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https://www.readbyqxmd.com/read/28549260/identification-of-oral-cavity-biofilm-forming-bacteria-and-determination-of-their-growth-inhibition-by-acacia-arabica-tamarix-aphylla-l-and-melia-azedarach-l-medicinal-plants
#1
Muhammad Khalid, Danial Hassani, Muhammad Bilal, Zahid Ali Butt, Muhammad Hamayun, Ayaz Ahmad, Danfeng Huang, Anwar Hussain
OBJECTIVE: Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains. METHODS: Pathogenic bacteria from dental biofilms were isolated, cultured, identified by phylogenetic analysis using PCR-based 16S ribosomal RNA (or 16S rRNA) nucleotide sequences and were analyzed for their biofilm forming capability...
May 18, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28542863/mechanisms-of-silver-diamine-fluoride-on-arresting-caries-a-literature-review
#2
REVIEW
Irene Shuping Zhao, Sherry Shiqian Gao, Noriko Hiraishi, Michael Francis Burrow, Duangporn Duangthip, May Lei Mei, Edward Chin-Man Lo, Chun-Hung Chu
OBJECTIVE: To review the evidence regarding the mechanisms of silver diamine fluoride (SDF) for arresting caries. METHODS: A literature search was conducted using the keywords silver diamine fluoride, and its alternative names, in seven databases: PubMed, Embase and Scopus (English); China National Knowledge Infrastructure (Chinese); Bilioteca Virtual em Saude (Portuguese); Biblioteca Virtual en Salud Espana (Spanish); and Ichushi-Web (Japanese). The titles and abstracts were screened...
May 21, 2017: International Dental Journal
https://www.readbyqxmd.com/read/28536781/oral-health-status-in-adult-patients-with-newly-diagnosed-acute-leukemia
#3
Rilana Busjan, Justin Hasenkamp, Gerhard Schmalz, Rainer Haak, Lorenz Trümper, Dirk Ziebolz
BACKGROUND: The aim of this cross-sectional study was to evaluate the oral health of adult patients with newly diagnosed acute leukemia. METHODS: Patients with initially diagnosed acute myeloid (AML) or lymphocytic (ALL) leukemia and a matched healthy control (HC) group were included. The oral investigation comprised inspection of the oral mucosa; the decayed (D), missing (M), and filled (F) teeth (DMF-T) index; and a detailed periodontal status. Subgingival biofilm samples were analyzed (polymerase chain reaction) for the presence of selected potentially periodontal pathogenic bacteria...
May 23, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28534987/high%C3%A2-throughput-sequencing-analyses-of-oral-microbial-diversity-in-healthy-people-and-patients-with-dental-caries-and-periodontal-disease
#4
Tingtao Chen, Yan Shi, Xiaolei Wang, Xin Wang, Fanjing Meng, Shaoguo Yang, Jian Yang, Hongbo Xin
Recurrence of oral diseases caused by antibiotics has brought about an urgent requirement to explore the oral microbial diversity in the human oral cavity. In the present study, the high‑throughput sequencing method was adopted to compare the microbial diversity of healthy people and oral patients and sequence analysis was performed by UPARSE software package. The Venn results indicated that a mean of 315 operational taxonomic units (OTUs) was obtained, and 73, 64, 53, 19 and 18 common OTUs belonging to Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria, respectively, were identified in healthy people...
May 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534286/development-of-a-novel-resin-based-dental-material-with-dual-biocidal-modes-and-sustained-release-of-ag-ions-based-on-photocurable-core-shell-agbr-cationic-polymer-nanocomposites
#5
Weiwei Cao, Yu Zhang, Xi Wang, Yinyan Chen, Qiang Li, Xiaodong Xing, Yuhong Xiao, Xuefeng Peng, Zhiwen Ye
Research on the incorporation of cutting-edge nano-antibacterial agent for designing dental materials with potent and long-lasting antibacterial property is demanding and provoking work. In this study, a novel resin-based dental material containing photocurable core-shell AgBr/cationic polymer nanocomposite (AgBr/BHPVP) was designed and developed. The shell of polymerizable cationic polymer not only provided non-releasing antibacterial capability for dental resins, but also had the potential to polymerize with other methacrylate monomers and prevented nanoparticles from aggregating in the resin matrix...
July 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28530844/developing-a-new-generation-of-antimicrobial-and-bioactive-dental-resins
#6
L Cheng, K Zhang, N Zhang, M A S Melo, M D Weir, X D Zhou, Y X Bai, M A Reynolds, H H K Xu
Dental caries is prevalent, and secondary caries causes restoration failures. This article reviews recent studies on developing a new generation of bioactive resins with anticaries properties. Extensive effects were made to develop new antimicrobial composites, bonding agents, and other resins containing quaternary ammonium methacrylates to suppress plaque buildup and bacterial acid production. The effects of alkyl chain length and charge density and the antimicrobial mechanisms for chlorhexidine, nano-silver, quaternary ammonium methacrylates, and protein-repellent agents were discussed...
May 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28526785/effects-of-arginine-on-growth-virulence-gene-expression-and-stress-tolerance-by-streptococcus-mutans
#7
Brinta Chakraborty, Robert A Burne
Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In contrast, many commensal bacteria produce ammonia through the arginine deiminase system (ADS), which moderates the pH of oral biofilms. Arginine metabolism by the ADS is a significant deterrent to the initiation and progression of dental caries...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28507831/streptococcus%C3%A2-gordonii%C3%A2-empyema-a-case-report-and-review-of-empyema
#8
Amanda M Krantz, Felicia Ratnaraj, Manasa Velagapudi, Mridula Krishnan, Nagarjuna R Gujjula, Pamela A Foral, Laurel Preheim
Streptococcus gordonii (S. gordonii) is a pioneer oral bacteria that is recognized as an agent of bacterial endocarditis. However, an extensive review of the literature revealed no reported case of S. gordonii causing empyema. We present a case of a 65-year-old male who presented with respiratory distress. Physical examination revealed several dental caries with decreased breath sounds in the bibasilar regions. A computed tomography (CT) scan of the chest and abdomen demonstrated left-sided pleural effusion and a 4...
April 12, 2017: Curēus
https://www.readbyqxmd.com/read/28502123/novel-method-for-the-depletion-of-cariogenic-bacteria-using-dextranomer-microspheres
#9
Lauren Mashburn-Warren, Jennifer S Downey, Steven D Goodman
Streptococcus mutans is recognized as one of the key contributors to the dysbiotic state that results in dental caries. While existing treatment strategies reduce the incidence of tooth decay, they also eliminate both the cariogenic and beneficial microbes. Here we introduce a novel treatment alternative using Sephadex, cross-linked dextranomer microspheres (DMs), typically used for gel filtration chromatography. In addition DM beads can be used for affinity purification of glucosyltransferases (GTFs) from S...
May 14, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28492194/comparison-of-the-streptococcus-mutans-and-lactobacillus-colony-count-changes-in-saliva-following-chlorhexidine-0-12-mouth-rinse-combination-mouth-rinse-and-green-tea-extract-0-5-mouth-rinse-in-children
#10
Rahul J Hegde, Shamika Kamath
BACKGROUND: Compounds present in green tea have been shown to inhibit the growth and activity of bacteria associated with oral infections. The purpose of this study was to compare the efficacy of chlorhexidine (0.12%) mouth rinse and combination (chlorhexidine and sodium fluoride) mouth rinse to that of green tea extract (0.5%) mouth rinse in reducing the salivary count of Streptococcus mutans and Lactobacillus in children. MATERIALS AND METHODS: The sample for the study consisted of 75 school children aged 8-12 years with four or more (decay component) of decayed, missing, and filled teeth index...
April 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28466982/introduction-to-proceedings-of-healthy-futures-engaging-the-oral-health-community-in-childhood-obesity-prevention-national-conference
#11
Norman Tinanoff, Katrina Holt
OBJECTIVES: The Robert Wood Johnson Foundation (RWJF) has worked to ensure that all children have healthy weights. To promote this goal, the RWJF has supported the Healthy Futures: Engaging the Oral Health Community in Childhood Obesity Prevention National Conference, held on November 3-4, 2016, and the proceeding of this conference. The goals of the conference were to increase understanding of the science focusing on oral health and childhood obesity, increase understanding of how to prevent childhood obesity, and provide opportunities to network and plan activities to prevent childhood obesity...
May 3, 2017: Journal of Public Health Dentistry
https://www.readbyqxmd.com/read/28462177/infant-and-child-oral-health-risk-status-correlated-to-behavioral-habits-of-parents-or-caregivers-a-survey-in-central-italy
#12
Iole Vozza, Francesca Capasso, Elisa Marrese, Antonella Polimeni, Livia Ottolenghi
AIM: The aim of this survey was to evaluate the knowledge and awareness of parents and caregivers about potential oral health risk factors for their children in their first months of life (3-30 months). MATERIALS AND METHODS: The participation to the survey was proposed to all parents or caregivers of children attending the public consulting service in Latina for mandatory vaccinations during the period of June to August 2014. A self-administered questionnaire was completed to obtain information regarding demographic variables, infant feeding practice, maternal oral health during and after pregnancy, children's oral hygiene habits and risk behaviors (e...
March 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28461867/comparison-of-the-effect-of-resin-infiltrant-fluoride-varnish-and-nano-hydroxy-apatite-paste-on-surface-hardness-and-streptococcus-mutans-adhesion-to-artificial-enamel-lesions
#13
Mahdiye Aziznezhad, Homayoon Alaghemand, Zahra Shahande, Nilgoon Pasdar, Ali Bijani, Abdolreza Eslami, Zohre Dastan
INTRODUCTION: Dental caries is a major public health problem, and Streptococcus mutans is considered the main causal agent of dental caries. This study aimed to compare the effect of three re-mineralizing materials: resin infiltrant, fluoride varnish, and nano-hydroxy apatite paste on the surface hardness and adhesion of Streptococcus mutans as noninvasive treatments for initial enamel lesions. METHODS: This experimental study was conducted from December 2015 through March 2016 in Babol, Iran...
March 2017: Electronic Physician
https://www.readbyqxmd.com/read/28407420/dental-biofilm-infections-an-update
#14
REVIEW
Tove Larsen, Nils-Erik Fiehn
Teeth are colonized by oral bacteria from saliva containing more than 700 different bacterial species. If removed regularly, the dental biofilm mainly comprises oral streptococci and is regarded as resident microflora. But if left undisturbed, a complex biofilm containing up to 100 bacterial species at a site will build up and may eventually cause development of disease. Depending on local ecological factors, the composition of the dental biofilm may vary considerably. With access to excess carbohydrates, the dental biofilm will be dominated by mainly gram-positive carbohydrate-fermenting bacteria causing demineralization of teeth, dental caries, which may further lead to inflammation and necrosis in the pulp and periapical region, i...
April 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28403796/riboswitches-as-potential-targets-for-the-development-of-anti-biofilm-drugs
#15
José A Reyes-Darias, Tino Krell
In nature, bacteria can exist as single motile cells or as sessile cellular community, known as microbial biofilms. Bacteria within biofilms are embedded in a self-produced extracellular matrix that makes them more resistant to antibiotic treatment and responses of the host immune system. Microbial biofilms are very important in medicine since they are associated with several human diseases such as dental caries, periodontitis, otitis media, infective endocarditis, infectious kidney stones, osteomyelitis or prostatitis...
April 7, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28377661/awareness-of-lebanese-pediatricians-regarding-children-s-oral-health
#16
Nahla Nassif, Balsam Noueiri, Riad Bacho, Kassem Kassak
Pediatricians have an important role in early identification and prevention of oral health, but they reported lack of knowledge in this field. AIM: the aim of the study is to evaluate the knowledge and attitudes of Lebanese pediatricians regarding children's oral health. MATERIALS AND METHODS: A cross sectional study including 100 Lebanese pediatricians was performed. They answered 21 questions. Three variables were taken into consideration: The number of years in practice, the place and type of practice...
January 2017: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28372291/current-challenges-in-human-tooth-revitalization
#17
Maxime Ducret, Hugo Fabre, Alexis Celle, Frédéric Mallein-Gerin, Emeline Perrier-Groult, Brigitte Alliot-Licht, Jean-Christophe Farges
Tooth vitality and health are related to the presence of a living connective tissue, the dental pulp (DP), in the center of the dental organ. The DP contains the tooth immune defence system that is activated against invading oral cariogenic bacteria during the caries process and the tissue repair/regeneration machinery involved following microorganisms' eradication. However, penetration of oral bacteria into the DP often leads to complete tissue destruction and colonization of the endodontic space by microorganisms...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28367914/plasma-treated-water-eliminates-streptococcus-mutans-in-infected-dentin-model
#18
Tatsuya Tasaki, Tomoko Ohshima, Emi Usui, Satoshi Ikawa, Katsuhisa Kitano, Nobuko Maeda, Yasuko Momoi
Non-mechanical procedures for removing caries-infected dentin are warranted in dentistry. We previously demonstrated the marked sterilization effect for direct irradiation of low-temperature plasma using dentin model infected with Streptococcus mutans. However it requires 180 s of intraoral plasma irradiation to eliminate bacteria. We alternatively investigated whether plasma-treated water (PTW), i.e., pure water exposed to plasma in an atmosphere, has a same bactericidal activity with the plasma irradiation...
March 29, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28358306/effect-of-the-biofilm-age-and-starvation-on-acid-tolerance-of-biofilm-formed-by-streptococcus-mutans-isolated-from-caries-active-and-caries-free-adults
#19
Shan Jiang, Shuai Chen, Chengfei Zhang, Xingfu Zhao, Xiaojing Huang, Zhiyu Cai
Streptococcus mutans (S. mutans) is considered a leading cause of dental caries. The capability of S. mutans to tolerate low pH is essential for its cariogenicity. Aciduricity of S. mutans is linked to its adaptation to environmental stress in oral cavity. This study aimed to investigate the effect of biofilm age and starvation condition on acid tolerance of biofilm formed by S. mutans clinical isolates. S. mutans clinical strains isolated from caries-active (SM593) and caries-free (SM18) adults and a reference strain (ATCC25175) were used for biofilm formation...
March 30, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28358034/in-silico-identification-of-potential-inhibitors-targeting-streptococcus-mutans-sortase-a
#20
Hao Luo, Dan-Feng Liang, Min-Yue Bao, Rong Sun, Yuan-Yuan Li, Jian-Zong Li, Xin Wang, Kai-Min Lu, Jin-Ku Bao
Dental caries is one of the most common chronic diseases and is caused by acid fermentation of bacteria adhered to the teeth. Streptococcus mutans (S. mutans) utilizes sortase A (SrtA) to anchor surface proteins to the cell wall and forms a biofilm to facilitate its adhesion to the tooth surface. Some plant natural products, especially several flavonoids, are effective inhibitors of SrtA. However, given the limited number of inhibitors and the development of drug resistance, the discovery of new inhibitors is urgent...
March 2017: International Journal of Oral Science
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