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Yun Xiang, Zai-Hua Wang, Ping Cai, Zhen Zhang
OBJECTIVE: To study the effect of β-lactamase (BLs) detection and β-lactam/β-lactamase inhibitor (BL/BLI) on the incidence of antibiotic-associated diarrhea (AAD) in children with severe bacterial pneumonia. METHODS: The clinical data of the children with bacterial severe pneumonia were retrospectively studied. Of all the patients, 248 using amoxicillin/clavulanate but without BLs detection and 323 using amoxicillin (BLs negative) or amoxicillin/clavulanate (BLs positive) were used as the amoxicillin group; 208 patients using piperacillin/tazobactam but without BLs detection and 291 patients using piperacillin (BLs negative) or piperacillin/tazobactam (BLs positive) were used as the piperacillin group; and 191 patients using cefoperazone/sulbactam but without BLs detection and 341 patients using cefoperazone (BLs negative) or cefoperazone/sulbactam (BLs positive) were used as the cefoperazone group...
October 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
Akira Tomie, Osamu Dohi, Nobuaki Yagi, Hiroaki Kitae, Atsushi Majima, Yusuke Horii, Tomoko Kitaichi, Yuriko Onozawa, Kentaro Suzuki, Reiko Kimura-Tsuchiya, Tetsuya Okayama, Naohisa Yoshida, Kazuhiro Kamada, Kazuhiro Katada, Kazuhiko Uchiyama, Takeshi Ishikawa, Tomohisa Takagi, Osamu Handa, Hideyuki Konishi, Yuji Naito, Yoshito Itoh
Background/Aims. The aim of this study was to evaluate the endoscopic recognition of esophageal squamous cell carcinoma (ESCC) using four different methods (Olympus white light imaging (O-WLI), Fujifilm white light imaging (F-WLI), narrow band imaging (NBI), and blue laser imaging- (BLI-) bright). Methods. We retrospectively analyzed 25 superficial ESCCs that had been examined using the four different methods. Subjective evaluation was provided by three endoscopists as a ranking score (RS) of each image based on the ease of detection of the cancerous area...
2016: Gastroenterology Research and Practice
MeiJuan Song, Qi Lv, XiuWei Zhang, Juan Cao, ShuLi Sun, PeiXin Xiao, ShiKe Hou, Hui Ding, ZiQuan Liu, WenLong Dong, JinQiang Wang, Xue Wang, ZhiGuang Sun, Man Tian, HaoJun Fan
Multiple preclinical evidences have supported the potential value of mesenchymal stem cells (MSCs) for treatment of acute lung injury (ALI). However, few studies focus on the dynamic tropism of MSCs in animals with acute lung injury. In this study, we track systemically transplanted human bone marrow-derived mesenchymal stem cells (hBMSCs) in NOD/SCID mice with smoke inhalation injury (SII) through bioluminescence imaging (BLI). The results showed that hBMSCs systemically delivered into healthy NOD/SCID mouse initially reside in the lungs and then partially translocate to the abdomen after 24 h...
2016: Stem Cells International
Carlos Eduardo Bezerra Gomes, Dhiogo Gonçalves Cavalcante, José Eduardo Girão Filho, Flávio Nogueira da Costa, Sérgio Luís da Silva Pereira
BACKGROUND: Plaque-associated gingivitis is a prevalent disease and research in its treatment using herbal agents must be encouraged to verify which would be a useful addition to the current range or chemotherapeutic treatment options. AIMS: The aim of this study was to evaluate the clinical effect of a mouth rinse containing 10% Anacardium occidentale (AO) Linn., a typical plant commonly found in the Northeast Region of Brazil, on the reduction of plaque and gingivitis in comparison to a gold-standard chemotherapeutic agent...
July 2016: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
Zachary M Parker, Tracy Jo Pasieka, George A Parker, David A Leib
: The interferon (IFN) response to viral pathogens is critical for host survival. In humans and mouse models, defects in IFN responses can result in lethal herpes simplex type-1 (HSV-1) infections, usually from encephalitis. Although rare, HSV-1 can also cause fulminant hepatic failure which is often fatal. While herpes simplex encephalitis has been extensively studied, HSV-1 generalized infections and subsequent acute liver failure are less well understood. We previously demonstrated that IFNαβγR(-/-) mice are exquisitely susceptible to liver infection following corneal infection with HSV-1...
September 28, 2016: Journal of Virology
Charlotte A Hall, Helen C Flick-Smith, Sarah V Harding, Helen S Atkins, Richard W Titball
Bioluminescence imaging (BLI) enables real-time, non-invasive tracking of infection in vivo and longitudinal infection studies. In this study, a bioluminescent F. tularensis SCHU S4-lux strain was used to develop an inhalational infection model in BALB/c mice. Mice were infected intranasally and the progression of infection was monitored in real time using BLI. A bioluminescent signal was detectable from 3 days post-infection (dpi), initially in the spleen, then in the liver and lymph nodes before finally becoming systemic...
September 26, 2016: Antimicrobial Agents and Chemotherapy
Dongmin Seo, Sung-Ho Paek, Sangwoo Oh, Sungkyu Seo, Se-Hwan Paek
The incidence of diabetes is continually increasing, and by 2030, it is expected to have increased by 69% and 20% in underdeveloped and developed countries, respectively. Therefore, glucose sensors are likely to remain in high demand in medical device markets. For the current study, we developed a needle-type bio-layer interference (BLI) sensor that can continuously monitor glucose levels. Using dialysis procedures, we were able to obtain hypoglycemic samples from commercial human serum. These dialysis-derived samples, alongside samples of normal human serum were used to evaluate the utility of the sensor for the detection of the clinical interest range of glucose concentrations (70-200 mg/dL), revealing high system performance for a wide glycemic state range (45-500 mg/dL)...
2016: Sensors
Xiaotian Sun, Tenghui Dong, Yiliang Bi, Min Min, Wei Shen, Yang Xu, Yan Liu
Endoscopy has been widely used in diagnosing gastrointestinal mucosal lesions. However, there are still lack of objective endoscopic criteria. Linked color imaging (LCI) is newly developed endoscopic technique which enhances color contrast. Thus, we investigated the clinical application of LCI and further analyzed pixel brightness for RGB color model. All the lesions were observed by white light endoscopy (WLE), LCI and blue laser imaging (BLI). Matlab software was used to calculate pixel brightness for red (R), green (G) and blue color (B)...
2016: Scientific Reports
Chuang Xiao, Wu Lian, Lutan Zhou, Na Gao, Li Xu, Jun Chen, Mingyi Wu, Wenlie Peng, Jinhua Zhao
Fucosylated glycosaminoglycan (FG) is a structurally novel glycosaminoglycan derivative, and it has potent anticoagulant activity. Depolymerized FG (dFG) is a selective factor Xase (FXase, FIXa-FVIIIa complex) inhibitor and it has antithrombotic action without major bleeding risks. In this study, we report the effects of dFG-3 (Mw ~14kDa) on the catalysis rates of factor IIa (FIIa), factor Xa (FXa) and factor IXa (FIXa) inhibition by antithrombin (AT), and the kinetic of the interactions between coagulation proteases or inhibitors and dFG-3 were also studied using biolayer interferometry (BLI) technology...
October 2016: Thrombosis Research
Juan Cao, Shike Hou, Hui Ding, Ziquan Liu, Meijuan Song, Xiaojing Qin, Xue Wang, Mengyang Yu, Zhiguang Sun, Jinyang Liu, Shuli Sun, Peixin Xiao, Qi Lv, Haojun Fan
Recently, mesenchymal stem cells (MSCs) are increasingly used as a panacea for multiple types of disease short of effective treatment. Dozens of clinical trials published demonstrated strikingly positive therapeutic effects of MSCs. However, as a specific agent, little research has focused on the dynamic distribution of MSCs after in vivo administration. In this study, we track systemically transplanted allogeneic bone marrow mesenchymal stem cells (BMSCs) in normal rats through bioluminescence imaging (BLI) in real time...
2016: Stem Cells International
D Verzijl, T Riedl, P W H I Parren, A F Gerritsen
Biolayer interferometry (BLI) is a well-established optical label-free technique to study biomolecular interactions. Here we describe for the first time a cell-based BLI (cBLI) application that allows label-free real-time monitoring of signal transduction in living cells. Human A431 epidermoid carcinoma cells were captured onto collagen-coated biosensors and serum-starved, followed by exposure to agonistic compounds targeting various receptors, while recording the cBLI signal. Stimulation of the epidermal growth factor receptor (EGFR) with EGF, the β2-adrenoceptor with dopamine, or the hepatocyte growth factor receptor (HGFR/c-MET) with an agonistic antibody resulted in distinct cBLI signal patterns...
August 28, 2016: Biosensors & Bioelectronics
Joyce F Liu, Sangeetha Palakurthi, Qing Zeng, Shan Zhou, Elena Ivanova, Wei Huang, Ioannis K Zervantonakis, Laura M Selfors, Yiping Shen, Colin C Pritchard, Mei Zheng, Vilmos Adleff, Eniko Papp, Huiying Piao, Marian Novak, Susan Fotheringham, Gerburg Wulf, Jessie English, Paul T Kirschmeier, Victor E Velculescu, Cloud P Paweletz, Gordon B Mills, David M Livingston, Joan S Brugge, Ursula A Matulonis, Ronny Drapkin
PURPOSE: Ovarian cancer is the leading cause of death from gynecologic malignancy in the United States, with high rates of recurrence and eventual resistance to cytotoxic chemotherapy. Model systems that allow for accurate and reproducible target discovery and validation are needed to support further drug development in this disease. EXPERIMENTAL DESIGN: Clinically-annotated patient-derived xenograft (PDX) models were generated from tumor cells isolated from the ascites or pleural fluid of patients undergoing clinical procedures...
August 29, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Xiu Juan Li, Prakash Gangadaran, Senthilkumar Kalimuthu, Ji Min Oh, Liya Zhu, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Several clinical studies have demonstrated that increased macrophage infiltration into tumors confers metastatic potential and poor prognosis in cancer. Preclinical studies are needed to develop new strategies for countering metastasis. Our study was designed to investigate the impact of pulmonary macrophages on lung metastasis of anaplastic thyroid cancer (ATC). ATC (CAL-62) and macrophage (Raw264.7) were transfected with the effluc (CAL-62/effluc, Raw264.7/effluc). Coculture and migration assays were used to assess the effect of Raw264...
December 1, 2016: International Journal of Cancer. Journal International du Cancer
Jing Jiang, Lihou Dong, Lei Wang, Ling Wang, Jing Zhang, Fang Chen, Xiuli Zhang, Min Huang, Shenjun Li, Weiwei Ma, Qiaoyu Xu, Changjiang Huang, Jianmin Fang, Chunhua Wang
HER2 targeted delivery of ovarian cancer therapy has been beneficial for some patients, although, its efficacy is yet to be confirmed in large populations. We generated a novel anti-HER2 humanized antibody (Hertuzumab) and conjugated it to a microtubule-disrupting drug monomethyl auristatin E conjugate (MMAE) with a lysosomal protease-cleavable valine-citrulline linker. The average drug to antibody ratio (DAR) of Hertuzumab-vc-MMAE was varied by conjugating Hertuzumab antibodies with increasing linker-drugs (LDs) from D0-D8...
October 10, 2016: European Journal of Pharmaceutical Sciences
Wendy A Lea, Pierce T O'Neil, Alexandra J Machen, Subhashchandra Naik, Tapan Chaudhri, Wesley McGinn-Straub, Alexander Tischer, Matthew T Auton, Joshua R Burns, Michael R Baldwin, Karen R Khar, John Karanicolas, Mark T Fisher
Stabilizing the folded state of metastable and/or aggregation-prone proteins through exogenous ligand binding is an appealing strategy for decreasing disease pathologies caused by protein folding defects or deleterious kinetic transitions. Current methods of examining binding of a ligand to these marginally stable native states are limited because protein aggregation typically interferes with analysis. Here, we describe a rapid method for assessing the kinetic stability of folded proteins and monitoring the effects of ligand stabilization for both intrinsically stable proteins (monomers, oligomers, and multidomain proteins) and metastable proteins (e...
September 6, 2016: Biochemistry
Jeremie Cosette, Rym Ben Abdelwahed, Sabrina Donnou-Triffault, Catherine Sautès-Fridman, Patrice Flaud, Sylvain Fisson
Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. Here we describe a method for bioluminescence imaging to obtain a semi-quantitative analysis of tumor burden and treatment response. This method is based on the calculation of a luminoscore, a value that allows comparisons of two animals from the same or different experiments. Current BLI instruments enable the calculation of this luminoscore, which relies mainly on the acquisition conditions (back and front acquisitions) and the drawing of the region of interest (manual markup around the mouse)...
2016: Journal of Visualized Experiments: JoVE
Andrew T V Ho, Helen M Blau
Muscle stem cells play a central role in muscle regeneration. Most studies in the field of muscle regeneration focus on the unraveling of muscle stem cell biology to devise strategies for treating failing muscles as seen in aging and muscle-related diseases. However, the common method used in assessing stem cell function in vivo is laborious, as it involves time-consuming immunohistological analyses by microscopy on serial cryo-sections of the muscle post stem cell transplantation. Here we describe an alternative method, which adapts the bioluminescence imaging (BLI) technique to allow noninvasive tracking of engrafted stem-cell function in vivo in real-time...
2016: Methods in Molecular Biology
Dongzhi Yang, Liangzhu Feng, Casey A Dougherty, Kathryn E Luker, Daiqin Chen, Meagan A Cauble, Mark M Banaszak Holl, Gary D Luker, Brian D Ross, Zhuang Liu, Hao Hong
Angiogenesis, i.e. the formation of neovasculatures, is a critical process during cancer initiation, progression, and metastasis. Targeting of angiogenic markers on the tumor vasculature can result in more efficient delivery of nanomaterials into tumor since no extravasation is required. Herein we demonstrated efficient targeting of breast cancer metastasis in an experimental murine model with nano-graphene oxide (GO), which was conjugated to a monoclonal antibody (mAb) against follicle-stimulating hormone receptor (FSHR)...
October 2016: Biomaterials
Moussa Chehade, Amit K Srivastava, Jeff W M Bulte
We present a practical approach for co-registration of bioluminescence tomography (BLT), computed tomography (CT), and magnetic resonance (MR) images. To this end, we developed a customized animal shuttle composed of non-fluorescent, MR-compatible Delrin plastic that fits a commercially available MR surface coil. Mouse embryonic stem cells (mESCs) were transfected with the luciferase gene and labeled with superparamagnetic iron oxide (SPIO) nanoparticles. Cells were stereotaxically implanted in mouse brain and imaged weekly for 4 weeks with BLI (IVIS Spectrum CT scanner) and MRI (11...
June 2016: Tomography: a Journal for Imaging Research
Fabio Stellari, Gabriella Bergamini, Francesca Ruscitti, Angela Sandri, Francesca Ravanetti, Gaetano Donofrio, Federico Boschi, Gino Villetti, Claudio Sorio, Barouk M Assael, Paola Melotti, Maria M Lleo
BACKGROUND: Experimentally, lung inflammation in laboratory animals is usually detected by the presence of inflammatory markers, such as immune cells and cytokines, in the bronchoalveolar lavage fluid (BALF) of sacrificed animals. This method, although extensively used, is time, money and animal life consuming, especially when applied to genetically modified animals. Thus a new and more convenient approach, based on in vivo imaging analysis, has been set up to evaluate the inflammatory response in the lung of CFTR-deficient (CF) mice, a murine model of cystic fibrosis...
2016: Journal of Translational Medicine
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