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https://www.readbyqxmd.com/read/29469449/-investigation-on-sanitation-of-freshwater-aquaculture-environments-and-clonorchis-sinensis-intermediate-host-infection-in-a-city-of-pearl-river-delta-region-china
#1
Wang Man, Luo Le, Chen Xue-Qin, Li Lei, Fang Yue-Yi
OBJECTIVE: To understand the current status of the sanitation of freshwater aquaculture environments, and Clonorchis sinensis infection of freshwater fish in the aquaculture and market in a city of Pearl River Delta region, so as to provide the evidence for formulating the prevention and control strategy of clonorchiasis sinensis. METHODS: In 2016, based on the distribution of freshwater aquaculture, 36 freshwater fish ponds among 14 towns were selected for sampling and investigation, and 10-20 pieces were collected from each pond...
October 19, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29458524/ifn-and-cytokine-responses-in-ducks-to-genetically-similar-h5n1-influenza-a-viruses-of-varying-pathogenicity
#2
Leina B Saito, Laura Diaz-Satizabal, Danyel Evseev, Ximena Fleming-Canepa, Sai Mao, Robert G Webster, Katharine E Magor
Ducks, the reservoir host, are generally permissive to influenza A virus infection without disease symptoms. This natural ecology was upset by the emergence of H5N1 strains, which can kill ducks. To better understand host-virus interactions in the reservoir host, and influenza strain-specific molecular contributions to virulence, we infected White Pekin ducks with three similar H5N1 viruses, with known differences in pathogenicity and replication rate. We quantified viral replication and innate immune gene activation by qPCR, in lung and spleen tissues, isolated on each of the first 3 days of infection...
February 12, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29457857/towards-structural-studies-of-self-assembled-subviral-particles-combining-cell-free-expression-with-100-khz-mas-nmr
#3
Guillaume David, Marie-Laure Fogeron, Maarten Schledorn, Roland Montserret, Uta Haselmann, Susanne Penzel, Aurelie Badillo, Lauriane Lecoq, Patrice André, Michael Nassal, Ralf Bartenschlager, Beat H Meier, Anja Böckmann
Viral membrane proteins are prime targets in the combat against infection. Still, their structure determination remains a challenge, both with respect to sample preparation, and the need for structural methods allowing analysis in a native-like lipid environment. Cell-free protein synthesis and solid-state NMR are promising approaches in this context, one with respect to its high potential of native expression of complex proteins, and the other for its ability to analyze membrane proteins in lipids. We here show that milligram amounts of the small envelope protein of the duck hepatitis B virus (DHBV) can be produced using cell-free expression, and that the protein self-assembles into subviral particles...
February 19, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29422897/fowl-adenovirus-serotype-4-sd0828-infections-causes-high-mortality-rate-and-cytokine-levels-in-specific-pathogen-free-chickens-compared-to-ducks
#4
Rong Li, Gen Li, Jing Lin, Shaojie Han, Xiaolan Hou, Hongyu Weng, Mengjiao Guo, Zhong Lu, Ning Li, Yingli Shang, Tongjie Chai, Liangmeng Wei
Hydropericardium syndrome and inclusion body hepatitis, together called hydropericardium-hepatitis syndrome, are acute infectious diseases found in chickens. These diseases are caused primarily by fowl adenovirus serotype 4 (FAdV-4) strains. In this study, we isolated a FAdV-4 strain (SD0828) from clinically diseased chickens and phylogenetically analyzed the L1 loops of the hexon protein sequences in 3-week-old specific pathogen-free chickens and ducks infected intramuscularly and orally, determining differences in the pathogenicity by observing clinical signs and gross and histological lesions...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29408021/protective-efficacy-of-a-bivalent-live-attenuated-vaccine-against-duck-hepatitis-a-virus-types-1-and-3-in-ducklings
#5
Min Kang, Jae-Hee Roh, Hyung-Kwan Jang
Duck hepatitis A virus (DHAV) infection is characterized by an acute, rapidly spreading that affects young ducklings. DHAV-1 or DHAV-3 infection is prevalent, and simultaneous co-infection with both viruses has recently become increasingly frequent in the domestic duck farms. In this study, we developed a bivalent live attenuated vaccine (DHV-HSBP100 and AP-04203P100) for DHAV-1 and DHAV-3 and reported the protective efficacy and safety of the vaccine. At 1-day-old, the ducklings received a bivalent vaccine via intramuscular injection...
February 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29407574/pathogenicity-of-duck-hepatitis-a-virus-type-3-and-innate-immune-responses-of-the-ducklings-to-virulent-dhav-3
#6
Xuelian Zhang, Chong Cao, Zhehui Qu, Wenjing Zhang, Yue Liu, Haihui Qi, Chunxue Hao, Wenlong Zhang, Mingchun Gao, Junwei Wang, Bo Ma
Duck virus hepatitis caused by duck hepatitis A virus (DHAV) is an acute and contagious disease. To better understand the pathogenic mechanism of DHAV-3 in ducklings, an infection experiment was performed. Our results showed that typical symptoms were observed in the infected ducklings. DHAV-3 could infect many tissues, leading to pathological lesions, especially on the livers and spleen, and the host immune responses are activated in infection. Real-time quantitative PCR demonstrated that expression of many innate immune-related genes was mostly up-regulated in the livers and spleen, and antiviral innate immune response was established, but not sufficient to restrict the virus replication of lethal dose...
January 26, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29407573/identification-of-duck-il-4-and-its-inhibitory-effect-on-il-17a-expression-in-r-anatipestifer-stimulated-splenic-lymphocytes
#7
Cherry P Fernandez, Fahmida Afrin, Rochelle A Flores, Woo H Kim, Jipseol Jeong, Suk Kim, Hyun S Lillehoj, Wongi Min
As the dysregulation of IL-17 is implicated in the pathogenesis of various autoimmune and inflammatory diseases, the suppression of IL-17 production by Th2 cytokines could alleviate the development of these diseases. Previously, we confirmed that inflammatory cytokines including IL-17A are strongly associated with R. anatipestifer infection, which is one of the most important bacterial pathogens in the duck industry. Here, we found that IL-4 treatment downregulated the expression of IL-17A and IL-17F transcripts in splenic lymphocytes stimulated with R...
February 2, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29406083/a-targeted-investigation-to-demonstrate-the-freedom-of-west-timor-from-hpai-h5n1
#8
Petrus Malo Bulu, Ian D Robertson, Maria Geong
In early 2004 highly pathogenic avian influenza (HPAI) H5N1 virus caused major outbreaks of disease in poultry in Indonesia. The disease was first reported in West Timor in eastern Indonesia in the same year, resulting in the death of approximately one hundred chickens from both commercial and backyard farms; however no evidence of disease has subsequently been reported in West Timor since 2007. A targeted survey was undertaken in 2013 in 2 districts of West Timor. Three hundred village and commercial poultry (292 chickens and 8 Muscovy ducks) from 10 villages and 5 live bird markets (LBMs) were sampled between August and October 2013...
February 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29403654/seroprevalence-of-chlamydophila-psittaci-among-employees-of-two-german-duck-farms
#9
Raimond Lugert, Uwe Groß, Wycliffe O Masanta, Gunter Linsel, Astrid Heutelbeck, Andreas E Zautner
Psittacosis is a zoonotic infectious disease that is caused by Chlamydophila psittaci. To determine the occupational risk of getting the infection, we investigated the seroprevalence of C. psittaci among employees of two German duck farms and two slaughterhouses according to their level of exposure to the pathogen during the years 2010, 2007, and 2004. In summary, we found low seroprevalence (≈ 8%) throughout the study population almost irrespective of the duty of a given worker. Surprisingly, in 2010, the anti-C...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29402276/establishment-of-a-yeast-based-vlp-platform-for-antigen-presentation
#10
David Wetzel, Theresa Rolf, Manfred Suckow, Andreas Kranz, Andreas Barbian, Jo-Anne Chan, Joachim Leitsch, Michael Weniger, Volker Jenzelewski, Betty Kouskousis, Catherine Palmer, James G Beeson, Gerhard Schembecker, Juliane Merz, Michael Piontek
BACKGROUND: Chimeric virus-like particles (VLP) allow the display of foreign antigens on their surface and have proved valuable in the development of safe subunit vaccines or drug delivery. However, finding an inexpensive production system and a VLP scaffold that allows stable incorporation of diverse, large foreign antigens are major challenges in this field. RESULTS: In this study, a versatile and cost-effective platform for chimeric VLP development was established...
February 5, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29399081/evaluation-of-the-inactivation-effect-of-riboflavin-photochemical-method-on-duck-hepatitis-b-virus
#11
Zi-Yu Zhou, Xing-Xiu Bi
Hepatitis B virus (HBV) is a common and widespread infection that poses a serious threat among carriers for the development of life-threatening liver diseases. The aim of the present study was to evaluate the effectiveness of the riboflavin photochemical method in inactivating duck hepatitis B virus (DHBV) in plasma via an animal model. Forty ducks were selected and randomly divided into the experimental (n=10), the virus control (n=10), the visible light control (n=10) and the plasma control group (n=10). Ducks in the experimental group were injected with plasma inactivated by the riboflavin photochemical method; in the virus control group were injected with plasma without inactivation treatment; in the visible light control group were injected with plasma irradiated by visible light; and in the plasma control group were injected with normal plasma...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29397437/microphallus-ochotensis-sp-nov-digenea-microphallidae-and-relative-merits-of-two-host-microphallid-life-cycles
#12
Kirill V Galaktionov, Isabel Blasco-Costa
A new digenean species, Microphallus ochotensis sp. nov., was described from the intestine of Pacific eiders (Somateria mollissima v-nigrum) from the north of the Sea of Okhotsk. It differs from other microphallids in the structure of the metraterm, which consists of two distinct parts: a sac with spicule-like structures and a short muscular duct opening into the genital atrium. Mi. ochotensis forms a monophyletic clade together with other congeneric species in phylograms derived from the 28S and ITS2 rRNA gene...
February 3, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29391035/comparative-genetic-analysis-and-pathological-characteristics-of-goose-parvovirus-isolated-in-heilongjiang-china
#13
Yinjie Niu, Lili Zhao, Baihan Liu, Jingli Liu, Fan Yang, Haichang Yin, Hong Huo, Hongyan Chen
BACKGROUND: Goose parvovirus (GPV) causes acute enteritis, hepatitis, myocarditis and high morbidity and mortality in geese and ducks. GPV H strain was isolated from a Heilongjiang goose farm where the geese were showing signs of hemorrhage in the brain, liver, and intestinal tract. In this study, we explored the genetic diversity among waterfowl parvovirus isolates and the pathological characteristics of GPV H in Shaoxing ducklings. METHODS: The complete capsid protein (VP) and non-structural (NS) sequences of the isolated H strain were sequenced, and phylogenetic trees of VP and NS were constructed in MEGA version 5...
February 1, 2018: Virology Journal
https://www.readbyqxmd.com/read/29370857/environmental-resistance-development-to-influenza-antivirals-a-case-exemplifying-the-need-for-a-multidisciplinary-one-health-approach-including-physicians
#14
REVIEW
Josef D Järhult
A multidisciplinary approach is a prerequisite for One Health. Physicians are important players in the One Health team, yet they are often hard to convince of the benefits of the One Health approach. Here, the case for multidisciplinarity including physicians is made using the example of environmental resistance development to influenza antivirals. Neuraminidase inhibitors are the major class of anti-influenza pharmaceuticals, and extensively stockpiled globally as a cornerstone of pandemic preparedness, especially important in the first phase before vaccines can be mass-produced...
January 25, 2018: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29366299/identification-sequence-analysis-and-infectivity-of-h9n2-avian-influenza-viruses-isolated-from-geese
#15
Rui Zhu, Xueqin Yang, Jianjun Zhang, Danwen Xu, Jiawen Fan, Huoying Shi, Shifeng Wang, Xiufan Liu
H9N2 subtype avian influenza virus is a great threat to the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three strains of the H9N2 avian influenza virus, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the Gs/JS/YZ527/11 virus is a double-reassortant virus, combining the genes of A/Quail/Hong Kong/G1/97 (H9N2, G1/97) - like and A/Chicken/Shanghai/F/98 (H9N2, F/98) - like; the Gs/JS/SQ119/12 virus is a triple-reassortant virus derived from G1/97 - like, F/98 - like, and A/Duck/Shantou/163/2004 (H9N2, ST/163/04) - like...
January 25, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29363279/an-egyptian-hpai-h5n1-isolate-from-clade-2-2-1-2-is-highly-pathogenic-in-an-experimentally-infected-domestic-duck-breed-sudani-duck
#16
M Samir, M Hamed, F Abdallah, V Kinh Nguyen, E A Hernandez-Vargas, F Seehusen, W Baumgärtner, A Hussein, A A H Ali, F Pessler
The highly pathogenic avian influenza (HPAI) H5N1 viruses continue to cause major problems in poultry and can, although rarely, cause human infection. Being enzootic in domestic poultry, Egyptian isolates are continuously evolving, and novel clades vary in their pathogenicity in avian hosts. Considering the importance of domestic ducks as natural hosts of HPAI H5N1 viruses and their likelihood of physical contact with other avian hosts and humans, it is of utmost importance to characterize the pathogenicity of newly emerged HPAI strains in the domestic duck...
January 24, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29350140/co-circulation-of-influenza-a-h5-h7-and-h9-viruses-and-co-infected-poultry-in-live-bird-markets-cambodia
#17
Paul F Horwood, Srey Viseth Horm, Annika Suttie, Sopheak Thet, Phalla Y, Sareth Rith, San Sorn, Davun Holl, Sothyra Tum, Sowath Ly, Erik A Karlsson, Arnaud Tarantola, Philippe Dussart
Longitudinal surveillance of 2 live bird markets in Cambodia revealed year-round, high co-circulation of H5, H7, and H9 influenza viruses. We detected influenza A viruses in 51.3% of ducks and 39.6% of chickens, and co-infections, mainly by H5 and H9 viruses, in 0.8% of ducks and 4.5% of chickens.
February 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29349092/cathepsin-b-plays-a-key-role-in-optimal-production-of-the-influenza-a-virus
#18
Macon D Coleman, Soon-Duck Ha, S M Mansour Haeryfar, Stephen Dominic Barr, Sung Ouk Kim
Background: Influenza A virus (IAV) is the etiologic agent of the febrile respiratory illness, commonly referred to as 'flu'. The lysosomal protease cathepsin B (CTSB) has shown to be involved in the lifecycle of various viruses. Here, we examined the role of CTSB in the IAV lifecycle. Methods: CTSB-deficient (CTSB-/-) macrophages and the human lung epithelial cell line A549 cells treated with CA-074Me were infected with the A/Puerto Rico/8/34 strain of IAV (IAV-PR8)...
2018: Journal of Virology & Antiviral Research
https://www.readbyqxmd.com/read/29341432/duck-beak-atrophy-and-dwarfism-syndrome-disease-complex-interplay-of-novel-goose-parvovirus-related-virus-and-duck-circovirus
#19
P Li, J Li, R Zhang, J Chen, W Wang, J Lan, Z Xie, S Jiang
As a newly emerged infectious disease, duck "beak atrophy and dwarfism syndrome (BADS)" disease has caused huge economic losses to waterfowl industry in China since 2015. Novel goose parvovirus-related virus (NGPV) is believed the main pathogen of BADS disease; however, BADS is rarely reproduced by infecting ducks with NGPV alone. As avian circovirus infection causes clinical symptoms similar to BADS, duck circovirus (DuCV) is suspected the minor pathogen of BADS disease. In this study, an investigation was carried out to determine the coinfection of NGPV and DuCV in duck embryos and in ducks with BADS disease...
January 16, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29334975/molecular-characterization-of-duck-enteritis-virus-ul41-protein
#20
Tianqiong He, Mingshu Wang, Xuelian Cao, Anchun Cheng, Ying Wu, Qiao Yang, Mafeng Liu, Dekang Zhu, Renyong Jia, Shun Chen, Kunfeng Sun, Xinxin Zhao, Xiaoyue Chen
BACKGROUND: Duck enteritis virus (DEV) belongs to the subfamily Alphaherpesvirinae, and information on the DEV UL41 gene is limited. METHODS: The DEV UL41 gene was cloned into the pET32a(+) vector and expressed in a prokaryotic expression system. Antiserum was raised against a bacterially expressed UL41-His fusion protein for further experiments. Transcription was quantified and UL41 protein expression levels were determined in DEV-infected cells at different time points by RT-qPCR and western blotting, respectively...
January 15, 2018: Virology Journal
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