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https://www.readbyqxmd.com/read/29050024/biofilm-control-and-oral-hygiene-practices
#1
Marisa Maltz, Luana Severo Alves, Julio Eduardo do Amaral Zenkner
As the thick biofilm in the presence of fermentable carbohydrates is the main etiological factor of dental caries, the frequent and systematic removal of this colony by means of an effective biofilm control should result in the prevention of caries lesions or in the arrest of the local carious process. However, the role of biofilm control in the management of dental caries has been questioned. This chapter will discuss the biofilm control and oral hygiene practices on root surfaces. Laboratory and clinical studies describing the effect of biofilm control and oral hygiene practices on the arrestment of root carious lesions are described...
2017: Monographs in Oral Science
https://www.readbyqxmd.com/read/29050017/specificities-of-caries-on-root-surface
#2
Nailê Damé-Teixeira, Clarissa Cavalcanti Fatturi Parolo, Marisa Maltz
Variations in organic and inorganic composition and morphology may determine different susceptibilities of root surfaces to caries. Subsequent to gingival recession, root surfaces become exposed and those areas where Sharpey's fibers system was once inserted into the cementum are converted into canals for microbial penetration. In the presence of a cariogenic root biofilm, the fermentable carbohydrate from diet is converted into organic acid, and the root caries lesion is initiated in the exposed root site...
2017: Monographs in Oral Science
https://www.readbyqxmd.com/read/29046620/the-dental-calculus-metabolome-in-modern-and-historic-samples
#3
Irina M Velsko, Katherine A Overmyer, Camilla Speller, Lauren Klaus, Matthew J Collins, Louise Loe, Laurent A F Frantz, Krithivasan Sankaranarayanan, Cecil M Lewis, Juan Bautista Rodriguez Martinez, Eros Chaves, Joshua J Coon, Greger Larson, Christina Warinner
INTRODUCTION: Dental calculus is a mineralized microbial dental plaque biofilm that forms throughout life by precipitation of salivary calcium salts. Successive cycles of dental plaque growth and calcification make it an unusually well-preserved, long-term record of host-microbial interaction in the archaeological record. Recent studies have confirmed the survival of authentic ancient DNA and proteins within historic and prehistoric dental calculus, making it a promising substrate for investigating oral microbiome evolution via direct measurement and comparison of modern and ancient specimens...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/29036235/-medicaments-and-oral-healthcare-5-adverse-effects-of-medications-and-over-the-counter-drugs-on-teeth
#4
C de Baat, P G M A Zweers, C van Loveren, A Vissink
Intrinsic tooth discoloration may occur as an adverse effect of fluoride and tetracyclines. Extrinsic tooth discoloration may occur as superficial staining or as discoloration of the superficial pellicle and/or biofilm due to chlorhexidine, liquid iron salts, essential oils, some antibiotics and stannous fluoride. Inhibition of orthodontic tooth movement has been reported due to the use of prostaglandin synthetase inhibitors. If medications or over-the-counter drugs induce hyposalivation or contain much sucrose, caries may develop...
October 2017: Nederlands Tijdschrift Voor Tandheelkunde
https://www.readbyqxmd.com/read/29034970/oral-health-of-older-residents-in-care-and-community-dwellers-nursing-implications
#5
J S Choi, Y J Yi, L R Donnelly
BACKGROUND: Poor oral health is associated with a reduced quality of life and serious illnesses. Consequently, nurses need to be more aware of oral health to improve the general health of residents in care. AIM: To evaluate and compare oral health behaviours and levels of salivary haemoglobin and dental biofilm acidogenicity, which can be used to evaluate oral disease activity, between residents and community dwellers in South Korea. METHODS: This cross-sectional study included 133 participants: 64 residents and 69 community dwellers...
October 16, 2017: International Nursing Review
https://www.readbyqxmd.com/read/29031238/the-effect-of-supragingival-biofilm-re-development-on-the-subgingival-microbiota-in-chronic-periodontitis
#6
Fátima Aparecida Rocha Resende Hartenbach, Carina Maciel Silva-Boghossian, Ana Paula Vieira Colombo
OBJECTIVE: In this study, we hypothesized that in the absence of oral hygiene, re-growth of the climax microbial communities of supra and subgingival biofilm happens in a faster and more intense fashion in individuals with chronic periodontitis (CP) compared to periodontally healthy controls (PH). DESIGN: Thirty patients (PH=15 and CP=15) received professional supragingival prophylaxis, and were asked to refrain from oral hygiene for 7days. Supra and subgingival biofilm samples and GCF were collected from randomly selected quadrants at baseline (before prophylaxis), immediately after prophylaxis, 2h, 6h, 24h, and 7days after prophylaxis...
October 10, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29031236/inhibitory-effect-of-probiotic-lactobacilli-supernatants-on-single-and-mixed-non-albicans-candida-species-biofilm
#7
Yulong Tan, Matthias Leonhard, Doris Moser, Su Ma, Berit Schneider-Stickler
OBJECTIVES: Oral candidiasis is one of the most common human fungal infections. While most cases of the Candida species isolated from the oral cavity are Candida albicans, a large number of candidiasis is attributed to non-albicans Candida species. In this study, we aim to evaluate the in vitro inhibition of supernatants of Lactobacillus gasseri and Lactobacillus rhamnosus on the single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida krusei and Candida parapsilosis...
October 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29027827/triggering-receptor-expressed-on-myeloid-cells-in-the-pathogenesis-of-periodontitis-potential-novel-treatment-strategies
#8
Courtney P Rudick, Takanari Miyamoto, Melissa S Lang, Devendra K Agrawal
Periodontal diseases are polymicrobial inflammatory disorders of the tissue, ligament, and bone structures supporting teeth. Periodontitis (inflammation with corresponding loss of attachment) affects 40-50% of adults. Recently, members of the Triggering Receptor on Myeloid Cell (TREM) family have been studied to determine their relationship to these diseases. Areas covered: TREM-1 is a receptor expressed on the surface of PMNs, monocytes, macrophages, dendritic cells, vascular smooth muscle cells, and keratinocytes upregulated in the presence of periodontal inflammation...
October 13, 2017: Expert Review of Clinical Immunology
https://www.readbyqxmd.com/read/29025642/engineered-chimeric-peptides-with-antimicrobial-and-titanium-binding-functions-to-inhibit-biofilm-formation-on-ti-implants
#9
Hongjuan Geng, Yang Yuan, Aidina Adayi, Xu Zhang, Xin Song, Lei Gong, Xi Zhang, Ping Gao
Titanium (Ti) implants have been commonly used in oral medicine. However, despite their widespread clinical application, these implants are susceptible to failure induced by microbial infection due to bacterial biofilm formation. Immobilization of chimeric peptides with antibacterial properties on the Ti surface may be a promising antimicrobial approach to inhibit biofilm formation. Here, chimeric peptides were designed by connecting three sequences (hBD-3-1/2/3) derived from human β-defensin-3 (hBD-3) with Ti-binding peptide-l (TBP-l: RKLPDAGPMHTW) via a triple glycine (G) linker to modify Ti surfaces...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29023743/oral-health-for-an-ageing-population-the-importance-of-a-natural-dentition-in-older-adults
#10
Frauke Müller, Yoshihiro Shimazaki, Febronia Kahabuka, Martin Schimmel
Despite progress in dentistry, tooth loss in old age is still a reality, even more so in long-term-care residents. However, recent trends indicate that natural teeth are lost later in life. Functional decline and age-related pathologies have to be considered in oral health care for this vulnerable population. Retaining and restoring teeth and oral function in elders is important. Tooth loss significantly impairs masticatory performance, which cannot be fully restored by prosthodontic means. Hence an unconscious change in food intake occurs, often involving malnutrition and withdrawal from common meals...
September 2017: International Dental Journal
https://www.readbyqxmd.com/read/29022550/streptococcus-gordonii-pheromone-s-g-cam373-may-influence-the-reservoir-of-antibiotic-resistance-determinants-of-enterococcus-faecalis-origin-in-the-oral-metagenome
#11
Jillian M Mansfield, Paul Herrmann, Amy M Jesionowski, M Margaret Vickerman
Streptococcus gordonii produces a pheromone heptapeptide, s.g.cAM373, which induces a conjugative mating response in Enterococcus faecalis cells carrying the responsive plasmid, pAM373. We investigated the extent of this intergeneric signaling on DNA acquisition by streptococcal species likely to cohabit oral biofilms. E. faecalis/pAM373/pAMS470 cells were incubated with synthetic s.g.cAM373, reverse peptide s.g.cAM373-R, or peptide-free medium and examined for their abilities to transfer plasmid DNA to streptococcal species in the presence of DNase...
October 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29022391/kr-12-a5-is-a-non-cytotoxic-agent-with-potent-antimicrobial-effects-against-oral-pathogens
#12
Karina Sampaio Caiaffa, Loiane Massunari, Marcelle Danelon, Gabriel Flores Abuna, Telma Blanca Lombardo Bedran, Norival Alves Santos-Filho, Denise Madalena Palomari Spolidorio, Natalia Leal Vizoto, Eduardo Maffud Cilli, Cristiane Duque
This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3-1C(V) and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy...
October 12, 2017: Biofouling
https://www.readbyqxmd.com/read/29021252/a-chemical-and-biological-toolbox-for-type-vd-secretion-characterization-of-the-phospholipase-a1-autotransporter-fpla-from-fusobacterium-nucleatum
#13
Michael A Casasanta, Christopher C Yoo, Hans B Smith, Alison J Duncan, Kyla Cochrane, Ann C Varano, Emma Allen-Vercoe, Daniel J Slade
Fusobacterium nucleatum is an oral pathogen that is linked to multiple human infections and colorectal cancer. Strikingly, F. nucleatum achieves virulence in the absence of large, multi-protein secretion systems (Type I, II, III, IV, and VI) which are widely used by Gram-negative bacteria for pathogenesis. By contrast, F. nucleatum strains contain genomic expansions of Type V secreted effectors (autotransporters) that are critical for host cell adherence, invasion, and biofilm formation. Here we present the first characterization of a F...
October 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29020439/non-leachable-imidazolium-incorporated-composite-for-disruption-of-bacterial-clustering-exopolysaccharide-matrix-assembly-and-enhanced-biofilm-removal
#14
Geelsu Hwang, Bernard Koltisko, Xiaoming Jin, Hyun Koo
Surface-grown bacteria and production of an extracellular polymeric matrix modulate the assembly of highly cohesive and firmly attached biofilms, making them difficult to remove from solid surfaces. Inhibition of cell growth and inactivation of matrix-producing bacteria can impair biofilm formation and facilitate removal. Here, we developed a novel non-leachable antibacterial composite with potent antibiofilm activity by directly incorporating polymerizable imidazolium-containing resin (Anti Bacterial Resin with Carbonate linkage; ABR-C) into a methacrylate-based scaffold (ABR Modified Composite; ABR-MC) using an efficient yet simplified chemistry...
October 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29019551/caries-experience-and-salivary-aspects-in-individuals-with-fragile-x-syndrome
#15
Cristhiane Olívia Ferreira do Amaral, Fabiana Gouveia Straioto, Marcelo Henrique Napimoga, Elizabeth Ferreira Martinez
Fragile X syndrome (FXS) is the most common cause of hereditary mental retardation, but studies on the oral health condition of these patients are rare. The aim of this study was to determine the experience of dental caries in individuals with FXS, by examining the saliva profile, oral hygiene, socioeconomic characteristics and use of controlled drugs in these patients. Dental health was estimated using the decayed, missing and filled teeth index (DMF-T) and sialometry, and the pH value and buffering capacity of the saliva, colony forming units of S...
September 28, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/29016613/quantification-of-carious-pathogens-in-the-interdental-microbiota-of-young-caries-free-adults
#16
Denis Bourgeois, Alexandra David, Camille Inquimbert, Paul Tramini, Nicolas Molinari, Florence Carrouel
BACKGROUND: The majority of caries lesions in adults occur on the proximal tooth surfaces of the posterior teeth. A comprehensive study of the composition of the oral microbiota is fundamental for a better understanding of the etiology of interdental caries. METHODS: Twenty-five caries-free subjects (20-35 years old) were enrolled in the study. The interdental biofilm of four interdental sites were collected. The real-time polymerase chain reaction (PCR) methodology were used to quantify (i) the following bacteria: Streptococcus spp...
2017: PloS One
https://www.readbyqxmd.com/read/28989089/fungal-mitochondrial-oxygen-consumption-induces-the-growth-of-strict-anaerobic-bacteria
#17
Joost M Lambooij, Michel A Hoogenkamp, Bernd W Brandt, Marleen M Janus, Bastiaan P Krom
Fungi are commonly encountered as part of a healthy oral ecosystem. Candida albicans is the most often observed and investigated fungal species in the oral cavity. The role of fungi in the oral ecosystem has remained enigmatic for decades. Recently, it was shown that C. albicans, in vitro, influences the bacterial composition of young oral biofilms, indicating it possibly plays a role in increasing diversity in the oral ecosystem. C. albicans favored growth of strictly anaerobic species under aerobic culture conditions...
October 5, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28988196/the-oral-microbiota-in-colorectal-cancer-is-distinctive-and-predictive
#18
Burkhardt Flemer, Ryan D Warren, Maurice P Barrett, Katryna Cisek, Anubhav Das, Ian B Jeffery, Eimear Hurley, Micheal O'Riordain, Fergus Shanahan, Paul W O'Toole
BACKGROUND AND AIMS: Microbiota alterations are linked with colorectal cancer (CRC) and notably higher abundance of putative oral bacteria on colonic tumours. However, it is not known if colonic mucosa-associated taxa are indeed orally derived, if such cases are a distinct subset of patients or if the oral microbiome is generally suitable for screening for CRC. METHODS: We profiled the microbiota in oral swabs, colonic mucosae and stool from individuals with CRC (99 subjects), colorectal polyps (32) or controls (103)...
October 7, 2017: Gut
https://www.readbyqxmd.com/read/28987625/effect-of-toothpaste-containing-arginine-on-dental-plaque-a-randomized-controlled-in-situ-study
#19
Yiyuan Xue, Qian Lu, Yuan Tian, Xuedong Zhou, Lei Cheng, Biao Ren
OBJECTIVES: To evaluate the effects of 8% arginine-containing toothpaste on the dental plaque of no caries (NC) and high caries (HC) individuals in situ. METHODS: 6 no caries (DMFT=0) and 6 high caries (DMFT≥6) individuals wearing a self-developed in situ dental plaque acquisition device were involved in a randomized double-blinded crossover study for 6 weeks: including lead-in (1 week), arginine-free (2 weeks), washout (1 week) and arginine-active (2 weeks) stages...
October 5, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28986373/the-murein-hydrolase-lytf-of-streptococcus-sanguinis-and-the-ecological-consequences-of-competence-development
#20
Nyssa Cullin, Sylvio Redanz, Kirsten J Lampi, Justin Merritt, Jens Kreth
The overall health of the oral cavity is dependent upon a proper homeostasis between health-associated bacterial colonizers and bacteria known to promote dental caries. Streptococcus sanguinis is a health-associated commensal, a known early colonizer of the acquired tooth pellicle, and is naturally competent. We have shown that LytF, a competence-controlled murein hydrolase, is capable of inducing the release of extracellular DNA (eDNA) from oral bacteria. Precipitated and purified LytF were used as treatments against planktonic cultures and biofilms...
October 6, 2017: Applied and Environmental Microbiology
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