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https://www.readbyqxmd.com/read/28346369/metabolic-impact-of-light-phase-restricted-fructose-consumption-is-linked-to-changes-in-hypothalamic-ampk-phosphorylation-and-melatonin-production-in-rats
#1
Juliana de Almeida Faria, Thiago Matos F de Araújo, Daniela S Razolli, Letícia Martins Ignácio-Souza, Dailson Nogueira Souza, Silvana Bordin, Gabriel Forato Anhê
Recent studies show that the metabolic effects of fructose may vary depending on the phase of its consumption along with the light/dark cycle. Here, we investigated the metabolic outcomes of fructose consumption by rats during either the light (LPF) or the dark (DPF) phases of the light/dark cycle. This experimental approach was combined with other interventions, including restriction of chow availability to the dark phase, melatonin administration or intracerebroventricular inhibition of adenosine monophosphate-activated protein kinase (AMPK) with Compound C...
March 27, 2017: Nutrients
https://www.readbyqxmd.com/read/28345624/the-effects-of-self-selected-light-dark-cycles-and-social-constraints-on-human-sleep-and-circadian-timing-a-modeling-approach
#2
Anne C Skeldon, Andrew J K Phillips, Derk-Jan Dijk
Why do we go to sleep late and struggle to wake up on time? Historically, light-dark cycles were dictated by the solar day, but now humans can extend light exposure by switching on artificial lights. We use a mathematical model incorporating effects of light, circadian rhythmicity and sleep homeostasis to provide a quantitative theoretical framework to understand effects of modern patterns of light consumption on the human circadian system. The model shows that without artificial light humans wakeup at dawn...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345351/shape-memory-characteristics-of-o157-antigenic-cavities-generated-on-nanocomposites-consisting-of-copolymer-encapsulated-gold-nanoparticles
#3
Takamasa Kinoshita, Dung Quang Nguyen, Dung Quynh Le, Kengo Ishiki, Hiroshi Shiigi, Tsutomu Nagaoka
Nanometer-sized composite particles, which consisted of gold nanoparticles encapsulated by an N-isopropylacrylamide copolymer, were successfully synthesized using a one-step process. Shape complementary cavities of the O157-antigen were formed on the composite utilizing temperature-dependent affinity changes of the copolymer. The composite bound to enterohemorrhagic Escherichia coli (E. coli) O157 at 298 K and enhanced light-scattering intensity of the cell due to the optical properties of the gold nanoparticles...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28344388/isolation-and-purification-of-all-trans-diadinoxanthin-and-all-trans-diatoxanthin-from-diatom-phaeodactylum-tricornutum
#4
Paulina Kuczynska, Malgorzata Jemiola-Rzeminska
Two diatom-specific carotenoids are engaged in the diadinoxanthin cycle, an important mechanism which protects these organisms against photoinhibition caused by absorption of excessive light energy. A high-performance and economical procedure of isolation and purification of diadinoxanthin and diatoxanthin from the marine diatom Phaeodactylum tricornutum using a four-step procedure has been developed. It is based on the use of commonly available materials and does not require advanced technology. Extraction of pigments, saponification, separation by partition and then open column chromatography, which comprise the complete experimental procedure, can be performed within 2 days...
2017: Journal of Applied Phycology
https://www.readbyqxmd.com/read/28344099/mechanisms-of-resistance-to-selective-estrogen-receptor-down-regulator-in-metastatic-breast-cancer
#5
REVIEW
Doudou Huang, Fang Yang, Yucai Wang, Xiaoxiang Guan
Based on the prominent role estrogen receptor (ER) plays in breast cancer, endocrine therapy has been developed to block the ER pathway and has shown great effectiveness. Fulvestrant, the first selective ER down-regulator (SERD), was demonstrated to completely suppress ERα and notably efficient. However, resistance to fulvestrant occurs, either intrinsic or acquired during the treatment. Several potential mechanisms inducing fulvestrant resistance have been proposed, composed of activated ERα-independent compensatory growth factor signaling, stimulated downstream kinases, altered cell cycle mediators etcetera...
March 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28343265/effect-of-10-cerium-chloride-on-artificial-caries-lesions-of-human-enamel-evaluated-using-quantitative-light-induced-fluorescence-an-in-vitro-study
#6
R Jaisingh, R Shanbhog, B Nandlal, M Thippeswamy
AIM: To evaluate the effect of 10% cerium chloride intervention on pre-demineralised human enamel subjected to cycles of de- and remineralisation. METHODS: This was an in vitro, comparative type study of 60 human enamel samples that were randomly divided into control and test groups. The samples were then subjected to a cycle of demineralisation followed by intervention with respective treatment solutions (control group: placebo solution; test: 10% cerium chloride) for 30 s under constant agitation and then to a 2nd cycle of demineralisation followed by remineralisation using artificial saliva...
March 25, 2017: European Archives of Paediatric Dentistry: Official Journal of the European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/28343123/estimation-of-red-light-running-frequency-using-high-resolution-traffic-and-signal-data
#7
Peng Chen, Guizhen Yu, Xinkai Wu, Yilong Ren, Yueguang Li
Red-light-running (RLR) emerges as a major cause that may lead to intersection-related crashes and endanger intersection safety. To reduce RLR violations, it's critical to identify the influential factors associated with RLR and estimate RLR frequency. Without resorting to video camera recordings, this study investigates this important issue by utilizing high-resolution traffic and signal event data collected from loop detectors at five intersections on Trunk Highway 55, Minneapolis, MN. First, a simple method is proposed to identify RLR by fully utilizing the information obtained from stop bar detectors, downstream entrance detectors and advance detectors...
March 23, 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/28341289/long-term-culture-with-lipopolysaccharide-induces-dose-dependent-cytostatic-and-cytotoxic-effects-in-thp-1-monocytes
#8
Jennifer Mytych, Maria Romerowicz-Misielak, Marek Koziorowski
Monocytes act as a first line of defence against invading pathogens and their dysfunctions seem to be a key factor in many immune disorders. However, the data on mechanisms underlying these dysfunctions remain elusive. In this study, we evaluated the effects of long-term (168h) lipopolysaccharide exposure on monocytes at low density cultures (1×10(5)cells/ml). Treatment with low dose LPS (≤5μg/ml) resulted in oxidative stress induction followed by p21 pathway activation, permanent cell cycle arrest and SASP development...
March 21, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28341096/anoxic-oscillating-mbr-for-photosynthetic-bacteria-harvesting-and-high-salinity-wastewater-treatment
#9
Lei Qin, Qiuhua Liu, Qin Meng, Zheng Fan, Jinzhe He, Tao Liu, Chong Shen, Guoliang Zhang
In this study, photosynthetic bacteria (PSB) were first harvested by MBR with pendulum type oscillation (PTO) hollow fiber module in succession and on a large scale. Based on unique properties of PSB, PSB/MBR was successfully applied for high-salinity wastewater treatment. Compared with control PSB-MBR (CMBR), PSB/PTO-MBR exhibited more excellent organics removal, which was mainly attributed to much higher biomass production for utilization. Meanwhile, the influence of light irradiation and aeration on activity of PSB was investigated in detail...
January 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28340452/a-study-of-photosynthetic-biogas-upgrading-based-on-a-high-rate-algal-pond-under-alkaline-conditions-influence-of-the-illumination-regime
#10
Mariana Franco-Morgado, Cynthia Alcántara, Adalberto Noyola, Raúl Muñoz, Armando González-Sánchez
Microalgal-bacterial processes have emerged as environmental friendly systems for the cost-effective treatment of anaerobic effluents such as biogas and nutrients-laden digestates. Environmental parameters such as temperature, irradiation, nutrient concentration and pH effect the performance of the systems. In this paper, the potential of a microalgal-bacterial photobioreactor operated under high pH (≈9.5) and high alkalinity to convert biogas into biomethane was evaluated. The influence of the illumination regime (continuous light supply vs 12h/12h light/dark cycles) on the synthetic biogas upgrading efficiency, biomass productivity and nutrient removal efficiency was assessed in a High-Rate Algal Pond interconnected to a biogas absorption bubble column...
March 20, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28338803/vertical-and-seasonal-variations-in-temperature-responses-of-leaf-respiration-in-a-chamaecyparis-obtusa-canopy
#11
Masatake G Araki, Koichiro Gyokusen, Takuya Kajimoto
Leaf respiration (R) is a major component of carbon balance in forest ecosystems. Clarifying the variability of leaf R within a canopy is essential for predicting the impact of global warming on forest productivity and the potential future function of the forest ecosystem as a carbon sink. We examined vertical and seasonal variations in short-term temperature responses of leaf R as well as environmental factors (light and mean air temperature) and physiological factors [leaf nitrogen (N), leaf mass per area (LMA), and shoot growth] in the canopy of a 10-year-old stand of hinoki cypress [Chamaecyparis obtusa (Sieb...
February 23, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28338143/mastering-the-non-equilibrium-assembly-and-operation-of-molecular-machines
#12
Cristian Pezzato, Chuyang Cheng, J Fraser Stoddart, R Dean Astumian
In mechanically interlocked compounds, such as rotaxanes and catenanes, the molecules are held together by mechanical rather than chemical bonds. These compounds can be engineered to have several well-defined mechanical states by incorporating recognition sites between the different components. The rates of the transitions between the recognition sites can be controlled by introducing steric "speed bumps" or electrostatically switchable gates. A mechanism for the absorption of energy can also be included by adding photoactive, catalytically active, or redox-active recognition sites, or even charges and dipoles...
March 24, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28337554/successive-relaxation-cycles-during-long-time-cell-aggregate-rounding-after-uni-axial-compression
#13
Ivana Pajic-Lijakovic, Milan Milivojevic
The mean features of cell surface rearrangement during cell aggregate rounding after uni-axial compression between parallel plates are considered. This is based on long-time rheological modeling approaches in order to shed further light on collective cell migration. Many aspects of cell migration at the supra-cellular level, such as the coordination between surrounding migrating cell groups that leads to uncorrelated motility, have remained unclear. Aggregate shape changes during rounding are considered depending on the size and homogeneity of 2-D and 3-D cell aggregates...
March 23, 2017: Journal of Biological Physics
https://www.readbyqxmd.com/read/28337118/toxicological-risk-at-workplace-and-toxicity-as-life-cycle-assessment-impact-category-substitution-of-solvents-as-an-example
#14
Thomas Schupp, Philipp Alexander Georg, Guenter Kirstein
Substitution of hazardous substances against less hazardous ones is a central requirement of the European Chemical Regulation REACH (European Regulation 1907/2006/EC). Hazardous substances emitted from products may not only affect the worker; drift off and distribution in the environment may finally result in exposure of the general population. This potential threat to health is covered by the impact category "toxicity" in Life Cycle Assessments. In this paper, we present a case of a substitution of volatile organic compounds in a reactive varnish, and compare the "old" formulation with the "new" formulation against health risk to the worker, and concerning the Life Cycle Assessment impact category "toxicity"...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28334407/organismal-and-spatial-partitioning-of-energy-and-macronutrient-transformations-within-a-hypersaline-mat
#15
Jennifer M Mobberley, Stephen R Lindemann, Hans C Bernstein, James J Moran, Ryan S Renslow, Jerome Babauta, Dehong Hu, Haluk Beyenal, William C Nelson
Phototrophic mat communities are model ecosystems for studying energy cycling and elemental transformations because complete biogeochemical cycles occur over millimeter-to-centimeter scales. Characterization of energy and nutrient capture within hypersaline phototrophic mats has focused on specific processes and organisms, however little is known about community-wide distribution of and linkages between these processes. To investigate energy and macronutrient capture and flow through a structured community, the spatial and organismal distribution of metabolic functions within a compact hypersaline mat community from Hot Lake have been broadly elucidated through species-resolved metagenomics and geochemical, microbial diversity, and metabolic gradient measurements...
March 15, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28334286/differences-in-the-photosynthetic-plasticity-of-ferns-and-ginkgo-grown-in-experimentally-controlled-low-o2-co2-atmospheres-may-explain-their-contrasting-ecological-fate-across-the-triassic-jurassic-mass-extinction-boundary
#16
C Yiotis, C Evans-Fitz Gerald, J C McElwain
Background and Aims: Fluctuations in [CO 2 ] have been widely studied as a potential driver of plant evolution; however, the role of a fluctuating [O 2 ]:[CO 2 ] ratio is often overlooked. The present study aimed to investigate the inherent physiological plasticity of early diverging, extant species following acclimation to an atmosphere similar to that across the Triassic-Jurassic mass extinction interval (TJB, approx. 200 Mya), a time of major ecological change. Methods: Mature plants from two angiosperm ( Drimys winteri and Chloranthus oldhamii ), two monilophyte ( Osmunda claytoniana and Cyathea australis ) and one gymnosperm ( Ginkgo biloba ) species were grown for 2 months in replicated walk-in Conviron BDW40 chambers running at TJB treatment conditions of 16 % [O 2 ]-1900 ppm [CO 2 ] and ambient conditions of 21 % [O 2 ]-400 ppm [CO 2 ], and their physiological plasticity was assessed using gas exchange and chlorophyll fluorescence methods...
March 11, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28333924/csl-encodes-a-leucine-rich-repeat-protein-implicated-in-red-violet-light-signaling-to-the-circadian-clock-in-chlamydomonas
#17
Ayumi Kinoshita, Yoshimi Niwa, Kiyoshi Onai, Takashi Yamano, Hideya Fukuzawa, Masahiro Ishiura, Takuya Matsuo
The green alga Chlamydomonas reinhardtii shows various light responses in behavior and physiology. One such photoresponse is the circadian clock, which can be reset by external light signals to entrain its oscillation to daily environmental cycles. In a previous report, we suggested that a light-induced degradation of the clock protein ROC15 is a trigger to reset the circadian clock in Chlamydomonas. However, light signaling pathways of this process remained unclear. Here, we screened for mutants that show abnormal ROC15 diurnal rhythms, including the light-induced protein degradation at dawn, using a luciferase fusion reporter...
March 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28332561/inertial-bioluminescence-rhythms-at-the-capo-passero-km3net-italia-site-central-mediterranean-sea
#18
J Aguzzi, E Fanelli, T Ciuffardi, A Schirone, J Craig
In the deep sea, the sense of time is dependent on geophysical fluctuations, such as internal tides and atmospheric-related inertial currents, rather than day-night rhythms. Deep-sea neutrino telescopes instrumented with light detecting Photo-Multiplier Tubes (PMT) can be used to describe the synchronization of bioluminescent activity of abyssopelagic organisms with hydrodynamic cycles. PMT readings at 8 different depths (from 3069 to 3349 m) of the NEMO Phase 2 prototype, deployed offshore Capo Passero (Sicily) at the KM3NeT-Italia site, were used to characterize rhythmic bioluminescence patterns in June 2013, in response to water mass movements...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332019/comparative-tissue-transcriptomics-highlights-dynamic-differences-among-tissues-but-conserved-metabolic-transcript-prioritization-in-preparation-for-arousal-from-torpor
#19
Lori K Bogren, Katharine R Grabek, Gregory S Barsh, Sandra L Martin
During the hibernation season, 13-lined ground squirrels spend days to weeks in torpor with body temperatures near freezing then spontaneously rewarm. The molecular drivers of the drastic physiological changes that orchestrate and permit torpor are not well understood. Although transcription effectively ceases at the low body temperatures of torpor, previous work has demonstrated that some transcripts are protected from bulk degradation in brown adipose tissue (BAT), consistent with the importance of their protein products for metabolic heat generation during arousal from torpor...
March 23, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28331201/synchrony-of-plant-cellular-circadian-clocks-with-heterogeneous-properties-under-light-dark-cycles
#20
Masaaki Okada, Tomoaki Muranaka, Shogo Ito, Tokitaka Oyama
Individual cells in a plant can work independently as circadian clocks, and their properties are the basis of various circadian phenomena. The behaviour of individual cellular clocks in Lemna gibba was orderly under 24-h light/dark cycles despite their heterogeneous free-running periods (FRPs). Here, we reveal the entrainment habits of heterogeneous cellular clocks using non-24-h light/dark cycles (T-cycles). The cellular rhythms of AtCCA1::LUC under T = 16 h cycles showed heterogeneous entrainment that was associated with their heterogeneous FRPs...
March 22, 2017: Scientific Reports
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