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https://www.readbyqxmd.com/read/28919950/chromosome-mapping-of-a-tc1-like-transposon-in-species-of-the-catfish-ancistrus
#1
Keteryne Rodrigues da Silva, Sandra Mariotto, Liano Centofante, Patricia Pasquali Parise-Maltempi
The Tc1 mariner element is widely distributed among organisms and have been already described in different species of fish. The genus Ancistrus (Kner, 1854) has 68 nominal species and is part of an interesting taxonomic and cytogenetic group, as well as presenting a variation of chromosome number, ranging from 2n=34 to 54 chromosomes, and the existence of simple and multiple sex chromosome system and the occurrence of chromosomal polymorphisms involving chromosomes that carry the nucleolus organizer region...
2017: Comparative Cytogenetics
https://www.readbyqxmd.com/read/28919949/first-report-of-b-chromosomes-in-three-neotropical-thorny-catfishes-siluriformes-doradidae
#2
Fábio Hiroshi Takagui, Ana Lucia Dias, José Luís Olivan Birindelli, Ana Claudia Swarça, Renata da Rosa, Roberto Laridondo Lui, Alberto Sergio Fenocchio, Lucia Giuliano-Caetano
The family Doradidae (Siluriformes) is an important group of fishes endemic to freshwater ecosystems in South America. Some cytogenetic studies have been conducted focused on the group; however, there are no reports on the occurrence of B chromosomes for the family. In this paper the chromosomal characteristics of Platydoras armatulus (Valenciennes, 1840), Pterodoras granulosus (Valenciennes, 1821) and Ossancora punctata (Kner, 1855) were investigated through classical cytogenetics approaches. The conventional staining reveals 2n=58 in Platydoras armatulus and Pterodoras granulosus, however with distinct karyotypic formulae, possibly originated by pericentric inversions...
2017: Comparative Cytogenetics
https://www.readbyqxmd.com/read/28693645/morphometrics-and-allometry-of-the-larvae-of-five-characiformes-species-in-the-para%C3%A3-ba-do-sul-river-basin
#3
Guilherme Souza, Erica P Caramaschi, Leandro R Monteiro
The aim of this study was to analyse the morphology and allometry of larvae belonging to five potamodromous species. Five breeding species belonging to the order Characiformes [Salminus brasiliensis (Cuvier, 1816), Leporinus steindachneri, Eigenmann, 1907, Prochilodus lineatus (Valenciennes, 1837), Prochilodus vimboides (Kner,1859) and Brycon insignis, Steindachner, 1877] were used to obtain larvae samples during the pre-flexing, post-flexing, and juvenile developmental stages. When we observed the degree-hour (DH) amplitude time values, we found three developmental groups based on allometry and morphometrics within the period between the pre-flexing and post-flexing phases...
July 11, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28562242/ift-trains-in-different-stages-of-assembly-queue-at-the-ciliary-base-for-consecutive-release-into-the-cilium
#4
Jenna L Wingfield, Ilaria Mengoni, Heather Bomberger, Yu-Yang Jiang, Jonathon D Walsh, Jason M Brown, Tyler Picariello, Deborah A Cochran, Bing Zhu, Junmin Pan, Jonathan Eggenschwiler, Jacek Gaertig, George B Witman, Peter Kner, Karl Lechtreck
Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic proteins in cilia. To study how trains assemble, we employed fluorescence protein-tagged IFT proteins in Chlamydomonas reinhardtii. IFT-A and motor proteins are recruited from the cell body to the basal body pool, assembled into trains, move through the cilium, and disperse back into the cell body. In contrast to this 'open' system, IFT-B proteins from retrograde trains reenter the pool and a portion is reused directly in anterograde trains indicating a 'semi-open' system...
May 31, 2017: ELife
https://www.readbyqxmd.com/read/28485143/charge-directed-immobilization-of-bacteriophage-on-nanostructured-electrode-for-whole-cell-electrochemical-biosensors
#5
Yan Zhou, Abhijit Marar, Peter Kner, Ramaraja P Ramasamy
A new type of carbon nanotube (CNT)-based impedimetric biosensing method has been developed for rapid and selective detection of live bacterial cells. A proof-of-concept study was conducted using T2 bacteriophage-based biosensors for electrochemical detection of Escherichia coli B. The T2 bacteriophage (virus) served as the biorecognition element, which was immobilized on polyethylenimine (PEI)-functionalized carbon nanotube transducer on glassy carbon electrode. Charge-directed, orientated immobilization of bacteriophage particles on carbon nanotubes was achieved through covalent linkage of phage capsid onto the carbon nanotubes...
June 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28323304/characterization-of-wavefront-errors-in-mouse-cranial-bone-using-second-harmonic-generation
#6
Kayvan Forouhesh Tehrani, Peter Kner, Luke J Mortensen
No abstract text is available yet for this article.
March 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28199937/semiquantitative-determination-of-total-mercury-in-pygocentrus-nattereri-kner-1858-and-sediment-at-the-plateau-of-upper-paraguai-river-brazil
#7
Clautenes Maria de Almeida Ferreira, Silvia Gonçalves Egler, Allegra Viviane Yallouz, Áurea Regina Alves Ignácio
In this study an environmental assessment of contamination by total mercury (THg) was carried out at the Plateau of the Upper Paraguai River. Total mercury was evaluated in sediment and muscle of the red piranha Pygocentrus nattereri Kner, 1858, a piscivorous species at the top of the food chain consumed for subsistence and commercially. THg concentrations were below national guidelines established by WHO for sediments (100 ng g(-1)) and fish (100-600 ng g(-1)) for most of the sampled sites. Two sites located downstream of artisanal diamond and gold mines had THg concentrations in fish equal or greater than 600 ng g(-1)...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/27516875/genetic-differentiation-in-red-bellied-piranha-populations-pygocentrus-nattereri-kner-1858-from-the-solim%C3%A3%C2%B5es-amazonas-river
#8
Carlos Henrique Dos A Dos Santos, Carolina S de Sá Leitão, Maria de N Paula-Silva, Vera Maria F Almeida-Val
Red-bellied piranhas (Pygocentrus nattereri) are widely caught with different intensities throughout the region of Solimões-Amazonas River by local fishermen. Thus, the management of this resource is performed in the absence of any information on its genetic stock. P. nattereri is a voracious predator and widely distributed in the Neotropical region, and it is found in other regions of American continent. However, information about genetic variability and structure of wild populations of red-bellied piranha is unavailable...
June 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/27394824/reconciling-more-than-150-years-of-taxonomic-confusion-%C3%A2-the-true-identity-of-moenkhausia-lepidura-with%C3%A2-a-key-to-the-species-of-the%C3%A2-m-lepidura%C3%A2-group-characiformes-characidae
#9
Manoela M F Marinho, Francisco Langeani
Moenkhausia lepidura (Kner) is redescribed based on the examination of the type and other specimens from several localities of the Amazon and Orinoco basins. The species is readly diagnosed from congeners by a combination of a dark blotch on the upper caudal-fin lobe, lower lobe hyaline, and by the arrangement of predorsal scales, in which scales of anterior portion of predorsal area are arranged in pairs, followed by a single median row of scales extending to dorsal-fin origin. The analysis of the type material of Gymnotichthys hildae Fernández-Yépez and Knodus calliurus Ahl demonstrated the former is a junior synonym of Moenkhausia lepidura, whereas the latter should be removed from its synonymy...
May 3, 2016: Zootaxa
https://www.readbyqxmd.com/read/26831769/interferometric-imaging-with-three-objectives
#10
Thomas Huelsnitz, Peter Kner
We propose a microscope that utilizes three high numerical aperture objectives to image a sample from multiple angles, interferometrically combining the fields from each objective to obtain a higher resolution and more isotropic point-spread function than standard wide-field and confocal techniques. The proposed microscope utilizes three 0.9 NA objectives with large working distance and, therefore, has both a large imaging volume and an effective 2.7 NA. The FWHM of the point-spread function is 135 nm in the plane of the objectives, a factor of 2 better than the lateral resolution with a single objective...
February 1, 2016: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/26631555/single-particle-imaging-reveals-intraflagellar-transport-independent-transport-and-accumulation-of-eb1-in-chlamydomonas-flagella
#11
J Aaron Harris, Yi Liu, Pinfen Yang, Peter Kner, Karl F Lechtreck
The microtubule (MT) plus-end tracking protein EB1 is present at the tips of cilia and flagella; end-binding protein 1 (EB1) remains at the tip during flagellar shortening and in the absence of intraflagellar transport (IFT), the predominant protein transport system in flagella. To investigate how EB1 accumulates at the flagellar tip, we used in vivo imaging of fluorescent protein-tagged EB1 (EB1-FP) in Chlamydomonas reinhardtii. After photobleaching, the EB1 signal at the flagellar tip recovered within minutes, indicating an exchange with unbleached EB1 entering the flagella from the cell body...
January 15, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/26560663/genetic-differentiation-in-the-populations-of-red-piranha-pygocentrus-nattereri-kner-1860-characiformes-serrasalminae-from-the-river-basins-of-northeastern-brazil
#12
L A Luz, L L Reis, I Sampaio, M C Barros, E Fraga
AbstractThe red piranha, Pygocentrus nattereri, is an important resource for artisanal and commercial fisheries. The present study determines the genetic differentiation among P. nattereri populations from the northeastern Brazilian state of Maranhão. The DNA was isolated using a standard phenol-chloroform protocol and the Control Region was amplified by PCR. The PCR products were sequenced using the didesoxyterminal method. A sequence of 1039 bps was obtained from the Control Region of 60 specimens, which presented 33 polymorphic sites, 41 haplotypes, һ =0...
November 10, 2015: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/26287090/trans-andean-ancistrus-siluriformes-loricariidae
#13
Donald C Taphorn, Jonathan W Armbruster, Francisco Villa-Navarro, C Keith Ray
We review the trans-Andean species of Ancistrus from Panama, Colombia and Venezuela. Based on analyses of meristic, morphometric and pigmentation pattern data of preserved specimens, eight of sixteen species reported from this region are considered valid and two new species are described. Here we review Ancistrus chagresi Eigenmann & Eigenmann 1889 from both slopes of central Panama; A. centrolepis Regan 1913 from Pacific slopes of eastern Panama and western Colombia; Ancistrus caucanus Fowler 1943, from the Magdalena River drainage in northern Colombia; Ancistrus martini Schultz 1944, from the Lake Maracaibo Basin of Venezuela and Colombia...
2013: Zootaxa
https://www.readbyqxmd.com/read/26258516/complete-mitochondrial-genome-of-rhodeus-ocellatus-cypriniformes-cyprinidae
#14
Juan Hu, Yibin Chen, Huihong Zhao, Huirong Yang, Li Liu
The complete mitogenome sequence of Rhodeus ocellatus (Kner) was determined using the next-generation sequencing (NGS). The genome was 16 761 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a control region. The overall nucleotide composition was 30.43% A, 27.50% T, 25.94% C, and 16.13% G, with an A + T bias of 57.93%. The gene composition and the arrangement of the R. ocellatus mitochondrial genome were similar to that of most other vertebrates...
September 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/26199146/early-development-and-allometric-growth-patterns-of-the-grumat%C3%A3-prochilodus-vimboides-kner-1859
#15
Guilherme Souza, Edésio J T Melo, Erica P Caramaschi, Dalcio R Andrade, Leandro R Monteiro
The objective of this study was to characterize the early development and allometric growth of the grumatã (Prochilodus vimboides). We describe a sample of 266 eggs and larvae obtained through induced spawning. The eggs were spherical (mean 3.7 mm diameter), exhibited a yellow yolk and were non-adhesive and pelagic after fertilization and hydration. The time elapsed between the early cleavage and post-flexion stages was considered short (328 hours, 8054 hour-degrees) in regard to the development times of other Neotropical rheophilic species, but time to hatching was considerably longer than in other Prochilodus species...
June 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/26074617/adaptive-optics-stochastic-optical-reconstruction-microscopy-ao-storm-using-a-genetic-algorithm
#16
Kayvan F Tehrani, Jianquan Xu, Yiwen Zhang, Ping Shen, Peter Kner
The resolution of Single Molecule Localization Microscopy (SML) is dependent on the width of the Point Spread Function (PSF) and the number of photons collected. However, biological samples tend to degrade the shape of the PSF due to the heterogeneity of the index of refraction. In addition, there are aberrations caused by imperfections in the optical components and alignment, and the refractive index mismatch between the coverslip and the sample, all of which directly reduce the accuracy of SML. Adaptive Optics (AO) can play a critical role in compensating for aberrations in order to increase the resolution...
May 18, 2015: Optics Express
https://www.readbyqxmd.com/read/25714992/enhanced-resolution-through-thick-tissue-with-structured-illumination-and-adaptive-optics
#17
Benjamin Thomas, Adrian Wolstenholme, Snehal N Chaudhari, Edward T Kipreos, Peter Kner
Structured illumination microscopy provides twice the linear resolution of conventional fluorescence microscopy, but in thick samples, aberrations degrade the performance and limit the resolution. Here, we demonstrate structured illumination microscopy through 35 μm of tissue using adaptive optics (AO) to correct aberrations resulting in images with a resolution of 140 nm. We report a 60% minimum improvement in the signal-to-noise ratio of the structured illumination reconstruction through thick tissue by correction with AO...
February 2015: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/25703291/multicolor-3d-super-resolution-imaging-by-quantum-dot-stochastic-optical-reconstruction-microscopy
#18
Jianquan Xu, Kayvan F Tehrani, Peter Kner
We demonstrate multicolor three-dimensional super-resolution imaging with quantum dots (QSTORM). By combining quantum dot asynchronous spectral blueing with stochastic optical reconstruction microscopy and adaptive optics, we achieve three-dimensional imaging with 24 nm lateral and 37 nm axial resolution. By pairing two short-pass filters with two appropriate quantum dots, we are able to image single blueing quantum dots on two channels simultaneously, enabling multicolor imaging with high photon counts.
March 24, 2015: ACS Nano
https://www.readbyqxmd.com/read/25583998/tubulin-transport-by-ift-is-upregulated-during-ciliary-growth-by-a-cilium-autonomous-mechanism
#19
Julie M Craft, J Aaron Harris, Sebastian Hyman, Peter Kner, Karl F Lechtreck
The assembly of the axoneme, the structural scaffold of cilia and flagella, requires translocation of a vast quantity of tubulin into the growing cilium, but the mechanisms that regulate the targeting, quantity, and timing of tubulin transport are largely unknown. In Chlamydomonas, GFP-tagged α-tubulin enters cilia as an intraflagellar transport (IFT) cargo and by diffusion. IFT-based transport of GFP-tubulin is elevated in growing cilia and IFT trains carry more tubulin. Cells possessing both nongrowing and growing cilia selectively target GFP-tubulin into the latter...
January 19, 2015: Journal of Cell Biology
https://www.readbyqxmd.com/read/25311466/prolonging-hypothermic-storage-4-c-of-bovine-embryos-with-fish-antifreeze-protein
#20
Atsushi Ideta, Yoshito Aoyagi, Kanami Tsuchiya, Yuuki Nakamura, Kou Hayama, Atsushi Shirasawa, Kenichiro Sakaguchi, Naomi Tominaga, Yoshiyuki Nishimiya, Sakae Tsuda
Embryos obtained via superovulation are necessary for mammalian artificial reproduction, and viability is a key determinant of success. Nonfreezing storage at 4 C is possible, but currently used storage solutions can maintain embryo viability for only 24-48 h. Here we found that 10 mg/ml antifreeze protein (AFP) dissolved in culture medium 199 with 20% (v/v) fetal bovine serum and 25 mM HEPES could keep bovine embryos alive for 10 days at 4 C. We used a recombinant AFP isolated from the notched-fin eelpout (Zoarces elongatus Kner)...
2015: Journal of Reproduction and Development
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