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umbilical cord stem cells

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https://www.readbyqxmd.com/read/29460716/role-of-wild-type-and-recombinant-human-t-cell-leukemia-viruses-in-lymphoproliferative-disease-in-humanized-nsg-mice
#1
Devra D Huey, Brad Bolon, Krista M D La Perle, Priya Kannian, Steven Jacobson, Lee Ratner, Patrick L Green, Stefan Niewiesk
Chronic infection with human T-cell leukemia virus type 1 (HTLV1) can lead to adult T-cell leukemia (ATL). In contrast, infection with HTLV2 does not lead to leukemia, potentially because of distinct virus-host interactions and an active immune response that controls virus replication and, therefore, leukemia development. We created a humanized mouse model by injecting human umbilical-cord stem cells into the livers of immunodeficient neonatal NSG mice, resulting in the development of human lymphocytes that cannot mount an adaptive immune response...
February 1, 2018: Comparative Medicine
https://www.readbyqxmd.com/read/29457930/nano-loaded-human-umbilical-cord-mesenchymal-stem-cells-as-targeted-carriers-of-doxorubicin-for-breast-cancer-therapy
#2
Shunxiu Cao, Jiamin Guo, Yujing He, Murad Alahdal, Shanshan Tang, Yuekui Zhao, Zhaocong Yang, Huashan Gao, Wenjun Hu, Hulin Jiang, Lianju Qin, Liang Jin
The main challenge of anticancer drugs is poor tumor targeting. Now cellular carriers are widely investigated to deliver anticancer agents. As an ideal cellular candidate, human umbilical cord derived mesenchymal stem cells (hUC-MSCs) possess inherent tropism potential to tumor. Here, we constructed hUC-MSCs carrying transferrin-inspired-nanoparticles that contain doxorubicin(hUC-MSCs-Tf-inspired-NPs) to achieve enhanced anti-tumor efficacy and made an evaluation. Results represented that hUC-MSCs-Tf-inspired-NPs not only exhibit the controlled-release property of Tf-inspired-NPs, but also integrate tumor tropism and penetrative abilities of MSCs...
February 19, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29453764/comparative-analysis-of-human-ucb-and-adipose-tissue-derived-mesenchymal-stem-cells-for-their-differentiation-potential-into-brown-and-white-adipocytes
#3
Afrooz Rashnonejad, Gulinnaz Ercan, Cumhur Gunduz, Ali Akdemir, Yigit Ozer Tiftikcioglu
The differentiation potential of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) into brown and white adipocytes in comparison to Adipose tissue derived MSCs (AD-MSCs) were investigated in order to characterize their potency for future cell therapies. MSCs were isolated from ten UCB samples and six liposuction materials. MSCs were differentiated into white and brown adipocytes after characterization by flow cytometry. Differentiated adipocytes were stained with Oil Red O and hematoxylin/eosin...
February 16, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29453290/mouse-and-human-hspc-immobilization-in-liquid-culture-by-cd43-or-cd44-antibody-coating
#4
Dirk Loeffler, Weijia Wang, Alois Hopf, Oliver Hilsenbeck, Paul E Bourgine, Fabian Rudolf, Ivan Martin, Timm Schroeder
Keeping track of individual cell identifications is imperative to the study of dynamic single cell behavior over time. Highly motile hematopoietic stem and progenitor cells (HSPCs) migrate quickly and do not adhere, and thus must be imaged very frequently to keep cell identifications. Even worse, they are also flushed away during medium exchange. To overcome these limitations, we tested antibody coating for reducing HSPC motility in vitro. Anti-CD43 and -CD44 antibody coating reduced cell motility of mouse and human HSPCs in a concentration dependent manner...
February 16, 2018: Blood
https://www.readbyqxmd.com/read/29443032/kinetic-analysis-of-vasculogenesis-quantifies-dynamics-of-vasculogenesis-and-angiogenesis-in-vitro
#5
Kaela M Varberg, Seth Winfree, Kenneth W Dunn, Laura S Haneline
Vasculogenesis is a complex process by which endothelial stem and progenitor cells undergo de novo vessel formation. Quantitative assessment of vasculogenesis has become a central readout of endothelial progenitor cell functionality, and therefore, several attempts have been made to improve both in vitro and in vivo vasculogenesis models. However, standard methods are limited in scope, with static measurements failing to capture many aspects of this highly dynamic process. Therefore, the goal of developing this novel protocol was to assess the kinetics of in vitro vasculogenesis in order to quantitate rates of network formation and stabilization, as well as provide insight into potential mechanisms underlying vascular dysfunction...
January 31, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29442894/graphene-oxide-incorporated-plga-nanofibrous-scaffold-for-solid-phase-gene-delivery-into-mesenchymal-stem-cells
#6
Zhiying Wang, He Shen, Saijie Song, Liming Zhang, Wei Chen, Jianwu Dai, Zhijun Zhang
Delivery of functional genes into stem cells shows great application prospect in DNA-based tissue engineering. However, comparing with epithelial cells and cancer cells, stem cells usually exhibit low gene transfection efficiency. To enhance the transfection efficiency, non-viral gene delivery in combination with biomaterial scaffolds, has raised increasing interests from researchers in tissue engineering. Nanofibers fabricated by electrospinning technique mimicking extracellular matrix (ECM) are widely used in tissue engineering applications...
April 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29440630/human-umbilical-cord-mesenchymal-stem-cell-derived-extracellular-vesicles-promote-lung-adenocarcinoma-growth-by-transferring-mir-410
#7
Liyang Dong, Yanan Pu, Lina Zhang, Qianqian Qi, Lei Xu, Wei Li, Chuan Wei, Xiaofan Wang, Sha Zhou, Jifeng Zhu, Xuefeng Wang, Feng Liu, Xiaojun Chen, Chuan Su
Although accumulating evidence has linked mesenchymal stem cells (MSCs) with tumor growth, the underlying mechanisms are poorly understood. Here, we demonstrated for the first time that human umbilical cord MSCs (hUCMSCs) dramatically increased the growth of lung adenocarcinoma (LUAD) cancer cells in a xenograft tumor model. Then, we observed that hUCMSC-derived extracellular vesicles (hUCMSC-EVs) contribute to the hUCMSC-promoted LUAD cell growth through a direct effect on LUAD cells. Furthermore, we showed that hUCMSC-EV-mediated LUAD growth is associated with increased proliferation and decreased apoptosis in LUAD cells, concomitant with reduced PTEN expression mediated by the hUCMSC-EV-transmitted miR-410...
February 13, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29439436/chondrogenic-differentiation-of-defined-equine-mesenchymal-stem-cells-derived-from-umbilical-cord-blood-for-use-in-cartilage-repair-therapy
#8
Mélanie Desancé, Romain Contentin, Lélia Bertoni, Tangni Gomez-Leduc, Thomas Branly, Sandrine Jacquet, Jean-Marc Betsch, Agnès Batho, Florence Legendre, Fabrice Audigié, Philippe Galéra, Magali Demoor
Cartilage engineering is a new strategy for the treatment of cartilage damage due to osteoarthritis or trauma in humans. Racehorses are exposed to the same type of cartilage damage and the anatomical, cellular, and biochemical properties of their cartilage are comparable to those of human cartilage, making the horse an excellent model for the development of cartilage engineering. Human mesenchymal stem cells (MSCs) differentiated into chondrocytes with chondrogenic factors in a biomaterial appears to be a promising therapeutic approach for direct implantation and cartilage repair...
February 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29433563/therapeutic-effect-of-human-umbilical-cord-derived-mesenchymal-stem-cells-on-injured-rat-endometrium-during-its-chronic-phase
#9
Lu Zhang, Ying Li, Chun-Yi Guan, Shi Tian, Xiao-Dan Lv, Jian-Hui Li, Xu Ma, Hong-Fei Xia
BACKGROUND: Repair deficiency after endometrial injury is an important reason for intra-uterine adhesions, amenorrhea, and infertility in females. Bone marrow-derived mesenchymal stem cell (BMSC) transplantation is effective in repairing the damaged endometrium. However, the possibility of using umbilical cord-derived MSCs (UC-MSCs) to treat endometrial injury is rarely reported. METHODS: Ethanol (95%) was injected into rat uterus to establish a model of endometrial injury...
February 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29432583/the-clinical-utility-of-pgd-with-hla-matching-a-collaborative-multi-centre-eshre-study
#10
G Kakourou, S Kahraman, G C Ekmekci, H A Tac, G Kourlaba, E Kourkouni, A Cervero Sanz, J Martin, H Malmgren, C Giménez, V Gold, F Carvalho, C Billi, J F C Chow, X Vendrell, G Kokkali, J Liss, J Steffann, J Traeger-Synodinos
STUDY QUESTION: Has PGD-HLA been successful relative to diagnostic and clinical efficacy? SUMMARY ANSWER: The diagnostic efficacy of PGD-HLA protocols was found lower in this study in comparison to published PGD-HLA protocols and to that reported for general PGD by ESHRE (78.5 vs 94.1% and vs 92.6%, respectively), while the clinical efficacy has proven very difficult to assess due to inadequate follow-up of both the ART/PGD and HSCT procedure outcomes. WHAT IS KNOWN ALREADY: The first clinical cases for PGD-HLA were reported in 2001...
February 8, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29429941/quantitative-analysis-of-composite-umbilical-cord-tissue-health-using-a-standardized-explant-approach-and-an-assay-of-metabolic-activity
#11
Matthew L Skiles, Katherine S Brown, William Tatz, Kristen Swingle, Heather L Brown
BACKGROUND: Umbilical cord (UC) tissue can be collected in a noninvasive procedure and is enriched in progenitor cells with potential therapeutic value. Mesenchymal stromal cells (MSCs) can be reliably harvested from fresh or cryopreserved UC tissue by explant outgrowth with no apparent impact on functionality. A number of stem cell banks offer cryopreservation of UC tissue, alongside cord blood, for future cell-based applications. In this setting, measuring and monitoring UC quality is critical...
February 8, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29429512/effect-of-human-umbilical-cord-blood-derived-cd34-hematopoietic-stem-cell-on-the-expression-of-wnt4-and-p53-genes-in-a-rat-model-of-hepatocellular-carcinoma
#12
Rania Naiem Sherif, Hussein Abdellatif, Noha Hazem, Neven A Ebrahim, Dalia Saleh, Gamal Shiha, Huda Eltahry, Kamal G Botros, Omar M Gabr
BACKGROUND AND AIM OF THE WORK: Hepatocellular carcinoma (HCC) is the most frequent primary liver malignancy. Chronic liver injuries as chronic hepatitis C and hepatitis B viruses, aflatoxins consumption and nonalcoholic fatty liver disease are well-established causes of HCC. HCC is associated with a series of molecular changes, as alternation in glypican-3, P53 expression and Wnt/β-catenin pathway. Hepatic cancer progenitor cells could contribute to HCC development. This research aimed to study the effectiveness of human CD34+ hematopoietic stem cell on Wnt4 and P53 genes expression, histopathological grading and hepatic progenitor cells percentage in HCC rat model...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29426829/impaired-thymopoiesis-predicts-for-a-high-risk-of-severe-infections-after-reduced-intensity-conditioning-without-anti-thymocyte-globulin-in-double-umbilical-cord-blood-transplantation
#13
Lucia E Duinhouwer, Nick Beije, Bronno van der Holt, Anita Rijken-Schelen, Cor H Lamers, Judith Somers, Eric Braakman, Jan J Cornelissen
Umbilical cord blood stem cell transplantation (UCBT) is associated with retarded hematopoietic recovery and immune reconstitution and a high infection-related morbidity and mortality, especially after conditioning including anti-thymocyte globulin (ATG). However, data on immune recovery, incidence of infections, and outcome in double UCBT (dUCBT) recipients receiving an ATG-free reduced intensity conditioning (RIC) are lacking. In this study, recovery of lymphocyte subsets, thymopoiesis, and its association with severe infections and clinical outcome was assessed in a group of 55 recipients of a dUCBT ATG-free RIC regimen...
February 9, 2018: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/29423006/umbilical-cord-mesenchymal-stem-cell-transplantation-in-the-treatment-of-multiple-sclerosis
#14
Mingyao Meng, Ying Liu, Wenju Wang, Chuanyu Wei, Feifei Liu, Zhiqin Du, Yanhua Xie, Weiwei Tang, Zongliu Hou, Qihan Li
To investigate the clinical efficacy and safety of umbilical cord mesenchymal stem cell (UCMSC) transplantation for treating multiple sclerosis (MS), the patients with MS were recruited and treated with UCMSC. The procedure of preparing UCMSC was in accordance with the standards formulated by the International Society for Cell Biology. Cell surface markers, multiple differentiation potential and safety of UCMSC for transplantation were detected. The number of cells in each infusion was 1 to 2×10 6 cells/kg...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29422997/hucmsc-exosomes-delivered-14-3-3%C3%AE-enhanced-autophagy-via-modulation-of-atg16l-in-preventing-cisplatin-induced-acute-kidney-injury
#15
Haoyuan Jia, Wanzhu Liu, Bin Zhang, Juanjuan Wang, Peipei Wu, Nitin Tandra, Zhaofeng Liang, Cheng Ji, Lei Yin, Xinyuan Hu, Yongmin Yan, Fei Mao, Xu Zhang, Jing Yu, Wenrong Xu, Hui Qian
The clinical application of cisplatin is restricted by its side effects of nephrotoxicity. Human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-ex) have an important effect in tissue injury repair. Our previous work discovered that pretreatment with human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-ex) alleviated cisplatin-induced acute kidney injury (AKI) by activating autophagy both in vitro and in vivo. In this study, we further explored the mechanisms of hucMSC-ex in autophagy for preventing cisplatin-induced nephrotoxicity...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29422210/pathophysiology-of-placental-derived-fetal-growth-restriction
#16
REVIEW
Graham J Burton, Eric Jauniaux
Placental-related fetal growth restriction arises primarily due to deficient remodeling of the uterine spiral arteries supplying the placenta during early pregnancy. The resultant malperfusion induces cell stress within the placental tissues, leading to selective suppression of protein synthesis and reduced cell proliferation. These effects are compounded in more severe cases by increased infarction and fibrin deposition. Consequently, there is a reduction in villous volume and surface area for maternal-fetal exchange...
February 2018: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29421958/treating-childhood-traumatic-brain-injury-with-autologous-stem-cell-therapy
#17
Shyam Dewan, Samantha Schimmel, Cesar V Borlongan
Neonatal traumatic brain injury (TBI) is a significant cause of developmental disorders. Autologous stem cell therapy may enhance neonatal brain plasticity towards repair of the injured neonatal brain. Areas Covered: The endogenous neonatal anti-inflammatory response can be enhanced by biological treatments. Stem cell therapy stands as a robust approach for sequestering the inflammation-induced cell death in the injured brain. Here, we discuss the use of umbilical cord blood cells and bone marrow stromal cells for acute and chronic treatment of experimental neonatal TBI...
February 9, 2018: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/29417230/identification-of-megakaryocytes-as-a-target-of-advanced-glycation-end-products-in-diabetic-complications-in-bone-marrow
#18
Benfang Wang, Jianjiang Yu, Ting Wang, Ying Shen, Dandan Lin, Xin Xu, Yiqiang Wang
AIMS: To define the possible effect of diabetic conditions on megakaryocytes, the long-know precursors of platelets and lately characterized modulator of hematopoietic stem quiescence-activation transition. METHODS: Megakaryoblastic MEG-01 cell culture and TPO/SCF/IL-3-induced differentiation of human umbilical blood mononuclear cells toward megakaryocytes were used to test effects of glycated bovine serum albumin (BSA-AGEs). The ob/ob mice and streptozotocin-treated mice were used as models of hyperglycemia...
February 8, 2018: Acta Diabetologica
https://www.readbyqxmd.com/read/29416747/cd34-human-placenta-derived-mesenchymal-stem-cells-protect-against-heat-stroke-mortality-in-rats
#19
Willie Lin, Yogi Chang-Yo Hsuan, Yu-Chin Su, Cheng-Hsien Lin, Mao-Tsun Lin, Zi-Hao Chen, Ching-Ping Chang, Kao-Chang Lin
CD34 is a transmembrane phosphoglycoprotein used to selectively enrich bone marrow in hematopoietic stem cells for transplantation. Treating rats with CD34+ cells derived from human umbilical cord blood before or after heat stroke has been shown to promote survival. We investigated whether CD34- human placenta-derived stem cells (PDMSCs) could improve survival following heat stroke in rats. Rats were subjected to heat stress (42°C for 98 min) to induce heat stroke. Intravenous administration of PDMSCs 1 day before or immediately after the onset of heat stroke improved survival by 60% and 20%, respectively...
January 5, 2018: Oncotarget
https://www.readbyqxmd.com/read/29416638/nicotine-enhanced-stemness-and-epithelial-mesenchymal-transition-of-human-umbilical-cord-mesenchymal-stem-cells-promote-tumor-formation-and-growth-in-nude-mice
#20
Tao Li, Jun Zhang, Jiahui Zhang, Nannan Zhang, Yang Zeng, Shengnan Tang, Zehua Tao, Xiying Qu, Jue Jia, Wei Zhu, Xiaochun Sun, Huabiao Chen
Cigarette smoking is a well-known risk factor in the development and progression of malignant diseases. Nicotine, the major constituent in cigarette smoke, has also shown negative effects on stem cells. Mesenchymal stem cells (MSCs) have been widely demonstrated to migrate into tumors and play key roles in cancer progression. However, the mechanisms by which nicotine impacts MSCs and tumorigenesis of lung cancer are still undetermined. In this study we investigated the effects of nicotine on human umbilical cord mesenchymal stem cells (hUC-MSCs) and the impacts of nicotine-treated hUC-MSCs on tumor formation and progression...
January 2, 2018: Oncotarget
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