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thermo activated

José Ignacio López-Sánchez, P A Hancock
There is little doubt that increases in thermal load beyond the thermo-neutral state prove progressively stressful to all living organisms. Increasing temperatures across the globe represent in some locales, and especially for outdoors workers, a significant source of such chronic load increase. However, increases in thermal load affect cognition as well as physical work activities. Such human cognition has perennially been viewed as the primary conduit through which to solve many of the iatrogenic challenges we now face...
June 20, 2017: International Journal of Hyperthermia
Masashi Maruyama, Keisuke Shibuya
Thermo-responsive adsorbents for immunoglobulin G (IgG) employing ε-polylysine (EPL) as a polymer backbone were developed. The introduction of mercaptoethylpyridine (MEP) as an IgG-binding ligand and hydrophobization of side chains afforded thermo-responsive IgG adsorbents, whose thermo-responsive IgG desorption ratio was up to 88% (EPL/MEP derivative 3m). The changes in surface densities of active MEP groups, which are caused by thermal conformational changes of the adsorbents, play key roles for IgG desorption...
June 20, 2017: Biomaterials Science
Sunil Kumar, Shikha Singh, Sudipta Senapati, Akhand Pratap Singh, Biswajit Ray, Pralay Maiti
Biodegradation rate of poly(lactic acid) (PLA) has been regulated, both increase and decrease with respect to the biodegradation of pure PLA, by embedding meager amount of inorganic salts in polymer matrix. Biodegradation is performed in enzyme medium on suspension and film and the extent of biodegradation is measured through spectroscopic technique which is also verified by weight loss measurement. Media pH has been controlled using trace amount of inorganic salt which eventually control the biodegradation of PLA...
June 15, 2017: International Journal of Biological Macromolecules
Nabil Smichi, Houcemeddine Othman, Neila Achouri, Alexandre Noiriel, Soumaya Triki, Vincent Arondel, Najet Srairi-Abid, Abdelkarim Abousalham, Youssef Gargouri, Nabil Miled, Ahmed Fendri
Here we report the cDNA cloning of a phospholipase A2 (PLA2) from five Sparidae species. The deduced amino acid sequences show high similarity with pancreatic PLA2. In addition, a phylogenetic tree derived from alignment of various available sequences revealed that Sparidae PLA2 are closer to avian PLA2 group IB than to mammals' ones. In order to understand the structure-function relationships of these enzymes, we report here the recombinant expression in E.coli, the refolding and characterization of His-tagged annular seabream PLA2 (AsPLA2)...
June 14, 2017: Chemistry and Physics of Lipids
Jibin Song, Xiangyu Yang, Zhen Yang, Lisen Lin, Yijing Liu, Zijian Zhou, Zheyu Shen, Guocan Yu, Yunlu Dai, Orit Jacobson, Jeeva Munasinghe, Bryant Yung, Gao-Jun Teng, Xiaoyuan Chen
Reported procedures on the synthesis of gold nanoshells with smooth surfaces have merely demonstrated efficient control of shell thickness and particle size, yet no branch and nanoporous features on the nanoshell have been implemented to date. Herein, we demonstrate the ability to control the roughness and nanoscale porosity of gold nanoshells by using redox-active polymer poly(vinylphenol)-b-(styrene) nanoparticles as reducing agent and template. The porosity and size of the branches on this branched nanoporous gold nanoshell (BAuNSP) material can be facilely adjusted by control of the reaction speed or the reaction time between the redox-active polymer nanoparticles and gold ions (Au(3+))...
June 15, 2017: ACS Nano
James Townsend, Ruslan Burtovyy, Pavel Aprelev, Konstantin G Kornev, Igor Luzinov
This research is focused on the fabrication and properties of epoxy nanocomposites containing magnetized SiC whiskers (MSiCWs). To this end, we report an original strategy for fabrication of magnetically active SiCWs by decorating the whiskers with magnetic (iron oxide) nanoparticles via polymer-polymer (polyacrylic acid/poly(2-vinyl pyridine) complexation. The obtained whiskers demonstrated a substantial magnetic response in the polymerizing epoxy resin with application of only a 20 mT (200G) magnetic field...
June 9, 2017: ACS Applied Materials & Interfaces
Abir Ben Bacha, Ikram Jemel, Nadine M S Moubayed, Imen Ben Abdelmalek
Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was selected as a protease inhibitor source. The maximum recovery of the protease inhibitor against trypsin was recorded in the crude extract made in 0.1 M phosphate buffer (pH 7.0) and isolated from the mature leaves...
June 2017: 3 Biotech
Qiurui Zhang, Wenjuan Zhang, Natesan Mannangatti Rajendran, Tongling Liang, Wen-Hua Sun
A series of highly sensitive aluminum hydroquinolin-8-olates (C1-C8) was synthesized and characterized by (1)H/(13)C NMR spectroscopy. The molecular structures of compounds C1, C3, C4, and C5 were confirmed by single crystal X-ray crystallography and demonstrated the binuclear form. In the presence of BnOH, all the aluminum complexes exhibited moderate to high activities towards the ring-opening polymerization of ε-CL at high temperatures, but quite low activities at ambient temperature. Microstructure analysis of the resultant polycaprolactones showed that the polymers were linear in nature with a BnO- end group...
June 8, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
Priya Phadtare, Swati Joshi, T Satyanarayana
Codon adaptation index (CAI) of a 1263bp long endoglucanase encoding gene from the thermophilic mould Myceliopthora thermophile BJA has been improved from 0.44 to 0.76 by in vitro gene synthesis. The codon optimized endoglucanase gene (Mt-egl) has been constitutively expressed in Pichia pastoris under the regulation of GAP promoter. Recombinant endoglucanase (rMt-egl), purified by size exclusion chromatography, has been confirmed to be a monomeric protein of ∼47kDa. rMt-egl is optimally active at pH 10 and 50°C, displaying stability in broad pH and temperature ranges, with a t1/2 of 60 and 15min at 90 and 100°C, respectively...
June 4, 2017: International Journal of Biological Macromolecules
Luka Ausec, Francesca Berini, Carmine Casciello, Mariana Silvia Cretoiu, Jan Dirk van Elsas, Flavia Marinelli, Ines Mandic-Mulec
Metagenomics is a powerful tool that allows identifying enzymes with novel properties from the unculturable component of microbiomes. However, thus far only a limited number of laccase or laccase -like enzymes identified through metagenomics has been subsequently biochemically characterized. This work describes the successful bio-mining of bacterial laccase-like enzymes in an acidic bog soil metagenome and the characterization of the first acidobacterial laccase-like multicopper oxidase (LMCO). LMCOs have hitherto been mostly studied in fungi and some have already found applications in diverse industries...
June 6, 2017: Applied Microbiology and Biotechnology
Mukil Maruthamuthu, Jan Dirk van Elsas
BACKGROUND: Enzyme discovery is a promising approach to aid in the deconstruction of recalcitrant plant biomass in an industrial process. Novel enzymes can be readily discovered by applying metagenomics on whole microbiomes. Our goal was to select, examine, and characterize eight novel glycoside hydrolases that were previously detected in metagenomic libraries, to serve biotechnological applications with high performance. RESULTS: Here, eight glycosyl hydrolase family candidate genes were selected from metagenomes of wheat straw-degrading microbial consortia using molecular cloning and subsequent gene expression studies in Escherichia coli...
2017: Biotechnology for Biofuels
Jiang Li, Xiumeng Liu
A novel lipase gene lip256 was cloned and identified from the genomic library of hot spring strain Bacillus sp. HT19. The deduced amino acid sequence of lip256 has less than 32% identity to a predicted esterase (Cog1752) from Photobacterium leiognathi lrivu.4.1 and contains a novel motif (GTSAG) that differs from other clusters in the lipase superfamily. Following purification, a single band was obtained with a molecular mass of 33 kDa by SDS-PAGE, and the optimal temperature and pH for lipolytic activity of Lip25 were 70 °C and 9...
June 3, 2017: Lipids
Isadora Aumond Kuhn, Ari Roisenberg
The Rio dos Sinos Watershed area is located at the Middle-West region of the Rio Grande do Sul State, Southern Brazil, along thirty two municipalities and affecting 1.5 million inhabitants and many important industrial centers. Three main aquifers are recognized in the study area: the unconfined-fractured Serra Geral Aquifer System, the porous Guarani Aquifer System, and the Permian Aquitard. This study aims to understand groundwater, surface water and human activity interactions in the Rio dos Sinos Watershed, evaluating the application of stable lead isotopic ratios analyzed for this propose...
May 31, 2017: Journal of Environmental Radioactivity
M Schulze, C Ammon, J Schaefer, A-M Luther, M Jung, D Waberski
The dilution of ejaculates is a fundamental step for the production of liquid-preserved boar semen. For a long time, it has been recommended to add the extender to the ejaculate. The aim of the present study was to first compare the effect of the position ('center' vs. 'wall') where the extender is added to the semen-mixing cylinder (height 32.5cm; diameter 12.7cm) using an automatic dispenser (n=11). In experiment 2 (n=30), we analyzed the two main dilution methods (extender to the semen ('control') vs. 'reverse')...
May 29, 2017: Animal Reproduction Science
Maria Valeska Bianchi, Firas Awaja, George Altankov
Engineering dynamic stem cell niche-like environment offers opportunity to obtain better control of the fate of stem cells. We identified, for the first time, that periodic changes in the adhesive environment of human adipose derived mesenchymal stem cells (ADSCs) alters dramatically their asymmetric division but not their ability for symmetric renewal. Hereby, we used smart thermo-responsive polymer (PNIPAM) to create a dynamic adhesive environment for ADSCs by applying periodic temperature cycles to perturb adsorbed adhesive proteins to substratum interaction...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Weilong Shi, Feng Guo, Huibo Wang, Sijie Guo, Hao Li, Yunjie Zhou, Cheng Zhu, Yanhong Liu, Hui Huang, Baodong Mao, Yang Liu, Zhenhui Kang
Hydrogen production by photocatalytic overall water-splitting represents an ideal pathway for clean energy harvesting, for which developing high-efficiency catalysts has been the central scientific topic. Nanosized CoO with high solar-to-hydrogen efficiency (5%) is one of the most promising catalyst candidates. However, poor understanding of this photocatalyst leaves the key issue of rapid deactivation unclear and severely hinders its wide application. Here, we report a sub-micrometer CoO octahedron photocatalyst with high overall-water-splitting activity and outstanding ability of H2O2-resistance poisoning...
June 21, 2017: ACS Applied Materials & Interfaces
Qiu-Tong Li, Miao-Jie Jiang, Gang Wu, Li Chen, Si-Chong Chen, Yu-Xiao Cao, Yu-Zhong Wang
In the present work, we demonstrated the recyclability and precisely targeted reparability of amino functionalized multiwall carbon nanotubes-epoxy resin based on dynamic covalent Diels-Alder (DA) network (NH2-MWCNTs/DA-epoxy) by exploring the photothermal conversion of CNTs to trigger the reactions of dynamic chemical bonds. The covalent cross-linked networks of NH2-MWCNTs/DA-epoxy resin change their topology to linear polymer by thermally activated reverse Diels-Alder (r-DA) reactions at high temperatures, which endues the resin with almost 100% recyclability...
June 21, 2017: ACS Applied Materials & Interfaces
Shaofeng Lou, Xiuyuan Zhang, Mingming Zhang, Shenglu Ji, Weiwei Wang, Ju Zhang, Chen Li, Deling Kong
A reductase-cleavable and thermo-responsive star-shaped polymer nanogel was prepared via an "arm-first" atom transfer radical polymerization approach. The nanogel consists of a thermo- and redox-sensitive core and a zwitterionic copolymer block. The dual sensitive core is composed of poly(N-isopropylacrylamide) that is formed by disulfide crosslinking of N-isopropylacrylamide. The zwitterionic copolymer block contains a poly(sulfobetaine methacrylate) component, a known anti-adsorptive moiety that extends blood circulation time, and a lactose motif of poly(2-lactobionamidoethyl methacrylamide) that specifically targets the asialoglycoprotein receptors (ASGP-Rs) of hepatoma...
2017: International Journal of Nanomedicine
Puneet Gupta, Arjun K Mishra, Jyoti Vakhlu
A thermo-alkalistable and surfactant stable endoglucanase (PHS) gene consisting of 554 amino acids was identified from metagenomic library of Puga hot spring using functional screening. PHS gene was overexpressed and purified to homogeneity using affinity chromatography The purified PHS protein presented a single band of 60kDa on the SDS-PAGE gel and zymogram. The recombinant PHS exhibited activity over a broad range of pH and temperature with optima at pH 8.0 and 65°C, respectively and having optimum stability at 60°C and pH 8...
May 25, 2017: International Journal of Biological Macromolecules
Parameswaran Binod, Raveendran Sindhu, Aravind Madhavan, Amith Abraham, Anil Kuruvilla Mathew, Ummalyma Sabeela Beevi, Rajeev K Sukumaran, Sudhir P Singh, Ashok Pandey
l-glutaminases is an important industrial enzyme which finds potential applications in different sectors ranging from therapeutic to food industry. It is widely distributed in bacteria, actinomycetes, yeast and fungi. l-Glutaminases are mostly produced by Bacillus and Pseudomonas sp. and few reports were available with fungal, actinomycete and yeast system. Modern biotechnological tools help in the improved production as well as with tailor made properties for specific applications. Most of the genetic engineering studies were carried out for the production of l-glutaminase with improved thermo-tolerance and salt tolerance...
May 15, 2017: Bioresource Technology
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