Haiyang Xie, Wenke Guo, Hui Jiang, Ting Zhang, Lei Zhao, Jinjuan Hu, Shuxin Gao, Sunfengda Song, Jiasu Xu, Li Xu, Xinyi Sun, Yi Ding, Lai Jiang, Xianting Ding
Single cell western blot (scWB) is one of the most important methods for cellular heterogeneity profiling. However, current scWB based on conventional photoactive polyacrylamide hydrogel material suffers from the tradeoff between in-gel probing and separation resolution. Here, a highly sensitive temperature-controlled single-cell western blotting (tc-scWB) method is introduced, which is based on a thermo/photo-dualistic-sensitive polyacrylamide hydrogel, namely acrylic acid-functionalized graphene oxide (AFGO) assisted, N-isopropylacrylamide modified polyacrylamide (ANP) hydrogel...
March 14, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)