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Enzymatic hydrolysis

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https://www.readbyqxmd.com/read/28723955/crystal-structure-and-functional-characterization-of-an-isoaspartyl-dipeptidase-cpsiada-from-colwellia-psychrerythraea-strain-34h
#1
Sun-Ha Park, Chang Woo Lee, Sung Gu Lee, Seung Chul Shin, Hak Jun Kim, Hyun Park, Jun Hyuck Lee
Isoaspartyl dipeptidase (IadA) is an enzyme that catalyzes the hydrolysis of an isoaspartyl dipeptide-like moiety, which can be inappropriately formed in proteins, between the β-carboxyl group side chain of Asp and the amino group of the following amino acid. Here, we have determined the structures of an isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea, both ligand-free and that complexed with β-isoaspartyl lysine, at 1.85-Å and 2.33-Å resolution, respectively. In both structures, CpsIadA formed an octamer with two Zn ions in the active site...
2017: PloS One
https://www.readbyqxmd.com/read/28721652/effects-of-liquid-hot-water-pretreatment-on-enzymatic-hydrolysis-and-physicochemical-changes-of-corncobs
#2
Saksit Imman, Navadol Laosiripojana, Verawat Champreda
Liquid hot water (LHW) pretreatment is an efficient chemical-free strategy for enhancing enzymatic digestibility of lignocellulosic biomass for conversion to fuels and chemicals in biorefinery. In this study, effects of LHW on removals of hemicelluloses and lignin from corncobs were studied under varying reaction conditions. LHW pretreatment at 160 °C for 10 min promoted the highest levels of hemicellulose solubilization into the liquid phase, resulting into the maximized pentose yield of 58.8% in the liquid and more than 60% removal of lignin from the solid, with 73...
July 19, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28718847/effect-of-pretreatment-severity-on-the-cellulose-and-lignin-isolated-from-salix-using-ionosolv-pretreatment
#3
Lisa Weigand, Shahrokh Mostame, Agnieszka Brandt-Talbot, Tom Welton, Jason P Hallett
The ionoSolv pretreatment is a new technique employing protic low-cost ionic liquids and has previously been applied to successfully fractionate switchgrass and the grass Miscanthus giganteus. This study investigates the effect of using the protic ionic liquid solution [N2220][HSO4]80% with two different acid/base ratios (1.02 and 0.98) at 120, 150 and 170 °C on the pretreatment outcome of the hardwood willow. The ionic liquid solution was able to fractionate willow, and a pulp and lignin fraction were recovered after treatment...
July 18, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28716292/production-of-sodium-gluconate-from-delignified-corn-cob-residue-by-on-site-produced-cellulase-and-co-immobilized-glucose-oxidase-and-catalase
#4
Xiaolong Han, Guodong Liu, Wenxia Song, Yinbo Qu
The production of sodium gluconate by enzymatic catalysis of delignified corn cob residue (DCCR) hydrolysate was studied. Penicillium oxalicum I1-13 was used for the production of cellulase with high β-glucosidase activity. A fed-batch saccharification process was developed to obtain high yields of glucose. At the end of hydrolysis, the concentration of glucose reached 145.80g/L. Glucose oxidase and catalase were co-immobilized to catalyze DCCR hydrolysate to produce sodium gluconate. Under the optimum conditions, 166...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28715790/site-and-species-specific-hydrolysis-rates-of-cocaine
#5
Levente Szöcs, Gergely Völgyi, Ákos Urai, Sándor Hosztafi, Gergő Tóth, Lajos Gergó, Béla Noszál
The hydroxide-catalyzed non-enzymatic hydrolysis of cocaine is quantified in terms of ten site- and species-specific rate constants in connection with also ten site- and species-specific acid-base equilibrium constants, comprising all the twelve coexisting species in solution. This characterization involves the major and minor decomposition pathways via benzoylecgonine and ecgonine methyl ester, respectively, leading to ecgonine, the final product. Hydrolysis has been found to be 10-330 times faster at site 2 than at site 3, depending on the ionization status of the amino moiety and the rest of the molecule...
July 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28714138/production-of-%C3%AE-lactoglobulin-hydrolysates-by-monolith-based-immobilized-trypsin-reactors
#6
Yuhong Mao, Urh Černigoj, Viktor Zalokar, Aleš Štrancar, Ulrich Kulozik
Tryptic hydrolysis of β-Lactoglobulin (β-Lg) is attracting more and more attention due to the reduced allergenicity and the functionality of resulting hydrolysates. To produce hydrolysates in an economically viable way, immobilized trypsin reactors (IMTRs), based on polymethacrylate monolith with pore size 2.1 μm (N1) and 6 μm (N2), were developed and used in a flow-through system. IMTRs were characterized in terms of permeability and enzymatic activity during extensive usage. N1 showed twice the activity compared with N2, correlating well with its almost two times higher amount of immobilized trypsin...
July 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28711616/isolation-screening-and-characterization-of-a-novel-extracellular-xylanase-from-aspergillus-niger-kp874102-1-and-its-application-in-orange-peel-hydrolysis
#7
Uma Shankar Prasad Uday, Ria Majumdar, Onkar Nath Tiwari, Umesh Mishra, Abhijit Mondal, Tarun Kanti Bandyopadhyay, Biswanath Bhunia
In the present work, a potent xylanase producing fungal strain Aspergillus niger (KP874102.1) was isolated through cultural and morphological observations from soil sample of Baramura forest, Tripura west, India. 28S rDNA technique was applied for genomic identification of this fungal strain. The isolated strain was found to be phylogenetically closely related to Aspergillus niger. Kinetic constants such as Km and Vmax for extracellular xylanase were determined using various substrate such as beech wood xylan, oat spelt xylan and CM cellulose through Lineweaver-Burk plot...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28710970/validation-of-a-probe-for-assessing-deconjugation-of-glucuronide-and-sulfate-phase-ii-metabolites-assayed-through-lc-ms-ms-in-biological-matrices
#8
Claire Grignon, Antoine Dupuis, Marion Albouy-Llaty, Maxime Condylis, Laurence Barrier, Pascal Carato, Bertrand Brunet, Virginie Migeot, Nicolas Venisse
LC-MS/MS has been proposed in various areas such as Therapeutic Drug Monitoring (TDM), Human Biomonitoring (HBM), disease diagnosis, clinical toxicology and doping control to identify and quantify chemical parents and their metabolites in biological matrices. To determine the total content of a xenobiotic (unconjugated+conjugated forms), an enzymatic hydrolysis step is required. Most studies in the literature have not controlled the effectiveness of the deconjugation process because no method has been described for that purpose...
July 10, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28710860/coupling-the-pretreatment-and-hydrolysis-of-lignocellulosic-biomass-by-the-expression-of-beta-xylosidases
#9
Lucía Martín Pérez, Laura Benítez Casanova, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Sandra Gavaldá Martín, Laura Ledesma-García, Noelia Valbuena, Bruno Díez García, Francisco Manuel Reyes-Sosa
Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy intensity and chemical consumption in the pretreatment step are well known, the reduction of the severity of the process strongly affects the enzymatic hydrolysis yield. To explore the formulation requirements of the well known cellulolytic cocktail from Myceliophthora thermophila on mild pretreated raw materials, this cocktail was tested on steam exploded corn stover without acid impregnation...
July 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28707727/in-vitro-renin-angiotensin-system-inhibition-and-in-vivo-antihypertensive-activity-of-peptide-fractions-from-lima-bean-phaseolus-lunatus-l
#10
Norma A Ciau-Solís, Juan J Acevedo-Fernández, David Betancur-Ancona
BACKGROUND: The renin-angiotensin system is key in the physiopathology of arterial hypertension because it converts angiotensin I, via angiotensin I-converting enzyme (ACE), into angiotensin II. In vitro analyses were done of the ACE-inhibitory and renin-inhibitory activities of peptide fractions isolated by enzymatic hydrolysis of Lima bean (Phaseolus lunatus) protein. Antihypertensive activity was confirmed in vivo using a rat model. RESULTS: Lima bean protein was hydrolyzed with one of two sequential enzymatic systems (pepsin-pancreatin or Alcalase®-Flavourzyme®)...
July 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28704940/effects-of-protein-iron-complex-concentrate-supplementation-on-iron-metabolism-oxidative-and-immune-status-in-preweaning-calves
#11
Robert Kupczyński, Michał Bednarski, Kinga Śpitalniak, Krystyna Pogoda-Sewerniak
The objective of this study was to determine the effects of feeding protein-iron complex (PIC) on productive performance and indicators of iron metabolism, hematology parameters, antioxidant and immune status during first 35 days of a calf's life. Preparation of the complex involved enzymatic hydrolysis of milk casein (serine protease from Yarrowia lipolytica yeast). Iron chloride was then added to the hydrolyzate and lyophilizate. Calves were divided into treated groups: LFe (low iron dose) 10 g/day calf of protein-iron complex, HFe (height iron dose) 20 g/day calf, and control group...
July 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28704184/enzymatic-hydrolysis-of-water-extractable-polysaccharides-from-leaves-of-plantago-major-l
#12
Paolina K Lukova, Diana P Karcheva-Bahchevanska, Veselin P Bivolarski, Rumen D Mladenov, Ilia N Iliev, Mariana M Nikolova
BACKGROUND: Plantago major L. leaves have been used for centuries by the traditional medicine in the treatment of infectious disorders of the respiratory, urinary and digestive tracts. Researchers have reported that hot water extracts of Plantago major possess a broad-spectrum of anticancer, antioxidant and antiviral activities, as well as activities which modulate cell-mediated immunity. Their beneficial properties may be due to the significant content of polysaccharides. The polysaccharides that have been isolated from the leaves of Plantago major L...
June 1, 2017: Folia Medica
https://www.readbyqxmd.com/read/28702672/chitosan-stabilized-platinum-nanoparticles-as-effective-oxidase-mimics-for-colorimetric-detection-of-acid-phosphatase
#13
Hao-Hua Deng, Xiu-Ling Lin, Yin-Huan Liu, Ke-Lin Li, Qiong-Qiong Zhuang, Hua-Ping Peng, Ai-Lin Liu, Xing-Hua Xia, Wei Chen
Capping molecules on the surface of nanomaterials not only enhance the dispersion and stability of nanomaterials but also greatly facilitate their surface modification and biological applications. However, most capping molecules can severely block the active sites of the catalytic core, thereby decreasing the enzymatic activity of nanomaterial-based enzyme mimics. This work demonstrates the superiority of chitosan (Ch) as a capping molecule for synthesizing catalytic platinum nanoparticles (PtNPs). The experimental results show that Ch simultaneously exhibits an excellent stabilizing effect and enhances the oxidase-like activity of PtNPs...
July 12, 2017: Nanoscale
https://www.readbyqxmd.com/read/28702325/the-human-digestive-tract-has-proteases-capable-of-gluten-hydrolysis
#14
Sergio Gutiérrez, Jenifer Pérez-Andrés, Honorina Martínez-Blanco, Miguel Angel Ferrero, Luis Vaquero, Santiago Vivas, Javier Casqueiro, Leandro B Rodríguez-Aparicio
OBJECTIVE: To identify, purify, and characterize the proteins responsible for glutenase activity in the feces of healthy subjects and patients with celiac disease (CD). METHODS: Sixteen subjects were included in this study; 8 were healthy with no known food intolerances, and 8 were treated CD patients on a gluten-free diet. Fecal samples were homogenized, and precipitated proteins were purified by chromatography. Glutenase activity was evaluated by bioassays, zymography, and high-performance liquid chromatography with immunogenic 33-mer, 19-mer, and 13-mer gliadin peptides...
July 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28702082/enzymatic-degradation-of-sulfite-pulped-softwoods-and-the-role-of-lpmos
#15
Piotr Chylenski, Dejan M Petrović, Gerdt Müller, Marie Dahlström, Oskar Bengtsson, Martin Lersch, Matti Siika-Aho, Svein Jarle Horn, Vincent G H Eijsink
BACKGROUND: Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass, especially the discovery of lytic polysaccharide monooxygenases (LPMOs), have opened new perspectives for process design and optimization. Softwood biomass is an abundant resource in many parts of the world, including Scandinavia, but efficient pretreatment and subsequent enzymatic hydrolysis of softwoods are challenging. Sulfite pulping-based pretreatments, such as in the BALI™ process, yield substrates that are relatively easy to degrade...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28702081/limitation-of-cellulose-accessibility-and-unproductive-binding-of-cellulases-by-pretreated-sugarcane-bagasse-lignin
#16
Germano Siqueira, Valdeir Arantes, Jack N Saddler, André Ferraz, Adriane M F Milagres
BACKGROUND: The effectiveness of the enzymatic hydrolysis of cellulose in plant cell wall is strongly influenced by the access of enzymes to cellulose, which is at least in part limited by the presence of lignin. Although physicochemical treatments preceding the enzymatic catalysis significantly overcome this recalcitrance, the residual lignin can still play a role in the process. Lignin is suggested to act as a barrier, hindering cellulose and limiting the access of the enzymes. It can also unspecifically bind cellulases, reducing the amount of enzymes available to act on cellulose...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28698148/enzymatic-characterization-of-a-novel-xaa-pro-aminopeptidase-xpma-from-aspergillus-oryzae-expressed-in-escherichia-coli
#17
Mayumi Matsushita-Morita, Sawaki Tada, Satoshi Suzuki, Ryota Hattori, Ken-Ichi Kusumoto
Xaa-Pro aminopeptidases are peptidases responsible for the cleavage of any amino acid N-terminally adjacent to a proline residue. We identified a gene encoding a putative Xaa-Pro aminopeptidase in the genome of the filamentous fungus Aspergillus oryzae (genome database number: AO090701000720) and named this gene xpmA. We produced its enzyme in a C-terminally His6-tag-fused form in an Escherichia coli expression system and purified it. The purified recombinant XpmA (rXpmA) showed hydrolysis activity toward Xaa-Pro-oligopeptides, especially the two dipeptides Ala-Pro and Phe-Pro...
July 8, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28698130/enzymatic-hydrolysis-of-poly-ethyleneterephthalate-used-for-and-analysed-by-pore-modification-of-track-etched-membranes
#18
Caroline Gamerith, Martyna Gajda, Andreas Ortner, Enrique Herrero Acero, Georg M Guebitz, Mathias Ulbricht
The potential of limited enzymatic poly(ethylene terephthalate) (PET) surface hydrolysis for the modification of track-etched (TE) membranes was investigated. Cutinases 1 and 2 from Thermobifida cellulosilytica as well as a fusion protein of cutinase 1 with the polymer binding module from the polyhydroxyalkanoate depolymerase of Alcaligenes faecalis (Thc_Cut1_PBM) were shown to hydrolyse highly crystalline PET TE membranes with a pore diameter of ∼120nm at very narrow size distribution. Furthermore the effects of surface chemistry were investigated by comparison of enzymatic hydrolysis by Thc_Cut1_PBM of "as received" PET TE membranes with two surface functionalized versions towards a "hydrophilic" and a more "hydrophobic" surface...
July 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28696713/modulation-of-the-binding-affinity-of-polyzwitterion-conjugated-protein-by-ion-specific-effects-in-crowded-environments
#19
Wangqin Song, Jie Zhu, Lvdan Liu, Guangming Liu
Macromolecular crowding could influence the binding affinity of the polyzwitterion-conjugated proteins. Herein, the hydrolysis of N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide by the poly(carboxybetaine) conjugated α-chymotrypsin (PCT) was employed as a model system to investigate the modulation of the binding affinity of the polyzwitterion-conjugated proteins by the ion-specific effects in the crowded environments. In comparison with the bare α-chymotrypsin (BCT), the binding affinity of the PCT to the peptide is stronger in the dilute solutions but becomes weaker in the crowded environments...
July 11, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28691595/application-of-hydrolases-and-probiotic-pediococcus-acidilactici-baltbio01-strain-for-cereal-by-products-conversion-to-bioproduct-for-food-feed
#20
Elena Bartkiene, Vadims Bartkevics, Vita Krungleviciute, Grazina Juodeikiene, Daiva Zadeike, Violeta Baliukoniene, Bronius Bakutis, Rasa Zelvyte, Antonello Santini, Dalia Cizeikiene
The aim of this study was to apply the enzymatic treatment and fermentation by Pediococcus acidilactici BaltBio01 strain for industrial cereal by-products conversion to food/feed bioproducts with high amount of probiotic lactic acid bacteria (LAB). LAB propagated in potato media and spray-dried remained viable during 12 months (7.0 log10 cfu/g) of storage and was used as a starter for cereal by-products fermentation. The changes of microbial profile, biogenic amines (BAs), mycotoxins, lactic acid (L(+)/D(-)), lignans and alkylresorcinols (ARs) contents in fermented cereal by-product were analysed...
July 9, 2017: International Journal of Food Sciences and Nutrition
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