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Laser cary

Luciano G Gavinho, Sidnei A Araujo, Sandra K Bussadori, João V P Silva, Alessandro M Deana
This paper aims to develop a method for laser speckle image segmentation of tooth surfaces for diagnosis of early stages caries. The method, applied directly to a raw image obtained by digital photography, is based on the difference between the speckle pattern of a carious lesion tooth surface area and that of a sound area. Each image is divided into blocks which are identified in a working matrix by their χ2 distance between block histograms of the analyzed image and the reference histograms previously obtained by K-means from healthy (h_Sound) and lesioned (h_Decay) areas, separately...
May 5, 2018: Lasers in Medical Science
Tisson V Job, Girish T Narayana, Kishan K Venkappa, K Binu Nathan, Shameem Ahsan, Jayakkodi Harikaran
Aim: The aim of this study was to compare the remineralization potential of three different dentifrices using Raman spectroscopy and confocal laser scanning microscopy (CLSM). Materials and methods: Totally, 30 extracted intact impacted third molar teeth were selected and the crown of each tooth in a group was separated from the root and longitudinally sectioned into four parts with each section under a subgroup, of which one section was an untreated section, the second and the third sections were demineralized in a demineralizing solution, and the third section was remineralized after demineralization...
April 1, 2018: Journal of Contemporary Dental Practice
Danilo Augusto de Holanda Ferreira, Carolina Patrícia Aires, Regina Célia Bressan Queiroz De Figueiredo, Frederico Barbosa de Sousa
The amount of organic material in the cariogenic environment correlates with the amount of organic material incorporated in carious enamel. The incorporated organic material may be expected to reduce the pore volumes available for remineralization and resin infiltration, but these expected outcomes have not yet been quantified. We tested the effect of the amount of organic content in the cariogenic agent on remineralization and the resin-occluded pore volume in artificial subsurface enamel caries. An acid gel (organic-rich; G1) and an aqueous solution (organic-poor; G2) were used to induce subsurface lesions in human enamel...
May 3, 2018: Caries Research
Siying Tao, Yan Zhu, He Yuan, Sibei Tao, Yiming Cheng, Jiyao Li, Libang He
The study aimed to evaluate the efficacy of the combination of CPP-ACP and fluorides compared with fluorides monotherapy on patients with early caries lesions. The Medline, Embase and Cochrane databases up to August 2017 were scanned, with no restrictions. Studies satisfied the guideline of randomised controlled trials (RCTs), the patients with early caries lesions and data considering the efficacy of fluorides and CPP-ACP versus fluorides alone were selected. There was no language restriction during the literature search process, however, only papers in English or Chinese were included during the selection process...
2018: PloS One
Ollie Yiru Yu, Irene Shuping Zhao, May Lei Mei, Edward Chin-Man Lo, Chun-Hung Chu
This study investigated the effect of 25% silver nitrate (AgNO₃) and 5% sodium fluoride (NaF) varnish with functionalized tri-calcium phosphate (fTCP) on a Streptococcus mutans ( S. mutans ) biofilm and dentine caries lesion. Demineralised dentine specimens were treated with 25% AgNO₃ and 5% NaF + fTCP (Group 1), 25% AgNO₃ and 5% NaF (Group 2), 25% AgNO₃ (Group 3), or water (Group 4). The specimens were subjected to a S. mutans biofilm challenge after treatment. The biofilm was then studied via scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony forming units (CFU)...
April 25, 2018: International Journal of Molecular Sciences
Bingchun Li, Xinlong Li, Huancai Lin, Yan Zhou
Streptococcus mutans (S. mutans) has been proved to be the main aetiological factor in dental caries. Curcumin, a natural product, has been shown to exhibit therapeutic antibacterial activity, suggesting that curcumin may be of clinical interest. The objective of this study is to evaluate the inhibitory effects of curcumin on metabolism and biofilm formation in S. mutans using a vitro biofilm model in an artificial oral environment. S. mutans biofilms were treated with varying concentrations of curcumin. The biofilm metabolism and biofilm biomass were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and the crystal violet assay...
2018: BioMed Research International
Minh-Huy Dang, Ji-Eun Jung, Hyeon-Mi Choi, Jae-Gyu Jeon
The aim of this study was to investigate the difference in composition and virulence of Streptococcus mutans biofilms according to substratum direction. S. mutans biofilms (46-h-old) were formed on three different saliva-coated hydroxyapatite (sHA) disc direction groups: downward (discs placed in the direction of gravity), vertical (discs placed parallel to gravity direction), and upward (discs placed opposite to gravity). The 46-h-old biofilms on sHA discs in the upward direction showed the highest biofilm accumulation, colony forming unit (CFU) count, and extracellular polysaccharide (EPS) amount, followed by those in the vertical and downward directions...
April 19, 2018: Scientific Reports
Nicholas G Brown, Joel Camilo, Eric Nordstrom, Roy D Yen, Norio Fukami, Brian C Brauer, Sachin Wani, Stuart K Amateau, Augustin R Attwell, Raj J Shah
OBJECTIVES: Advanced ERCP techniques (AETs) for difficult biliary stones include peroral cholangioscopy (POC) with electrohydraulic/laser lithotripsy (EHL/LL), endoscopic papillary large balloon dilation (EPLBD) and mechanical lithotripsy (ML). We assess the efficacy of AETs. METHODS: A retrospective query for AETs. PRIMARY OUTCOME: Complete duct clearance. Secondary outcome: Complete duct clearance by technique. Statistical Analysis version 9...
April 12, 2018: Scandinavian Journal of Gastroenterology
Franziska Hetrodt, Julian Lausch, Hendrik Meyer-Lueckel, Christian Apel, Georg Conrads
OBJECTIVE: Two factors for secondary caries formation were evaluated using an artificial biofilm model, saliva as additive in culture medium and bonding procedures of composite materials for artificial gap creation. DESIGN: Standardized cavities were prepared in bovine tooth samples (n = 44), treated with two different bonding pretreatments, restored and after artificial ageing incubated with Streptococcus mutans in a Mueller-Hinton-Broth-Sugar medium with or without human saliva for seven days...
March 27, 2018: Archives of Oral Biology
Cary Presser, Ashot Nazarian, Amit Millo
Thermochemical characteristics were determined for several National Institute of Standards and Technology standard-reference-material petroleum and biodiesel fuels, using a novel laser-heating calorimetry technique. Measurements focused on the sample thermal behavior, specific heat release rate, and total specific heat release. The experimental apparatus consists of a copper sphere-shaped reactor mounted within a chamber, along with laser-beam-steering optical components, gas-supply manifold, and a computer-controlled data-acquisition system...
February 2018: Fuel
Romina Brignardello-Petersen
No abstract text is available yet for this article.
February 23, 2018: Journal of the American Dental Association
Albert Ngo, Kenneth H Chan, Oanh Le, Jacob C Simon, Daniel Fried
Recent studies have shown that near-IR (NIR) imaging methods such as NIR reflectance can be used to image lesions on proximal surfaces, and optical coherence tomography (OCT) can be used to measure the depth of those lesions below the tooth surface. These imaging modalities can be used to acquire high contrast images of demineralized tooth surfaces, and 2-D and 3-D images can be extracted from this data. At NIR wavelengths longer than 1200-nm, there is no interference from stains and the contrast is only due to the increased light scattering of the demineralization...
January 2018: Proceedings of SPIE
Wenling Zhang, Xiaohong Deng, Xuedong Zhou, Yuqing Hao, Yuqing Li
Dental caries is a chronic progressive disease occurring in the tooth hard tissue due to multiple factors, in which bacteria are the initial cause. Both Streptococcus mutans and Streptococcus sanguinis are main members of oral biofilm. Helicobacter pylori may also be detected in dental plaque, playing an important role in the development of dental caries. OBJECTIVE: The aim of this study was to investigate the effect of H. pylori culture supernatant on S. mutans and S. sanguinis dual-species biofilm and to evaluate its potential ability on affecting dental health...
2018: Journal of Applied Oral Science: Revista FOB
Diana Mortensen, Ilse Hessing-Olsen, Kim Rud Ekstrand, Svante Twetman
OBJECTIVE: To evaluate the clinical performance of impedance spectroscopy, laser fluorescence, and bitewing radiographs in detecting occlusal caries and compare them with visual scores. METHOD AND MATERIALS: In 62 adults, one occlusal surface per person was selected and independently examined by two examiners using the visual ICDAS scoring system, CarieScan PRO (ACIS), DIAGNOdent pen (LF-pen), and bitewing radiographs. The procedures were repeated within 1 to 4 weeks...
2018: Quintessence International
Banu Öter, Nursen Topçuog Lu, Mehmet K Tank, Sevi Burçak Çehreli
BACKGROUND: A successful primary root canal treatment depends on effective shaping and cleaning the root canal system and finally filling it with a hermetic sealer. Clinically, roots of primary teeth are difficult to shape and the irrigation/disinfection protocol has great importance on prognosis. OBJECTIVE: The present study evaluated the antibacterial efficiency of Endosafe (Orangedental GmbH & Co. KG), photo-activated disinfection (PAD; Orangedental GmbH & Co...
April 2018: Photomedicine and Laser Surgery
Kaihua Sun, Ling Yuan, Zhonghua Shen, Qingping Zhu
This study aims to evaluate early caries in human teeth with different geometry structures by using the dispersion curves of a surface acoustic wave (SAW) generated by a pulsed laser. Through the finite element method, SAWs propagating on teeth models with different enamel thickness and curvature radius were simulated, and the influence of the geometry difference on the dispersion curves of SAWs was discussed. Laser ultrasonic experiments were performed on an extracted human incisor and molar with different demineralization conditions...
February 10, 2018: Applied Optics
H S Sancakli, S H Siso, S O Yildiz, Y B Gökçe
Objective: The aim of this study was to evaluate the antibacterial surface pretreatment methods against Streptococcus mutans within the infected dentin surface using a tooth cavity model. Material and Methods: Seventy-two cavities were prepared on caries-free third molars (n = 8). After sterilization, teeth were inoculated with S. mutans for 48 h. One cavity of each tooth was used to evaluate the infection. Following inoculation, infected cavity surfaces were treated either with (1) Er:YAG Laser (1W; 5x5s, Smart 2940D Plus, Deka Laser), (2) Ozone (80s; HealOzone, Kavo), (3) ErYAG-Ozone combination, (4) Er:YAG-Ozone-CHX combination, (5) Chlorhexidine (CHX), (6) Clearfil Protect Bond (PB), (7) potassium-titanyl-phosphate (KTP) Laser (1W; 60 s, SMARTLITE D, Deka Laser), (8) KTP-Ozone combination, and (9) KTP-Ozone-CHX...
February 2018: Nigerian Journal of Clinical Practice
Yin-Lin Wang, Hao-Hueng Chang, Yu-Chih Chiang, Yu-Chen Lu, Chun-Pin Lin
BACKGROUND/PURPOSE: Fluoride and epigallocatechin gallate (EGCG) have been proven to prevent dental caries. The purpose of this study was to evaluate the effects of fluoride and EGCG on soft-drink-induced dental erosion in vitro. METHODS: Forty enamel and dentin specimens were prepared from extracted human teeth. The specimens were divided into 4 groups and treated separately with distilled water (as control), 0.5 M sodium fluoride (NF), 400 μM EGCG (EG), and a solution containing 0...
April 2018: Journal of the Formosan Medical Association, Taiwan Yi Zhi
Lijun Huo, Xiangya Huang, Junqi Ling, Hongyan Liu, Jia Liu
The present study aimed to design, synthesize and screen specifically targeted antimicrobial peptides (STAMPs) that can selectively kill Streptococcus mutans ( S. mutans ) in the biofilm, and to detect protein metabolism, in order to investigate the mechanism of the antibacterial functions of STAMPs against S. mutans . A series of STAMPs were synthesized, and their effects on the selective antibacterial activity of S. mutans on single species and multi-species biofilms under the condition of the planktonic state were studied...
February 2018: Experimental and Therapeutic Medicine
Hyun-Jun Yoo, Su-Kyung Jwa
OBJECTIVE: The biofilm of Streptococcus mutans is associated with induction of dental caries. Also, they produce glucan as an extracellular polysaccharide through glucosyltransferases and help the formation of cariogenic biofilm. β-caryophyllene has been used for therapeutic agent in traditional medicine and has antimicrobial activity. The purpose of this study was to investigate the effect of β-caryophyllene on S. mutans biofilm and the expression of biofilm-related factor. DESIGN: The susceptibility assay of S...
April 2018: Archives of Oral Biology
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