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JOURNAL ARTICLE
Alexander Lee, Marie A Bogoyevitch, David A Jans
Bimolecular fluorescence complementation utilizes the ability of two complementary nonfluorescent fragments to reconstitute and emit fluorescence when brought together through specific interaction of attached protein fragments of interest. It has been used in several different contexts to study protein-protein interaction. Here we apply the method for the first time to study interaction of the nuclear transporter importin α and its cargoes in a cellular context. By using image analysis to quantify the extent of nuclear complexation, it is possible to gain insight into the strength of interaction in cells...
2022: Methods in Molecular Biology
#22
JOURNAL ARTICLE
Fawzia Bardag-Gorce, Alissa Diaz, Robert Niihara, Jeremy Stark, Daileen Cortez, Alexander Lee, Richard Hoft, Yutaka Niihara
PURPOSE: The purpose of the present study is to investigate the expression of aldehyde dehydrogenases (ALDHs) in rabbit corneas with limbal stem cell deficiency (LSCD) and corneas treated with cultured autologous oral mucosa epithelial cell sheet CAOMECS designed to reconstruct the ocular surface with LSCD. METHODS: New Zealand white rabbit autologous oral mucosal epithelial cells were isolated from a buccal biopsy and cultured to be grafted back onto corneas of rabbit model of LSCD...
April 5, 2022: International Journal of Molecular Sciences
#23
RANDOMIZED CONTROLLED TRIAL
Mrinal M Gounder, Albiruni Abdul Razak, Neeta Somaiah, Sant Chawla, Javier Martin-Broto, Giovanni Grignani, Scott M Schuetze, Bruno Vincenzi, Andrew J Wagner, Bartosz Chmielowski, Robin L Jones, Richard F Riedel, Silvia Stacchiotti, Elizabeth T Loggers, Kristen N Ganjoo, Axel Le Cesne, Antoine Italiano, Xavier Garcia Del Muro, Melissa Burgess, Sophie Piperno-Neumann, Christopher Ryan, Mary F Mulcahy, Charles Forscher, Nicolas Penel, Scott Okuno, Anthony Elias, Lee Hartner, Tony Philip, Thierry Alcindor, Bernd Kasper, Peter Reichardt, Lore Lapeire, Jean-Yves Blay, Christine Chevreau, Claudia Maria Valverde Morales, Gary K Schwartz, James L Chen, Hari Deshpande, Elizabeth J Davis, Garth Nicholas, Stefan Gröschel, Helen Hatcher, Florence Duffaud, Antonio Casado Herráez, Roberto Diaz Beveridge, Giuseppe Badalamenti, Mikael Eriksson, Christian Meyer, Margaret von Mehren, Brian A Van Tine, Katharina Götze, Filomena Mazzeo, Alexander Yakobson, Aviad Zick, Alexander Lee, Anna Estival Gonzalez, Andrea Napolitano, Mark A Dickson, Dayana Michel, Changting Meng, Lingling Li, Jianjun Liu, Osnat Ben-Shahar, Dane R Van Domelen, Christopher J Walker, Hua Chang, Yosef Landesman, Jatin J Shah, Sharon Shacham, Michael G Kauffman, Steven Attia
PURPOSE: Antitumor activity in preclinical models and a phase I study of patients with dedifferentiated liposarcoma (DD-LPS) was observed with selinexor. We evaluated the clinical benefit of selinexor in patients with previously treated DD-LPS whose sarcoma progressed on approved agents. METHODS: SEAL was a phase II-III, multicenter, randomized, double-blind, placebo-controlled study. Patients age 12 years or older with advanced DD-LPS who had received two-five lines of therapy were randomly assigned (2:1) to selinexor (60 mg) or placebo twice weekly in 6-week cycles (crossover permitted)...
August 1, 2022: Journal of Clinical Oncology
#24
JOURNAL ARTICLE
Alexander Lee, Ali Farooqi, Eric Abreu, Divya Talwar, Kathleen Maguire
BACKGROUND: Millions of youth participate in baseball and softball in the United States and these youth account for a large number of pediatric sports injuries. This study sought to characterize annual, seasonal, and age-related trends in pediatric softball and baseball injuries presenting to the Emergency Department (ED). METHODS: The National Electronic Injury Surveillance System (NEISS) database was retrospectively examined for all softball and baseball injuries from 2010 to 2019 involving pediatric patients aged 7-21 years...
June 2023: Physician and Sportsmedicine
#25
JOURNAL ARTICLE
Micah Steffek, Elizabeth Helgason, Nataliya Popovych, Lionel Rougé, John M Bruning, Ke Sherry Li, Daniel J Burdick, Jianping Cai, Terry Crawford, Jing Xue, Willy Decurtins, Chunlin Fang, Felix Grubers, Michael J Holliday, Allyson Langley, Ann Petersen, Alexander Lee Satz, Aimin Song, Daniel Stoffler, Quentin Strebel, Jeffrey Y K Tom, Nicholas Skelton, Steven T Staben, Moreno Wichert, Melinda M Mulvihill, Erin C Dueber
Autophagy-related proteins (Atgs) drive the lysosome-mediated degradation pathway, autophagy, to enable the clearance of dysfunctional cellular components and maintain homeostasis. In humans, this process is driven by the mammalian Atg8 (mAtg8) family of proteins comprising the LC3 and GABARAP subfamilies. The mAtg8 proteins play essential roles in the formation and maturation of autophagosomes and the capture of specific cargo through binding to the conserved LC3-interacting region (LIR) sequence within target proteins...
January 5, 2022: Biochemistry
#26
JOURNAL ARTICLE
Kasey R Boehmer, Kathleen H Pine, Samantha Whitman, Paige Organick, Anjali Thota, Nataly R Espinoza Suarez, Christina M LaVecchia, Alexander Lee, Emma Behnken, Bjorg Thorsteinsdottir, Aditya S Pawar, Annika Beck, Elizabeth C Lorenz, Robert C Albright
BACKGROUND: Approximately 750,000 people in the U.S. live with end-stage kidney disease (ESKD); the majority receive dialysis. Despite the importance of adherence to dialysis, it remains suboptimal, and one contributor may be patients' insufficient capacity to cope with their treatment and illness burden. However, it is unclear what, if any, differences exist between patients reporting high versus low treatment and illness burden. METHODS: We sought to understand these differences using a mixed methods, explanatory sequential design...
2021: PloS One
#27
JOURNAL ARTICLE
Ali S Farooqi, Alexander Lee, Eric Abreu, Divya Talwar, Kathleen J Maguire
BACKGROUND: Baseball and softball are popular sports in the United States and are responsible for a large number of youth sports injuries each year. PURPOSE: To investigate recent differences in youth baseball and softball injuries evaluated in nationwide emergency departments. STUDY DESIGN: Descriptive epidemiology study. METHODS: The National Electronic Injury Surveillance System (NEISS) database was examined for softball and baseball injuries in pediatric patients (age, 7-21 years) from 2010 through 2019...
December 2021: Orthopaedic Journal of Sports Medicine
#28
JOURNAL ARTICLE
Alexander Lee, Sara Weintraub, Ianto Lin Xi, Jaimo Ahn, Joseph Bernstein
BACKGROUND: Displaced femoral neck fractures in geriatric patients are typically treated with either hemiarthroplasty or total hip arthroplasty. The choice between hemiarthroplasty and total hip arthroplasty requires a good estimate of the patient's life expectancy, as the recent HEALTH trial suggests that the benefits of the two operations do not diverge, if at all, until the second year post-operatively. A systematic review was this performed to determine if there sufficient information in the medical literature to estimate a patient's life expectancy beyond two years and to identify those patient variables affecting survival of that duration...
2021: PloS One
#29
REVIEW
Ali S Farooqi, Alexander Lee, David Novikov, Ann Marie Kelly, Xinning Li, John D Kelly, Robert L Parisien
BACKGROUND: With recent improvements in transducer strength, image resolution, and operator training, ultrasound (US) provides an excellent alternative imaging modality for the diagnosis of rotator cuff tears. PURPOSE: To evaluate the diagnostic accuracy of US for partial- and full-thickness rotator cuff tears and biceps tendon tears, compare diagnostic values with those of magnetic resonance imaging (MRI) using arthroscopy as the reference standard, assess longitudinal improvements in accuracy, and compare diagnostic values from operators with different training backgrounds...
October 2021: Orthopaedic Journal of Sports Medicine
#30
JOURNAL ARTICLE
Alexander Lee, Ali S Farooqi, David Novikov, Xinning Li, John D Kelly, Robert L Parisien
BACKGROUND: In recent years, superior capsular reconstruction (SCR) has emerged as a promising treatment for massive rotator cuff tears and has been performed with an array of graft options, most commonly dermal allograft and tensor fascia lata (TFL) autograft. PURPOSE: To compare the clinical outcomes, functional outcomes, and complication rates after SCR performed with dermal allograft, TFL autograft, long head of the biceps tendon (LHBT) autograft, and porcine xenograft...
December 2022: American Journal of Sports Medicine
#31
JOURNAL ARTICLE
Luis F Schachner, Denise P Tran, Alexander Lee, John P McGee, Kevin Jooss, Kenneth Durbin, Henrique Dos Santos Seckler, Lauren Adams, Erika Cline, Rafael Melani, Ashley N Ives, Benjamin Des Soye, Neil L Kelleher, Steven M Patrie
The combined use of electrospray ionization run in so-called "native mode" with top-down mass spectrometry (nTDMS) is enhancing both structural biology and discovery proteomics by providing three levels of information in a single experiment: the intact mass of a protein or complex, the masses of its subunits and non-covalent cofactors, and fragment ion masses from direct dissociation of subunits that capture the primary sequence and combinations of diverse post-translational modifications (PTMs). While intact mass data are readily deconvoluted using well-known software options, the analysis of fragmentation data that result from a tandem MS experiment - essential for proteoform characterization - is not yet standardized...
July 2021: International Journal of Mass Spectrometry
#32
Teressa Reanne Ju, Hsin Tseng, Hsin-Ti Lin, Alexander Lee Wang, Chi Chan Lee, Yi-Ching Lai
BACKGROUND: Temporary transvenous pacing through the coronary sinus is a novel approach rarely used in treating unstable bradycardia. This modality could provide cardiac pacing while achieving better ventricular synchrony. We present a case who received cardiac pacing through the coronary sinus and provide a summary of evidence in the current literature. CASE SUMMARY: A 55-year-old woman with a history of advanced heart failure was admitted to the rehabilitation ward after a recent stroke...
July 16, 2021: World Journal of Clinical Cases
#33
JOURNAL ARTICLE
Katie Kanter, Ryan Gallagher, Feyisope Eweje, Alexander Lee, David Gordon, Stephen Landy, Julia Gasior, Haideliza Soto-Calderon, Peter F Cronholm, Ben Cocchiaro, James Weimer, Alexis Roth, Stephen Lankenau, Jacob Brenner
BACKGROUND: The incidence of opioid-related overdose deaths has been rising for 30 years and has been further exacerbated amidst the COVID-19 pandemic. Naloxone can reverse opioid overdose, lower death rates, and enable a transition to medication for opioid use disorder. Though current formulations for community use of naloxone have been shown to be safe and effective public health interventions, they rely on bystander presence. We sought to understand the preferences and minimum necessary conditions for wearing a device capable of sensing and reversing opioid overdose among people who regularly use opioids...
July 23, 2021: Harm Reduction Journal
#34
JOURNAL ARTICLE
Renelle Myers, Michael Brauer, Trevor Dummer, Sukhinder Atkar-Khattra, John Yee, Barbara Melosky, Cheryl Ho, Anna L McGuire, Sophie Sun, Kyle Grant, Alexander Lee, Martha Lee, Weiran Yuchi, Martin Tammemagi, Stephen Lam
INTRODUCTION: Air pollution may play an important role in the development of lung cancer in people who have never smoked, especially among East Asian women. The aim of this study was to compare cumulative ambient air pollution exposure between ever and never smokers with lung cancer. METHODS: A consecutive case series of never and ever smokers with newly diagnosed lung cancer were compared regarding their sex, race, and outdoor and household air pollution exposure...
November 2021: Journal of Thoracic Oncology
#35
JOURNAL ARTICLE
Avram Flamm, Alexander Lee, Francis Mencl
OBJECTIVE: Coronavirus disease 2019 (COVID-19, caused by severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) is a historic pandemic severely impacting health care. This study examines its early effect on a busy academic emergency department. METHODS: A retrospective analysis of patients from an academic tertiary care Level I trauma, cardiac and stroke center's emergency department seeing an average of 54,000 adults and 21,000 pediatric patients per year...
June 8, 2021: Disaster Medicine and Public Health Preparedness
#36
JOURNAL ARTICLE
Aylin Visram, Meredith Foley, Xavier Alexander Lee
No abstract text is available yet for this article.
April 26, 2021: Journal of Physiology
#37
REVIEW
Patrick B Meagher, Xavier Alexander Lee, Joseph Lee, Aylin Visram, Mark K Friedberg, Kim A Connelly
Cardiac fibrosis is a common finding that is associated with the progression of heart failure (HF) and impacts all chambers of the heart. Despite intense research, the treatment of HF has primarily focused upon strategies to prevent cardiomyocyte remodeling, and there are no targeted antifibrotic strategies available to reverse cardiac fibrosis. Cardiac fibrosis is defined as an accumulation of extracellular matrix (ECM) proteins which stiffen the myocardium resulting in the deterioration cardiac function. This occurs in response to a wide range of mechanical and biochemical signals...
March 31, 2021: Cells
#38
JOURNAL ARTICLE
Shuo Li, Sumana Raychaudhuri, Stephen Alexander Lee, Marisa M Brockmann, Jing Wang, Grant Kusick, Christine Prater, Sarah Syed, Hanieh Falahati, Raul Ramos, Tomas M Bartol, Eric Hosy, Shigeki Watanabe
Neurotransmitter is released synchronously and asynchronously following an action potential. Our recent study indicates that the release sites of these two phases are segregated within an active zone, with asynchronous release sites enriched near the center in mouse hippocampal synapses. Here we demonstrate that synchronous and asynchronous release sites are aligned with AMPA receptor and NMDA receptor clusters, respectively. Computational simulations indicate that this spatial and temporal arrangement of release can lead to maximal membrane depolarization through AMPA receptors, alleviating the pore-blocking magnesium leading to greater activation of NMDA receptors...
January 29, 2021: Nature Communications
#39
JOURNAL ARTICLE
Mitchell Savic, Mohsen Kazemi, Alexander Lee, David Starmer, Sheilah Hogg-Johnson
Objective: To evaluate the test-retest reliability and construct validity of the concussion knowledge assessment tool (CKAT) as a measure of knowledge of concussion and its management among chiropractic subgroups and to compare these properties for two scoring strategies for the CKAT. Methods: Three chiropractic subgroups (first year students, interns and sports chiropractors) completed the CKAT via SurveyMonkey with as second administration two to six weeks later for a subset of respondents...
December 2020: Journal of the Canadian Chiropractic Association
#40
JOURNAL ARTICLE
Edwin S Dalmaijer, Alexander Lee, Rachel Leiter, Zoe Brown, Thomas Armstrong
Disgust is an adaptation forged under the selective pressure of pathogens. Yet disgust may cause problems in contemporary societies because of its propensity for "false positives" and resistance to corrective information. Here, we investigate whether disgust, as revealed by oculomotor avoidance, might be reduced through the noncognitive process of habituation. In each of three experiments, we repeatedly exposed participants to the same pair of images, one disgusting and one neutral, and recorded gaze...
January 21, 2021: Journal of Experimental Psychology. General
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