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Alex lee

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https://www.readbyqxmd.com/read/27898091/restoring-ureagenesis-in-hepatocytes-by-crispr-cas9-mediated-genomic-addition-to-arginase-deficient-induced-pluripotent-stem-cells
#1
Patrick C Lee, Brian Truong, Agustin Vega-Crespo, W Blake Gilmore, Kip Hermann, Stephanie Ak Angarita, Jonathan K Tang, Katherine M Chang, Austin E Wininger, Alex K Lam, Benjamen E Schoenberg, Stephen D Cederbaum, April D Pyle, James A Byrne, Gerald S Lipshutz
Urea cycle disorders are incurable enzymopathies that affect nitrogen metabolism and typically lead to hyperammonemia. Arginase deficiency results from a mutation in Arg1, the enzyme regulating the final step of ureagenesis and typically results in developmental disabilities, seizures, spastic diplegia, and sometimes death. Current medical treatments for urea cycle disorders are only marginally effective, and for proximal disorders, liver transplantation is effective but limited by graft availability. Advances in human induced pluripotent stem cell research has allowed for the genetic modification of stem cells for potential cellular replacement therapies...
November 29, 2016: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/27883890/a-consensus-genome-scale-reconstruction-of-chinese-hamster-ovary-cell-metabolism
#2
Hooman Hefzi, Kok Siong Ang, Michael Hanscho, Aarash Bordbar, David Ruckerbauer, Meiyappan Lakshmanan, Camila A Orellana, Deniz Baycin-Hizal, Yingxiang Huang, Daniel Ley, Veronica S Martinez, Sarantos Kyriakopoulos, Natalia E Jiménez, Daniel C Zielinski, Lake-Ee Quek, Tune Wulff, Johnny Arnsdorf, Shangzhong Li, Jae Seong Lee, Giuseppe Paglia, Nicolas Loira, Philipp N Spahn, Lasse E Pedersen, Jahir M Gutierrez, Zachary A King, Anne Mathilde Lund, Harish Nagarajan, Alex Thomas, Alyaa M Abdel-Haleem, Juergen Zanghellini, Helene F Kildegaard, Bjørn G Voldborg, Ziomara P Gerdtzen, Michael J Betenbaugh, Bernhard O Palsson, Mikael R Andersen, Lars K Nielsen, Nicole Borth, Dong-Yup Lee, Nathan E Lewis
Chinese hamster ovary (CHO) cells dominate biotherapeutic protein production and are widely used in mammalian cell line engineering research. To elucidate metabolic bottlenecks in protein production and to guide cell engineering and bioprocess optimization, we reconstructed the metabolic pathways in CHO and associated them with >1,700 genes in the Cricetulus griseus genome. The genome-scale metabolic model based on this reconstruction, iCHO1766, and cell-line-specific models for CHO-K1, CHO-S, and CHO-DG44 cells provide the biochemical basis of growth and recombinant protein production...
November 23, 2016: Cell Systems
https://www.readbyqxmd.com/read/27881816/hypothermia-treated-neonates-with-hypoxic-ischemic-encephalopathy-optimal-timing-of-quantitative-adc-measurement-to-predict-disease-severity
#3
Yauk K Lee, Alex Penn, Mahesh Patel, Rajul Pandit, Dongli Song, Bo Yoon Ha
To determine the optimal time window for MR imaging with quantitative ADC measurement in neonatal HIE after hypothermia treatment, a retrospective review was performed on consecutive hypothermia-treated term neonates with HIE, with an initial and follow-up MR imaging within the first two weeks of life. Three neuroradiologists categorized each set of MR imaging as normal, mild, moderate or severe HIE based on a consensus review of the serial imaging. The lowest ADC values from the white matter, corpus callosum, and basal ganglia/thalamus were measured...
November 23, 2016: Neuroradiology Journal
https://www.readbyqxmd.com/read/27878739/divergence-of-age-related-differences-in-social-communication-improvements-for-typically-developing-youth-but-declines-for-youth-with-autism-spectrum-disorder
#4
Gregory L Wallace, Katerina Dudley, Laura Anthony, Cara E Pugliese, Bako Orionzi, Liv Clasen, Nancy Raitano Lee, Jay N Giedd, Alex Martin, Armin Raznahan, Lauren Kenworthy
Although social-communication difficulties and repetitive behaviors are hallmark features of autism spectrum disorder (ASD) and persist across the lifespan, very few studies have compared age-related differences in these behaviors between youth with ASD and same-age typically developing (TD) peers. We examined this issue using SRS-2 (Social Responsiveness Scale-Second Edition) measures of social-communicative functioning and repetitive behaviors in a stratified cross-sectional sample of 324 youth with ASD in the absence of intellectual disability, and 438 TD youth (aged 4-29 years)...
November 23, 2016: Journal of Autism and Developmental Disorders
https://www.readbyqxmd.com/read/27872126/multiple-ligament-reconstruction-femoral-tunnels-intertunnel-relationships-and-guidelines-to-avoid-convergence
#5
Gilbert Moatshe, Alex W Brady, Erik L Slette, Jorge Chahla, Travis Lee Turnbull, Lars Engebretsen, Robert F LaPrade
BACKGROUND: Knee dislocations often require multiple concurrent ligament reconstructions, which involve creating several tunnels in the distal femur. Therefore, the risk of tunnel convergence is increased because of the limited bone volume within the distal aspect of the femur. PURPOSE: To assess the risk of tunnel convergence and determine the optimal reconstruction tunnel orientations for multiple ligament reconstructions in the femur. STUDY DESIGN: Descriptive laboratory study...
November 21, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27870970/panoramic-characterization-of-endocardial-left-atrial-activation-during-human-persistent-af-insights-from-non-contact-mapping
#6
Geoffrey Lee, Alex J A McLellan, Ross J Hunter, Matthew J Lovell, Malcolm Finlay, Waqas Ullah, Mehul B Dhinoja, Simon Sporton, Mark J Earley, Richard J Schilling
BACKGROUND: The relative importance of focal drivers, multiple wavelets, rotors and endocardial-epicardial circuits in the maintenance of persistent AF remains unclear. Our objective was to characterize AF wavefront (WF) dynamics during persistent AF. METHODS: The Ensite 3000 (St Jude Medical) non-contact mapping system was used to map the LA of 15 patients with persistent AF. Wavefronts were classified into planar WFs, rotors or focal WFs. For each new WF the site of origin, the unipolar electrogram, and propagation patterns were determined...
November 9, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27870739/performance-of-the-angle-labor-pain-questionnaire-during-initiation-of-epidural-analgesia-in-early-active-labor
#7
Pamela J Angle, Christine Kurtz Landy, Jasmine Djordjevic, Jon Barrett, Alanna Kibbe, Saiena Sriparamananthan, Yuna Lee, Lydia Hamata, Pearl Zaki, Alex Kiss
BACKGROUND: The Angle Labor Pain Questionnaire (A-LPQ) is a new, 22-item multidimensional psychometric questionnaire that measures the 5 most important dimensions of women's childbirth pain experiences using 5 subscales: The Enormity of the Pain, Fear/Anxiety, Uterine Contraction Pain, Birthing Pain, and Back Pain/Long Haul. Previous work showed that the A-LPQ has overall good psychometric properties and performance during early active labor in women without pain relief. The current study assessed the tool's sensitivity to change during initiation of labor epidural analgesia with the standardized response mean (SRM, primary outcome)...
December 2016: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/27832824/genome-of-the-asian-longhorned-beetle-anoplophora-glabripennis-a-globally-significant-invasive-species-reveals-key-functional-and-evolutionary-innovations-at-the-beetle-plant-interface
#8
Duane D McKenna, Erin D Scully, Yannick Pauchet, Kelli Hoover, Roy Kirsch, Scott M Geib, Robert F Mitchell, Robert M Waterhouse, Seung-Joon Ahn, Deanna Arsala, Joshua B Benoit, Heath Blackmon, Tiffany Bledsoe, Julia H Bowsher, André Busch, Bernarda Calla, Hsu Chao, Anna K Childers, Christopher Childers, Dave J Clarke, Lorna Cohen, Jeffery P Demuth, Huyen Dinh, HarshaVardhan Doddapaneni, Amanda Dolan, Jian J Duan, Shannon Dugan, Markus Friedrich, Karl M Glastad, Michael A D Goodisman, Stephanie Haddad, Yi Han, Daniel S T Hughes, Panagiotis Ioannidis, J Spencer Johnston, Jeffery W Jones, Leslie A Kuhn, David R Lance, Chien-Yueh Lee, Sandra L Lee, Han Lin, Jeremy A Lynch, Armin P Moczek, Shwetha C Murali, Donna M Muzny, David R Nelson, Subba R Palli, Kristen A Panfilio, Dan Pers, Monica F Poelchau, Honghu Quan, Jiaxin Qu, Ann M Ray, Joseph P Rinehart, Hugh M Robertson, Richard Roehrdanz, Andrew J Rosendale, Seunggwan Shin, Christian Silva, Alex S Torson, Iris M Vargas Jentzsch, John H Werren, Kim C Worley, George Yocum, Evgeny M Zdobnov, Richard A Gibbs, Stephen Richards
BACKGROUND: Relatively little is known about the genomic basis and evolution of wood-feeding in beetles. We undertook genome sequencing and annotation, gene expression assays, studies of plant cell wall degrading enzymes, and other functional and comparative studies of the Asian longhorned beetle, Anoplophora glabripennis, a globally significant invasive species capable of inflicting severe feeding damage on many important tree species. Complementary studies of genes encoding enzymes involved in digestion of woody plant tissues or detoxification of plant allelochemicals were undertaken with the genomes of 14 additional insects, including the newly sequenced emerald ash borer and bull-headed dung beetle...
November 11, 2016: Genome Biology
https://www.readbyqxmd.com/read/27819188/feasibility-of-3-d-bioprinting-with-a-modified-desktop-3d-desktop-printer
#9
Todd Goldstein, Lee P Smith, Alex Krush, Kevin Mercadante, Dan Lagalante, Casey Epstein, John A Schwartz, David Zeltsman, Daniel Anthony Grande
Numerous studies have shown the capabilities of 3D printing for use in the medical industry. At the time of this publication, basic home desktop 3-D printer kits can cost as little as $300, while medical-specific 3D bioprinters can cost upwards of $300,000. The purpose of this study was to show how a commercially available desktop 3D printer could be modified to bioprint an engineered poly-L-lactic acid (PLLA) scaffold containing viable chondrocytes in a bioink. Our bioprinter was used to create a living 3D functional tissue-engineered cartilage scaffold...
November 7, 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27816520/inherently-variable-responses-to-glucocorticoid-stress-among-endogenous-retroviruses-isolated-from-23-mouse-strains
#10
Karen Hsu, Young-Kwan Lee, Alex Chew, Sophia Chiu, Debora Lim, David G Greenhalgh, Kiho Cho
Active participation of endogenous retroviruses (ERVs) in disease processes has been exemplified by the finding that the HERV (human ERV)-W envelope protein is involved in the pathogenesis of multiple sclerosis, an autoimmune disease. We also demonstrated that injury-elicited stressors alter the expression of murine ERVs (MuERVs), both murine leukemia virus-type and mouse mammary tumor virus (MMTV)-type (MMTV-MuERV). In this study, to evaluate MMTV-MuERVs' responses to stress (e.g., injury, infection)-elicited systemic glucocorticoid (GC) levels, we examined the GC-stress response of 64 MMTV-MuERV promoters isolated from the genomes of 23 mouse strains...
November 3, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27811116/draft-genome-sequence-of-piscirickettsia-litoralis-isolated-from-seawater
#11
Xuehua Wan, Alex J Lee, Shaobin Hou, Blake Ushijima, Yen P Nguyen, Jessica A Thawley, Stuart P Donachie
One species of Piscirickettsia, a pathogen of salmonid fish, has been described. The genome sequence of a putative second and free-living species may provide insights into the evolution of pathogenicity in the genus.
November 3, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27808130/volumetric-mapping-of-barrett-s-esophagus-and-dysplasia-with-en-face-optical-coherence-tomography-tethered-capsule
#12
Kaicheng Liang, Osman O Ahsen, Hsiang-Chieh Lee, Zhao Wang, Benjamin M Potsaid, Marisa Figueiredo, Vijaysekhar Jayaraman, Alex E Cable, Qin Huang, Hiroshi Mashimo, James G Fujimoto
No abstract text is available yet for this article.
November 2016: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/27805565/computationally-designed-high-specificity-inhibitors-delineate-the-roles-of-bcl2-family-proteins-in-cancer
#13
Stephanie Berger, Erik Procko, Daciana Margineantu, Erinna F Lee, Betty W Shen, Alex Zelter, Daniel-Adriano Silva, Kusum Chawla, Marco J Herold, Jean-Marc Garnier, Richard Johnson, Michael J MacCoss, Guillaume Lessene, Trisha N Davis, Patrick S Stayton, Barry L Stoddard, W Douglas Fairlie, David M Hockenbery, David Baker
Many cancers overexpress one or more of the six human pro-survival BCL2 family proteins to evade apoptosis. To determine which BCL2 protein or proteins block apoptosis in different cancers, we computationally designed three-helix bundle protein inhibitors specific for each BCL2 pro-survival protein. Following in vitro optimization, each inhibitor binds its target with high picomolar to low nanomolar affinity and at least 300-fold specificity. Expression of the designed inhibitors in human cancer cell lines revealed unique dependencies on BCL2 proteins for survival which could not be inferred from other BCL2 profiling methods...
November 2, 2016: ELife
https://www.readbyqxmd.com/read/27782289/unobtrusive-and-continuous-monitoring-of-alcohol-impaired-gait-using-smart-shoes
#14
Eunjeong Park, Sunghoon I Lee, Hyo Suk Nam, Jordan H Garst, Alex Huang, Andrew Campion, Monica Arnell, Nima Ghalehsariand, Sangsoo Park, Hyuk-Jae Chang, Daniel C Lu, Majid Sarrafzadeh
BACKGROUND: Alcohol ingestion influences sensory-motor function and the overall well-being of individuals. Detecting alcohol-induced impairments in gait in daily life necessitates a continuous and unobtrusive gait monitoring system. OBJECTIVES: This paper introduces the development and use of a non-intrusive monitoring system to detect changes in gait induced by alcohol intoxication. METHODS: The proposed system employed a pair of sensorized smart shoes that are equipped with pressure sensors on the insole...
October 26, 2016: Methods of Information in Medicine
https://www.readbyqxmd.com/read/27771548/stepwise-thinning-dynamics-of-a-foam-film-formed-from-an-anionic-micellar-solution
#15
Jongju Lee, Alex Nikolov, Darsh Wasan
Liquid films formed from a surfactant micellar solution thin in a stepwise fashion (stratify): the film thickness decreases layer-by-layer and dark spots appear (areas of the film with one micellar layer less than their surroundings). In a recent paper [Langmuir2016, 32, 4837-4847], we presented a two-dimensional diffusion model to explain the expansion of the dark spots inside the foam film formed from a nonionic surfactant micellar solution. Here, we apply the model to explain the dark spot expansion in the foam film formed from an anionic surfactant micellar solution of sodium dodecyl sulfate...
October 19, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27769165/distinct-gene-expression-program-dynamics-during-erythropoiesis-from-human-induced-pluripotent-stem-cells-compared-with-adult-and-cord-blood-progenitors
#16
Alison T Merryweather-Clarke, Alex J Tipping, Abigail A Lamikanra, Rui Fa, Basel Abu-Jamous, Hoi Pat Tsang, Lee Carpenter, Kathryn J H Robson, Asoke K Nandi, David J Roberts
BACKGROUND: Human-induced pluripotent stem cells (hiPSCs) are a potentially invaluable resource for regenerative medicine, including the in vitro manufacture of blood products. HiPSC-derived red blood cells are an attractive therapeutic option in hematology, yet exhibit unexplained proliferation and enucleation defects that presently preclude such applications. We hypothesised that substantial differential regulation of gene expression during erythroid development accounts for these important differences between hiPSC-derived cells and those from adult or cord-blood progenitors...
October 21, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27765847/dedifferentiation-of-glioma-cells-to-glioma-stem-like-cells-by-therapeutic-stress-induced-hif-signaling-in-the-recurrent-gbm-model
#17
Gina Lee, Brenda Auffinger, Donna Guo, Tanwir Hasan, Marc Deheeger, Alex L Tobias, Jeong Yeon Kim, Fatemeh Atashi, Lingjiao Zhang, Maciej S Lesniak, James C David, Atique U Ahmed
Increasing evidence exposes a subpopulation of cancer cells, known as cancer stem cells (CSCs), to be critical for the progression of several human malignancies, including glioblastoma multiforme. CSCs are highly tumorigenic, capable of self-renewal, and resistant to conventional therapies, and thus considered to be one of the key contributors to disease recurrence. To elucidate the poorly understood evolutionary path of tumor recurrence and the role of CSCs in this process, we developed patient-derived xenograft glioblastoma recurrent models induced by anti-glioma chemotherapy, temozolomide...
December 2016: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/27759898/determining-the-optimal-dose-of-adenosine-for-unmasking-dormant-pulmonary-vein-conduction-following-atrial-fibrillation-ablation-electrophysiological-and-hemodynamic-assessment-dormant-af-study
#18
Sandeep Prabhu, Vincent Mackin, Alex Ja McLellan, Tuong Phan, Desmond McGlade, Liang-Han Ling, Kah Y Peck, Alexandr Voskoboinik, Bupesh Pathik, Chrishan J Nalliah, Geoff R Wong, Sonia M Azzopardi, Geoffrey Lee, Justin Mariani, Andrew J Taylor, Jonathan M Kalman, Peter M Kistler
INTRODUCTION: The significance of adenosine induced dormant pulmonary vein (PV) conduction in AF ablation remains controversial. The optimal dose of adenosine to determine dormant PV conduction is yet to be systematically explored. METHODS AND RESULTS: Consecutive patients undergoing index AF ablation received 3 adenosine doses (12mg, 18mg, 24mg) in a randomized blinded order, immediately after PVI. Electrophysiological (PR prolongation, AV block (AVB) and PV reconnection) and hemodynamic (BP) parameters were measured...
October 19, 2016: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/27754266/os-37-09-layer-specific-quantification-of-myocardial-deformation-may-disclose-the-subclinical-systolic-dysfunction-and-the-mechanism-of-reserved-ejection-fraction-in-patients-with-hypertension
#19
Tingyan Xu, Jingping Sun, Alex Pui-Wai Lee, Yan Yang, Xingsheng Yang, Yan Li, Cheuk-Man Yu, Jiguang Wang
OBJECTIVE: Hypertension (HTN) leads to left ventricular (LV) remodeling, which results in diastolic dysfunction in the presence of preserved ejection fraction (EF). The goal of this study was to explore the subclinical left ventricular systolic dysfunction and mechanism of preserved EF using layer-specific quantification of myocardial deformation in patients with HTN. DESIGN AND METHOD: The routine 2D (frame rate >50 Hz) data sets were acquired using GE E9 system in 120 hypertensive patients (73 male, 54 ± 13years, blood pressure = 165 ± 20/ 96 ± 117 mmHg) and 120 age and gender matched normal subjects (124 ± 9/80 ± 8mmHg)...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27741177/antenatal-corticosteroids-and-outcomes-of-small-for-gestational-age-neonates
#20
Nir Melamed, Alex Pittini, Jon Barrett, Jyotsna Shah, Eugene W Yoon, Brigitte Lemyre, Shoo K Lee, Kellie E Murphy, Prakesh S Shah
OBJECTIVE: To assess the association of antenatal corticosteroids and neonatal outcomes of preterm small-for-gestational-age (SGA) neonates and estimate whether the association is similar to that observed in appropriate-for-gestational-age (AGA) neonates. METHODS: We conducted a retrospective cohort study using data collected on singleton neonates born between 24 0/7 and 33 6/7 weeks of gestation and admitted to tertiary neonatal units in Canada between 2010 and 2014...
November 2016: Obstetrics and Gynecology
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