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umbilical cord stem cells

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https://www.readbyqxmd.com/read/29053065/investigation-of-the-role-of-alginate-containing-high-guluronic-acid-on-osteogenic-differentiation-capacity-of-human-umbilical-cord-wharton-s-jelly-mesenchymal-stem-cells
#1
Darioush Bijan Nejad, Saeed Azandeh, Rezvan Habibi, Vahid Bayati, Kambiz Ahmadi Angali
BACKGROUND AND AIM: Cell encapsulation using biodegradable material has promising results for tissue engineering. Since pressure is an effective factor on stem cell behavior and various concentrations of alginate create different pressures on the cells, therefore our goal was to evaluate the mechanical effect of 1/2% (w/v) and 1/8%(w/v) alginate containing high guluronic acid on viability and osteogenic capacity of HUCWJ cells. METHODS: Cell viability was evaluated by MTT assay after 1, 7 and 14 days...
October 20, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/29050787/transplantation-routes-affect-the-efficacy-of-human-umbilical-cord-mesenchymal-stem-cells-in-a-rat-gdm-model
#2
Dan Wu, Shan Zou, Haibin Chen, Xiaoyan Li, Yetao Xu, Qing Zuo, Yi Pan, Shi-Wen Jiang, Huan Huang, Lizhou Sun
Gestational diabetes mellitus (GDM) is harmful to both the mother and fetus. Although transplantation of human umbilical cord mesenchymal stem cells (HUMSCs) could be a useful therapy for GDM, the influences of different transplantation routes on the therapeutic effects remain unclear. In this study, we isolated and cultured the HUMSCs for transplantation, and the biological activity of HUMSCs was verified by flow cytometric analysis (the positive markers, CD44, CD73, CD105 and CD90, the negative markers, CD45, CD34, CD19, HLA-DR, and CD11b) and potency of osteogenic, adipogenic and chondrogenic differentiation...
October 16, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29050516/human-bone-marrow-mesenchymal-stromal-cell-derived-osteoblasts-promote-the-expansion-of-hematopoietic-progenitors-through-beta-catenin-and-notch-signaling-pathways
#3
Matthew Michalicka, Gavin Boisjoli, Suria Jahan, Owen Hovey, Emily Doxtator, Ahmad Abu-Khader, Roya Pasha, Nicolas Pineault
Coculture of hematopoietic stem cells (HSC) with primary stromal cells from HSC niches supports the maintenance and expansion of HSC and progenitors ex vivo. However, a major drawback is the availability of primary human samples for research and clinical applications. We investigated the use of in vitro derived osteoblasts as a new source of feeder cells and characterized the molecular pathways that mediate their growth promoting activities. First, we compared the growth and differentiation modulating activities of mesenchymal stromal cells (MSC)-derived osteoblasts (M-OST) to that of their undifferentiated precursor on umbilical cord blood (UCB) progenitors...
October 19, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29048109/evaluation-of-a-disease-risk-index-for-adult-patients-undergoing-umbilical-cord-blood-transplantation-for-haematological-malignancies
#4
Annalisa Paviglianiti, Annalisa Ruggeri, Fernanda Volt, Guillermo Sanz, Noel Milpied, Sabine Furst, Albert Esquirol, William Arcese, Alessandra Picardi, Christelle Ferra, Norbert Ifrah, Jean H Bourhis, Kavita Raj, Peter A von dem Borne, Simona Sica, Anne-Lise Menard, Adrian Bloor, Chantal Kenzey, Eliane Gluckman, Vanderson Rocha
A disease risk index (DRI) has been defined for stratifying heterogeneous cohorts of patients undergoing allogeneic haematopoietic stem cell transplantation (HSCT). This index defines 4 distinct groups with different outcomes, dividing patients by disease type and status and considering cytogenetics for acute myeloid leukaemia and myelodysplastic syndromes (MDS). Recently, the DRI has been refined to include rare diseases and improve MDS stratification by blast percentage and response to prior therapy. Previous reports on DRI include only a small number of UCBT recipients...
October 19, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/29046200/-readmission-of-children-with-bronchopulmonary-dysplasia-in-the-first-2-years-of-life-a-clinical-analysis-of-121-cases
#5
Jing-Yi You, Chang Shu, Cai-Hui Gong, Sha Liu, Zhou Fu
OBJECTIVE: To investigate the clinical features of readmitted children with bronchopulmonary dysplasia (BPD) in the first 2 years of life. METHODS: A retrospective analysis was performed for the clinical data of 242 children with BPD who were readmitted due to recurrent lower respiratory tract infection (LRTI) in the first 2 years of life. RESULTS: Among all the 242 children with BPD, 115(47.5%) had wheezing, and the children aged 1-2 years had a significantly higher incidence rate of wheezing than those aged less than 1 year (P<0...
October 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/29042945/human-umbilical-cord-mesenchymal-stem-cells-increase-interleukin-9-production-of-cd4-t-cells
#6
Zhou Xin Yang, Ying Chi, Yue Ru Ji, You Wei Wang, Jing Zhang, Wei Feng Luo, Li Na Li, Cai Dong Hu, Guang Sheng Zhuo, Li Fang Wang, Zhi-Bo Han, Zhong Chao Han
Mesenchymal stem cells (MSC) are able to differentiate into cells of multiple lineage, and additionally act to modulate the immune response. Interleukin (IL)-9 is primarily produced by cluster of differentiation (CD)4(+) T cells to regulate the immune response. The present study aimed to investigate the effect of human umbilical cord derived-MSC (UC-MSC) on IL-9 production of human CD4(+) T cells. It was demonstrated that the addition of UC-MSC to the culture of CD4(+) T cells significantly enhanced IL-9 production by CD4(+) T cells...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29037940/hyperbaric-oxygen-treatment-effects-on-in-vitro-cultured-umbilical-cord-blood-cd34-cells
#7
Kiang Yien Cheung, Abigale Berry, Dandan Li, Omar S Aljitawi
BACKGROUND AIMS: Umbilical cord blood (UCB) provides an alternative source for hematopoietic stem/progenitor cells (HSPCs) in the treatment of hematological malignancies. However, clinical usage is limited due to the low quantity of HSPCs in each unit of cord blood and defects in bone marrow homing. Hyperbaric oxygen (HBO) is among the more recently explored methods used to improve UCB homing and engraftment. HBO works by lowering the host erythropoietin before UCB infusion to facilitate UCB HSPC homing, because such UCB cells are not directly exposed to HBO...
October 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29037555/a-genome-wide-comparison-of-mesenchymal-stem-cells-derived-from-human-placenta-and-umbilical-cord
#8
Sen-Wen Teng, Yu-Shu Lo, Wei-Ting Liu, Yogi Hsuan, Willie Lin
OBJECTIVE: The human umbilical cord and placenta have been considered as attractive alternative sources for noninvasive isolation of human mesenchymal stem cells (hMSCs). Different sources of MSC may have individual differentiation potential and phenotype. In this study, we compared the genome-wide expression data of umbilical cord and placenta derived hMSCs to identify specific differential expression genes (DEGs) and corresponding functions. MATERIALS AND METHODS: We collected human placental tissues and umbilical cord from healthy full-term placenta (n = 17)...
October 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29035685/mechanistic-effect-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-on-the-submandibular-salivary-gland-in-ovariectomized-rats
#9
Zienab Gouda, Mohamed Khalifa, Sally Shalaby, Samia Hussein
To reveal the effect of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on the submandibular gland after bilateral ovariectomy, 21 adult Sprague-Dawley female rats were divided equally into three groups: sham-operated group (SHAM), ovariectomized group (OVX) and OVX received repeated intravenous injection of hUCB-MSCs group (OVX + hUCB-MSCs). RT-PCR for AQP3, AQP4, AQP5 and BMP6 gene expression was determined. The cellular localization and expression of anti-human CD105 anti-human CD34, anti-proliferating nuclear antigen (PCNA), anti-single stranded DNA(ssDNA), anti-Caspase 3, anti-aquaporin 1(AQP1) and anti-α smooth muscle actin(α-SMA) were determined immunohistochemically...
October 16, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29035086/notch-signaling-is-essential-for-maturation-self-renewal-and-tri-differentiation-of-in-vitro-derived-human-neural-stem-cells
#10
Katari Venkatesh, L Vinod Kumar Reddy, Salar Abbas, Madhubanti Mullick, Erfath Thanjeem Begum Moghal, Janardhana Papayya Balakrishna, Dwaipayan Sen
Although neural stem cells (NSCs) have potential applications in treating neurological disorders, much still needs to be understood about the differentiation biology for their successful clinical translation. In this study, we aimed at deriving NSCs from human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) and explored the role of Notch signaling in the differentiation process. The hUCB-MSCs were characterized as per guidelines of the International Society of Cellular Therapy. NSCs were successfully generated from hUCB-MSCs by using epidermal and fibroblast growth factors under serum-free conditions...
October 16, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/29023156/stem-cell-therapy-for-articular-cartilage-repair-review-of-the-entity-of-cell-populations-used-and-the-result-of-the-clinical-application-of-each-entity
#11
Yong-Beom Park, Chul-Won Ha, Ji Heon Rhim, Han-Jun Lee
BACKGROUND: Following successful preclinical studies, stem cell therapy is emerging as a candidate for the treatment of articular cartilage lesions. Because stem cell therapy for cartilage repair in humans is at an early phase, confusion and errors are found in the literature regarding use of the term stem cell therapy in this field. PURPOSE: To provide an overview of the outcomes of cartilage repair, elucidating the various cell populations used, and thus reduce confusion with regard to using the term stem cell therapy...
October 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29021466/successful-treatment-of-a-case-of-late-onset-colitis-after-umbilical-cord-transplantation-with-metronidazole-a-case-report-and-literature-review
#12
Reona Sakemura, Hiroshi Hayakawa, Hiroatsu Iida, Masafumi Ito, Tomohiro Kajiguchi
Diarrhea after hematopoietic stem cell transplantation (HSCT) can be life-threatening, and its etiology includes conditioning regimens, graft-versus-host disease (GVHD), infections, and transplantation-associated microangiopathy (iTAM). Cord colitis syndrome (CCS) has been described as a syndrome of culture-negative and antibiotic-responsive persistent watery and non-bloody diarrhea of uncertain pathogenesis and occurs in umbilical cord blood transplantation (UCBT) recipients. We encountered a case similar to CCS that developed severe watery diarrhea after UCBT without any signs of GVHD or infection and responded well to metronidazole (MNZ) treatment...
October 11, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29019171/optimization-of-culture-conditions-for-rapid-clinical-scale-expansion-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells
#13
Wankyu Choi, Soon-Jae Kwon, Hye Jin Jin, Sang Young Jeong, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Hong Bae Jeon, Eun Su Jeon
BACKGROUND: Mesenchymal stem cells (MSCs) have broad-spectrum therapeutic effects in various diseases, and thus have many clinical applications. However, it is difficult to produce sufficient numbers of MSCs for clinical use, and improved culture systems are required. Here, we report the effects of calcium (Ca(2+)) and hypoxia on the proliferation of human umbilical cord blood-derived MSCs (hUCB-MSCs). In addition, we determined the optimal conditions of these two factors for the large-scale culture of hUCB-MSCs...
October 10, 2017: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/29017529/short-review-on-human-umbilical-cord-lining-epithelial-cells-and-their-potential-clinical-applications
#14
REVIEW
Razwa Saleh, Hasan Mahmud Reza
BACKGROUND: The human umbilical cord has been studied extensively in the past two decades. It is free of ethical dilemmas, non-tumorigenic, and less immunogenic and thus provides a significant advantage over other stem cell sources. The cord lining yields both mesenchymal and epithelial stem cells. The mesenchymal cells have been appraised at length by many researchers, which led to the current review focusing on the cord lining epithelial cells (CLECs). These cells have high proliferative capacity and their superior harvest and multiplication, using the revolutionary CellOptima(TM) technology, makes them better candidates in comparison to contemporary adult stem cells...
October 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29016557/future-perspectives-of-cell-therapy-for-neonatal-hypoxic-ischemic-encephalopathy
#15
REVIEW
Makoto Nabetani, Haruo Shintaku, Takashi Hamazaki
Neonatal ischemic brain injury causes permanent motor-deficit cerebral palsy. Hypoxic-ischemic encephalopathy (HIE) is a very serious condition that can result in death and disability. In 1997, we reported that irreversible neuronal cell damage is induced by the elevation of intracellular Ca ion concentration that has occurred in sequence after excess accumulation of the excitatory neurotransmitter glutamate during ischemia. We also reported that hypothermia was effective in treating ischemic brain damage in rats by suppressing energy loss and raising intracellular Ca ion concentration...
October 10, 2017: Pediatric Research
https://www.readbyqxmd.com/read/29016394/mir-20b-106a-modulate-ngn2-gene-expression-during-neural-differentiation-of-human-umbilical-cord-mesenchymal-stem-cells
#16
Haoyu Wang, Wenrui Ban, Tao Wang, Zhuo Li, Xiaoqian Dang
The aim of this study was to perform neural differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord (HUMSCs) and explore the role of miR-20b and miR-106a, which may regulate Neurogenin-2 (Ngn2) expression during the neural differentiation. HUMSCs were cultured and induced in vitro. The cells were stained for nestin and microtubule-associated protein 2 (MAP2) by immunofluorescence. The interactional binding sites between the 3'-untranslated region (3'-UTR) of Ngn2 mRNA and miR-20b or miR-106a were predicted by bioinformatics and identified by a dual-luciferase assay...
October 9, 2017: Neuroreport
https://www.readbyqxmd.com/read/28994199/inhibition-of-mek-erk-signalling-pathway-promotes-erythroid-differentiation-and-reduces-hscs-engraftment-in-ex%C3%A2-vivo-expanded-haematopoietic-stem-cells
#17
Morteza Zarrabi, Elaheh Afzal, Mohammad Hossein Asghari, Monireh Mohammad, Hamidreza Aboulkheyr Es, Marzieh Ebrahimi
The MEK/ERK pathway is found to be important in regulating different biological processes such as proliferation, differentiation and survival in a wide variety of cells. However, its role in self-renewal of haematopoietic stem cells is controversial and remains to be clarified. The aim of this study was to understand the role of MEK/ERK pathway in ex vivo expansion of mononuclear cells (MNCs) and purified CD34(+) cells, both derived from human umbilical cord blood (hUCB). Based on our results, culturing the cells in the presence of an inhibitor of MEK/ERK pathway-PD0325901 (PD)-significantly reduces the expansion of CD34(+) and CD34(+)  CD38(-) cells, while there is no change in the expression of stemness-related genes (HOXB4, BMI1)...
October 10, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28992805/the-effects-of-liquid-crystal-based-composite-substrates-on-cell-functional-responses-of-human-umbilical-cord-derived-mesenchymal-stem-cells-by-mechano-regulatory-process
#18
Hao Wu, Yupan Shang, Jiaqing Zhang, Lek Hang Cheang, Xiaoli Zeng, Mei Tu
Physical properties of extracellular matrix, including elasticity and microstructure, have been considered as important factors inducing stem cell differentiation. This study developed a novel type of liquid crystal-based matrix by combining the elastic property of polyurethane with viscoelastic liquid crystal to generate a soft elastic response resembling physical microenvironment of stem cell niche, and explored the mechano-driving cell behaviors. Addition of varying liquid crystal concentration (10 wt%, 30 wt% and 50 wt%) had great effects on surface morphology and elastic modulus of liquid crystal/ polyurethane composite substrates...
October 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28988272/pre-culture-in-endothelial-growth-medium-enhances-the-angiogenic-properties-of-adipose-derived-stem-stromal-cells
#19
Lucas E B Souza, Liziane R Beckenkamp, Lays M Sobral, Daianne M C Fantacini, Fernanda U F Melo, Josiane S Borges, Andréia M Leopoldino, Simone Kashima, Dimas Tadeu Covas
Considerable progress has been made on the development of adipose-derived stem/stromal cells (ASCs) as pro-angiogenic therapeutic tools. However, variable clinical results highlight the need for devising strategies to enhance their therapeutic efficacy. Since ASCs proliferate and stabilize newly formed vessels during the angiogenic phase of adipose tissue formation, we hypothesized that mimicking an angiogenic milieu during culture of ASCs would enhance their capacity to support endothelial cell survival and angiogenesis...
October 7, 2017: Angiogenesis
https://www.readbyqxmd.com/read/28985529/multiplex-crispr-cas9-based-genome-editing-in-human-hematopoietic-stem-cells-models-clonal-hematopoiesis-and-myeloid-neoplasia
#20
Zuzana Tothova, John M Krill-Burger, Katerina D Popova, Catherine C Landers, Quinlan L Sievers, David Yudovich, Roger Belizaire, Jon C Aster, Elizabeth A Morgan, Aviad Tsherniak, Benjamin L Ebert
Hematologic malignancies are driven by combinations of genetic lesions that have been difficult to model in human cells. We used CRISPR/Cas9 genome engineering of primary adult and umbilical cord blood CD34(+) human hematopoietic stem and progenitor cells (HSPCs), the cells of origin for myeloid pre-malignant and malignant diseases, followed by transplantation into immunodeficient mice to generate genetic models of clonal hematopoiesis and neoplasia. Human hematopoietic cells bearing mutations in combinations of genes, including cohesin complex genes, observed in myeloid malignancies generated immunophenotypically defined neoplastic clones capable of long-term, multi-lineage reconstitution and serial transplantation...
October 5, 2017: Cell Stem Cell
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