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https://www.readbyqxmd.com/read/28717315/seroprevalence-of-antibodies-to-astrovirus-in-chickens-in-grenada-west-indies
#1
Ravindra Nath Sharma, Romane Dufayet, Thomas Maufras, Kathryn O' Connell, Keshaw Tiwari
AIM: Chicken astroviruses (CAstV) are known to cause mild gastroenteritis, growth depression, and even mortality in poultry, especially in chickens, turkeys, and ducks. To the best our knowledge, there is no published information on CAstV in Grenada. This study was conducted to determine the prevalence of astrovirus in chickens in Grenada. MATERIALS AND METHODS: Blood samples from 366 indigenous chickens and 92 commercial chicken layers were collected from all parishes of the island and tested for antibodies against CAstV using commercial enzyme-linked immunosorbent assay...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28715672/genomic-and-pathogenic-analysis-of-a-muscovy-duck-parvovirus-strain-causing-short-beak-and-dwarfism-syndrome-without-tongue-protrusion
#2
Qiuling Fu, Yu Huang, Chunhe Wan, Guanghua Fu, Baomin Qi, Longfei Cheng, Shaohua Shi, Hongmei Chen, Rongchang Liu, Zhenhai Chen
In 2008, clinical cases of short beak and dwarfism syndrome (SBDS) caused by Muscovy duck parvovirus (MDPV) infection were found in mule duck and Taiwan white duck farms in Fujian, China. A MDPV LH strain causing duck SBDS without tongue protrusion was isolated in this study. Phylogenetic analysis show that the MDPV LH strain was clustered together with other MDPV strains, but divergent from GPV isolates. Two major fragment deletions were found in the inverted terminal repeats (ITR) of MDPV LH similar to the ones in the ITR of MDPV GX5, YY and SAAS-SHNH strains...
July 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28708585/the-neglected-avian-hepatotropic-virus-induces-acute-and-chronic-hepatitis-in-ducks-an-alternative-model-for-hepatology
#3
Xumin Ou, Sai Mao, Jingyu Cao, Yunchao Ma, Guangpeng Ma, Anchun Cheng, Mingshu Wang, Dekang Zhu, Shun Chen, Renyong Jia, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xiaoyue Chen
Duck Hepatitis A Virus (DHAV) belongs to the Avihepatovirus, which is also classified into Picornaviridae with Hepatovirus, Hepatitis A Virus (HAV). In humans, the pathogenesis of HAV is not well understood because of limited work with animal models. Here, we investigated the progress of duck viral hepatitis caused by DHAV and their potential for dissecting the pathogenesis of HAV. During the course of infection, the duck model had undergone hepatocellular lesions (vacuolation, acidophilic degeneration and steatosis), lymphocytes recruitment (neutrophil granulocytes, heterophilic granulocytes and T cells or plasm cells) and repair (activation of hepatic stellate cells, fibrosis and regeneration)...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28688454/reassortment-process-after-co-infection-of-pigs-with-avian-h1n1-and-swine-h3n2-influenza-viruses
#4
Kinga Urbaniak, Iwona Markowska-Daniel, Andrzej Kowalczyk, Krzysztof Kwit, Małgorzata Pomorska-Mól, Barbara Frącek, Zygmunt Pejsak
BACKGROUND: The influenza A virus is highly variable, which, to some degree, is caused by the reassortment of viral genetic material. This process plays a major role in the generation of novel influenza virus strains that can emerge in a new host population. Due to the susceptibility of pigs to infections with avian, swine and human influenza viruses, they are considered intermediate hosts for the adaptation of the avian influenza virus to humans. In order to test the reassortment process in pigs, they were co-infected with H3N2 A/swine/Gent/172/2008 (Gent/08) and H1N1 A/duck/Italy/1447/2005 (Italy/05) and co-housed with a group of naïve piglets...
July 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28685285/evaluation-of-the-replication-and-pathogenicity-of-a-variant-avian-paramyxovirus-serotype-6-in-mice
#5
Vuong Nghia Bui, Dai Quang Trinh, Lary N B Abao, Yuki Ozeki, Jonathan Runstadler, Kikuyasu Nakamura, Haruko Ogawa, Kunitoshi Imai
Avian paramyxoviruses (APMVs) have been evaluated for their potential use as vaccine vectors, sparking research efforts leading to a better understanding of APMVs' replication and pathogenicity. However, within APMV serotypes, significant genetic diversity exists, and the infectivity of variant strains in mammals has not been studied. We utilized a mouse model to evaluate the pathogenicity of a variant strain of APMV-6 (APMV-6/red-necked stint/Japan/8KS0813/2008) in comparison with the prototype APMV-6 strain (APMV-6/duck/Hong Kong/18/199/1977)...
July 6, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28674528/host-differences-affecting-resistance-and-susceptibility-of-the-second-generation-of-a-pekin-duck-flock-to-duck-hepatitis-a-virus-genotype-3
#6
Xiaoyan Wang, Jiaojiao Zhang, Runze Meng, Yong Jiang, Suyun Liang, Yunsheng Zhang, Ming Xie, Zhengkui Zhou, Shuisheng Hou
Earlier work suggested the possibility to anti duck hepatitis A virus genotype 3 (DHAV-3) using the resistance breeding strategy. Here, we report the creation of the second generations of a resistant Pekin duck flock (designated Z8R2) and a highly susceptible Pekin duck flock (designated Z8S2) and the investigation of their responses to DHAV-3. Experimental infection with DHAV-3 at 7 days of age resulted in a high mortality (66.3%) in 11 susceptible Z8S2 families and an extremely low mortality rate (2.67%) in 32 Z8R2 families, indicating that Z8R2 exhibits strong resistance to DHAV-3, while Z8S2 is highly susceptible to the virus...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28674238/characterization-of-duck-anas-platyrhynchos-mhc-class-i-gene-in-two-duck-lines
#7
Lin Zhang, Wei-Jie Liu, Jia-Qiang Wu, Min-Li Xu, Zheng-Jie Kong, Yan-Yan Huang, Shao-Hua Yang
To enrich gene polymorphism ofDuMHCI and provide data for further studies on disease resistance, 14DuMHCI genes from Weishan Ma duck and Cherry Valley duck were cloned, and their characterization were investigated. The overall conservation of the 14 alleles could be observed within the sequences, and relative conservation were also displayed in the peptide-binding domain and CD8 interaction sites. Based on full-length amino acid homology, MHC class I from different duck lines could be divided into 13 gene groups and three novel gene groups existed...
June 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28666650/functional-characterization-of-duck-lsm14a-in-ifn-%C3%AE-induction
#8
Kexin Hua, Huilin Li, Huanchun Chen, Mohamed Frahat Foda, Rui Luo, Hui Jin
Human LSm14A is a key component of processing body (P-body) assembly that mediates interferon-β (IFN-β) production by sensing viral RNA or DNA. To the best of our knowledge, we are the first to report duck LSm14A (duLSm14A) cloning from duck embryo fibroblasts (DEFs). Full-length duLSm14A encoded 461 amino acids and was highly homologous with chicken and swan goose sequences. More interestingly, the duLSm14A mRNA was extensively expressed in all the studied tissues. In DEFs, duLSm14A was localized in the cytoplasm as P-body-like dots...
June 27, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28640898/competition-between-influenza-a-virus-subtypes-through-heterosubtypic-immunity-modulates-re-infection-and-antibody-dynamics-in-the-mallard-duck
#9
Neus Latorre-Margalef, Justin D Brown, Alinde Fojtik, Rebecca L Poulson, Deborah Carter, Monique Franca, David E Stallknecht
Our overall hypothesis is that host population immunity directed at multiple antigens will influence the prevalence, diversity and evolution of influenza A virus (IAV) in avian populations where the vast subtype diversity is maintained. To investigate how initial infection influences the outcome of later infections with homologous or heterologous IAV subtypes and how viruses interact through host immune responses, we carried out experimental infections in mallard ducks (Anas platyrhynchos). Mallards were pre-challenged with an H3N8 low-pathogenic IAV and were divided into six groups...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28636622/inhibition-of-hepatitis-b-viral-entry-by-nucleic-acid-polymers-in-heparg-cells-and-primary-human-hepatocytes
#10
Clément Guillot, Nora Martel, Françoise Berby, Isabelle Bordes, Olivier Hantz, Matthieu Blanchet, Camille Sureau, Andrew Vaillant, Isabelle Chemin
Hepatitis B virus (HBV) infection remains a major public health concern worldwide with 240 million individuals chronically infected and at risk of developing cirrhosis and hepatocellular carcinoma. Current treatments rarely cure chronic hepatitis B infection, highlighting the need for new anti-HBV drugs. Nucleic acid polymers (NAPs) are phosphorothioated oligonucleotides that have demonstrated a great potential to inhibit infection with several viruses. In chronically infected human patients, NAPs administration lead to a decline of blood HBsAg and HBV DNA and to HBsAg seroconversion, the expected signs of functional cure...
2017: PloS One
https://www.readbyqxmd.com/read/28634474/different-dynamic-distribution-in-chickens-and-ducks-of-the-hypervirulent-novel-genotype-fowl-adenovirus-serotype-4-recently-emerged-in-china
#11
Qing Pan, Yanchao Yang, Zhibin Shi, Linlin Liu, Yulong Gao, Xiaole Qi, Changjun Liu, Yanping Zhang, Hongyu Cui, Xiaomei Wang
A hypervirulent fowl adenovirus serotype 4 (FAdV-4) has caused hepatitis-hydropericardium syndrome (HHS) with mortalities that range from 30 to 80% in outbreaks across China since 2015. The FAdV-4 strain was characterized as a novel genotype based on the specific genome characteristics. However, our understanding of the dynamic distribution, tissue tropism, and pathogenesis of the novel FAdV-4 is incomplete. In this study, a new, sensitive and FAdV-4-specific real-time PCR was developed and applied to detect the dynamic distribution of the duck origin, novel FAdV-4 strain HLJDAd15 in experimentally infected special-pathogen free (SPF) chickens and ducks...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28627206/investigation-on-the-endemic-characteristics-of-metorchis-orientalisin-huainan-area-china
#12
Xiaodong Zhan, Chaopin Li, Hua Wu, Entao Sun, Yuxia Zhu
OBJECTIVE: To investigate the endemic characteristics of Metorchis orientalis (M. orientalis)in the Huainan area, Anhui province, China. METHODS: The first-intermediate host, second-intermediate host and reservoir hosts were collected, and the endemic characteristics of M. orientalis were examined through field investigation and artificial infection. RESULTS: Investigation was completed in 89 domestic ducks, 156 domestic chicken, 41 domestic geese, 20 domestic cats and 19 dogs...
June 5, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28626158/ducks-as-a-potential-reservoir-for-pasteurella-multocida-infection-detected-using-a-new-romph-based-elisa
#13
Rongchang Liu, Cuiteng Chen, Longfei Cheng, Ronghui Lu, Guanghua Fu, Shaohua Shi, Hongmei Chen, Chunhe Wan, Jiansheng Lin, Qiuling Fu, Yu Huang
Pasteurella multocida is an important pathogen of numerous domestic poultry and wild animals and is associated with a variety of diseases including fowl cholera. The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) based on recombinant outer-membrane protein H (rOmpH) for detection of anti-P. multocida antibodies in serum to determine their prevalence in Chinese ducks. The P. multocida ompH gene was cloned into pET32a, and rOmpH was expressed in Escherichia coli BL21 (DE3)...
June 15, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28622015/the-first-whole-genome-sequence-and-pathogenicity-characterization-of-a-fowl-adenovirus-4-isolated-from-ducks-associated-with-inclusion-body-hepatitis-and-hydropericardium-syndrome
#14
Qing Pan, Linlin Liu, Yongqiang Wang, Yanping Zhang, Xiaole Qi, Changjun Liu, Yulong Gao, Xiaomei Wang, Hongyu Cui
In June 2015, an infectious disease with high prevalence causing severe hydropericardium syndrome (HPS) first appeared in duck farms of northeast China. The disease showed high morbidity of 35% and mortality of 15% in a commercial duck farm with 200,000 45-day-old ducks. One strain of hypervirulent fowl adenovirus serotype 4 was identified and designated as HLJDAd15. The whole genome of the duck isolate was sequenced and found to contain the same large deletions as a genotype that has become prevalent in chickens in China recently, indicating that this disease might be transmitted from chickens to ducks...
June 16, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28620809/single-gene-reassortment-of-highly-pathogenic-avian-influenza-a-h5n1-in-the-low-pathogenic-h9n2-backbone-and-its-impact-on-pathogenicity-and-infectivity-of-novel-reassortant-viruses
#15
Yassmin Moatasim, Ahmed Kandeil, Ahmed Mostafa, Sary Khaleel Abd Elghaffar, Rabeh El Shesheny, Ahmed Helmy M Elwahy, Mohamed Ahmed Ali
Avian influenza A H5N1 and H9N2 viruses have been extensively circulating in various avian species and frequently infect mammals, including humans. The synchronous circulation of both viruses in Egypt provides an opportunity for possible genetic assortment, posing a probable threat to global public health. To assess the potential risk of the IAV reassortants derived from co-circulation of these two AI subtypes, reverse genetics technology was used to generate a set of IAV reassortants carrying single genetic segments of clade 2...
June 15, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28620271/glutamine-ameliorates-mucosal-damage-caused-by-immune-responses-to-duck-plague-virus
#16
Yuanyuan Zhang, Lili Zhao, Yan Zhou, Chenxi Diao, Lingxia Han, Niu Yinjie, Shengwang Liu, Hongyan Chen
The immune-releasing effects of L-glutamine (Gln) supplementation in duck plague virus (DPV)-infected ducklings were evaluated in 120 seven-day-old ducklings that were divided into 8 groups. The ducklings in control and DPV, 0.5Gln and DPV + 0.5Gln, 1.0Gln and DPV + 1.0Gln, and 2.0Gln and DPV + 2.0Gln received 0, 0.5, 1.0, and 2.0 g of Gln/kg feed/d by gastric perfusion, respectively. Then, the ducklings in control to 2.0Gln were injected with 0.2 mL of phosphate-buffered saline, while those in DPV to DPV + 2...
April 2017: Dose-response: a Publication of International Hormesis Society
https://www.readbyqxmd.com/read/28619174/experimental-infection-with-highly-pathogenic-h5n8-avian-influenza-viruses-in-the-mandarin-duck-aix-galericulata-and-domestic-pigeon-columba-livia-domestica
#17
Jung-Hoon Kwon, Yun Kyung Noh, Dong-Hun Lee, Seong-Su Yuk, Tseren-Ochir Erdene-Ochir, Jin-Yong Noh, Woo-Tack Hong, Jei-Hyun Jeong, Sol Jeong, Gyeong-Bin Gwon, Chang-Seon Song, Sang-Soep Nahm
Wild birds play a major role in the evolution, maintenance, and dissemination of highly pathogenic avian influenza viruses (HPAIV). Sub-clinical infection with HPAI in resident wild birds could be a source of dissemination of HPAIV and continuous outbreaks. In this study, the pathogenicity and infectivity of two strains of H5N8 clade 2.3.4.4 virus were evaluated in the Mandarin duck (Aix galericulata) and domestic pigeon (Columba livia domestica). None of the birds experimentally infected with H5N8 viruses showed clinical signs or mortality...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28619152/the-newly-emerging-duck-origin-goose-parvovirus-in-china-exhibits-a-wide-range-of-pathogenicity-to-main-domesticated-waterfowl
#18
Shifeng Xiao, Shilong Chen, Xiaoxia Cheng, Fengqiang Lin, Shao Wang, Xiaoli Zhu, Bo Yu, Meiqing Huang, Jinxiang Wang, Nanyang Wu, Min Zheng, Shaoying Chen, Fusong Yu
Short beak and dwarfism syndrome virus (SBDSV) is a newly emerging distinct duck-origin goose parvovirus that belongs to the genus Dependovirus. Our previous studies have found that SBDSV was highly pathogenic to Cherry Valley ducklings and mule ducklings. However, little is known about its pathogenicity to other waterfowls. In the present study, the pathogenicity of SBDSV was evaluated in domesticated waterfowl including Muscovy ducklings, Sheldrake ducklings and domestic goslings. All experimentally infected birds exhibited remarkable growth retardation, anorexia and diarrhea similar to naturally infected birds...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28592320/infectivity-transmission-and-pathogenicity-of-h5-highly-pathogenic-avian-influenza-clade-2-3-4-4-h5n8-and-h5n2-united-states-index-viruses-in-pekin-ducks-and-chinese-geese
#19
Mary J Pantin-Jackwood, Mar Costa-Hurtado, Kateri Bertran, Eric DeJesus, Diane Smith, David E Swayne
In late 2014, a H5N8 highly pathogenic avian influenza (HPAI) virus, clade 2.3.4.4, spread by migratory waterfowl into North America reassorting with low pathogenicity AI viruses to produce a H5N2 HPAI virus. Since domestic waterfowl are common backyard poultry frequently in contact with wild waterfowl, the infectivity, transmissibility, and pathogenicity of the United States H5 HPAI index viruses (H5N8 and H5N2) was investigated in domestic ducks and geese. Ducks infected with the viruses had an increase in body temperature but no or mild clinical signs...
June 7, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28571695/influenza-viruses-production-evaluation-of-a-novel-avian-cell-line-duckcelt%C3%A2-t17
#20
Emma Petiot, Anaïs Proust, Aurélien Traversier, Laurent Durous, Frédéric Dappozze, Marianne Gras, Chantal Guillard, Jean-Marc Balloul, Manuel Rosa-Calatrava
The influenza vaccine manufacturing industry is looking for production cell lines that are easily scalable, highly permissive to multiple viruses, and more effective in term of viral productivity. One critical characteristic of such cell lines is their ability to grow in suspension, in serum free conditions and at high cell densities. Influenza virus causing severe epidemics both in human and animals is an important threat to world healthcare. The repetitive apparition of influenza pandemic outbreaks in the last 20years explains that manufacturing sector is still looking for more effective production processes to replace/supplement embryonated egg-based process...
May 29, 2017: Vaccine
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