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https://www.readbyqxmd.com/read/28407420/dental-biofilm-infections-an-update
#1
REVIEW
Tove Larsen, Nils-Erik Fiehn
Teeth are colonized by oral bacteria from saliva containing more than 700 different bacterial species. If removed regularly, the dental biofilm mainly comprises oral streptococci and is regarded as resident microflora. But if left undisturbed, a complex biofilm containing up to 100 bacterial species at a site will build up and may eventually cause development of disease. Depending on local ecological factors, the composition of the dental biofilm may vary considerably. With access to excess carbohydrates, the dental biofilm will be dominated by mainly gram-positive carbohydrate-fermenting bacteria causing demineralization of teeth, dental caries, which may further lead to inflammation and necrosis in the pulp and periapical region, i...
April 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28403796/riboswitches-as-potential-targets-for-the-development-of-anti-biofilm-drugs
#2
José A Reyes-Darias, Tino Krell
In nature, bacteria can exist as single motile cells or as sessile cellular community, known as microbial biofilms. Bacteria within biofilms are embedded in a self-produced extracellular matrix that makes them more resistant to antibiotic treatment and responses of the host immune system. Microbial biofilms are very important in medicine since they are associated with several human diseases such as dental caries, periodontitis, otitis media, infective endocarditis, infectious kidney stones, osteomyelitis or prostatitis...
April 7, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28377661/awareness-of-lebanese-pediatricians-regarding-children-s-oral-health
#3
Nahla Nassif, Balsam Noueiri, Riad Bacho, Kassem Kassak
Pediatricians have an important role in early identification and prevention of oral health, but they reported lack of knowledge in this field. AIM: the aim of the study is to evaluate the knowledge and attitudes of Lebanese pediatricians regarding children's oral health. MATERIALS AND METHODS: A cross sectional study including 100 Lebanese pediatricians was performed. They answered 21 questions. Three variables were taken into consideration: The number of years in practice, the place and type of practice...
January 2017: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28372291/current-challenges-in-human-tooth-revitalization
#4
Maxime Ducret, Hugo Fabre, Alexis Celle, Frédéric Mallein-Gerin, Emeline Perrier-Groult, Brigitte Alliot-Licht, Jean-Christophe Farges
Tooth vitality and health are related to the presence of a living connective tissue, the dental pulp (DP), in the center of the dental organ. The DP contains the tooth immune defence system that is activated against invading oral cariogenic bacteria during the caries process and the tissue repair/regeneration machinery involved following microorganisms' eradication. However, penetration of oral bacteria into the DP often leads to complete tissue destruction and colonization of the endodontic space by microorganisms...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28367914/plasma-treated-water-eliminates-streptococcus-mutans-in-infected-dentin-model
#5
Tatsuya Tasaki, Tomoko Ohshima, Emi Usui, Satoshi Ikawa, Katsuhisa Kitano, Nobuko Maeda, Yasuko Momoi
Non-mechanical procedures for removing caries-infected dentin are warranted in dentistry. We previously demonstrated the marked sterilization effect for direct irradiation of low-temperature plasma using dentin model infected with Streptococcus mutans. However it requires 180 s of intraoral plasma irradiation to eliminate bacteria. We alternatively investigated whether plasma-treated water (PTW), i.e., pure water exposed to plasma in an atmosphere, has a same bactericidal activity with the plasma irradiation...
March 29, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28358306/effect-of-the-biofilm-age-and-starvation-on-acid-tolerance-of-biofilm-formed-by-streptococcus-mutans-isolated-from-caries-active-and-caries-free-adults
#6
Shan Jiang, Shuai Chen, Chengfei Zhang, Xingfu Zhao, Xiaojing Huang, Zhiyu Cai
Streptococcus mutans (S. mutans) is considered a leading cause of dental caries. The capability of S. mutans to tolerate low pH is essential for its cariogenicity. Aciduricity of S. mutans is linked to its adaptation to environmental stress in oral cavity. This study aimed to investigate the effect of biofilm age and starvation condition on acid tolerance of biofilm formed by S. mutans clinical isolates. S. mutans clinical strains isolated from caries-active (SM593) and caries-free (SM18) adults and a reference strain (ATCC25175) were used for biofilm formation...
March 30, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28358034/in-silico-identification-of-potential-inhibitors-targeting-streptococcus-mutans-sortase-a
#7
Hao Luo, Dan-Feng Liang, Min-Yue Bao, Rong Sun, Yuan-Yuan Li, Jian-Zong Li, Xin Wang, Kai-Min Lu, Jin-Ku Bao
Dental caries is one of the most common chronic diseases and is caused by acid fermentation of bacteria adhered to the teeth. Streptococcus mutans (S. mutans) utilizes sortase A (SrtA) to anchor surface proteins to the cell wall and forms a biofilm to facilitate its adhesion to the tooth surface. Some plant natural products, especially several flavonoids, are effective inhibitors of SrtA. However, given the limited number of inhibitors and the development of drug resistance, the discovery of new inhibitors is urgent...
March 2017: International Journal of Oral Science
https://www.readbyqxmd.com/read/28336976/the-hall-technique-10-years-on-questions-and-answers
#8
N P T Innes, D J P Evans, C C Bonifacio, M Geneser, D Hesse, M Heimer, M Kanellis, V Machiulskiene, J Narbutaité, I C Olegário, A Owais, M P Araujo, D P Raggio, C Splieth, E van Amerongen, K Weber-Gasparoni, R M Santamaria
It is ten years since the first paper on the Hall Technique was published in the British Dental Journal and almost 20 years since the technique first came to notice. Dr Norna Hall a (now retired) general dental practitioner from the north of Scotland had, for many years, been managing carious primary molar teeth by cementing preformed metal crowns over them, with no local anaesthesia, tooth preparation or carious tissue removal. This first report, a retrospective analysis of Dr Hall's treatments, caused controversy...
March 24, 2017: British Dental Journal
https://www.readbyqxmd.com/read/28335264/antimicrobial-properties-of-biofunctionalized-silver-nanoparticles-on-clinical-isolates-of-streptococcus-mutans-and-its-serotypes
#9
Ángel Manuel Martínez-Robles, Juan Pablo Loyola-Rodríguez, Norma Verónica Zavala-Alonso, Rita Elizabeth Martinez-Martinez, Facundo Ruiz, René Homero Lara-Castro, Alejandro Donohué-Cornejo, Simón Yobanny Reyes-López, León Francisco Espinosa-Cristóbal
(1) Background: Streptococcus mutans (S. mutans) is the principal pathogen involved in the formation of dental caries. Other systemic diseases have also been associated with specific S. mutans serotypes (c, e, f, and k). Silver nanoparticles (SNP) have been demonstrated to have good antibacterial effects against S. mutans; therefore, limited studies have evaluated the antimicrobial activity of biofunctionalized SNP on S. mutans serotypes. The purpose of this work was to prepare and characterize coated SNP using two different organic components and to evaluate the antimicrobial activity of SNP in clinical isolates of S...
July 22, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28327128/the-investigation-of-helicobacter-pylori-in-the-dental-biofilm-and-saliva-samples-of-children-with-dyspeptic-complaints
#10
Damla Aksit Bıcak, Serap Akyuz, Binnur Kıratlı, Merve Usta, Nafiye Urganci, Burcin Alev, Aysen Yarat, Fikrettin Sahin
BACKGROUND: The oral cavity can be an extra-gastric reservoir for Helicobacter pylori (H.pylori). This can play a role in the pathogenesis of halitosis, glossitis, recurrent aphthous stomatitis, and dental caries. The present study was conducted to detect the presence of H.pylori within the dental biofilm and in saliva samples collected from children suffering from dyspepsia and children without any gastrointestinal complaints. Associations with gastric infection, halitosis, and some oral parameters were also evaluated...
March 21, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28326737/-research-progress-on-a-nanodrug-delivery-system-for-prevention-and-control-of-dental-caries-and-periodontal-diseases
#11
REVIEW
Jiang Yaling, Feng Mingye, Cheng Lei
Dental caries and periodontal diseases are common chronic infectious diseases that cause serious damage to oral health. Bacteria is the primary factor leading to such conditions. As a dental plaque control method, chemotherapeutic agents face serious challenges in dental care because of the specific physiological and anatomical characteristics of the oral cavity. Nanodrug delivery system is a series of new drug delivery systems at nanoscale, and it can target cells, promote sustainedrelease effects, and enhance biodegradation...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28326155/dental-plaque-microbial-profiles-of-children-from-khartoum-sudan-with-congenital-heart-defects
#12
Hiba Mohamed Ali, Ellen Berggreen, Daniel Nguyen, Raouf Wahab Ali, Thomas E Van Dyke, Hatice Hasturk, Manal Mustafa
Few studies have focused on the bacterial species associated with the deterioration of the dental and gingival health of children with congenital heart defects (CHD). The aims of this study were (1) to examine the dental plaque of children with CHD in order to quantify bacterial load and altered bacterial composition compared with children without CHD; and (2) to investigate the correlation between the level of caries and gingivitis and dental biofilm bacteria among those children. In this cross-sectional study, participants were children (3-12 years) recruited in Khartoum State, Sudan...
2017: Journal of Oral Microbiology
https://www.readbyqxmd.com/read/28317562/normal-oral-flora-and-the-oral-ecosystem
#13
REVIEW
Lakshman Samaranayake, Victor H Matsubara
The oral ecosystem comprises the oral flora, so-called oral microbiome, the different anatomic microniches of the oral cavity, and its bathing fluid, saliva. The oral microbiome comprises a group of organisms and includes bacteria, archaea, fungi, protozoa, and viruses. The oral microbiome exists suspended in saliva as planktonic phase organisms or attached to oral surfaces as a plaque biofilm. Homeostasis of the plaque biofilm and its symbiotic relationship with the host is critical for oral health. Disequilibrium or dysbiosis within the plaque biofilms is the initiating event that leads to major oral diseases, such as caries and periodontal disease...
April 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/28303827/characterisation-of-the-human-oral-microbiome-in-patients-with-coronary-artery-disease-using-next-generation-sequencing-of-16srrna-amplicons
#14
Thangam Menon, Sathya Narayanan Gopalakrishnan, Rayvathy Balasubramanian, Stalin Roy Justin
INTRODUCTION: Oral health is suspected to be linked to heart disease since species of bacteria that cause periodontitis and dental caries have been found in the atherosclerotic plaque in arteries in the heart. OBJECTIVES: The aim of this study was to characterize the oral microbiome in patients with coronary artery disease (CAD) and in a patient with dental caries (DC) without any clinical symptoms of CAD. METHODS: DNA was extracted from the oral swabs collected from the patients and sequencing was performed by next generation sequencing method using Illumina (MiSeq) platform...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28288542/the-role-of-the-saliva-antioxidant-barrier-to-reactive-oxygen-species-with-regard-to-caries-development
#15
A Jurczak, D Kościelniak, A Skalniak, M Papież, P Vyhouskaya, W Krzyściak
OBJECTIVES: The aim of this study was to evaluate the role of the antioxidant barrier in the saliva of children with caries, and its impact on the colonization of cariogenic bacteria. METHODS: This is a cross-sectional study of 81 children aged 1-5 years. Antioxidant levels and salivary bacterial profiles were measured. Patients were divided into two groups as follows: initial stage decay, termed non-cavitated (1-2 in International Caries Detection and Assessment System (ICDAS)), and extensive decay, termed cavitated lesions (5-6 in ICDAS)...
March 13, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/28277944/the-galleria-mellonella-larvae-as-an-in-vivo-model-for-evaluation-of-shigella-virulence
#16
Shoshana Barnoy, Hanan Gancz, Yuewei Zhu, Cary L Honnold, Daniel V Zurawski, Malabi M Venkatesan
Shigella spp. causing bacterial diarrhea and dysentery are human enteroinvasive bacterial pathogens that are orally transmitted through contaminated food and water and cause bacillary dysentery. Although natural Shigella infections are restricted to humans and primates, several smaller animal models are used to analyze individual steps in pathogenesis. No animal model fully duplicates the human response and sustaining the models requires expensive animals, costly maintenance of animal facilities, veterinary services and approved animal protocols...
February 13, 2017: Gut Microbes
https://www.readbyqxmd.com/read/28268111/characterization-of-growth-inhibition-of-oral-bacteria-by-sophorolipid-using-a-microplate-format-assay
#17
Daniel K Y Solaiman, Richard D Ashby, Joseph Uknalis
Sophorolipid (SL) is a class of glycolipid biosurfactant produced by yeast and has potent antimicrobial activity against many microorganisms. In this paper, a microplate-based method was developed to characterize the growth inhibition by SL on five representative species of caries-causing oral bacteria. Bacterial growth on microplate in the absence and presence of varying concentrations of SL was continuously monitored by recording the absorbance at 600nm of the cultures using a microplate reader. The results showed that SL completely inhibited the growth of the Lactobacilli at ≥1mg/ml and the Streptococci at much lower concentrations of ≥50μg/ml...
March 6, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28262381/pediatric-sepsis-secondary-to-an-occult-dental-abscess-a%C3%A2-case-report
#18
Peter Holmberg, Thomas Hellmich, James Homme
BACKGROUND: In general, hematogenous spread of bacteria in children is uncommon. Bacteremia, however, is a known complication of dental procedures and severe caries, but is infrequently associated with primary, asymptomatic, non-procedural-related, dentoalveolar infection. CASE REPORT: The patient is a 7-year-old previously healthy boy who presented to the Emergency Department (ED) with "fever, mottling, and shaking chills." In the ED, he appeared systemically ill with fever, mottling, delayed capillary refill, and rigors...
March 2, 2017: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28253872/midwestern-latino-caregivers-knowledge-attitudes-and-sense-making-of-the-oral-health-etiology-prevention-and-barriers-that-inhibit-their-children-s-oral-health-a-cbpr-approach
#19
Kimberly K Walker, E Angeles Martínez-Mier, Armando E Soto-Rojas, Richard D Jackson, Sarah M Stelzner, Lorena C Galvez, Gabriela J Smith, Miriam Acevedo, Laura Dandelet, Dulce Vega
BACKGROUND: Using community-based participatory research, the Health Protection Model was used to understand the cultural experiences, attitudes, knowledge and behaviors surrounding caries etiology, its prevention and barriers to accessing oral health care for children of Latino parents residing in Central Indiana. METHODS: A community reference group (CBPR) was established and bi-lingual community research associates were used to conduct focus groups comprised of Latino caregivers...
March 2, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28209019/efficacy-of-specific-plant-products-on-microorganisms-causing-dental-caries
#20
M Rajini Kanth, A Ravi Prakash, G Sreenath, Vikram Simha Reddy, S Huldah
INTRODUCTION: Dental caries and periodontal diseases are the most common oral diseases seen globally, both in developed and developing countries. Oral microorganisms that is gram positive and gram negative bacteria are known to be involved in causation of these diseases. Nowadays commercially available dentrifices and mouth rinses are known to contain ingredients that can alter the oral microbial flora and have undesirable side effects such as vomiting, diarrhoea, disarrangement of oral, intestinal flora and tooth staining...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
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