keyword
MENU ▼
Read by QxMD icon Read
search

Mitotic gene

keyword
https://www.readbyqxmd.com/read/28434146/differential-regulation-of-h3s10-phosphorylation-mitosis-progression-and-cell-fate-by-aurora-kinase-b-and-c-in-mouse-preimplantation-embryos
#1
Wenzhi Li, Peizhe Wang, Bingjie Zhang, Jing Zhang, Jia Ming, Wei Xie, Jie Na
Coordination of cell division and cell fate is crucial for the successful development of mammalian early embryos. Aurora kinases are evolutionarily conserved serine/threonine kinases and key regulators of mitosis. Aurora kinase B (AurkB) is ubiquitously expressed while Aurora kinase C (AurkC) is specifically expressed in gametes and preimplantation embryos. We found that increasing AurkC level in one blastomere of the 2-cell embryo accelerated cell division and decreasing AurkC level slowed down mitosis. Changing AurkB level had the opposite effect...
April 22, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28431155/motoring-through-the-role-of-kinesin-superfamily-proteins-in-female-meiosis
#2
Nicole J Camlin, Eileen A McLaughlin, Janet E Holt
BACKGROUND: The kinesin motor protein family consists of 14 distinct subclasses and 45 kinesin proteins in humans. A large number of these proteins, or their orthologues, have been shown to possess essential function(s) in both the mitotic and the meiotic cell cycle. Kinesins have important roles in chromosome separation, microtubule dynamics, spindle formation, cytokinesis and cell cycle progression. This article contains a review of the literature with respect to the role of kinesin motor proteins in female meiosis in model species...
April 18, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28429277/primary-ewing-sarcoma-primitive-neuroectodermal-tumor-of-the-kidney-a-clinicopathologic-study-of-23-cases
#3
Paari Murugan, Priya Rao, Pheroze Tamboli, Bogdan Czerniak, Charles C Guo
Primary Ewing sarcoma / primitive neuroectodermal tumor (ES) of the kidney is a rare neoplasm with limited clinicopathologic data. We report 23 such cases with no history of ES elsewhere in the body. The patients included 13 male and 10 female, aged 8-70 years (mean, 31 years). The average tumor size was 11.7 cm (range, 5-20 cm). Microscopic analysis showed predominantly lobular growth (n = 14), with focal papillary (n = 3), alveolar (n = 1), and hemangiopericytoma-like (n = 1) patterns. Several tumors (n = 11) exhibited robust mitotic activity (>10 mitoses/10 high-power fields)...
April 20, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28428263/different-requirements-of-functional-telomeres-in-neural-stem-cells-and-terminally-differentiated-neurons
#4
Anastasia Lobanova, Robert She, Simon Pieraut, Charlie Clapp, Anton Maximov, Eros Lazzerini Denchi
Telomeres have been studied extensively in peripheral tissues, but their relevance in the nervous system remains poorly understood. Here, we examine the roles of telomeres at distinct stages of murine brain development by using lineage-specific genetic ablation of TRF2, an essential component of the shelterin complex that protects chromosome ends from the DNA damage response machinery. We found that functional telomeres are required for embryonic and adult neurogenesis, but their uncapping has surprisingly no detectable consequences on terminally differentiated neurons...
April 20, 2017: Genes & Development
https://www.readbyqxmd.com/read/28426272/temperature-dependent-regulation-of-rdna-condensation-in-saccharomyces-cerevisiae
#5
Donglai Shen, Robert V Skibbens
Chromatin condensation during mitosis produces detangled and discrete DNA entities required for high fidelity sister chromatid segregation during mitosis and positions DNA away from the cleavage furrow during cytokinesis. Regional condensation during G1 also establishes a nuclear architecture through which gene transcription is regulated but remains plastic so that cells can respond to changes in nutrient levels, temperature and signaling molecules. To date, however, the potential impact of this plasticity on mitotic chromosome condensation remains unknown...
April 20, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28424234/braf-v600-mutation-detection-in-melanoma-a-comparison-of-two-laboratory-testing-methods
#6
Odharnaith O'Brien, Tomas Lyons, Sandra Murphy, Linda Feeley, Derek Power, Cynthia C B B Heffron
AIMS: The assessment of B-raf proto-oncogene, serine/threonine kinase (BRAF) gene status is now standard practice in patients diagnosed with metastatic melanoma with its presence predicting a clinical response to treatment with BRAF inhibitors. The gold standard in determining BRAF status is currently by DNA-based methods. More recently, a BRAF V600E antibody has been developed. We aim to investigate whether immunohistochemical detection of BRAF mutation is a suitable alternative to molecular testing by polymerase chain reaction (PCR)...
April 19, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28423514/mitotic-read-out-genes-confer-poor-outcome-in-luminal-a-breast-cancer-tumors
#7
Javier Pérez-Peña, Ana Alcaraz-Sanabria, Cristina Nieto-Jiménez, Raquel Páez, Verónica Corrales-Sánchez, Leticia Serrano-Oviedo, Vikram B Wali, Gauri A Patwardhan, Eitan Amir, Balázs Győrffy, Atanasio Pandiella, Alberto Ocaña
Luminal breast tumors have been classified into A and B subgroups, with the luminal A being associated with a more favorable clinical outcome. Unfortunately, luminal A tumors do not have a universally good prognosis. We used transcriptomic analyses using public datasets to evaluate the differential expression between normal breast tissue and breast cancer, focusing on upregulated genes included in cell cycle function. Association of selected genes with relapse free survival (RFS) and overall survival (OS) was performed using the KM Plotter Online Tool (http://www...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423198/an-estradiol-inducible-promoter-enables-fast-graduated-control-of-gene-expression-in-fission-yeast
#8
Makoto J Ohira, David G Hendrickson, R Scott McIsaac, Nicholas Rhind
The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human estrogen receptor, for use in S...
April 19, 2017: Yeast
https://www.readbyqxmd.com/read/28416637/a-kinase-independent-role-for-cdk19-in-p53-response
#9
K Audrey Audetat, Matthew D Galbraith, Aaron T Odell, Thomas Lee, Ahwan Pandey, Joaquin M Espinosa, Robin D Dowell, Dylan J Taatjes
The human Mediator complex regulates RNA Polymerase II transcription genome-wide. A general factor that regulates Mediator function is the four-subunit kinase module, which contains either CDK8 or CDK19. Whereas CDK8 is linked to specific signaling cascades and oncogenesis, the cellular roles of its paralog, CDK19, are poorly studied. We discovered that osteosarcoma cells (SJSA) are naturally depleted of CDK8 protein. Whereas stable CDK19 knockdown was tolerated in SJSA cells, proliferation was reduced. Notably, proliferation defects were rescued upon re-expression of wild-type or kinase-dead CDK19...
April 17, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28416635/vprbp-dcaf1-regulates-the-degradation-and-non-proteolytic-activation-of-the-cell-cycle-transcription-factor-foxm1
#10
Xianxi Wang, Anthony Arceci, Kelly Bird, Christine A Mills, Rajarshi Choudhury, Jennifer L Kernan, Chunxiao Zhou, Victoria Bae-Jump, Albert Bowers, Michael J Emanuele
The oncogenic transcription factor FoxM1 plays a vital role in cell cycle progression, is activated in numerous human malignancies and linked to chromosome instability. We characterize here a Cullin4-based E3 ubiquitin ligase and its substrate receptor VprBP/DCAF1 (CRL4(VprBP)) that we show regulate FoxM1 ubiquitylation and degradation. Paradoxically, we also found that the substrate receptor VprBP is a potent FoxM1 activator. VprBP depletion reduces expression of FoxM1 target genes and impairs mitotic entry, whereas ectopic VprBP expression strongly activates a FoxM1 transcriptional reporter...
April 17, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28407751/transcriptome-analysis-reveals-potential-mechanisms-underlying-differential-heart-development-in-fast-and-slow-growing-broilers-under-heat-stress
#11
Jibin Zhang, Carl J Schmidt, Susan J Lamont
BACKGROUND: Modern fast-growing broilers are susceptible to heart failure under heat stress because their relatively small hearts cannot meet increased need of blood pumping. To improve the cardiac tolerance to heat stress in modern broilers through breeding, we need to find the important genes and pathways that contribute to imbalanced cardiac development and frequent occurrence of heat-related heart dysfunction. Two broiler lines - Ross 708 and Illinois - were included in this study as a fast-growing model and a slow-growing model respectively...
April 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28404951/search-for-rare-protein-altering-variants-influencing-susceptibility-to-multiple-myeloma
#12
Matthew Scales, Daniel Chubb, Sara E Dobbins, David C Johnson, Ni Li, Michael J Sternberg, Neils Weinhold, Caleb Stein, Graham Jackson, Faith E Davies, Brian A Walker, Christopher P Wardell, Richard S Houlston, Gareth J Morgan
The genetic basis underlying the inherited risk of developing multiple myeloma (MM) is largely unknown. To examine the impact of rare protein altering variants on the risk of developing MM we analyzed high-coverage exome sequencing data on 513 MM cases and 1,569 healthy controls, performing both single variant and gene burden tests. We did not identify any recurrent coding low-frequency alleles (1-5%) with moderate effect that were statistically associated with MM. In a gene burden analysis we did however identify a promising relationship between variation in the marrow kinetochore microtubule stromal gene KIF18A, which plays a role in control mitotic chromosome positioning dynamics, and risk of MM (P =3...
March 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28404741/avian-blastoderm-dormancy-arrests-cells-in-g2-and-suppresses-apoptosis
#13
Mee Hyun Ko, Young Sun Hwang, Jong Seop Rim, Ho Jae Han, Jae Yong Han
In most avian species, the early embryo suspends development when the ambient temperature is too low; the resultant dormant state is called cold torpor. However, very little is known about dormant avian embryos at the cellular level. To investigate the molecular processes that occur in the chicken blastoderm during cold torpor, we performed transcriptome analysis and investigated cellular responses in dormant embryos. In embryos stored at low temperature, we observed up-regulation of genes and proteins related to endoplasmic reticulum stress and stress-activated protein kinase signaling...
April 12, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28400510/malate-dependent-fe-accumulation-is-a-critical-checkpoint-in-the-root-developmental-response-to-low-phosphate
#14
Javier Mora-Macías, Jonathan Odilón Ojeda-Rivera, Dolores Gutiérrez-Alanís, Lenin Yong-Villalobos, Araceli Oropeza-Aburto, Javier Raya-González, Gabriel Jiménez-Domínguez, Gabriela Chávez-Calvillo, Rubén Rellán-Álvarez, Luis Herrera-Estrella
Low phosphate (Pi) availability constrains plant development and seed production in both natural and agricultural ecosystems. When Pi is scarce, modifications of root system architecture (RSA) enhance the soil exploration ability of the plant and lead to an increase in Pi uptake. In Arabidopsis, an iron-dependent mechanism reprograms primary root growth in response to low Pi availability. This program is activated upon contact of the root tip with low-Pi media and induces premature cell differentiation and the arrest of mitotic activity in the root apical meristem, resulting in a short-root phenotype...
April 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28398645/crispr-cas9-mediated-gene-knockout-in-the-mouse-brain-using-in-utero-electroporation
#15
Yohei Shinmyo, Hiroshi Kawasaki
This unit describes a highly efficient and rapid procedure for brain-specific disruption of genes in the developing mouse brain using pX330 plasmids expressing humanized Cas9 and single-guide RNAs (sgRNAs) against target genes. The pX330 plasmids are delivered into the rodent brain using in utero electroporation. Focusing on the Satb2 gene, which encodes an AT-rich DNA-binding transcription factor, we found that the introduction of pX330-Satb2 induced insertion/deletion (indel) mutations near the predicted cleavage site in the Satb2 gene, resulting in a dramatic reduction of Satb2 expression in post-mitotic neurons...
April 10, 2017: Current Protocols in Neuroscience
https://www.readbyqxmd.com/read/28396626/pattern-of-neurogenesis-and-identification-of-neuronal-progenitor-subtypes-during-pallial-development-in-xenopus-laevis
#16
Nerea Moreno, Agustín González
The complexity of the pallium during evolution has increased dramatically in many different respects. The highest level of complexity is found in mammals, where most of the pallium (cortex) shows a layered organization and neurons are generated during development following an inside-out order, a sequence not observed in other amniotes (birds and reptiles). Species-differences may be related to major neurogenetic events, from the neural progenitors that divide and produce all pallial cells. In mammals, two main types of precursors have been described, primary precursor cells in the ventricular zone (vz; also called radial glial cells or apical progenitors) and secondary precursor cells (called basal or intermediate progenitors) separated from the ventricle surface...
2017: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/28386673/development-of-an-agrobacterium-mediated-transformation-method-and-evaluation-of-two-exogenous-constitutive-promoters-in-oleaginous-yeast-lipomyces-starkeyi
#17
Xinping Lin, Sasa Liu, Ruiqi Bao, Ning Gao, Sufang Zhang, Rongqian Zhu, Zongbao Kent Zhao
Oleaginous yeast Lipomyces starkeyi, a promising strain of great biotechnical importance, is able to accumulate over 60% of its cell biomass as triacylglycerols (TAGs). It is promising to directly produce the derivatives of TAGs, such as long-chain fatty acid methyl esters and alkanes, in L. starkeyi. However, techniques for genetic modification of this oleaginous yeast are lacking, thus, further research is needed to develop genetic tools and functional elements. Here, we used two exogenous promoters (pGPD and pPGK) from oleaginous yeast Rhodosporidium toruloides to establish a simpler Agrobacterium-mediated transformation (AMT) method for L...
April 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28381640/early-b-cell-progenitors-deficient-for-gon4l-fail-to-differentiate-due-to-a-block-in-mitotic-cell-division
#18
Jennifer Y Barr, Renee X Goodfellow, Diana F Colgan, John D Colgan
B cell development in Justy mutant mice is blocked due to a precursor mRNA splicing defect that depletes the protein GON4-like (GON4L) in B cell progenitors. Genetic and biochemical studies have suggested that GON4L is a transcriptional regulator that coordinates cell division with differentiation, but its role in B cell development is unknown. To understand the function of GON4L, we characterized B cell differentiation, cell cycle control, and mitotic gene expression in GON4L-deficient B cell progenitors from Justy mice...
April 5, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28381587/properties-of-mitotic-and-meiotic-recombination-in-the-tandemly-repeated-cup1-gene-cluster-in-the-yeast-saccharomyces-cerevisiae
#19
Ying Zhao, Margaret Dominska, Aleksandra Petrova, Halle Bagshaw, Robert J Kokoska, Thomas D Petes
In the yeast Saccharomyces cerevisiae, the genes encoding the metallothionein protein Cup1 are located in a tandem array on chromosome VIII. Using a diploid strain that is heterozygous for an insertion of a selectable marker (URA3) within this tandem array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold...
April 4, 2017: Genetics
https://www.readbyqxmd.com/read/28380054/meioc-maintains-an-extended-meiotic-prophase-i-in-mice
#20
Y Q Shirleen Soh, Maria M Mikedis, Mina Kojima, Alexander K Godfrey, Dirk G de Rooij, David C Page
The meiosis-specific chromosomal events of homolog pairing, synapsis, and recombination occur over an extended meiotic prophase I that is many times longer than prophase of mitosis. Here we show that, in mice, maintenance of an extended meiotic prophase I requires the gene Meioc, a germ-cell specific factor conserved in most metazoans. In mice, Meioc is expressed in male and female germ cells upon initiation of and throughout meiotic prophase I. Mouse germ cells lacking Meioc initiate meiosis: they undergo pre-meiotic DNA replication, they express proteins involved in synapsis and recombination, and a subset of cells progress as far as the zygotene stage of prophase I...
April 2017: PLoS Genetics
keyword
keyword
79240
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"