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Enzymatic hydrolysis

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https://www.readbyqxmd.com/read/28088097/ionic-liquid-pretreatment-of-cassava-residues-for-the-cogeneration-of-fermentative-hydrogen-and-methane
#1
Jun Cheng, Jiabei Zhang, Richen Lin, Jianzhong Liu, Li Zhang, Kefa Cen
An ionic liquid of N-methylmorpholine-N-oxide (NMMO) was used to effectively pretreat cassava residues for the efficient enzymatical hydrolysis and cogeneration of fermentative hydrogen and methane. The reducing sugar yield of enzymolysed cassava residues with NMMO pretreatment improved from 36 to 42g/100g cassava residues. Scanning electron microscopy images revealed the formation of deep grooves (∼4μm wide) and numerous pores in the cassava residues pretreated with NMMO. X-ray diffraction patterns showed that the crystallinity coefficient of NMMO-pretreated cassava residues decreased from 40 to 34...
January 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28081523/enhanced-enzymatic-hydrolysis-and-acetone-butanol-ethanol-fermentation-of-sugarcane-bagasse-by-combined-diluted-acid-with-oxidate-ammonolysis-pretreatment
#2
Hailong Li, Lian Xiong, Xuefang Chen, Can Wang, Gaoxiang Qi, Chao Huang, Mutan Luo, Xinde Chen
This study aims to propose a biorefinery pretreatment technology for the bioconversion of sugarcane bagasse (SB) into biofuels and N-fertilizers. Performance of diluted acid (DA), aqueous ammonia (AA), oxidate ammonolysis (OA) and the combined DA with AA or OA were compared in SB pretreatment by enzymatic hydrolysis, structural characterization and acetone-butanol-ethanol (ABE) fermentation. Results indicated that DA-OA pretreatment improves the digestibility of SB by sufficiently hydrolyzing hemicellulose into fermentable monosaccharides and oxidating lignin into soluble N-fertilizer with high nitrogen content (11...
January 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28078256/volatile-compound-physicochemical-and-antioxidant-properties-of-beany-flavor-removed-soy-protein-isolate-hydrolyzates-obtained-from-combined-high-temperature-pre-treatment-and-enzymatic-hydrolysis
#3
Sang-Hun Yoo, Yoon Hyuk Chang
The present study investigated the volatile compound, physicochemical, and antioxidant properties of beany flavor-removed soy protein isolate (SPI) hydrolyzates produced by combined high temperature pre-treatment and enzymatic hydrolysis. Without remarkable changes in amino acid composition, reductions of residual lipoxygenase activity and beany flavor-causing volatile compounds such as hexanol, hexanal, and pentanol in SPI were observed after combined heating and enzymatic treatments. The degree of hydrolysis, emulsion capacity and stability, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, and superoxide radical scavenging activity of SPI were significantly increased, but the magnitudes of apparent viscosity, consistency index, and dynamic moduli (G', G″) of SPI were significantly decreased after the combined heating and enzymatic treatments...
December 2016: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28069243/effect-of-combined-ultrasonic-and-alkali-pretreatment-on-enzymatic-preparation-of-angiotensin-converting-enzyme-ace-inhibitory-peptides-from-native-collagenous-materials
#4
Yuhao Zhang, Liang Ma, Luyun Cai, Yi Liu, Jianrong Li
The combined effect of ultrasonic and alkali pretreatment for the hydrolysis of native collagenous materials and release of ACE inhibitory peptides was investigated. The ultrasonic and alkali pretreatment of pig skin could accelerate the release of the ACE inhibitory peptides from the triple helix of collagen in early stages of hydrolysis. Furthermore, the pretreatment could also accelerate collapse of the triple helix and release more ACE inhibitory peptides during hydrolysis than collagen samples left untreated...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28069197/low-strength-ultrasonication-positively-affects-methanogenic-granules-toward-higher-ad-performance-hydrolytic-enzyme-excretions
#5
Si-Kyung Cho, Yeo-Myeong Yun, Seung Gu Shin
In our previous studies, enhanced methane (CH4) production using low-strength ultrasonication was achieved and the results were evidenced by physico-chemical and molecular biological approaches. As a final continuation study, the effects of low-strength ultrasonication on the activities of hydrolytic enzymes (amylase, cellulase, and protease) were investigated on methanogenic granules given that hydrolysis regulates the whole anaerobic digestion (AD) process. Up to 213% enhanced hydrolytic enzyme activities were observed, and they seem to be highly related to the enhanced CH4 production...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28069129/antioxidant-activity-measurement-and-potential-antioxidant-peptides-exploration-from-hydrolysates-of-novel-continuous-microwave-assisted-enzymolysis-of-the-scomberomorus-niphonius-protein
#6
Yipeng Huang, Guihua Ruan, Zhijun Qin, Haiyun Li, Yanjie Zheng
A novel continuous microwave-assisted enzymatic digestion (cMAED) method is proposed for the digestion of protein from Scomberomorus niphonius to obtain potential antioxidant peptides. In this study, bromelain was found to have a high capacity for the digestion of the Scomberomorus niphonius protein. The following cMAED conditions were investigated: protease species, microwave power, temperature, bromelain content, acidity of the substrate solution, and incubation time. At 400W, 40°C, 1500U·g(-1) bromelain, 20% substrate concentration, pH 6...
May 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28068591/feasibility-of-reusing-the-black-liquor-for-enzymatic-hydrolysis-and-ethanol-fermentation
#7
Wen Wang, Xiaoyan Chen, Xuesong Tan, Qiong Wang, Yunyun Liu, Minchao He, Qiang Yu, Wei Qi, Yu Luo, Xinshu Zhuang, Zhenhong Yuan
The black liquor (BL) generated in the alkaline pretreatment process is usually thought as the environmental pollutant. This study found that the pure alkaline lignin hardly inhibited the enzymatic hydrolysis of cellulose (EHC), which led to the investigation on the feasibility of reusing BL as the buffer via pH adjustment for the subsequent enzymatic hydrolysis and fermentation. The pH value of BL was adjusted from 13.23 to 4.80 with acetic acid, and the alkaline lignin was partially precipitated. It deposited on the surface of cellulose and negatively influenced the EHC via blocking the access of cellulase to cellulose and adsorbing cellulase...
December 23, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28065569/pretreatment-of-rice-straw-by-ultrasound-assisted-fenton-process
#8
Zi-Yao Xiong, Yuan-Hang Qin, Jia-Yu Ma, Li Yang, Zai-Kun Wu, Tie-Lin Wang, Wei-Guo Wang, Cun-Wen Wang
Fenton's reagent, ultrasound, and the combination of Fenton's reagent and ultrasound were used to pretreat rice straw (RS) to increase its enzymatic digestibility for saccharification. The characterization shows that compared with ultrasound, Fenton's reagent pretreatment was more efficient in increasing the specific surface area and decreasing the degree of polymerization (DP) of RS. The enzymatic hydrolysis results showed that the RS pretreated by ultrasound-assisted Fenton's reagent (U/F-RS), which exhibited the largest specific surface area and the lowest DP value, had the highest enzymatic activity, and the amount of reducing sugar released from U/F-RS at 48h of enzymatic saccharification is about 4 times as large as that from raw RS and 1...
December 30, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28065261/expression-purification-and-enzymatic-characterization-of-intramembrane-proteases
#9
R Zhou, Y Shi, G Yang
Intramembrane proteases catalyze peptide bond hydrolysis in the lipid bilayer and play a key role in numerous cellular processes. These integral membrane enzymes consist of four classes: site-2 protease (S2P), rhomboid serine protease, Rce1-type glutamyl protease, and aspartyl protease exemplified by presenilin and signal peptide peptidase (SPP). Structural elucidation of these enzymes is important for mechanistic understanding of their functions, particularly their roles in cell signaling and debilitating diseases such as Parkinson's disease and Alzheimer's disease...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28063358/chemical-input-reduction-in-the-arabinoxylan-and-lignocellulose-alkaline-extraction-and-xylooligosaccharides-production
#10
Franciane Cristina de Figueiredo, Ana Flavia Azevedo Carvalho, Michel Brienzo, Tania Sila Campioni, Pedro de Oliva-Neto
Lignocellulosic material breakdown by hydrolysis is an important step to open new perspectives for bioenergy and special foods production like prebiotic xylooligosaccharides. Improvement of lignocellulose and arabinoxylan alkaline extraction from sugarcane bagasse and enzymatic hydrolysis were performed. Treatments 1 (10% KOH at 70°C), 3 (5% KOH at 121°C) and ZD method (24% KOH at 35°C) showed solid lignocellulose recovery of respectively 75.2%, 74.2% and 73%. A range of 24.8-27% extracted material with high arabinoxylan content (72...
December 26, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28063146/pretreatment-optimization-of-the-biomass-of-microcystis-aeruginosa-for-efficient-bioethanol-production
#11
Muhammad Imran Khan, Moon Geon Lee, Jin Hyuk Shin, Jong Deog Kim
Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28062914/determination-of-kinetics-and-heat-of-hydrolysis-for-non-homogenous-substrate-by-isothermal-calorimetry
#12
D Tafoukt, A Soric, J-C Sigoillot, J-H Ferrasse
The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temperature of the enzymes used for lignocellulosic molecules hydrolysis was determined. Thus, the amount of substrate-to-enzyme ratio was highlighted as an important parameter of the hydrolysis yield...
January 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28062240/isolation-and-further-structural-characterization-of-lignins-from-the-valonea-of-quercus-variabilis
#13
Lina Yang, Dongmei Wang, Dan Zhou, Yawei Zhang, Tingting Yang
The isolation process of alkali lignin (AL) from the valonea of Quercus variabilis Blume was optimized (liquid/solid ratio, 12.21; isolation time, 4.21h; isolation temperature, 42.21°C; and alkali concentration, 0.85mol/L) using the response surface method (RSM), with the highest isolation rate obtained being 22.67%. Then, the apparent structures of AL, enzymatic hydrolysis lignin (CEL) and milled wood lignin (MWL) were studied by scanning electron microscopy (SEM), which indicated that the isolation processes of AL and CEL caused some damage to the apparent structure of lignin...
January 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28061394/surface-lignin-change-pertaining-to-the-integrated-process-of-dilute-acid-pre-extraction-and-mechanical-refining-of-poplar-wood-chips-and-its-impact-on-enzymatic-hydrolysis
#14
Wei Liu, Wei Chen, Qingxi Hou, Jinping Zhang, Bing Wang
Dilute acid pre-extraction enhanced the mechanically refined poplar pulp substrates' enzymatic hydrolysis efficiency obviously. The results showed that the surface lignin distribution was changed significantly in residual wood chips and pulp substrates, and the surface lignin distribution showed important impact on the following enzymatic hydrolysis. Acid pre-extraction can lead to a redistribution of lignin in fiber cell walls, i.e., the lignin was degraded and migrated to fiber surface in the form of re-deposited lignin and pseudo-lignin...
December 28, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28058278/a-%C3%AE-mannanase-with-a-lysozyme-like-fold-and-a-novel-molecular-catalytic-mechanism
#15
Yi Jin, Marija Petricevic, Alan John, Lluís Raich, Huw Jenkins, Leticia Portela De Souza, Fiona Cuskin, Harry J Gilbert, Carme Rovira, Ethan D Goddard-Borger, Spencer J Williams, Gideon J Davies
The enzymatic cleavage of β-1,4-mannans is achieved by endo-β-1,4-mannanases, enzymes involved in germination of seeds and microbial hemicellulose degradation, and which have increasing industrial and consumer product applications. β-Mannanases occur in a range of families of the CAZy sequence-based glycoside hydrolase (GH) classification scheme including families 5, 26, and 113. In this work we reveal that β-mannanases of the newly described GH family 134 differ from other mannanase families in both their mechanism and tertiary structure...
December 28, 2016: ACS Central Science
https://www.readbyqxmd.com/read/28053668/study-of-traits-and-recalcitrance-reduction-of-field-grown-comt-down-regulated-switchgrass
#16
Mi Li, Yunqiao Pu, Chang Geun Yoo, Erica Gjersing, Stephen R Decker, Crissa Doeppke, Todd Shollenberger, Timothy J Tschaplinski, Nancy L Engle, Robert W Sykes, Mark F Davis, Holly L Baxter, Mitra Mazarei, Chunxiang Fu, Richard A Dixon, Zeng-Yu Wang, C Neal Stewart, Arthur J Ragauskas
BACKGROUND: The native recalcitrance of plants hinders the biomass conversion process using current biorefinery techniques. Down-regulation of the caffeic acid O-methyltransferase (COMT) gene in the lignin biosynthesis pathway of switchgrass reduced the thermochemical and biochemical conversion recalcitrance of biomass. Due to potential environmental influences on lignin biosynthesis and deposition, studying the consequences of physicochemical changes in field-grown plants without pretreatment is essential to evaluate the performance of lignin-altered plants...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28053529/co-self-assembly-of-cationic-microparticles-to-deliver-pegfp-znf580-for-promoting-the-transfection-and-migration-of-endothelial-cells
#17
Yakai Feng, Mengyang Guo, Wen Liu, Xuefang Hao, Wei Lu, Xiangkui Ren, Changcan Shi, Wencheng Zhang
The gene transfection efficiency of polyethylenimine (PEI) varies with its molecular weight. Usually, high molecular weight of PEI means high gene transfection, as well as high cytotoxicity in gene delivery in vivo. In order to enhance the transfection efficiency and reduce the cytotoxicity of PEI-based gene carriers, a novel cationic gene carrier was developed by co-self-assembly of cationic copolymers. First, a star-shaped copolymer poly(3(S)-methyl-morpholine-2,5-dione-co-lactide) (P(MMD-co-LA)) was synthesized using D-sorbitol as an initiator, and the cationic copolymer (P(MMD-co-LA)-g-PEI) was obtained after grafting low-molecular weight PEI...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28045609/preparation-of-cellulase-concoction-employing-differential-adsorption-phenomenon
#18
Sachinkumar Birhade, Mukesh Pednekar, Shilpa Sagwal, Annamma Odaneth, Arvind Lali
Controlled depolymerization of cellulose is essential for the production of valuable cellooligosaccharides and cellobiose from lignocellulosic biomass. However, enzymatic cellulose hydrolysis involves multiple synergistically acting enzymes, making difficult to control the depolymerization process and generate desired product. This work exploits the varying adsorption properties of the cellulase components to the cellulosic substrate and aims to control the enzyme activity. Cellulase adsorption was favored on pretreated cellulosic biomass as compared to synthetic cellulose...
January 3, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28040870/innovative-methods-to-generate-clean-sugar-stream-from-biomass-feedstocks-for-efficient-fermentation
#19
REVIEW
Jung-Eun Lee, Yadhu N Guragain, Krishna P Bastola, Praveen V Vadlani
Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural (HMF) and furfural, were effectively removed using an appropriate detoxification method using 1% (w/v) activated charcoal. The resin mixture containing cationic exchangers (DOWEX 50WX8) and anionic exchangers (IRA 743) in 7:3 ratio at pH 2...
December 31, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28040661/foam-fractionation-as-a-tool-to-study-the-air-water-interface-structure-function-relationship-of-wheat-gluten-hydrolysates
#20
Arno G B Wouters, Ine Rombouts, Nele Schoebrechts, Ellen Fierens, Kristof Brijs, Christophe Blecker, Jan A Delcour
Enzymatic hydrolysis of wheat gluten protein improves its solubility and produces hydrolysates with foaming properties which may find applications in food products. First, we here investigated whether foam-liquid fractionation can concentrate wheat gluten peptides with foaming properties. Foam and liquid fractions had high and very low foam stability (FS), respectively. In addition, foam fractions were able to decrease surface tension more pronouncedly than un-fractionated samples and liquid fractions, suggesting they are able to arrange themselves more efficiently at an interface...
December 22, 2016: Colloids and Surfaces. B, Biointerfaces
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