to 153, 31-294, and 151-1387ngg-1 for Shelter Island Yacht Basin (SIYB), Harbor Island West (HW) and Harbor Island East (HE), respectively. PCB concentrations were significantly higher in HE and PCB group composition differed relative to HW and SIYB, which were not significantly different from each other in concentration or group composition...
January 2018: Marine Pollution Bulletin
Abdullah Ozkaya, Gurkan Erdogan, Beril Tulu, Hatice Nur Tarakcioglu
PURPOSE: To investigate the outcomes of subtotal vitrectomy in epiretinal membrane (ERM) and idiopathic macular hole (IMH) surgeries. METHODS: The patients who underwent vitrectomy for primary ERM and IMH were included. After the truncation of posterior hyaloid, cortical vitreous was incompletely removed and anterior vitreous was left in place. The main outcome measure was the complications of the surgical technique during the postoperative 12 months of follow-up...
February 7, 2018: International Ophthalmology
Merja R Häkkinen, Taija Heinosalo, Niina Saarinen, Tero Linnanen, Raimo Voutilainen, Timo Lakka, Jarmo Jääskeläinen, Matti Poutanen, Seppo Auriola
An LC-MS/MS method was developed and validated to analyze simultaneously estrogens (estradiol, E2; estrone, E1), androgens (testosterone, T; androstenedione, A4; dehydroepiandrosterone, DHEA), progestagens (17a-hydroxypregnenolone, 17OHP5; 17a-hydroxyprogesterone, 17OHP4; progesterone, P4), glucocorticoids (cortisol, F; cortisone E; corticosterone, B; 11-deoxycortisol, S; 21-hydroxyprogesterone, 21OHP4), and mineralocorticoids (aldosterone, A) from 150 μl of human serum, plasma, or endometrium and endometriotic tissue homogenates...
January 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
Adam Sajnóg, Anetta Hanć, Ryszard Koczorowski, Krzysztof Makuch, Danuta Barałkiewicz
Despite the fact that titanium is considered highly biocompatible, its presence in the oral cavity (an environment of frequently changing pH and temperature) may result in the release of titanium from intraosseous implants into the oral mucosa, causing a range of reactions from the human body. Fragments of oral mucosa collected from patients after dental implant insertion were analyzed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The study revealed an elevated content of elements (Ti, Al, V) which are components of the metal implants and temporary cover screws...
March 2018: Journal of Trace Elements in Medicine and Biology
J M Giambruni, C Pagano, T Ortiz Basso, A I Burchakchi, M C Pozzoni, D V Diamint, C F Challiol, G Gossn, B Boietti
OBJECTIVES: To determine whether patients with glaucoma and epiretinal membrane (ERM) use a greater proportion of prostaglandin analogues (PA) than a control group of patients with glaucoma without ERM. METHOD: A retrospective study of cases and controls was conducted in order to determine whether patients with glaucoma and ERM used a greater proportion of PA than a control group of patients with glaucoma without ERM. The diagnosis of de ERM was made by clinical examination and optical coherence tomography...
February 1, 2018: Archivos de la Sociedad Española de Oftalmología
Ilona P Foik, Irina Tuszynska, Marcin Feder, Elzbieta Purta, Filip Stefaniak, Janusz M Bujnicki
In erythromycin-resistant bacteria, the N6 position of A2058 in 23S rRNA is mono- or dimethylated by Erm family methyltransferases. This modification results in cross-resistance to macrolides, lincosamides and streptogramin B. Most inhibitors of Erm methyltransferases developed up-to-date target the cofactor-binding pocket, resulting in a lack of selectivity whereas inhibitors that bind the substrate-binding pocket demonstrate low in vitro activity. In this study, a molecular docking approach followed by biochemical screening was applied to search for inhibitors targeting both cofactor- and substrate-binding pockets of ErmC' methyltransferase...
January 6, 2018: European Journal of Medicinal Chemistry
Jiaqi Liao, Tao Hong, Jinxian Xu, Erming Zeng, Bin Tang, Wentao Lai
BACKGROUND AND PURPOSE: Moyamoya disease has a high incidence of cerebral vascular accident in children and adolescents, which can endanger the physical and mental health of children and adults seriously. However, the etiology and the pathogenesis of moyamoya disease remain unclear. Connexin43 (Cx43) is a predominant intercellular gap junction protein that plays an important role in the normal function of arteries and the development of several cardiovascular diseases. We aimed to preliminarily investigate pathological changes and the expression of Cx43 in cerebral arteries of patients with moyamoya disease...
January 25, 2018: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
Ilkay Kilic Muftuoglu, Tiezhu Lin, Eric Nudleman, Raouf Gaber, Dirk-Uwe Bartsch, William R Freeman
PURPOSE: To demonstrate factors related to the presence of subfoveal detachment (SD) secondary to epiretinal membrane (ERM) and to characterize the predictors for the resolution of SD after membrane peeling. METHODS: A retrospective chart review was conducted for the patients who underwent pars plana vitrectomy for the idiopathic ERM peeling. Preoperative spectral domain optical coherence tomography characteristics of the eyes including central foveal thickness, foveal center point thickness, presence of intraretinal cyst, continuity of the membrane, area of the membrane within fovea (1 mm), and entire macula (6 mm) were evaluated to find the best predictors for the presence of SD...
January 31, 2018: Retina
Daniele Tognetto, Chiara De Giacinto, Rossella D Apos Aloisio, Claudia Papagno, Marco Pastore, Marina Zweyer
PURPOSE: To report on the combined use of trypan blue (TB) and brilliant blue G (BBG) for staining the epiretinal membrane (ERM) and internal limiting membrane (ILM) during vitrectomy and to describe the histopathological findings. METHODS: 10 surgical specimens were removed from 10 eyes with macular pucker during vitrectomy using a commercially available combination of TB and BBG for ERM and ILM staining and peeling. Specimens were evaluated using light and transmission electron microscopy...
January 25, 2018: Ophthalmologica. Journal International D'ophtalmologie
Bing Bai, Kaitao Hu, Hui Li, Weiming Yao, Duoyun Li, Zhong Chen, Hang Cheng, Jinxin Zheng, Weiguang Pan, Minggui Deng, Xiaojun Liu, Zhiwei Lin, Qiwen Deng, Zhijian Yu
Enterococcal infections have become one of the most challenging nosocomial problems. Tedizolid, the second oxazolidinone is four- to eight-fold more potent in vivo and in vitro than linezolid against enterococci. However, the characteristics of tedizolid related to enterococci isolates in China remain elusive. The aim of this study was to evaluate in vitro activity of tedizolid against enterococcal isolates from patients with infections at a teaching hospital in China and to investigate the correlations between in vitro tedizolid activity against enterococci and the distribution of multilocus sequence types (MLST), resistance genes, and virulence factors...
December 27, 2017: FEMS Microbiology Letters
Sébastien Fumeron, Fernando Moraes, Erms Pereira
One interesting way to control heat is to use devices designed by transformation thermics, where artificial media are used. However, once manufactured (either repelling or concentrating heat, for example), besides being mono-purpose, such devices are designed according to a specific geometric boundary conditions. Another problem is the temperature dependence of the materials employed, since their properties are sometimes considered temperature-invariant. In this paper, we show that a previously proposed bi-objective heat switcher (Phys...
February 2, 2018: European Physical Journal. E, Soft Matter
Nanna B Svenningsen, Stephanie J Watts-Williams, Erik J Joner, Fabio Battini, Aikaterini Efthymiou, Carla Cruz-Paredes, Ole Nybroe, Iver Jakobsen
Arbuscular mycorrhizal fungi (AMF) colonise roots of most plants; their extra-radical mycelium (ERM) extends into the soil and acquires nutrients for the plant. The ERM coexists with soil microbial communities and it is unresolved whether these communities stimulate or suppress the ERM activity. This work studied the prevalence of suppressed ERM activity and identified main components behind the suppression. ERM activity was determined by quantifying ERM-mediated P uptake from radioisotope-labelled unsterile soil into plants, and compared to soil physicochemical characteristics and soil microbiome composition...
January 30, 2018: ISME Journal
Bryant C Roberts, Lucian B Solomon, Graham Mercer, Karen J Reynolds, Dominic Thewlis, Egon Perilli
OBJECTIVE: To study, in end-stage knee osteoarthritis (OA) patients, relationships between indices of in vivo dynamic knee joint loads obtained pre-operatively using gait analysis, static knee alignment, and the subchondral trabecular bone (STB) microarchitecture of their excised tibial plateau quantified with 3D micro-CT. DESIGN: Twenty-five knee OA patients scheduled for total knee arthroplasty underwent pre-operative gait analysis. Mechanical axis deviation (MAD) was determined radiographically...
January 27, 2018: Osteoarthritis and Cartilage
Xiaolin Wang, Xuelian Cao, Shunming Li, Qianting Ou, Dongxin Lin, Zhenjiang Yao, Sidong Chen, Chuan'an Wu, Guoming Wen, Xiaohua Ye
Streptococcus agalactiae (GBS) remains a major cause of invasive infections in neonates and pregnant women. Our aim was to evaluate the phenotypic and molecular characteristics of GBS isolates in order to reveal potential relationships among molecular characteristics and differences in genotype-phenotype characteristics between ST17 and ST19. A total of 104 GBS isolates were collected from pregnant women. All isolates were tested for antibiotic susceptibility by disk diffusion method and molecular characteristics, including antibiotic-resistant genes, virulence genes, serotypes and STs...
January 26, 2018: Research in Microbiology
Nobuhiko Shiraki, Susumu Sakimoto, Hirokazu Sakaguchi, Kentaro Nishida, Kohji Nishida, Motohiro Kamei
PURPOSE: To compare the anatomic and functional outcomes of pars plana vitrectomy (PPV) for treating rhegmatogenous retinal detachments (RRDs) between two groups with and without postoperative prone positioning. METHODS: This retrospective cohort study included 142 eyes of 142 patients with a primary RRD. All patients underwent PPV with 20% sulfur hexafluoride gas tamponade and were divided into two groups: the groups that did and did not maintain a prone position postoperatively...
2018: PloS One
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