Philippe de Diesbach, Thierry Medts, Sarah Carpentier, Ludovic D'Auria, Patrick Van Der Smissen, Anna Platek, Marcel Mettlen, Adrian Caplanusi, Marie-France van den Hove, Donatienne Tyteca, Pierre J Courtoy
Most Src family members are diacylated and constitutively associate with membrane "lipid rafts" that coordinate signalling. Whether the monoacylated Src, frequently hyperactive in carcinomas, also localizes at "rafts" remains controversial. Using polarized MDCK cells expressing the thermosensitive v-Src/tsLA31 variant, we here addressed how Src tyrosine-kinase activation may impact on its (i) membrane recruitment, in particular to "lipid rafts"; (ii) subcellular localization; and (iii) signalling. The kinetics of Src-kinase thermoactivation correlated with its recruitment from the cytosol to sedimentable membranes where Src largely resisted solubilisation by non-ionic detergents at 4 degrees C and floated into sucrose density gradients like caveolin-1 and flotillin-2, i...
April 15, 2008: Experimental Cell Research