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https://www.readbyqxmd.com/read/28431941/bio-catalytic-performance-and-dye-based-industrial-pollutants-degradation-potential-of-agarose-immobilized-mnp-using-a-packed-bed-reactor-system
#1
Muhammad Bilal, Muhammad Asgher, Hafiz M N Iqbal, Hongbo Hu, Wei Wang, Xuehong Zhang
In this study, the matrix-entrapment technique was adopted to immobilize a novel manganese peroxidase (MnP). Agarose beads developed from 3.0% agarose concentration furnished the preeminent immobilization yield (92.76%). The immobilized MnP exhibited better resistance to changes in the pH and temperature as compared to the free counterpart, with optimal conditions being pH 6.0 and 45°C. Thermal and storage stability characteristics were significantly improved after immobilization, and the immobilized-MnP displayed higher tolerance against different temperatures than free MnP state...
April 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28415409/in-vitro-antiherpes-effect-of-c-glycosyl-flavonoid-enriched-fraction-of-cecropia-glaziovii-encapsulated-in-plga-nanoparticles
#2
Talitha Caldas Dos Santos, Nicoletta Rescignano, Laurita Boff, Flávio Henrique Reginatto, Cláudia Maria Oliveira Simões, Angela Machado de Campos, Carmen Mijangos
In this work is reported a novel and promising approach for the preparation of C-glycosylflavonoid enriched fraction of Cecropia glaziovii (EFF-Cg) loaded PLGA nanoparticles (NP) with antiherpes properties. The purpose of this study was to evaluate and to compare the effect of two nonionic surfactants (poloxamer 188 (PLU) and polyvinyl alcohol (PVA)), and also an emulsion stabilized by solid particles of cellulose nanocrystal (CNC) in place of surfactants. The characterization of these nanoparticles was in terms of size, polydispersity index, zeta potential, morphology, thermogravimetric analysis (TGA), loading capacity and percent yield...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28412143/thermo-sensitive-assembly-of-the-biomaterial-rep-reduces-hematoma-volume-following-collagenase-induced-intracerebral-hemorrhage-in-rats
#3
Joohyun Park, Jong Youl Kim, Seong-Kyoon Choi, Jae Young Kim, Jae Hwan Kim, Won Bae Jeon, Jong Eun Lee
Intracerebral hemorrhage (ICH) frequently results in severe disabilities and high mortality. RGD-containing Elastin-Like Polypeptide (REP), a modified Elastin-Like Polypeptide (ELP), is a thermally responsive biopolymer. REP has high affinity for cells and is known to show non-immunotoxicity, -cytotoxicity, and -inflammatory responses. Here we found that administration of REP in the acute phase of the ICH rat model reduced the hematoma volume, decreased the number of activated microglia, attenuated the expression of von Willebrand Factor (vWF), and prevented the leakage of Immunoglobulin G (IgG) into the cerebral parenchyma without any occlusion of intact microvessels...
April 12, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28411569/melamine-formaldehyde-derived-porous-carbons-for-adsorption-of-co2-capture
#4
Deepak Tiwari, Chitrakshi Goel, Haripada Bhunia, Pramod K Bajpai
In this work, we report carbon adsorbents obtained from high nitrogen content melamine-formaldehyde resin as starting material and mesoporous zeolite MCM-41 as template through nanocasting technique. To synthesize different carbon structure adsorbents with improved textural and surface properties, the material undergo carbonization followed by physical activation under CO2 atmosphere at different temperatures. Characterizations of the adsorbents using SEM, TEM, XPS, nitrogen sorption, CHN, TKN, and TPD have been carried out...
April 12, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28407915/distribution-of-apparent-activation-energy-counterparts-during-thermo-and-thermo-oxidative-degradation-of-aronia-melanocarpa-black-chokeberry
#5
Bojan Janković, Milena Marinović-Cincović, Marija Janković
Kinetics of degradation for Aronia melanocarpa fresh fruits in argon and air atmospheres were investigated. The investigation was based on probability distributions of apparent activation energy of counterparts (εa). Isoconversional analysis results indicated that the degradation process in an inert atmosphere was governed by decomposition reactions of esterified compounds. Also, based on same kinetics approach, it was assumed that in an air atmosphere, the primary compound in degradation pathways could be anthocyanins, which undergo rapid chemical reactions...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28397813/nonlinear-dynamics-and-chaos-in-an-optomechanical-beam
#6
Daniel Navarro-Urrios, Néstor E Capuj, Martín F Colombano, P David García, Marianna Sledzinska, Francesc Alzina, Amadeu Griol, Alejandro Martínez, Clivia M Sotomayor-Torres
Optical nonlinearities, such as thermo-optic mechanisms and free-carrier dispersion, are often considered unwelcome effects in silicon-based resonators and, more specifically, optomechanical cavities, since they affect, for instance, the relative detuning between an optical resonance and the excitation laser. Here, we exploit these nonlinearities and their intercoupling with the mechanical degrees of freedom of a silicon optomechanical nanobeam to unveil a rich set of fundamentally different complex dynamics...
April 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/28396838/thermo-kinetic-analysis-space-expansion-for-cyclophilin-ligand-interactions-identification-of-a-new-nonpeptide-inhibitor-using-biacore%C3%A2-t200
#7
Martin A Wear, Matthew W Nowicki, Elizabeth A Blackburn, Iain W McNae, Malcolm D Walkinshaw
We have established a refined methodology for generating surface plasmon resonance sensor surfaces of recombinant his-tagged human cyclophilin-A. Our orientation-specific stabilisation approach captures his-tagged protein under 'physiological conditions' (150 mm NaCl, pH 7.5) and covalently stabilises it on Ni(2+)-nitrilotriacetic acid surfaces, very briefly activated for primary amine-coupling reactions, producing very stable and active surfaces (≥ 95% specific activity) of cyclophilin-A. Variation in protein concentration with the same contact time allows straightforward generation of variable density surfaces, with essentially no loss of activity, making the protocol easily adaptable for studying numerous interactions; from very small fragments, ~ 100 Da, to large protein ligands...
April 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28394282/thermo-oxidative-stability-evaluation-of-bullfrog-rana-catesbeiana-shaw-oil
#8
Renata Rutckeviski, Francisco H Xavier-Júnior, Andreza R V Morais, Éverton N Alencar, Lucas Amaral-Machado, Julieta Genre, Amanda D Gondim, Eryvaldo S T Egito
Bullfrog oil (BO), a natural product obtained from recycling of adipose tissue from the amphibian Rana catesbeiana Shaw, has been recently evaluated as a therapeutic activity ingredient. This work aimed to evaluate the long-term and accelerated thermal oxidative stabilities of this product, which is a promising raw material for emulsion technology development. BO was extracted from amphibian adipose tissue at 70 °C with a yield of 60% ± 0.9%. Its main fatty acid compounds were oleic (30.0%) and eicosapentaenoic (17...
April 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28384593/thermo-acid-stable-phytase-mediated-enhancement-of-bioethanol-production-using-colocasia-esculenta
#9
Melvin Makolomakwa, Adarsh Kumar Puri, Kugen Permaul, Suren Singh
Phytase production by the thermophilic mould Thermomyces lanuginosus SSBP was enhanced 8.56-fold in submerged fermentation, which was further improved in fed-batch cultivations. The protein was purified to homogeneity using ammonium sulphate precipitation, Resource Q anion exchange and Superdex gel-filtration chromatography, with an overall purification of 24.7-fold and a yield of 5.16%. The purified 49kDa protein was optimally active at 55°C and pH 5.0, and was stable between 50 and 90°C from pH 3.0-6.0, with a half-life of 138...
March 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28381063/method-to-simulate-and-analyse-induced-stresses-for-laser-crystal-packaging-technologies
#10
Pol Ribes-Pleguezuelo, Site Zhang, Erik Beckert, Ramona Eberhardt, Frank Wyrowski, Andreas Tünnermann
A method to simulate induced stresses for a laser crystal packaging technique and the consequent study of birefringent effects inside the laser cavities has been developed. The method has been implemented by thermo-mechanical simulations implemented with ANSYS 17.0. ANSYS results were later imported in VirtualLab Fusion software where input/output beams in terms of wavelengths and polarization were analysed. The study has been built in the context of a low-stress soldering technique implemented for glass or crystal optics packaging's called the solderjet bumping technique...
March 20, 2017: Optics Express
https://www.readbyqxmd.com/read/28380985/active-phase-correction-of-high-resolution-silicon-photonic-arrayed-waveguide-gratings
#11
M Gehl, D Trotter, A Starbuck, A Pomerene, A L Lentine, C DeRose
Arrayed waveguide gratings provide flexible spectral filtering functionality for integrated photonic applications. Achieving narrow channel spacing requires long optical path lengths which can greatly increase the footprint of devices. High index contrast waveguides, such as those fabricated in silicon-on-insulator wafers, allow tight waveguide bends which can be used to create much more compact designs. Both the long optical path lengths and the high index contrast contribute to significant optical phase error as light propagates through the device...
March 20, 2017: Optics Express
https://www.readbyqxmd.com/read/28358283/characterization-of-a-thermo-alkali-stable-laccase-from-bacillus-subtilis-cjp3-and-its-application-in-dyes-decolorization
#12
Weichuan Qiao, Jingping Chu, Shaojun Ding, Xin Song, Lei Yu
In this work, a novel bacterial strain exhibiting laccase activity was isolated from black liquor and identified as Bacillus subtilis cjp3. The CotA-laccase gene was cloned from strain cjp3 and expressed in Escherichia coli. The purified recombinant laccase has a maximum activity of 7320 U/L, maintaining high stabilities under a wide pH range and high temperature conditions. Nearly no loss of laccase activity was observed even at pH 9.0 after 10 h of incubation. Reactive blue 19, reactive black 5 and indigo carmine could be efficiently decolorized by the purified laccase in the presence of a mediator ABTS...
March 30, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28349153/al-doped-cop-nanoarray-a-durable-water-splitting-electrocatalyst-with-superhigh-activity
#13
Rong Zhang, Chun Tang, Rongmei Kong, Gu Du, Abdullah M Asiri, Liang Chen, Xuping Sun
The scalable production of hydrogen fuel through electrochemical water reduction needs efficient Earth-abundant electrocatalysts to make the whole water-splitting process more energy efficient. In this Article, we report that an Al-doped CoP nanoarray on carbon cloth (Al-CoP/CC) behaves as a durable hydrogen evolution electrocatalyst with superhigh activity in 0.5 M H2SO4. It demands a pretty low overpotential of 23 mV to drive a geometrical catalytic current density of 10 mA cm(-2), outperforming all reported non-precious metal catalysts...
March 28, 2017: Nanoscale
https://www.readbyqxmd.com/read/28344094/controlled-release-of-antibiotic-amoxicillin-drug-using-carboxymethyl-cellulose-cl-poly-lactic-acid-co-itaconic-acid-hydrogel
#14
S Sood, Vinod Kumar Gupta, Shilpi Agarwal, Kamal Dev, Deepak Pathania
In this paper, microwave assisted preparation of carboxymethyl cellulose-cl-poly(lactic acid-co-itaconic acid) (CMC-cl-P(LA-co-IA)) hydrogel was reported via facile graft copolymerization using N,N(1)-methylene-bis-acrylamide (MBA) and potassium persulphate as cross linker and initiator. Different reaction parameters were optimized to achieve good yield. The formation of hydrogel was confirmed by characterization techniques such as Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction, thermo gravimetric analysis, transmission electron microscopy etc...
March 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28332587/bioreactor-mechanically-guided-3d-mesenchymal-stem-cell-chondrogenesis-using-a-biocompatible-novel-thermo-reversible-methylcellulose-based-hydrogel
#15
A Cochis, S Grad, M J Stoddart, S Farè, L Altomare, B Azzimonti, M Alini, L Rimondini
Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro. Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. Moreover, MSC supporting material selection is fundamental to allow for an active interaction with cells. Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330236/xylanase-production-from-penicillium-citrinum-isolate-hzn13-using-response-surface-methodology-and-characterization-of-immobilized-xylanase-on-glutaraldehyde-activated-calcium-alginate-beads
#16
Zabin K Bagewadi, Sikandar I Mulla, Yogesh Shouche, Harichandra Z Ninnekar
The present study reports the production of high-level cellulase-free xylanase from Penicillium citrinum isolate HZN13. The variability in xylanase titers was assessed under both solid-state (SSF) and submerged (SmF) fermentation. SSF was initially optimized with different agro-waste residues, among them sweet sorghum bagasse was found to be the best substrate that favored maximum xylanase production (9643 U/g). Plackett-Burman and response surface methodology employing central composite design were used to optimize the process parameters for the production of xylanase under SSF...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328149/comparative-genomic-analyses-of-transport-proteins-encoded-within-the-red-algae-chondrus-crispus-galdieria-sulphuraria-and-cyanidioschyzon-merolae
#17
Justin Lee, Shounak Ghosh, Milton H Saier
Galdieria sulphuraria and Cyanidioschyzon merolae are thermo-acidophilic unicellular red algal cousins capable of living in volcanic environments, although the former can additionally thrive in the presence of toxic heavy metals. Bioinformatic analyses of transport systems were carried out on their genomes, as well as that of the mesophilic multicellular red alga Chondrus crispus (Irish moss). We identified transport proteins related to the metabolic capabilities, physiological properties, and environmental adaptations of these organisms...
March 22, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28294180/solar-mediated-thermo-electrochemical-oxidation-of-sodium-dodecyl-benzene-sulfonate-by-modulating-the-effective-oxidation-potential-and-pathway-for-green-remediation-of-wastewater
#18
Di Gu, Simeng Gao, TingTing Jiang, Baohui Wang
To match the relentless pursuit of three research hot points - efficient solar utilization, green and sustainable remediation of wastewater and advanced oxidation processes, solar-mediated thermo-electrochemical oxidation of surfactant was proposed and developed for green remediation of surfactant wastewater. The solar thermal electrochemical process (STEP), fully driven with solar energy to electric energy and heat and without an input of other energy, sustainably serves as efficient thermo-electrochemical oxidation of surfactant, exemplified by SDBS, in wastewater with the synergistic production of hydrogen...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28293826/utility-of-thermo-alkali-stable-%C3%AE-ca-from-polyextremophilic-bacterium-aeribacillus-pallidus-tshb1-in-biomimetic-sequestration-of-co2-and-as-a-virtual-peroxidase
#19
Himadri Bose, Tulasi Satyanarayana
Aeribacillus pallidus TSHB1 polyextremophilic bacterium produces a γ-carbonic anhydrase (ApCA), which is a homotrimeric biocatalyst with a subunit molecular mass of 32 ± 2 kDa. The enzyme is stable in the pH range between 8.0 and 11.0 and thus alkali-stable and moderately thermostable with T1/2 values of 40 ± 1, 15 ± 1, and 8 ± 0.5 min at 60, 70, and 80 °C, respectively. Activation energy for irreversible inactivation "E d " of carbonic anhydrase is 67.119 kJ mol(-1). The enzyme is stable in the presence of various flue gas contaminants such as SO3(2-),SO4(2-), and NO3(-) and cations Mg(2+), Mn(2+), Ca(2+), and Ba(2+)...
March 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28287123/near-field-terahertz-probes-with-room-temperature-nanodetectors-for-subwavelength-resolution-imaging
#20
Oleg Mitrofanov, Leonardo Viti, Enrico Dardanis, Maria Caterina Giordano, Daniele Ercolani, Antonio Politano, Lucia Sorba, Miriam S Vitiello
Near-field imaging with terahertz (THz) waves is emerging as a powerful technique for fundamental research in photonics and across physical and life sciences. Spatial resolution beyond the diffraction limit can be achieved by collecting THz waves from an object through a small aperture placed in the near-field. However, light transmission through a sub-wavelength size aperture is fundamentally limited by the wave nature of light. Here, we conceive a novel architecture that exploits inherently strong evanescent THz field arising within the aperture to mitigate the problem of vanishing transmission...
March 13, 2017: Scientific Reports
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