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https://www.readbyqxmd.com/read/28823045/killing-activity-of-lfchimera-on-periodontopathic-bacteria-and-multispecies-oral-biofilm-formation-in-vitro
#1
Sopita Ruangcharoen, Waraporn Suwannarong, Marie Rossini Carmela T Lachica, Jan G M Bolscher, Kamran Nazmi, Watcharee Khunkitti, Suwimol Taweechaisupapong
Lactoferrin chimera (LFchimera), a heterodimeric peptide containing lactoferrampin (LFampin265-284) and a part of lactoferricin (LFcin17-30), possesses a broad spectrum of antimicrobial activity. However, there is no report on the inhibitory effects of LFchimera against multispecies oral biofilms. This study aimed to determine the effects of LFchimera in comparison to chlorhexidine digluconate (CHX) and minocycline hydrochloride (MH), on in vitro multispecies biofilms derived from subgingival plaque of periodontitis patients harboring Aggregatibacter actinomycetemcomitans...
August 19, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28821551/coordinated-regulation-of-the-eii-man-and-frurki-operons-of-streptococcus-mutans-by-global-and-fructose-specific-pathways
#2
Lin Zeng, Brinta Chakraborty, Tanaz Farivar, Robert A Burne
The glucose/mannose-PTS permease EII(Man) encoded by manLMN in the dental caries pathogen Streptococcus mutans has a dominant influence on sugar-specific, CcpA-independent catabolite repression (CR). Mutations in manL affect energy metabolism and virulence-associated traits, including biofilm formation, acid tolerance and competence. Using promoter:reporter fusions, expression of the manLMN and the fruRKI operons, encoding a transcriptional regulator, a fructose-1-P kinase and a fructose-PTS permease EII(Fru), respectively, was monitored in response to carbohydrate source and in mutants lacking CcpA, FruR, and components of EII(Man) Expression of genes for EII(Man) and EII(Fru) was directly regulated by CcpA and CR, as evinced by in vivo and in vitro methods...
August 18, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28821458/dipeptidyl-peptidase-iv-and-quorum-sensing-signaling-in-biofilm-related-virulence-of-prevotella-aurantiaca
#3
Dareen Fteita, Ahmed Ali Musrati, Eija Könönen, Xiaochu Ma, Mervi Gürsoy, Markus Peurla, Eva Söderling, Herman O Sintim, Ulvi Kahraman Gürsoy
Biofilm formation and dipeptidyl peptidase IV (DPPIV) enzyme activity contribute to the virulence of oral bacteria, and these virulence factors are partly regulated by quorum sensing signaling system. We recently demonstrated that estradiol regulates growth properties and DPPIV activity of Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens. Here, we examined the DPPIV dependency of biofilm formation of Prevotella aurantiaca. Three strains (two clinical strains AHN 37505 and 37552 and the type strain CCUG 57723) were incubated in three estradiol concentrations (30, 90, and 120 nmol/L)...
August 15, 2017: Anaerobe
https://www.readbyqxmd.com/read/28818264/the-potential-management-of-oral-candidiasis-using-anti-biofilm-therapies
#4
Warren Chanda, Thomson P Joseph, Wendong Wang, Arshad A Padhiar, Mintao Zhong
Candida albicans is a minor component of the oral microbiota and an opportunistic pathogen that takes advantage of the immunocompromised host and causes oral mucositis and oral candidiasis. This organism is able to undergo phenotypic modification from a yeast to hyphae growth phase, one of the key arsenals for immune cell evasion, tissue invasion and biofilm formation. The latter property coupled with overgrowth and immune compromising factors such as HIV/AIDS, cancer treatments, organ transplantation, diabetes, corticosteroid use, dentures, and broad-spectrum antibiotic use have modified the fungus from a normal component of the microflora to a foe of an oral cavity and resulting in reduced sensitivity towards commonly utilised antifungal agents...
September 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28809644/-endoprosthesis-infections-guidelines-for-antibiotic-therapy-common-guidelines-of-the-czech-society-for-orthopaedics-and-traumatology-and-the-society-for-infectious-diseases-of-the-czech-medical-association-of-j-e-purkyn%C3%A4
#5
D Musil, M Balejová, M Horníková, A Chrdle, N Mallátová, O Nyč, V Chmelík, J Gallo, D Jahoda, J Stehlík
PURPOSE OF THE STUDY This study aims to articulate regional guidelines for curative and suppressive antibiotic therapy of total joint replacement infections. MATERIAL AND METHODS When developing the standard, used as source materials were the published foreign guidelines for antibiotic therapy of prosthetic joint infections, the analysis of resistance of bacterial strains conducted in the Hospital in České Budějovice, a.s. and the assessment of strain resistance for the Czech Republic published by the European Antimicrobial Resistance Surveillance Network (EARS-Net)...
2017: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/28807017/the-urinary-microbiota-of-men-and-women-and-its-changes-in-women-during-bacterial-vaginosis-and-antibiotic-treatment
#6
Cornelia Gottschick, Zhi-Luo Deng, Marius Vital, Clarissa Masur, Christoph Abels, Dietmar H Pieper, Irene Wagner-Döbler
BACKGROUND: The urinary microbiota is similarly complex as the vaginal and penile microbiota, yet its role as a reservoir for pathogens and for recurrent polymicrobial biofilm diseases like bacterial vaginosis (BV) is not clear. RESULTS: Here, we analysed the urinary microbiota of healthy men and women and compared it with that of women during BV and after antibiotic treatment using next-generation sequencing of the 16S rRNA gene V1-V2 regions. Eight different community types, so called urotypes (UT), were identified in healthy humans, all of which were shared between men and women, except UT 7, dominated in relative abundance by Lactobacillus crispatus, which was found in healthy women only...
August 14, 2017: Microbiome
https://www.readbyqxmd.com/read/28805207/periodontal-diseases
#7
REVIEW
Denis F Kinane, Panagiota G Stathopoulou, Panos N Papapanou
Periodontal diseases comprise a wide range of inflammatory conditions that affect the supporting structures of the teeth (the gingiva, bone and periodontal ligament), which could lead to tooth loss and contribute to systemic inflammation. Chronic periodontitis predominantly affects adults, but aggressive periodontitis may occasionally occur in children. Periodontal disease initiation and propagation is through a dysbiosis of the commensal oral microbiota (dental plaque), which then interacts with the immune defences of the host, leading to inflammation and disease...
June 22, 2017: Nature Reviews. Disease Primers
https://www.readbyqxmd.com/read/28797882/repurposing-antipsychotic-drugs-into-antifungal-agents-synergistic-combinations-of-azoles-and-bromperidol-derivatives-in-the-treatment-of-various-fungal-infections
#8
Selina Y L Holbrook, Atefeh Garzan, Emily K Dennis, Sanjib K Shrestha, Sylvie Garneau-Tsodikova
As the number of hospitalized and immunocompromised patients continues to rise, invasive fungal infections, such as invasive candidiasis and aspergillosis, threaten the life of millions of patients every year. The azole antifungals are currently the most prescribed drugs clinically that display broad-spectrum antifungal activity and excellent oral bioavailability. Yet, the azole antifungals have their own limitations and are unable to meet the challenges associated with increasing fungal infections and the accompanied development of resistance against azoles...
July 19, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28797211/disulfide-bonds-a-key-modification-in-bacterial-extracytoplasmic-proteins
#9
S F Lee, L Davey
Disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. Almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. Accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. Recent advances clearly show that oral bacteria also have the ability to from disulfide bonds, and this ability has an effect on a range of dental plaque-related phenotypes...
August 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28793083/oral-health-condition-and-reasons-for-tooth-extraction-among-an-adult-population-20-64-years-old
#10
Manoelito Ferreira Silva-Junior, Anne Caroline Correia de Sousa, Marília Jesus Batista, Maria da Luz Rosário de Sousa
The study characterized the oral health condition and main self-reported reasons for tooth extraction in an adult population. The cross-sectional study examined 248 adults aged 20-64 years, representative of the population of Piracicaba, São Paulo, Brazil. The oral examination conducted in households used the DMFT and CPI indexes, use and necessity of prosthodontics according to the WHO criteria and the presence of visible biofilm. Demographic and socioeconomic data were collected along with reasons for tooth extraction through a questionnaire...
August 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28792381/effects-of-tea-extracts-on-the-colonization-behaviour-of-candida-species-attachment-inhibition-and-biofilm-enhancement
#11
Yi Wang, H M H N Bandara, Deirdre Mikkelsen, Lakshman P Samaranayake
PURPOSE: We assessed the effects of four different types of tea extracts (green, oolong, black and pu-erh tea) on cellular surface properties (hydrophobicity and auto-aggregation) and the colonization attributes (attachment and biofilm formation) of four strains of Candida albicans and three strains of Candida krusei. METHODOLOGY: The cellular surface properties were determined using spectrophotometry. The colonization activities were quantified using colorimetric viability assays and visualized using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM)...
August 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28792372/treatment-failure-of-bacterial-vaginosis-is-not-associated-with-higher-loads-of-atopobium-vaginae-and-gardnerella-vaginalis
#12
Carolina Sanitá Tafner Ferreira, Gilbert Gerard Donders, Cristina Maria Garcia de Lima Parada, Andrea da Rocha Tristão, Thaiz Fernandes, Márcia Guimarães da Silva, Camila Marconi
PURPOSE: Cervicovaginal Atopobium vaginae and Gardnerella vaginalis are strongly associated with bacterial vaginosis (BV) and are the main components of vaginal biofilms. The low efficacy of BV treatment with metronidazole may be due to the presence of such biofilms. Thus, the aim of this study was to compare the pretreatment cervicovaginal loads of A. vaginae and G. vaginalis for women who restored normal flora and those who persisted with BV after a full course of oral metronidazole...
August 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28792235/antimicrobial-efficacy-of-irradiation-with-visible-light-on-oral-bacteria-in-vitro-a-systematic-review
#13
Andreas Pummer, Helge Knüttel, Karl-Anton Hiller, Wolfgang Buchalla, Fabian Cieplik, Tim Maisch
AIM: Resistances to antibiotics employed for treatment of infectious diseases have increased to alarming numbers making it more and more difficult to treat diseases caused by microorganisms resistant to common antibiotics. Consequently, novel methods for successful inactivation of pathogens are required. In this instance, one alternative could be application of light for treatment of topical infections. Antimicrobial properties of UV light are well documented, but due to its DNA-damaging properties use for medical purposes is limited...
August 9, 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/28790818/peptide-modified-nanoparticles-inhibit-formation-of-porphyromonas-gingivalis-biofilms-with-streptococcus-gordonii
#14
Paridhi Kalia, Ankita Jain, Ranjith Radha Krishnan, Donald R Demuth, Jill M Steinbach-Rankins
PURPOSE: The interaction of Porphyromonas gingivalis with commensal streptococci promotes P. gingivalis colonization of the oral cavity. We previously showed that a synthetic peptide (BAR) derived from Streptococcus gordonii potently inhibited the formation of P. gingivalis/S. gordonii biofilms (IC50 =1.3 µM) and reduced P. gingivalis virulence in a mouse model of periodontitis. Thus, BAR represents a novel therapeutic to control periodontitis by limiting P. gingivalis colonization of the oral cavity...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28782094/comparative-sem-analysis-of-nine-f22-aligner-cleaning-strategies
#15
Luca Lombardo, Marco Martini, Francesca Cervinara, Giorgio Alfredo Spedicato, Teresa Oliverio, Giuseppe Siciliani
BACKGROUND: The orthodontics industry has paid great attention to the aesthetics of orthodontic appliances, seeking to make them as invisible as possible. There are several advantages to clear aligner systems, including aesthetics, comfort, chairside time reduction, and the fact that they can be removed for meals and oral hygiene procedures. METHODS: Five patients were each given a series of F22 aligners, each to be worn for 14 days and nights, with the exception of meal and brushing times...
December 2017: Progress in Orthodontics
https://www.readbyqxmd.com/read/28779891/silver-nanoparticles-in-dentistry
#16
REVIEW
Victor T Noronha, Amauri J Paula, Gabriela Durán, Andre Galembeck, Karina Cogo-Müller, Michelle Franz-Montan, Nelson Durán
OBJECTIVE: Silver nanoparticles (AgNPs) have been extensively studied for their antimicrobial properties, which provide an extensive applicability in dentistry. Because of this increasing interest in AgNPs, the objective of this paper was to review their use in nanocomposites; implant coatings; pre-formulation with antimicrobial activity against cariogenic pathogens, periodontal biofilm, fungal pathogens and endodontic bacteria; and other applications such as treatment of oral cancer and local anesthesia...
August 2, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28775282/ecological-effect-of-arginine-on-oral-microbiota
#17
Xin Zheng, Jinzhi He, Lin Wang, Shuangshuang Zhou, Xian Peng, Shi Huang, Liwei Zheng, Lei Cheng, Yuqing Hao, Jiyao Li, Jian Xu, Xin Xu, Xuedong Zhou
Dental caries is closely associated with the microbial dybiosis between acidogenic/aciduric pathogens and alkali-generating commensal bacteria colonized in the oral cavity. Our recent studies have shown that arginine may represent a promising anti-caries agent by modulating microbial composition in an in vitro consortium. However, the effect of arginine on the oral microbiota has yet to be comprehensively delineated in either clinical cohort or in vitro biofilm models that better represent the microbial diversity of oral cavity...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28774007/do-dental-resin-composites-accumulate-more-oral-biofilms-and-plaque-than-amalgam-and-glass-ionomer-materials
#18
Ning Zhang, Mary A S Melo, Michael D Weir, Mark A Reynolds, Yuxing Bai, Hockin H K Xu
A long-time drawback of dental composites is that they accumulate more biofilms and plaques than amalgam and glass ionomer restorative materials. It would be highly desirable to develop a new composite with reduced biofilm growth, while avoiding the non-esthetics of amalgam and low strength of glass ionomer. The objectives of this study were to: (1) develop a protein-repellent composite with reduced biofilms matching amalgam and glass ionomer for the first time; and (2) investigate their protein adsorption, biofilms, and mechanical properties...
November 1, 2016: Materials
https://www.readbyqxmd.com/read/28772863/current-insights-into-the-modulation-of-oral-bacterial-degradation-of-dental-polymeric-restorative-materials
#19
REVIEW
Ning Zhang, Yansong Ma, Michael D Weir, Hockin H K Xu, Yuxing Bai, Mary Anne S Melo
Dental polymeric composites have become the first choice for cavity restorations due to their esthetics and capacity to be bonded to the tooth. However, the oral cavity is considered to be harsh environment for a polymeric material. Oral biofilms can degrade the polymeric components, thus compromising the marginal integrity and leading to the recurrence of caries. Recurrent caries around restorations has been reported as the main reason for restoration failure. The degradation of materials greatly compromises the clinical longevity...
May 6, 2017: Materials
https://www.readbyqxmd.com/read/28772615/streptococcus-sanguis-biofilm-architecture-and-its-influence-on-titanium-corrosion-in-enriched-artificial-saliva
#20
Lei Li, Shunling Li, Qing Qu, Limei Zuo, Yue He, Baolin Zhu, Cong Li
Bacteria biofilm formation on metals is well-known, while biofilm architecture varies under different conditions. To date, few studies have determined the possible contribution to corrosion of titanium made by biofilm architecture. We investigated the interaction between the oral Streptococcus sanguis biofilm architecture and its influence on titanium corrosion in enriched artificial saliva using electrochemical methods and microscopic study. Patchy biofilms were observed on titanium surface after being immersed in solution containing S...
March 3, 2017: Materials
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