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"malaria human challenge"

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https://www.readbyqxmd.com/read/22403305/real-time-quantitative-reverse-transcription-pcr-for-monitoring-of-blood-stage-plasmodium-falciparum-infections-in-malaria-human-challenge-trials
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Sean C Murphy, Jennifer L Prentice, Kathryn Williamson, Carolyn K Wallis, Ferric C Fang, Michal Fried, Cris Pinzon, Ruobing Wang, Angela K Talley, Stefan H I Kappe, Patrick E Duffy, Brad T Cookson
To detect pre-patent parasitemia, we developed a real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for the asexual 18S ribosomal RNA (rRNAs) of Plasmodium falciparum. Total nucleic acids extracted from whole blood were combined with control RNA and tested by qRT-PCR. The assay quantified > 98.7% of parasite-containing samples to ±0.5 log(10) parasites/mL of the nominal value without false positives. The analytical sensitivity was ≥ 20 parasites/mL. The coefficient of variation was 0...
March 2012: American Journal of Tropical Medicine and Hygiene
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