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https://www.readbyqxmd.com/read/28620589/transcriptomic-profiling-of-high-density-giardia-foci-encysting-in-the-murine-proximal-intestine
#1
Jonathan K Pham, Christopher Nosala, Erica Y Scott, Kristofer F Nguyen, Kari D Hagen, Hannah N Starcevich, Scott C Dawson
Giardia is a highly prevalent, understudied protistan parasite causing significant diarrheal disease worldwide. Its life cycle consists of two stages: infectious cysts ingested from contaminated food or water sources, and motile trophozoites that colonize and attach to the gut epithelium, later encysting to form new cysts that are excreted into the environment. Current understanding of parasite physiology in the host is largely inferred from transcriptomic studies using Giardia grown axenically or in co-culture with mammalian cell lines...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28605477/mertk-as-a-therapeutic-target-in-glioblastoma
#2
Jing Wu, Lauren N Frady, Ryan E Bash, Stephanie M Cohen, Allison N Schorzman, Yu-Ting Su, David M Irvin, William C Zamboni, Xiaodong Wang, Stephen V Frye, Matthew G Ewend, Erik P Sulman, Mark R Gilbert, H Shelton Earp, C Ryan Miller
Background: Glioma-associated macrophages and microglia (GAMs) are components of the glioblastoma (GBM) microenvironment that express MerTK, a receptor tyrosine kinase that triggers efferocytosis and can suppress innate immune responses. The aim of the study was to define MerTK as a therapeutic target using an orally bioavailable inhibitor, UNC2025. Methods: We examined MerTK expression in tumor cells and macrophages in matched patient GBM samples by double-label immunohistochemistry...
June 12, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28599089/initial-graft-size-and-not-the-innate-immune-response-limit-survival-of-engrafted-neural-stem-cells
#3
Stefanie Vogel, Markus Aswendt, Melanie Nelles, Nadine Henn, Gabriele Schneider, Mathias Hoehn
Transplantation of neural stem cells (NSCs) appears as the most promising regenerative therapy for a variety of neurological disorders. Nevertheless, NSC engraftment is limited by the number of surviving cells. To maximize stem cell-mediated effects, timing of implantation and cell number have to be precisely evaluated. Here, we optimized a transgenic murine NSC line for high expression levels of the imaging reporters Luc2 and copGFP. NSCs of 150 000, 75 000, 15 000, or 1 500 cells or Hanks buffered salt solution were implanted into the striatum of nude mice...
June 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28596985/linked-color-imaging-improves-the-visibility-of-colorectal-polyps-a-video-study
#4
Naohisa Yoshida, Yuji Naito, Takaaki Murakami, Ryohei Hirose, Kiyoshi Ogiso, Yutaka Inada, Osamu Dohi, Kazuhiro Kamada, Kazuhiko Uchiyama, Osamu Handa, Hideyuki Konishi, Kewin Tien Ho Siah, Nobuaki Yagi, Yasuko Fujita, Mitsuo Kishimoto, Akio Yanagisawa, Yoshito Itoh
BACKGROUND/STUDY AIM:  Linked color imaging (LCI) by a laser endoscope (Fujifilm Co, Tokyo, Japan) is a novel narrow band light observation. In this study, we aimed to investigate whether LCI could improve the visibility of colorectal polyps using endoscopic videos. PATIENTS AND METHODS:  We prospectively recorded videos of consecutive polyps 2 - 20 mm in size diagnosed as neoplastic polyps. Three videos, white light (WL), blue laser imaging (BLI)-bright, and LCI, were recorded for each polyp by one expert...
June 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28596787/clinical-usefulness-of-the-vs-classification-system-using-magnifying-endoscopy-with-blue-laser-imaging-for-early-gastric-cancer
#5
Yoshikazu Yoshifuku, Yoji Sanomura, Shiro Oka, Kazutaka Kuroki, Mio Kurihara, Takeshi Mizumoto, Yuji Urabe, Toru Hiyama, Shinji Tanaka, Kazuaki Chayama
BACKGROUND: Blue laser imaging (BLI) enables the acquisition of more information from tumors' surfaces compared with white light imaging. Few reports confirm the validity of magnifying endoscopy (ME) with BLI (ME-BLI) for early gastric cancer (EGC). We aimed to assess the detailed endoscopic findings from EGCs using ME-BLI. METHODS: We enrolled 386 consecutive patients with 417 EGCs that were diagnosed using ME-BLI and resected by endoscopic submucosal dissection...
2017: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/28596158/molecular-imaging-of-human-embryonic-stem-cells-stably-expressing-human-pet-reporter-genes-after-zinc-finger-nucleases-mediated-genome-editing
#6
Esther Wolfs, Bryan Holvoet, Laura Ordovas, Natacha Breuls, Nicky Helsen, Matthias Schönberger, Susanna Raitano, Tom Struys, Bert Vanbilloen, Cindy Casteels, Maurilio Sampaolesi, Koen Van Laere, Ivo Lambrichts, Catherine M Verfaillie, Christophe M Deroose
Molecular imaging is indispensable for determining the fate and persistence of engrafted stem cells. Standard strategies for transgene induction involve the use of viral vectors prone to silencing and insertional mutagenesis or the use of non-human genes. We used zinc finger nucleases (ZFN) to induce stable expression of human imaging reporter genes into the safe harbor locus adeno-associated virus integration site 1 (AAVS1) in human embryonic stem cells (ESC). Plasmids were generated carrying reporter genes for fluorescence, bioluminescence imaging (BLI), and human positron emission tomography (PET) reporter genes...
June 8, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28591808/relationship-between-%C3%AE-lactamase-production-and-resistance-phenotype-in-klebsiella-pneumoniae-strains
#7
Jeongjin Kim, Tian Ding, Juhee Ahn
This study was designed to evaluate the resistance phenotypes of β-lactamases-producing Klebsiella pneumoniae strains. The antibiotic susceptibility and β-lactamase activity of K. pneumoniae strains, including antibiotic-sensitive K. pneumoniae (KPWT), ciprofloxacin-induced resistant K. pneumoniae (KPCIP), and clinically isolated K. pneumoniae strains (KPCI237, KPCI263, and KPCI272), were determined in the absence and presence of β-lactamase inhibitors (BLI 489, sulbactam, clavulanate, and tazobactam). All strains were highly resistant to ampicillin in the absence of β-lactamase inhibitors (MIC≥ 512 μg mL-1)...
June 7, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28558368/molecular-imaging-of-inducible-vegf-expression-and-tumor-progression-in-a-breast-cancer-model
#8
Lu Liang, Zhiwei Yue, Wei Du, Yang Li, Hongyan Tao, Di Wang, Ran Wang, Ziwei Huang, Ningning He, Xiaoyan Xie, Zhongchao Han, Na Liu, Zongjin Li
BACKGROUND: Tumor derived vascular endothelial growth factor (VEGF) can stimulate proliferation and migration of endothelial cells and recruit endothelial progenitor cells into tumors for vascular formation via a paracrine manner. Now increasing evidence suggests that VEGF also serves as an autocrine factor promoting cell survival and tumor angiogenesis. Real time visualization of VEGF activity in the early stages of tumor formation using molecular imaging will provide unprecedented insight into the biological processes of cancer...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28557444/complex-and-hierarchical-2d-assemblies-via-crystallization-driven-self-assembly-of-poly-l-lactide-homopolymers-with-charged-termini
#9
Xiaoming He, Yunxiang He, Ming-Siao Hsiao, Robert L Harniman, Samuel Pearce, Mitchell A Winnik, Ian Manners
Poly(L-lactide) (PLLA)-based nanoparticles have attracted much attention with respect to applications in drug delivery and nanomedicine as a result of their biocompatibility and biodegradability. Nevertheless, the ability to prepare PLLA assem-blies with well-defined shape and dimensions is limited and represents key challenge. Herein we report access to a series of monodisperse complex and hierarchical colloidally-stable 2D structures based on PLLA cores using the seeded growth, "liv-ing-crystallization-driven self-assembly" method...
May 30, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28538756/psychiatric-evaluation-of-civil-capacity-with-the-new-brazilian-statute-of-the-person-with-disabilities
#10
Elias Abdalla-Filho
The objective of this paper is to provide an update to psychiatrists regarding the new Brazilian Law for the Inclusion of People with Disabilities (BLI, Law 13,146 of 2015), and, specifically, to discuss potential implications of situations in which examination by a forensic psychiatrist points toward civil incompetence, while the above-mentioned law mandates full civil capacity for disabled persons. A study of Law 13,146/2015 was conducted, including a comparative analysis of legal and psychiatric approaches on the subject...
May 22, 2017: Revista Brasileira de Psiquiatria
https://www.readbyqxmd.com/read/28514082/universal-label-free-in-process-quantification-of-influenza-virus-like-particles
#11
Sofia B Carvalho, Mafalda G Moleirinho, David Wheatley, John Welsh, René Gantier, Paula M Alves, Cristina Peixoto, Manuel J T Carrondo
Virus-like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess. However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress. Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion. These analytical technologies are time-consuming, cumbersome, and not supportive of efficient downstream process development and monitoring...
May 17, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28501458/in-vivo-molecular-bioluminescence-imaging-new-tools-and-applications
#12
REVIEW
Laura Mezzanotte, Moniek van 't Root, Hacer Karatas, Elena A Goun, Clemens W G M Löwik
in vivo bioluminescence imaging (BLi) is an optical molecular imaging technique used to visualize molecular and cellular processes in health and diseases and to follow the fate of cells with high sensitivity using luciferase-based gene reporters. The high sensitivity of this technique arises from efficient photon production, followed by the reaction between luciferase enzymes and luciferin substrates. Novel discoveries and developments of luciferase reporters, substrates, and gene-editing techniques, and emerging fields of applications, promise a new era of deeper and more sensitive molecular imaging...
July 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28495463/development-of-an-animal-model-of-progressive-vaccinia-in-nu-nu-mice-and-the-use-of-bioluminescence-imaging-for-assessment-of-the-efficacy-of-monoclonal-antibodies-against-vaccinial-b5-and-l1-proteins
#13
Marina Zaitseva, Antonia Thomas, Clement A Meseda, Charles Y K Cheung, Claudia G Diaz, Yan Xiang, Shane Crotty, Hana Golding
Bioluminescence imaging (BLI) was used to follow dissemination of recombinant vaccinia virus (VACV) expressing luciferase (IHD-J-Luc) in BALB/c nu/nu mice treated post-challenge with monoclonal antibodies (MAbs) against L1 and B5 VACV proteins in a model of Progressive Vaccinia (PV). Areas Under the flux Curve (AUC) were calculated for viral loads in multiple organs in individual mice. Following scarification with 10(5) pfu, IHD-J-Luc VACV undergoes fast replication at the injection site and disseminates rapidly to the inguinal lymph nodes followed by spleen, liver, and axillary lymph nodes within 2-3 days and before primary lesions are visible at the site of scarification...
May 8, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28472196/-18f-fludarabine-pet-in-a-murine-model-of-multiple-myeloma
#14
Narinée Hovhannisyan, Martine Dhilly, Martin Fidalgo, Fabien Fillesoye, Stéphane Guillouet, Brigitte Sola, Louisa Barré
PURPOSE: Multiple myeloma (MM) is a haematological malignancy that affects plasma cells in the bone marrow. Recently, [18F]fludarabine has been introduced as an innovative PET radiotracer for imaging lymphoma. It demonstrated a great potential for accurate imaging of lymphoproliferative disorders. With the goal to question the usefulness of [18F]fludarabine-PET in other haematological diseases, an in vivo MM model was investigated. METHODS: RPMI8226-GFP-Luc MM cells expressing the green fluorescent protein (GFP) as well as the luciferase reporter (Luc) were derived from the parental RPMI8226 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28448040/determination-of-high-affinity-antibody-antigen-binding-kinetics-using-four-biosensor-platforms
#15
Danlin Yang, Ajit Singh, Helen Wu, Rachel Kroe-Barrett
Label-free optical biosensors are powerful tools in drug discovery for the characterization of biomolecular interactions. In this study, we describe the use of four routinely used biosensor platforms in our laboratory to evaluate the binding affinity and kinetics of ten high-affinity monoclonal antibodies (mAbs) against human proprotein convertase subtilisin kexin type 9 (PCSK9). While both Biacore T100 and ProteOn XPR36 are derived from the well-established Surface Plasmon Resonance (SPR) technology, the former has four flow cells connected by serial flow configuration, whereas the latter presents 36 reaction spots in parallel through an improvised 6 x 6 crisscross microfluidic channel configuration...
April 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28420436/autophagy-mediates-the-beneficial-effect-of-hypoxic-preconditioning-on-bone-marrow-mesenchymal-stem-cells-for-the-therapy-of-myocardial-infarction
#16
Zheng Zhang, Chao Yang, Mingzhi Shen, Ming Yang, Zhitao Jin, Liping Ding, Wei Jiang, Junke Yang, Haixu Chen, Feng Cao, Taohong Hu
BACKGROUND: Stem cell therapy has emerged as a promising therapeutic strategy for myocardial infarction (MI). However, the poor viability of transplanted stem cells hampers their therapeutic efficacy. Hypoxic preconditioning (HPC) can effectively promote the survival of stem cells. The aim of this study was to investigate whether HPC improved the functional survival of bone marrow mesenchymal stem cells (BM-MSCs) and increased their cardiac protective effect. METHODS: BM-MSCs, isolated from Tg(Fluc-egfp) mice which constitutively express both firefly luciferase (Fluc) and enhanced green fluorescent protein (eGFP), were preconditioned with HPC (1% O2) for 12 h, 24 h, 36 h, and 48 h, respectively, followed by 24 h of hypoxia and serum deprivation (H/SD) injury...
April 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28412561/metastatic-human-breast-cancer-to-the-spine-produces-mechanical-hyperalgesia-and-gait-deficits-in-rodents
#17
Rachel Sarabia-Estrada, Alejandro Ruiz-Valls, Hugo Guerrero-Cazares, Ana M Ampuero, Ismael Jimenez-Estrada, Samantha De Silva, Lydia J Bernhardt, C Rory Goodwin, A Karim Ahmed, Yuxin Li, Neil A Phillips, Ziya L Gokaslan, Alfredo Quiñones-Hinojosa, Daniel M Sciubba
BACKGROUND CONTEXT: Metastases to the spine are a common source of severe pain in cancer patients. The secondary effects of spinal metastases include pain, bone fractures, hypercalcemia, and neurological deficits. As the disease progresses, pain severity can increase until it becomes refractory to medical treatments and leads to a decreased quality of life for patients. A key obstacle in the study of pain-induced spinal cancer is the lack of reliable and reproducible spine cancer animal models...
April 13, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28408212/application-of-molecular-imaging-technology-in-evaluating-the-inhibiting-effect-of-apigenin-in%C3%A2-vivo-on-subcutaneous-hepatocellular-carcinoma
#18
Gang Li, Chong-Wei Chi, Xian-Fang Shao, Chi-Hua Fang
The aim of this study was to evaluate the inhibiting effect of apigenin on liver cancer in vivo based on the optical molecular imaging method. Subcutaneous liver tumor models were established using respective 1 × 10(6) firefly luciferase (fLuc) and green fluorescent protein (GFP) labeled human hepatocellular carcinoma cells (HepG2-fLuc and HepG2-GFP cells) in 20 BALB/c nude mice which were randomly divided into two groups, 10 in each group. After the tumor cells were implanted 15 days, apigenin was administered through intraperitoneal injection in group B, the other ten mice as control group A...
May 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28382163/visualization-of-a-neurotropic-flavivirus-infection-in-mouse-reveals-unique-viscerotropism-controlled-by-host-type-i-interferon-signaling
#19
Xiao-Feng Li, Xiao-Dan Li, Cheng-Lin Deng, Hao-Long Dong, Qiu-Yan Zhang, Qing Ye, Han-Qing Ye, Xing-Yao Huang, Yong-Qiang Deng, Bo Zhang, Cheng-Feng Qin
Flavivirus includes a large group of human pathogens with medical importance. Especially, neurotropic flaviviruses capable of invading central and peripheral nervous system, e.g. Japanese encephalitis virus (JEV) and Zika virus (ZIKV), are highly pathogenic to human and constitute major global health problems. However, the dynamic dissemination and pathogenesis of neurotropic flavivirus infections remain largely unknown. Here, using JEV as a model, we rationally designed and constructed a recombinant reporter virus that stably expressed Renilla luciferase (Rluc)...
2017: Theranostics
https://www.readbyqxmd.com/read/28378292/tracking-dendritic-cell-migration-into-lymph-nodes-by-using-a-novel-pet-probe-18-f-tetrafluoroborate-for-sodium-iodide-symporter
#20
Sang Bong Lee, Ho Won Lee, Hongje Lee, Yong Hyun Jeon, Sang-Woo Lee, Byeong-Cheol Ahn, Jaetae Lee, Shin Young Jeong
BACKGROUND: Recently, (18)F-tetrafluoroborate (TFB) was used as a substrate for the human sodium/iodide symporter (hNIS) reporter gene. This study evaluated the feasibility of performing molecular-genetic imaging by using the new radiotracer ((18)F-TFB) for the hNIS gene, to track dendritic cell (DC) migration in live mice. A murine dendritic cell line (DC2.4) co-expressing the hNIS and effluc genes (DC/NF) was established. To confirm the functional cellular expression of both effluc and NIS in the inoculated DC/NF cells by bio-medical imaging, combined bioluminescence imaging (BLI) and (18)F-TFB positron emission tomography/computed tomography (PET/CT) imaging was performed after intramuscular injection with parental DCs and DC/NF cells...
December 2017: EJNMMI Research
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