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Laser cary

Puneet Kumar, Mridula Goswami, Jatinder Kaur Dhillon, Ferah Rehman, Deepti Thakkar, Kusum Bharti
Background and aims: The aim of the study was to evaluate and compare the surface microhardness and surface morphology of permanent tooth enamel after Er,Cr:YSGG laser irradiation and Fluoride application. Materials and methods: One hundred and twenty premolars extracted for orthodontic purpose were used in the study and randomly divided into 6 groups. Group A was not subjected to any treatment. Group B was subjected to Er,Cr:YSGG laser irradiation. Group C was subjected to Er,Cr:YSGG laser irradiation followed by application of 2% NaF gel for 4 minutes...
October 1, 2016: Laser Therapy
Hirohisa Sato, Yoshimi Niwano, Keisuke Nakamura, Takayuki Mokudai, Hiroyo Ikai, Taro Kanno, Hiroshi Egusa
The present study aimed to evaluate the acute locally injurious property of our most current hydroxyl radical generation system by hydrogen peroxide (H2O2) photolysis. This system, which releases 3% H2O2 with a 405-nm laser, was developed in our laboratory for the treatment of dental and periodontal infectious diseases. First, the hydroxyl radical yield generated by H2O2 photolysis was examined by applying an electron spin resonance-spin trapping technique. Second, the bactericidal effect of the device was examined under a simulant condition in which Streptococcus mutans, a pathogenic bacterial species that causes caries, was irrigated with running 3% H2O2 concomitantly with laser irradiation...
2016: Journal of Toxicological Sciences
M Bizhang, N Wollenweber, P Singh-Hüsgen, G Danesh, S Zimmer
BACKGROUND: The accurate detection of approximal caries is generally difficult. The aim of this study was to assess the ability of the pen-type laser fluorescence device (LF pen) to detect approximal carious lesions in comparison to bitewing radiographs (BW). METHODS: Three hundred forty-one tooth surfaces were diagnosed in 20 patients with an average age of 26.70 (±2.82) years. Each test tooth was sequentially assessed by a single calibrated examiner using visual inspection, BW, and the LF pen...
November 4, 2016: Head & Face Medicine
Maria Inês Rosa, Vilson Sampaio Schambeck, Eduardo Ronconi Dondossola, Maria Cecília Manenti Alexandre, Lisiane Tuon, Antonio Jose Grande, Fernando Hugo
BACKGROUND/OBJECTIVES: The detection of dental caries in the early stages, particularly on the occlusal surfaces, has become a mainstay of contemporary clinical practice. The objective of the study was to verify the accuracy of laser fluorescence (LF) for caries detection. METHODS: A comprehensive search of the MEDLINE (PubMed), EMBase, Cochrane Central Register of Controlled Trials, IBECS, BIOSIS, Web of Science, SCOPUS, Congress Abstracts and Grey literature databases was undertaken from 1980 through January 2016...
September 12, 2016: Journal of Evidence-based Medicine
Gunseli Katirci, R Banu Ermis
BACKGROUND: The aim of this in vitro study was to evaluate the microindentation hardness and chemical composition of residual dentin left at the cavity bottom following removal of carious dentin using the Carisolv chemomechanical and Er:YAG laser caries excavation methods in comparison with the conventional tungsten-carbide bur excavation. METHODS: Sixty-nine extracted permanent teeth with occlusal dentin caries were assigned into three groups according to caries removal technique...
2016: SpringerPlus
Fan Yu, Haohan Yu, Pingting Lin, Yan Dong, Ling Zhang, Xiang Sun, Zhengya Liu, Huihui Guo, Li Huang, Jihua Chen
Resin-based pit-and-fissure sealants are often used to form a barrier on the occlusal surface of molars to treat caries lesions; however, bacteria can remain in the pit and fissures without detection, increasing the risk of secondary caries. Sealants with antimicrobial properties or microbial repellent actions might be advantageous. The aim of this study was to assess the inhibitory effect of a 2-methacryloxylethyl dodecyl methyl ammonium bromide (MAE-DB)-incorporated sealant against Streptococcus mutans. MAE-DB (4% wt) was incorporated into a commercially available sealant, Eco-S resin-based pit-and-fissure sealant (Vericom Co...
2016: PloS One
Alessandro Montedori, Iosief Abraha, Massimiliano Orso, Potito Giuseppe D'Errico, Stefano Pagano, Guido Lombardo
BACKGROUND: Despite considerable improvements in oral health, dental caries continue to be a public health issue. The most frequently used, and universally accepted technique, to remove caries is through mechanical ablation of decayed tissues by means of rotating drills (diamond or tungsten carbide, or both). In the past few decades, the introduction of adhesive filling materials (resin composites) has affected cavity filling procedures by reducing its retention needs, with advantages for dental tissue conservation...
September 26, 2016: Cochrane Database of Systematic Reviews
Zenildo Santos Silva, Ying-Ying Huang, Lucas Freitas de Freitas, Cristiane Miranda França, Sergio Brossi Botta, Patrícia Aparecida Ana, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes, Alessandro Deana, Cintia Raquel Lima Leal, Renato Araujo Prates, Michael R Hamblin, Sandra Kalil Bussadori
This study intended to evaluate the effects of a papain-gel with a red-light absorbing pigment (methylene blue - MB) to mediate photodynamic therapy (PDT) against Streptococcus mutans biofilms. The PapaMBlue was compared with free MB to generate reactive oxygen species using fluorescence probes (SOSG and HPF). PDT (660-nm light) was carried out against S. mutans biofilms grown on either plastic dishes or on collagen membrane and assayed by CFU, live-dead staining using confocal microscopy, transmission electron microscopy and H&E staining for collagen films...
September 19, 2016: Scientific Reports
Bo Liu, Zhihao Yu, Cary Hill, Yujie Cheng, Daniel Homa, Gary Pickrell, Anbo Wang
We present, for the first time to our knowledge, a sapphire-fiber-based distributed high-temperature sensing system based on a Raman distributed sensing technique. High peak power laser pulses at 532 nm were coupled into the sapphire fiber to generate the Raman signal. The returned Raman Stokes and anti-Stokes signals were measured in the time domain to determine the temperature distribution along the fiber. The sensor was demonstrated from room temperature up to 1200°C in which the average standard deviation is about 3...
September 15, 2016: Optics Letters
Wei Zhou, Li-Na Niu, Lin Hu, Kai Jiao, Gang Chang, Li-Juan Shen, Franklin R Tay, Ji-Hua Chen
The present study examined the mechanism for caries resistance and the pulp responses in vital teeth following the use of the augmented-pressure adhesive displacement technique. Dentin adhesives were applied to the surface of sound dentin disks in 4 experimental groups: non-antibacterial adhesive and gentle adhesive displacement (N-G), non-antibacterial adhesive and augmented-pressure adhesive displacement (N-H), antibacterial adhesive and gentle adhesive displacement (A-G), antibacterial adhesive and augmented-pressure adhesive displacement (A-H)...
2016: Scientific Reports
Cary A Shapoff, Jeffrey A Babushkin, David J Wohl
This article discusses the clinical use of laser-microtextured abutments on dental implant restorations. Four cases are presented, each using one of the four commercially available laser-microtextured abutment styles. Numerous preclinical and clinical studies have shown the positive effects of laser microtexturing on the implant platform in limiting crestal bone loss and benefiting soft tissue stability. Other histologic studies of laser microtexturing on the implant abutment have demonstrated the ability of this specific feature to block epithelial downgrowth and provide a functional connective tissue attachment to the abutment surface...
September 2016: International Journal of Periodontics & Restorative Dentistry
Elad Moisseiev, Zeljka Smit-McBride, Sharon Oltjen, Pengfei Zhang, Robert J Zawadzki, Monica Motta, Christopher J Murphy, Whitney Cary, Geralyn Annett, Jan A Nolta, Susanna S Park
PURPOSE: Intravitreal murine lineage-negative bone marrow (BM) hematopoietic cells slow down retinal degeneration. Because human BM CD34+ hematopoietic cells are not precisely comparable to murine cells, this study examined the effect of intravitreal human BM CD34+ cells on the degenerating retina using a murine model. METHODS: C3H/HeJrd1/rd1 mice, immunosuppressed systemically with tacrolimus and rapamycin, were injected intravitreally with PBS (n = 16) or CD34+ cells (n = 16) isolated from human BM using a magnetic cell sorter and labeled with enhanced green fluorescent protein (EGFP)...
August 1, 2016: Investigative Ophthalmology & Visual Science
Xiangyu Sun, Xin Huang, Xu Tan, Yan Si, Xiaozhe Wang, Feng Chen, Shuguo Zheng
BACKGROUND: Severe early childhood caries (s-ECC), which has quite high prevalence among children, is a widespread problem with significant impacts among both developing and developed countries. At present, it is widely known that no early detective techniques and diagnostic tests could have high sensitivity and specificity when using for clinical screening of s-ECC. In this study, we had applied magnetic bead (MB)-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to screen distinctive candidate biomarkers of this disease, so as to establish protein profiles and diagnostic models of s-ECC...
2016: Journal of Translational Medicine
Arash Azizi, Samira Shademan, Maryam Rezai, Arash Rahimi, Shirin Lawaf
BACKGROUND AND OBJECTIVES: Streptococcus mutans (S. mutans) colonizes in the oral cavity and causes dental caries and periodontal diseases. Considering the importance of the treatments that decrease pathogenic microorganisms, the aim of the present research was the assessment of the antimicrobial effect of Photodynamic Therapy (PDT) with Methylene Blue (MB) and Indocyanine Green (IG) photosensitizers on S. mutans. MATERIALS AND METHODS: Sixty four caries-free first premolars were contaminated with 0...
August 10, 2016: Photodiagnosis and Photodynamic Therapy
L Esteban-Tejeda, B Cabal, R Torrecillas, C Prado, E Fernandez-Garcia, R López-Piriz, F Quintero, J Pou, J Penide, J S Moya
Two types of antimicrobial glass fibers containing ZnO and CaO, with diameters ranging from tens of nanometers to 1 µm, were successfully fabricated by a laser spinning technique. The antimicrobial performance was corroborated according to ISO 20743:2013, by using gram-negative (Escherichia coli) and gram-positive (Streptococcus oralis, Streptococcus mutans and Staphylococcus aureus) bacteria, and yeast (Candida krusei) (more than 3 logs of reduction). The metabolic activity and endosomal system of eukaryotic cells were not altered by using eluents of CaO glass submicrometric fibers and ZnO fibers at 1 : 10 dilution as cellular media (viability rates over 70%)...
2016: Biomedical Materials
Han Zhou, Huaibing Liu, Michael D Weir, Mark A Reynolds, Ke Zhang, Hockin H K Xu
OBJECTIVES: Tooth-restoration interfaces are the weak link with secondary caries causing restoration failure. The objectives of this study were to develop an antimicrobial bonding agent with dimethylaminododecyl methacrylate (DMAHDM), and investigate the effects of quaternary amine charge density on three-dimensional (3D) biofilms on dental resin for the first time. METHODS: DMAHDM was synthesized and incorporated into Scotchbond Multi-Purpose bonding agent at mass fractions of 0% (control), 2...
October 2016: Journal of Dentistry
Sabarinathan Niviethitha, Murugan Satta Muthu, Swaminathan Kavitha
OBJECTIVE: To compare the permeability of the enamel of primary teeth from individuals free of Early Childhood Caries (ECC) with that from individuals affected with ECC by assessment of dye penetration using Laser Scanning Confocal Microscope (LSCM). STUDY DESIGN: Experimental in vitro study. Exfoliated primary maxillary anterior teeth (n = 44) were collected and divided into two groups (n=22 per group): samples with ECC (Group 1) and without ECC (Group 2). The samples were immersed in Rhodamine B dye solution for 1 day, cut longitudinally into 3 sections, observed using LSCM...
2016: Journal of Clinical Pediatric Dentistry
Keisuke Nakamura, Midori Shirato, Taro Kanno, Ulf Örtengren, Peter Lingström, Yoshimi Niwano
Prevention of dental caries with maximum conservation of intact tooth substance remains a challenge in dentistry. The present study aimed to evaluate the antimicrobial effect of H2O2 photolysis on Streptococcus mutans biofilm, which may be a novel antimicrobial chemotherapy for treating caries. S. mutans biofilm was grown on disk-shaped hydroxyapatite specimens. After 1-24 h of incubation, growth was assessed by confocal laser scanning microscopy and viable bacterial counting. Resistance to antibiotics (amoxicillin and erythromycin) was evaluated by comparing bactericidal effects on the biofilm with those on planktonic bacteria...
October 2016: International Journal of Antimicrobial Agents
Young-Jae Kim, Sung-Hoon Lee
Dental caries is caused by cariogenic biofilm, an oral biofilm including Streptococcus mutans. Recently, the prevention of dental caries using various probiotics has been attempted. Lactococcus lactis HY 449 is a probiotic bacterium. The aim of this study was to investigate the effect of L. lactis HY 449 on cariogenic biofilm and to analyze inhibitory mechanisms. Cariogenic biofilm was formed in the presence or absence of L. lactis HY 449 and L. lactis ATCC 19435, and analyzed by confocal laser microscope. The formation of cariogenic biofilm was reduced in cultures spiked with both L...
July 19, 2016: Journal of Microbiology and Biotechnology
Kei Horie, Yasushi Shimada, Khairul Matin, Masaomi Ikeda, Alireza Sadr, Yasunori Sumi, Junji Tagami
OBJECTIVES: The aim of this study was to evaluate enamel demineralization at composite restoration margins caused by cariogenic biofilm using swept-source optical coherence tomography (SS-OCT). METHODS: Sixty round-shaped cavities were prepared on the mid-buccal enamel surface of extracted human molars. The cavities were restored with Estelite Flow Quick flowable composite using either Clearfil SE Bond or Clearfil Tri-S Bond ND bonding agents. Streptococcus mutans suspension was applied to form a cariogenic biofilm on the surface...
September 2016: Dental Materials: Official Publication of the Academy of Dental Materials
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