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adipocyte differentiation

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https://www.readbyqxmd.com/read/29334696/mast-cells-participate-in-chronic-low-grade-inflammation-within-adipose-tissue
#1
REVIEW
P Żelechowska, J Agier, E Kozłowska, E Brzezińska-Błaszczyk
Obesity is reckoned as one of the civilization diseases, posing a considerable global health issue. Evidence points towards a contribution of multitude immune cell populations in obesity pathomechanism and the development of chronic low-grade inflammation in the expanded adipose tissue. Notably, adipose tissue is a reservoir of mast cells which number in individuals with obesity particularly increased. Some of them tend to degranulation what generate secretion of strong pro-inflammatory and regulatory mediators, as well as cytokines/chemokines...
January 15, 2018: Obesity Reviews: An Official Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29334664/methyl-dehydroabietate-counters-high-fat-diet-induced-insulin-resistance-and-hepatic-steatosis-by-modulating-peroxisome-proliferator-activated-receptor-signaling-in-mice
#2
Hiroki Yoshioka, Yuki Mizuno, Tomohiro Yamaguchi, Yoshimi Ichimaru, Koichi Takeya, Yukio Hitotsuyanagi, Tsunemasa Nonogaki, Yutaka Aoyagi
The aim of this study was to investigate the therapeutic effects of methyl dehydroabietate (mDA) on adipocyte differentiation in 3T3-L1 preadipocytes and obesity characteristics induced by high-fat diet (HFD) in mice. Adipocyte differentiation in 3T3-L1 cells was evaluated after 14 days of incubation with mDA. mDA enhanced adipocyte differentiation in 3T3-L1 cells. For the in vivo evaluation, five-week-old male C57BL/6J mice were fed HFD or normal CE-2 diet (control) for eight weeks. During the experimental period, mice were administered mDA (50 mg/kg, p...
January 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29333383/inhibitory-effects-of-butein-on-adipogenesis-through-upregulation-of-the-nrf2-ho-1-pathway-in-3t3-l1-adipocytes
#3
Jinwoo Yang, Jeehye Sung, Younghwa Kim, Heon Sang Jeong, Junsoo Lee
Butein is reported to have many biological effects, including anti-fibrogenic, anti-cancer, and anti-inflammatory activities. This study investigated the effects of butein on adipocyte differentiation and the Nrf2/heme oxygenase-1 (HO-1) pathway's involvement in its anti adipogenic mechanism. Butein treatment reduced protein expression of key adipogenic transcription factors such as CCAAT-enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). At a concentration of 5, 10, and 25 μM butein, PPARγ was decreased by 78...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29333380/anti-obesity-property-of-lichen-thamnolia-vermicularis-extract-in-3t3-l1-cells-and-diet-induced-obese-mice
#4
Ra-Yeong Choi, Ju Ri Ham, Jiyoung Yeo, Jae-Seoun Hur, Seok-Kyu Park, Myung-Joo Kim, Mi-Kyung Lee
Thamnolia vermicularis (TV) is an edible lichen that is prevalent in the alpine zone of East Asia. This study evaluated the feasibility of using TV acetone extracts as a functional food based on experiments using cell line and obese mice. The cellular triglyceride levels and Oil red O staining of 3T3-L1 cells indicated that TV extracts (5 and 10 μg/mL) dose-dependently suppressed adipocyte differentiation and lipid accumulation compared with the control. The TV extract (0.4%, w/w) in a high-fat diet (HFD) was supplemented to C57BL/6N mice for 12 weeks, and TV extract supplement significantly reduced visceral fat mass and body weight compared with HFD feeding alone...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29324307/integrin-alpha5beta1-suppresses-rbmscs-anoikis-and-promotes-nitric-oxide-production
#5
Hai-Ying Chen, Li Pan, Hong-Li Yang, Peng Xia, Wan-Cheng Yu, Wen-Qiang Tang, Ying-Xin Zhang, Shuang-Feng Chen, Yu-Zeng Xue, Le-Xin Wang
BACKGROUND: Cell based therapy has been heralded as a novel, promising therapeutic strategy for cardiovascular diseases including pulmonary arterial hypertension (PAH). However, the low survival rate after transplantation due to cell death via anoikis is a major obstacle in stem cell therapy. Cells adhesion via Integrin alpha5beta1 (ITGA5B1) has a tendency to exert higher maximum forces. The present study aimed to evaluate the potential protective effect of ITGA5B1 on rat bone marrow mesenchymal stem cells (rBMSCs) from anoikis...
January 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29323267/scaffold-free-generation-of-uniform-adipose-spheroids-for-metabolism-research-and-drug-discovery
#6
Aloysius J Klingelhutz, Francoise A Gourronc, Anna Chaly, David A Wadkins, Anthony J Burand, Kathleen R Markan, Sharon O Idiga, Meng Wu, Matthew J Potthoff, James A Ankrum
Adipose tissue dysfunction is critical to the development of type II diabetes and other metabolic diseases. While monolayer cell culture has been useful for studying fat biology, 2D culture often does not reflect the complexity of fat tissue. Animal models are also problematic in that they are expensive, time consuming, and may not completely recapitulate human biology because of species variation. To address these problems, we have developed a scaffold-free method to generate 3D adipose spheroids from primary or immortal human or mouse pre-adipocytes...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321988/hypolipidemic-effects-of-s-linalool-and-essential-oil-from-cinnamomum-osmophloeum-ct-linalool-leaves-in-mice
#7
Bing-Ho Cheng, Lee-Yan Sheen, Shang-Tzen Chang
Cinnamomum osmophloeum ( tǔ ròu guì) ct. linalool is one of the chemotypes of the indigenous cinnamon in Taiwan. S-(+)-linalool is the major constituent of leaf essential oil (LEO) of C. osmophloeum ct. linalool. This study aimed to investigate its physiological effects including body weight changes, blood biochemical values, and histopathological changes in mice. The mice were treated with LEO, S-(+)-linalool, and R-(-)-linalool. Results demonstrated similar physiological changes in mice treated with LEO and S-(+)-linalool, but significantly different effects in the body weight, TG, TC and blood glucose of R-(-)-linalool group...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29321608/the-effect-of-maternal-vitamin-d-deficiency-during-pregnancy-on-body-fat-and-adipogenesis-in-rat-offspring
#8
Juan Wen, Qin Hong, Xingyun Wang, Lijun Zhu, Tianqi Wu, Pengfei Xu, Ziyi Fu, Lianghui You, Xing Wang, Chenbo Ji, Xirong Guo
To evaluate the effects of maternal vitamin D deficiency on body fat and adipogenesis in offspring rats, and explore the potential mechanism, we constructed a vitamin D deficient rat model and performed metabolic activity evaluation, body fat monitoring, biochemical analysis, adipogenesis assay, methylation microarray and RNA-seq for their offspring rats. We found the weight of vitamin D deficient (VDD) offspring was gradually higher than that of control (CLT) offspring, and the difference was significant since 10 weeks old...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29319174/chronic-high-glucose-and-insulin-stimulate-bone-marrow-stromal-cells-adipogenic-differentiation-in-young-spontaneously-hypertensive-rats
#9
Antonio Hernandes Chaves Neto, Victor Gustavo Balera Brito, Thamine Landim de Barros, Caril Constante Ferreira do Amaral, Dóris Hissako Sumida, Sandra Helena Penha Oliveira
We evaluated whether genetic predisposition is sufficient to induce changes due to chronic high glucose (HG; 25 mmol/L) in the presence or absence of insulin (HGI; 10 µg/mL) on osteogenic differentiation and markers in bone-marrow mesenchymal stem cells (BMSCs) from young Wistar (WBMSCs) and spontaneous hypertensive rats (SBMSCs) without hypertension. HG suppressed osteogenic differentiation in both the strains, observed by mineralization inhibition and decreased levels of the osteogenic markers Runx2, osterix, osteopontin, and bone sialoprotein, compared to osteogenic medium (OM) cells...
January 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29319166/long-non-coding-rna-tcons_00041960-enhances-osteogenesis-and-inhibits-adipogenesis-of-rat-bone-marrow-mesenchymal-stem-cell-by-targeting-mir-204-5p-and-mir-125a-3p
#10
Guowei Shang, Yadong Wang, Yan Xu, Shanfeng Zhang, Xiaoya Sun, Hongya Guan, Xuefeng Zhao, Yisheng Wang, Yuebai Li, Guoqiang Zhao
A growing number of long non-coding RNAs (lncRNAs) have been found to be involved in diverse biological processes such as cell cycle regulation, embryonic development, and cell differentiation. However, limited knowledge is available concerning the underlying mechanisms of lncRNA functions. In this study, we found down-regulation of TCONS_00041960 during adipogenic and osteogenic differentiation of glucocorticoid-treated bone marrow mesenchymal stem cells (BMSCs). Furthermore, up-regulation of TCONS_00041960 promoted expression of osteogenic genes Runx2, osterix, and osteocalcin, and anti-adipogenic gene glucocorticoid-induced leucine zipper (GILZ)...
January 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29316805/red-pepper-seed-inhibits-differentiation-of-3t3-l1-cells-during-the-early-phase-of-adipogenesis-via-the-activation-of-ampk
#11
Hwa-Jin Kim, Mi-Kyoung You, Ziyun Wang, Hyeon-A Kim
Obesity is the main risk factor for metabolic syndromes and there has been an upsurge in demand for effective therapeutic strategies. This study investigated the effect of red pepper seed water extract (RPS) on the process of differentiation in 3T3-L1 adipocytes. RPS treatment significantly suppressed cellular lipid accumulation and reduced the expression of adipocytes-associated proteins, peroxisome proliferator-activated receptor-[Formula: see text] (PPAR-[Formula: see text]), CCAAT/enhancer-binding proteins [Formula: see text] (C/EBP [Formula: see text]), sterol regulatory element binding protein-1c (SREBP-1c), as well as fatty acid synthase (FAS), and fatty acid binding protein 4 (FABP4)...
January 9, 2018: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/29316658/galectin-12-in-cellular-differentiation-apoptosis-and-polarization
#12
REVIEW
Lei Wan, Ri-Yao Yang, Fu-Tong Liu
Galectin-12 is a member of a family of mammalian lectins characterized by their affinity for β-galactosides and consensus amino acid sequences. The protein structure consists of a single polypeptide chain containing two carbohydrate-recognition domains joined by a linker region. Galectin-12 is predominantly expressed in adipose tissue, but is also detected in macrophages and other leukocytes. Downregulation of galectin-12 in mouse 3T3-L1 cells impairs their differentiation into adipocytes. Conversely, overexpression of galectin-12 in vitro induces cell cycle arrest in G1 and apoptosis...
January 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29311623/basal-p53-expression-is-indispensable-for-mesenchymal-stem-cell-integrity
#13
Siddaraju V Boregowda, Veena Krishnappa, Jacqueline Strivelli, Christopher L Haga, Cori N Booker, Donald G Phinney
Marrow-resident mesenchymal stem cells (MSCs) serve as a functional component of the perivascular niche that regulates hematopoiesis. They also represent the main source of bone formed in adult bone marrow, and their bifurcation to osteoblast and adipocyte lineages plays a key role in skeletal homeostasis and aging. Although the tumor suppressor p53 also functions in bone organogenesis, homeostasis, and neoplasia, its role in MSCs remains poorly described. Herein, we examined the normal physiological role of p53 in primary MSCs cultured under physiologic oxygen levels...
January 8, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29311579/structural-basis-for-the-enhanced-anti-diabetic-efficacy-of-lobeglitazone-on-ppar%C3%AE
#14
Jun Young Jang, Hwan Bae, Yong Jae Lee, Young Il Choi, Hyun-Jung Kim, Seung Bum Park, Se Won Suh, Sang Wan Kim, Byung Woo Han
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor superfamily. It functions as a ligand-activated transcription factor and plays important roles in the regulation of adipocyte differentiation, insulin resistance, and inflammation. Here, we report the crystal structures of PPARγ in complex with lobeglitazone, a novel PPARγ agonist, and with rosiglitazone for comparison. The thiazolidinedione (TZD) moiety of lobeglitazone occupies the canonical ligand-binding pocket near the activation function-2 (AF-2) helix (i...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29301300/the-impact-of-lipid-types-and-liposomal-formulations-on-osteoblast-adiposity-and-mineralization
#15
Shun-Fu Chang, Chih-Chang Yeh, Pin-Jyun Chen, Hsin-I Chang
Recent studies have demonstrated that fat accumulation in bone cells is detrimental to bone mass. Both adipocytes and osteoblasts are derived from common multipotent mesenchymal stem cells (MSCs) and hence the presence of fat may increase adipocyte proliferation, differentiation and fat accumulation while inhibiting osteoblast differentiation and bone formation. Lipids are common constituents in supramolecular vesicles (e.g., micelles or liposomes) that serve as drug delivery systems. Liposomal formulations such as Meriva® were proven to decrease joint pain and improve joint function in osteoarthritis (OA) patients...
January 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29298881/multiple-intracellular-signaling-pathways-orchestrate-adipocytic-differentiation-of-human-bone-marrow-stromal-stem-cells
#16
Dalia Ali, Sarah Abuelreich, Nora Alkeraishan, Najla Bin Shwish, Rimi Hamam, Moustapha Kassem, Musaad Alfayez, Abdullah Aldahmash, Nehad M Alajez
Bone marrow adipocyte formation plays a role in bone homeostasis and whole body energy metabolism. However, the transcriptional landscape and signaling pathways associated with adipocyte lineage commitment and maturation are not fully delineated. Thus, we performed global gene expression profiling during adipocyte differentiation of human bone marrow stromal (mesenchymal) stem cells (hMSCs) and identified 2,589 upregulated and 2,583 down regulated mRNA transcripts. Pathway analysis on the upregulated gene list untraveled enrichment in multiple signaling pathways including insulin receptor signaling, focal Adhesion, metapathway  biotransformation, a number of metabolic pathways e...
January 3, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29296822/bone-marrow-sites-differently-imprint-dormancy-and-chemoresistance-to-t-cell-acute-lymphoblastic-leukemia
#17
Xavier Cahu, Julien Calvo, Sandrine Poglio, Nais Prade, Benoit Colsch, Marie-Laure Arcangeli, Thierry Leblanc, Arnaud Petit, Frederic Baleydier, Andre Baruchel, Judith Landman-Parker, Christophe Junot, Jerome Larghero, Paola Ballerini, Eric Delabesse, Benjamin Uzan, Francoise Pflumio
T-cell acute lymphoblastic leukemia (T-ALL) expands in various bone marrow (BM) sites of the body. We investigated whether different BM sites could differently modulate T-ALL propagation using in vivo animal models. We observed that mouse and human T-ALL develop slowly in the BM of tail vertebrae compared with the BM from thorax vertebrae. T-ALL recovered from tail BM displays lower cell-surface marker expression and decreased metabolism and cell-cycle progression, demonstrating a dormancy phenotype. Functionally, tail-derived T-ALL exhibit a deficient short-term ex vivo growth and a delayed in vivo propagation...
September 12, 2017: Blood Advances
https://www.readbyqxmd.com/read/29295544/bee-venom-suppresses-the-differentiation-of-preadipocytes-and-high-fat-diet-induced-obesity-by-inhibiting-adipogenesis
#18
Se-Yun Cheon, Kyung-Sook Chung, Seong-Soo Roh, Yun-Yeop Cha, Hyo-Jin An
Bee venom (BV) has been widely used in the treatment of certain immune-related diseases. It has been used for pain relief and in the treatment of chronic inflammatory diseases. Despite its extensive use, there is little documented evidence to demonstrate its medicinal utility against obesity. In this study, we demonstrated the inhibitory effects of BV on adipocyte differentiation in 3T3-L1 cells and on a high fat diet (HFD)-induced obesity mouse model through the inhibition of adipogenesis. BV inhibited lipid accumulation, visualized by Oil Red O staining, without cytotoxicity in the 3T3-L1 cells...
December 24, 2017: Toxins
https://www.readbyqxmd.com/read/29291086/curcumin-improves-glycolipid-metabolism-through-regulating-peroxisome-proliferator-activated-receptor-%C3%AE-signalling-pathway-in-high-fat-diet-induced-obese-mice-and-3t3-l1-adipocytes
#19
Yanyun Pan, Dandan Zhao, Na Yu, Tian An, Jianan Miao, Fangfang Mo, Yujie Gu, Dongwei Zhang, Sihua Gao, Guangjian Jiang
Curcumin is an active component derived from Curcuma longa L. which is a traditional Chinese medicine that is widely used for treating metabolic diseases through regulating different molecular pathways. Here, in this study, we aimed to comprehensively investigate the effects of curcumin on glycolipid metabolism in vivo and in vitro and then determine the underlying mechanism. Male C57BL/6 J obese mice and 3T3-L1 adipocytes were used for in vivo and in vitro study, respectively. Our results demonstrated that treatment with curcumin for eight weeks decreased body weight, fat mass and serum lipid profiles...
November 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29288588/il-1%C3%AE-inhibits-proliferation-and-adipogenic-differentiation-of-human-adipose-derived-mesenchymal-stem-cells-through-nf-%C3%AE%C2%BAb-and-erk1-2-mediated-proinflammatory-cytokines
#20
Xuerong Sun, Tangbin Zou, Changqing Zuo, Mingmeng Zhang, Benyan Shi, Zhiwen Jiang, Hongjing Cui, Xiaoxin Liao, Xiaoyi Li, Yuelian Tang, Yusheng Liu, Xinguang Liu
Dysfunctional adipogenesis such as subcutaneous lipoatrophy is closely related to insulin resistance and metabolic disorders. Although the expression or release of the cytokine interleukin-1α(L-1α) is known to increase in adipose tissue in response to cell death, cell senescence, aging, or solar radiation, the regulatory role of IL-1α in adipogenesis has not been sufficiently investigated. To investigate the problem, we explored the effect of IL-1α on the proliferation and adipogenic differentiation of human adipose-derived mesenchymal stem cells (ADSCs) using cell counting, alamarBlue assay, oil O staining, Western blot, among others...
December 30, 2017: Cell Biology International
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