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https://www.readbyqxmd.com/read/29340524/efficacy-of-removal-of-cariogenic-bacteria-and-carious-dentin-by-ablation-using-different-modes-of-er-yag-lasers
#1
A Baraba, L Kqiku, D Gabrić, Ž Verzak, K Hanscho, I Miletić
The primary objective of this in vitro study was to evaluate the efficiency of removal of cariogenic bacteria and carious dentin by ablation using two lasers: fluorescence-feedback controlled (FFC) Er:YAG laser and different pulses of Er:YAG laser based on variable square pulse technology (VSPt). The secondary objective was to measure the temperature during laser ablation of carious tissue. Seventy-two extracted human molars were used in this study. Sixty teeth with carious dentin were randomly divided into four experimental groups according to the treatment for caries removal: group 1: 400 µs (FFC group); group 2: super short pulse (SSP group, 50 µs pulse); group 3: medium short pulse (MSP group, 100 µs pulse); group 4: short pulse (SP group, 300 µs pulse) and one positive control group with no treatment...
January 11, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29340299/prospective-study-of-the-feasibility-of-point-of-care-testing-strategy-for-carbapenem-resistant-organism-detection
#2
Rahul Pannala, Bruce Baldwin, Vijay Aluru, Thomas E Grys, Jordan Holmes, Laurence J Miller, M Edwyn Harrison, Cuong C Nguyen, Fred C Tenover, David Persing, Douglas O Faigel
Background/aims:  In an investigator-initiated, prospective study, we evaluated the feasibility of a five-gene sequence point-of-care (POC) testing strategy (Xpert CARBA-R Assay, Cepheid Inc., Sunnyvale, CA, USA), compared to reference laboratory PCR (48 - 72 hours turnaround time, two gene sequences), in patients undergoing endoscopic retrograde cholangiopancreatography (ERCP) and in a hospital outbreak investigation. Methods:  After informed consent, patients undergoing ERCP (September 2015 - April 2016, n = 191) at Mayo Clinic and potential hospital contacts (n = 9) of an index carbapenem-resistant organism (CRO)-positive inpatient were included...
January 2018: Endoscopy International Open
https://www.readbyqxmd.com/read/29337678/salivary-scd14-as-a-potential-biomarker-of-dental-caries-activity-in-adults
#3
Ljerka Prester, Nives Protrka, Jelena Macan, Marina Katunarić
CD14 is a co-receptor involved in the recognition of Gram-negative and Gram-positive bacteria, the latter known to cause dental caries. The aim of this study was to determine whether soluble CD14 (sCD14) in saliva was associated with caries activity and the collection method from the saliva. The study included 55 participants aged 20 to 40 years, 30 with dental caries and 25 caries-free controls. We collected 110 saliva samples in total, 55 of resting saliva and 55 of mechanically stimulated saliva. Median levels of sCD14, measured with a matrix-matched enzyme-linked immunosorbent assay (ELISA), were higher in the caries-active than in the caries-free group in either resting (203...
December 20, 2017: Arhiv za Higijenu Rada i Toksikologiju
https://www.readbyqxmd.com/read/29335687/structure-of-the-bacterial-community-in-different-stages-of-early-childhood-caries
#4
Marcos Ximenes, Rafael Dutra de Armas, Thaisa Cezária Triches, Mariane Cardoso, Ricardo de Souza Vieira
PURPOSE: To characterise in vivo the structure of bacterial communities in decayed and sound primary teeth. MATERIALS AND METHODS: Samples of biofilms were collected from three groups of patients with complete and exclusively primary dentition (n = 45): G1: sound teeth (n = 15); G2: enamel lesion (n = 15); G3: dentin lesion (n = 15). DNA was extracted (CTAB 2%) from the biofilm, the partial 16S rRNA gene was amplified with Bacteria Universal Primers (BA338fGC - UN518r) and subjected to DGGE (denaturing gradient gel electrophoresis)...
January 15, 2018: Oral Health & Preventive Dentistry
https://www.readbyqxmd.com/read/29327482/streptococcus-oralis-maintains-homeostasis-in-supragingival-biofilms-by-antagonizing-cariogenic-pathogen-streptococcus-mutans
#5
Thomas Thurnheer, Georgios N Belibasakis
Bacteria residing in oral biofilms live in a state of dynamic equilibrium with one another. The intricate synergistic or antagonistic interactions between them are crucial for determining this balance. Using the 6-species Zürich "supragingival" biofilm model, this study aimed to investigate interactions regarding growth and localization of the constituent species. As control, an inoculum containing all six strains was used, whereas in each of the further five inocula one of the bacterial species was absent, and in the last both streptococci were absent...
January 11, 2018: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/29326510/isolation-and-typing-of-streptococcus-mutans-and-streptococcus-sobrinus-from-caries-active-subjects
#6
Hamzah Abdulrahman Salman, R Senthilkumar, Khalid Imran, K Panneer Selvam
Background: Streptococcus mutans and Streptococcus sobrinus are main etiological agents of dental caries. Aim: The aim of the study was to isolate, identify, characterize, and determine the minimum inhibitory concentration (MIC) of S. mutans and S. sobrinus from caries-active subjects. Materials and Methods: Sixty-five plaque samples were collected from caries-active subjects aged between 35 and 44 years, processed and cultured on mitis salivarius bacitracin agar...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29316616/revitalising-silver-nitrate-for-caries-management
#7
REVIEW
Sherry Shiqian Gao, Irene Shuping Zhao, Steve Duffin, Duangporn Duangthip, Edward Chin Man Lo, Chun Hung Chu
Silver nitrate has been adopted for medical use as a disinfectant for eye disease and burned wounds. In dentistry, it is an active ingredient of Howe's solution used to prevent and arrest dental caries. While medical use of silver nitrate as a disinfectant became subsidiary with the discovery of antibiotics, its use in caries treatment also diminished with the use of fluoride in caries prevention. Since then, fluoride agents, particularly sodium fluoride, have gained popularity in caries prevention. However, caries is an infection caused by cariogenic bacteria, which demineralise enamel and dentine...
January 6, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29315011/bioluminescence-analysis-of-antibacterial-photodynamic-therapy-using-methylene-blue-mediated-by-low-intensity-level-laser-against-cariogenic-biofilms
#8
Fernando Luis Florez Esteban, Morgana Regina Mendonça de Oliveira, Osmir Batista de Oliveira Júnior, Rochelle Denise Hiers, Sharukh Soli Khajotia, Hermes Pretel
OBJECTIVE: A non-destructive and real-time bioluminescence (BL) assay was used to determine the utility of antimicrobial photodynamic therapy (aPDT) treatments mediated by methylene blue (MB) and laser irradiation (LI) against intact biofilms that are capable of producing caries (cariogenic). The efficacy of antibacterial photodynamic treatments has been currently determined by using either viable colony counts (VCC) or metabolic assays (Alamar Blue) that were demonstrated to have critical limitations when used on microcolony-forming bacteria such as Streptococcus mutans...
January 9, 2018: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/29234121/in-silico-modelling-to-differentiate-the-contribution-of-sugar-frequency-versus-total-amount-in-driving-biofilm-dysbiosis-in-dental-caries
#9
David Head, Deirdre A Devine, P D Marsh
Dental caries is the most prevalent infection globally and a substantial economic burden in developed countries. Dietary sugars are the main risk factor, and drive increased proportions of acid-producing and acid-tolerating (aciduric) bacterial species within dental biofilms. Recent longitudinal studies have suggested that caries is most strongly correlated with total sugar intake, contrasting with the prevailing view that intake frequency is the primary determinant. To explore this possibility, we employed a computational model for supragingival plaque to systematically sample combinations of sugar frequency and total amount, allowing their independent contributions on the ratio of aciduric (i...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29228383/oral-fungal-bacterial-biofilm-models-in-vitro-a-review
#10
Marlene Chevalier, Stephane Ranque, Isabelle Prêcheur
Inclusion of fungi as commensals in oral biofilm is an important innovation in oral biology, and this work aimed to review the literature on the available biofilm and related disease in vitro models. Actually, thousands of bacterial and around one hundred of fungal phylotypes can colonize the oral cavity. Taxonomic profiling combined with functional expression analysis has revealed that Candida albicans, Streptococcus mutans and prominent periodontopathogens are not always present or numerically important in candidiasis, caries, or periodontitis lesions...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29209624/in-vitro-antibacterial-activity-of-pomegranate-juice-and-peel-extracts-on-cariogenic-bacteria
#11
Gianmaria Fabrizio Ferrazzano, Elisa Scioscia, Daniela Sateriale, Gabiria Pastore, Roberta Colicchio, Chiara Pagliuca, Tiziana Cantile, Brunella Alcidi, Marco Coda, Aniello Ingenito, Elena Scaglione, Annunziata Gaetana Cicatiello, Maria Grazia Volpe, Michele Di Stasio, Paola Salvatore, Caterina Pagliarulo
Aim: To evaluate the antimicrobial activity of hydroalcoholic extracts of pomegranate (Punica granatum L.) peel and juice, against the microorganisms considered the main etiologic agents of dental caries. Methods: The values of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined against Streptococcus mutans Clarke ATCC® 25175™ strain and Rothia dentocariosa clinical isolate. Results: Peel extracts inhibit effectively the growth and survival of S...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29207708/in-vitro-anti-cariogenic-plaque-effects-of-essential-oils-extracted-from-culinary-herbs
#12
Kornsit Wiwattanarattanabut, Suwan Choonharuangdej, Theerathavaj Srithavaj
Introduction: Cariogenic bacteria including mutans streptococci and lactobacilli are partly but significantly involved in dental caries development. An effective prevention strategy against dental caries is to decrease the accumulation of this microbiota either in planktonic or in biofilm form. Aim: To examine the antimicrobial and anti-plaque effects of some culinary herbs (spices), so the herbs are plausibly used as alternative and effective herbal plaque control supplements to promote good oral health...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29201598/use-of-probiotics-and-oral-health
#13
REVIEW
Robert P Allaker, Abish S Stephen
Purpose of Review: The purpose of this study is to critically assess recent studies concerning the use of probiotics to control periodontal diseases, dental caries and halitosis (oral malodour). Recent Findings: Clinical studies have shown that probiotics when allied to conventional periodontal treatment can ameliorate microbial dysbiosis and produce significant improvement in clinical indicators of disease. However, this effect is often not maintained by the host after the end of probiotic use...
2017: Current Oral Health Reports
https://www.readbyqxmd.com/read/29201054/antibacterial-effects-of-natural-herbal-extracts-on-streptococcus-mutans-can-they-be-potential-additives-in-dentifrices
#14
Spoorthi Banavar Ravi, Sudarshini Nirupad, Prashanthi Chippagiri, Rohit Pandurangappa
Background: Many plants or herbs exhibit potent antimicrobial activity against various microorganisms. They have no side effects and presumably act against and modulate the factors that are crucial for microbial survival or their activity. Streptococcus mutans is a pioneer bacteria implicated in dental caries. This study aims to evaluate the antimicrobial activity of garlic bulbs, pudina leaves, and mango and eucalyptus twig extracts on Streptococcus mutans by evaluating their zone of inhibition and determining their minimum inhibitory concentration (MIC)...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/29192351/the-implication-of-probiotics-in-the-prevention-of-dental-caries
#15
REVIEW
Tzu-Hsing Lin, Chih-Hui Lin, Tzu-Ming Pan
The current oral health crisis, whose causes are varied and complex, necessitates timely oral evaluation and early detection and treatment of oral health problems. Dramatic changes in eating habits and lifestyles are associated with the recent decline in oral health. Probiotics are "good" bacteria that support digestion and a healthy immune system and offer various health benefits to the host. Traditionally, probiotics have been used to improve gut health; the most common uses have historically been as a treatment or prevention of gastrointestinal infections and disease...
November 30, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29191562/genetic-and-lifestyle-dependent-dental-caries-defined-by-the-acidic-proline-rich-protein-genes-prh1-and-prh2
#16
Nicklas Strömberg, Anders Esberg, Nongfei Sheng, Lena Mårell, Anna Löfgren-Burström, Karin Danielsson, Carina Källestål
Dental caries is a chronic infectious disease that affects billions of people with large individual differences in activity. We investigated whether PRH1 and PRH2 polymorphisms in saliva acidic proline-rich protein (PRP) receptors for indigenous bacteria match and predict individual differences in the development of caries. PRH1 and PRH2 variation and adhesion of indigenous and cariogenic (Streptococcus mutans) model bacteria were measured in 452 12-year-old Swedish children along with traditional risk factors and related to caries at baseline and after 5-years...
November 22, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29190700/evaluation-of-the-difference-in-caries-experience-in-diabetic-and-non-diabetic-children-a-case-control-study
#17
Stefano Lai, Maria Grazia Cagetti, Fabio Cocco, Dina Cossellu, Gianfranco Meloni, Guglielmo Campus, Peter Lingström
AIM: To evaluate the caries prevalence and related variables in Type 1 diabetic and non-diabetic children and among the diabetic children according to their metabolic status. METHODS: Sixty-eight diabetic and 136 non-diabetic children, matching by gender and age (4-14 years) were enrolled. The diabetic children were divided: a) 20 children in good metabolic control (Hb1ac≤7.5) and b) 48 children in bad metabolic control (Hb1ac>7.5). Dietary and oral hygiene habits were investigated...
2017: PloS One
https://www.readbyqxmd.com/read/29178764/initial-microbial-colonization-of-enamel-in-children-with-different-levels-of-caries-activity-an-in-situ-study
#18
Susann Hertel, Alexandra Wolf, Sabine Basche, Gabriele Viergutz, Stefan Rupf, Matthias Hannig, Christian Hannig
PURPOSE: To investigate patterns of overnight in situ microbial colonization of enamel in children. METHODS: Overall, 29 children (aged 5-9 years) participated in the study. Nine were caries-free with no decayed, missing, or filled teeth (DMFT), 11 were caries-rehabilitated (DMFT ≥ 2, no active carious lesions), and nine were caries-active (DMFT ≥ 2, at least two carious lesions). Bovine enamel samples were fixed on individual upper jaw splints stored overnight in situ...
June 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29178733/the-role-of-adhesive-materials-and-oral-biofilm-in-the-failure-of-adhesive-resin-restorations
#19
REVIEW
Roberto Pinna, Paolo Usai, Enrica Filigheddu, Franklin Garcia-Godoy, Egle Milia
PURPOSE: To critically discuss adhesive materials and oral cariogenic biofilm in terms of their potential relevance to the failures of adhesive restorations in the oral environment. METHODS: The literature regarding adhesive restoration failures was reviewed with particular emphasis on the chemistry of adhesive resins, weakness in dentin bonding, water fluids, cariogenic oral biofilm and the relations that influence failures. Particular attention was paid to evidence derived from clinical studies...
October 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29145870/bacillus-anthracis-gamma-phage-lysis-among-soil-bacteria-an-update-on-test-specificity
#20
Cari B Kolton, Nicole L Podnecky, Sean V Shadomy, Jay E Gee, Alex R Hoffmaster
BACKGROUND: Bacillus anthracis, which causes anthrax in humans and animals, is enzootic in parts of the U.S. state of Texas where cases are typically reported in animals annually. The gamma phage lysis assay is a common diagnostic method for identification of B. anthracis and is based on the bacterium's susceptibility to lysis. This test has been shown to be 97% specific for B. anthracis, as a small number of strains of other Bacillus spp. are known to be susceptible. In this study, we evaluated the performance of a combination of B...
November 16, 2017: BMC Research Notes
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