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https://www.readbyqxmd.com/read/27902381/detection-of-escherichia-coli-st131-complex-st705-and-klebsiella-pneumoniae-st15-among-fecal-carriage-of-extended-spectrum-betalactamase-and-carbapenemase-producing-enterobacteriaceae
#1
Esther Rios, Maria Carmen López, Iciar Rodríguez-Avial, Esther Culebras, Juan Jose Picazo
The objective of the present study was to evaluate the prevalence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and carbapenemases-producing Enterobacteriaceae (CPE) in non-selected hospitalized and non-hospitalized patients from the same geographic area of Madrid. Among the 501 patients enrolled, 43 (8.6%) carried ESBL-E and 8 (1.6%) patients exhibited CPE. The main intestinal colonizer among ESBL-E was CTX-M-producing Escherichia coli isolates in both settings (community and hospital)...
November 28, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27897435/-evaluation-of-an-immunochromatographic-test-for-the-detection-of-oxa-48-carbapenemase
#2
C Mediavilla-Gradolph, R Sáinz-Rodriguez, M Valverde-Troya, I de Toro-Peinado, M P Bermudez-Ruíz, B Palop-Borrás
OBJECTIVE: Detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. OXA-48 is the most common carbapenemase in Spain and in our environment. The aim of this study is the evaluation of a new immunochromatographic test OXA-48 Card letitest (Coris, BioConcept Belgium) to detect this carbapenemase from solid media. METHODS: During the last year 151 strains of carbapenemase producing bacteria have been isolated, of which 136 were OXA-48 (126 Klebsiella pneumoniae, 1 Klebsiella oxytoca, 5 Escherichia coli, 4 Enterobacter cloacae), and 15 producing other carbapenemases ...
November 25, 2016: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/27897299/diastereoselective-synthesis-of-trifluoromethylated-1-3-dioxanes-by-intramolecular-oxa-michael-reaction
#3
Liliana Becerra-Figueroa, Elodie Brun, Michael Mathieson, Louis J Farrugia, Claire Wilson, Joëlle Prunet, Diego Gamba-Sánchez
A highly diastereoselective synthesis of trifluoromethylated 1,3-dioxanes is described. The reaction proceeds by an addition/oxa-Michael sequence and works efficiently under mild reaction conditions, with a good substrate scope and acceptable to good yields.
November 29, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27895627/distribution-and-classification-of-serine-%C3%AE-lactamases-in-brazilian-hospital-sewage-and-other-environmental-metagenomes-deposited-in-public-databases
#4
Adriana M Fróes, Fábio F da Mota, Rafael R C Cuadrat, Alberto M R Dávila
β-lactam is the most used antibiotic class in the clinical area and it acts on blocking the bacteria cell wall synthesis, causing cell death. However, some bacteria have evolved resistance to these antibiotics mainly due the production of enzymes known as β-lactamases. Hospital sewage is an important source of dispersion of multidrug-resistant bacteria in rivers and oceans. In this work, we used next-generation DNA sequencing to explore the diversity and dissemination of serine β-lactamases in two hospital sewage from Rio de Janeiro, Brazil (South Zone, SZ and North Zone, NZ), presenting different profiles, and to compare them with public environmental data available...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27890667/investigation-of-carbapenem-resistance-and-the-first-identification-of-klebsiella-pneumoniae-carbapenemase-kpc-enzyme-among-escherichia-coli-isolates-in-turkey-a-prospective-study
#5
Mert Ahmet Kuskucu, Asiye Karakullukcu, Mailihaba Ailiken, Barıs Otlu, Bilgul Mete, Gokhan Aygun
BACKGROUND: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. METHODS: The prospective study included a total of 4052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively...
November 23, 2016: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/27890443/oxa-23-and-isaba1-oxa-66-class-d-%C3%AE-lactamases-in-acinetobacter-baumannii-isolates-from-companion-animals
#6
Christa Ewers, Peter Klotz, Ursula Leidner, Ivonne Stamm, Ellen Prenger-Berninghoff, Stephan Göttig, Torsten Semmler, Sandra Scheufen
Acinetobacter baumannii is recognised as a major pathogen of nosocomial infections that frequently show resistance to last-resort antimicrobials. To investigate whether A. baumannii from companion animals harbour carbapenem resistance mechanisms, 223 clinical isolates obtained from veterinary clinics between 2000 and 2013 in Germany were screened for carbapenem-non-susceptibility employing meropenem-containing Mueller-Hinton agar plates. Minimum inhibitory concentration (MIC) data were obtained using the VITEK(®)2 system...
November 16, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27887844/anthraquinones-from-morinda-longissima-and-their-insulin-mimetic-activities-via-amp-activated-protein-kinase-ampk-activation
#7
Phi-Hung Nguyen, Hong Seok Choi, Thi Kim Quy Ha, Ji Yeon Seo, Jun-Li Yang, Da-Woon Jung, Darren R Williams, Won-Keun Oh
AMP-activated protein kinase (AMPK) activators are known to increase energy metabolism and to reduce body weight, as well as to improve glucose uptake. During for searching AMPK activators, a new anthraquinone, modasima A (10), along with eighteen known analogues (1-9 and 11-19) were isolated from an ethanol extract of the roots of Morinda longissima Y. Z. Ruan (Rubiaceae). Using the fluorescent tagged glucose analogues, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-D-glucose (2-NBDG), insulin mimetics were screened with compounds 1-19 in 3T3-L1 adipocytes...
November 15, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27881972/multilocus-sequence-typing-analysis-of-carbapenem-resistant-acinetobacter-baumannii-in-a-chinese-burns-institute
#8
Guangtao Huang, Supeng Yin, Yali Gong, Xia Zhao, Lingyun Zou, Bei Jiang, Zhiwei Dong, Yu Chen, Jing Chen, Shouguang Jin, Zhiqiang Yuan, Yizhi Peng
Acinetobacter baumannii is a leading pathogen responsible for nosocomial infections. The emergence of carbapenem-resistant A. baumannii (CRAB) has left few effective antibiotics for clinicians to use. To investigate the temporal evolutionary relationships among CRAB strains, we collected 248 CRAB isolates from a Chinese burns institute over 3 years. The prevalence of the OXA-23 gene was detected by polymerase chain reaction. Multilocus sequence typing was used to type the CRAB strains and eBURST was used to analyze their evolutionary relationships...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27876317/discovery-of-soat2-inhibitors-from-synthetic-small-molecules
#9
Keisuke Kobayashi, Taichi Ohshiro, Hiroshi Tomoda, Feng Yin, Hai-Lei Cui, Pandurang V Chouthaiwale, Fujie Tanaka
Synthesis of new functionalized molecules and identification of biofunctional molecules can lead to the development of therapeutic leads and molecular tools for biomedical research. We have recently reported oxa-hetero-Diels-Alder reactions of enones with isatins to provide functionalized spirooxindole tetrahydropyran derivatives. Twenty-one compounds from the spirooxindole tetrahydropyran derivatives and related molecules were screened for inhibition of sterol O-acyltransferase (SOAT) isozymes SOAT1 and SOAT2...
December 15, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27866944/occurrence-of-carbapenemase-producing-klebsiella-pneumoniae-and-escherichia-coli-in-the-european-survey-of-carbapenemase-producing-enterobacteriaceae-euscape-a-prospective-multinational-study
#10
Hajo Grundmann, Corinna Glasner, Barbara Albiger, David M Aanensen, Chris T Tomlinson, Arjana Tambić Andrasević, Rafael Cantón, Yehuda Carmeli, Alexander W Friedrich, Christian G Giske, Youri Glupczynski, Marek Gniadkowski, David M Livermore, Patrice Nordmann, Laurent Poirel, Gian M Rossolini, Harald Seifert, Alkiviadis Vatopoulos, Timothy Walsh, Neil Woodford, Dominique L Monnet
BACKGROUND: Gaps in the diagnostic capacity and heterogeneity of national surveillance and reporting standards in Europe make it difficult to contain carbapenemase-producing Enterobacteriaceae. We report the development of a consistent sampling framework and the results of the first structured survey on the occurrence of carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in European hospitals. METHODS: National expert laboratories recruited hospitals with diagnostic capacities, who collected the first ten carbapenem non-susceptible clinical isolates of K pneumoniae or E coli and ten susceptible same-species comparator isolates and pertinent patient and hospital information...
November 17, 2016: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/27866753/-molecular-epidemiology-of-carbapenemase-producing-enterobacteriaceae-infection-colonisation-in-a-hospital-in-madrid
#11
Patricia Brañas, Marta Gil, Jennifer Villa, María Ángeles Orellana, Fernando Chaves
INTRODUCTION: A description is presented on the molecular epidemiology of carbapenemase-producing enterobacteriaceae infection in a tertiary hospital. MATERIAL AND METHODS: A study was made on all the carbapenemase-producing enterobacteriaceae isolations obtained between February 2015 and March 2016 in the Hospital Universitario 12 de Octubre (Madrid). Phenotypic and molecular methods were used. RESULTS: A total of 7 bacterial species were identified, with the majority being Klebsiella pneumoniae (K...
November 17, 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/27863949/imipenem-avibactam-a-novel-combination-for-the-rapid-detection-of-carbapenemase-activity-in-enterobacteriaceae-and-acinetobacter-baumannii-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#12
Marina Oviaño, Germán Bou
In the present study, we propose a novel matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for detecting carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii. For this, we analyzed a series of 131 isolates. Among them, a total of 115 Enterobacteriaceae: 79 of them carrying a carbapenemase enzyme (15blaKPC, 7blaNDM, 11blaIMP, 12blaVIM, and 34blaOXA-48) and 16 A. baumannii isolates: 15 of them carrying carbapenemases (10blaOXA-23, 2blaOXA-58, 2blaOXA-24, and 1blaOXA-237)...
October 18, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27855080/high-frequency-of-oxa-253-producing-acinetobacter-baumannii-in-different-hospitals-in-recife-brazil-a-new-threat
#13
Felipe Lira de Sá Cavalcanti, Carina Lucena Mendes-Marques, Crhisllane Rafaele Dos Santos Vasconcelos, Túlio de Lima Campos, Antonio Mauro Rezende, Danilo Elias Xavier, Nilma Cintra Leal, Osvaldo Pompilio de-Melo-Neto, Marcia Maria Camargo de Morais, Tereza Cristina Leal-Balbino
Here we report the isolation of 31 Acinetobacter baumannii strains producing OXA-253 in a single, large, Brazilian city. These strains belonged to five different STs, including four not previously associated to blaOXA-253 In all strains the blaOXA-253 gene was plasmid located but within seven different genetic environments. The reported data emphasizes the successful transmission of the blaOXA-253 through a large area and the tendency for this resistance determinant to remain in the A. baumannii population.
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27855079/chromosomal-amplification-of-the-blaoxa-58-carbapenemase-gene-in-a-proteus-mirabilis-clinical-isolate
#14
Delphine Girlich, Rémy A Bonnin, Pierre Bogaerts, Morgane De Laveleye, Daniel T Huang, Laurent Dortet, Philippe Glaser, Youri Glupczynski, Thierry Naas
Horizontal gene transfer may occur between distantly related bacteria thus leading to genetic plasticity and in some cases to acquisition of novel resistance traits. Proteus mirabilis is an Enterobacterial species responsible for human infections that may express various acquired ß-lactam resistance genes, including different classes of carbapenemase genes. Here we report a Proteus mirabilis clinical isolate (1091) displaying resistance to penicillin, including temocillin, together with reduced susceptibility to carbapenems and susceptibility to expanded-spectrum cephalosporins...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27855076/molecular-characterization-of-oxa-48-like-producing-enterobacteriaceae-in-the-czech-republic-evidence-for-horizontal-transfer-of-poxa-48-like-plasmids
#15
Anna Skálová, Kateřina Chudějová, Veronika Rotová, Matej Medvecky, Vendula Študentová, Eva Chudáčková, Pavel Lavicka, Tamara Bergerova, Vladislav Jakubu, Helena Zemlickova, Costas C Papagiannitsis, Jaroslav Hrabák
The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings, in the Czech Republic. During 2013-2015, twenty-two Klebsiella pneumoniae, three Escherichia coli and one Enterobacter cloacae producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized/infected by two or three different OXA-48-like producers. K. pneumoniae isolates were classified into nine STs, with ST101 being the predominant (n=8)...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27854165/antimicrobial-resistance-mechanisms-and-genetic-diversity-of-multidrug-resistant-acinetobacter-baumannii-isolated-from-a-teaching-hospital-in-malaysia
#16
Shirin Biglari, Alfizah Hanafiah, Shaliawani Mohd Puzi, Ramliza Ramli, Mostafizur Rahman, Bruno Silvester Lopes
Multidrug-resistant (MDR) Acinetobacter baumannii has increasingly emerged as an important nosocomial pathogen. The aim of this study was to determine the resistance profiles and genetic diversity in A. baumannii clinical isolates in a tertiary medical center in Malaysia. The minimum inhibitory concentrations of carbapenems (imipenem and meropenem), cephalosporins (ceftazidime and cefepime), and ciprofloxacin were determined by E-test. PCR and sequencing were carried out for the detection of antibiotic resistance genes and mutations...
November 17, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27854148/first-detection-of-ges-5-carbapenemase-producing-acinetobacter-baumannii-isolate
#17
Mohamed H Al-Agamy, Katy Jeannot, Taghrid S El-Mahdy, Atef M Shibl, Wael Kattan, Patrick Plésiat, Patrice Courvalin
This study was conducted to investigate the molecular epidemiology of resistance in Acinetobacter baumannii isolates collected at a hospital in Riyadh, Saudi Arabia, from January through December 2010. Twenty-seven A. baumannii were highly resistant (MIC90 > 256 μg/ml) to ceftazidime, cefepime, and aztreonam. Imipenem resistance was seen in 24 isolates, of which 18 had an minimum inhibitory concentration (MIC) >32 μg/mL. Ciprofloxacin, gentamicin, and amikacin resistance was found in 93%, 52%, and 37% of all the isolates, respectively...
November 17, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27853806/diastereoselective-synthesis-of-3-acetoxy-4-3-aryloxiran-2-yl-azetidin-2-ones-and-their-transformation-into-3-4-oxolane-fused-bicyclic-%C3%AE-lactams
#18
Nicola Piens, Sven De Craene, Jorick Franceus, Karen Mollet, Kristof Van Hecke, Tom Desmet, Matthias D'hooghe
cis-3-Acetoxy-4-(3-aryloxiran-2-yl)azetidin-2-ones were prepared through a Staudinger [2+2]-cyclocondensation between acetoxyketene and the appropriate epoxyimines in a highly diastereoselective way. Subsequent potassium carbonate-mediated acetate hydrolysis, followed by intramolecular ring closure through epoxide ring opening, afforded stereodefined 3-aryl-4-hydroxy-2-oxa-6-azabicyclo[3.2.0]heptan-7-ones as a novel class of C-fused bicyclic β-lactams. Selective benzylic oxidation of bicyclic N-(4-methoxybenzyl)-β-lactams with potassium persulfate and potassium dihydrogen phosphate provided the corresponding N-aroyl derivatives as interesting leads for further β-lactamase inhibitor development...
November 17, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27842873/in-vitro-activities-of-colistin-tigecycline-and-tobramycin-alone-or-in-combination-against-carbapenem-resistant-enterobacteriaceae-strains
#19
Berna Ozbek, Emel Mataracı-Kara, Sevda Er, Melda Ozdamar, Mesut Yilmaz
The aim of this study was to investigate the activities of various antibiotics, alone or in combination, against carbapenem-resistant Enterobacteriaceae (CRE). Minimum inhibitory concentrations (MICs) of meropenem, colistin, tigecycline and tobramycin were determined by microbroth dilution against 40 clinical strains. Carbapenemase-encoding genes were detected by PCR using specific primers. The in vitro synergistic activities of tigecycline, colistin and tobramycin in double antibiotic combinations were determined by the microbroth chequerboard technique, and results were interpreted using the fractional inhibitory concentration index (FICI)...
December 2015: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27834373/in-vivo-protein-interaction-network-analysis-reveals-porin-localized-antibiotic-inactivation-in-acinetobacter-baumannii-strain-ab5075
#20
Xia Wu, Juan D Chavez, Devin K Schweppe, Chunxiang Zheng, Chad R Weisbrod, Jimmy K Eng, Ananya Murali, Samuel A Lee, Elizabeth Ramage, Larry A Gallagher, Hemantha D Kulasekara, Mauna E Edrozo, Cassandra N Kamischke, Mitchell J Brittnacher, Samuel I Miller, Pradeep K Singh, Colin Manoil, James E Bruce
The nosocomial pathogen Acinetobacter baumannii is a frequent cause of hospital-acquired infections worldwide and is a challenge for treatment due to its evolved resistance to antibiotics, including carbapenems. Here, to gain insight on A. baumannii antibiotic resistance mechanisms, we analyse the protein interaction network of a multidrug-resistant A. baumannii clinical strain (AB5075). Using in vivo chemical cross-linking and mass spectrometry, we identify 2,068 non-redundant cross-linked peptide pairs containing 245 intra- and 398 inter-molecular interactions...
November 11, 2016: Nature Communications
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