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https://www.readbyqxmd.com/read/28807852/extracts-of-equisetum-giganteum-l-and-copaifera-reticulate-ducke-show-strong-antiviral-activity-against-the-sexually-transmitted-pathogen-herpes-simplex-virus-type-2
#1
Marianela Patzi Churqui, Liza Lind, Karolina Thörn, Alexandra Svensson, Otto Savolainen, Katty Terrazas Aranda, Kristina Eriksson
ETHNOPHARMACOLOGICAL RELEVANCE: Equisetum giganteum L and Copaifera reticulate Ducke have been traditionally used by women of the Tacana tribe in the Bolivian Amazonas for genital hygiene and for treatment of genital infection/inflammation. AIM OF THE STUDY: To assess the ability of extracts from Equisetum giganteum L and Copaifera reticulate Ducke to block genital viral infection by herpes simplex virus type 2. MATERIALS AND METHODS: Equisetum giganteum L and Copaifera reticulate Ducke were collected from the Amazon region of La Paz, Bolivia...
August 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28805531/fabrication-and-characterization-of-the-porous-duck-s-feet-collagen-sponge-for-wound-healing-applications
#2
Md Tipu Sultan, Ju Yeon Jeong, Ye Bin Seo, Ok Joo Lee, Hyung Woo Ju, Hyun Jung Park, Young Jin Lee, Ji Seung Lee, Soon Hee Kim, Chan Hum Park
There are several artificial dermis commonly use to cover the wound and promote healing. The major goal of wound management is fast and scarless healing. However, there is no ideal skin substitute, that is effective to accelerate wound healing without scar formation. Artificial dermis substitute also has some drawbacks, such as high cost, insufficient available period and donor pathogen infection. To overcome these problems, we developed duck's feet collagen (DFC) sponge as artificial dermal substitutes for the treatment of full-thickness skin defects...
August 14, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28780326/downregulation-of-inflammatory-cytokines-by-berberine-attenuates-riemerella-anatipestifer-infection-in-ducks
#3
Cherry P Fernandez, Fahmida Afrin, Rochelle A Flores, Woo H Kim, Jipseol Jeong, Suk Kim, Hong H Chang, Hyun S Lillehoj, Wongi Min
Riemerella anatipestifer, an important infectious bacterium affecting the duck industry, has 5-75% mortality, depending on strain virulence. We previously demonstrated that proinflammatory cytokines are involved in inflammation during, and regulating susceptibility to, R. anatipestifer infection. We investigated the effects of the anti-inflammatory compound berberine in duck splenic lymphocytes stimulated with killed R. anatipestifer, and in R. anatipestifer-infected ducks. IL-17A, IL-17F, and IL-1β transcripts were downregulated, and IFN-γ and IL-10 transcripts enhanced, in berberine-treated stimulated splenic lymphocytes, compared to stimulated untreated splenic lymphocytes...
August 2, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28780191/significant-inhibition-of-tembusu-virus-envelope-and-ns5-gene-using-an-adenovirus-mediated-short-hairpin-rna-delivery-system
#4
Hongzhi Wang, Qiang Feng, Lei Wei, Liling Zhuo, Hao Chen, Youxiang Diao, Yi Tang
Tembusu virus (TMUV) is a mosquito-borne flavivirus, which was first isolated in the tropics during the 1970s. Recently, a disease characterized by ovarian haemorrhage and neurological symptoms was observed in ducks in China, which threatens poultry production. However, there is no suitable vaccination strategy or effective antiviral drugs to combat TMUV infections. Consequently, there is an urgent need to develop a new anti-TMUV therapy. In this study, we report an efficient short hairpin RNA (shRNA) delivery strategy for the inhibition of TMUV production using an adenovirus vector system...
August 3, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28771136/assessing-evidence-for-avian-to-human-transmission-of-influenza-a-h9n2-virus-in-rural-farming-communities-in-northern-vietnam
#5
Le Nguyen Minh Hoa, Nguyen Anh Tuan, Pham Ha My, Tran Thi Kieu Huong, Nguyen Thi Yen Chi, Trang Thi Hau Thu, Juan Carrique-Mas, Mai Thuy Duong, Nguyen Dang Tho, Nguyen Dang Hoang, To Long Thanh, Nguyen Thi Diep, Nguyen van Duong, Tran Khanh Toan, Trinh Son Tung, Le Quynh Mai, Munir Iqbal, Heiman Wertheim, H Rogier van Doorn, Juliet E Bryant, The Vizions Consortium
Rural farming communities in northern Vietnam do not routinely practice vaccination for influenza A viruses (IAV) for either humans or poultry, which enables us to study transmission intensity via seroepidemiology. Using samples from a longitudinal cohort of farming households, we determined the number of symptomatic and asymptomatic human infections for seasonal IAV and avian A/H9 over 2 years. As expected, we detected virologically confirmed acute cases of seasonal IAV in humans, as well as large numbers of subclinical seroconversions to A/H1pdm [55/265 (21 %)], A/H3 [95/265 (36 %)] and A/H9 [24/265 (9 %)]...
August 4, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28769080/few-basepairing-independent-motifs-in-the-apical-half-of-the-avian-hbv-%C3%AE%C2%B5-rna-stem-loop-determine-site-specific-initiation-of-protein-priming
#6
Markus Gajer, Katharina Dörnbrack, Christine Rösler, Bernadette Schmid, Jürgen Beck, Michael Nassal
Hepadnaviruses, including human hepatitis B virus (HBV), replicate their tiny DNA genomes by protein-primed reverse transcription of a pregenomic (pg) RNA. Replication initiation as well as pgRNA encapsidation depend on the interaction of the viral polymerase, P protein, with the ε RNA element, featuring a lower and an upper stem, a central bulge, and an apical loop. The bulge, somehow assisted by the loop, acts as template for a P protein-linked DNA oligo that primes full-length minus-strand DNA synthesis...
August 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747153/temporal-and-spatial-analysis-of-psittacosis-in-association-with-poultry-farming-in-the-netherlands-2000-2015
#7
Lenny Hogerwerf, Manon M C Holstege, Elisa Benincà, Frederika Dijkstra, Wim van der Hoek
BACKGROUND: Human psittacosis is a highly under diagnosed zoonotic disease, commonly linked to psittacine birds. Psittacosis in birds, also known as avian chlamydiosis, is endemic in poultry, but the risk for people living close to poultry farms is unknown. Therefore, our study aimed to explore the temporal and spatial patterns of human psittacosis infections and identify possible associations with poultry farming in the Netherlands. METHODS: We analysed data on 700 human cases of psittacosis notified between 01-01-2000 and 01-09-2015...
July 26, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28743080/the-role-of-hexon-in-egg-drop-syndrome-virus-edsv-inducing-apoptosis-in-duck-embryo-fibroblast-cells
#8
Xuefeng Qi, Jiamin Xu, Zugui Wang, Xueping Wang, Jingyu Wang
Although extensive efforts have been made to understand adenovirus infection in human cells, little is known for egg drop syndrome virus (EDSV) infection in the avian-derived cells. In this study, the effects of EDSV infection as well as the possible role hexon protein, the main building block of the EDSV capsid, on apoptosis induction in duck embryo fibroblast (DEF) cells was examined. Flow cytometry analysis and TUNEL assay revealed that EDSV infection induced significant apoptosis in DEF cells compared with mock infected cells...
July 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28741797/risk-factors-and-long-term-outcomes-of-parvovirus-b19-infection-in-kidney-transplant-patients
#9
Chung Hee Baek, Hyosang Kim, Won Soek Yang, Duck Jong Han, Su-Kil Park
BACKGROUND: Parvovirus B19 is a small, non-enveloped, single-stranded DNA virus with a special affinity for the erythroid progenitor cells of the bone marrow. The first case of parvovirus B19 infection in a kidney transplant recipient (KTR) was reported in 1986. Data on the risk factors and specific clinical characteristics of parvovirus B19 infection remain insufficient. METHODS: We screened 602 KTRs for parvovirus B19 infection using parvovirus B19 polymerase chain reaction (PCR) from January 1990 to April 2016, and the clinical characteristics of patients with positive results were compared to those of age- and gender-matched patients with negative PCR results...
July 25, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28728835/a-multiplex-pcr-for-detection-of-six-viruses-in-ducks
#10
Yongjuan Wang, Shanyuan Zhu, Weiming Hong, Anping Wang, Weiyong Zuo
In this study, six pairs of specific primers that can amplify DNA fragments of different sizes were designed and synthesized according to viral protein gene sequences published in GenBank. Then, a multiplex PCR method was established for rapid detection of duck hepatitis virus 1, duck plague virus, duck Tembusu virus, muscovy duck parvovirus, muscovy duck reovirus, and duck H9N2 avian influenza virus, and achieve simple and rapid detection of viral diseases in ducks. Single PCR was used to confirm primer specificity, and PCR conditions were optimized to construct a multiplex PCR system...
July 17, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28717315/seroprevalence-of-antibodies-to-astrovirus-in-chickens-in-grenada-west-indies
#11
Ravindra Nath Sharma, Romane Dufayet, Thomas Maufras, Kathryn O' Connell, Keshaw Tiwari
AIM: Chicken astroviruses (CAstV) are known to cause mild gastroenteritis, growth depression, and even mortality in poultry, especially in chickens, turkeys, and ducks. To the best our knowledge, there is no published information on CAstV in Grenada. This study was conducted to determine the prevalence of astrovirus in chickens in Grenada. MATERIALS AND METHODS: Blood samples from 366 indigenous chickens and 92 commercial chicken layers were collected from all parishes of the island and tested for antibodies against CAstV using commercial enzyme-linked immunosorbent assay...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28715672/genomic-and-pathogenic-analysis-of-a-muscovy-duck-parvovirus-strain-causing-short-beak-and-dwarfism-syndrome-without-tongue-protrusion
#12
Qiuling Fu, Yu Huang, Chunhe Wan, Guanghua Fu, Baomin Qi, Longfei Cheng, Shaohua Shi, Hongmei Chen, Rongchang Liu, Zhenhai Chen
In 2008, clinical cases of short beak and dwarfism syndrome (SBDS) caused by Muscovy duck parvovirus (MDPV) infection were found in mule duck and Taiwan white duck farms in Fujian, China. A MDPV LH strain causing duck SBDS without tongue protrusion was isolated in this study. Phylogenetic analysis show that the MDPV LH strain was clustered together with other MDPV strains, but divergent from GPV isolates. Two major fragment deletions were found in the inverted terminal repeats (ITR) of MDPV LH similar to the ones in the ITR of MDPV GX5, YY and SAAS-SHNH strains...
July 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28708585/the-neglected-avian-hepatotropic-virus-induces-acute-and-chronic-hepatitis-in-ducks-an-alternative-model-for-hepatology
#13
Xumin Ou, Sai Mao, Jingyu Cao, Yunchao Ma, Guangpeng Ma, Anchun Cheng, Mingshu Wang, Dekang Zhu, Shun Chen, Renyong Jia, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xiaoyue Chen
Duck Hepatitis A Virus (DHAV) belongs to the Avihepatovirus, which is also classified into Picornaviridae with Hepatovirus, Hepatitis A Virus (HAV). In humans, the pathogenesis of HAV is not well understood because of limited work with animal models. Here, we investigated the progress of duck viral hepatitis caused by DHAV and their potential for dissecting the pathogenesis of HAV. During the course of infection, the duck model had undergone hepatocellular lesions (vacuolation, acidophilic degeneration and steatosis), lymphocytes recruitment (neutrophil granulocytes, heterophilic granulocytes and T cells or plasm cells) and repair (activation of hepatic stellate cells, fibrosis and regeneration)...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28688454/reassortment-process-after-co-infection-of-pigs-with-avian-h1n1-and-swine-h3n2-influenza-viruses
#14
Kinga Urbaniak, Iwona Markowska-Daniel, Andrzej Kowalczyk, Krzysztof Kwit, Małgorzata Pomorska-Mól, Barbara Frącek, Zygmunt Pejsak
BACKGROUND: The influenza A virus is highly variable, which, to some degree, is caused by the reassortment of viral genetic material. This process plays a major role in the generation of novel influenza virus strains that can emerge in a new host population. Due to the susceptibility of pigs to infections with avian, swine and human influenza viruses, they are considered intermediate hosts for the adaptation of the avian influenza virus to humans. In order to test the reassortment process in pigs, they were co-infected with H3N2 A/swine/Gent/172/2008 (Gent/08) and H1N1 A/duck/Italy/1447/2005 (Italy/05) and co-housed with a group of naïve piglets...
July 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28685285/evaluation-of-the-replication-and-pathogenicity-of-a-variant-avian-paramyxovirus-serotype-6-in-mice
#15
Vuong Nghia Bui, Dai Quang Trinh, Lary N B Abao, Yuki Ozeki, Jonathan Runstadler, Kikuyasu Nakamura, Haruko Ogawa, Kunitoshi Imai
Avian paramyxoviruses (APMVs) have been evaluated for their potential use as vaccine vectors, sparking research efforts leading to a better understanding of APMVs' replication and pathogenicity. However, within APMV serotypes, significant genetic diversity exists, and the infectivity of variant strains in mammals has not been studied. We utilized a mouse model to evaluate the pathogenicity of a variant strain of APMV-6 (APMV-6/red-necked stint/Japan/8KS0813/2008) in comparison with the prototype APMV-6 strain (APMV-6/duck/Hong Kong/18/199/1977)...
July 6, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28674528/host-differences-affecting-resistance-and-susceptibility-of-the-second-generation-of-a-pekin-duck-flock-to-duck-hepatitis-a-virus-genotype-3
#16
Xiaoyan Wang, Jiaojiao Zhang, Runze Meng, Yong Jiang, Suyun Liang, Yunsheng Zhang, Ming Xie, Zhengkui Zhou, Shuisheng Hou
Earlier work suggested the possibility to anti duck hepatitis A virus genotype 3 (DHAV-3) using the resistance breeding strategy. Here, we report the creation of the second generations of a resistant Pekin duck flock (designated Z8R2) and a highly susceptible Pekin duck flock (designated Z8S2) and the investigation of their responses to DHAV-3. Experimental infection with DHAV-3 at 7 days of age resulted in a high mortality (66.3%) in 11 susceptible Z8S2 families and an extremely low mortality rate (2.67%) in 32 Z8R2 families, indicating that Z8R2 exhibits strong resistance to DHAV-3, while Z8S2 is highly susceptible to the virus...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28674238/characterization-of-duck-anas-platyrhynchos-mhc-class-i-gene-in-two-duck-lines
#17
Lin Zhang, Wei-Jie Liu, Jia-Qiang Wu, Min-Li Xu, Zheng-Jie Kong, Yan-Yan Huang, Shao-Hua Yang
To enrich gene polymorphism ofDuMHCI and provide data for further studies on disease resistance, 14DuMHCI genes from Weishan Ma duck and Cherry Valley duck were cloned, and their characterization were investigated. The overall conservation of the 14 alleles could be observed within the sequences, and relative conservation were also displayed in the peptide-binding domain and CD8 interaction sites. Based on full-length amino acid homology, MHC class I from different duck lines could be divided into 13 gene groups and three novel gene groups existed...
June 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28666650/functional-characterization-of-duck-lsm14a-in-ifn-%C3%AE-induction
#18
Kexin Hua, Huilin Li, Huanchun Chen, Mohamed Frahat Foda, Rui Luo, Hui Jin
Human LSm14A is a key component of processing body (P-body) assembly that mediates interferon-β (IFN-β) production by sensing viral RNA or DNA. To the best of our knowledge, we are the first to report duck LSm14A (duLSm14A) cloning from duck embryo fibroblasts (DEFs). Full-length duLSm14A encoded 461 amino acids and was highly homologous with chicken and swan goose sequences. More interestingly, the duLSm14A mRNA was extensively expressed in all the studied tissues. In DEFs, duLSm14A was localized in the cytoplasm as P-body-like dots...
June 27, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28640898/competition-between-influenza-a-virus-subtypes-through-heterosubtypic-immunity-modulates-re-infection-and-antibody-dynamics-in-the-mallard-duck
#19
Neus Latorre-Margalef, Justin D Brown, Alinde Fojtik, Rebecca L Poulson, Deborah Carter, Monique Franca, David E Stallknecht
Our overall hypothesis is that host population immunity directed at multiple antigens will influence the prevalence, diversity and evolution of influenza A virus (IAV) in avian populations where the vast subtype diversity is maintained. To investigate how initial infection influences the outcome of later infections with homologous or heterologous IAV subtypes and how viruses interact through host immune responses, we carried out experimental infections in mallard ducks (Anas platyrhynchos). Mallards were pre-challenged with an H3N8 low-pathogenic IAV and were divided into six groups...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28636622/inhibition-of-hepatitis-b-viral-entry-by-nucleic-acid-polymers-in-heparg-cells-and-primary-human-hepatocytes
#20
Clément Guillot, Nora Martel, Françoise Berby, Isabelle Bordes, Olivier Hantz, Matthieu Blanchet, Camille Sureau, Andrew Vaillant, Isabelle Chemin
Hepatitis B virus (HBV) infection remains a major public health concern worldwide with 240 million individuals chronically infected and at risk of developing cirrhosis and hepatocellular carcinoma. Current treatments rarely cure chronic hepatitis B infection, highlighting the need for new anti-HBV drugs. Nucleic acid polymers (NAPs) are phosphorothioated oligonucleotides that have demonstrated a great potential to inhibit infection with several viruses. In chronically infected human patients, NAPs administration lead to a decline of blood HBsAg and HBV DNA and to HBsAg seroconversion, the expected signs of functional cure...
2017: PloS One
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