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https://www.readbyqxmd.com/read/29141657/local-estrogen-axis-in-the-human-bone-microenvironment-regulates-estrogen%C3%A2-receptor-positive%C3%A2-breast-cancer-cells
#1
Derek F Amanatullah, John S Tamaresis, Pauline Chu, Michael H Bachmann, Nhat M Hoang, Deborah Collyar, Aaron T Mayer, Robert B West, William J Maloney, Christopher H Contag, Bonnie L King
BACKGROUND: Approximately 70% of all breast cancers express the estrogen receptor, and are regulated by estrogen. While the ovaries are the primary source of estrogen in premenopausal women, most breast cancer is diagnosed following menopause, when systemic levels of this hormone decline. Estrogen production from androgen precursors is catalyzed by the aromatase enzyme. Although aromatase expression and local estrogen production in breast adipose tissue have been implicated in the development of primary breast cancer, the source of estrogen involved in the regulation of estrogen receptor-positive (ER+) metastatic breast cancer progression is less clear...
November 15, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29127502/piperacillin-tazobactam-as-alternative-to-carbapenems-for-icu-patients
#2
REVIEW
Benoit Pilmis, Vincent Jullien, Alexis Tabah, Jean-Ralph Zahar, Christian Brun-Buisson
Several studies suggest that alternatives to carbapenems, and particulary beta-lactam/beta-lactamase inhibitor combinations, can be used for therapy of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE)-related infections in non-ICU patients. Little is known concerning ICU patients in whom achieving the desired plasmatic pharmacokinetic/pharmacodynamic (PK/PD) target may be difficult. Also, in vitro susceptibility to beta-lactamase inhibitors might not translate into clinical efficacy. We reviewed the recent clinical studies examining the use of BL/BLI as alternatives to carbapenems for therapy of bloodstream infection, PK/PD data and discuss potential ecological benefit from avoiding the use of carbapenems...
November 10, 2017: Annals of Intensive Care
https://www.readbyqxmd.com/read/29125972/a-biolayer-interferometry-based-enzyme-linked-aptamer-sorbent-assay-for-real-time-and-highly-sensitive-detection-of-pdgf-bb
#3
Shunxiang Gao, Xin Zheng, Jihong Wu
Accurate, fast and sensitive detection of disease-specific protein biomarkers, especially in blood, urine, or other bodily fluids, is an important approach to achieve early disease diagnosis. Platelet-derived growth factor-BB (PDGF-BB), a widely used biomarker, is involved in a substantial number of serious diseases, such as hepatic fibrosis, atherosclerosis, age-related macular degeneration and diabetic eye disease and is often over-expressed in human malignant tumors. Therefore, the development of sensitive and specific detection methods for PDGF-BB is of great importance for the early diagnosis of disease and assessments of patient recovery...
November 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29072708/preparation-of-glycoconjugates-from-unprotected-carbohydrates-for-protein-binding-studies
#4
Christian T Hjuler, Nicolai N Maolanon, Jørgen Sauer, Jens Stougaard, Mikkel B Thygesen, Knud J Jensen
Glycobiology, in particular the study of carbohydrate-protein interactions and the events that follow, has become an important research focus in recent decades. To study these interactions, many assays require homogeneous glycoconjugates in suitable amounts. Their synthesis is one of the methodological challenges of glycobiology. Here, we describe a versatile, three-stage protocol for the formation of glycoconjugates from unprotected carbohydrates, including those purified from natural sources, as exemplified here by rhizobial Nod factors and exopolysaccharide fragments...
November 2017: Nature Protocols
https://www.readbyqxmd.com/read/29069801/effect-of-weekly-or-daily-dosing-regimen-of-gefitinib-in-mouse-models-of-lung-cancer
#5
Qi Zhang, Ruichao Li, Xu Chen, Sang Beom Lee, Jing Pan, Donghai Xiong, Jiaqi Hu, Mark Steven Miller, Eva Szabo, Ronald A Lubet, Yian Wang, Ming You
Gefitinib showed response in phase II clinical trials and with better clinical response in lung cancer with activating mutations in the tyrosine kinase domain of the EGFR. Questions of toxicity and potential dosing regimens impede the use in a prevention setting. This study will provide scientific evidence for the utility of testing and comparing weekly and daily dosing regimens in clinical trials. We employed the adenocarcinoma (AD) and squamous cell carcinoma (SCC) models to compare the efficacy of Gefitinib in daily or weekly dosing regimens...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29065437/basic-bli-adenoma-serrated-international-classification-classification-for-colorectal-polyp-characterization-with-blue-light-imaging
#6
Raf Bisschops, Cesare Hassan, Pradeep Bhandari, Emmanuel Coron, Helmut Neumann, Oliver Pech, Loredana Correale, Alessandro Repici
Backgound and study aim Advanced endoscopic imaging has revolutionized the characterization of lesions during colonoscopy. The aim of this study was to create a new classification for differentiating subcentimetric hyperplastic and adenomatous polyps, and deeply invasive malignant lesions using blue-light imaging (BLI) with high definition, with and without optical magnification, as well as to assess its interobserver concordance. Methods A video library consisting of 48 videos/still images (with/without optical magnification) from 24 histologically verified polyps/cancer with BLI was prospectively created...
October 24, 2017: Endoscopy
https://www.readbyqxmd.com/read/29048160/destruxin-a-induces-and-binds-hsps-in-bombyx-mori-bm12-cells
#7
Huanhuan Zhang, Weina Hu, Miaomiao Xiao, Shiyi Ou, Qiongbo Hu
Destruxin A (DA) is a cyclodepsipeptidic mycotoxin isolated from the entomopathogenic fungus, Metarhizium anisopliae. It has insecticidal activity against host insect's innate immunity system, but the molecular mechanism is not yet elucidated. In our previous experiment, four HSPs (heat shock proteins, BmHSP70-3, BmHSP75, BmHSP83, and BmHSCP) were characterized from the specific protein electrophoretic bands of Bombyx mori Bm12 cell line treated with DA in the test of drug affinity responsive target stability (DARTS), which implied that these HSPs might be kinds of DA-affinity proteins, or DA induces them up-regulated expression...
November 2, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29037466/the-use-of-a-groel-bli-biosensor-to-rapidly-assess-pre-aggregate-populations-for-antibody-solutions-exhibiting-different-stability-profiles
#8
Samantha E Pace, Sangeeta B Joshi, Reza Esfandiary, Robert Stadelman, Steven M Bishop, C R Middaugh, Mark Fisher, David B Volkin
An automated method using biotinylated GroEL-streptavidin biosensors with Bio-Layer Interferometry (GroEL-BLI) was evaluated to detect the formation of transiently formed, pre-aggregate species in various pharmaceutically relevant monoclonal antibody (mAb) samples. The relative aggregation propensity of various IgG1 and IgG4 mAbs was rank-ordered using the GroEL-BLI biosensor method, and the least stable IgG4 mAb was subjected to different stresses including elevated temperatures, acidic pH, and addition of guanidine-HCl...
October 13, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29027077/optimization-of-dendritic-cell-mediated-cytotoxic-t-cell-activation-by-tracking-of-dendritic-cell-migration-using-reporter-gene-imaging
#9
Hongje Lee, Ho Won Lee, You La Lee, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
PURPOSE: The aim of this study is to optimize the dendritic cell (DC)-mediated T-cell activation using reporter gene imaging and flow cytometric analysis in living mice. PROCEDURES: A murine dendritic cell line (DC2.4) co-expressing effluc and Thy1.1 genes were established by transfection with retroviral vectors. Thy1.1 positive cells were sorted by magnetic bead separation system (DC2.4/effluc). Cell proliferation assay and phenotype analysis to determine the effects of gene transduction on the function of dendritic cells between parental DC2...
October 12, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29018414/development-of-a-click-beetle-luciferase-reporter-system-for-enhanced-bioluminescence-imaging-of-listeria-monocytogenes-analysis-in-cell-culture-and-murine-infection-models
#10
Sadeeq Ur Rahman, Michael Stanton, Pat G Casey, Angela Spagnuolo, Giuliano Bensi, Colin Hill, Kevin P Francis, Mark Tangney, Cormac G M Gahan
Listeria monocytogenes is a Gram-positive facultative intracellular pathogen that is widely used as a model organism for the analysis of infection biology. In this context, there is a current need to develop improved reporters for enhanced bioluminescence imaging (BLI) of the pathogen in infection models. We have developed a click beetle red luciferase (CBR-luc) based vector (pPL2CBR(opt)) expressing codon optimized CBR-luc under the control of a highly expressed Listerial promoter (PHELP) for L. monocytogenes and have compared this to a lux-based system expressing bacterial luciferase for BLI of the pathogen using in vitro growth experiments and in vivo models...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29016143/nuclear-and-fluorescent-labeled-pd-1-liposome-dox-64-cu-irdye800cw-allows-improved-breast-tumor-targeted-imaging-and-therapy
#11
Yang Du, Xiaolong Liang, Yuan Li, Ting Sun, Zhengyu Jin, Huadan Xue, Jie Tian
The overexpression of programmed cell death-1 (PD-1) in tumors as breast cancer makes it a possible target for cancer imaging and therapy. Advances in molecular imaging, including radionuclide imaging and near-infrared fluorescence (NIRF) imaging, enable the detection of tumors with high sensitivity. In this study, we aim to develop a novel PD-1 antibody targeted positron emission tomography (PET) and NIRF labeled liposome loaded with doxorubicin (DOX) and evaluate its application for in vivo cancer imaging and therapy...
November 6, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28990786/longitudinal-monitoring-of-antibody-responses-against-tumor-cells-using-magneto-nanosensors-with-a-nanoliter-of-blood
#12
Jung-Rok Lee, Carmel T Chan, Daniel Ruderman, Hui-Yen Chuang, Richard S Gaster, Michelle Atallah, Parag Mallick, Scott W Lowe, Sanjiv S Gambhir, Shan X Wang
Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magnetoresistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum. Combining in vitro serologic measurements with in vivo imaging techniques, we investigated the role of the antibody response in the regression of firefly luciferase (FL)-labeled lymphoma cells in spleen, kidney, and lymph nodes in a syngeneic Burkitt's lymphoma mouse model...
October 20, 2017: Nano Letters
https://www.readbyqxmd.com/read/28985608/the-role-of-neutrophil-gelatinase-associated-lipocalin-ngal-in-the-detection-of-blast-lung-injury-in-a-military-population
#13
Andrew Lumley, Erik Osborn, Adrian Mellor, Elise LaCroix, George Johnson, Mary Wallace, Mike Stacey, Jason E Smith, David Woods
PURPOSE: To study the relationship between serum neutrophil gelatinase-associated lipocalin (NGAL) and military blast and gunshot wound (GSW) to establish whether potential exists for NGAL as a biomarker for blast lung injury (BLI). METHOD: Patients from the intensive care unit (ICU) of the Role 3 Medical Treatment Facility at Camp Bastion, Helmand Province, Afghanistan were studied over a five month period commencing in 2012. Age, mechanism, trauma injury severity score (TRISS) and serum NGAL were recorded on ICU admission (NGAL1)...
September 1, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28970009/comparison-of-different-immunoassay-methods-to-detect-human-anti-drug-antibody-using-the-who-erythropoietin-antibody-reference-panel-for-analytes
#14
Hiroko Shibata, Kazuko Nishimura, Chizuru Miyama, Minoru Tada, Takuo Suzuki, Yoshiro Saito, Akiko Ishii-Watabe
Development of an appropriate assay to detect anti-drug antibody (ADA) is important for assessing immunogenicity to therapeutic protein products. However, characterizing ADA assay methods is difficult because human ADA as a reference standard is not available in most cases. We compared the analytical performance of three ligand-binding assay methods for ADA, namely, surface plasmon resonance (SPR), electrochemiluminescence (ECL), and biolayer interferometry (BLI) methods, by using the anti-erythropoietin (EPO) monoclonal antibody reference panel developed by the World Health Organization (WHO) in 2015...
September 29, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28960132/in-vivo-monitoring-of-infectious-diseases-in-living-animals-using-bioluminescence-imaging
#15
Asheesh Gupta, Pinar Avci, Mahdi Karimi, Magesh Sadasivam, Wanessa C Antunes-Melo, Elisa Carrasco, Michael R Hamblin
Traditional methods of localizing and quantifying the presence of pathogenic microorganisms in living experimental animal models of infections have mostly relied on sacrificing the animals, dissociating the tissue and counting the number of colony forming units. However the discovery of several varieties of the light producing enzyme, luciferase, and the genetic engineering of bacteria, fungi, parasites and mice to make them emit light, either after administration of the luciferase substrate, or in the case of the bacterial lux operon without any exogenous substrate, has provided a new alternative...
September 29, 2017: Virulence
https://www.readbyqxmd.com/read/28955347/blister-regulates-polycomb-target-genes-represses-stress-regulated-genes-and-promotes-stress-responses-in-arabidopsis-thaliana
#16
Julia A Kleinmanns, Nicole Schatlowski, David Heckmann, Daniel Schubert
HIGHLIGHTS The PRC2 interacting protein BLISTER likely acts downstream of PRC2 to silence Polycomb target genes and is a key regulator of specific stress responses in Arabidopsis. Polycomb group (PcG) proteins are key epigenetic regulators of development. The highly conserved Polycomb repressive complex 2 (PRC2) represses thousands of target genes by trimethylating H3K27 (H3K27me3). Plant specific PcG components and functions are largely unknown, however, we previously identified the plant-specific protein BLISTER (BLI) as a PRC2 interactor...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28954304/magnified-endoscopic-observation-of-small-depressed-gastric-lesions-using-linked-color-imaging-with-indigo-carmine-dye
#17
Yoshiyasu Kitagawa, Taro Hara, Dai Ikebe, Rino Nankinzan, Hideyuki Takashiro, Ryosuke Kobayashi, Kazuyoshi Nakamura, Taketo Yamaguchi, Takuto Suzuki
Background and study aims Magnifying linked color imaging with indigo carmine dye (M-Chromo-LCI) enables sterically enhanced and color image-magnified observation of the superficial gastric mucosa. This study investigated the usefulness of M-Chromo-LCI for the differential diagnosis of gastric lesions. Patients and methods 100 consecutive small depressed lesions were examined with conventional white-light imaging (C-WLI), magnifying blue-laser imaging (M-BLI), and M-Chromo-LCI. Endoscopic images were reviewed by three experts and three non-experts...
September 27, 2017: Endoscopy
https://www.readbyqxmd.com/read/28947900/magnifying-endoscopy-with-blue-laser-imaging-improves-the-microstructure-visualization-in-early-gastric-cancer-comparison-of-magnifying-endoscopy-with-narrow-band-imaging
#18
Reiko Kimura-Tsuchiya, Osamu Dohi, Yasuko Fujita, Nobuaki Yagi, Atsushi Majima, Yusuke Horii, Tomoko Kitaichi, Yuriko Onozawa, Kentaro Suzuki, Akira Tomie, Tetsuya Okayama, Naohisa Yoshida, Kazuhiro Kamada, Kazuhiro Katada, Kazuhiko Uchiyama, Takeshi Ishikawa, Tomohisa Takagi, Osamu Handa, Hideyuki Konishi, Mitsuo Kishimoto, Yuji Naito, Akio Yanagisawa, Yoshito Itoh
BACKGROUNDS: Magnifying endoscopy with blue laser imaging (ME-BLI) for diagnosis of early gastric cancer (EGC) is as effective as magnifying endoscopy with narrow-band imaging (ME-NBI). However, there are different EGCs in microstructure visualization between ME-BLI and ME-NBI. This study aimed to clarify the pathological features of the EGCs, in which microstructure visualization was different between ME-NBI and ME-BLI. METHODS: EGCs were classified into groups A (irregular microsurface pattern (MSP) in ME-BLI and absent MSP in ME-NBI), B (irregular MSP in two modalities), or C (absent MSP in two modalities), according to the vessel plus surface classification...
2017: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/28868553/origins-of-the-enhanced-affinity-of-rna-protein-interactions-triggered-by-rna-phosphorodithioate-backbone-modification
#19
Xianbin Yang, N Dinuka Abeydeera, Feng-Wu Liu, Martin Egli
The well-characterized interaction between the MS2 coat protein and its cognate RNA hairpin was used to evaluate changes in affinity as a result of phosphorodithioate (PS2) replacing phosphate by biolayer interferometry (BLI). A structure-based analysis of the data provides insights into the origins of the enhanced affinity of RNA-protein interactions triggered by the PS2 moiety.
September 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28861163/image-guided-stem-cells-with-functionalized-self-assembling-peptide-nanofibers-for-treatment-of-acute-myocardial-infarction-in-a-mouse-model
#20
Xiao Li, Ying-Ying Chen, Xiu-Mei Wang, Kai Gao, Yun-Zhou Gao, Jian Cao, Zhuo-Li Zhang, Jing Lei, Zheng-Yu Jin, Yi-Ning Wang
AIM: To investigate the survival of bone marrow mesenchymal stem cells (BMSCs) and the therapeutic effect for acute myocardial infarction (AMI) after co-transplantation with the functionalized self-assembling peptide nanofiber RAD/PRG or RAD/KLT. METHODS: AMI of balb/c mice was induced. Mice were randomly divided into four groups, and received treatment of phosphate buffered saline (PBS) (Group A), GFP/Fluc-BMSCs (Group B), GFP/Fluc-BMSCs + RAD/PRG (Group C), and GFP/Fluc-BMSCs + RAD/KLT (Group D), respectively...
2017: American Journal of Translational Research
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