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https://www.readbyqxmd.com/read/29463773/the-citrus-transcription-factor-csmads6-modulates-carotenoid-metabolism-by-directly-regulating-carotenogenic-genes
#1
Su Wen Lu, Yin Zhang, Kaijie Zhu, Wei Yang, Jun Li Ye, Lijun Chai, Qiang Xu, Xiuxin Deng
Although remarkable progress has been made towards understanding carotenoid biosynthesis, the mechanisms that regulate the transcription of carotenogenic genes remain poorly understood. Lycopene β-cyclases (LCYb) are critical enzymes located at the branch point of the carotenoid biosynthetic pathway. Here, we used the promoter sequence of LCYb1 as bait in a yeast one-hybrid screen for promoter-binding proteins from sweet orange (Citrus sinensis). This screen identified a MADS transcription factor, CsMADS6, that was coordinately expressed with fruit development and coloration...
February 20, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29411617/lonb-protease-is-a-novel-regulator-of-carotenogenesis-controlling-degradation-of-phytoene-synthase-in-haloferax-volcanii
#2
Micaela Cerletti, Roberto Paggi, Christian Troetschel, Maria Celeste Ferrari, Carina Ramallo Guevara, Stefan Albaum, Ansgar Poetsch, Rosana De Castro
The membrane protease LonB is an essential protein in the archaeon Haloferax volcanii and globally impacts its physiology. However, natural substrates of the archaeal Lon protease have not been identified. In this study the whole proteome turnover was examined in a H. volcanii LonB mutant under reduced and physiological protease levels. LC-MS/MS combined with stable isotope labeling was applied for the identification/quantitation of membrane and cytoplasm proteins. Differential synthesis and degradation rates were evidenced for 414 proteins in response to Lon expression...
February 7, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29404808/effects-of-different-growth-temperatures-on-growth-development-and-plastid-pigments-metabolism-of-tobacco-nicotiana-tabacum-l-plants
#3
Li Yun Yang, Shuang Long Yang, Jun Ying Li, Jun Hong Ma, Tao Pang, Cong Ming Zou, Bin He, Ming Gong
BACKGROUND: Temperature remarkably affects the growth and metabolism of plants. Tobacco is an important cash crop, and the long-term effects of different growth temperatures (18.5, 23.5 and 28.5 °C, daily average) on growth, development and plastid pigments metabolism of tobacco plants were investigated in this study. RESULTS: Compared with tobacco plants grown under 23.5 °C, treatments with 18.5 and 28.5 °C inhibited the expansion of leaves. The contents of superoxide anion (O 2·- ), hydrogen peroxide (H2O2) and malonaldehyde (MDA) in the leaves were significantly increased under 28...
February 5, 2018: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/29394270/establishment-of-an-arabidopsis-callus-system-to-study-the-interrelations-of-biosynthesis-degradation-and-accumulation-of-carotenoids
#4
Patrick Schaub, Marta Rodriguez-Franco, Christopher Ian Cazzonelli, Daniel Álvarez, Florian Wüst, Ralf Welsch
The net amounts of carotenoids accumulating in plant tissues are determined by the rates of biosynthesis and degradation. While biosynthesis is rate-limited by the activity of PHYTOENE SYNTHASE (PSY), carotenoid losses are caused by catabolic enzymatic and non-enzymatic degradation. We established a system based on non-green Arabidopsis callus which allowed investigating major determinants for high steady-state levels of β-carotene. Wild-type callus development was characterized by strong carotenoid degradation which was only marginally caused by the activity of carotenoid cleavage oxygenases...
2018: PloS One
https://www.readbyqxmd.com/read/29386896/anxiety-and-depression-in-paradoxical-insomnia-a-case-control-study
#5
Jingping Liao, Shichao Zhu, Xiaolin Li
Purpose: To compare anxiety and depression among patients with paradoxical insomnia (Para-I), patients with psychophysiological insomnia (Psy-I), and normal sleepers (NS). Patients and methods: A cross-sectional case-control study was conducted in patients with Para-I (n=63), patients with Psy-I (n=63), and NS (n=63) from southwest China. The three groups were matched for age (mean age: 42.0±10.30 years), gender (per group: male =22; female =41), educational level, and nature of occupation...
2018: Neuropsychiatric Disease and Treatment
https://www.readbyqxmd.com/read/29384872/the-psychological-results-of-438-patients-with-persisting-gerd-symptoms-by-symptom-checklist-90-revised-scl-90-r-questionnaire
#6
Ping Li, Fei Wang, Guo-Zhong Ji, Lin Miao, Sihong You, Xia Chen
Persisting gastroesophageal reflux disease (GERD) symptoms affect mental state and social activities and mental disorders likewise play a crucial role on GERD symptoms. The aim of this study was to analyze the data of Symptom Checklist by 90-Revised (SCL-90-R) questionnaire in patients with persisting GERD symptom and to explore the impact of psychological factors on them.The patients accepted SCL-90-R questionnaire survey, following endoscopy, high-resolution manometry (HRM) and ambulatory impedance-pH monitoring...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29379360/molecular-characterization-of-glucosinolates-and-carotenoid-biosynthetic-genes-in-chinese-cabbage-brassica-rapa-l-ssp-pekinensis
#7
Jin-Hyuk Chun, Na-Hyung Kim, Mi-Suk Seo, Mina Jin, Sang Un Park, Mariadhas Valan Arasu, Sun-Ju Kim, Naif Abdullah Al-Dhabi
The present study aimed to investigate the contents of glucosinolates (GSLs) and carotenoids in eleven varieties of Chinese cabbage in relation to the expression level of the important transcription factors. MS and HPLC analysis identified the presence of 13 GSLs (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, glucocochlearin, glucobrassicanapin, glucoerucin, 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and gluconasturtiin) and four carotenoids (lutein, zeaxanthin, α-carotene and β-carotene)...
January 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29236807/the-ritualization-of-life-and-the-expansion-of-psy-cultures-in-colombia-the-local-and-the-barely-transnational
#8
Carlos Alberto Uribe
This paper exposes the status of psy cultures in Colombia. It is shown how the country's official health system has transformed biomedical psychiatry and cognitive behavioral psychology into the dominant and hegemonic psy culture. However, far from being hegemonic, as presented, both serve to foster and sustain the existence of different "religious" or "sacred" therapeutic systems and practices that denaturalize human existential and psychological suffering. In general, the latter are ritual practices with a strong spiritualist, anti-materialist and antimonist content, that deal freely with a wide range of cosmologies, beliefs and symbols, even including the same concepts and practices of a biomedical origin...
November 2017: História, Ciências, Saúde—Manguinhos
https://www.readbyqxmd.com/read/29236805/psychoanalysis-as-a-belief-system-a-research-program-outline
#9
Mariano Ben Plotkin
This article lays out an agenda for research on the establishment of psy cultures in Latin America. It begins by analyzing some of the debates on the nature of psychoanalysis, a discipline located between the sciences, philosophy and common sense. It argues that the place of psy cultures needs to be problematized as emerging out of modernity in a cultural space like Latin America, where the very concept of modernity differs greatly from that of central countries.
November 2017: História, Ciências, Saúde—Manguinhos
https://www.readbyqxmd.com/read/29216572/gene-expression-profiling-of-astaxanthin-and-fatty-acid-pathways-in-haematococcus-pluvialis-in-response-to-different-led-lighting-conditions
#10
Ruijuan Ma, Skye R Thomas-Hall, Elvis T Chua, Faisal Alsenani, Eladl Eltanahy, Michael E Netzel, Gabriele Netzel, Yinghua Lu, Peer M Schenk
Haematococcus pluvialis is a green microalga of major interest to industry based on its ability to produce large amounts of astaxanthin. Biosynthesis of astaxanthin and its mono- and di-esters was significantly stimulated under 150 μmol m-2 s-1 of white LED (W-150) compared with lower light intensities, but the highest astaxanthin amounts were produced under 70 μmol m-2 s-1 of blue LED (B-70). Transcripts of astaxanthin biosynthesis genes psy, crtO, and bkt2 were upregulated under W-150, while psy, lcy, crtO, and crtR-B were upregulated by B-70...
December 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29201791/psychological-results-of-438-patients-with-persisting-gastroesophageal-reflux-disease-symptoms-by-symptom-checklist-90-revised-questionnaire
#11
Xia Chen, Ping Li, Fei Wang, Guozhong Ji, Lin Miao, Sihong You
Aims and objectives: Gastroesophageal reflux disease (GERD) affects mental state and social activities. On the contrary, mental disorders may also play a crucial role in GERD symptoms. The purpose of the study was to analyze the data of Symptom Checklist 90-Revised (SCL-90-R) questionnaire from patients with persisting GERD and to explore the impact of psychological factors on them. Materials and methods: The patients accepted SCL-90-R questionnaire survey, following endoscopy, high-resolution manometry (HRM), and ambulatory impedance-pH monitoring...
July 2017: Euroasian Journal of Hepato-Gastroenterology
https://www.readbyqxmd.com/read/29182721/molecular-mimicry-modulates-plant-host-responses-to-pathogens
#12
Pamela Ronald, Anna Joe
Background: Pathogens often secrete molecules that mimic those present in the plant host. Recent studies indicate that some of these molecules mimic plant hormones required for development and immunity. Scope and Conclusion: This Viewpoint reviews the literature on microbial molecules produced by plant pathogens that functionally mimic molecules present in the plant host. This article includes examples from nematodes, bacteria and fungi with emphasis on RaxX, a microbial protein produced by the bacterial pathogen Xanthomonas oryzae pv...
November 22, 2017: Annals of Botany
https://www.readbyqxmd.com/read/29172590/the-mediating-effect-of-family-cohesion-in-reducing-patient-symptoms-and-family-distress-in-a-culturally-informed-family-therapy-for-schizophrenia-a-parallel-process-latent-growth-model
#13
Caitlin A Brown, Amy Weisman de Mamani
OBJECTIVE: Although both patients with schizophrenia and their caregivers report elevated levels of depression, anxiety, and stress (DASS), affective symptoms in patients and family members seldom constitute a primary treatment focus. The present study tested whether a culturally informed family therapy for schizophrenia (CIT-S) outperformed standard family psychoeducation (PSY-ED) not only in decreasing patient schizophrenia symptoms, but also in decreasing individual DASS. Because CIT-S fostered family cohesion throughout treatment, we predicted that increases in family cohesion would mediate treatment effects...
November 27, 2017: Journal of Consulting and Clinical Psychology
https://www.readbyqxmd.com/read/29155321/clp-protease-and-or-directly-control-the-proteostasis-of-phytoene-synthase-the-crucial-enzyme-for-carotenoid-biosynthesis-in-arabidopsis
#14
Ralf Welsch, Xiangjun Zhou, Hui Yuan, Daniel Álvarez, Tianhu Sun, Dennis Schlossarek, Yong Yang, Guoxin Shen, Hong Zhang, Manuel Rodriguez-Concepcion, Theodore W Thannhauser, Li Li
Phytoene synthase (PSY) is the crucial plastidial enzyme in the carotenoid biosynthetic pathway. However, its post-translational regulation remains elusive. Likewise, Clp protease constitutes a central part of the plastid protease networks, but its substrates for degradation are not well known. In this study, we report that PSY is a substrate of the Clp protease. PSY was uncovered to physically interact with various Clp protease subunits (i.e. ClpS1, ClpC1, and ClpD). High levels of PSY and several other carotenogenic enzymes over-accumulate in the clpc1, clpp4 and clpr1-2 mutants...
November 16, 2017: Molecular Plant
https://www.readbyqxmd.com/read/29111068/microwave-assisted-synthesis-of-binary-grafted-psyllium-and-its-utility-in-anticancer-formulation
#15
Deepak Kumar, Jyoti Pandey, Pramendra Kumar
A binary grafted copolymer of Psyllium mucilage (Psy) with acrylic acid (AA) and acrylonitrile (An) has been successfully synthesized under microwave conditions for in vitro drug release study. The grafting was confirmed by FTIR spectroscopy, XRD, SEM, EDX, TGA analytical techniques and the intrinsic viscosity study. The swelling behavior of grafted material has been studied in solution of different pH and time. We also prepare Psy-g-Poly (AA-co-An) based beads with anti-cancer drug [(2-Chloro-3-(4-hydroxyphenylamino) naphthalene-1, 4-dione)]...
January 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29109314/a-cross-sectional-study-to-assess-the-perception-of-psychosocial-elements-among-pediatric-patients-visiting-dental-clinics
#16
Ashish Bhalla, U B Rajasekaran, Madhavi Singh, Manish Goutam, Nikhil Grover, Ashok Galav
INTRODUCTION: Children with high dental fear and perception usually encounter high prevalence of dental caries. Data showed that individuals with dental fear will probably defer dental appointments which may result in the formation of a setup of an endless loop of dental fear. Present study was aimed to assess different psycho-social parameters among pediatric patients visiting dental clinics. MATERIALS AND METHODS: In the present cross-sectional study, the data of 200 schoolchildren were collected and analyzed by means of a questionnaire...
November 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/29094166/the-relationship-between-high-order-aberration-and-anterior-ocular-biometry-during-accommodation-in-young-healthy-adults
#17
Bilian Ke, Xinjie Mao, Hong Jiang, Jichang He, Che Liu, Min Li, Ying Yuan, Jianhua Wang
Purpose: This study investigated the anterior ocular anatomic origin of high-order aberration (HOA) components using optical coherence tomography and a Shack-Hartmann wavefront sensor. Methods: A customized system was built to simultaneously capture images of ocular wavefront aberrations and anterior ocular biometry. Relaxed, 2-diopter (D) and 4-D accommodative states were repeatedly measured in 30 young subjects. Custom software was used to correct optical distortions and measure biometric parameters from the images...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29091762/tfcp2-is-required-for-yap-dependent-transcription-to-stimulate-liver-malignancy
#18
Xiao Zhang, Fenyong Sun, Yongxia Qiao, Weisheng Zheng, Ya Liu, Yan Chen, Qi Wu, Xiangfan Liu, Guoqing Zhu, Yuxin Chen, Yongchun Yu, Qiuhui Pan, Jiayi Wang
Although YAP-dependent transcription is closely associated with liver tumorigenesis, the mechanism by which YAP maintains its function is poorly understood. Here, we show that TFCP2 is required for YAP-dependent transcription and liver malignancy. Mechanistically, YAP function is stimulated by TFCP2 via a WW-PSY interaction. TFCP2 also maintains YAP stability by inhibiting βTrCP. Notably, genomic co-occupancy of YAP and TFCP2 is revealed. TFCP2 acts as a transcription co-factor that stimulates YAP transcription by facilitating YAP binding with YAP binding motif (YBF)-containing transcription factors...
October 31, 2017: Cell Reports
https://www.readbyqxmd.com/read/29076614/the-predictive-role-of-health-promoting-behaviors-and-perceived-stress-in-aneurysmal-rupture
#19
Mi-Sun Lee, Chang G Park, Tonda L Hughes, Sang-Eun Jun, Kum Whang, Nahyun Kim
AIM AND OBJECTIVE: We examined the roles of two modifiable factors-health-promoting behaviors (HPB) and perceived stress-in predicting aneurysmal rupture. BACKGROUND: Unruptured intracranial aneurysm (UIA) detection produces significant stress and anxiety in patients because of the risk of rupture. Compared to non-modifiable risk factors for rupture such as age, gender, and aneurysm size/location, less attention has been given to modifiable risk factors. Two modifiable factors, HPBs and perceived stress, have hardly been examined as potential predictors of rupture...
October 27, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28992607/effect-of-uv-radiation-and-its-implications-on-carotenoid-pathway-in-bixa-orellana-l
#20
M Sankari, H Hridya, P Sneha, C George Priya Doss, Siva Ramamoorthy
The current study was undertaken to analyse the effect of short-term UV-B and UV-C radiations in provoking carotenoid biosynthesis in Bixa orellana. Seeds of B. orellana were germinated and exposed to the short term UV pre-treatment under controlled environmental condition for 5days. The UV treated young seedlings response in pigment contents; antioxidant enzyme activity and mRNA gene expression level were analysed. The pigment content such as chlorophyll was increased in both UV-B and UV-C treated seedlings, but the total carotenoid level was decreased when compared to the control seedlings this can be attributed to the plant adaptability to survive in a stressed condition...
October 2, 2017: Journal of Photochemistry and Photobiology. B, Biology
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