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https://www.readbyqxmd.com/read/29774076/the-human-somatostatin-receptor-type-2-as-an-imaging-and-suicide-reporter-gene-for-pluripotent-stem-cell-derived-therapy-of-myocardial-infarction
#1
Katrien Neyrinck, Natacha Breuls, Bryan Holvoet, Wouter Oosterlinck, Esther Wolfs, Hubert Vanbilloen, Olivier Gheysens, Robin Duelen, Willy Gsell, Ivo Lambrichts, Uwe Himmelreich, Catherine M Verfaillie, Maurilio Sampaolesi, Christophe M Deroose
Rationale: Pluripotent stem cells (PSCs) are being investigated as a cell source for regenerative medicine since they provide an infinitive pool of cells that are able to differentiate towards every cell type of the body. One possible therapeutic application involves the use of these cells to treat myocardial infarction (MI), a condition where billions of cardiomyocytes (CMs) are lost. Although several protocols have been developed to differentiate PSCs towards CMs, none of these provide a completely pure population, thereby still posing a risk for neoplastic teratoma formation...
2018: Theranostics
https://www.readbyqxmd.com/read/29765423/comparison-of-teratoma-formation-between-embryonic-stem-cells-and-parthenogenetic-embryonic-stem-cells-by-molecular-imaging
#2
Hongyan Tao, Xiaoniao Chen, Anbang Wei, Xianghe Song, Weiqiang Wang, Lu Liang, Qinjun Zhao, Zhibo Han, Zhongchao Han, Xiaojing Wang, Zongjin Li
With their properties of self-renewal and differentiation, embryonic stem (ES) cells hold great promises for regenerative therapy. However, teratoma formation and ethical concerns of ES cells may restrict their potential clinical applications. Currently, parthenogenetic embryonic stem (pES) cells have attracted the interest of researchers for its self-renewing and pluripotent differentiation while eliciting less ethic concerns. In this study, we established a model with ES and pES cells both stably transfected with a double-fusion reporter gene containing renilla luciferase (Rluc) and red fluorescent protein (RFP) to analyze the mechanisms of teratoma formation...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29761416/bioluminescence-imaging-of-transplanted-mesenchymal-stem-cells-by-overexpression-of-hepatocyte-nuclear-factor4%C3%AE-tracking-biodistribution-and-survival
#3
Peiyi Xie, Xiaojun Hu, Dan Li, Sidong Xie, Zhiyang Zhou, Xiaochun Meng, Hong Shan
PURPOSE: The purposes of this study were to construct immortalized human bone marrow mesenchymal stem cells (UE7T-13) with overexpression of the hepatocyte nuclear factor4α (hHNF4α) and luciferase2-mKate2 dual-fusion reporter gene, further investigate their impact on treating acute liver injury (ALI) in rats, and track their biodistribution and survival by bioluminescence imaging (BLI). PROCEDURES: The hHNF4α and luciferase2-mKate2 genes were transduced by a lentiviral vector into UE7T-13 cells (named E7-hHNF4α-R cells), and expression was verified by immunofluorescence, RT-PCR, and flow cytometry...
May 14, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29754144/mgatp-hydrolysis-destabilizes-the-interaction-between-subunit-h-and-yeast-v1-atpase-highlighting-h-s-role-in-v-atpase-regulation-by-reversible-disassembly
#4
Stuti Sharma, Rebecca A Oot, Stephan Wilkens
Vacuolar H+-ATPases (V-ATPases; V1Vo-ATPases) are rotary motor proton pumps that acidify intracellular compartments and in some tissues, the extracellular space. V-ATPase is regulated by reversible disassembly into autoinhibited V1-ATPase and Vo proton channel sectors. An important player in V-ATPase regulation is subunit H, which binds at the interface of V1 and Vo. H is required for MgATPase activity in holo V-ATPase, but also for stabilizing the MgADP inhibited state in membrane detached V1. However, how H fulfills these two functions is poorly understood...
May 12, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29740437/targeting-and-therapy-of-glioblastoma-in-a-mouse-model-using-exosomes-derived-from-natural-killer-cells
#5
Liya Zhu, Ji Min Oh, Prakash Gangadaran, Senthilkumar Kalimuthu, Se Hwan Baek, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Objective: Glioblastoma is a highly aggressive primary brain tumor that is resistant to radiotherapy and chemotherapy. Natural killer (NK) cells have been used to treat incurable cancers. Recent studies have investigated the effectiveness of NK-cell-derived exosomes (NK-Exo) for treating incurable cancers such as melanoma, leukemia, and neuroblastoma; however, NK-Exo have not been used to treat glioblastoma. In the present study, we investigated the antitumor effects of NK-Exo against aggressive glioblastoma both in vitro and in vivo and determined the tumor-targeting ability of NK-Exo by performing fluorescence imaging...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29723251/spatiotemporal-assessment-of-spontaneous-metastasis-formation-using-multimodal-in-vivo-imaging-in-her2-and-triple-negative-metastatic-breast-cancer-xenograft-models-in-mice
#6
Inga B Fricke, Raquel De Souza, Lais Costa Ayub, Giulio Francia, Robert Kerbel, David A Jaffray, Jinzi Zheng
BACKGROUND: Preclinical breast cancer models recapitulating the clinical course of metastatic disease are crucial for drug development. Highly metastatic cell lines forming spontaneous metastasis following orthotopic implantation were previously developed and characterized regarding their biological and histological characteristics. This study aimed to non-invasively and longitudinally characterize the spatiotemporal pattern of metastasis formation and progression in the MDA-MB-231-derived triple negative LM2-4 and HER2+ LM2-4H2N cell lines, using bioluminescence imaging (BLI), contrast enhanced computed tomography (CT), fluorescence imaging, and 2-deoxy-2-[fluorine-18]fluoro-D-glucose positron emission tomography ([18F]FDG-PET)...
2018: PloS One
https://www.readbyqxmd.com/read/29721927/estimations-of-retinal-blue-light-irradiance-values-and-melatonin-suppression-indices-through-clear-and-yellow-tinted-intraocular-lenses
#7
Masaki Tanito, Ichiya Sano, Tsutomu Okuno, Yoshihisa Ishiba, Akihiro Ohira
Spectral transmittance values in the wavelength range of 300 to 800 nanometers were measured using a spectrophotometer for 18 intraocular lenses (IOLs) including clear (ZCB00) and yellow-tinted (ZCB00V, both from AMO Japan) IOLs with three different lens powers. Also measured were the blue-light irradiance (BLI) values, which might reflect retinal damage caused by sunlight, and the melatonin suppression indices (MSIs), which might reflect the nonvisual photoreception function, through these IOLs. The BLIs (in mWcm-2 ) calculated were 7...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29706245/non-invasive-bioluminescence-imaging-as-a-standardized-assessment-measure-in-mouse-models-of-dermal-inflammation
#8
Martine Catharina Josephine Hofmann, Mike Schmidt, Olga Arne, Gerd Geisslinger, Michael John Parnham, Natasja Maria Wilhelmina Johanna de Bruin
BACKGROUND: Myeloperoxidase is used as a marker and diagnostic tool for inflammatory processes. Hypochlorous acid produced by myeloperoxidase oxidizes luminol to produce light. By injecting luminol into experimental animals, inflammatory processes can be tracked in real-time by bioluminescence imaging (BLI). OBJECTIVE: We aimed to establish BLI as a standardized assessment measure in three mouse models of dermal inflammation. METHODS: Oxazolone-induced delayed-type-hypersensitivity (DTH) (acute), a model for dermatitis, imiquimod (IMQ) (sub-chronic) model for psoriasis and the (chronic) bleomycin model for scleroderma were used...
April 23, 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29704644/effect-of-%C3%AE-elemene-on-the-kinetics-of-intracellular-transport-of-d-luciferin-potassium-salt-abc-substrate-in-doxorubicin-resistant-breast-cancer-cells-and-the-associated-molecular-mechanism
#9
Chao-Yuan Tang, Li-Xin Zhu, Jian-Dong Yu, Zhi Chen, Man-Cang Gu, Chao-Feng Mu, Qi Liu, Yang Xiong
In order to explore the mechanism of the reversing multidrug resistance (MDR) phenotypes by β-elemene (β-ELE) in doxorubicin (DOX)-resistant breast cancer cells (MCF-7/DOX), both the functionality and quantity of the ABC transporters in MCF-7/DOX were studied. Bioluminescence imaging (BLI) was used to study the efflux of d-luciferin potassium salt, the substrate of ATP-binding cassette transporters (ABC transporters), in MCF-7/DOX cells treated by β-ELE. At the same time three major ABC transport proteins and genes-related MDR, P-glycoprotein (P-gp, ABCB1) and multidrug resistance-associated protein 1 (MRP, ABCC1) as well as breast cancer resistance protein (BCRP, ABCG2) were analyzed by q-PCR and Western blot...
April 25, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29691368/a-novel-sigb-q225p-mutation-in-staphylococcus-aureus-retains-virulence-but-promotes-biofilm-formation
#10
Hui Liu, Weilong Shang, Zhen Hu, Ying Zheng, Jizhen Yuan, Qiwen Hu, Huagang Peng, Xinyu Cai, Li Tan, Shu Li, Junmin Zhu, Ming Li, Xiaomei Hu, Renjie Zhou, Xiancai Rao, Yi Yang
Staphylococcus aureus is an important pathogen that produces abundant virulence factors, which cause various diseases that burden human health worldwide. The stress response regulon called sigma factor B (SigB) is a well-characterized global regulator that is involved in the regulation of S. aureus virulence, pigmentation, and biofilm formation. However, the regulatory network upon SigB in S. aureus is incompletely described. Here, we identified a novel substitution mutation, SigB(Q225P), which contributed the nonpigmented phenotype of S...
April 25, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29685806/diagnostic-ability-of-blue-laser-imaging-combined-with-magnifying-endoscopy-for-early-esophageal-cancer
#11
Wenxiu Diao, Xu Huang, Lei Shen, Zhi Zeng
BACKGROUND: Blue laser imaging (BLI) is a new image-enhanced endoscopy technique that utilizes a laser light source developed for narrow-band light observation. AIMS: To evaluate the value of BLI combined with magnifying endoscopy (M-BLI) for the diagnosis of early esophageal cancers (EECs). METHODS: This single-center prospective study analyzed 149 patients with focal esophageal lesions detected with white light endoscopy (WLE) at Renmin Hospital of Wuhan University between April 2015 and June 2017...
March 30, 2018: Digestive and Liver Disease
https://www.readbyqxmd.com/read/29682209/-89-zr-nimotuzumab-for-immunopet-imaging-of-epidermal-growth-factor-receptor-i
#12
Rufael Chekol, Viswas Raja Solomon, Elahe Alizadeh, Wendy Bernhard, Darrell Fisher, Wayne Hill, Kris Barreto, John Francis DeCoteau, Angel Casaco Parada, Clarence Ronald Geyer, Humphrey Fonge
Rationale: Epidermal growth factor receptor (EGFR) upregulation is associated with enhanced proliferation and drug resistance in a number of cancers. Nimotuzumab is a humanized monoclonal antibody with high affinity for EGFR. The objective of this study was to determine if 89 Zr-DFO-nimotuzumab could be suitable for human use as a PET probe for quantifying EGFR in vivo . Methods: To evaluate the pharmacokinetics, biodistribution, microPET imaging, radiation dosimetry, and normal tissue toxicity in tumor and non-tumor bearing mice of 89 Zr-desferoxamine-nimotuzumab (89 Zr-DFO-nimotuzumab) of a product prepared under GMP conditions...
March 30, 2018: Oncotarget
https://www.readbyqxmd.com/read/29671177/tri-modal-in-vivo-imaging-of-pancreatic-islets-transplanted-subcutaneously-in-mice
#13
Sayuan Liang, Karim Louchami, Bryan Holvoet, Rein Verbeke, Christophe M Deroose, Bella Manshian, Stefaan J Soenen, Ine Lentacker, Uwe Himmelreich
PURPOSE: Transplantation of pancreatic islets (PIs) is a promising therapeutic approach for type 1 diabetes. The main obstacle for this strategy is that the outcome of islet engraftment depends on the engraftment site. It was our aim to develop a strategy for using non-invasive imaging techniques to assess the location and fate of transplanted PIs longitudinally in vivo. PROCEDURES: In order to overcome the limitations of individual imaging techniques and cross-validate findings by different modalities, we have combined fluorine magnetic resonance imaging (F-19 MRI), fluorescence imaging (FLI), and bioluminescent imaging (BLI) for studying subcutaneously transplanted PIs and beta cell-like cells (INS-1E cell line) in vivo...
April 18, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29655989/in-vivo-migration-of-mesenchymal-stem-cells-to-burn-injury-sites-and-their-therapeutic-effects-in-a-living-mouse-model
#14
Eun Jung Oh, Ho Won Lee, Senthilkumar Kalimuthu, Tae Jung Kim, Hyun Mi Kim, Se Hwan Baek, Liya Zhu, Ji Min Oh, Seung Hyun Son, Ho Yun Chung, Byeong-Cheol Ahn
Mesenchymal stem cell (MSC)-based therapy has emerged as a promising therapeutic strategy for tissue regeneration and repair. In this study, we non-invasively monitored the tracking of MSCs toward burn injury sites using MSCs expressing firefly luciferase (Fluc) gene in living mice, and evaluated the effects of the MSCs at the injury site. Murine MSCs co-expressing Fluc and green fluorescent protein (GFP) were established using a retroviral system (referred to as MSC/Fluc). To evaluate the ability of MSC migration toward burn injury sites, cutaneous burn injury was induced in the dorsal skin of mice...
April 12, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29652608/immunomodulatory-role-of-stem-cell-from-human-exfoliated-deciduous-teeth-on-periodontal-regeneration
#15
Xianling Gao, Zongshan Shen, Meiliang Guan, Qiting Huang, Lingling Chen, Wei Qin, Xiaohu Ge, Haijia Chen, Yin Xiao, Zhengmei Lin
Periodontitis is initiated by the infection of periodontal bacteria and subsequent tissue inflammation due to immunoreaction, eventually leading to periodontal apparatus loss. Stem cells from human exfoliated deciduous teeth (SHEDs) have exhibited beneficial characteristics in dental tissue regeneration. However, the immunomodulatory functions of SHEDs have not been elucidated in the context of periodontitis treatment. In this study, we investigated the potential immunomodulatory effects of SHEDs on experimental periodontitis and demonstrated that multi-dose delivery of SHEDs led to periodontal tissue regeneration...
March 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29651576/fluorescence-molecular-imaging-and-tomography-of-matrix-metalloproteinase-activatable-near-infrared-fluorescence-probe-and-image-guided-orthotopic-glioma-resection
#16
Li Li, Yang Du, Xinjian Chen, Jie Tian
PURPOSE: Malignant gliomas are major causes of cancer-related mortality and morbidity. Traditional surgery usually leads to incomplete resection of gliomas resulting in the high incidence of tumor recurrence. Advanced medical imaging technology, such as fluorescence imaging-guided surgery, combined with tumor-specific imaging probes allows the identification of tumor margins and improved surgery. However, there are two pressing issues that need to be addressed: first, few fluorescence imaging probes can specifically target gliomas; second, fluorescence molecular imaging (FMI) cannot get the in-depth information of deep-seated gliomas; both of which affect the complete removal of the gliomas...
April 12, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29649959/whole-brain-magnetic-resonance-spectroscopic-determinants-of-functional-outcomes-in-pediatric-moderate-severe-traumatic-brain-injury
#17
Talin Babikian, Jeffry R Alger, Monica U Ellis-Blied, Christopher C Giza, Emily Dennis, Alexander Olsen, Richard Mink, Christopher Babbitt, Jeffrey Johnson, Paul Thompson, Robert F Asarnow
Diffuse axonal injury contributes to the long-term functional morbidity observed following pediatric moderate/severe traumatic brain injury (msTBI). Whole-Brain Proton Magnetic Resonance Echo-Planar Spectroscopic Imaging was used to measure the neurometabolite levels in the brain to delineate the course of disruption/repair during the first year post msTBI. The association between metabolite biomarkers and functional measures (cognitive functioning and CC function assessed by interhemispheric transfer time (IHTT) using an Event Related Potential paradigm) was also explored...
April 13, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29596454/classification-of-atrophic-mucosal-patterns-on-blue-laser-imaging-for-endoscopic-diagnosis-of-helicobacter-pylori-related-gastritis-a-retrospective-observational-study
#18
Yoshiyuki Nishikawa, Yoshio Ikeda, Hidehiro Murakami, Shin-Ichiro Hori, Kaori Hino, Chise Sasaki, Megumi Nishikawa
BACKGROUND: Atrophic gastritis can be classified according to characteristic mucosal patterns observed by Blue LASER Imaging (BLI) in a medium-range to distant view. AIMS: To facilitate the endoscopic diagnosis of Helicobacter pylori (HP)-related gastritis, we investigated whether atrophic mucosal patterns correlated with HP infection based on the image interpretations of three endoscopists blinded to clinical features. METHODS: This study included 441 patients diagnosed as having atrophic gastritis by upper gastrointestinal endoscopy at Nishikawa Gastrointestinal Clinic between April 1, 2015 and March 31, 2016...
2018: PloS One
https://www.readbyqxmd.com/read/29578501/capturing-the-interaction-kinetics-of-an-ion-channel-protein-with-small-molecules-by-the-bio-layer-interferometry-assay
#19
Bo Han, Man Zhang, Peng Sun, Shangwei Hou
The bio-layer interferometry (BLI) assay is a valuable tool for measuring protein-protein and protein-small molecule interactions. Here, we first describe the application of this novel label-free technique to study the interaction of human EAG1 (hEAG1) channel proteins with the small molecule PIP2. hEAG1 channel has been recognized as potential therapeutic target because of its aberrant overexpression in cancers and a few gain-of-function mutations involved in some types of neurological diseases. We purified hEAG1 channel proteins from a mammalian stable expression system and measured the interaction with PIP2 by BLI...
March 7, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29575236/dmc-is-not-better-than-tmz-on-intracranial-anti-glioma-effects
#20
Lei Shi, Guan Sun
Previous studies showed Demethoxycurcumin (DMC) has stronger anti-glioma and anti-GSCs effects both in vitro and in vivo. In addition, DMC seems to be lower toxicity than TMZ on nude mice. However, this conclusion was confirmed to be wrong in this study. We have evaluated the antitumor efficacy of DMC or TMZ treatment by an orthotopic glioblastoma xenograft model. Nude mice were injected with U87MG-luc cells in the caudate nucleus of the brain and treated with DMC (30 mg/kg q.d.) or TMZ (10 mg/kg q.d.) by intraperitoneal injection...
March 25, 2018: Journal of Cellular Biochemistry
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