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https://www.readbyqxmd.com/read/28103180/fluoroquinolone-macrolide-and-ketolide-resistance-in-haemophilus-parainfluenzae-from-south-africa
#1
Regina Esinam Abotsi, Usha Govinden, Krishnee Moodley, Sabiha Essack
Fluoroquinolones and ketolides are among the drugs of choice for the treatment of Haemophilus parainfluenzae infections. There has been a report of an emerging fluoroquinolone and telithromycin resistance in H. parainfluenzae isolates from the private sector of KwaZulu-Natal Province of South Africa that necessitates molecular investigation. The aim of this study is to characterize these resistance delineating mutations in genes commonly associated with reduced susceptibility. Ten H. parainfluenzae isolates retrieved from the sputum of 10 patients with H...
January 19, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28098723/assessment-of-the-internal-limiting-membrane-status-when-a-macular-epiretinal-membrane-is-removed-in-a-prospective-study
#2
Daniel Vila Solà, Cristofer Nienow, Ignasi Jürgens
PURPOSE: To describe the presence and integrity of the internal limiting membrane (ILM) after removal of the macular epiretinal membrane (ERM) and to investigate the accuracy of tissue identification using surgical dyes when compared to histopathology results. METHODS: Patients with idiopathic ERM were enrolled in a prospective study and randomized to one of two surgical techniques. In one (Group M), only the ERM was deliberately removed and in the other (Group L) the ILM was also removed...
January 16, 2017: Retina
https://www.readbyqxmd.com/read/28097770/bond-strength-and-reactivity-scales-for-lewis-superacid-adducts-a-comparative-study-with-in-otf-3-and-al-otf-3
#3
Guillaume Compain Compain, Lauri Sikk, Lionel Massi, Jean-François Gal, Elisabet Duñach
Metal triflates, often called Lewis superacids, are potent catalysts for organic synthesis. However, the reactivity of a given Lewis superacid toward a given base is difficult to anticipate. A systematic screening of catalysts is often necessary when developing synthetic methodologies. Presented herein is the development of quantitative reactivity and bond strength scales by using mass spectrometry (MS). By applying collision induced dissociation (CID) technique to the adducts formed between Lewis superacids Al(OTf)3 or In(OTf)3 with a series of amides bases, including monodentate and bidentate ligands, different dissociation pathways were observed...
January 18, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28096551/the-natural-evolution-of-idiophatic-epimacular-membrane
#4
Libor Hejsek, Alexandr Stepanov, Alena Dohnalova, Tereza Rehakova, Nada Jiraskova
BACKGROUND AND AIMS: Epiretinal membrane (ERM) refers to a semi-translucent tissue layer found on the inner surface of the retina especially in older people. Surgical treatment remains controversial, optimal timing for such treatment is difficult to determine and data on the natural evolution of this disorder are limited. In this study we evaluated the natural course of idiopathic epimacular membrane in 49 patients (53 eyes). MATERIALS AND METHODS: Retrospective evaluation of a group of 49 patients (53 eyes) with idiopathic epimacular membrane confirmed by biomicroscopy, photography and optical coherence tomography (OCT)...
January 13, 2017: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/28089219/prognostic-factors-of-epiretinal-membranes-a-systematic-review
#5
REVIEW
A I M Miguel, A Legris
INTRODUCTION: Epiretinal membranes (ERM) have been increasingly characterized with the advent of new optical coherence tomographies (OCTs). We intended to perform a systematic review regarding prognostic factors (PF) of ERM after surgery. MATERIALS AND METHODS: Systematic review of electronic databases was performed (last date of search was 10 August 2015): Medline, Scopus, Google Scholar, clinicaltrials.gov and current controlled trials. Search queries included: "membrane", "pucker", "prognosis", "prognostic", "epiretinal", "épirétinienne"...
January 11, 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/28076563/influence-of-the-epiretinal-membrane-on-ranibizumab-therapy-outcomes-in-patients-with-diabetic-macular-edema
#6
Nimet Yesim Ercalik, Serhat Imamoglu, Esra Turkseven Kumral, Nursal Melda Yenerel, Handan Bardak, Yavuz Bardak
Purpose: To investigate the influence of the epiretinal membrane (ERM) on intravitreal ranibizumab (IVR) therapy for diabetic macular edema (DME). Methods: This retrospective study included 56 eyes of 48 patients with DME divided into two groups: the DME with ERM (study) and only DME (control) groups. Changes in the central macular thickness (CMT) and best-corrected visual acuity (BCVA) were evaluated. Results: In the study group, although the CMT was significantly reduced following the first injection (p<0...
November 2016: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28074049/in-vitro-antibacterial-activity-of-%C3%AE-methoxyimino-acylide-derivatives-against-macrolide-resistant-pathogens-and-mutation-analysis-in-23s-rrna
#7
Hiroyuki Sugiyama, Ippei Yoshida, Mayumi Ueki, Katsuhiko Tanabe, Akira Manaka, Keiichi Hiramatsu
We characterized in vitro activities of α-methoxyimino acylides against macrolide-resistant clinical isolates of Streptococcus pneumoniae, Streptococcus pyogenes and Mycoplasma pneumoniae with ribosome modification or substitution and selected acylide-resistant mutants to clarify the binding point of the acylides. The acylides had low MICs against erm(B) gene-containing S. pneumoniae and S. pyogenes (MIC90s, 1-4 μg ml(-1)). For M. pneumoniae, although they had poor potencies against macrolide-resistant strains with the A2058G (Escherichia coli numbering) mutation in 23S rRNA (MICs, >32 μg ml(-1)), one of them showed in vitro activities against macrolide-resistant strains with the A2058U or A2059G mutations (MICs, 0...
January 11, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28073902/state-of-harmonization-of-24-serum-albumin-measurement-procedures-and-implications-for-medical-decisions
#8
Lorin M Bachmann, Min Yu, James C Boyd, David E Bruns, W Greg Miller
BACKGROUND: Measurements of serum and plasma albumin are widely used in medicine, including as indicators of quality of patient care in renal dialysis centers. METHODS: Pools were prepared from residual patient serum (n = 50) and heparin plasma (n = 48) from patients without renal disease, and serum from patients with kidney failure before hemodialysis (n = 53). Albumin was measured in all samples and in ERM-DA470k/IFCC reference material (RM) by 3 immunochemical, 9 bromcresol green (BCG), and 12 bromcresol purple (BCP) methods...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28067350/enzyme-responsive-reporter-molecules-for-selective-localization-and-fluorescence-imaging-of-pathogenic-biofilms
#9
Junxin Aw, Frances Widjaja, Yichen Ding, Jing Mu, Yang Liang, Bengang Xing
Pathogenic bacteria and their biofilm formation are responsible for a broad spectrum of microbial infections. A novel enzyme-responsive reporter molecule (ERM-1), which can specifically recognize AmpC β-lactamase (Bla) in drug resistant bacteria, has been developed to enable the selective localization of biofilms.
January 9, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28059785/amylin-enhances-amyloid-%C3%AE-peptide-brain-to-blood-efflux-across-the-blood-brain-barrier
#10
Loqman A Mohamed, Haihao Zhu, Youssef M Mousa, Erming Wang, Wei Qiao Qiu, Amal Kaddoumi
Findings from Alzheimer's disease (AD) mouse models showed that amylin treatment improved AD pathology and enhanced amyloid-β (Aβ) brain to blood clearance; however, the mechanism was not investigated. Using the Tg2576 AD mouse model, a single intraperitoneal injection of amylin significantly increased Aβ serum levels, and the effect was abolished by AC253, an amylin receptor antagonist, suggesting that amylin effect could be mediated by its receptor. Subsequent mechanistic studies showed amylin enhanced Aβ transport across a cell-based model of the blood-brain barrier (BBB), an effect that was abolished when the amylin receptor was inhibited by two amylin antagonists and by siRNA knockdown of amylin receptor Ramp3...
December 3, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28056219/antimicrobial-resistant-escherichia-coli-and-enterococcus-spp-isolated-from-miranda-donkey-equus-asinus-an-old-problem-from-a-new-source-with-a-different-approach
#11
Isabel Carvalho, Rosa Del Campo, Margarida Sousa, Nuno Silva, João Carrola, Catarina Marinho, Tiago Santos, Silvia Carvalho, Miguel Nóvoa, Miguel Quaresma, José Eduardo Pereira, Marta Cobo, Gilberto Igrejas, Patricia Poeta
The Miranda Donkey (Equus asinus) is an endangered asinine from Miranda do Douro region, located in North East of Portugal. We study the antimicrobial resistance (AMR) and virulence genes in Escherichia coli and Enterococcus spp. isolates from these animals. In March 2014, a total of 66 faecal samples were recovered from independent animals. Antibiotic resistance was determined by the disk diffusion method. Carriage of genes codifying for antibiotic resistant and virulent factors was analyzed by polymerase chain reaction (PCR)...
January 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28045374/the-role-of-internal-limiting-membrane-peeling-in-epiretinal-membrane-surgery-a-randomised-controlled-trial
#12
Paris Tranos, Stavrenia Koukoula, Davic G Charteris, Georgia Perganda, Athanasios Vakalis, Solon Asteriadis, Ilias Georgalas, Petros Petrou
PURPOSE: To compare the anatomical and functional outcomes after primary idiopathic epiretinal membrane (ERM) peeling with or without internal limiting membrane (ILM) peeling. DESIGN: A two-centre randomised, controlled clinical trial with 12 months of follow-up. METHODS: One hundred and two eyes of 102 patients were included in the analysis and were randomised into two groups (ILM peeling (P) and non-ILM peeling (NP) group). Inclusion criteria were: Idiopathic ERM confirmed on optical coherence tomography, age ≥18 years, binocular distortion, best-corrected visual acuity (BCVA) ≤90 ETDRS letters, intraocular pressure ≤23 mm Hg and informed consent...
October 7, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28043309/visual-and-anatomical-outcomes-following-idiopathic-macular-epiretinal-membrane-surgery
#13
Bashiran Shahzadi, Syed Fawad Rizvi, Kanwal Latif, Faisal Murtaza, Saliha Naz
OBJECTIVE: To assess the visual and anatomical outcomes following idiopathic macular epiretinal membrane (IERM) surgery. STUDY DESIGN: Case series. PLACE AND DURATION OF STUDY: Layton Rehmatulla Benevolent Trust (L.R.B.T), Free Base Eye Hospital, Karachi, from January 2015 to June 2016. METHODOLOGY: Thirty eyes of thirty patients affected with idiopathic macular epiretinal membrane stage 2 were enrolled in this study...
December 2016: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28040223/congener-profiles-distribution-sources-and-ecological-risk-of-parent-and-alkyl-pahs-in-surface-sediments-of-southern-yellow-sea-china
#14
Yanxia Lin, Wei Deng, Shengyong Li, Jiafu Li, Guoguang Wang, Dahai Zhang, Xianguo Li
Congener profiles, distribution and sources of parent and alkyl-polycyclic aromatic hydrocarbons (PAHs) in surface sediments of Southern Yellow Sea (SYS), China were investigated. The ecological risk of parent-PAHs to aquatic organisms were evaluated based on sediment quality guidelines (SQGs), risk quotient (RQ), organic carbon (OC)-normalized analysis and mean effects range-median quotient (M-ERM-Q). The concentrations of 33 PAHs were analyzed, including 14 parent PAHs (without naphthalene and acenaphthylene), 16 alkyl-PAHs (7 methylphenanthrenes, 3 methylanthracenes, 3 methylfluoranthenes, and 3 methylpyrenes), dibenzothiophene, retene and perylene...
December 28, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/28011608/mycobacterial-characteristics-and-treatment-outcomes-in-mycobacterium-abscessus-lung-disease
#15
Won-Jung Koh, Byeong-Ho Jeong, Su-Young Kim, Kyeongman Jeon, Kyoung Un Park, Byung Woo Jhun, Hyun Lee, Hye Yun Park, Dae Hun Kim, Hee Jae Huh, Chang-Seok Ki, Nam Yong Lee, Hong Kwan Kim, Yong Soo Choi, Jhingook Kim, Seung-Heon Lee, Chang Ki Kim, Sung Jae Shin, Charles L Daley, Hojoong Kim, O Jung Kwon
BACKGROUND: Treatment outcomes of patients with Mycobacterium abscessus subspecies abscessus lung disease are poor, and the microbial characteristics associated with treatment outcomes have not been studied systematically. The purpose of this study was to identify associations between microbial characteristics and treatment outcomes in patients with M. abscessus lung disease. METHODS: Sixty-seven consecutive patients with M. abscessus lung disease undergoing antibiotic treatment for ≥12 months between January 2002 and December 2012 were included...
February 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28009345/the-role-of-membrane-inner-retina-adherence-in-predicting-simultaneous-internal-limiting-membrane-peeling-during-idiopathic-epiretinal-membrane-surgery
#16
P Tranos, L Wickham, N Dervenis, A Vakalis, S Asteriades, P Stavrakas
PurposeTo correlate the frequency and extent of simultaneous inadvertent internal limiting membrane (ILM) peeling during idiopathic epiretinal membrane (ERM) removal with characteristics of ERM adherence demonstrated on pre-operative spectral domain optical coherence tomography (SD-OCT).Patients and methodsThis is a prospective, observational, case series of patients undergoing pars plana vitrectomy for idiopathic ERM. Inner retina-ERM adhesion was categorized as focal, broad or complete in five anatomic locations at macular area based on preoperative SD-OCT findings...
December 23, 2016: Eye
https://www.readbyqxmd.com/read/28003600/identification-of-group-g-streptococci-isolates-from-companion-animals-in-japan-and-their-antimicrobial-resistance
#17
Yuzo Tsuyuki, Goro Kurita, Yoshiteru Murata, Mieko Goto, Takashi Takahashi
This study determined species-level identification and antimicrobial resistance (AMR) properties in group G streptococci (GGS) isolates from companion animals in Japan. Strains were isolated from sterile/non-sterile specimens of 72 animals with clinical symptoms or signs in April-May, 2015. We identified them based on 16S rRNA sequencing, mass spectrometry (MS), and an automated method based on their biochemical properties. Antimicrobial susceptibility was determined with broth microdilution method and Etest...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28002284/outcomes-of-anti-vegf-therapy-in-choroidal-neovascularization-after-macular-surgery
#18
Duc Anh Hua, Antonio M Casella, Maria H Berrocal, Dennis Han, Rayan A Alshareef, Hugo Quiroz-Mercado, J Fernando Arevalo, Jay Chhablani
PURPOSE: To report treatment outcomes of anti-vascular endothelial growth factor (VEGF) therapy in choroidal neovascularization (CNV) presenting after macular surgery. METHODS: Retrospective analysis of 7 eyes of 7 patients, who were diagnosed to have CNV after macular surgery and were treated with anti-vascular endothelial growth factor therapy. Collected data included demographic details; history of present illness; surgical procedure; and clinical examination including visual acuity at presentation and follow-up with imaging and management...
December 20, 2016: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/27997034/changes-in-turnover-rather-than-production-regulate-biomass-of-ectomycorrhizal-fungal-mycelium-across-a-pinus-sylvestris-chronosequence
#19
Andreas Hagenbo, Karina E Clemmensen, Roger D Finlay, Julia Kyaschenko, Björn D Lindahl, Petra Fransson, Alf Ekblad
In boreal forest soils, ectomycorrhizal fungi are fundamentally important for carbon (C) dynamics and nutrient cycling. Although their extraradical mycelium (ERM) is pivotal for processes such as soil organic matter build-up and nitrogen cycling, very little is known about its dynamics and regulation. In this study, we quantified ERM production and turnover, and examined how these two processes together regulated standing ERM biomass in seven sites forming a chronosequence of 12- to 100-yr-old managed Pinus sylvestris forests...
December 20, 2016: New Phytologist
https://www.readbyqxmd.com/read/27993592/insights-into-epiretinal-membranes-presence-of-ectopic-inner-foveal-layers-and-a-new-optical-coherence-tomography-staging-scheme
#20
Andrea Govetto, Robert A Lalane, David Sarraf, Marta S Figueroa, Jean Pierre Hubschman
PURPOSE: To describe the presence of continuous ectopic inner foveal layers associated with epiretinal membranes (ERMs) and to present a new optical coherence tomography (OCT) staging system of ERMs. DESIGN: Retrospective multicenter observational case series. METHODS: Clinical charts and Spectral-Domain OCT images of 194 eyes of 172 consecutive patients diagnosed with ERMs were reviewed and analyzed. RESULTS: The presence of continuous ectopic inner foveal layers was identified in 63 out of 194 eyes (32...
December 16, 2016: American Journal of Ophthalmology
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