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https://www.readbyqxmd.com/read/28650782/effect-of-probiotic-yogurt-and-xylitol-containing-chewing-gums-on-salivary-s-mutans-count
#1
Elnaz Ghasemi, Romina Mazaheri, Arezoo Tahmourespour
BACKGROUND AND AIMS: In addition to improving gastrointestinal health and intestinal microflora, probiotic bacteria have been recently suggested to decrease cariogenic agents in the oral cavity. The aim of this study was to investigate the effects of probiotic yogurt and xylitol-containing chewing gums on reducing salivary Streptococcus mutans levels. STUDY DESIGN: This randomized clinical trial recruited 50 female students with over 10(5) colony forming units S...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28650087/educational-strategies-and-art-effect-on-salivary-characteristics-a-controlled-clinical-trial
#2
Camila Nobre de Freitas, Paula Midori Castelo, Karina Guedes de Sousa, Gabriela Caroline Alonso, Fernando Luiz Affonso Fonseca, Marlise Inês Klein, Taís de Souza Barbosa
OBJECTIVE: To evaluate educational strategies and ART-restoration impact on salivary physicochemical and microbiological characteristics. DESIGN: Two groups of 6-7-yr-old children were included: GART , with at least one decayed primary molar (n=36), submitted to 4 sessions of oral health educational strategy (OHES) and ART-restoration; GC , a paired caries-free group (n=36), submitted to 4 sessions of OHES. Three evaluations were carried out: baseline, one week after OHES and one month after OHES or ART, when biofilm and gingivitis frequencies, salivary flow, pH, buffer capacity, calcium and phosphorus concentrations were assessed...
June 26, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28649626/investigation-of-simulated-microgravity-effects-on-streptococcus-mutans-physiology-and-global-gene-expression
#3
Silvia S Orsini, April M Lewis, Kelly C Rice
Astronauts have been previously shown to exhibit decreased salivary lysozyme and increased dental calculus and gingival inflammation in response to space flight, host factors that could contribute to oral diseases such as caries and periodontitis. However, the specific physiological response of caries-causing bacteria such as Streptococcus mutans to space flight and/or ground-based simulated microgravity has not been extensively investigated. In this study, high aspect ratio vessel S. mutans simulated microgravity and normal gravity cultures were assessed for changes in metabolite and transcriptome profiles, H2O2 resistance, and competence in sucrose-containing biofilm media...
2017: NPJ Microgravity
https://www.readbyqxmd.com/read/28647333/effects-of-bedding-with-recycled-sand-on-lying-behaviors-udder-hygiene-and-preference-of-lactating-holstein-dairy-cows
#4
J A Kull, H D Ingle, R A Black, N L Eberhart, P D Krawczel
Effects of bedding with recycled sand and season on lying behaviors, hygiene, and preferences of late-lactation Holstein cows were studied. It was hypothesized that recycled sand will decrease lying time and increase hygiene scores due to increased moisture content and organic matter, and thus a preference for the control sand will be evident. Cows (n = 64) were divided into 4 groups (n = 8 per group) per season. In summer (August to September), cows were balanced by days in milk (268.1 ± 11.9 d) and parity (2...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28638559/comparative-evaluation-of-anti-microbial-effects-of-triple-antibiotic-paste-and-amox-and-its-derivatives-against-e-faecalis-an-in-vitro-study
#5
Manjeet Kaur, Shrikant Kendre, Parmod Gupta, Navneet Singh, Harsimran Sethi, Neha Gupta, Rushil Acharya
BACKGROUND: Enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections. Triple antibiotic paste has stood the test of time as a proven antibiotic combination against E. Faecalis. However, problems with this include staining of teeth and standardization of the preparation. Thus, the search for better alternatives and better preparation techniques is still on. AIM: To observe the potential of combinations [(Amoxicillin+ Metronidazole, Amoxicillin Clavulanic Acid + Metronidazole; Amoxicillin and Cloxacillin + Metronidazole)] over Triple Antibiotic Paste...
June 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28634492/effectiveness-of-different-preventive-programs-in-cariogram-parameters-of-young-adults-at-high-caries-risk
#6
Said Karabekiroğlu, Nimet Ünlü
OBJECTIVE: To evaluate the effectiveness of different preventive programs in young adults at high caries risk using Cariogram software. METHODS: Sixty-six young adults with high caries risk were evaluated. Dental caries risk for all subjects was determined according to WHO criteria. Subjects were divided into three different preventive groups (control: OH, fluoride varnish: FV, and chlorhexidine varnish: CV). They were followed for 12 weeks (baseline: T0, 1 week: T1, 4 weeks: T2, and 12 weeks: T3)...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/28621288/photodvnamic-therapy-in-endodontics
#7
Zahed Mohammadi, Hamid Jafarzadeh, Sousan Shalavi, Jun-Ichiro Kinoshita
Commonly used irrigants do not always eradicate the entire microbial flora in infected root canals. Therefore, several other strategies, such as photodynamic therapy (PDT) have been developed. Photoactivated disinfection is based on the interaction of a photosensitive antibacterial agent and a light source. It uses a nontoxic dye named photosensitizer (PS) and low-intensity visible light. In oxygen presentation, these combine to produce some cytotoxic species. The PS molecules attach to bacteria membrane. Irradiation with a specific wavelength of the light may lead to the production of singlet oxygen, resulting in rupture of the microbial cell wall...
June 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28603127/antibacterial-activities-of-diospyros-blancoi-phoenix-dactylifera-and-morus-nigra-against-dental-caries-causing-pathogens-an-in-vitro-study
#8
Lubna Tahir, Ayesha Aslam, Safia Ahmed
The study was undertaken to determine the in-vitro antibacterial potential of Diospyrosblancoi, Phoenix dactylifera and Morusnigra leaf extracts in hexane, chloroform, methanol, ethyl-acetate and aqueous extracts against dental caries causing bacteria. Disc diffusion assay was used to determine the antibacterial efficacy; the extracts were further separated using Thin Layer Chromatography and the anti-biofilm activity of the extracts was also determined. The preliminary phytochemical screening of the extracts revealed the presences of flavonoids, saponins, alkaloids and tannins because of which the extracts showed strong antibacterial activity against the selected pathogens...
January 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28589101/recombineering-in-streptococcus-mutans-using-direct-repeat-mediated-cloning-independent-markerless-mutagenesis-dr-cimm
#9
Shan Zhang, Zhengzhong Zou, Jens Kreth, Justin Merritt
Studies of the dental caries pathogen Streptococcus mutans have benefitted tremendously from its sophisticated genetic system. As part of our own efforts to further improve upon the S. mutans genetic toolbox, we previously reported the development of the first cloning-independent markerless mutagenesis (CIMM) system for S. mutans and illustrated how this approach could be adapted for use in many other organisms. The CIMM approach only requires overlap extension PCR (OE-PCR) protocols to assemble counterselectable allelic replacement mutagenesis constructs, and thus greatly increased the speed and efficiency with which markerless mutations could be introduced into S...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28587978/a-new-arginine-based-dental-adhesive-system-formulation-mechanical-and-anti-caries-properties
#10
Saulo Geraldeli, Eveline F Soares, Andres J Alvarez, Tanaz Farivar, Robert C Shields, Mario A C Sinhoreti, Marcelle M Nascimento
Secondary caries at the margins of composite restorations has been attributed to adhesive failure and consequent accumulation of cariogenic biofilms. OBJECTIVES: To develop and evaluate an etch-and-rinse adhesive system containing arginine for sustainable release and recharge without affecting its mechanical properties. Arginine metabolism by oral bacteria generates ammonia, which neutralizes glycolytic acids and creates a neutral environmental pH that is less favorable to the growth of caries pathogens, thus reducing the caries risk at the tooth-composite interface...
June 3, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28586050/inhibitory-capacity-of-rhus-coriaria-l-extract-and-its-major-component-methyl-gallate-on-streptococcus-mutans-biofilm-formation-by-optical-profilometry-potential-applications-for-oral-health
#11
Tomas Kacergius, Saleh Abu-Lafi, Agne Kirkliauskiene, Vika Gabe, Azmi Adawi, Mahmoud Rayan, Mutaz Qutob, Rimantas Stukas, Algirdas Utkus, Mouhammad Zeidan, Anwar Rayan
Streptococcus mutans (S. mutans) bacterium is the most well recognized pathogen involved in pathogenesis of dental caries. Its virulence arises from its ability to produce a biofilm and acidogenicity, causing tooth decay. Discovery of natural products capable to inhibit biofilm formation is of high importance for developing health care products. To the best of our knowledge, in all previous scientific reports, a colorimetric assay was applied to test the effect of sumac and methyl gallate (MG) on S. mutans adherence...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28571698/a-novel-antimicrobial-peptide-against-dental-caries-associated-bacteria
#12
Long Chen, Lili Jia, Qiang Zhang, Xirui Zhou, Zhuqing Liu, Bingjie Li, Zhentai Zhu, Fenwei Wang, Changyuan Yu, Qian Zhang, Feng Chen, Shi-Zhong Luo
Dental caries, a highly prevalent oral disease, is primarily caused by pathogenic bacteria infection, and most of them are anaerobic. Herein, we investigated the activity of a designed antimicrobial peptide ZXR-2, and found it showed broad-spectrum activity against a variety of Gram-positive and Gram-negative oral bacteria, particularly the caries-related taxa Streptococcus mutans. Time-course killing assays indicated that ZXR-2 killed most bacterial cells within 5 min at 4 × MIC. The mechanism of ZXR-2 involved disruption of cell membranes, as observed by scanning electron microscopy...
May 29, 2017: Anaerobe
https://www.readbyqxmd.com/read/28571487/targeting-of-streptococcus-mutans-biofilms-by-a-novel-small-molecule-prevents-dental-caries-and-preserves-the-oral-microbiome
#13
S S Garcia, M S Blackledge, S Michalek, L Su, T Ptacek, P Eipers, C Morrow, E J Lefkowitz, C Melander, H Wu
Dental caries is a costly and prevalent disease characterized by the demineralization of the tooth's enamel. Disease outcome is influenced by host factors, dietary intake, cariogenic bacteria, and other microbes. The cariogenic bacterial species Streptococcus mutans metabolizes sucrose to initiate biofilm formation on the tooth surface and consequently produces lactic acid to degrade the tooth's enamel. Persistence of S. mutans biofilms in the oral cavity can lead to tooth decay. To date, no anticaries therapies that specifically target S...
July 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28567481/analysis-of-sucrose-induced-small-rnas-in-streptococcus-mutans-in-the-presence-of-different-sucrose-concentrations
#14
Shan Shan Liu, Wen Hui Zhu, Qing Hui Zhi, Jia Liu, Yan Wang, Huan Cai Lin
Streptococcus mutans (S. mutans) is the major pathogen contributing to dental caries. Sucrose is an important carbohydrate source for S. mutans and is crucial for dental caries. Small RNAs (sRNAs) are key post-transcriptional regulators of stress adaptation and virulence in bacteria. Here, for the first time, we created three replicate RNA libraries exposed to either 1 or 5% sucrose. The expression levels of sRNAs and target genes (gtfB, gtfC, and spaP) related to virulence were assessed. In addition, some phenotypic traits were evaluated...
May 31, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28555200/chlorhexidine-mouthwash-as-an-anticaries-agent-%C3%A2-a-systematic-review
#15
Ana Sofia Estima Cunha Coelho, Anabela Baptista Pereira Paula, Teresa Maria Palmeirão Carrilho, Mário Jorge Rebolho Fernandes da Silva, Maria Filomena Rabaça Roque Botelho, Eunice Virgínia Valdez Faria Carrilho
OBJECTIVE: To evaluate the effectiveness of chlorhexidine mouthwashes on the reduction of cariogenic bacteria on patients with moderate to high risk for dental caries. DATA SOURCES: A systematic review of the literature was performed using Cochrane Library, Embase, PubMed, Scopus and Web of Science. The search was limited to articles in English, Spanish, and Portuguese, published until January 2017. The research question was formulated following the PICO strategy...
2017: Quintessence International
https://www.readbyqxmd.com/read/28554609/biofilm-induced-changes-to-the-composite-surface
#16
Ivana Nedeljkovic, Jan De Munck, Andreea-Alexandra Ungureanu, Vera Slomka, Carmen Bartic, Anja Vananroye, Christian Clasen, Wim Teughels, Bart Van Meerbeek, Kirsten L Van Landuyt
OBJECTIVES: Composites may undergo biodegradation in the oral cavity. The objective was to investigate the effect of single- and multi-species biofilms on the surface roughness and topography of two composites. METHODS: Disk-shaped specimens of a paste-like, Bis-GMA-free (Gradia Direct Anterior, GC), and a flowable, Bis-GMA-based composite (Tetric EvoFlow, Ivoclar-Vivadent) were prepared. After ethylene-oxide sterilization (38°C), specimens (n=3) were incubated with Streptococcus mutans or mixed bacterial culture (Streptococcus mutans, Streptococcus sanguinis, Actinomyces naeslundii and Fusobacterium nucleatum)...
May 26, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28554028/the-effects-of-low-level-ionizing-radiation-and-copper-exposure-on-the-incidence-of-antibiotic-resistance-in-lentic-biofilm-bacteria
#17
J Vaun McArthur, Christian A Dicks, A Lawrence Bryan, R Cary Tuckfield
Environmental reservoirs of antibiotic resistant bacteria are poorly understood. Understanding how the environment selects for resistance traits in the absence of antibiotics is critical in developing strategies to mitigate this growing menace. Indirect or co-selection of resistance by environmental pollution has been shown to increase antibiotic resistance. However no attention has been given to the effects of low-level ionizing radiation or the interactions between radiation and heavy metals on the maintenance or selection for antibiotic resistance (AR) traits...
May 26, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28549260/identification-of-oral-cavity-biofilm-forming-bacteria-and-determination-of-their-growth-inhibition-by-acacia-arabica-tamarix-aphylla-l-and-melia-azedarach-l-medicinal-plants
#18
Muhammad Khalid, Danial Hassani, Muhammad Bilal, Zahid Ali Butt, Muhammad Hamayun, Ayaz Ahmad, Danfeng Huang, Anwar Hussain
OBJECTIVE: Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains. METHODS: Pathogenic bacteria from dental biofilms were isolated, cultured, identified by phylogenetic analysis using PCR-based 16S ribosomal RNA (or 16S rRNA) nucleotide sequences and were analyzed for their biofilm forming capability...
May 18, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28542863/mechanisms-of-silver-diamine-fluoride-on-arresting-caries-a-literature-review
#19
REVIEW
Irene Shuping Zhao, Sherry Shiqian Gao, Noriko Hiraishi, Michael Francis Burrow, Duangporn Duangthip, May Lei Mei, Edward Chin-Man Lo, Chun-Hung Chu
OBJECTIVE: To review the evidence regarding the mechanisms of silver diamine fluoride (SDF) for arresting caries. METHODS: A literature search was conducted using the keywords silver diamine fluoride, and its alternative names, in seven databases: PubMed, Embase and Scopus (English); China National Knowledge Infrastructure (Chinese); Bilioteca Virtual em Saude (Portuguese); Biblioteca Virtual en Salud Espana (Spanish); and Ichushi-Web (Japanese). The titles and abstracts were screened...
May 21, 2017: International Dental Journal
https://www.readbyqxmd.com/read/28536781/oral-health-status-in-adult-patients-with-newly-diagnosed-acute-leukemia
#20
Rilana Busjan, Justin Hasenkamp, Gerhard Schmalz, Rainer Haak, Lorenz Trümper, Dirk Ziebolz
BACKGROUND: The aim of this cross-sectional study was to evaluate the oral health of adult patients with newly diagnosed acute leukemia. METHODS: Patients with initially diagnosed acute myeloid (AML) or lymphocytic (ALL) leukemia and a matched healthy control (HC) group were included. The oral investigation comprised inspection of the oral mucosa; the decayed (D), missing (M), and filled (F) teeth (DMF-T) index; and a detailed periodontal status. Subgingival biofilm samples were analyzed (polymerase chain reaction) for the presence of selected potentially periodontal pathogenic bacteria...
May 23, 2017: Clinical Oral Investigations
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