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https://www.readbyqxmd.com/read/29216572/gene-expression-profiling-of-astaxanthin-and-fatty-acid-pathways-in-haematococcus-pluvialis-in-response-to-different-led-lighting-conditions
#1
Ruijuan Ma, Skye R Thomas-Hall, Elvis T Chua, Faisal Alsenani, Eladl Eltanahy, Michael E Netzel, Gabriele Netzel, Yinghua Lu, Peer M Schenk
Haematococcus pluvialis is a green microalga of major interest to industry based on its ability to produce large amounts of astaxanthin. Biosynthesis of astaxanthin and its mono- and di-esters was significantly stimulated under 150 μmol m-2 s-1 of white LED (W-150) compared with lower light intensities, but the highest astaxanthin amounts were produced under 70 μmol m-2 s-1 of blue LED (B-70). Transcripts of astaxanthin biosynthesis genes psy, crtO, and bkt2 were upregulated under W-150, while psy, lcy, crtO, and crtR-B were upregulated by B-70...
December 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29201791/psychological-results-of-438-patients-with-persisting-gastroesophageal-reflux-disease-symptoms-by-symptom-checklist-90-revised-questionnaire
#2
Xia Chen, Ping Li, Fei Wang, Guozhong Ji, Lin Miao, Sihong You
Aims and objectives: Gastroesophageal reflux disease (GERD) affects mental state and social activities. On the contrary, mental disorders may also play a crucial role in GERD symptoms. The purpose of the study was to analyze the data of Symptom Checklist 90-Revised (SCL-90-R) questionnaire from patients with persisting GERD and to explore the impact of psychological factors on them. Materials and methods: The patients accepted SCL-90-R questionnaire survey, following endoscopy, high-resolution manometry (HRM), and ambulatory impedance-pH monitoring...
July 2017: Euroasian Journal of Hepato-Gastroenterology
https://www.readbyqxmd.com/read/29182721/molecular-mimicry-modulates-plant-host-responses-to-pathogens
#3
Pamela Ronald, Anna Joe
Background: Pathogens often secrete molecules that mimic those present in the plant host. Recent studies indicate that some of these molecules mimic plant hormones required for development and immunity. Scope and Conclusion: This Viewpoint reviews the literature on microbial molecules produced by plant pathogens that functionally mimic molecules present in the plant host. This article includes examples from nematodes, bacteria and fungi with emphasis on RaxX, a microbial protein produced by the bacterial pathogen Xanthomonas oryzae pv...
November 22, 2017: Annals of Botany
https://www.readbyqxmd.com/read/29172590/the-mediating-effect-of-family-cohesion-in-reducing-patient-symptoms-and-family-distress-in-a-culturally-informed-family-therapy-for-schizophrenia-a-parallel-process-latent-growth-model
#4
Caitlin A Brown, Amy Weisman de Mamani
OBJECTIVE: Although both patients with schizophrenia and their caregivers report elevated levels of depression, anxiety, and stress (DASS), affective symptoms in patients and family members seldom constitute a primary treatment focus. The present study tested whether a culturally informed family therapy for schizophrenia (CIT-S) outperformed standard family psychoeducation (PSY-ED) not only in decreasing patient schizophrenia symptoms, but also in decreasing individual DASS. Because CIT-S fostered family cohesion throughout treatment, we predicted that increases in family cohesion would mediate treatment effects...
November 27, 2017: Journal of Consulting and Clinical Psychology
https://www.readbyqxmd.com/read/29155321/clp-protease-and-or-directly-control-the-proteostasis-of-phytoene-synthase-the-crucial-enzyme-for-carotenoid-biosynthesis-in-arabidopsis
#5
Ralf Welsch, Xiangjun Zhou, Hui Yuan, Daniel Álvarez, Tianhu Sun, Dennis Schlossarek, Yong Yang, Guoxin Shen, Hong Zhang, Manuel Rodriguez-Concepcion, Theodore W Thannhauser, Li Li
Phytoene synthase (PSY) is the crucial plastidial enzyme in the carotenoid biosynthetic pathway. However, its post-translational regulation remains elusive. Likewise, Clp protease constitutes a central part of the plastid protease networks, but its substrates for degradation are not well known. In this study, we report that PSY is a substrate of the Clp protease. PSY was uncovered to physically interact with various Clp protease subunits (i.e. ClpS1, ClpC1, and ClpD). High levels of PSY and several other carotenogenic enzymes over-accumulate in the clpc1, clpp4 and clpr1-2 mutants...
November 16, 2017: Molecular Plant
https://www.readbyqxmd.com/read/29111068/microwave-assisted-synthesis-of-binary-grafted-psyllium-and-its-utility-in-anticancer-formulation
#6
Deepak Kumar, Jyoti Pandey, Pramendra Kumar
A binary grafted copolymer of Psyllium mucilage (Psy) with acrylic acid (AA) and acrylonitrile (An) has been successfully synthesized under microwave conditions for in vitro drug release study. The grafting was confirmed by FTIR spectroscopy, XRD, SEM, EDX, TGA analytical techniques and the intrinsic viscosity study. The swelling behavior of grafted material has been studied in solution of different pH and time. We also prepare Psy-g-Poly (AA-co-An) based beads with anti-cancer drug [(2-Chloro-3-(4-hydroxyphenylamino) naphthalene-1, 4-dione)]...
January 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29109314/a-cross-sectional-study-to-assess-the-perception-of-psychosocial-elements-among-pediatric-patients-visiting-dental-clinics
#7
Ashish Bhalla, U B Rajasekaran, Madhavi Singh, Manish Goutam, Nikhil Grover, Ashok Galav
INTRODUCTION: Children with high dental fear and perception usually encounter high prevalence of dental caries. Data showed that individuals with dental fear will probably defer dental appointments which may result in the formation of a setup of an endless loop of dental fear. Present study was aimed to assess different psycho-social parameters among pediatric patients visiting dental clinics. MATERIALS AND METHODS: In the present cross-sectional study, the data of 200 schoolchildren were collected and analyzed by means of a questionnaire...
November 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/29094166/the-relationship-between-high-order-aberration-and-anterior-ocular-biometry-during-accommodation-in-young-healthy-adults
#8
Bilian Ke, Xinjie Mao, Hong Jiang, Jichang He, Che Liu, Min Li, Ying Yuan, Jianhua Wang
Purpose: This study investigated the anterior ocular anatomic origin of high-order aberration (HOA) components using optical coherence tomography and a Shack-Hartmann wavefront sensor. Methods: A customized system was built to simultaneously capture images of ocular wavefront aberrations and anterior ocular biometry. Relaxed, 2-diopter (D) and 4-D accommodative states were repeatedly measured in 30 young subjects. Custom software was used to correct optical distortions and measure biometric parameters from the images...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29091762/tfcp2-is-required-for-yap-dependent-transcription-to-stimulate-liver-malignancy
#9
Xiao Zhang, Fenyong Sun, Yongxia Qiao, Weisheng Zheng, Ya Liu, Yan Chen, Qi Wu, Xiangfan Liu, Guoqing Zhu, Yuxin Chen, Yongchun Yu, Qiuhui Pan, Jiayi Wang
Although YAP-dependent transcription is closely associated with liver tumorigenesis, the mechanism by which YAP maintains its function is poorly understood. Here, we show that TFCP2 is required for YAP-dependent transcription and liver malignancy. Mechanistically, YAP function is stimulated by TFCP2 via a WW-PSY interaction. TFCP2 also maintains YAP stability by inhibiting βTrCP. Notably, genomic co-occupancy of YAP and TFCP2 is revealed. TFCP2 acts as a transcription co-factor that stimulates YAP transcription by facilitating YAP binding with YAP binding motif (YBF)-containing transcription factors...
October 31, 2017: Cell Reports
https://www.readbyqxmd.com/read/29076614/the-predictive-role-of-health-promoting-behaviors-and-perceived-stress-in-aneurysmal-rupture
#10
Mi-Sun Lee, Chang G Park, Tonda L Hughes, Sang-Eun Jun, Kum Whang, Nahyun Kim
AIM AND OBJECTIVE: We examined the roles of two modifiable factors-health-promoting behaviors (HPB) and perceived stress-in predicting aneurysmal rupture. BACKGROUND: Unruptured intracranial aneurysm (UIA) detection produces significant stress and anxiety in patients because of the risk of rupture. Compared to non-modifiable risk factors for rupture such as age, gender, and aneurysm size/location, less attention has been given to modifiable risk factors. Two modifiable factors, HPBs and perceived stress, have hardly been examined as potential predictors of rupture...
October 27, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28992607/effect-of-uv-radiation-and-its-implications-on-carotenoid-pathway-in-bixa-orellana-l
#11
M Sankari, H Hridya, P Sneha, C George Priya Doss, Siva Ramamoorthy
The current study was undertaken to analyse the effect of short-term UV-B and UV-C radiations in provoking carotenoid biosynthesis in Bixa orellana. Seeds of B. orellana were germinated and exposed to the short term UV pre-treatment under controlled environmental condition for 5days. The UV treated young seedlings response in pigment contents; antioxidant enzyme activity and mRNA gene expression level were analysed. The pigment content such as chlorophyll was increased in both UV-B and UV-C treated seedlings, but the total carotenoid level was decreased when compared to the control seedlings this can be attributed to the plant adaptability to survive in a stressed condition...
October 2, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28985609/inhibition-of-autophagy-modulates-astaxanthin-and-total-fatty-acid-biosynthesis-in-chlorella-zofingiensis-under-nitrogen-starvation
#12
Zhao Zhang, Dongzhe Sun, Ka-Wing Cheng, Feng Chen
The present study showed that inhibition of autophagy significantly increased cellular levels of reactive oxygen species in Chlorella zofingiensis under nitrogen starvation. This was accompanied with increased expression of PSY, and enhanced accumulation of astaxanthin after 48h of cultivation. Nevertheless, the proportion of astaxanthin in secondary carotenoids remained unchanged. Meanwhile, the expression level of ACCase was also elevated in the 3-MA-treated cells compared to the control despite a >20% lower content of fatty acid in the former than the latter...
September 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28936220/de-novo-assembly-of-the-camellia-nitidissima-transcriptome-reveals-key-genes-of-flower-pigment-biosynthesis
#13
Xingwen Zhou, Jiyuan Li, Yulin Zhu, Sui Ni, Jinling Chen, Xiaojuan Feng, Yunfeng Zhang, Shuangquan Li, Hongguang Zhu, Yuanguang Wen
The golden camellia, Camellia nitidissima Chi., is a well-known ornamental plant that is known as "the queen of camellias" because of its golden yellow flowers. The principal pigments in the flowers are carotenoids and flavonol glycosides. Understanding the biosynthesis of the golden color and its regulation is important in camellia breeding. To obtain a comprehensive understanding of flower development in C. nitidissima, a number of cDNA libraries were independently constructed during flower development. Using the Illumina Hiseq2500 platform, approximately 71...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28927510/the-development-and-validation-of-the-patient-participation-culture-tool-for-inpatient-psychiatric-wards-pact-psy
#14
Simon Malfait, Joeri Vandewalle, Kristof Eeckloo, Roos Colman, Ann Van Hecke
Patient participation is an important topic in mental health and receives increased attention along with deinstitutionalization. No tool exists to measure healthcare worker-related factors that influence patient participation. A three-staged study was conducted to develop and validate the 'Patient Participation Culture Tool for inpatient PSYchiatric wards' (PaCT-PSY), and to analyse its psychometric properties (n=603). The 60-items tool, comprising thirteen components, showed content validity, strong psychometric properties, and a high internal consistency...
October 2017: Archives of Psychiatric Nursing
https://www.readbyqxmd.com/read/28885552/structure-of-pigment-metabolic-pathways-and-their-contributions-to-white-tepal-color-formation-of-chinese-narcissus-tazetta-var-chinensis-cv-jinzhanyintai
#15
Yujun Ren, Jingwen Yang, Bingguo Lu, Yaping Jiang, Haiyang Chen, Yuwei Hong, Binghua Wu, Ying Miao
Chinese narcissus (Narcissus tazetta var. chinensis) is one of the ten traditional flowers in China and a famous bulb flower in the world flower market. However, only white color tepals are formed in mature flowers of the cultivated varieties, which constrains their applicable occasions. Unfortunately, for lack of genome information of narcissus species, the explanation of tepal color formation of Chinese narcissus is still not clear. Concerning no genome information, the application of transcriptome profile to dissect biological phenomena in plants was reported to be effective...
September 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28871021/effectiveness-of-two-psychological-interventions-for-pain-management-emotional-regulation-and-promotion-of-quality-of-life-among-adult-portuguese-men-with-haemophilia-psy-haemopeq-study-protocol-for-a-single-centre-prospective-randomised-controlled-trial
#16
Patrícia Ribeiro Pinto, Ana Cristina Paredes, Patrício Costa, Manuela Carvalho, Manuela Lopes, Susana Fernandes, Susana Pedras, Armando Almeida
INTRODUCTION: Haemophilia is a bleeding disorder associated with significant pain, emotional distress, quality of life (QoL) impairment and considerable healthcare costs. Psychosocial health and effective pain management are considered essential end points for optimal haemophilia care, but there is a significant gap in evidence-based treatments targeting these outcomes in people with haemophilia (PWH). Psychological interventions are cost-effective in promoting emotional well-being, QoL and pain control, although these have been scarcely used in haemophilia field...
September 3, 2017: BMJ Open
https://www.readbyqxmd.com/read/28865139/expression-of-synthetic-phytoene-synthase-gene-to-enhance-%C3%AE-carotene-production-in-scenedesmus-sp-cpc2
#17
Chun-Yen Chen, Ai-Ling Kao, Zheng-Chia Tsai, Yu-Mei Shen, Pei-Hsun Kao, I-Son Ng, Jo-Shu Chang
β-carotene is a valuable pigment abundant in some microalgal species but the low β-carotene productivity of microalgae has become the major obstacles against its commercialization. This work aims to improve the productivity of algae-based β-carotene via genetic engineering approaches. First, a synthetic psy gene construct (891 bp) encoding 297 amino acids is expressed in Scenedesmus sp. CPC2 host to enhance the β-carotene production. The synthetic psy gene is designed by considering the highest consensus of amino acids (i...
September 2, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28858245/molecular-cloning-and-characterization-of-carotenoid-pathway-genes-and-carotenoid-content-in-ixeris-dentata-var-albiflora
#18
Chinreddy Subramanyam Reddy, Sang-Hoon Lee, Jeong Su Yoon, Jae Kwang Kim, Sang Won Lee, Mok Hur, Sung Cheol Koo, Jin Meilan, Woo Moon Lee, Jae Ki Jang, Yoonkang Hur, Sang Un Park, And Yeon Bok Kim
Ixeris dentata var. albiflora is considered as a potential therapeutic agent against mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors as well as good seasoned vegetable in Far East countries. Phytoene synthase (PSY), phytoene desaturase (PDS) ξ-carotene desaturase (ZDS), lycopene β-cyclase (LCYB), lycopene ε-cyclase (LCYE), ε-ring carotene hydroxylase (CHXB), and zeaxanthin epoxidase (ZDS) are vital enzymes in the carotenoid biosynthesis pathway. We have examined these seven genes from I...
August 31, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28846584/effects-of-preoperative-psychological-interventions-on-catecholamine-and-cortisol-levels-after-surgery-in-coronary-artery-bypass-graft-patients-the-randomized-controlled-psy-heart-trial
#19
Stefan Salzmann, Frank Euteneuer, Johannes A C Laferton, Charlotte J Auer, Meike C Shedden-Mora, Manfred Schedlowski, Rainer Moosdorf, Winfried Rief
OBJECTIVE: The aim of the study was to examine whether preoperative psychological interventions targeting patients' expectations are capable of influencing the biological stress response after coronary artery bypass graft (CABG) surgery and could thus improve recovery after heart surgery. METHODS: Randomized controlled trial with assessments 10 days before surgery, after psychological intervention (day of hospital admission, but before surgery), postoperative (6-8 days later), and at follow-up (6 months after surgery)...
September 2017: Psychosomatic Medicine
https://www.readbyqxmd.com/read/28818371/assessment-of-molecular-and-epigenetic-changes-in-the-albinism-of-agave-angustifolia-haw
#20
Rosa Us-Camas, Eduardo Castillo-Castro, Margarita Aguilar-Espinosa, Verónica Limones-Briones, Renata Rivera-Madrid, Manuel L Robert-Díaz, Clelia De-la-Peña
Albinism in plants is a rare phenomenon that occurs in nature and is characterized by the total or partial loss of photosynthetic pigments. Although progress has been made in understanding the nature of this phenomenon, the precise causes and biological basis are still unexplored. Here, we study the genetic and epigenetic differences between green (G), variegated (V) and albino (A) A. angustifolia Haw. plantlets obtained by in vitro propagation in order to present new insights into albinism from a plant system that offers a unique set of biological phenotypic characteristics...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
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