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https://www.readbyqxmd.com/read/28332587/bioreactor-mechanically-guided-3d-mesenchymal-stem-cell-chondrogenesis-using-a-biocompatible-novel-thermo-reversible-methylcellulose-based-hydrogel
#1
A Cochis, S Grad, M J Stoddart, S Farè, L Altomare, B Azzimonti, M Alini, L Rimondini
Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro. Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. Moreover, MSC supporting material selection is fundamental to allow for an active interaction with cells. Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330236/xylanase-production-from-penicillium-citrinum-isolate-hzn13-using-response-surface-methodology-and-characterization-of-immobilized-xylanase-on-glutaraldehyde-activated-calcium-alginate-beads
#2
Zabin K Bagewadi, Sikandar I Mulla, Yogesh Shouche, Harichandra Z Ninnekar
The present study reports the production of high-level cellulase-free xylanase from Penicillium citrinum isolate HZN13. The variability in xylanase titers was assessed under both solid-state (SSF) and submerged (SmF) fermentation. SSF was initially optimized with different agro-waste residues, among them sweet sorghum bagasse was found to be the best substrate that favored maximum xylanase production (9643 U/g). Plackett-Burman and response surface methodology employing central composite design were used to optimize the process parameters for the production of xylanase under SSF...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328149/comparative-genomic-analyses-of-transport-proteins-encoded-within-the-red-algae-chondrus-crispus-galdieria-sulphuraria-and-cyanidioschyzon-merolae
#3
Justin Lee, Shounak Ghosh, Milton H Saier
Galdieria sulphuraria and Cyanidioschyzon merolae are thermo-acidophilic unicellular red algal cousins capable of living in volcanic environments, although the former can additionally thrive in the presence of toxic heavy metals. Bioinformatic analyses of transport systems were carried out on their genomes, as well as that of the mesophilic multicellular red alga Chondrus crispus (Irish moss). We identified transport proteins related to the metabolic capabilities, physiological properties, and environmental adaptations of these organisms...
March 22, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28294180/solar-mediated-thermo-electrochemical-oxidation-of-sodium-dodecyl-benzene-sulfonate-by-modulating-the-effective-oxidation-potential-and-pathway-for-green-remediation-of-wastewater
#4
Di Gu, Simeng Gao, TingTing Jiang, Baohui Wang
To match the relentless pursuit of three research hot points - efficient solar utilization, green and sustainable remediation of wastewater and advanced oxidation processes, solar-mediated thermo-electrochemical oxidation of surfactant was proposed and developed for green remediation of surfactant wastewater. The solar thermal electrochemical process (STEP), fully driven with solar energy to electric energy and heat and without an input of other energy, sustainably serves as efficient thermo-electrochemical oxidation of surfactant, exemplified by SDBS, in wastewater with the synergistic production of hydrogen...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28293826/utility-of-thermo-alkali-stable-%C3%AE-ca-from-polyextremophilic-bacterium-aeribacillus-pallidus-tshb1-in-biomimetic-sequestration-of-co2-and-as-a-virtual-peroxidase
#5
Himadri Bose, Tulasi Satyanarayana
Aeribacillus pallidus TSHB1 polyextremophilic bacterium produces a γ-carbonic anhydrase (ApCA), which is a homotrimeric biocatalyst with a subunit molecular mass of 32 ± 2 kDa. The enzyme is stable in the pH range between 8.0 and 11.0 and thus alkali-stable and moderately thermostable with T1/2 values of 40 ± 1, 15 ± 1, and 8 ± 0.5 min at 60, 70, and 80 °C, respectively. Activation energy for irreversible inactivation "E d " of carbonic anhydrase is 67.119 kJ mol(-1). The enzyme is stable in the presence of various flue gas contaminants such as SO3(2-),SO4(2-), and NO3(-) and cations Mg(2+), Mn(2+), Ca(2+), and Ba(2+)...
March 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28287123/near-field-terahertz-probes-with-room-temperature-nanodetectors-for-subwavelength-resolution-imaging
#6
Oleg Mitrofanov, Leonardo Viti, Enrico Dardanis, Maria Caterina Giordano, Daniele Ercolani, Antonio Politano, Lucia Sorba, Miriam S Vitiello
Near-field imaging with terahertz (THz) waves is emerging as a powerful technique for fundamental research in photonics and across physical and life sciences. Spatial resolution beyond the diffraction limit can be achieved by collecting THz waves from an object through a small aperture placed in the near-field. However, light transmission through a sub-wavelength size aperture is fundamentally limited by the wave nature of light. Here, we conceive a novel architecture that exploits inherently strong evanescent THz field arising within the aperture to mitigate the problem of vanishing transmission...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28282260/production-optimization-of-a-heat-tolerant-alkaline-pectinase-from-bacillus-subtilis-zgl14-and-its-purification-and-characterization
#7
Ping Yu, Yishu Zhang, Donglu Gu
Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize their concentrations. Starch, peptone, KH2PO4 and K2HPO4·3H2O were found to significantly affect the activity of alkaline pectinase. Their optimal concentrations were as follows: 4...
February 16, 2017: Bioengineered
https://www.readbyqxmd.com/read/28278112/purification-and-characterization-of-a-novel-trypsin-like-protease-from-green-seeded-chickpea-cicer-arientum
#8
Tooba Naz Shamsi, Romana Parveen, Priyankar Sen, Sadaf Fatima
The present study describes the purification and physicochemical and biochemical characterization of trypsin-like protease from green-seeded chickpea (Cicer arientum). The crude extract of chickpea trypsin (CpT) was obtained by homogenization followed by differential ammonium sulfate precipitation. The CpT was purified by ion-exchange chromatography on diethylaminoethyl (DEAE) column, pre-equilibrated with 20 mM tris-CaCl2 buffer (pH 8.2) with a flow rate of 0.5 mL min(-1). The molecular weight and purity of ∼23 kDa of CpT were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis...
February 10, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28254397/high-level-expression-of-two-thermophilic-%C3%AE-mannanases-in-yarrowialipolytica
#9
Wang YaPing, Rao Ben, Zhang Ling, Ma Lixin
Two thermophilic β-mannanases (ManA and ManB)were successfully expressed in Yarrowialipolytica using vector pINA1296I. The sequences of manA from Aspergillus niger CBS 513.88 and manB from Bacillus subtilis BCC41051 were optimized based on codon-usage bias in Y.lipolytica and synthesized by overlapping polymerase chain reaction (PCR). We utilized the pINA1296I vector, which allows inserting and expression of multiple copies of an expression cassette, to engineer recombinant strains containing multiple copies of manA or manB...
February 22, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28251389/construction-of-a-thermo-sensitive-pri857-vector-for-efficient-dna-capturing-in-escherichia-coli
#10
Kai Zhang, Huijuan Su, Muhan Yang, Jing Ge, Guiyao Li, Jun Yi, Yang Wang
OBJECTIVES: To establish a positive cloning system with a zero background for high-throughput DNA cloning purpose. RESULTS: The cloning vector, pRI857, and the genomic-library construction vector, pRI857-BAC, were constructed based on the mechanism of expression of the thermo-sensitive cI857 repressor gene that can stringently repress the PR promoter and kanamycin resistance gene (PR-kan (R) ) at 30 °C, but have no effect on PR-kan (R) gene at 37 °C or at higher temperatures...
March 1, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28241118/molecular-structure-of-lactoferrin-influences-the-thermal-resistance-of-lactococcal-phages
#11
Hany Geagea, Ahmed Gomaa, Gabriel Remondetto, Sylvain Moineau, Muriel Subirade
The protective effect of whey proteins on phages of lactic acid bacteria during heat treatment limits the recycling of whey proteins into cheese. To investigate this protective effect, we used lactoferrin (LF) as a whey protein model as a result of its unique physicochemical properties and its antiviral activity. First, the thermal inactivation of lactococcal thermoresistant virulent phage P1532 was measured in LF at 95 °C and under different pH values. Phage inactivation results revealed a strong protective effect of LF on P1532 phage at pH 5 but none at pH 7...
March 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28238734/drug-releasing-nanoplatforms-activated-by-alternating-magnetic-fields
#12
REVIEW
Damien Mertz, Olivier Sandre, Sylvie Bégin-Colin
The use of an alternating magnetic field (AMF) to generate non-invasively and spatially a localized heating from a magnetic nano-mediator has become very popular these last years to develop magnetic hyperthermia (MH) as a promising therapeutic modality already used in the clinics. AMF has become highly attractive this last decade over others radiations, as AMF allows a deeper penetration in the body and a less harmful ionizing effect. In addition to pure MH which induces tumor cell death through local T elevation, this AMF-generated magneto-thermal effect can also be exploited as a relevant external stimulus to trigger a drug release from drug-loaded magnetic nanocarriers, temporally and spatially...
February 24, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28236758/synergetic-effect-of-combined-pretreatment-for-energy-efficient-biogas-generation
#13
R Yukesh Kannah, S Kavitha, J Rajesh Banu, Ick Tae Yeom, M Johnson
Physiochemical disintegration of waste activated biosolids (WAB) through thermochemical (TC) pretreatment requires high energy and cost for efficient energy generation. Therefore in the present study, an attempt has been made to enhance the biodegrdability and to minimize the operational cost of TC pretreatment by combining it with ozonation. A higher solubilization of about 30.4% was achieved at lesser energy input of about 141.02kJ/kgTS and a ozone dosage of about 0.0012mgO3/mgSS through this combined thermo chemo ozone (TCO3) pretreatment...
February 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28204982/influence-of-carbon-sources-on-the-viability-and-resuscitation-of-acetobacter-senegalensis-during-high-temperature-gluconic-acid-fermentation
#14
Rasoul Shafiei, Raziyeh Zarmehrkhorshid, Majid Mounir, Philippe Thonart, Frank Delvigne
Much research has been conducted about different types of fermentation at high temperature, but only a few of them have studied cell viability changes during high-temperature fermentation. In this study, Acetobacter senegalensis, a thermo-tolerant strain, was used for gluconic acid production at 38 °C. The influences of different carbon sources and physicochemical conditions on cell viability and the resuscitation of viable but nonculturable (VBNC) cells formed during fermentation were studied. Based on the obtained results, A...
February 15, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28195065/effect-of-econazole-and-benzydamine-on-sensory-neurons-in-culture
#15
S Mathivanan, R de la Torre-Martinez, C Wolf, G Mangano, L Polenzani, C Milanese, A Ferrer-Montiel
Econazole is an anti-mycotic agent widely used for the treatment of cutaneous fungal infections, and for the therapy of vaginal candidiasis. Topical application of this azole is generally safe, although some patients have complained of mild burning sensation/cutaneous irritation and itching, especially when administered intravaginally. The underlying mechanisms responsible of these adverse effects are poorly understood, though they suggest excitation of cutaneous nociceptor terminals. We report that exposure of primary cultures of rat nociceptors to econazole augments neuronal excitability...
December 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28184988/cloning-evaluation-and-high-level-expression-of-a-thermo-alkaline-pectate-lyase-from-alkaliphilic-bacillus-clausii-with-potential-in-ramie-degumming
#16
Cheng Zhou, Yanfen Xue, Yanhe Ma
Alkaline pectate lyases (Pels) have potential application in bioscouring of the textile industry. In this study, a thermo-alkaline Pel (BacPelA) gene from an alkaliphilic Bacillus clausii strain was cloned and overexpressed in Escherichia coli. The mature BacPelA exhibited maximum activity at pH 10.5 and 70 °C and showed high cleavage capability on methylated pectins. BacPelA showed the highest specific activity of 936.2 U mg(-1) on ≥85% methylated pectin and 675.5 U mg(-1) on standard substrate polygalacturonic acid (PGA) upon evaluation of the absorbance at 235 nm (A235)...
February 9, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28183622/characterization-of-ginger-essential-oil-palygorskite-composite-geo-pgs-and-its-anti-bacteria-activity
#17
Hong Lei, Qiaonian Wei, Qing Wang, Anxiang Su, Mei Xue, Qin Liu, Qiuhui Hu
To explore a novel kind of anti-bacterial composite material having the excellent antibacterial ability, stability and specific-targeting capability, palygorskite (PGS) was used as the carrier of ginger essential oil (GEO) and a novel kind of composite GEO-PGS was prepared by ion exchange process. The characterization and the antibacterial activity of GEO-PGS was investigated in this study. Results of FTIR, XPS, XRD,TG analysis and SEM observation demonstrated the combination of GEO and PGS, GEO was absorbed on the surface of PGS, and the content of GEO in the composite was estimated to be 18...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28174088/casul-a-new-lectin-isolated-from-calliandra-surinamensis-leaf-pinnulae-with-cytotoxicity-to-cancer-cells-antimicrobial-activity-and-antibiofilm-effect
#18
Thamara Figueiredo Procópio, Leydianne Leite de Siqueira Patriota, Maiara Celine de Moura, Pollyanna Michelle da Silva, Ana Patrícia Silva de Oliveira, Lidiane Vasconcelos do Nascimento Carvalho, Thâmarah de Albuquerque Lima, Tatiana Soares, Túlio Diego da Silva, Luana Cassandra Breitenbach Barroso Coelho, Maira Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo, Regina Celia Bressan Queiroz de Figueiredo, Patrícia Maria Guedes Paiva, Thiago Henrique Napoleão
This work describes the isolation of a lectin (CasuL) from the leaf pinnulae of Calliandra surinamensis and the evaluation of its cytotoxic, antimicrobial and antibiofilm properties. Proteins from pinnulae extract were precipitated with ammonium sulphate (60% saturation) and submitted to Sephadex G-75 chromatography, which yielded isolated CasuL (purification factor: 113). Native CasuL is an acidic protein (pI 5.82) with a relative molecular mass of 48kDa. This lectin is also an oligomeric protein composed of three subunits and mass spectrometry revealed similarities with a Sorghum bicolor protein...
February 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28171839/optimization-of-operating-parameters-of-novel-composite-adsorbent-for-organic-pollutants-removal-from-pome-using-response-surface-methodology
#19
A O Adeleke, Ab Aziz Ab Latiff, A A Al-Gheethi, Zawawi Daud
The present work aimed to develop a novel composite material made up of activated cow bone powder (CBP) as a starting material for reducing chemical oxygen demand (COD) and ammonia-nitrogen (NH3N) from palm oil mill effluent (POME). The optimization of the reduction efficiency was investigated using response surface methodology (RSM). Six independent variables used in the optimization experiments include pH (4-10), speed (0.27-9.66 rcf), contact time (2-24 h), particle size (1-4.35 mm), dilution factor (100-500) and adsorbent dosage (65-125 g/L)...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/28170249/resolving-the-thermoinduced-electrochemistry-for-an-in-depth-understanding-of-the-step-degradation-of-sdbs
#20
Di Gu, Yiyang Zhang, Lingyue Zhu, Baohui Wang
The solar thermal electrochemical process (STEP) has sustainably accounted for the solar thermo- and electrochemical oxidation of sodium dodecyl benzene sulfonate (SDBS) fully driven by solar energy, gaining a high efficiency with a fast rate by the combination of thermochemistry and electrochemistry. In this article, thermoinduced electrochemistry was resolved for an in-depth understanding of the STEP degradation of SDBS. We employed thermodependent cyclic voltammetry, temperature-dependent fluorescence-electrochemical spectroscopy, and time-dependent electrochemical current spectroscopy for studying the electrochemistry, including the reaction, pathway, and mechanism...
February 16, 2017: Journal of Physical Chemistry. B
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